{
  "@context": {
    "@vocab": "https://schema.org/"
  },
  "@type": "DigitalDocument",
  "@id": "https://raw.githubusercontent.com/BeBOP-OBON/TechOceanS_protocol_collection/main/odis_metadata/crude_DNA_isolation.json",
  "hasDigitalDocumentPermission": "embargoed",
  "name": "Protocol for crude DNA isolation from phytoplankton: Standard Operating Procedure",
  "description": "A standard operating procedure for the crude DNA isolation from phytoplankton cells",
  "url": "https://oceandecade.org/actions/better-biomolecular-ocean-practices/",
  "author": {
    "@id": " https://orcid.org/0000-0002-4099-5394",
    "@type": "Person",
    "name": "Katherine Hartle-Mougiou",
    "givenName": "Katherine",
    "familyName": "Hartle-Mougiou"
  },
  "contributor": {
    "@type": "Person",
    "@id": "https://orcid.org/0000-0003-0074-554X",
    "name": "Martha Valiadi",
    "givenName": " Martha",
    "familyName": "Valiadi"
  },
  "identifier": "",
  "keywords": [
    "crude DNA isolation",
    "phytoplankton",
    "GuHCl",
    "Protocol",
    "cell culture",
    "magnetic beads",
    "SOP"
  ],
  "provider": {
    "@type": "Organization",
    "legalName": "FOUNDATION FOR RESEARCH AND TECHNOLOGY - HELLAS (FO.R.T.H.)",
    "name": "Institute of Molecular Biology and Biotechnology - IMBB",
    "url": " https://www.forth.gr/en/home/"
  },
  "license": "CC-BY",
  "publisher": {
    "@type": "Organization",
    "legalName": "Better Biomolecular Ocean Protocols",
    "url": " https://github.com/BeBOP-OBON"
  },
  "about": {
    "@type": "Action",
    "actionStatus": "PotentialActionStatus",
    "name": "Protocol for crude DNA isolation from phytoplankton",
    "instrument": [
      "pipette",
      "magnetic rack",
      "centrifuge"
    ],
    "location": "This protocol can be applied in a laboratory equipped for molecular biology testing",
    "object": [
      "GuHCl",
      "Seawater",
      "cell culture",
      "DNA",
      "molecular grade water",
      "magnetic beads",
      "1.5ml tubes",
      "isopropanol"
    ],
    "result": "Crude isolation of phytoplankton DNA"
  }
}