Draft version CLO-NICR: Cell Line Ontology Subset for Chinese National Infrastructure of Cell Line Resource (NICR) Sheng Yang Xiaocui Bian Yuqin Liu Hongjie Pan Xiaolin Yang Yongqun He 2018-06-17 A Chinese-English bilinggual subset for CLO, designed for cells in Chinese National Infrastructure of Cell Line Resource (NICR). OWL-DL An ontology for the domain of cell lines and cell line cells 2.1.135 BFO OWL specification label BFO OWL规范标签 Relates an entity in the ontology to the name of the variable that is used to represent it in the code that generates the BFO OWL file from the lispy specification. 将本体中的实体关联到用于在代码中表示它的变量的名称，这些代码从lispy规范生成BFO OWL 文件。 Really of interest to developers only 只对开发人员感兴趣 BFO OWL specification label BFO OWL规范标签 BFO OWL规范标签 BFO CLIF specification label BFO CLIF规范标签 Relates an entity in the ontology to the term that is used to represent it in the the CLIF specification of BFO2 将本体中的实体关联到用于在BFO2 CLIF规范中表示它的术语上。 Person:Alan Ruttenberg Person:Alan Ruttenberg Really of interest to developers only 只对开发人员感兴趣 BFO CLIF specification label BFO CLIF规范标签 BFO CLIF规范标签 An annotation property that represents an LINCS ID of a cell line. 表示细胞系的LINCS ID的注释属性。 Oliver He, Jiangan Xie Oliver He, Jiangan Xie Cell line LINCS ID 细胞系LINCS ID 细胞系LINCS ID Yongqun He Yongqun He An annotation property that specifies an STR profile of a cell line cell. 用于说明细胞系的STR图谱的注释属性。 STR profile STR图谱 An annotation property that describes the information of a problematic cell line. 描述有问题的细胞系信息的注释属性。 Yongqun He Yongqun He problematic cell line annotation 问题细胞注释 An annotation property that describes the Resource ID of the cells in National Infrastructure of Cell Line Resource. 描述“国家实验细胞资源共享平台”中细胞的资源ID的注释属性。 Hongjie Pan Hongjie Pan Resource ID 资源ID An annotation property that describes the English full name of a cell line. 描述细胞系中文名称的注释属性。 Hongjie Pan Hongjie Pan Chinese standardized name 中文名称 An annotation property that describes the morphological characteristics of a cell line. 描述细胞系形态特征的注释属性。 Hongjie Pan Hongjie Pan Morphological Characteristics 形态特征 An annotation property that describes the growth characteristics of a cell line. 描述细胞系生长特性的注释属性。 Hongjie Pan Hongjie Pan Growth Characteristics 生长特性 An annotation property that describes the general characteristics of a cell line. 描述细胞系基本特性的注释属性。 Hongjie Pan Hongjie Pan specific characteristics 特征特性 An annotation property that describes the culture conditions of a cell line. 描述细胞系培养条件的注释属性。 Hongjie Pan Hongjie Pan culture conditions 培养条件 An annotation property that describes the passage situation of a cell line. 描述细胞系传代情况的注释属性。 Hongjie Pan Hongjie Pan passage situation 传代情况 An annotation property that describes the frozen storage conditions of a cell line. 描述细胞系冷冻储存条件的注释属性。 Hongjie Pan Hongjie Pan Frozen Storage Conditions 冻存条件 An annotation property that describes the mycoplasma detection of a cell line. 描述支原体检测细胞系的注释属性。 Hongjie Pan Hongjie Pan mycoplasma detection 支原体检测 An annotation property that describes the isozyme analysis of a cell line. 描述细胞系同工酶分析的注释属性。 Hongjie Pan Hongjie Pan Isozyme Analysis 同工酶分析 An annotation property that describes the chromosome style of a cell line. 描述细胞系染色体类型的注释属性。 Hongjie Pan Hongjie Pan chromosome style 染色体类型 An annotation property that describes the application permission of a cell line. Cells in class A can be free distributed; cells in class B can be distributed with the permission of the donar; cells in Class C should be safety deposited 描述细胞系使用权限的注释属性。 Hongjie Pan Hongjie Pan application permission 使用权限 An annotation property that describes the picture of a cell line. 描述细胞系图片的注释属性。 Hongjie Pan Hongjie Pan picture 图片 An annotation property that describes the depository of a cell line. 描述细胞系保藏的注释属性。 Hongjie Pan Hongjie Pan depository 保藏单位 An annotation property that describes the contact information of a cell line. 描述细胞系联系人信息的注释属性。 Hongjie Pan Hongjie Pan contacts 联系方式 An annotation property that describes the passage method of a cell line. 描述细胞系传代方法的注释属性。 Hongjie Pan Hongjie Pan passage method 传代方法 editor preferred label editor preferred label editor preferred term editor preferred term editor preferred term~editor preferred label 编辑首选术语 编辑首选标签 The concise, meaningful, and human-friendly name for a class or property preferred by the ontology developers. (US-English) 对于一类或属性的简洁的、有意义的、与人类友好的名称由本体开发商首选。 （美国英语） PERSON:Daniel Schober GROUP:OBI:<http://purl.obolibrary.org/obo/obi> editor preferred label editor preferred label editor preferred term editor preferred term editor preferred term~editor preferred label editor preferred term~editor preferred label 编辑首选术语 编辑首选术语 编辑首选术语~编辑首选标签 编辑首选标签 编辑首选标签 example example of usage 例子 用法示例 A phrase describing how a class name should be used. May also include other kinds of examples that facilitate immediate understanding of a class semantics, such as widely known prototypical subclasses or instances of the class. Although essential for high level terms, examples for low level terms (e.g., Affymetrix HU133 array) are not A phrase describing how a term should be used and/or a citation to a work which uses it. May also include other kinds of examples that facilitate immediate understanding, such as widely know prototypes or instances of a class, or cases where a relation is said to hold. 短语描述一个类的名字应该如何使用。也可包括其他类型有助于直接理解类的语义的例子，如广为人知的类的原型子类或实例。虽然高水平的术语必不可少，对于低级别术语的示例（例如，Affymetrix HU133阵列）不 PERSON:Daniel Schober GROUP:OBI:<http://purl.obolibrary.org/obo/obi> example of usage example of usage 用法示例 用法示例 has curation status 有管理状态 PERSON:Alan Ruttenberg PERSON:Bill Bug PERSON:Melanie Courtot OBI_0000281 has curation status has curation status 有管理状态 有管理状态 definition definition textual definition textual definition 定义 OBI的官方定义，解释类或属性的含义。应该是亚里士多德式的，形式化和规范化的。 可以通过口语定义进行扩充。 The official OBI definition, explaining the meaning of a class or property. Shall be Aristotelian, formalized and normalized. Can be augmented with colloquial definitions. The official definition, explaining the meaning of a class or property. Shall be Aristotelian, formalized and normalized. Can be augmented with colloquial definitions. 官方的定义，解释类或属性的含义。应该是亚里士多德式的，形式化和规范化的。 可以通过口语定义进行扩充。 2012-04-05: Barry Smith OBI的官方定义解释了一个类或属性的含义：'应该是亚里士多德式的，形式化和规范化的。可以用口语定义'是糟糕的。 您能解决这样的问题吗？ 解释关于类或属性的表达含义的必要和充分条件的陈述。 Alan Ruttenberg 您提出的定义是一个合理的备选，除非它很常见，没有给出必要和充分的条件。大多数情况下它们是必要的，偶尔是必要的，充分的或者仅仅仅充分的。它们通常使用不是自己定义的术语，因此它们实际上不能通过这些标准进行评估。 关于拟议定义的具体内容： 我们没有“含义”，或“表达”或“属性”的定义。对于在预期意义上的“参考”，我认为我们使用术语“指示”。对于'表达'，我认为我们和你的意思是符号，或标识符。对于“含义”，它不同于类和属性。对于类，我们希望文档能够让读者确定一个实体是否是该类的实例。对于属性，让我们的目标读者决定，给定一对潜在的关系，判断关系成立的断言正确与否。 “目标读者”部分表明我们也指定了我们期望的能够理解定义的人，并且概括了人类和计算机读者以包含文本和逻辑定义。 就我个人而言，我更愿意削弱对文档的定义，并指出什么是可取的。 我们还有一个悬而未决的问题，就是如何针对不同的受众定位不同的定义。临床读者阅读chebi需要来自受过化学训练的受众的不同类型的定义文档/定义，同样需要一个适合本体工作者的定义。 2012-04-05: Barry Smith The official OBI definition, explaining the meaning of a class or property: 'Shall be Aristotelian, formalized and normalized. Can be augmented with colloquial definitions' is terrible. Can you fix to something like: A statement of necessary and sufficient conditions explaining the meaning of an expression referring to a class or property. Alan Ruttenberg Your proposed definition is a reasonable candidate, except that it is very common that necessary and sufficient conditions are not given. Mostly they are necessary, occasionally they are necessary and sufficient or just sufficient. Often they use terms that are not themselves defined and so they effectively can't be evaluated by those criteria. On the specifics of the proposed definition: We don't have definitions of 'meaning' or 'expression' or 'property'. For 'reference' in the intended sense I think we use the term 'denotation'. For 'expression', I think we you mean symbol, or identifier. For 'meaning' it differs for class and property. For class we want documentation that let's the intended reader determine whether an entity is instance of the class, or not. For property we want documentation that let's the intended reader determine, given a pair of potential relata, whether the assertion that the relation holds is true. The 'intended reader' part suggests that we also specify who, we expect, would be able to understand the definition, and also generalizes over human and computer reader to include textual and logical definition. Personally, I am more comfortable weakening definition to documentation, with instructions as to what is desirable. We also have the outstanding issue of how to aim different definitions to different audiences. A clinical audience reading chebi wants a different sort of definition documentation/definition from a chemistry trained audience, and similarly there is a need for a definition that is adequate for an ontologist to work with. PERSON:Daniel Schober GROUP:OBI:<http://purl.obolibrary.org/obo/obi> definition definition textual definition textual definition 定义 定义 文本定义 文本定义 editor note 编者注 An administrative note intended for its editor. It may not be included in the publication version of the ontology, so it should contain nothing necessary for end users to understand the ontology. 管理者注释用于其编辑器。它可能不包含在本体的出版版本中，所以它应该不包含最终用户了解本体所需的信息。 PERSON:Daniel Schober GROUP:OBI:<http://purl.obfoundry.org/obo/obi> IAO:0000116 uberon editor_note 编辑_注释 true editor_note 编辑_注释 IAO:0000116 uberon editor_note 1 editor_note editor note editor note 编者注 编者注 definition editor term editor 术语编辑者 Name of editor entering the definition in the file. The definition editor is a point of contact for information regarding the term. The definition editor may be, but is not always, the author of the definition, which may have been worked upon by several people Name of editor entering the term in the file. The term editor is a point of contact for information regarding the term. The term editor may be, but is not always, the author of the definition, which may have been worked upon by several people 输入文件中术语编辑者的名称。术语编辑者是有关术语的信息交汇点。术语编辑者可以是，但并不总是，定义的作者，这可能是由几个人完成 20110707, MC: label update to term editor and definition modified accordingly. See http://code.google.com/p/information-artifact-ontology/issues/detail?id=115. 20110707, MC: label update to term editor and definition modified accordingly. See https://github.com/information-artifact-ontology/IAO/issues/115. 20110707，MC：术语编辑和定义进行相应的修改。见http://code.google.com/p/information-artifact-ontology/issues/detail?id=115。 PERSON:Daniel Schober GROUP:OBI:<http://purl.obolibrary.org/obo/obi> definition editor definition editor term editor term editor 术语编辑者 术语编辑者 alternative term 替代术语 An alternative name for a class or property which means the same thing as the preferred name (semantically equivalent) 一类或属性的替代名称，这意味着与所述首选名称同样的事物（语义上等价） PERSON:Daniel Schober GROUP:OBI:<http://purl.obolibrary.org/obo/obi> alternative term 替代术语 替代术语 definition source 定义来源 formal citation, e.g. identifier in external database to indicate / attribute source(s) for the definition. Free text indicate / attribute source(s) for the definition. EXAMPLE: Author Name, URI, MeSH Term C04, PUBMED ID, Wiki uri on 31.01.2007 正式引用，例如在外部数据库中的标识符，用于表示/定义的属性源（S）。自由文本表示/为定义属性源（S）。实例：在2007年1月31日的作者姓名，URI，MeSH术语C04，PUBMEDID，维基uri PERSON:Daniel Schober Discussion on obo-discuss mailing-list, see http://bit.ly/hgm99w GROUP:OBI:<http://purl.obolibrary.org/obo/obi> 论OBO-讨论邮件-列表，请参阅http://bit.ly/hgm99w definition source definition source 定义来源 定义来源 curator note 管理者注释 An administrative note of use for a curator but of no use for a user 管理者注释对管理者有用对用户无用 PERSON:Alan Ruttenberg IAO:0000232 uberon curator_notes 管理者_注释 true curator_notes 管理者_注释 curator note curator notes 管理者注释 管理者注释 imported from 引自 For external terms/classes, the ontology from which the term was imported 外部术语/类，术语引入的本体 PERSON:Alan Ruttenberg PERSON:Melanie Courtot GROUP:OBI:<http://purl.obolibrary.org/obo/obi> imported from imported from 引自 引自 elucidation 说明 person:Alan Ruttenberg Person:Barry Smith Primitive terms in a highest-level ontology such as BFO are terms which are so basic to our understanding of reality that there is no way of defining them in a non-circular fashion. For these, therefore, we can provide only elucidations, supplemented by examples and by axioms 在最高级别的本体中的原始术语如BFO是对我们理解现实最基本，以至于无法以非循环的方式来定义它们的术语。对于这些，因此，我们只能提供说明，通过例子和公理进行补充 elucidation 说明 说明 has associated axiom(nl) 具有相关联的公理（nl） Person:Alan Ruttenberg Person:Alan Ruttenberg An axiom associated with a term expressed using natural language 与一个使用自然语言表达的术语相关的公理 has associated axiom(nl) 具有相关联的公理（nl） 具有相关联的公理（nl） has associated axiom(fol) 具有相关联的公理（fol） Person:Alan Ruttenberg Person:Alan Ruttenberg An axiom expressed in first order logic using CLIF syntax 使用CLIF语法在第一逻辑进行表达的公理 has associated axiom(fol) 具有相关联的公理（fol） 具有相关联的公理（fol） NHC0 code code code The semantic type describes the sort of thing or category to which a concept belongs in the context of the UMLS semantic network. P106 Conceptual Entity Semantic Type Semantic_Type In general, applying semantic types aids in allowing users (or computer programs) to draw conclusions about concepts by virtue of the categories to which they have been assigned. We use a set of semantic types developed for the UMLS Metathesaurus. There are currently 134 semantic types in the UMLS. Semantic_Type Semantic_Type Provides an alternative Preferred Name for use in some NCI systems. P107 Conceptual Entity Display Name Display_Name Display Name Display_Name Display_Name The word or phrase that NCI uses by preference to refer to the concept. P108 Conceptual Entity Preferred Name Preferred_Name Preferred Name Preferred Term Preferred_Name Preferred_Name Concept Unique Identifiers, or CUIs, are concept numbers assigned by the National Library of Medicine (NLM). If a concept in any NCI-maintained knowledgebase exists in the NLM Unified Medical Language System (UMLS), NCI includes the NLM CUI among the information we provide about the concept. P207 Conceptual Entity UMLS CUI UMLS_CUI UMLS_CUI UMLS_CUI This property is used to indicate when a non-EVS entity has contributed to, and has a stake in, a concept. This is used where such entities, within or outside NCI, have indicated the need to be able to track their own concepts. A single concept can have multiple instances of this property if multiple entities have such a defined stake. P322 Conceptual Entity Contributing Source Contributing_Source Contributing_Source Contributing_Source English language definitions of what a source other than NCI means by the concept. These are limited to 1024 characters. They include information about the definition's source in a form that can easily be interpreted by software. P325 Conceptual Entity [source] Definition ALT_DEFINITION ALT_DEFINITION ALT_DEFINITION A retired unique concept identifier created and stored as Concept Name by legacy EVS software. Use of these values was long discouraged, but continued as late as 2009 when creation of new values ceased and Concept Name was retired. Legacy values are intended solely to help resolve and update earlier coding. P366 Conceptual Entity Legacy Concept Name Legacy Concept Name Legacy_Concept_Name Currently used by MMHCC to identify the source of various mouse disease concepts. P92 Conceptual Entity Subsource Subsource Subsource Subsource the symbol assigned by the nomenclature authority Oliver He, Yue Liu symbol from nomenclature authority the full name assigned by the nomenclature authority Oliver He, Yue Liu full name from nomenclature authority A GeneID in the NCBI Gene database Oliver He, Yue Liu NCBI GeneID the NCBI LocusTag name of a gene Oliver He, Yue Liu NCBI LocusTag the map location of a gene Oliver He, Yue Liu gene map location a date of content modification Oliver He, Yue Liu modification date The NCBITaxon ontology ID of an organism. Oliver He, Yue Liu organism NCBITaxon ID A chromosome ID where a gene is located. Oliver He chromosome ID of gene an annotation property that specifies the type of a gene Oliver He type of gene an annotation property that specifies a nomenclature status Oliver He nomenclature status an annotation property that shows the GO information associated with a specific gene. Yongqun He YH: use the convention: GO_ID (EC: xx; Qualifier: xx; PMID: xxxxx;) where GO_ID is a GO ID, EC is the Evidence Code, Qualifier is a specific association type, and PMID is a PubMed ID of a paper that supports the gene-GO association. has GO association An annotation property that represents a gene's association with PubMed publication(s). Yongqun He YH: use the format: PMID: pmid1, pmid2, ... where pmid1 and pmid2 are specfic PubMed IDs (PMIDs). has PubMed association RO:0002174 uberon dubious_for_taxon 可疑_于_分类 true true dubious_for_taxon 可疑_于_分类 this relation lacks a strong logical interpretation, but can be used in place of never_in_taxon where it is desirable to state that the definition of the class is too strict for the taxon under consideration, but placing a never_in_taxon link would result in a chain of inconsistencies that will take time to resolve. Example: metencephalon in teleost 这种关系缺乏一个强有力的逻辑解释，但可以以理想状态用在不_在_分类中，其中有必要说明该类的定义对于所考虑的分类群来说过于严格，但放置一个不_在_分类链接会导致链的不一致，需要时间来解决。