{
  "@context": {
    "@vocab": "https://schema.org/"
  },
  "@type": "DigitalDocument",
  "@id": "https://raw.githubusercontent.com/BeBOP-OBON/TechOceanS_protocol_collection/main/odis_metadata/Colorimetric_LAMP.json",
  "hasDigitalDocumentPermission": "embargoed",
  "name": "Real-time colorimetric loop-mediated isothermal amplification (LAMP): Standard Operating Procedure",
  "description": "A standard operating procedure to carry out real-time LAMP",
  "url": "https://oceandecade.org/actions/better-biomolecular-ocean-practices/",
  "author": {
    "@id": " https://orcid.org/0000-0002-4099-5394",
    "@type": "Person",
    "name": "Katherine Hartle-Mougiou",
    "givenName": "Katherine",
    "familyName": "Hartle-Mougiou"
  },
  "contributor": {
    "@type": "Person",
    "@id": "https://orcid.org/0000-0003-0074-554X",
    "name": "Martha Valiadi",
    "givenName": " Martha",
    "familyName": "Valiadi"
  },
  "identifier": "",
  "keywords": [
    "Colorimetric loop-mediated isothermal amplification",
    "Real-time",
    "DNA amplification",
    "Protocol",
    "LAMP",
    "SOP"
  ],
  "provider": {
    "@type": "Organization",
    "legalName": "FOUNDATION FOR RESEARCH AND TECHNOLOGY - HELLAS (FO.R.T.H.)",
    "name": "Institute of Molecular Biology and Biotechnology - IMBB",
    "url": " https://www.forth.gr/en/home/"
  },
  "license": "CC-BY",
  "publisher": {
    "@type": "Organization",
    "legalName": "Better Biomolecular Ocean Protocols",
    "url": " https://github.com/BeBOP-OBON"
  },
  "about": {
    "@type": "Action",
    "actionStatus": "PotentialActionStatus",
    "name": "Colorimetric loop-mediated isothermal amplification (LAMP)",
    "instrument": [
      "IRIS prototype or Pebble device (BIOPIX-T)",
      "Vortex",
      "centrifuge"
    ],
    "location": "This protocol can be applied in a laboratory equipped for molecular biology testing",
    "object": [
      "PCR tubes",
      "WarmStart Colorimetric LAMP 2X Master Mix",
      "mineral oil",
      "DNA",
      "molecular grade water",
      "oligonucleotide primers",
      "Pterois miles (lionfish) COI primers",
      "Pseudo-nitzchia multistriata DabA primers"
    ],
    "result": [
      "Change in colour index, indicating DNA amplification",
      "Colorimetric signal",
      "Light signal"
    ]
  }
}