例如：后脑硬骨 dubious_for_taxon 可疑_于_分类 可疑_于_分类 S dubious_for_taxon T if it is probably the case that no instances of S can be found in any instance of T. S可疑_于_分类 T如果在T的任何实例中可能找不到S的实例， RO:0002175 applicable for taxon 适用于分类单元 uberon present_in_taxon 存在_于_分类 true true present_in_taxon 存在_于_分类 present_in_taxon 存在_于_分类 存在_于_分类 S present_in_taxon T if some instance of T has some S. This does not means that all instances of T have an S - it may only be certain life stages or sexes that have S S存在_于_分类 T如果T的某个实例有一些S，这并不意味着T的所有实例都有S - 它可能只是某些生命阶段或者性别具有S UBPROP:0000001 uberon external_definition 外部_定义 true external_definition 外部_定义 This annotation property may be replaced with an annotation property from an external ontology such as IAO 这个注释的属性可以用从外部本体如IAO注解属性替换 external_definition 外部_定义 外部_定义 An alternate textual definition for a class taken unmodified from an external source. This definition may have been used to derive a generalized definition for the new class. 对外部来源未修改的类的替代文本定义。这个定义可能被用来为新类推导出一个广义的定义。 UBPROP:0000002 uberon axiom_lost_from_external_ontology 公理_丢失_自_外部_本体 true axiom_lost_from_external_ontology 公理_丢失_自_外部_本体 This annotation property may be replaced with an annotation property from an external ontology such as IAO 这个注释的属性可以用从外部本体如IAO注解属性替换 axiom_lost_from_external_ontology 公理_丢失_自_外部_本体 公理_丢失_自_外部_本体 A textual description of an axiom loss in this ontology compared to an external ontology. 与外部本体相比，本本体中的公理缺失的文本描述。 UBPROP:0000003 uberon homology_notes 同源_注释 true homology_notes 同源_注释 This annotation property may be replaced with an annotation property from an external ontology such as IAO 这个注释的属性可以用从外部本体如IAO注解属性替换 homology_notes 同源_注释 同源_注释 Notes on the homology status of this class. 关于这个类的同源性状态的注释。 UBPROP:0000007 uberon has_relational_adjective 有_关系的_形容词 true has_relational_adjective 有_关系的_形容词 has_relational_adjective 有_关系_形容词 有_关系_形容词 Used to connect a class to an adjectival form of its label. For example, a class with label 'intestine' may have a relational adjective 'intestinal'. 用于将类连接到其标签的形容词形式。例如，具有标签“肠”的类可能具有关系形容词“肠的”。 UBPROP:0000008 uberon taxon_notes 分类_注释 true taxon_notes 分类_注释 taxon_notes 分类_注释 分类_注释 Notes on the how instances of this class vary across species. 关于这个类的实例在不同物种间的差异的注释。 UBPROP:0000009 uberon function_notes 功能_注释 true function_notes 功能_注释 function_notes 功能_注释 功能_注释 Notes on the evolved function of instances of this class. 关于这个类的实例的演变函数的注释。 This annotation property may be replaced with an annotation property from an external ontology such as IAO 这个注解属性可以用来自外部本体的注释属性（例如IAO）替换 UBPROP:0000010 uberon structure_notes 结构_注释 true structure_notes 结构_注释 structure_notes 结构_注释 结构_注释 Notes on the structure, composition or histology of instances of this class. 关于这个类的实例的结构、组成或组织学的注释。 This annotation property may be replaced with an annotation property from an external ontology such as IAO 这个注解属性可以用来自外部本体的注释属性（例如IAO）替换 UBPROP:0000011 uberon development_notes 发育_注释 true development_notes 发育_注释 development_notes 发育_注释 发育_注释 Notes on the ontogenic development of instances of this class. 关于这个类的实例的个体发育的注释。 This annotation property may be replaced with an annotation property from an external ontology such as IAO 这个注解属性可以用来自外部本体的注释属性（例如IAO）替换 UBPROP:0000012 uberon external_ontology_notes 外部_本体_注释 true external_ontology_notes 外部_本体_注释 external_ontology_notes 外部_本体_注释 外部_本体_注释 Notes on how similar or equivalent classes are represented in other ontologies. 注意在其他本体中如何表示相似或等价的类。 This annotation property may be replaced with an annotation property from an external ontology such as IAO 这个注解属性可以用来自外部本体的注释属性（例如IAO）替换 UBPROP:0000202 uberon fma_set_term fma_设定_术语 true fma_set_term fma_设定_术语 fma_set_term fma_设定_术语 fma_设定_术语 FMA has terms like 'set of X'. In general we do not include set-of terms in uberon, but provide a mapping between the singular form and the FMA set term FMA具有像'一组X'这样的术语。一般来说，我们不包含uberon中的集合项，但提供单数形式和FMA集合项之间的映射 A metadata relation between a class and its taxonomic rank (eg species, family) ncbi_taxonomy ncbi_taxonomy has_rank has_rank Typically, a Title will be a name by which the resource is formally known. A name given to the resource. Title Title uberon dc-contributor dc-贡献者 true dc-contributor dc-贡献者 Examples of a Contributor include a person, an organisation, or a service. Typically, the name of a Contributor should be used to indicate the entity. 贡献者的例子包括一个人，一个 组织或者一个服务。 典型地， 一个贡献者的名字 应该用于指示这个实体. An entity responsible for making contributions to the content of the resource. 一个实体应该有助于 资源的内容 Contributor Contributor contributor 贡献者 贡献者 Examples of a Creator include a person, an organisation, or a service. Typically, the name of a Creator should be used to indicate the entity. An entity primarily responsible for making the content of the resource. Creator Creator Typically, Date will be associated with the creation or availability of the resource. Recommended best practice for encoding the date value is defined in a profile of ISO 8601 [W3CDTF] and follows the YYYY-MM-DD format. A date associated with an event in the life cycle of the resource. Date Date Description may include but is not limited to: an abstract, table of contents, reference to a graphical representation of content or a free-text account of the content. 描述应该包含但不限于一个摘要， 内容表格, 内容的图形表示 或内容的自由文本描述 An account of the content of the resource. 资源内容的说明。 Description Description 描述 描述 Typically, Format may include the media-type or dimensions of the resource. Format may be used to determine the software, hardware or other equipment needed to display or operate the resource. Examples of dimensions include size and duration. Recommended best practice is to select a value from a controlled vocabulary (for example, the list of Internet Media Types [MIME] defining computer media formats). The physical or digital manifestation of the resource. Format Format Typically, a Subject will be expressed as keywords, key phrases or classification codes that describe a topic of the resource. Recommended best practice is to select a value from a controlled vocabulary or formal classification scheme. The topic of the content of the resource. Subject and Keywords Subject and Keywords has_alternative_id 有_可替代的_id 有_可替代的_id has_broad_synonym 有_广义的_同义词 有_广义的_同义词 database_cross_reference database_cross_reference 数据库_交叉_引用 数据库_交叉_引用 has_exact_synonym 有_精确的_同义词 有_精确的_同义词 has_narrow_synonym 有_狭义的_同义词 有_狭义的_同义词 has_obo_namespace has_obo_namespace 有_obo_命名空间 有_obo_命名空间 has_related_synonym 有_相关的_同义词 有_相关的_同义词 in_subset 在_子集 在_子集 shorthand shorthand label label label 标签 depicted_by 由_描述 part of part_of has part has_part inheres in at all times realizes concretized by at some time occurs in located_in occupies spatial region at some time has_quality exists at has occurrent part has spatial occupant at some time part of occurrent occupies temporal region has temporal occupant has disposition at all times specifically depends on at some time part of continuant at some time has continuant part at some time part of continuant at all times that whole exists has continuant part at all times that part exists derives from patient having disease has disease is in cell line repository is disease model for derived from anatomic part induced by cell culture reagent overexpresses protein overexpresses gene is about has_specified_input is_specified_input_of has_specified_output is_specified_output_of achieves_planned_objective has grain is genome of organism is gene of organism bearer of participates in has participant concretizes has_quality has role derives from derives_from has plasma membrane part only_in_taxon develops from develops into capable of p has output c iff c is a participant in p, c is present at the end of p, and c is not present at the beginning of p. Chris Mungall produces has output Inverse of 'expressed in' Chris Mungall expresses output_of existence starts during a produces b if some process that occurs_in a has_output b, where a and b are material entities. Examples: hybridoma cell line produces monoclonal antibody reagent; chondroblast produces avascular GAG-rich matrix. Melissa Haendel Note that this definition doesn't quite distinguish the output of a transformation process from a production process, which is related to the identity/granularity issue. produces has_high_plasma_membrane_amount has_low_plasma_membrane_amount lacks_part lacks_plasma_membrane_part has_gene_template lacks_part has expression has_disease_location peripheral vein 外周静脉 SNOMEDID: T-48097 URI: http://www.ebi.ac.uk/cellline#Structure_of_peripheral_vein Gonadal Gonads A reproductive system organ that produces and releases either sperm (testis) or eggs (ovary). The part of the reproductive system that produces and releases eggs (ovaries) or sperm (testicles/testes).NCI-GLOSS 产生和释放卵子（卵巢）或精子（睾丸）的生殖系统的一部分.NCI-GLOSS 产生和释放精子（睾丸）或卵子（卵巢）的生殖系统器官。 gonad 性腺 UMLS: C0018067 URI: http://www.ebi.ac.uk/cellline#Organ entity entity 实体 Entity Entity 实体 entity 实体 Julius Caesar Verdi’s Requiem the Second World War your body mass index 你的身体质量指数 凯撒大帝 威尔第年代安魂曲 第二次世界大战 BFO 2 Reference: In all areas of empirical inquiry we encounter general terms of two sorts. First are general terms which refer to universals or types:animaltuberculosissurgical procedurediseaseSecond, are general terms used to refer to groups of entities which instantiate a given universal but do not correspond to the extension of any subuniversal of that universal because there is nothing intrinsic to the entities in question by virtue of which they – and only they – are counted as belonging to the given group. Examples are: animal purchased by the Emperortuberculosis diagnosed on a Wednesdaysurgical procedure performed on a patient from Stockholmperson identified as candidate for clinical trial #2056-555person who is signatory of Form 656-PPVpainting by Leonardo da VinciSuch terms, which represent what are called ‘specializations’ in [81 BFO 2参考：在经验性总结分析的所有领域，我们遇到两种一般术语。首先是指共相或类型的一般术语：animaltuberculosissurgical proceduredisease其次，是用来指以下实例组的一般术语，这些实例组实例化一个给定共性但对应于该共相的任何子共相的扩展，因为没有实体的内在本质讨论因为他们-只有他们-被视为属于给定的群体。例子是：对斯德哥尔摩病人进行的周三手术过程中诊断出结核分枝杆菌而购买的动物，被确定为临床试验的候选人＃2056-555根据莱昂纳多·达·芬奇（Leonardo da Vinci）条款制定的656-PPV表格的签名人。这些术语代表所谓的“专业化'[81 Entity doesn't have a closure axiom because the subclasses don't necessarily exhaust all possibilites. For example Werner Ceusters 'portions of reality' include 4 sorts, entities (as BFO construes them), universals, configurations, and relations. It is an open question as to whether entities as construed in BFO will at some point also include these other portions of reality. See, for example, 'How to track absolutely everything' at http://www.referent-tracking.com/_RTU/papers/CeustersICbookRevised.pdf 实体不具有闭合公理，因为子类不一定丧失所有可能性。例如，Werner Ceusters的“现实的部分”包括4类，实体（当BFO构造它们时）、普遍性、配置和关系。这是一个开放性的问题，即在BFO中所解释的实体是否会在某个时候也包含这些其他的现实部分。参见，例如“如何完全追踪所有事物”在http://www.referent-tracking.com/_RTU/papers/CeustersICbookRevised.pdf An entity is anything that exists or has existed or will exist. (axiom label in BFO2 Reference: [001-001]) 一个实体是存在或已经存在或将存在的任何事物。 （BFO2公理标签参考：[001-001]） entity 实体 continuant continuant 常体 Continuant Continuant 常体 continuant 常体 An entity that exists in full at any time in which it exists at all, persists through time while maintaining its identity and has no temporal parts. 一个在任何时候都存在的实体，它持续存在，在维持其特性的同时且没有暂时的部分。 BFO 2 Reference: Continuant entities are entities which can be sliced to yield parts only along the spatial dimension, yielding for example the parts of your table which we call its legs, its top, its nails. ‘My desk stretches from the window to the door. It has spatial parts, and can be sliced (in space) in two. With respect to time, however, a thing is a continuant.’ [60, p. 240 BFO 2参考：持续实体是可以被分割的实体，只能沿着空间维度分割成部分，例如产生您的桌子的部分，我们称之为它的腿、顶部、指甲。 “我的桌子从窗户延伸到门口。它有空间部分，可以在空间切成两半。不过，就时间而言，一个事物是连续的。“[60，p。 240 Continuant doesn't have a closure axiom because the subclasses don't necessarily exhaust all possibilites. For example, in an expansion involving bringing in some of Ceuster's other portions of reality, questions are raised as to whether universals are continuants 常体不具有闭合公理，因为子类不一定丧失所有可能性。例如，在涉及引入一些Ceuster的实体的其他部分的扩展中，提出了普遍性是否是连续性的问题 A continuant is an entity that persists, endures, or continues to exist through time while maintaining its identity. (axiom label in BFO2 Reference: [008-002]) 一个常体是一个持续、持久或持续存在且同时保持其身份的实体。 （BFO2中的公理标签参考：[008-002]） if b is a continuant and if, for some t, c has_continuant_part b at t, then c is a continuant. (axiom label in BFO2 Reference: [126-001]) if b is a continuant and if, for some t, cis continuant_part of b at t, then c is a continuant. (axiom label in BFO2 Reference: [009-002]) if b is a material entity, then there is some temporal interval (referred to below as a one-dimensional temporal region) during which b exists. (axiom label in BFO2 Reference: [011-002]) 如果b是一个常体，并且如果对于某个t，c在t时刻具有_常体_部分b，则c是一个常体。 （BFO2公理标签参考：[126-001]） 如果b是一个常体，并且如果，对于某个t，c是在t时刻的b的常体_部分，则c是一个常体。 （BFO2公理标签参考：[009-002]） 如果b是物质实体，则在b存在期间有一些时间间隔（以下称为一维时间区域）。 （BFO2公理标签：[011-002]） (forall (x y) (if (and (Continuant x) (exists (t) (continuantPartOfAt y x t))) (Continuant y))) // axiom label in BFO2 CLIF: [009-002] (forall (x y) (if (and (Continuant x) (exists (t) (continuantPartOfAt y x t))) (Continuant y))) // axiom label in BFO2 CLIF: [009-002] (forall (x y) (if (and (Continuant x) (exists (t) (hasContinuantPartOfAt y x t))) (Continuant y))) // axiom label in BFO2 CLIF: [126-001] (forall (x y) (if (and (Continuant x) (exists (t) (hasContinuantPartOfAt y x t))) (Continuant y))) // axiom label in BFO2 CLIF: [126-001] (forall (x) (if (Continuant x) (Entity x))) // axiom label in BFO2 CLIF: [008-002] (forall (x) (if (Continuant x) (Entity x))) // axiom label in BFO2 CLIF: [008-002] (forall (x) (if (Material Entity x) (exists (t) (and (TemporalRegion t) (existsAt x t))))) // axiom label in BFO2 CLIF: [011-002] (forall (x) (if (Material Entity x) (exists (t) (and (TemporalRegion t) (existsAt x t))))) // axiom label in BFO2 CLIF: [011-002] continuant 常体 occurrent occurrent 行体 Occurrent Occurrent 行体 occurrent 行体 An entity that has temporal parts and that happens, unfolds or develops through time. 具有时间部分且随时间发生、展开或发展的实体。 BFO 2 Reference: every occurrent that is not a temporal or spatiotemporal region is s-dependent on some independent continuant that is not a spatial region BFO 2 Reference: s-dependence obtains between every process and its participants in the sense that, as a matter of necessity, this process could not have existed unless these or those participants existed also. A process may have a succession of participants at different phases of its unfolding. Thus there may be different players on the field at different times during the course of a football game; but the process which is the entire game s-depends_on all of these players nonetheless. Some temporal parts of this process will s-depend_on on only some of the players. BFO 2参考：不是时间或时空区域的每一个行体都是依赖于某个独立的且不是空间区域的常体 BFO 2参考：每个过程之间获得的s-依赖和它的参与者从某种意义上说，必要的时候，除非这些或那些参与者也存在，否则这个过程是不可能存在的。一个过程可能有一系列参与者在其展开的不同阶段。因此在足球比赛过程中的不同时间场上可能会有不同的球员;但整个游戏的过程依赖于所有这些玩家。这个过程的一些暂时的部分将只s-依赖_于一些玩家。 Occurrent doesn't have a closure axiom because the subclasses don't necessarily exhaust all possibilites. An example would be the sum of a process and the process boundary of another process. Simons uses different terminology for relations of occurrents to regions: Denote the spatio-temporal location of a given occurrent e by 'spn[e]' and call this region its span. We may say an occurrent is at its span, in any larger region, and covers any smaller region. Now suppose we have fixed a frame of reference so that we can speak not merely of spatio-temporal but also of spatial regions (places) and temporal regions (times). The spread of an occurrent, (relative to a frame of reference) is the space it exactly occupies, and its spell is likewise the time it exactly occupies. We write 'spr[e]' and `spl[e]' respectively for the spread and spell of e, omitting mention of the frame. Simons uses different terminology for relations of occurrents to regions: Denote the spatio-temporal location of a given occurrent e by 'spn[e]' and call this region its span. We may say an occurrent is at its span, in any larger region, and covers any smaller region. Now suppose we have fixed a frame of reference so that we can speak not merely of spatio-temporal but also of spatial regions (places) and temporal regions (times). The spread of an occurrent, (relative to a frame of reference) is the space it exactly occupies, and its spell is likewise the time it exactly occupies. We write 'spr[e]' and `spl[e]' respectively for the spread and spell of e, omitting mention of the frame. Simons使用不同的术语来表示发生在不同区域的行体关系：通过'spn [e]'表示给定occurrent e的时空位置。并称这个区域为跨度。我们可以说一个行体在它的跨度内，在任何一个较大的地区，覆盖了所有较小的地区。现在假设我们已经固定了一个参考框架，以便我们不仅可以谈及时空，而且可以谈及空间区域（地点）和时间区域（时间）。一个行体的（相对于一个参考框架）的延伸是它正好占据的空间，而它的拼写同样也是它正好占据的时间。我们写“spr [e]”和“spl [e]”。分别为e的延伸和拼写，省略框架的提及。 行体并不具有闭合公理，因为子类不一定丧失所有可能性。一个例子是一个进程和另一个进程的进程边界的总和。 An occurrent is an entity that unfolds itself in time or it is the instantaneous boundary of such an entity (for example a beginning or an ending) or it is a temporal or spatiotemporal region which such an entity occupies_temporal_region or occupies_spatiotemporal_region. (axiom label in BFO2 Reference: [077-002]) 一个行体是一个在时间上展开的实体，或者是一个实体的瞬时边界（例如一个起点或终点），或者是一个时空或时空区域，其中这样一个实体占据_时空_区域或占据_空间_区域的。 （BFO2公理标签参考：[077-002]） Every occurrent occupies_spatiotemporal_region some spatiotemporal region. (axiom label in BFO2 Reference: [108-001]) b is an occurrent entity iff b is an entity that has temporal parts. (axiom label in BFO2 Reference: [079-001]) 如果b是具有时间部分的实体，则b是一个行体实体。 （BFO2公理标签参考：[079-001]） 每一个行体都会占据_时空_区域一些时空区域。 （BFO2公理标签参考：[108-001]） (forall (x) (if (Occurrent x) (exists (r) (and (SpatioTemporalRegion r) (occupiesSpatioTemporalRegion x r))))) // axiom label in BFO2 CLIF: [108-001] (forall (x) (if (Occurrent x) (exists (r) (and (SpatioTemporalRegion r) (occupiesSpatioTemporalRegion x r))))) // axiom label in BFO2 CLIF: [108-001] (forall (x) (iff (Occurrent x) (and (Entity x) (exists (y) (temporalPartOf y x))))) // axiom label in BFO2 CLIF: [079-001] (forall (x) (iff (Occurrent x) (and (Entity x) (exists (y) (temporalPartOf y x))))) // axiom label in BFO2 CLIF: [079-001] occurrent 行体 ic ic IndependentContinuant IndependentContinuant 独立常体(IndependentContinuant) independent continuant 独立常体(Independent Continuant) a chair a heart a leg a molecule a spatial region an atom an orchestra. an organism the bottom right portion of a human torso the interior of your mouth 一个分子 一个原子 一个心脏 一个有机体 一个空间区域 一个管弦乐队。 一把椅子 一条腿 人体躯干的右下部分 你的嘴巴内部 A continuant that is a bearer of quality and realizable entity entities, in which other entities inhere and which itself cannot inhere in anything. b is an independent continuant = Def. b is a continuant which is such that there is no c and no t such that b s-depends_on c at t. (axiom label in BFO2 Reference: [017-002]) b是一个独立常体= Def. b是一个常体，使得在t时刻不存在c，也不存在t，从而在t时刻b s-依赖_于c。 （BFO2公理标签参考：[017-002]） 常体是质量和可实现实体的载体，其中其他实体在其中并且本身不能在任何地方存在。 For any independent continuant b and any time t there is some spatial region r such that b is located_in r at t. (axiom label in BFO2 Reference: [134-001]) For every independent continuant b and time t during the region of time spanned by its life, there are entities which s-depends_on b during t. (axiom label in BFO2 Reference: [018-002]) 对于任何独立常体b和任何时刻t，都有一些空间区域r，使得b在t时刻位_于r处。 （BFO2公理标签参考：[134-001]） 对于在其生命周期中的每个时间区域内每个独立的常体b和时间t，在t期间存在s-依赖_于b的实体。 （BFO2公理标签参考：[018-002]） (forall (x t) (if (IndependentContinuant x) (exists (r) (and (SpatialRegion r) (locatedInAt x r t))))) // axiom label in BFO2 CLIF: [134-001] (forall (x t) (if (IndependentContinuant x) (exists (r) (and (SpatialRegion r) (locatedInAt x r t))))) // axiom label in BFO2 CLIF: [134-001] (forall (x t) (if (and (IndependentContinuant x) (existsAt x t)) (exists (y) (and (Entity y) (specificallyDependsOnAt y x t))))) // axiom label in BFO2 CLIF: [018-002] (forall (x t) (if (and (IndependentContinuant x) (existsAt x t)) (exists (y) (and (Entity y) (specificallyDependsOnAt y x t))))) // axiom label in BFO2 CLIF: [018-002] (iff (IndependentContinuant a) (and (Continuant a) (not (exists (b t) (specificallyDependsOnAt a b t))))) // axiom label in BFO2 CLIF: [017-002] (iff (IndependentContinuant a) (and (Continuant a) (not (exists (b t) (specificallyDependsOnAt a b t))))) // axiom label in BFO2 CLIF: [017-002] independent continuant 独立常体 true true s-region SpatialRegion BFO 2 Reference: Spatial regions do not participate in processes. Spatial region doesn't have a closure axiom because the subclasses don't exhaust all possibilites. An example would be the union of a spatial point and a spatial line that doesn't overlap the point, or two spatial lines that intersect at a single point. In both cases the resultant spatial region is neither 0-dimensional, 1-dimensional, 2-dimensional, or 3-dimensional. A spatial region is a continuant entity that is a continuant_part_of spaceR as defined relative to some frame R. (axiom label in BFO2 Reference: [035-001]) All continuant parts of spatial regions are spatial regions. (axiom label in BFO2 Reference: [036-001]) (forall (x y t) (if (and (SpatialRegion x) (continuantPartOfAt y x t)) (SpatialRegion y))) // axiom label in BFO2 CLIF: [036-001] (forall (x) (if (SpatialRegion x) (Continuant x))) // axiom label in BFO2 CLIF: [035-001] spatial region true true t-region TemporalRegion Temporal region doesn't have a closure axiom because the subclasses don't exhaust all possibilites. An example would be the mereological sum of a temporal instant and a temporal interval that doesn't overlap the instant. In this case the resultant temporal region is neither 0-dimensional nor 1-dimensional A temporal region is an occurrent entity that is part of time as defined relative to some reference frame. (axiom label in BFO2 Reference: [100-001]) All parts of temporal regions are temporal regions. (axiom label in BFO2 Reference: [101-001]) Every temporal region t is such that t occupies_temporal_region t. (axiom label in BFO2 Reference: [119-002]) (forall (r) (if (TemporalRegion r) (occupiesTemporalRegion r r))) // axiom label in BFO2 CLIF: [119-002] (forall (x y) (if (and (TemporalRegion x) (occurrentPartOf y x)) (TemporalRegion y))) // axiom label in BFO2 CLIF: [101-001] (forall (x) (if (TemporalRegion x) (Occurrent x))) // axiom label in BFO2 CLIF: [100-001] temporal region 时间区域 process 过程 Process 过程 process 过程 a process of cell-division, \ a beating of the heart a process of meiosis a process of sleeping the course of a disease the flight of a bird the life of an organism your process of aging. 一个减数分裂的过程 一个生物体的生命 一个睡眠的过程 一个细胞分裂的过程，\一个跳动的心脏 一只鸟的飞行 你的老化过程。 疾病的过程 An occurrent that has temporal proper parts and for some time t, p s-depends_on some material entity at t. p is a process = Def. p is an occurrent that has temporal proper parts and for some time t, p s-depends_on some material entity at t. (axiom label in BFO2 Reference: [083-003]) p是一个进程= Def. p是一个常体，其具有时间适当的部分且对于某个时间t，p s-依赖_于t时刻的一些物质实体。 （BFO2公理标签参考：[083-003]） 一个常体，其具有时间适当的部分且对于某个时间t，p s-依赖_于t时刻的一些物质实体。 BFO 2 Reference: The realm of occurrents is less pervasively marked by the presence of natural units than is the case in the realm of independent continuants. Thus there is here no counterpart of ‘object’. In BFO 1.0 ‘process’ served as such a counterpart. In BFO 2.0 ‘process’ is, rather, the occurrent counterpart of ‘material entity’. Those natural – as contrasted with engineered, which here means: deliberately executed – units which do exist in the realm of occurrents are typically either parasitic on the existence of natural units on the continuant side, or they are fiat in nature. Thus we can count lives; we can count football games; we can count chemical reactions performed in experiments or in chemical manufacturing. We cannot count the processes taking place, for instance, in an episode of insect mating behavior.Even where natural units are identifiable, for example cycles in a cyclical process such as the beating of a heart or an organism’s sleep/wake cycle, the processes in question form a sequence with no discontinuities (temporal gaps) of the sort that we find for instance where billiard balls or zebrafish or planets are separated by clear spatial gaps. Lives of organisms are process units, but they too unfold in a continuous series from other, prior processes such as fertilization, and they unfold in turn in continuous series of post-life processes such as post-mortem decay. Clear examples of boundaries of processes are almost always of the fiat sort (midnight, a time of death as declared in an operating theater or on a death certificate, the initiation of a state of war) BFO 2参考文献：相比在行体领域的情况，自然单位在独立常体领域的存在更普遍。因此这里没有对象的对等物。在BFO 1.0'过程'中充当了这样一个对等物。在BFO 2.0中，“过程”相当于“物质实体”的对等物。那些自然的-与工程设计相对比，这里的意思是：在现实领域中确实存在的故意执行-单位通常是寄生于常体侧存在的自然单位，或者它们本质上是允许的。因此，我们可以计算生命;我们可以算足球比赛;我们可以计算在实验或化学制造中进行的化学反应。我们不能计算如发生在昆虫交配行为中的过程。即使自然单位是可识别的，例如周期性过程的周期，例如跳动心脏或生物体的睡眠/觉醒周期，过程在讨论中形成一个没有我们发现的那种不连续性（时间间隔）的序列，在这种不连续性中，如台球或斑马鱼或行星被明确的空间差距分开。有机体的生命是过程单元，但是它们也是从其他先前的过程（如受精）中连续地展开，然后展开连续的一系列后期过程，例如死后衰变。过程界限的清晰例子几乎总是属于法定类型（午夜，手术室宣布的死亡时间或者死亡证明，战争状态的开始） (iff (Process a) (and (Occurrent a) (exists (b) (properTemporalPartOf b a)) (exists (c t) (and (MaterialEntity c) (specificallyDependsOnAt a c t))))) // axiom label in BFO2 CLIF: [083-003] (iff (Process a) (and (Occurrent a) (exists (b) (properTemporalPartOf b a)) (exists (c t) (and (MaterialEntity c) (specificallyDependsOnAt a c t))))) // axiom label in BFO2 CLIF: [083-003] bfo BFO:0000015 process 过程 disposition disposition 倾向 Disposition Disposition 倾向 disposition 倾向 an atom of element X has the disposition to decay to an atom of element Y certain people have a predisposition to colon cancer children are innately disposed to categorize objects in certain ways. the cell wall is disposed to filter chemicals in endocitosis and exocitosis the cell wall is disposed to filter chemicals in endocytosis and exocytosis 元素X的原子具有衰减到元素Y的原子的倾向 孩子天生就倾向于以特定的方式对物体进行分类。 某些人有结肠癌的倾向 细胞壁被设置为过滤内吞作用和胞吐作用中的化学物质 BFO 2 Reference: Dispositions exist along a strength continuum. Weaker forms of disposition are realized in only a fraction of triggering cases. These forms occur in a significant number of cases of a similar type [89 BFO 2 Reference: Dispositions exist along a strength continuum. Weaker forms of disposition are realized in only a fraction of triggering cases. These forms occur in a significant number of cases of a similar type. BFO 2参考：倾向存在于强度连续体中。只有一小部分触发事件才能实现较弱的倾向形式。这些形式出现在相当数量的类似案例中。 b is a disposition means: b is a realizable entity & b’s bearer is some material entity & b is such that if it ceases to exist, then its bearer is physically changed, & b’s realization occurs when and because this bearer is in some special physical circumstances, & this realization occurs in virtue of the bearer’s physical make-up. (axiom label in BFO2 Reference: [062-002]) b是倾向方式：b是可实现的实体； b的持有人是一些物质实体； b是这样的，如果它不复存在，那么它的持有人发生了物理变化； b的实现发生在且因为这个持有者处于一些特殊的物理环境中；这种实现是由于持有者的物理构成而发生的。 （BFO2公理标签参考：[062-002]） If b is a realizable entity then for all t at which b exists, b s-depends_on some material entity at t. (axiom label in BFO2 Reference: [063-002]) 如果b是一个可实现的实体，那么对于存在b的所有t时刻，b s-依赖_于t时刻的一个物质实体。 （BFO2公理标签参考：[063-002]） (forall (x t) (if (and (RealizableEntity x) (existsAt x t)) (exists (y) (and (MaterialEntity y) (specificallyDepends x y t))))) // axiom label in BFO2 CLIF: [063-002] (forall (x t) (if (and (RealizableEntity x) (existsAt x t)) (exists (y) (and (MaterialEntity y) (specificallyDepends x y t))))) // axiom label in BFO2 CLIF: [063-002] (forall (x) (if (Disposition x) (and (RealizableEntity x) (exists (y) (and (MaterialEntity y) (bearerOfAt x y t)))))) // axiom label in BFO2 CLIF: [062-002] (forall (x) (if (Disposition x) (and (RealizableEntity x) (exists (y) (and (MaterialEntity y) (bearerOfAt x y t)))))) // axiom label in BFO2 CLIF: [062-002] disposition 倾向 realizable realizable 可实现的 RealizableEntity RealizableEntity 可实现实体(RealizableEntity) realizable entity 可实现实体 the disposition of this piece of metal to conduct electricity. the disposition of your blood to coagulate the function of your reproductive organs the role of being a doctor the role of this boundary to delineate where Utah and Colorado meet 作为医生的角色 你的生殖器官的功能 你的血液的凝结倾向 这个边界角色描绘了犹他州和科罗拉多州的交汇点 这块金属的导电倾向。 A specifically dependent continuant that inheres in continuant entities and are not exhibited in full at every time in which it inheres in an entity or group of entities. The exhibition or actualization of a realizable entity is a particular manifestation, functioning or process that occurs under certain circumstances. 一个特定依赖性常体，在常体实体中存在，并且在每个实体或一组实体中都不会全部展现出来。可实现实体的展示或实现是在特定情况下发生的特定表现、功能或过程。 To say that b is a realizable entity is to say that b is a specifically dependent continuant that inheres in some independent continuant which is not a spatial region and is of a type instances of which are realized in processes of a correlated type. (axiom label in BFO2 Reference: [058-002]) 要说b是一个可实现实体，就是说b是一个特定依赖性常体，它存在于某些依赖常体中，这个连续体不是一个空间区域，而是一个在相关类型的过程中实现的类型实例。 （BFO2公理标签参考：[058-002]） All realizable dependent continuants have independent continuants that are not spatial regions as their bearers. (axiom label in BFO2 Reference: [060-002]) 所有可实现依赖常体都有独立常体，这些独立常体不是空间区域作为承载。 （BFO2公理标签参考：[060-002]） (forall (x t) (if (RealizableEntity x) (exists (y) (and (IndependentContinuant y) (not (SpatialRegion y)) (bearerOfAt y x t))))) // axiom label in BFO2 CLIF: [060-002] (forall (x t) (if (RealizableEntity x) (exists (y) (and (IndependentContinuant y) (not (SpatialRegion y)) (bearerOfAt y x t))))) // axiom label in BFO2 CLIF: [060-002] (forall (x) (if (RealizableEntity x) (and (SpecificallyDependentContinuant x) (exists (y) (and (IndependentContinuant y) (not (SpatialRegion y)) (inheresIn x y)))))) // axiom label in BFO2 CLIF: [058-002] (forall (x) (if (RealizableEntity x) (and (SpecificallyDependentContinuant x) (exists (y) (and (IndependentContinuant y) (not (SpatialRegion y)) (inheresIn x y)))))) // axiom label in BFO2 CLIF: [058-002] realizable entity 可实现实体 quality quality 性质 Quality Quality 性质 quality 性质 the ambient temperature of this portion of air the color of a tomato the length of the circumference of your waist the mass of this piece of gold. the shape of your nose the shape of your nostril 你的腰围的长度 你的鼻子的形状 你的鼻孔的形状 番茄的颜色 这块黄金的质量。 这部分空气的环境温度 a quality is a specifically dependent continuant that, in contrast to roles and dispositions, does not require any further process in order to be realized. (axiom label in BFO2 Reference: [055-001]) 质量是一个特定依赖性常体，与角色和性格相反，不需要任何进一步的过程才能实现。 （BFO2公理标签参考：[055-001]） If an entity is a quality at any time that it exists, then it is a quality at every time that it exists. (axiom label in BFO2 Reference: [105-001]) 如果一个实体在任何时候都存在一个性质，那么在它存在的每个时间它就是一个性质。 （BFO2公理标签参考：[105-001]） (forall (x) (if (Quality x) (SpecificallyDependentContinuant x))) // axiom label in BFO2 CLIF: [055-001] (forall (x) (if (Quality x) (SpecificallyDependentContinuant x))) // axiom label in BFO2 CLIF: [055-001] (forall (x) (if (exists (t) (and (existsAt x t) (Quality x))) (forall (t_1) (if (existsAt x t_1) (Quality x))))) // axiom label in BFO2 CLIF: [105-001] (forall (x) (if (exists (t) (and (existsAt x t) (Quality x))) (forall (t_1) (if (existsAt x t_1) (Quality x))))) // axiom label in BFO2 CLIF: [105-001] bfo BFO:0000019 quality 性质 sdc sdc SpecificallyDependentContinuant SpecificallyDependentContinuant 特定依赖性常体(SpecificallyDependentContinuant) specifically dependent continuant 特定依赖性常体 Reciprocal specifically dependent continuants: the function of this key to open this lock and the mutually dependent disposition of this lock: to be opened by this key of one-sided specifically dependent continuants: the mass of this tomato of relational dependent continuants (multiple bearers): John’s love for Mary, the ownership relation between John and this statue, the relation of authority between John and his subordinates. the disposition of this fish to decay the function of this heart: to pump blood the mutual dependence of proton donors and acceptors in chemical reactions [79 the mutual dependence of the role predator and the role prey as played by two organisms in a given interaction the pink color of a medium rare piece of grilled filet mignon at its center the role of being a doctor the shape of this hole. the smell of this portion of mozzarella 交互特定依赖性常体：这个键的功能打开这个锁和这个锁的相互依赖的配置：被这个键打开 作为医生的角色 关系独立常体（对个载体）：约翰对玛丽的爱，约翰与这座雕像之间的所有关系，以及约翰与他的下属之间的权力关系。 在它中心的一块四分熟烤菲力牛排的粉色 局部特定依赖性常体：这个番茄的性质 捕食者角色和猎物角色的相互依赖由两个生物体在给定的相互作用中扮演 质子供体和受体在化学反应中的相互依赖[79 这个洞的形状。 这条鱼的腐烂倾向 这部分马苏里拉奶酪的气味 这颗心的功能：抽血 A continuant that inheres in or is borne by other entities. Every instance of A requires some specific instance of B which must always be the same. b is a relational specifically dependent continuant = Def. b is a specifically dependent continuant and there are n &gt; 1 independent continuants c1, … cn which are not spatial regions are such that for all 1 i &lt; j n, ci and cj share no common parts, are such that for each 1 i n, b s-depends_on ci at every time t during the course of b’s existence (axiom label in BFO2 Reference: [131-004]) b is a specifically dependent continuant = Def. b is a continuant & there is some independent continuant c which is not a spatial region and which is such that b s-depends_on c at every time t during the course of b’s existence. (axiom label in BFO2 Reference: [050-003]) b是一个关系特定依赖性常体 = Def. b是特定依赖性常体并且有n &gt;1个独立常体c1，... cn不是空间区域且是这样的，即对于全部1个 i&lt;j n，ci和cj没有共同的部分，对于每个1 i n，在b存在的过程中每个时间t，b s-依赖_于 ci（BFO2中的公理标号：[131-004]） b是一个特定依赖性常体 = Def. b是一个常体& 存在一些独立常体c，c不是一个空间区域且是这样的，在b的存在过程地每个时间t，b s-依赖_于c。 （BFO2公理标签参考：[050-003]） 一个在其他实体中存在或由其他实体承担的常体。 A的每个实例都需要一些特定的B实例，这些B实例必须始终相同。 Specifically dependent continuant doesn't have a closure axiom because the subclasses don't necessarily exhaust all possibilites. We're not sure what else will develop here, but for example there are questions such as what are promises, obligation, etc. 具体而言，相关常体不具有闭合公理，因为子类不一定丧失所有可能性。我们不确定在这里会发生什么，但是会有如什么是承诺，义务等等的问题。 (iff (RelationalSpecificallyDependentContinuant a) (and (SpecificallyDependentContinuant a) (forall (t) (exists (b c) (and (not (SpatialRegion b)) (not (SpatialRegion c)) (not (= b c)) (not (exists (d) (and (continuantPartOfAt d b t) (continuantPartOfAt d c t)))) (specificallyDependsOnAt a b t) (specificallyDependsOnAt a c t)))))) // axiom label in BFO2 CLIF: [131-004] (iff (RelationalSpecificallyDependentContinuant a) (and (SpecificallyDependentContinuant a) (forall (t) (exists (b c) (and (not (SpatialRegion b)) (not (SpatialRegion c)) (not (= b c)) (not (exists (d) (and (continuantPartOfAt d b t) (continuantPartOfAt d c t)))) (specificallyDependsOnAt a b t) (specificallyDependsOnAt a c t)))))) // axiom label in BFO2 CLIF: [131-004] (iff (SpecificallyDependentContinuant a) (and (Continuant a) (forall (t) (if (existsAt a t) (exists (b) (and (IndependentContinuant b) (not (SpatialRegion b)) (specificallyDependsOnAt a b t))))))) // axiom label in BFO2 CLIF: [050-003] (iff (SpecificallyDependentContinuant a) (and (Continuant a) (forall (t) (if (existsAt a t) (exists (b) (and (IndependentContinuant b) (not (SpatialRegion b)) (specificallyDependsOnAt a b t))))))) // axiom label in BFO2 CLIF: [050-003] specifically dependent continuant 特定依赖性常体 role role 角色 Role Role 角色 role 角色 John’s role of husband to Mary is dependent on Mary’s role of wife to John, and both are dependent on the object aggregate comprising John and Mary as member parts joined together through the relational quality of being married. the priest role the role of a boundary to demarcate two neighboring administrative territories the role of a building in serving as a military target the role of a stone in marking a property boundary the role of subject in a clinical trial the student role 作为军事目标的建筑物的角色 学生角色 患者在临床试验中的作用 牧师的角色 石头在标记一个属性边界中的角色 约翰对玛丽的丈夫角色依赖于玛丽对约翰的妻子角色，二者都依赖于由约翰和玛丽组成的对象集合，其中包括约翰和玛丽作为成员部分通过婚姻关系性质而成为一体。 边界划分两个邻近的行政区域的角色 A realizable entity the manifestation of which brings about some result or end that is not essential to a continuant in virtue of the kind of thing that it is but that can be served or participated in by that kind of continuant in some kinds of natural, social or institutional contexts. 一个可实现实体，它的表现会带来某种结果或结局，而这种结果或结局对于一个常体来说并不是必不可少的，因为它是一种事物，但它可以在某种自然的，社会的或制度的环境中，被这种常体所服务或参与。 BFO 2 Reference: One major family of examples of non-rigid universals involves roles, and ontologies developed for corresponding administrative purposes may consist entirely of representatives of entities of this sort. Thus ‘professor’, defined as follows,b instance_of professor at t =Def. there is some c, c instance_of professor role & c inheres_in b at t.denotes a non-rigid universal and so also do ‘nurse’, ‘student’, ‘colonel’, ‘taxpayer’, and so forth. (These terms are all, in the jargon of philosophy, phase sortals.) By using role terms in definitions, we can create a BFO conformant treatment of such entities drawing on the fact that, while an instance of professor may be simultaneously an instance of trade union member, no instance of the type professor role is also (at any time) an instance of the type trade union member role (any more than any instance of the type color is at any time an instance of the type length).If an ontology of employment positions should be defined in terms of roles following the above pattern, this enables the ontology to do justice to the fact that individuals instantiate the corresponding universals – professor, sergeant, nurse – only during certain phases in their lives. BFO 2参考：非刚性共相的一个主要例子涉及角色，及为相应的管理目的而开发的本体可以完全由这类实体的代表组成。因此，“教授”的定义如下，b为t时的教授实例=Def.有一些c，c为教授角色的实例 & 在t时刻，c存在_于b，表示非刚性共相，所以还要做“护士”、“学生”、“上校”、“纳税人”等等。 （这些术语在哲学术语中都是阶段排序的。）通过在定义中使用角色术语，我们可以为这些实体创建一个BFO一致性处理，而这些实体根据这个事实，即一个教授实例可能同时是工会成员，没有教授角色类型的实例也是（在任何时候）工会成员角色类型的实例（任何时候任何颜色类型的实例都是长度类型的实例）。如果就业岗位的本体应该根据上述模式的角色来定义，这就使得本体能够正确地处理这样一个事实，即个人在生命的某个阶段只实例化相应的共相-教授、中士、护士。 b is a role means: b is a realizable entity & b exists because there is some single bearer that is in some special physical, social, or institutional set of circumstances in which this bearer does not have to be& b is not such that, if it ceases to exist, then the physical make-up of the bearer is thereby changed. (axiom label in BFO2 Reference: [061-001]) b是一个角色意味着：b是可实现实体 & b的存在是因为在某些特定的物理、社会或制度环境中存在一些单一的承载者，在这种环境下，承载者不必是“ b不是这样的，如果它不复存在，那么承载者的物理组成就会改变。 （BFO2公理标签参考：[061-001]） (forall (x) (if (Role x) (RealizableEntity x))) // axiom label in BFO2 CLIF: [061-001] (forall (x) (if (Role x) (RealizableEntity x))) // axiom label in BFO2 CLIF: [061-001] role 角色 bfo BFO:0000024 fiat object part 虚拟对象部分 3d-s-region ThreeDimensionalSpatialRegion a cube-shaped region of space a sphere-shaped region of space, A three-dimensional spatial region is a spatial region that is of three dimensions. (axiom label in BFO2 Reference: [040-001]) (forall (x) (if (ThreeDimensionalSpatialRegion x) (SpatialRegion x))) // axiom label in BFO2 CLIF: [040-001] three-dimensional spatial region bfo BFO:0000030 object 客体 gdc gdc GenericallyDependentContinuant GenericallyDependentContinuant 普遍依赖性常体(GenericallyDependentContinuant) generically dependent continuant 普遍依赖性常体 The entries in your database are patterns instantiated as quality instances in your hard drive. The database itself is an aggregate of such patterns. When you create the database you create a particular instance of the generically dependent continuant type database. Each entry in the database is an instance of the generically dependent continuant type IAO: information content entity. the pdf file on your laptop, the pdf file that is a copy thereof on my laptop the sequence of this protein molecule; the sequence that is a copy thereof in that protein molecule. 在您的笔记本电脑上的PDF文件，这个PDF文件是我的笔记本电脑上的副本 数据库中的条目是在硬盘驱动器中实例化为性质实例的模式。数据库本身就是这些模式的集合。在创建数据库时，您将创建普遍依赖性常体类型数据库的特定实例。数据库中的每个条目都是普遍依赖性常体类型IAO的一个实例：信息内容实体。 这个蛋白质分子的序列;在该蛋白质分子中是其序列的拷贝。 A continuant that is dependent on one or other independent continuant bearers. For every instance of A requires some instance of (an independent continuant type) B but which instance of B serves can change from time to time. b is a generically dependent continuant = Def. b is a continuant that g-depends_on one or more other entities. (axiom label in BFO2 Reference: [074-001]) b是一个普遍依赖性常体 = Def. b是g-依赖_于一个或多个其他实体的常体。 （BFO2公理标签参考：[074-001]） 依赖于一个或另一个独立常体承载者的常体。对于A的每个实例都需要某个（一个独立常体类型）B的实例，但是B实例服务的对象可能会随时变化。 (iff (GenericallyDependentContinuant a) (and (Continuant a) (exists (b t) (genericallyDependsOnAt a b t)))) // axiom label in BFO2 CLIF: [074-001] (iff (GenericallyDependentContinuant a) (and (Continuant a) (exists (b t) (genericallyDependsOnAt a b t)))) // axiom label in BFO2 CLIF: [074-001] generically dependent continuant 普遍依赖性常体 material material 物质 MaterialEntity MaterialEntity 物质实体(MaterialEntity) material entity 物质实体 a flame a forest fire a human being a hurricane a photon a puff of smoke a sea wave a tornado an aggregate of human beings. an energy wave an epidemic the undetached arm of a human being 一个人 一个人的无袖手臂 一个光子 一个海浪 一个能量波 一场流行病 一阵烟雾 一阵龙卷风 人类的一个总和。 森林大火 火焰 飓风 An independent continuant that is spatially extended whose identity is independent of that of other entities and can be maintained through time. 一个独立常体，是空间扩展的，其性质独立于其他实体，可以通过时间来维持。 BFO 2 Reference: Material entities (continuants) can preserve their identity even while gaining and losing material parts. Continuants are contrasted with occurrents, which unfold themselves in successive temporal parts or phases [60 BFO 2 Reference: Object, Fiat Object Part and Object Aggregate are not intended to be exhaustive of Material Entity. Users are invited to propose new subcategories of Material Entity. BFO 2 Reference: ‘Matter’ is intended to encompass both mass and energy (we will address the ontological treatment of portions of energy in a later version of BFO). A portion of matter is anything that includes elementary particles among its proper or improper parts: quarks and leptons, including electrons, as the smallest particles thus far discovered; baryons (including protons and neutrons) at a higher level of granularity; atoms and molecules at still higher levels, forming the cells, organs, organisms and other material entities studied by biologists, the portions of rock studied by geologists, the fossils studied by paleontologists, and so on.Material entities are three-dimensional entities (entities extended in three spatial dimensions), as contrasted with the processes in which they participate, which are four-dimensional entities (entities extended also along the dimension of time).According to the FMA, material entities may have immaterial entities as parts – including the entities identified below as sites; for example the interior (or ‘lumen’) of your small intestine is a part of your body. BFO 2.0 embodies a decision to follow the FMA here. BFO 2参考文献：“物质”旨在涵盖质量和能量（我们将在BFO的后续版本中介绍对部分能量的本体处理）。物质的一部分是任何包括基本粒子在内的适当或不适当的部分：夸克和轻子，包括电子，是迄今为止发现的最小的粒子；重子粒子在更高的粒度水平（包括质子和中子）；原子和分子的含量更高，形成由生物学家研究的细胞、器官、生物体和其他物质实体，由地质学家研究的岩石部分，由古生物学家研究的化石等等。物质实体是三维实体（在三个空间维度上扩展的实体），与它们参与的过程相反，它们是四维实体 （实体也沿着时间维度延伸）。根据FMA，物质实体可以有作为部分的非物质实体-包括下面标识为位置的实体；例如你的小肠的内部（或“腔”）是您身体的一部分。 BFO 2.0在这里体现了遵循FMA的决定。 BFO 2参考：对象，许可对象部分和对象聚合并是材料实体的细节。邀请用户提出物质实体的新子类别。 BFO 2参考：物质实体（常体）甚至可以在获得和丢失物质部分时保持其性质。常体与行体形成对比，后者在连续的时间部分或阶段展开自己[60] A material entity is an independent continuant that has some portion of matter as proper or improper continuant part. (axiom label in BFO2 Reference: [019-002]) 一个物质实体是一个独立常体，它的某些部分是适当的或不适当的常体部分。 （BFO2公理标签参考：[019-002]） Every entity which has a material entity as continuant part is a material entity. (axiom label in BFO2 Reference: [020-002]) every entity of which a material entity is continuant part is also a material entity. (axiom label in BFO2 Reference: [021-002]) 以一个物质实体作为常体部分的每个实体都是物质实体。 （BFO2公理标签参考：[020-002]） 物质实体为常体部分的每个实体也是物质实体。 （BFO2公理标签参考：[021-002]） (forall (x) (if (MaterialEntity x) (IndependentContinuant x))) // axiom label in BFO2 CLIF: [019-002] (forall (x) (if (MaterialEntity x) (IndependentContinuant x))) // axiom label in BFO2 CLIF: [019-002] (forall (x) (if (and (Entity x) (exists (y t) (and (MaterialEntity y) (continuantPartOfAt x y t)))) (MaterialEntity x))) // axiom label in BFO2 CLIF: [021-002] (forall (x) (if (and (Entity x) (exists (y t) (and (MaterialEntity y) (continuantPartOfAt x y t)))) (MaterialEntity x))) // axiom label in BFO2 CLIF: [021-002] (forall (x) (if (and (Entity x) (exists (y t) (and (MaterialEntity y) (continuantPartOfAt y x t)))) (MaterialEntity x))) // axiom label in BFO2 CLIF: [020-002] (forall (x) (if (and (Entity x) (exists (y t) (and (MaterialEntity y) (continuantPartOfAt y x t)))) (MaterialEntity x))) // axiom label in BFO2 CLIF: [020-002] bfo BFO:0000040 material entity 物质实体 immaterial ImmaterialEntity BFO 2 Reference: Immaterial entities are divided into two subgroups:boundaries and sites, which bound, or are demarcated in relation, to material entities, and which can thus change location, shape and size and as their material hosts move or change shape or size (for example: your nasal passage; the hold of a ship; the boundary of Wales (which moves with the rotation of the Earth) [38, 7, 10 immaterial entity 非物质实体 anatomical structure 解剖结构 material anatomical entity 物质解剖实体 cell part 细胞部分 Catechol in which the hydrogen at position 4 is substituted by a 2-aminoethyl group. 0 C8H11NO2 InChI=1S/C8H11NO2/c9-4-3-6-1-2-7(10)8(11)5-6/h1-2,5,10-11H,3-4,9H2 VYFYYTLLBUKUHU-UHFFFAOYSA-N 153.17840 153.079 NCCc1ccc(O)c(O)c1 CHEBI:11695 CHEBI:11930 CHEBI:14203 CHEBI:1764 CHEBI:23886 CHEBI:43686 CAS:51-61-6 DrugBank:DB00988 Drug_Central:947 HMDB:HMDB0000073 KEGG:C03758 KEGG:D07870 KNApSAcK:C00001408 LINCS:LSM-4630 MetaCyc:DOPAMINE PMID:10629745 PMID:11149432 PMID:9422813 Reaxys:1072822 Wikipedia:Dopamine 4-(2-aminoethyl)benzene-1,2-diol Dopamine chebi_ontology 2-(3,4-Dihydroxyphenyl)ethylamine 2-(3,4-dihydroxyphenyl)ethylamine 3,4-Dihydroxyphenethylamine 3-Hydroxytyramine 4-(2-Aminoethyl)-1,2-benzenediol 4-(2-Aminoethyl)benzene-1,2-diol 4-(2-aminoethyl)-1,2-benzenediol 4-(2-aminoethyl)catechol 4-(2-aminoethyl)pyrocatechol Deoxyepinephrine Hydroxytyramin dopamina dopamine dopaminum CHEBI:18243 dopamine Any constitutionally or isotopically distinct atom, molecule, ion, ion pair, radical, radical ion, complex, conformer etc., identifiable as a separately distinguishable entity. chebi_ontology CHEBI:23367 molecular entity A 3-hydroxy steroid that is estra-1,3,5(10)-triene substituted by hydroxy groups at positions 3 and 17. 0 C18H24O2 InChI=1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17?,18+/m1/s1 VOXZDWNPVJITMN-WKUFJEKOSA-N 272.38196 272.178 [H][C@]12CC[C@]3(C)C(O)CC[C@@]3([H])[C@]1([H])CCc1cc(O)ccc21 CHEBI:42364 PMID:10696569 PMID:24084694 Wikipedia:Estradiol estra-1,3,5(10)-triene-3,17-diol chebi_ontology oestradiol CHEBI:23965 estradiol A chemical entity is a physical entity of interest in chemistry including molecular entities, parts thereof, and chemical substances. 化学实体是对化学感兴趣的物理实体，包括分子实体、其部分及化学物质。 chemical entity 化学实体 chebi_ontology CHEBI:24431 chemical entity 化学实体 A 3-hydroxy steroid that is estra-1,3,5(10)-trien-3-ol substituted by additional hydroxy groups at positions 16 and 17 (16alpha,17beta-stereoisomer). 0 C18H24O3 InChI=1S/C18H24O3/c1-18-7-6-13-12-5-3-11(19)8-10(12)2-4-14(13)15(18)9-16(20)17(18)21/h3,5,8,13-17,19-21H,2,4,6-7,9H2,1H3/t13-,14-,15+,16-,17+,18+/m1/s1 PROQIPRRNZUXQM-ZXXIGWHRSA-N 288.38140 288.173 [H][C@]12CC[C@]3(C)[C@@H](O)[C@H](O)C[C@@]3([H])[C@]1([H])CCc1cc(O)ccc21 CHEBI:23969 CHEBI:42467 CHEBI:4869 Beilstein:2508172 CAS:50-27-1 DrugBank:DB04573 Drug_Central:3187 HMDB:HMDB0000153 KEGG:C05141 KEGG:D00185 LINCS:LSM-2368 LIPID_MAPS_instance:LMST02010003 MetaCyc:ESTRIOL PDBeChem:ESL PMID:11133265 PMID:23777262 PMID:9373313 Reaxys:2508172 Wikipedia:Estriol ESTRIOL Estriol estra-1,3,5(10)-triene-3,16alpha,17beta-triol chebi_ontology (16alpha,17beta)-estra-1,3,5(10)-triene-3,16,17-triol 1,3,5(10)-Estratriene-3,16-alpha,17beta-triol 16alpha,17beta-estriol 16alpha-hydroxyestradiol 3,16alpha,17beta-trihydroxy-Delta(1,3,5)-estratriene Estriel Oestriol oestriol trihydroxyestrin CHEBI:27974 estriol A primary amino compound that is the 5-hydroxy derivative of tryptamine. 0 C10H12N2O InChI=1S/C10H12N2O/c11-4-3-7-6-12-10-2-1-8(13)5-9(7)10/h1-2,5-6,12-13H,3-4,11H2 QZAYGJVTTNCVMB-UHFFFAOYSA-N 176.215 176.095 C1=CC(=CC=2C(=CNC12)CCN)O CHEBI:1420 CHEBI:26652 CHEBI:49894 Beilstein:143524 CAS:50-67-9 Gmelin:1861995 HMDB:HMDB0000259 KEGG:C00780 KNApSAcK:C00001429 LINCS:LSM-6589 MetaCyc:SEROTONIN PDBeChem:SRO PMID:18593914 PMID:22770225 PMID:24136337 Reaxys:143524 Wikipedia:Serotonin 3-(2-aminoethyl)-1H-indol-5-ol SEROTONIN Serotonin chebi_ontology 3-(2-Aminoethyl)-1H-indol-5-ol 5-HT 5-Hydroxytryptamine Enteramine serotonine thrombocytin thrombotonin CHEBI:28790 serotonin Any substituent group or skeleton containing carbon. chebi_ontology CHEBI:33247 organic group 4-(2-Aminoethyl)pyrocatechol [4-(2-aminoethyl)benzene-1,2-diol] and derivatives formed by substitution. 0 C8H9NO2R2 151.163 151.063 CHEBI:23056 CHEBI:3468 KEGG:C02012 Catecholamine catecholamines chebi_ontology catecholamines CHEBI:33567 catecholamine A catecholamine in which the aminoethyl side-chain is hydroxy-substituted at C-1 and methylated on nitrogen. 0 C9H13NO3 InChI=1S/C9H13NO3/c1-10-5-9(13)6-2-3-7(11)8(12)4-6/h2-4,9-13H,5H2,1H3 UCTWMZQNUQWSLP-UHFFFAOYSA-N 183.20446 183.090 CNCC(O)c1ccc(O)c(O)c1 Beilstein:2212160 CAS:329-65-7 Drug_Central:4508 Gmelin:51559 LINCS:LSM-4958 PMID:10052027 PMID:24252294 PMID:24719616 Reaxys:2212160 4-[1-hydroxy-2-(methylamino)ethyl]benzene-1,2-diol chebi_ontology (+-)-adrenaline (+-)-epinephrine 2-(methylamino)-1-(3,4-dihydroxyphenyl)ethanol dl-adrenaline epinephrine racemic racepinefrina racepinefrine racepinefrinum CHEBI:33568 adrenaline When two or more amino acids combine to form a peptide, the elements of water are removed, and what remains of each amino acid is called an amino-acid residue. amino acid residue protein residue chebi_ontology CHEBI:33708 amino-acid residue C4H6N2O3R2(C7H9N2O2R)n CAS:9064-67-9 KEGG:C00211 Collagen 胶原蛋白 chebi_ontology CHEBI:3815 Collagen 胶原蛋白 A compound formally derived from ammonia by replacing one, two or three hydrogen atoms by organyl groups. chebi_ontology CHEBI:50047 organic amino compound A bicyclic macrolide natural product consisting of a 16-membered bicyclic lactone attached to the rare 2-thiazolidinone moiety. It is obtained from the Red Sea sponge Latrunculia magnifica and from the Fiji Islands sponge Cacospongia mycofijiensis. Latrunculin A inhibits actin polymerisation, microfilament organsation and microfilament-mediated processes. 一种双环大环内酯类天然产物，由一种十六元的双环内酯，附在罕见的2-噻唑酮中形成。它是取自红海海绵Latrunculia magnifica和斐济群岛海绵Cacospongia mycofijiensis。微丝解聚剂抑制肌动蛋白聚合，微丝组织和微丝介导的过程。 0 C22H31NO5S InChI=1S/C22H31NO5S/c1-15-7-5-3-4-6-8-16(2)11-20(24)27-18-12-17(10-9-15)28-22(26,13-18)19-14-29-21(25)23-19/h3-5,7,11,15,17-19,26H,6,8-10,12-14H2,1-2H3,(H,23,25)/b4-3+,7-5-,16-11-/t15-,17-,18-,19+,22-/m1/s1 DDVBPZROPPMBLW-IZGXTMSKSA-N 421.55000 421.192 [H][C@]1(CSC(=O)N1)[C@@]1(O)C[C@H]2C[C@@H](CC[C@H](C)\C=C/C=C/CC\C(C)=C/C(=O)O2)O1 CHEBI:43626 CAS:76343-93-6 PDBeChem:LAR PMID:10859320 PMID:12087066 PMID:17897856 PMID:19528469 PMID:20677927 PMID:22492972 PMID:22498812 PMID:3200109 PMID:3556584 PMID:9250716 Reaxys:4239363 (4R)-4-[(1R,4Z,8E,10Z,12S,15R,17R)-17-hydroxy-5,12-dimethyl-3-oxo-2,16-dioxabicyclo[13.3.1]nonadeca-4,8,10-trien-17-yl]-1,3-thiazolidin-2-one LATRUNCULIN A 拉春库林A chebi_ontology (+)-latrunculin A (+)-拉春库林A (4R)-4-[(1R,4Z,8E,10Z,12S,15R,17R)-17-hydroxy-5,12-dimethyl-3-oxo-2,16-dioxabicyclo[13.3.1]nonadeca-4,8,10-trien-17-yl]-1,3-thiazolidin-2-one LAT-A LatA NSC 613011 CHEBI:69136 latrunculin A 拉春库林A a maintaining cell culture process that keeps cell line cell alive and actively cultured. 维持细胞株存活和活跃培养的细胞培养过程。 YH YH cell line cell culturing 细胞系细胞培养 cell line cell 细胞系细胞 A cultured cell that is part of a cell line - a stable and homogeneous population of cells with a common biological origin and propagation history in culture A cultured cell that is part of a cell line - a stable and homogeneous population of cells with a common biological origin and propagation history in culture 一个培养细胞，它是一个细胞株（一个稳定的，同质的细胞群，具有共同的生物学起源和增殖史）的一部分 一个培养细胞，它是一个细胞株（一个稳定的，同质的细胞群，具有共同的生物学起源和增殖史）的一部分 Yongqun He, Matthew Brush, Sirarat Sarntivijai, Alexander Diehl, Jie Zheng, Yu Lin, Bjoern Peters Yongqun He, Matthew Brush, Sirarat Sarntivijai, Alexander Diehl, Jie Zheng, Yu Lin, Bjoern Peters A 'cell line cell' is a part of a cell line established through the passaging/selection of a primary cultured cells or the experimental modification of an existing cell line. New types of cell line cells are established after sufficient passaging of a primary culture to establish a stable and homogenous population that qualifies as a line (typically 1-20 passages), or following some spontaneous or experimental modification that confers novel characteristics to an existing line. A cell line cell typically has mutations of five or more genes compared to the original cell that derives the cell line cell. Some gene mutations may turn on some oncogenes. Cell line cells can be in active culture, stored in a quiescent state for future use (e.g. frozen in liquid nitrogen), or applied in experimental procedures. “细胞系细胞”是通过原代培养细胞的传代/选择或现有细胞系的实验性修饰而建立的细胞系的一部分。新类型的细胞系细胞的建立过程是原代培养物充分传代后以建立符合条件（典型地1-20代）的稳定且同质的群体，或者在某些自发或实验性修饰之后赋予现有细胞系新的特征。与产生细胞系细胞的原始细胞相比，细胞系细胞通常具有5个或更多个基因的突变。 一些基因突变可能会开启一些致癌基因。 细胞系细胞可处于活性培养物中，以静止状态储存以供将来使用（例如在液氮中冷冻），或者在实验程序使用。 cell line cell 细胞系细胞 suspension cell line culturing is a cell line culturing method where cells do not require a surface for growth and differentiation. This method is appropriate for cells that are adapted to suspension culture or cells that are non-adhesive (e.g. hematopoietic cells) 悬浮细胞系培养是其中细胞不需要生长和分化表面的细胞系培养方法。 该方法适用于适合悬浮培养的细胞或非贴壁细胞（如造血细胞） suspension cell line culturing 悬浮细胞培养 adherent cell line culturing is a cell line culturing method where cells require a surface for growth and differentiation. 贴壁细胞系培养是一种细胞系培养方法，其中细胞需要一个表面进行生长和分化。 adherent cell line culturing 贴壁细胞系培养 a role that inheres in a cell line repository organization. 一个细胞系资源组织中所起的作用 SS, YH SS, YH cell line repository role 细胞库角色 an organization that serves as a repository of cell lines. 一个作为细胞系存储库的组织。 SS, YH SS, YH cell line repository 细胞库 an immortal human lymphocyte cell line cell that is derived from T cell. 一种源自T细胞的永生人淋巴细胞系细胞。 YH YH immortal human T cell line cell 永生的人T细胞系细胞 adherent on feeder cells cell line culturing 在饲养细胞上贴壁培养细胞系 adherent-patchy cell line culturing 贴壁斑片状细胞培养 loosely adherent cell line culturing 松散贴壁细胞培养 A cell line cell that is capable of replicating a limited number of times in culture before undergoing senescence. Yongqun He, Matthew Brush, Sirarat Sarntivijai finite cell line cell 有限细胞系细胞 mortal cell line cell 非永生细胞系细胞 immortal cell line cell 永生细胞系细胞 A cell line cell that is expected to be capable of an unlimited number of divisions, and is thus able to support indefinite propagation in vitro as part of an immortal cell line. 预期能够进行无限次分裂的细胞系细胞，作为永生细胞系一部分，能够支持体外无限增殖。 Yongqun He, Matthew Brush, Allen Xiang, Asiyah Yu Lin, Sirarat Sarntivijai, James Malone, Jie Zheng, Tomasz Adamusiak Yongqun He, Matthew Brush, Allen Xiang, Asiyah Yu Lin, Sirarat Sarntivijai, James Malone, Jie Zheng, Tomasz Adamusiak continuous cell line cell permanent cell line cell 永久细胞系细胞 连续细胞系细胞 immortal cell line cell 永生细胞系细胞 YH YH a immortal cell line that is dervied from human tissue. 一种源自人体组织的永生细胞系。 immortal human cell line 永生的人细胞系 immortal human cell line cell 永生的人细胞系细胞 An immortal skin-derived cell line cell that derives from frog. 源自青蛙的永生皮肤衍生的细胞系细胞。 Yongqun He immortal frog cell line cell 永生的蛙细胞系细胞 a cell culture growth mode that indicates a cell needs to adhere to a cell culture plate to grow. 一种细胞培养生长模式：细胞需要粘附在细胞培养板上生长 YH YH adherent cell culture growth mode 贴壁细胞培养生长模式 a cell culture growth mode that indicates a cell can grow when the cell is suspended in cell culture medium. 一种细胞培养生长模式：当细胞悬浮在细胞培养基中时，细胞可以生长 YH YH suspension cell culture growth mode 悬浮细胞培养生长模式 a disposition that represents a mode of cell culture growth, i.e., the cell will grow at a specific cell culture mode. For example, an adherent cell culture growh mode means that the cell will grow when the cell is adhere to a cell culture plate. 一种代表细胞培养生长模式的配置，即细胞将以特定细胞培养模式生长。 例如，贴壁细胞培养生长模式意味着当细胞粘附于细胞培养板时细胞将生长。 YH YH cell culture growth mode 细胞培养生长模式 cell line 细胞系 He, Tong-Chuan, et al., Identification of c-MYC as a target of the APC pathway. Science 281.5382 (1998): 1509-1512.: "To evaluate the transcriptional effects of APC, we studied a human colorectal cancer cell line (HT29-APC) containing a zinc-inducible APC gene and a control cell line (HT29–β-Gal) containing an analogous inducible lacZ gene". Note that common usage in the literature is often of the form "a human colorectal cancer cell line", as seen above. But such references to studies in "a line" refer to the fact that discrete populations of cells that are input into culturing or experiments, not an entire lineage of cells. It is these discrete populations that we refer to as 'cell lines'. He, Tong-Chuan, et al., Identification of c-MYC as a target of the APC pathway. Science 281.5382 (1998): 1509-1512.: "为了评估APC的转录效应，我们研究了含有锌诱导型APC基因的人结直肠癌细胞系（HT29-APC）和含有类似诱导型lacZ基因的对照细胞系（HT29-β-Gal）". 请注意，文献中的常见用法通常是“人类结直肠癌细胞系”的形式",如上所见。 但是对"一个细胞系"研究的这种引用，指的是输入培养或实验的离散细胞群体，而不是整个细胞系。 我们将这些离散的群体称为'细胞系'。 A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). 一个培养细胞群体，其代表具有共同增殖历史（即已经在培养中一起连续传代）的遗传稳定且同质的培养细胞群体。 培养的细胞群体代表具有共同繁殖历史（即已经在培养中一起连续传代）的遗传稳定且同质的培养细胞群体。 In the spring of 2013, a working group comprised of domain experts and representatives from CLO, OBI, CL, and ReO worked to establish a consensus model and definitions of cultured cells across these efforts. This included a careful characterization of how the term 'cell line' should be defined and applied. Notes about this work and its outcomes can be found on the CLO wiki here: http://code.google.com/p/clo-ontology/wiki/Cell_Lines 在2013年春季，由CLO，OBI，CL和ReO的领域专家和代表组成的工作组经过这些努力中建立了共识模型和培养细胞的定义。 这包括仔细描述术语“细胞系”应该如何定义和应用。 关于这项工作及其结果的说明可以在CLO wiki上找到： http://code.google.com/p/clo-ontology/wiki/Cell_Lines MB, SS, JZ, MAH, BP, CS, YH MB, SS, JZ, MAH, BP, CS, YH The term 'line' is used when a culture has undergone an intentional experimental process to establish a more uniform and stable population of cells (see 'establishing cell line'). This will require one or more passages, but may involve additional selection processes. Through such passaging and/or selection processes, the resulting 'line' attains some level of genetic stability and compositional homogeneity which is typically absent in primary cultures. Because of their relative homogeneity, ‘lines’ are capable of being characterized and stably propagated over a period of time. A new *type* of cell line can be established not only through the passaging/selection of a primary culture, but also through experimental modifications of existing lines (e.g. immortalization, stable genetic modifications, drug selection for a resistant subset, etc.). The definition provided here establishes the 'scale' of cell populations that qualify as cell lines - specifically those with a shared propagation history in culture. In this way, the 'cell line' class demarcates populations that represent what researchers actually use in the practice of science - e.g. as inputs to culturing, experimentation, and sharing. The definition is such that cell lines will exhibit important attributes. For example, they will have a relatively homogenous cell type composition as they have experienced similar selective pressures due to their continuous co-propagation. In addition, these populations can also be characterized by a passage number, again owing to their common passaging history.  As defined here, 'cell line' can refer to a population of cells in active culture, applied experimentally, or stored in a quiescent state for future use. 当培养物经过有意的实验过程以建立更均匀和稳定的细胞群时（参见“建立细胞系”），使用术语“系”。这将需要一个或多次传代，但可能涉及额外的选择过程。通过这样的传代和/或选择过程，产生的'细胞系'达到一定水平的遗传稳定性和组成均一性，这在原代培养物中通常是不存在的。由于它们的相对同质性，“细胞系”能够在一段时间内被表征和稳定地增殖。一种新*型*细胞系不仅可以通过原代培养物的传代/选择建立，而且可以通过对现有系列的实验性修饰（例如永生化，稳定的遗传修饰，用于抗性子集的药物选择等）来建立。 这里提供的定义确立了符合细胞系特征的细胞群的“范围” - 特别是那些在培养中具有共同繁殖历史的细胞群。通过这种方式，“细胞系”类划分代表研究人员在科学实践中实际使用的细胞群 - 例如作为培养、实验和分享的投入。定义是这样的，细胞系将显示重要的属性。例如，它们将具有相对均匀的细胞类型组成，因为它们的连续共增殖而经历了类似的选择压力。此外，这些细胞群还可以通过传代数来表征，这也是由于它们共同的传代史。如此处所定义的，“细胞系”可以指活性培养物中，实验性应用的或以静止状态存储以备将来使用的细胞群。 cell line 细胞系 mixed adherent and suspension cell line culturing 混合贴壁悬浮细胞培养 clusters in suspension cell line culturing 在悬浮细胞系培养中的成簇细胞 a immortal cell line that is dervied from mouse tissue. 从老鼠组织中得到的一种永生细胞系。 Yue Liu, Yongqun He Yue Liu, Yongqun He immortal mouse cell line 永生的小鼠细胞系 immortal mouse cell line cell 永生的小鼠细胞系细胞 a cell line that is derived from non-terminally differentiated cell 源自非终末分化细胞的细胞系 ZX, YH immortal non-terminally differentiated cell line 永生的非终末分化细胞系 immortal non-terminally differentiated cell line cell 永生的非终末分化细胞系细胞 a immortal non-terminally differentiated cell line that is derived from muscle precursor cell 源自肌前体细胞的永生非终末分化细胞系 ZX, YH immortal muscle precursor cell line 永生的肌前体细胞系 immortal muscle precursor cell line cell 永生的肌前体细胞系细胞 a immortal muscle precursor cell line that is derived from myoblast 源自成肌细胞的永生肌肉前体细胞系 ZX, YH immortal myoblast cell line 永生的成肌细胞系 immortal myoblast cell line cell 永生的成肌细胞系细胞 a cell line that is derived from secretory cell 源自分泌细胞的细胞系 ZX, YH immortal secretory cell line 永生的分泌细胞系 immortal secretory cell line cell 永生的分泌细胞系细胞 a immortal secretory cell line that is derived from endocrine cell 源自内分泌细胞的永生分泌细胞系 ZX, YH immortal endocrine cell line 永生的内分泌细胞系 immortal endocrine cell line cell 永生的内分泌细胞系细胞 a immortal endocrine cell line that is derived from mast cell 由肥大细胞衍生的永生内分泌细胞系 ZX, YH immortal mast cell line 永生的肥大细胞系 immortal mast cell line cell 永生的肥大细胞系细胞 a immortal secretory cell line that is derived from plasma cell 源自浆细胞的永生分泌细胞系 ZX, YH immortal plasma cell line 永生的浆细胞系 immortal plasma cell line cell 永生的浆细胞系细胞 an immortal cell line cell that is derived from contractile cell 源自收缩细胞的永生细胞系细胞 ZX, YH immortal contractile cell line cell 永生化的收缩细胞系细胞 a immortal contractile cell line cell that is derived from muscle cell 源自肌肉细胞的永生收缩细胞系细胞 ZX, YH immortal muscle cell line cell 永生的肌肉细胞系细胞 an immortal cell line cell that is derived from electrically active cell 源自电活性细胞的永生细胞系细胞 ZX, YH immortal electrically active cell line cell 永生的电活性细胞系细胞 a immortal electrically active cell line cell that is derived from electrically responsive cell 源自电反应细胞的永生电活性细胞系细胞 ZX, YH immortal electrically responsive cell line cell 永生的电反应细胞系细胞 a immortal electrically responsive cell line cell that is derived from neuron 源自神经元的永生电反应细胞系细胞 ZX, YH immortal neuron cell line cell 永生的神经元细胞系细胞 a immortal neuron cell line cell that is derived from bipolar neuron 源自双极神经元的永生神经元细胞系细胞 ZX, YH immortal bipolar neuron cell line cell 永生的双极神经元细胞系细胞 a immortal neuron cell line cell that is derived from peripheral neuron 源自外周神经元的永生神经元细胞系细胞 ZX, YH immortal peripheral neuron cell line cell 永生的外周神经元细胞系细胞 a cell line that is derived from motile cell 源自运动细胞的细胞系 ZX, YH immortal motile cell line 永生的游动细胞系 immortal motile cell line cell 永生的游动细胞系细胞 a immortal motile cell line that is derived from phagocyte 源自吞噬细胞的永生游动细胞系， ZX, YH immortal phagocyte cell line cell 永生的吞噬细胞细胞系细胞 a immortal phagocyte cell line that is derived from macrophage 源自巨噬细胞的永生吞噬细胞系 ZX, YH immortal macrophage cell line cell 永生的巨噬细胞系细胞 a immortal motile cell line that is derived from leukocyte 源自白细胞的永生游动细胞系 ZX, YH immortal leukocyte cell line 永生的白细胞系 immortal leukocyte cell line cell 永生的白细胞系细胞 a immortal leukocyte cell line that is derived from myeloid leukocyte 源自髓系白细胞的永生白细胞系 ZX, YH immortal myeloid leukocyte cell line 永生的髓样白血球细胞系 immortal myeloid leukocyte cell line cell 永生的髓样白血球细胞系细胞 a immortal myeloid leukocyte cell line that is derived from monocyte 源自单核细胞的永生髓系白细胞系 ZX, YH immortal monocyte cell line 永生的单核细胞系 immortal monocyte cell line cell 永生的单核细胞系细胞 a immortal leukocyte cell line that is derived from nongranular leukocyte 源自无粒白细胞的永生白细胞细胞系 ZX, YH immortal nongranular leukocyte cell line 永生的无颗粒白细胞细胞系 immortal nongranular leukocyte cell line cell 永生的无颗粒白细胞细胞系细胞 a immortal nongranular leukocyte cell line that is derived from lymphocyte 源自淋巴细胞的永生无颗粒白细胞细胞系 ZX, YH immortal lymphocyte cell line 永生的淋巴细胞系 immortal lymphocyte cell line cell 永生的淋巴细胞系细胞 a immortal lymphocyte cell line that is derived from T cell 源自T细胞的永生淋巴细胞系 ZX, YH immortal T cell line 永生的T细胞系 immortal T cell line cell 永生的T细胞系细胞 a immortal lymphocyte cell line that is derived from B cell 源自B细胞的永生淋巴细胞系 ZX, YH immortal B cell line 永生的B细胞系 immortal B-cell line cell 永生的B-细胞系细胞 immortal B cell line cell 永生的B细胞系细胞 a immortal cell line cell that is derived from eukaryotic cell 源自真核细胞的永生细胞系细胞 ZX, YH immortal eukaryotic cell line cell 永生的真核细胞系细胞 a immortal eukaryotic cell line that is derived from animal cell 源自动物细胞的永生真核细胞系 ZX, YH immortal animal cell line 永生的动物细胞系 immortal animal cell line cell 永生的动物细胞系细胞 a immortal animal cell line that is derived from epithelial cell 源自上皮细胞的永生动物细胞系 ZX, YH immortal epithelial cell line cell 永生的上皮细胞系细胞 a immortal animal cell line that is derived from hematopoietic cell 源自造血细胞的永生动物细胞系 ZX, YH immortal hematopoietic cell line 永生的造血细胞系 immortal hematopoietic cell line cell 永生的造血细胞系细胞 a immortal hematopoietic cell line that is derived from megakaryocyte-erythroid progenitor cell 源自巨核细胞-红细胞祖细胞的永生造血细胞系 ZX, YH immortal megakaryocyte-erythroid progenitor cell line 永生的巨细胞红细胞祖细胞系 immortal megakaryocyte-erythroid progenitor cell line cell 永生的巨细胞红细胞祖细胞系细胞 a immortal hematopoietic cell line that is derived from blood cell 源自血细胞的永生造血细胞系 ZX, YH immortal blood cell line 永生的血细胞系 immortal blood cell line cell 永生的血细胞系细胞 a immortal hematopoietic cell line that is derived from myeloid cell 源自骨髓细胞的永生造血细胞系 ZX, YH immortal myeloid cell line 永生的骨髓细胞系 immortal myeloid cell line cell 永生的骨髓细胞系细胞 a immortal hematopoietic cell line that is derived from hematopoietic lineage restricted progenitor cell 源自造血谱系限制性祖细胞的永生造血细胞系 ZX, YH immortal hematopoietic lineage restricted progenitor cell line 永生的造血系限制性祖细胞系 immortal hematopoietic lineage restricted progenitor cell line cell 永生的造血系限制性祖细胞系细胞 a immortal hematopoietic lineage restricted progenitor cell line that is derived from myeloblast 源自成髓细胞的永生造血谱系限制性祖细胞系 ZX, YH immortal myeloblast cell line 永生的成髓细胞系 immortal myeloblast cell line cell 永生的成髓细胞系细胞 a immortal animal cell line that is derived from neural cell 源自神经细胞的永生动物细胞系 ZX, YH immortal neural cell line 永生的神经细胞系 immortal neural cell line cell 永生的神经细胞系细胞 a immortal animal cell line that is derived from connective tissue cell 源自结缔组织细胞的永生动物细胞系 ZX, YH immortal connective tissue cell line cell 永生的结缔组织细胞系细胞 a immortal connective tissue cell line that is derived from fibroblast 源自成纤维细胞的永生结缔组织细胞系 ZX, YH immortal fibroblast cell line 永生的成纤维细胞系 immortal fibroblast cell line cell 永生的成纤维细胞系细胞 a cell line that is derived from stuff accumulating cell 源自积聚细胞的细胞系。 ZX, YH immortal stuff accumulating cell line cell 永生的积聚细胞系细胞 a cell line that is derived from nucleate cell 源自有核细胞的细胞系 ZX, YH immortal nucleate cell line 永生的核细胞系 immortal nucleate cell line cell 永生的核细胞系细胞 a cell line that is derived from somatic cell 源自体细胞的细胞系 ZX, YH immortal somatic cell line cell 永生的体细胞系细胞 a immortal epithelial cell line cell that is derived from HeLa cell 源自HeLa细胞的永生上皮细胞系细胞 Yongqun He Yongqun He HeLa derived cell line cell HeLa衍生细胞系细胞 an immortal cell line cell that is established by fusing a specific antibody-producing B cell with a myeloma (B cell cancer) cell that is selected for its ability to produce a monoclonal antibody. 通过将产生特异性抗体的B细胞与针对其产生单克隆抗体的能力而选择的骨髓瘤（B细胞癌）进行细胞融合而建立的永生细胞系细胞。 The production of monoclonal antibodies using the hybridoma technology was invented by Cesar Milstein and Georges J. F. Köhler in 1975. They shared the Nobel Prize of 1984 for Medicine and Physiology with Niels Kaj Jerne, who made other contributions to immunology. 利用杂交瘤技术生产单克隆抗体是由Cesar Milstein和Georges J. F.Köhler于1975年发明的。他们与为免疫学做出贡献的Niels Kaj Jerne共同获得了1984年的诺贝尔医学和生理学奖。 Yongqun He Yongqun He WEB: http://en.wikipedia.org/wiki/Hybridoma_technology WEB: http://en.wikipedia.org/wiki/Hybridoma_technology hybridoma cell line cell 杂交瘤细胞系细胞 An immortal B cell line cell that derives from peripheral vein. 源自外周静脉的永生B细胞系细胞 Yue Liu, Yongqun He immortal peripheral vein-derived B cell line cell 永生的外周静脉衍生B细胞系细胞 An immortal cell line cell that derives from organism part. 源自生物体部分的永生细胞系细胞。 Yue Liu, Yongqun He immortal organism part-derived cell line cell 永生的生物部分衍生细胞系细胞 An immortal T cell line cell that derives from peripheral vein. 源自外周静脉的永生T细胞系细胞。 Yue Liu, Yongqun He immortal peripheral vein-derived T cell line cell 永生的外周静脉衍生T细胞系细胞 An immortal fibroblast cell line cell that derives from animal ovary. 源自动物卵巢的永生成纤维细胞系细胞。 Yue Liu, Yongqun He immortal animal ovary-derived fibroblast cell line cell 永生的动物卵巢衍生成纤维细胞系细胞 An immortal fibroblast cell line cell that derives from lung. 源自肺的永生成纤维细胞系细胞。 Yue Liu, Yongqun He immortal lung-derived fibroblast cell line cell 永生的肺衍生成纤维细胞系细胞 An immortal cell line cell that derives from skin. 源自皮肤的永生细胞系细胞。 Yue Liu, Yongqun He immortal skin-derived cell line cell 永生的皮肤衍生细胞系细胞 An immortal cell line cell that derives from vasculature. 源自血管系统的永生细胞系细胞。 Yue Liu, Yongqun He immortal vasculature-derived cell line cell 永生化的血管衍生细胞系细胞 An immortal cell line cell that derives from fetal membrane. 源自胎膜的永生细胞系细胞。 Yue Liu, Yongqun He immortal fetal membrane-derived cell line cell 永生的胎膜衍生细胞系细胞 An immortal cell line cell that derives from dento-alveolar joint. 源自牙齿-牙槽骨关节的永生细胞系细胞。 Yue Liu, Yongqun He immortal dento-alveolar joint-derived cell line cell 永生的牙齿-牙槽骨关节衍生细胞系细胞 An immortal cell line cell that derives from mixed endoderm/mesoderm-derived structure. 源自混合内胚层/中胚层衍生结构的永生细胞系细胞。 Yue Liu, Yongqun He immortal mixed endoderm/mesoderm-derived structure-derived cell line cell 永生的混合内胚层/中胚层衍生结构衍生的细胞系细胞 An immortal cell line cell that derives from thymus. 源自胸腺的永生细胞系细胞。 Yue Liu, Yongqun He immortal thymus-derived cell line cell 永生的胸腺衍生细胞系细胞 An immortal cell line cell that derives from tongue. 源自舌头的永生细胞系细胞。 Yue Liu, Yongqun He immortal tongue-derived cell line cell 永生的舌衍生细胞系细胞 An immortal cell line cell that derives from pair of lungs. 源自一对肺的永生细胞系细胞。 Yue Liu, Yongqun He immortal lung-derived cell line cell 永生化的肺衍生细胞系细胞 An immortal cell line cell that derives from kidney. 源自肾的永生细胞系细胞。 Yue Liu, Yongqun He immortal kidney-derived cell line cell 永生化的肾衍生细胞系细胞 An immortal cell line cell that derives from placenta. 源自胎盘的永生细胞系细胞。 Yue Liu, Yongqun He immortal placenta-derived cell line cell 永生化的胎盘衍生细胞系细胞 An immortal cell line cell that derives from embryo. 源自胚胎的永生细胞系细胞。 Yue Liu, Yongqun He immortal embryo-derived cell line cell 永生化的胚胎衍生细胞系细胞 An immortal cell line cell that derives from prostate gland. 源自前列腺的永生细胞系细胞。 Yue Liu, Yongqun He immortal prostate gland-derived cell line cell 永生化的前列腺衍生细胞系细胞 An immortal cell line cell that derives from septum of telencephalon. 源自端脑中隔的永生细胞系细胞。 Yue Liu, Yongqun He immortal septum of telencephalon-derived cell line cell 永生的端脑中隔衍生细胞系细胞 An immortal cell line cell that derives from larva. 源自幼虫的永生细胞系细胞。 Yue Liu, Yongqun He immortal larva-derived cell line cell 永生的幼虫衍生细胞系细胞 An immortal cell line cell that derives from liver. 源自肝脏的永生细胞系细胞。 Yue Liu, Yongqun He immortal liver-derived cell line cell 永生的肝衍生细胞系细胞 An immortal cell line cell that derives from colon. 源自结肠的永生细胞系细胞。 Yue Liu, Yongqun He immortal colon-derived cell line cell 永生的结肠衍生细胞系细胞 An immortal cell line cell that derives from caecum. 源自盲肠的永生细胞系细胞。 Yue Liu, Yongqun He immortal caecum-derived cell line cell 永生的盲肠衍生细胞系细胞 An immortal cell line cell that derives from pancreas. 源自胰腺的永生细胞系细胞。 Yue Liu, Yongqun He immortal pancreas-derived cell line cell 永生的胰腺衍生细胞系细胞 An immortal cell line cell that derives from brain. 源自大脑的永生细胞系细胞。 Yue Liu, Yongqun He immortal brain-derived cell line cell 永生化的大脑衍生细胞系细胞 An immortal cell line cell that derives from collecting duct of renal tubule. 源自肾小管集合管的永生细胞系细胞。 Yue Liu, Yongqun He immortal collecting duct of renal tubule-derived cell line cell 永生的肾小管集合管衍生细胞系细胞 An immortal cell line cell that derives from female gonad. 源自雌性性腺的永生细胞系细胞。 Yue Liu, Yongqun He immortal female gonad-derived cell line cell 永生的雌性性腺衍生细胞系细胞 An immortal cell line cell that derives from testis. 源自睾丸的永生细胞系细胞。 Yue Liu, Yongqun He immortal testis-derived cell line cell 永生的睾丸衍生细胞系细胞 An immortal cell line cell that derives from stomach. 源自胃的永生细胞系细胞。 Yue Liu, Yongqun He immortal stomach-derived cell line cell 永生化的胃衍生细胞系细胞 An immortal cell line cell that derives from bone element. 源自骨元素的永生细胞系细胞。 Yue Liu, Yongqun He immortal bone element-derived cell line cell 永生的骨元素衍生细胞系细胞 An immortal cell line cell that derives from thyroid gland. 源自甲状腺的永生细胞系细胞。 Yue Liu, Yongqun He immortal thyroid gland-derived cell line cell 永生的甲状腺衍生细胞系细胞 An immortal cell line cell that derives from urinary bladder. 源自膀胱的永生细胞系细胞。 Yue Liu, Yongqun He immortal urinary bladder-derived cell line cell 永生的膀胱衍生细胞系细胞 An immortal cell line cell that derives from acetabular part of hip bone. 源自髋关节髋臼的永生细胞系细胞 Yue Liu, Yongqun He immortal acetabular part of hip bone-derived cell line cell 髋关节髋臼衍生细胞系细胞 An immortal cell line cell that derives from breast. 源自乳腺的永生细胞系细胞。 Yue Liu, Yongqun He immortal breast-derived cell line cell 永生的乳腺衍生细胞系细胞 An immortal cell line cell that derives from comma-shaped body. 源自逗点状体的永生细胞系细胞 Yue Liu, Yongqun He immortal comma-shaped body-derived cell line cell 永生的逗点状体衍生细胞系细胞 An immortal cell line cell that derives from supra-orbital vein. 源自眶上静脉的永生细胞系细胞。 Yue Liu, Yongqun He immortal supra-orbital vein-derived cell line cell 永生的眶上静脉衍生细胞系细胞 An immortal cell line cell that derives from uterus. 源自子宫的永生细胞系细胞。 Yue Liu, Yongqun He immortal uterus-derived cell line cell 永生的子宫衍生细胞系细胞 An immortal cell line cell that derives from uterine cervix. 源自子宫颈的永生衍生细胞系细胞 Yue Liu, Yongqun He immortal uterine cervix-derived cell line cell 永生的子宫颈衍生细胞系细胞 An immortal cell line cell that derives from pituitary gland. 源自垂体的永生细胞系细胞。 Yue Liu, Yongqun He immortal pituitary gland-derived cell line cell 永生的垂体衍生细胞系细胞 An immortal cell line cell that derives from eye. 源自眼的永生细胞系细胞。 Yue Liu, Yongqun He immortal eye-derived cell line cell 永生化的眼衍生细胞系细胞 An immortal plasma cell line cell that derives from organism part. 源自生物体部分的永生浆细胞系细胞。 Yue Liu, Yongqun He immortal organism part-derived plasma cell line cell 永生的生物部分衍生的浆细胞系细胞 An immortal cell line cell that derives from neural nucleus. 源自神经核的永生细胞系细胞。 Yue Liu, Yongqun He immortal neural nucleus-derived cell line cell 永生的神经核衍生细胞系细胞 An immortal cell line cell that derives from islet of Langerhans. 源自胰岛的永生细胞系细胞。 Yue Liu, Yongqun He immortal islet of Langerhans-derived cell line cell 永生的胰岛衍生细胞系细胞 a hybridoma cell line cell that is established by fusing cells from two mice. 通过融合来自两只小鼠的细胞建立的杂交瘤细胞系细胞。 most hybridoma cell line cells are mouse x mouse 大部分杂交瘤细胞是小鼠x小鼠 Yongqun He Yongqun He mouse x mouse hybridoma cell line cell 小鼠x小鼠杂交瘤细胞系细胞 An immortal epithelial cell line cell that derives from uterine cervix. 源自子宫颈的永生上皮细胞系细胞。 Yue Liu, Yongqun He immortal uterine cervix-derived epithelial cell line cell 永生的宫颈衍生上皮细胞系细胞 An immortal cell line cell that derives from ileocecal junction. 源自回盲连接的永生细胞系细胞。 Yue Liu, Yongqun He immortal ileocecal junction-derived cell line cell 永生的回盲连接衍生细胞系细胞 An immortal cell line cell that derives from larynx. 源自喉的永生细胞系细胞。 Yue Liu, Yongqun He immortal larynx-derived cell line cell 永生的喉衍生细胞系细胞 An immortal cell line cell that derives from esophagus. 源自食管的永生细胞系细胞。 Yue Liu, Yongqun He immortal esophagus-derived cell line cell 永生的食管衍生细胞系细胞 An immortal cell line cell that derives from round window. 源自圆窗的永生细胞系细胞。 Yue Liu, Yongqun He immortal round window-derived cell line cell 永生的圆窗衍生细胞系细胞 An immortal cell line cell that derives from intestine. 源自肠的永生细胞系细胞。 Yue Liu, Yongqun He immortal intestine-derived cell line cell 永生的肠衍生细胞系细胞 An immortal cell line cell that derives from posterior commissure. 源自后连合的永生细胞系细胞。 Yue Liu, Yongqun He immortal posterior commissure-derived cell line cell 永生的后连合衍生细胞系细胞 An immortal cell line cell that derives from adrenal gland. 源自肾上腺的永生细胞系细胞。 Yue Liu, Yongqun He immortal adrenal gland-derived cell line cell 永生的肾上腺衍生细胞系细胞 An immortal cell line cell that derives from aorta. 源自主动脉的永生细胞系细胞。 Yue Liu, Yongqun He immortal aorta-derived cell line cell 永生的主动脉衍生细胞系细胞 An immortal cell line cell that derives from prostate epithelium. 源自前列腺上皮的永生细胞系细胞。 Yue Liu, Yongqun He immortal prostate epithelium-derived cell line cell 永生的前列腺上皮衍生细胞系细胞 An immortal cell line cell that derives from spherical lensed eye. 源自球面透镜眼的永生细胞系细胞。 Yue Liu, Yongqun He immortal spherical lensed eye-derived cell line cell 永生的球面透镜眼衍生细胞系细胞 An immortal cell line cell that derives from connective tissue. 源自结缔组织的永生细胞系细胞。 Yue Liu, Yongqun He immortal connective tissue-derived cell line cell 永生的结缔组织衍生细胞系细胞 An immortal cell line cell that derives from anatomical cluster. 源自解剖簇的永生细胞系细胞。 Yue Liu, Yongqun He immortal anatomical cluster-derived cell line cell 永生的解剖簇衍生细胞系细胞 An immortal cell line cell that derives from chordate pharynx. 来自脊索动物咽的永生细胞系细胞。 Yue Liu, Yongqun He immortal chordate pharynx-derived cell line cell 永生的脊索动物咽衍生细胞系细胞 An immortal cell line cell that derives from retina. 源自视网膜的永生细胞系细胞。 Yue Liu, Yongqun He immortal retina-derived cell line cell 永生的视网膜衍生细胞系细胞 An immortal cell line cell that derives from nasal concha. 源自鼻甲的永生细胞系细胞。 Yue Liu, Yongqun He immortal nasal concha-derived cell line cell 永生的鼻甲衍生细胞系细胞 An immortal cell line cell that derives from trachea. 源自气管的永生细胞系细胞。 Yue Liu, Yongqun He immortal trachea-derived cell line cell 永生的气管衍生细胞系细胞 An immortal cell line cell that derives from renal glomerulus. 源自肾小球的永生细胞系细胞。 Yue Liu, Yongqun He immortal renal glomerulus-derived cell line cell 永生的肾小球衍生细胞系细胞 An immortal peripheral vein-derived B cell line cell that derives from human. 来自人的永生外周静脉衍生B细胞系细胞 Yue Liu, Yongqun He immortal human peripheral vein-derived B cell line cell 永生的人外周静脉衍生B细胞系细胞 An immortal cell line cell that derives from adrenal cortex. 源自肾上腺皮质的永生细胞系细胞。 Yue Liu, Yongqun He immortal adrenal cortex-derived cell line cell 永生的肾上腺皮质衍生细胞系细胞 An immortal peripheral vein-derived T cell line cell that derives from human. 来自人的永生外周静脉衍生T细胞系细胞 Yue Liu, Yongqun He immortal human peripheral vein-derived T cell line cell 永生的人外周静脉衍生T细胞系细胞 An immortal animal ovary-derived fibroblast cell line cell that derives from Chinese hamster. 源自中国仓鼠的永生动物卵巢衍生成纤维细胞系细胞。 Yue Liu, Yongqun He immortal Chinese hamster ovary-derived fibroblast cell line cell 永生的中国仓鼠卵巢衍生成纤维细胞系细胞 An immortal lung-derived fibroblast cell line cell that derives from human. 源自人的永生肺衍生成纤维细胞系细胞。 Yue Liu, Yongqun He immortal human lung-derived fibroblast cell line cell 永生化的人肺衍生成纤维细胞系细胞 An immortal skin-derived cell line cell that derives from human. 源自人的永生皮肤衍生的细胞系细胞。 Yue Liu, Yongqun He immortal human skin-derived cell line cell 永生化的人皮肤衍生细胞系细胞 An immortal organism part-derived cell line cell that derives from human. 源于人的永生生物部分衍生细胞系细胞。 Yue Liu, Yongqun He immortal human organism part-derived cell line cell 永生化的人生物部分衍生细胞系细胞 An immortal cell line cell that derives from cat. 源自猫的永生细胞系细胞。 Yue Liu, Yongqun He 永生的猫细胞系细胞 An immortal lymphocyte cell line cell that derives from human. 源自人的永生淋巴细胞系细胞。 Yue Liu, Yongqun He immortal human lymphocyte cell line cell 永生的人淋巴细胞系细胞 An immortal kidney-derived cell line cell that derives from cat. 源自猫的永生的肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal cat kidney-derived cell line cell 永生的猫肾衍生细胞系细胞 An immortal fibroblast cell line cell that derives from human. 源自人的永生成纤维细胞系细胞。 Yue Liu, Yongqun He immortal human fibroblast cell line cell 永生的人成纤维细胞系细胞 An immortal embryo-derived cell line cell that derives from cat. 源自猫的永生胚胎衍生的细胞系细胞。 Yue Liu, Yongqun He immortal cat embryo-derived cell line cell 永生的猫胚胎衍生细胞系细胞 An immortal septum of telencephalon-derived cell line cell that derives from human. 来自人的端脑中隔衍生细胞系细胞。 Yue Liu, Yongqun He immortal human septum of telencephalon-derived cell line cell 端脑衍生细胞系细胞的永生化人间隔 An immortal mixed endoderm/mesoderm-derived structure-derived cell line cell that derives from human. 来自人的永生混合内胚层/中胚层衍生结构衍生的细胞系细胞。 Yue Liu, Yongqun He immortal human mixed endoderm/mesoderm-derived structure-derived cell line cell 永生的人混合内胚层/中胚层衍生结构衍生的细胞系细胞 An immortal kidney-derived cell line cell that derives from monkey. 源自猴子的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal monkey kidney-derived cell line cell 永生化的猴肾衍生细胞系细胞 An immortal lung-derived cell line cell that derives from human. 源自人的永生肺衍生细胞系细胞。 Yue Liu, Yongqun He immortal human lung-derived cell line cell 永生的人肺衍生细胞系细胞 An immortal pair of lungs-derived cell line cell that derives from mouse. 源自小鼠的永生肺对衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse pair of lungs-derived cell line cell 永生的小鼠肺对衍生细胞系细胞 An immortal fibroblast cell line cell that derives from mouse. 源自小鼠的永生成纤维细胞系细胞。 Yue Liu, Yongqun He immortal mouse fibroblast cell line cell 永生的小鼠成纤维细胞系细胞 An immortal embryo-derived cell line cell that derives from mouse. 源自小鼠的永生胚胎衍生的细胞系细胞。 Yue Liu, Yongqun He immortal mouse embryo-derived cell line cell 永生的小鼠胚胎衍生细胞系细胞 An immortal cell line cell that derives from human. 源自人的永生细胞系细胞。 Yue Liu, Yongqun He immortal human cell line cell 永生的人细胞系细胞 An immortal liver-derived cell line cell that derives from rat. 源自大鼠的永生肝衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat liver-derived cell line cell 永生的大鼠肝衍生细胞系细胞 An immortal colon-derived cell line cell that derives from human. 源自人的永生结肠衍生细胞系细胞。 Yue Liu, Yongqun He immortal human colon-derived cell line cell 永生化的人结肠衍生细胞系细胞 An immortal caecum-derived cell line cell that derives from human. 源自人的永生盲肠衍生细胞系细胞。 Yue Liu, Yongqun He immortal human caecum-derived cell line cell 永生的人盲肠衍生细胞系细胞 An immortal brain-derived cell line cell that derives from human. 源自人的永生大脑衍生细胞系细胞。 Yue Liu, Yongqun He immortal human brain-derived cell line cell 永生的人大脑衍生细胞系细胞 An immortal skin-derived cell line cell that derives from mouse. 源自小鼠的永生皮肤衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse skin-derived cell line cell 永生的小鼠皮肤衍生细胞系细胞 An immortal collecting duct of renal tubule-derived cell line cell that derives from mouse. 源自小鼠的永生肾小管集合管衍生细胞系细胞 Yue Liu, Yongqun He immortal mouse collecting duct of renal tubule-derived cell line cell 永生的小鼠肾小管集合管衍生细胞系细胞 An immortal peripheral neuron cell line cell that derives from human. 源自人的永生外周神经元细胞系细胞。 Yue Liu, Yongqun He immortal human peripheral neuron cell line cell 永生的人外周神经元细胞系细胞 An immortal female gonad-derived cell line cell that derives from Chinese hamster. 源自中国仓鼠的永生雌性性腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal Chinese hamster female gonad-derived cell line cell 永生的中国仓鼠雌性性腺衍生细胞系细胞 An immortal cell line cell that derives from vervet monkey. 源自黑长尾猴的永生细胞系细胞。 Yue Liu, Yongqun He immortal vervet monkey cell line cell 永生的黑长尾猴细胞系细胞 An immortal kidney-derived cell line cell that derives from vervet monkey. 源自黑长尾猴的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal vervet monkey kidney-derived cell line cell 永生的黑长尾猴肾衍生细胞系细胞 An immortal mast cell line cell that derives from mouse. 源自小鼠的永生肥大细胞系细胞。 Yue Liu, Yongqun He immortal mouse mast cell line cell 永生的小鼠肥大细胞系细胞 An immortal liver-derived cell line cell that derives from mouse. 源自小鼠的永生肝衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse liver-derived cell line cell 永生的小鼠肝衍生细胞系细胞 An immortal embryo-derived cell line cell that derives from Drosophila melanogaster. 源自黑腹果蝇的永生胚胎衍生细胞系细胞。 Yue Liu, Yongqun He immortal Drosophila melanogaster embryo-derived cell line cell 永生的黑腹果蝇胚胎衍生细胞系细胞 An immortal fetal membrane-derived cell line cell that derives from human. 源自人的永生胎膜衍生细胞系细胞。 Yue Liu, Yongqun He immortal human fetal membrane-derived cell line cell 永生的人胎膜衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from mouse. 源自小鼠的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse kidney-derived cell line cell 永生的小鼠肾衍生细胞系细胞 An immortal testis-derived cell line cell that derives from mouse. 源自小鼠的永生睾丸衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse testis-derived cell line cell 永生的小鼠睾丸衍生细胞系细胞 An immortal stomach-derived cell line cell that derives from human. 源自人的永生胃衍生细胞系细胞。 Yue Liu, Yongqun He immortal human stomach-derived cell line cell 永生的人胃衍生细胞系细胞 An immortal pancreas-derived cell line cell that derives from human. 源自人的永生胰腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal human pancreas-derived cell line cell 永生的人胰腺衍生细胞系细胞 An immortal bone element-derived cell line cell that derives from human. 源自人的永生骨元素衍生细胞系细胞。 Yue Liu, Yongqun He immortal human bone element-derived cell line cell 永生的人骨元素衍生细胞系细胞 An immortal cell line cell that derives from rat. 源自大鼠的永生细胞系细胞。 Yue Liu, Yongqun He immortal rat cell line cell 永生的大鼠细胞系细胞 An immortal epithelial cell line cell that derives from human. 源自人的永生上皮细胞系细胞。 Yue Liu, Yongqun He immortal human epithelial cell line cell 永生的人上皮细胞系细胞 An immortal urinary bladder-derived cell line cell that derives from human. 源自人的永生膀胱衍生细胞系细胞。 Yue Liu, Yongqun He immortal human urinary bladder-derived cell line cell 永生的人膀胱衍生细胞系细胞 An immortal liver-derived cell line cell that derives from human. 源自人的永生肝衍生细胞系细胞。 Yue Liu, Yongqun He immortal human liver-derived cell line cell 永生的人肝衍生细胞系细胞 An immortal acetabular part of hip bone-derived cell line cell that derives from human. 源自人的永生髋骨髋臼衍生细胞系细胞 Yue Liu, Yongqun He immortal human acetabular part of hip bone-derived cell line cell 永生的人髋骨髋臼衍生细胞系细胞 An immortal fetal membrane-derived cell line cell that derives from rhesus macaque. 源自恒河猴的永生胎膜衍生细胞系细胞。 Yue Liu, Yongqun He immortal rhesus macaque fetal membrane-derived cell line cell 永生的恒河猴胎膜衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from dog. 源自狗的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal dog kidney-derived cell line cell 永生的狗肾衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from cattle. 源自牛的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal cattle kidney-derived cell line cell 永生的牛肾衍生细胞系细胞 An immortal comma-shaped body-derived cell line cell that derives from human. 源自人的永生逗点状体衍生细胞系细胞。 Yue Liu, Yongqun He immortal human comma-shaped body-derived cell line cell 永生的人逗点状体衍生细胞系细胞 An immortal breast-derived cell line cell that derives from human. 源自人的永生乳腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal human breast-derived cell line cell 永生的人乳腺衍生细胞系细胞 An immortal supra-orbital vein-derived cell line cell that derives from human. 源自人的永生眶上静脉衍生细胞系细胞。 Yue Liu, Yongqun He immortal human supra-orbital vein-derived cell line cell 永生的人眶上静脉衍生细胞系细胞 An immortal muscle cell line cell that derives from mouse. 源自小鼠的永生肌细胞系细胞。 Yue Liu, Yongqun He immortal mouse muscle cell line cell 永生的小鼠肌肉细胞系细胞 An immortal uterus-derived cell line cell that derives from human. 源自人的永生子宫衍生细胞系细胞。 Yue Liu, Yongqun He immortal human uterus-derived cell line cell 永生的人子宫衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from human. 源自人的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal human kidney-derived cell line cell 永生的人肾衍生细胞系细胞 An immortal pair of lungs-derived cell line cell that derives from Mustelinae. 源自鼬亚科的永生肺对衍生细胞系细胞。 Yue Liu, Yongqun He immortal Mustelinae pair of lungs-derived cell line cell 永生的鼬亚科肺对衍生细胞系细胞 An immortal brain-derived cell line cell that derives from mouse. 源自小鼠的永生大脑衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse brain-derived cell line cell 永生的小鼠大脑衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from pig. 源自猪的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal pig kidney-derived cell line cell 永生的猪肾衍生细胞系细胞 An immortal uterine cervix-derived cell line cell that derives from human. 源自人的永生子宫颈衍生细胞系细胞。 Yue Liu, Yongqun He immortal human uterine cervix-derived cell line cell 永生的子宫颈衍生细胞系细胞 An immortal pituitary gland-derived cell line cell that derives from rat. 源自大鼠的永生垂体腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat pituitary gland-derived cell line cell 永生的大鼠垂体衍生细胞系细胞 An immortal female gonad-derived cell line cell that derives from human. 源自人的永生雌性性腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal human female gonad-derived cell line cell 永生的人雌性性腺衍生细胞系细胞 An immortal thyroid gland-derived cell line cell that derives from human. 源自人的永生甲状腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal human thyroid gland-derived cell line cell 永生的人甲状腺衍生细胞系细胞 An immortal embryo-derived cell line cell that derives from chicken. 源自鸡的永生胚胎衍生细胞系细胞。 Yue Liu, Yongqun He immortal chicken embryo-derived cell line cell 永生的鸡胚胎衍生细胞系细胞 An immortal brain-derived cell line cell that derives from cat. 源自猫的永生大脑衍生细胞系细胞。 Yue Liu, Yongqun He immortal cat brain-derived cell line cell 永生的猫大脑衍生细胞系细胞 An immortal organism part-derived plasma cell line cell that derives from mouse. 源自小鼠的永生生物部分衍生浆细胞系细胞。 Yue Liu, Yongqun He immortal mouse organism part-derived plasma cell line cell 永生的小鼠生物部分衍生浆细胞系细胞 An immortal blood cell line cell that derives from human. 源自人的永生血细胞系细胞。 Yue Liu, Yongqun He immortal human blood cell line cell 永生的人血细胞系细胞 An immortal megakaryocyte-erythroid progenitor cell line cell that derives from mouse. 源自小鼠的衍生巨核细胞-红细胞祖细胞系细胞。 Yue Liu, Yongqun He immortal mouse megakaryocyte-erythroid progenitor cell line cell 永生的小鼠巨核细胞-红细胞祖细胞系细胞 An immortal neural nucleus-derived cell line cell that derives from rat. 源自大鼠的永生神经核衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat neural nucleus-derived cell line cell 永生的大鼠神经核衍生细胞系细胞 An immortal septum of telencephalon-derived cell line cell that derives from mouse. 源自小鼠的永生端脑中隔衍生细胞系细胞 Yue Liu, Yongqun He immortal mouse septum of telencephalon-derived cell line cell 永生的小鼠端脑中隔衍生细胞系细胞 An immortal islet of Langerhans-derived cell line cell that derives from mouse. 源自小鼠的永生胰岛衍生细胞系细胞 Yue Liu, Yongqun He immortal mouse islet of Langerhans-derived cell line cell 永生的小鼠胰岛衍生细胞系细胞 An immortal uterine cervix-derived epithelial cell line cell that derives from human. 源自人的永生子宫颈衍生上皮细胞系细胞。 Yue Liu, Yongqun He immortal human uterine cervix-derived epithelial cell line cell 永生的人宫颈衍生上皮细胞系细胞 An immortal ileocecal junction-derived cell line cell that derives from human. 源自人的永生回盲连接衍生细胞系细胞。 Yue Liu, Yongqun He immortal human ileocecal junction-derived cell line cell 永生的人回盲连接衍生细胞系细胞 An immortal septum of telencephalon-derived cell line cell that derives from rat. 来自大鼠的永生端脑中隔衍生细胞系细胞 Yue Liu, Yongqun He immortal rat septum of telencephalon-derived cell line cell 永生的大鼠端脑中隔衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from Syrian golden hamster. 来自叙利亚金黄仓鼠的肾衍生的细胞系细胞。 Yue Liu, Yongqun He immortal Syrian golden hamster kidney-derived cell line cell 永生的叙利亚金黄仓鼠肾衍生细胞系细胞 An immortal B cell line cell that derives from human. 源自人的永生B细胞系细胞。 Yue Liu, Yongqun He immortal human B-cell line cell 永生的人B-细胞系细胞 immortal human B cell line cell 永生的人B细胞系细胞 An immortal larynx-derived cell line cell that derives from human. 源自人的永生喉衍生细胞系细胞。 Yue Liu, Yongqun He immortal human larynx-derived cell line cell 永生的人喉衍生细胞系细胞 An immortal esophagus-derived cell line cell that derives from human. 源自人的永生食管衍生细胞系细胞。 Yue Liu, Yongqun He immortal human esophagus-derived cell line cell 永生的人食管衍生细胞系细胞 An immortal neuron cell line cell that derives from rat. 源自大鼠的永生神经元细胞系细胞。 Yue Liu, Yongqun He immortal rat neuron cell line cell 永生的大鼠神经元细胞系细胞 An immortal round window-derived cell line cell that derives from mouse. 源自小鼠的永生圆窗衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse round window-derived cell line cell 永生的小鼠圆窗衍生细胞系细胞 An immortal pancreas-derived cell line cell that derives from rat. 来自大鼠的永生胰腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat pancreas-derived cell line cell 永生的大鼠胰腺衍生细胞系细胞 An immortal intestine-derived cell line cell that derives from human. 源自人的永生肠衍生细胞系细胞。 Yue Liu, Yongqun He immortal human intestine-derived cell line cell 永生的人肠衍生细胞系细胞 An immortal dento-alveolar joint-derived cell line cell that derives from mouse. 源自小鼠的永生牙齿-牙槽骨关节衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse dento-alveolar joint-derived cell line cell 永生的小鼠牙齿-牙槽骨关节衍生细胞系细胞 An immortal posterior commissure-derived cell line cell that derives from zebra fish. 来自斑马鱼的永生后连合衍生细胞系细胞。 Yue Liu, Yongqun He immortal zebra fish posterior commissure-derived cell line cell 永生的斑马鱼后连合衍生细胞系细胞 An immortal adrenal gland-derived cell line cell that derives from human. 源自人的永生肾上腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal human adrenal gland-derived cell line cell 永生的人肾上腺衍生细胞系细胞 An immortal blood cell line cell that derives from mouse. 源自小鼠的永生血细胞系细胞。 Yue Liu, Yongqun He immortal mouse blood cell line cell 永生的小鼠血细胞系细胞 An immortal placenta-derived cell line cell that derives from human. 源自人的永生胎盘衍生细胞系细胞。 Yue Liu, Yongqun He immortal human placenta-derived cell line cell 永生的人胎盘衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from rhesus macaque. 源自恒河猴的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal rhesus macaque kidney-derived cell line cell 永生的恒河猴肾衍生细胞系细胞 An immortal monocyte cell line cell that derives from mouse. 源自小鼠的永生单核细胞系细胞。 Yue Liu, Yongqun He immortal mouse monocyte cell line cell 永生的小鼠单核细胞系细胞 An immortal round window-derived cell line cell that derives from Mus musculus x Rattus norvegicus. 来源于小家鼠x褐家鼠的永生圆窗衍生细胞系细胞。 Yue Liu, Yongqun He immortal Mus musculus x Rattus norvegicus round window-derived cell line cell 永生的小家鼠x褐家鼠圆窗衍生细胞系细胞 An immortal pair of lungs-derived cell line cell that derives from rat. 源自大鼠的永生肺对衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat pair of lungs-derived cell line cell 永生的大鼠肺对衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from rat. 源自大鼠的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat kidney-derived cell line cell 永生的大鼠肾衍生细胞系细胞 An immortal macrophage cell line cell that derives from mouse. 源自小鼠的永生巨噬细胞系细胞。 Yue Liu, Yongqun He immortal mouse macrophage cell line cell 永生的小鼠巨噬细胞系细胞 An immortal septum of telencephalon-derived cell line cell that derives from American opossum. 源自美国负鼠的永生端脑中隔衍生细胞系细胞 Yue Liu, Yongqun He immortal American opossum septum of telencephalon-derived cell line cell 永生的北美负鼠端脑中隔衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from European rabbit, rabbit. 源自欧洲兔子、家兔的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal European rabbit, rabbit kidney-derived cell line cell 永生的欧洲兔、家兔肾脏衍生细胞系细胞 An immortal prostate gland-derived cell line cell that derives from human. 源自人的永生前列腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal human prostate gland-derived cell line cell 永生的人前列腺衍生细胞系细胞 An immortal female gonad-derived cell line cell that derives from northern pike. 源自白斑狗鱼的永生雌性性腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal northern pike female gonad-derived cell line cell 永生的白斑狗鱼雌性性腺衍生细胞系细胞 An immortal adrenal gland-derived cell line cell that derives from rat. 源自大鼠的永生肾上腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat adrenal gland-derived cell line cell 永生的大鼠肾上腺衍生细胞系细胞 An immortal kidney-derived cell line cell that derives from Potoroo. 源自长鼻袋鼠的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal Potoroo kidney-derived cell line cell 永生的长鼻袋鼠肾衍生细胞系细胞 An immortal pair of lungs-derived cell line cell that derives from Chinese hamster. 源自中国仓鼠的永生肺对衍生细胞系细胞。 Yue Liu, Yongqun He immortal Chinese hamster pair of lungs-derived cell line cell 永生的中国仓鼠肺对衍生细胞系细胞 An immortal prostate epithelium-derived cell line cell that derives from human. 源自人的永生前列腺上皮细胞系细胞。 Yue Liu, Yongqun He immortal human prostate epithelium-derived cell line cell 永生的人前列腺上皮衍生细胞系细胞 An immortal spherical lensed eye-derived cell line cell that derives from human. 源自人类的永生球面透镜眼衍生细胞系细胞。 Yue Liu, Yongqun He immortal human spherical lensed eye-derived cell line cell 永生的人球面透镜眼衍生细胞系细胞 An immortal aorta-derived cell line cell that derives from rat. 源自大鼠的永生主动脉衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat aorta-derived cell line cell 永生的大鼠主动脉衍生细胞系细胞 An immortal vasculature-derived cell line cell that derives from human. 源自人的永生血系统衍生的细胞系细胞。 Yue Liu, Yongqun He immortal human vasculature-derived cell line cell 永生的人血管衍生细胞系细胞 An immortal connective tissue-derived cell line cell that derives from human. 源自人的永生结缔组织细胞系细胞。 Yue Liu, Yongqun He immortal human connective tissue-derived cell line cell 永生的人结缔组织衍生细胞系细胞 An immortal eye-derived cell line cell that derives from human. 源自人的永生眼衍生细胞系细胞。 Yue Liu, Yongqun He immortal human eye-derived cell line cell 永生的人眼衍生细胞系细胞 An immortal anatomical cluster-derived cell line cell that derives from human. 源自人类的永生解剖簇衍生细胞系细胞。 Yue Liu, Yongqun He immortal human anatomical cluster-derived cell line cell 永生的人解剖簇衍生细胞系细胞 An immortal chordate pharynx-derived cell line cell that derives from human. 源自人的永生脊索动物咽衍生细胞系细胞。 Yue Liu, Yongqun He immortal human chordate pharynx-derived cell line cell 永生的人脊索动物咽衍生细胞系细胞 An immortal retina-derived cell line cell that derives from human. 源自人的永生视网膜衍生细胞系细胞。 Yue Liu, Yongqun He immortal human retina-derived cell line cell 永生的人视网膜衍生细胞系细胞 An immortal nasal concha-derived cell line cell that derives from cattle. 源自牛的永生鼻甲衍生细胞系细胞。 Yue Liu, Yongqun He immortal cattle nasal concha-derived cell line cell 永生的牛鼻甲衍生细胞系细胞 An immortal epithelial cell line cell that derives from rat. 源自大鼠的永生上皮细胞系细胞。 Yue Liu, Yongqun He immortal rat epithelial cell line cell 永生的大鼠上皮细胞系细胞 An immortal tongue-derived cell line cell that derives from human. 源自人的永生舌衍生细胞系细胞。 Yue Liu, Yongqun He immortal human tongue-derived cell line cell 永生的人舌衍生细胞系细胞 An immortal thymus-derived cell line cell that derives from dog. 源自狗的永生胸腺衍生的细胞系细胞。 Yue Liu, Yongqun He immortal dog thymus-derived cell line cell 永生的狗胸腺衍生细胞系细胞 An immortal aorta-derived cell line cell that derives from human. 源自人的永生主动脉衍生细胞系细胞。 Yue Liu, Yongqun He immortal human aorta-derived cell line cell 永生的人主动脉衍生细胞系细胞 An immortal testis-derived cell line cell that derives from pig. 源自猪的永生睾丸衍生的细胞系细胞。 Yue Liu, Yongqun He immortal pig testis-derived cell line cell 永生的猪睾丸衍生细胞系细胞 An immortal renal glomerulus-derived cell line cell that derives from mouse. 源自小鼠的永生肾小球衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse renal glomerulus-derived cell line cell 永生的小鼠肾小球衍生细胞系细胞 An immortal bone element-derived cell line cell that derives from rat. 源自大鼠的永生骨元素衍生细胞系细胞。 Yue Liu, Yongqun He immortal rat bone element-derived cell line cell 永生的大鼠骨元素衍生细胞系细胞 An immortal monocyte cell line cell that derives from human. 源自人的永生单核细胞系细胞。 Yue Liu, Yongqun He immortal human monocyte cell line cell 永生的人单核细胞系细胞 An immortal cell line cell that derives from Chinese hamster. 源自中国仓鼠的永生细胞系细胞。 Yue Liu, Yongqun He immortal Chinese hamster cell line cell 永生的中国仓鼠细胞系细胞 An immortal liver-derived cell line cell that derives from woodchuck. 源自土拨鼠的永生肝衍生细胞系细胞。 Yue Liu, Yongqun He immortal woodchuck liver-derived cell line cell 永生的土拨鼠肝衍生细胞系细胞 An immortal cell line cell that derives from cabbage looper. 源自粉纹夜蛾的永生细胞系细胞。 Yue Liu, Yongqun He immortal cabbage looper cell line cell 永生的粉纹夜蛾细胞系细胞 An immortal trachea-derived cell line cell that derives from cattle. 源自牛的永生气管衍生细胞系细胞。 Yue Liu, Yongqun He immortal cattle trachea-derived cell line cell 永生的牛气管衍生细胞系细胞 An immortal macrophage cell line cell that derives from dog. 源自狗的永生巨噬细胞系细胞。 Yue Liu, Yongqun He immortal dog macrophage cell line cell 永生的狗巨噬细胞系细胞 An immortal colon-derived cell line cell that derives from mouse. 源自小鼠的永生结肠衍生细胞系细胞。 Yue Liu, Yongqun He immortal mouse colon-derived cell line cell 永生的小鼠结肠衍生细胞系细胞 An immortal septum of telencephalon-derived cell line cell that derives from cat. 源自猫的永生端脑中隔衍生细胞系细胞。 Yue Liu, Yongqun He immortal cat septum of telencephalon-derived cell line cell 永生的猫端脑中隔衍生细胞系细胞 1301 1301 1301 cell 1301 细胞 HyperCLDB: cl5401 HyperCLDB: cl5401 4647 4647 4647 cell 4647 细胞 HyperCLDB: cl97 HyperCLDB: cl97 104C1 104C1 104C1 cell 104C1 细胞 ATCC: CRL-1405 ATCC: CRL-1405 10F7MN 10F7MN disease: hybridoma 疾病：杂交瘤 10F7MN cell 10F7MN 细胞 ATCC: HB-8162 ATCC: HB-8162 1116NS-3d 1116NS-3d disease: hybridoma 疾病：杂交瘤 1116NS-3d cell 1116NS-3d 细胞 ATCC: CRL-8019 ATCC: CRL-8019 143 B 143 B 143 B cell 143 B 细胞 HyperCLDB: cl10 HyperCLDB: cl10 LCL-1417 LCL-1417 143B 143B 143B cell 143B 细胞 ATCC: CRL-8303 ATCC: CRL-8303 CHEMBL: CHEMBL3307382 CHEMBL: CHEMBL3307382 CVCL: CVCL_2270 CVCL: CVCL_2270 145-2C11 145-2C11 145-2C11 cell 145-2C11 细胞 ATCC: CRL-1975 ATCC: CRL-1975 15P-1 15P-1 15P-1 cell 15P-1 细胞 ATCC: CRL-2618 ATCC: CRL-2618 1H10-6 1H10-6 1H10-6 cell 1H10-6 细胞 ATCC: HB-48 ATCC: HB-48 2.43 2.43 2.43 cell 2.43 细胞 ATCC: TIB-210 ATCC: TIB-210 225 225 225 cell 225 细胞 ATCC: HB-8508 ATCC: HB-8508 LCL-1145 LCL-1145 22RV1 22RV1 22RV1 cell 22RV1 细胞 ATCC: CRL-2505 ATCC: CRL-2505 HyperCLDB: cl7072 HyperCLDB: cl7072 22Rv1 22Rv1 22Rv1 cell 22Rv1 细胞 ATCC: CRL-2505 ATCC: CRL-2505 293 293 293 HEK 293 HEK 293 cell 293 细胞 HEK 293 HEK 293 HEK-293 HEK-293 WEB: http://hek293.com/ WEB: http://hek293.com/ WEB: http://web.expasy.org/cellosaurus/CVCL_0045 WEB: http://web.expasy.org/cellosaurus/CVCL_0045 WEB: https://www.atcc.org/Products/All/CRL-1573.aspx WEB: https://www.atcc.org/Products/All/CRL-1573.aspx Human Embryonic Kidney 293 人胚肾 293 HEK293 HEK293 cell ATCC: CRL-1573 ATCC: CRL-1573 BTO: BTO_0000007 EFO: EFO_0001182) RRID: CVCL_0045 RRID: CVCL_0045 293 c18 293 c18 WEB: https://www.atcc.org/Products/All/CRL-10852.aspx#characteristics WEB: https://www.atcc.org/Products/All/CRL-10852.aspx#characteristics 293 c18 cell 293 c18 细胞 ATCC: CRL-10852 ATCC: CRL-10852 293T/17 293T/17 WEB: https://www.atcc.org/Products/All/CRL-11268.aspx WEB: https://www.atcc.org/Products/All/CRL-11268.aspx 293T/17 cell 293T/17 细胞 ATCC: CRL-11268 ATCC: CRL-11268 LCL-2015 LCL-2015 2B8 2B8 2B8 cell 2B8 细胞 2D4 2D4 disease: hybridoma 疾病：杂交瘤 2D4 cell 2D4 细胞 ATCC: TIB-185 ATCC: TIB-185 2F7 2F7 2F7 cell 2F7 细胞 ATCC: CRL-10237 ATCC: CRL-10237 2V6.11 2V6.11 2V6.11 cell 2V6.11 细胞 ATCC: CRL-2784 ATCC: CRL-2784 31E9 31E9 disease: produces GIF 疾病：产生GIF 31E9 cell 31E9 细胞 ATCC: HB-11052 ATCC: HB-11052 35.1 35.1 disease: hybridoma 疾病：杂交瘤 35.1 cell 35.1 细胞 ATCC: HB-222 ATCC: HB-222 3T3 3T3 3T3 cell 3T3 细胞 HyperCLDB: cl65 HyperCLDB: cl65 MeSH: D016475 MeSH: D016475 3T3 clone A31 3T3 clone A31 3T3 clone A31 cell 3T3 clone A31 细胞 HyperCLDB: cl389 HyperCLDB: cl389 3T3 NIH TK- 3T3 NIH TK- 3T3 NIH TK- cell 3T3 NIH TK- 细胞 HyperCLDB: cl68 HyperCLDB: cl68 3T3-L1 3T3-L1 3T3-L1 cell 3T3-L1 细胞 HyperCLDB: cl71 HyperCLDB: cl71 HyperCLDB: cl72 HyperCLDB: cl72 MeSH: D041721 MeSH: D041721 3T3-Swiss albino 3T3-Swiss albino disease: feeder layer cell 疾病：饲养层细胞 3T3-Swiss albino cell 3T3-Swiss albino 细胞 ATCC: CCL-92 ATCC: CCL-92 HyperCLDB: cl83 HyperCLDB: cl83 HyperCLDB: cl86 HyperCLDB: cl86 3T6-Swiss albino 3T6-Swiss albino 3T6-Swiss albino cell 3T6-Swiss albino 细胞 ATCC: CCL-96 ATCC: CCL-96 HyperCLDB: cl89 HyperCLDB: cl89 4C9 4C9 disease: hybridoma 疾病：杂交瘤 4C9 cell 4C9 细胞 ATCC: CRL-2437 ATCC: CRL-2437 4D11 4D11 4D11 cell 4D11 细胞 ATCC: HB-240 ATCC: HB-240 4E11 4E11 disease: hybridoma 疾病：杂交瘤 4E11 cell 4E11 细胞 ATCC: HB-9259 ATCC: HB-9259 4F2C13 4F2C13 disease: hybridoma 疾病：杂交瘤 4F2C13 cell 4F2C13 细胞 ATCC: HB-22 ATCC: HB-22 4T1 4T1 4T1 cell 4T1 细胞 ATCC: CRL-2539 ATCC: CRL-2539 51.1 51.1 disease: hybridoma 疾病：杂交瘤 51.1 cell 51.1 细胞 ATCC: HB-230 ATCC: HB-230 53-7.313 53-7.313 53-7.313 cell 53-7.313 细胞 ATCC: TIB-104 ATCC: TIB-104 LCL-1702 LCL-1702 5637 5637 5637 cell 5637 细胞 ATCC: HTB-9 ATCC: HTB-9 CHEMBL: CHEMBL3308352 CHEMBL: CHEMBL3308352 CVCL: CVCL_0126 CVCL: CVCL_0126 EFO: EFO_0002096 EFO: EFO_0002096 5D5.11.6 5D5.11.6 disease: hybridoma 疾病：杂交瘤 5D5.11.6 cell 5D5.11.6 细胞 ATCC: HB-11895 ATCC: HB-11895 LCL-1772 LCL-1772 769-P 769-P 769-P cell 769-P 细胞 ATCC: CRL-1933 ATCC: CRL-1933 CHEMBL: CHEMBL3308705 CHEMBL: CHEMBL3308705 CVCL: CVCL_1050 CVCL: CVCL_1050 EFO: EFO_0002099 EFO: EFO_0002099 LCL-1773 LCL-1773 786-O 786-O 786-O cell 786-O 细胞 ATCC: CRL-1932 ATCC: CRL-1932 7G7B6 7G7B6 7G7B6 cell 7G7B6 细胞 ATCC: HB-8784 ATCC: HB-8784 8E5 8E5 8E5 cell 8E5 细胞 ATCC: CRL-8993 ATCC: CRL-8993 A 375 A 375 A 375 cell A 375 细胞 HyperCLDB: cl198 HyperCLDB: cl198 LCL-1348 LCL-1348 ZN ZN A172 A172 A172 cell A172 细胞 ATCC: CRL-1620 ATCC: CRL-1620 LCL-1409 LCL-1409 ZN ZN A-204 A-204 A-204 cell A-204 细胞 ATCC: HTB-82 ATCC: HTB-82 CHEMBL: CHEMBL3308354 CHEMBL: CHEMBL3308354 CVCL: CVCL_1058 CVCL: CVCL_1058 EFO: EFO_0002102 EFO: EFO_0002102 LCL-1518 LCL-1518 A2780 A2780 A2780 cell A2780 细胞 CHEMBL: CHEMBL3308421 CHEMBL: CHEMBL3308421 CVCL: CVCL_0134 CVCL: CVCL_0134 ECACC: 93112519 ECACC: 93112519 HyperCLDB: cl193 HyperCLDB: cl193 LCL-1026 LCL-1026 A3 A3 A3 cell A3 细胞 ATCC: CRL-2570 ATCC: CRL-2570 A375 A375 Comment: this term is obsolete because it is duplicated with another term 'A-375 cell' (CLO_0001582). -- Oliver He 评论：这个术语是过时的，因为它与另一个术语“A-375细胞”（CLO_0001582）重复。 -- Oliver He obsolete: A375 cell 过时的: A375 细胞 HyperCLDB: cl199 HyperCLDB: cl199 LCL-1235 LCL-1235 A-375 A-375 WEB: http://bioinformatics.hsanmartino.it/hypercldb/cl199.html WEB: http://bioinformatics.hsanmartino.it/hypercldb/cl199.html A375 A375 A375 cell A375 细胞 disease: malignant melanoma 疾病：恶性黑素瘤 A-375 cell A-375 细胞 ATCC: CRL-1619 ATCC: CRL-1619 CHEMBL: CHEMBL3308077 CHEMBL: CHEMBL3308077 CVCL: CVCL_0132 CVCL: CVCL_0132 EFO: EFO_0002103 EFO: EFO_0002103 HyperCLDB: cl199 HyperCLDB: cl199 LCL-1600 LCL-1600 A-427 A-427 A-427 cell A-427 细胞 ATCC: HTB-53 ATCC: HTB-53 CHEMBL: CHEMBL3307775 CHEMBL: CHEMBL3307775 CVCL: CVCL_1055 CVCL: CVCL_1055 EFO: EFO_0002104 EFO: EFO_0002104 LCL-1404 LCL-1404 ZN ZN A431 A431 A431 cell A431 细胞 ECACC: 85090402 ECACC: 85090402 HyperCLDB: cl160 HyperCLDB: cl160 A-431 A-431 A-431 cell A-431 细胞 ATCC: CRL-1555 ATCC: CRL-1555 LCL-1601 LCL-1601 A549 A549 A549 cell A549 细胞 ATCC: CCL-185 ATCC: CCL-185 CHEMBL: CHEMBL3307651 CHEMBL: CHEMBL3307651 CVCL: CVCL_0023 CVCL: CVCL_0023 EFO: EFO_0001086 EFO: EFO_0001086 HyperCLDB: cl207 HyperCLDB: cl207 A6 A6 A6 cell A6 细胞 ATCC: CRL-8192 ATCC: CRL-8192 A-673 A-673 A-673 cell A-673 细胞 ATCC: CRL-1598 ATCC: CRL-1598 A7r5 A7r5 A7r5 cell A7r5 细胞 ATCC: CRL-1444 ATCC: CRL-1444 A-9 A-9 A-9 cell A-9 细胞 ATCC: CRL-6319 ATCC: CRL-6319 AB.9 AB.9 AB.9 cell AB.9 细胞 ATCC: CRL-2298 ATCC: CRL-2298 LCL-1756 LCL-1756 ACHN ACHN ACHN cell ACHN 细胞 ATCC: CRL-1611 ATCC: CRL-1611 CHEMBL: CHEMBL3307633 CHEMBL: CHEMBL3307633 CVCL: CVCL_1067 CVCL: CVCL_1067 EFO: EFO_0002108 EFO: EFO_0002108 AE-1 AE-1 disease: hybridoma 疾病：杂交瘤 AE-1 cell AE-1 细胞 ATCC: HB-72 ATCC: HB-72 AE-2 AE-2 AE-2 cell AE-2 细胞 ATCC: HB-73 ATCC: HB-73 Aedes albopictus 白纹伊蚊 Aedes albopictus cell 白纹伊蚊细胞 ATCC: CCL-126 ATCC: CCL-126 Aedes albopictus clone C6/36 白纹伊蚊克隆 C6/36 Aedes albopictus clone C6/36 cell 白纹伊蚊C6/36细胞 ATCC: CRL-1660 ATCC: CRL-1660 LCL-1893 LCL-1893 AGS AGS AGS cell AGS 细胞 ATCC: CRL-1739 ATCC: CRL-1739 CHEMBL: CHEMBL3308078 CHEMBL: CHEMBL3308078 CVCL: CVCL_0139 CVCL: CVCL_0139 EFO: EFO_0002109 EFO: EFO_0002109 AML-193 AML-193 AML-193 cell AML-193 细胞 ATCC: CRL-9589 ATCC: CRL-9589 HyperCLDB: cl272 HyperCLDB: cl272 AR42J AR42J disease: tumor 疾病：肿瘤 AR42J cell AR42J 细胞 ATCC: CRL-1492 ATCC: CRL-1492 LCL-1730 LCL-1730 AsPC-1 AsPC-1 AsPC-1 cell AsPC-1 细胞 ATCC: CRL-1682 ATCC: CRL-1682 CHEMBL: CHEMBL3307994 CHEMBL: CHEMBL3307994 CVCL: CVCL_0152 CVCL: CVCL_0152 EFO: EFO_0002112 EFO: EFO_0002112 AtT-20 AtT-20 AtT-20 cell AtT-20 细胞 ATCC: CCL-89 ATCC: CCL-89 B16-F0 B16-F0 B16-F0 cell B16-F0 细胞 ATCC: CRL-6322 ATCC: CRL-6322 B16-F1 B16-F1 B16-F1 cell B16-F1 细胞 ATCC: CRL-6323 ATCC: CRL-6323 B16-F10 B16-F10 B16-F10 cell B16-F10 细胞 ATCC: CRL-6475 ATCC: CRL-6475 B82 B82 B82 cell B82 细胞 HyperCLDB: cl356 HyperCLDB: cl356 B95-8 B95-8 B95-8 cell B95-8 细胞 HyperCLDB: cl366 HyperCLDB: cl366 HyperCLDB: cl368 HyperCLDB: cl368 BA/F3 BA/F3 BA/F3 cell BA/F3 细胞 HyperCLDB: cl5028 HyperCLDB: cl5028 BALB/3T3 clone A31 BALB/3T3 clone A31 BALB/3T3 clone A31 cell BALB/3T3 clone A31 细胞 ATCC: CCL-163 ATCC: CCL-163 HyperCLDB: cl386 HyperCLDB: cl386 MeSH: D041702 MeSH: D041702 BB7.2 BB7.2 BB7.2 cell BB7.2 细胞 ATCC: HB-82 ATCC: HB-82 BC3H1 BC3H1 disease: methylnitrosourea-induced smooth muscle-like tumor 疾病：甲基亚硝脲诱导的平滑肌肿瘤 BC3H1 cell BC3H1 细胞 ATCC: CRL-1443 ATCC: CRL-1443 LCL-1972 LCL-1972 BE(2)-M17 BE(2)-M17 BE(2)-M17 cell BE(2)-M17 细胞 ATCC: CRL-2267 ATCC: CRL-2267 BEAS-2B BEAS-2B disease: virus transformed 疾病：病毒转化 BEAS-2B cell BEAS-2B 细胞 ATCC: CRL-9609 ATCC: CRL-9609 Beta-TC-6 Beta-TC-6 Beta-TC-6 cell Beta-TC-6 细胞 ATCC: CRL-11506 ATCC: CRL-11506 BEWO BEWO BEWO cell BEWO 细胞 HyperCLDB: cl7082 HyperCLDB: cl7082 BF-11 BF-11 BF-11 cell BF-11 细胞 ATCC: CRL-8164 ATCC: CRL-8164 BGC-823 BGC-823 BGC-823 cell BGC-823 细胞 HyperCLDB: cl4930 HyperCLDB: cl4930 BHK-21 BHK-21 BHK-21 cell BHK-21 细胞 HyperCLDB: cl448 HyperCLDB: cl448 BNL 1ME A.7R.1 BNL 1ME A.7R.1 disease: chemically transformed 疾病：化学转化 BNL 1ME A.7R.1 cell BNL 1ME A.7R.1 细胞 ATCC: TIB-75 ATCC: TIB-75 BNL CL.2 BNL CL.2 disease: embryonic 疾病：胚胎 BNL CL.2 cell BNL CL.2 细胞 ATCC: TIB-73 ATCC: TIB-73 BRL 3A BRL 3A BRL 3A cell BRL 3A 细胞 ATCC: CRL-1442 ATCC: CRL-1442 BS-C-1 BS-C-1 BS-C-1 cell BS-C-1 细胞 ATCC: CCL-26 ATCC: CCL-26 BT BT BT cell BT 细胞 ATCC: CRL-1390 ATCC: CRL-1390 LCL-1476 LCL-1476 ZN ZN BT-20 BT-20 BT20 BT20 BT-20 cell BT-20 细胞 ATCC: HTB-19 ATCC: HTB-19 CHEMBL: CHEMBL3307766 CHEMBL: CHEMBL3307766 CVCL: CVCL_0178 CVCL: CVCL_0178 EFO: EFO_0001092 EFO: EFO_0001092 LCL-1308 LCL-1308 BT-474 BT-474 disease: ductal carcinoma 疾病：导管癌 BT-474 cell BT-474 细胞 ATCC: HTB-20 ATCC: HTB-20 CHEMBL: CHEMBL3307636 CHEMBL: CHEMBL3307636 CVCL: CVCL_0179 CVCL: CVCL_0179 EFO: EFO_0001093 EFO: EFO_0001093 LCL-1310 LCL-1310 BT-549 BT-549 disease: ductal carcinoma 疾病：导管癌 BT-549 cell BT-549 细胞 ATCC: HTB-122 ATCC: HTB-122 CHEMBL: CHEMBL3308490 CHEMBL: CHEMBL3308490 CVCL: CVCL_1092 CVCL: CVCL_1092 EFO: EFO_0001096 EFO: EFO_0001096 BW5147 BW5147 BW5147 cell BW5147 细胞 HyperCLDB: cl512 HyperCLDB: cl512 LCL-1731 LCL-1731 BxPC-3 BxPC-3 BxPC-3 cell BxPC-3 细胞 ATCC: CRL-1687 ATCC: CRL-1687 CHEMBL: CHEMBL3307637 CHEMBL: CHEMBL3307637 CVCL: CVCL_0186 CVCL: CVCL_0186 EFO: EFO_0002709 EFO: EFO_0002709 HyperCLDB: cl516 HyperCLDB: cl516 HyperCLDB: cl518 HyperCLDB: cl518 c1 (B6NLxv1c2) c1 (B6NLxv1c2) c1 (B6NLxv1c2) cell c1 (B6NLxv1c2) 细胞 ATCC: CRL-2716 ATCC: CRL-2716 C127 C127 C127 cell C127 细胞 HyperCLDB: cl546 HyperCLDB: cl546 C2 C2 disease: hepatoma 疾病：肝癌 C2 cell C2 细胞 HyperCLDB: cl559 HyperCLDB: cl559 LCL-1300 LCL-1300 C-33 A C-33 A C-33 A cell C-33 A 细胞 ATCC: HTB-31 ATCC: HTB-31 C3H/10T1/2, Clone 8 C3H/10T1/2, Clone 8 C3H/10T1/2, Clone 8 cell C3H/10T1/2, Clone 8 细胞 ATCC: CCL-226 ATCC: CCL-226 HyperCLDB: cl567 HyperCLDB: cl567 C6 C6 disease: glial tumor 疾病：胶质瘤 C6 cell C6 细胞 HyperCLDB: cl581 HyperCLDB: cl581 LCL-1011 LCL-1011 C8166 C8166 C8166 cell C8166 细胞 CHEMBL: CHEMBL3307638 CHEMBL: CHEMBL3307638 CVCL: CVCL_1099 CVCL: CVCL_1099 ECACC: 88051601 ECACC: 88051601 HyperCLDB: cl589 HyperCLDB: cl589 C8-D1A C8-D1A C8-D1A cell C8-D1A 细胞 ATCC: CRL-2541 ATCC: CRL-2541 LCL-1541 LCL-1541 ZN ZN Ca Ski Ca Ski Ca Ski cell Ca Ski 细胞 ATCC: CRL-1550 ATCC: CRL-1550 ECACC: 87020501 ECACC: 87020501 LCL-2017 LCL-2017 CA46 CA46 disease: Burkitts lymphoma 疾病：伯基特淋巴瘤 CA46 cell CA46 细胞 ATCC: CRL-1648 ATCC: CRL-1648 CHEMBL: CHEMBL3308488 CHEMBL: CHEMBL3308488 CVCL: CVCL_1101 CVCL: CVCL_1101 EFO: EFO_0002124 EFO: EFO_0002124 HyperCLDB: cl609 HyperCLDB: cl609 LCL-1170 LCL-1170 Caco-2 Caco-2 disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 Caco-2 cell Caco-2 细胞 ATCC: HTB-37 ATCC: HTB-37 CHEMBL: CHEMBL3307519 CHEMBL: CHEMBL3307519 CVCL: CVCL_0025 CVCL: CVCL_0025 EFO: EFO_0001099 EFO: EFO_0001099 HyperCLDB: cl618 HyperCLDB: cl618 MeSH: D018938 MeSH: D018938 CAKI-1 CAKI-1 CAKI-1 cell CAKI-1 细胞 HyperCLDB: cl623 HyperCLDB: cl623 Caki-2 Caki-2 Caki-2 cell Caki-2 细胞 ATCC: HTB-47 ATCC: HTB-47 HyperCLDB: cl626 HyperCLDB: cl626 HyperCLDB: cl627 HyperCLDB: cl627 LCL-1215 LCL-1215 CAL 27 CAL 27 CAL 27 cell CAL 27 细胞 ATCC: CRL-2095 ATCC: CRL-2095 CAL-27 CAL-27 CAL-27 cell CAL-27 细胞 HyperCLDB: cl7086 HyperCLDB: cl7086 LCL-1580 LCL-1580 ZN ZN Calu-1 Calu-1 disease: epidermoid carcinoma (lung primary) 疾病：表皮样癌（肺原发） Calu-1 cell Calu-1 细胞 ATCC: HTB-54 ATCC: HTB-54 CHEMBL: CHEMBL3308031 CHEMBL: CHEMBL3308031 CVCL: CVCL_0608 CVCL: CVCL_0608 EFO: EFO_0002151 EFO: EFO_0002151 HyperCLDB: cl631 HyperCLDB: cl631 HyperCLDB: cl632 HyperCLDB: cl632 LCL-1631 LCL-1631 ZN ZN Calu-3 Calu-3 Calu-3 cell Calu-3 细胞 ATCC: HTB-55 ATCC: HTB-55 CHEMBL: CHEMBL3307390 CHEMBL: CHEMBL3307390 CVCL: CVCL_0609 CVCL: CVCL_0609 EFO: EFO_0002819 EFO: EFO_0002819 LCL-1626 LCL-1626 ZN ZN Calu-6 Calu-6 disease: unknown, probably lung anaplastic carcinoma 疾病：未知，可能是肺间变性癌 Calu-6 cell Calu-6 细胞 ATCC: HTB-56 ATCC: HTB-56 CHEMBL: CHEMBL3307639 CHEMBL: CHEMBL3307639 CVCL: CVCL_0236 CVCL: CVCL_0236 EFO: EFO_0002152 EFO: EFO_0002152 Sanger: COSMIC ID:724859 Sanger: COSMIC ID:724859 LCL-1519 LCL-1519 ZN ZN Caov-3 Caov-3 Caov-3 cell Caov-3 细胞 ATCC: HTB-75 ATCC: HTB-75 LCL-1520 LCL-1520 ZN ZN Caov-4 Caov-4 Caov-4 cell Caov-4 细胞 CHEMBL: CHEMBL3308232 CHEMBL: CHEMBL3308232 CVCL: CVCL_0202 CVCL: CVCL_0202 CaSki CaSki disease: carcinoma, epidermoid, from metastatic site 疾病：转移部位的上皮癌，表皮样癌 CaSki cell CaSki 细胞 HyperCLDB: cl4940 HyperCLDB: cl4940 CC81 CC81 CC81 cell CC81 细胞 HyperCLDB: cl652 HyperCLDB: cl652 CCD-1095Sk CCD-1095Sk CCD-1095Sk cell CCD-1095Sk 细胞 ATCC: CRL-2122 ATCC: CRL-2122 CCRF S-180 II CCRF S-180 II CCRF S-180 II cell CCRF S-180 II 细胞 ATCC: CCL-8 ATCC: CCL-8 HyperCLDB: cl672 HyperCLDB: cl672 LCL-1012 LCL-1012 CCRF-CEM CCRF-CEM CCRF-CEM cell CCRF-CEM 细胞 ATCC: CCL-119 ATCC: CCL-119 CHEMBL: CHEMBL3307641 CHEMBL: CHEMBL3307641 CVCL: CVCL_0207 CVCL: CVCL_0207 EFO: EFO_0002128 EFO: EFO_0002128 LCL-1034 LCL-1034 CEM/C1 CEM/C1 CEM/C1 cell CEM/C1 细胞 ATCC: CRL-2265 ATCC: CRL-2265 CF-1 MEF CF-1 MEF disease: feeder layer 疾病：饲养层 CF-1 MEF cell CF-1 MEF 细胞 ATCC: SCRC-1040 ATCC: SCRC-1040 Cf2Th Cf2Th Cf2Th cell Cf2Th 细胞 ATCC: CRL-1430 ATCC: CRL-1430 LCL-1748 LCL-1748 CFPAC-1 CFPAC-1 disease: ductal adenocarcinoma; cystic fibrosis 疾病：导管腺癌; 囊性纤维化 CFPAC-1 cell CFPAC-1 细胞 ATCC: CRL-1918 ATCC: CRL-1918 CHEMBL: CHEMBL3308032 CHEMBL: CHEMBL3308032 CVCL: CVCL_1119 CVCL: CVCL_1119 Chang liver Chang liver Chang liver cell Chang liver 细胞 HyperCLDB: cl715 HyperCLDB: cl715 CHL CHL CHL cell CHL 细胞 HyperCLDB: cl720 HyperCLDB: cl720 CHO CHO Chinese Hamster Ovary cell 中国仓鼠卵巢细胞 CHO cell CHO 细胞 HyperCLDB: cl721 HyperCLDB: cl721 MeSH: D016466 MeSH: D016466 CHO K1 CHO K1 CHO K1 cell CHO K1 细胞 HyperCLDB: cl731 HyperCLDB: cl731 CHO/dhFr– CHO/dhFr– CHO/dhFr– cell CHO/dhFr– 细胞 ATCC: CRL-9096 ATCC: CRL-9096 HyperCLDB: cl728 HyperCLDB: cl728 CHO-DHFR - CHO-DHFR - CHO-DHFR - cell CHO-DHFR - 细胞 HyperCLDB: cl729 HyperCLDB: cl729 CHO-K1 CHO-K1 CHO-K1 cell CHO-K1 细胞 ATCC: CCL-61 ATCC: CCL-61 CHO-K1 CHO-K1 CHO-K1 cell CHO-K1 细胞 ATCC: CRL-9618 ATCC: CRL-9618 Cl. Ly1+2–/9 Cl. Ly1+2–/9 Cl. Ly1+2–/9 cell Cl. Ly1+2–/9 细胞 ATCC: CRL-8179 ATCC: CRL-8179 Clone 81 Clone 81 Clone 81 cell Clone 81 细胞 HyperCLDB: cl797 HyperCLDB: cl797 Clone C6/36 Clone C6/36 Clone C6/36 cell Clone C6/36 细胞 HyperCLDB: cl799 HyperCLDB: cl799 LCL-1172 LCL-1172 COLO 201 COLO 201 disease: colorectal adenocarcinoma (colon primary) 疾病：结肠直肠腺癌（结肠原发性） COLO 201 cell COLO 201 细胞 ATCC: CCL-224 ATCC: CCL-224 ECACC: 87091201 ECACC: 87091201 LCL-1173 LCL-1173 COLO 205 COLO 205 disease: colorectal adenocarcinoma (colon primary) 疾病：结肠直肠腺癌（结肠原发性） COLO 205 cell COLO 205 细胞 ATCC: CCL-222 ATCC: CCL-222 CHEMBL: CHEMBL3308348 CHEMBL: CHEMBL3308348 CVCL: CVCL_0218 CVCL: CVCL_0218 ECACC: 87061208 ECACC: 87061208 EFO: EFO_0003082 EFO: EFO_0003082 LCL-1197 LCL-1197 COLO 320DM COLO 320DM disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 COLO 320DM cell COLO 320DM 细胞 ATCC: CCL-220 ATCC: CCL-220 CHEMBL: CHEMBL3307647 CHEMBL: CHEMBL3307647 CVCL: CVCL_0219 CVCL: CVCL_0219 EFO: EFO_0002136 EFO: EFO_0002136 COS-1 COS-1 COS-1 cell COS-1 细胞 ATCC: CRL-1650 ATCC: CRL-1650 MeSH: D019556 MeSH: D019556 COS-7 COS-7 COS-7 cell COS-7 细胞 ATCC: CRL-1651 ATCC: CRL-1651 MeSH: D019556 MeSH: D019556 CRFK CRFK CRFK cell CRFK 细胞 ATCC: CCL-94 ATCC: CCL-94 CT26.WT CT26.WT disease: carcinoma; model for testing immunotherapy protocols in vivo 疾病：癌症；体内免疫治疗方案测试模型 CT26.WT cell CT26.WT 细胞 ATCC: CRL-2638 ATCC: CRL-2638 CTLA4 Ig-24 CTLA4 Ig-24 disease: expresses CTLA4Ig fusion protein 疾病：表达CTLA4Ig融合蛋白 CTLA4 Ig-24 cell CTLA4 Ig-24 细胞 ATCC: CRL-10762 ATCC: CRL-10762 CTLL-2 CTLL-2 disease: cytotoxic 疾病：细胞毒性 CTLL-2 cell CTLL-2 细胞 ATCC: TIB-214 ATCC: TIB-214 CV-1 CV-1 CV-1 cell CV-1 细胞 ATCC: CCL-70 ATCC: CCL-70 HyperCLDB: cl929 HyperCLDB: cl929 LCL-1577 LCL-1577 ZN ZN D283 Med D283 Med disease: medulloblastoma (cerebellum primary) 疾病：髓母细胞瘤（小脑原发性） D283 Med cell D283 Med 细胞 ATCC: HTB-185 ATCC: HTB-185 D341 Med D341 Med disease: medulloblastoma 疾病：髓母细胞瘤 D341 Med cell D341 Med 细胞 ATCC: HTB-187 ATCC: HTB-187 LCL-1576 LCL-1576 ZN ZN Daoy Daoy disease: desmoplastic cerebellar medulloblastoma 疾病：结缔组织增生性小脑髓母细胞瘤 Daoy cell Daoy 细胞 ATCC: HTB-186 ATCC: HTB-186 CHEMBL: CHEMBL3308510 CHEMBL: CHEMBL3308510 CVCL: CVCL_1167 CVCL: CVCL_1167 LCL-2018 LCL-2018 Daudi Daudi Daudi cell Daudi 细胞 ATCC: CCL-213 ATCC: CCL-213 CHEMBL: CHEMBL3307579 CHEMBL: CHEMBL3307579 CVCL: CVCL_0008 CVCL: CVCL_0008 EFO: EFO_0002169 EFO: EFO_0002169 HyperCLDB: cl995 HyperCLDB: cl995 DH82 DH82 disease: malignant 疾病：恶性 DH82 cell DH82 细胞 ATCC: CRL-10389 ATCC: CRL-10389 LCL-1175 LCL-1175 DLD-1 DLD-1 disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 DLD-1 cell DLD-1 细胞 ATCC: CCL-221 ATCC: CCL-221 CHEMBL: CHEMBL3307580 CHEMBL: CHEMBL3307580 CVCL: CVCL_0248 CVCL: CVCL_0248 LCL-1831 LCL-1831 DMS 153 DMS 153 disease: carcinoma; small cell lung cancer (lung primary) 疾病：癌; 小细胞肺癌（肺原发性） DMS 153 cell DMS 153 细胞 ATCC: CRL-2064 ATCC: CRL-2064 DT40 DT40 DT40 cell DT40 细胞 ATCC: CRL-2111 ATCC: CRL-2111 LCL-1146 LCL-1146 DU 145 DU 145 disease: carcinoma (prostate primary) 疾病：癌（前列腺原发性） DU 145 cell DU 145 细胞 ATCC: HTB-81 ATCC: HTB-81 LCL-1323 LCL-1323 DU4475 DU4475 disease: ductal carcinoma 疾病：导管癌 DU4475 cell DU4475 细胞 ATCC: HTB-123 ATCC: HTB-123 HyperCLDB: cl5264 HyperCLDB: cl5264 DXB-11 DXB-11 DXB-11 cell DXB-11 细胞 HyperCLDB: cl1114 HyperCLDB: cl1114 E.G7-OVA E.G7-OVA E.G7-OVA cell E.G7-OVA 细胞 ATCC: CRL-2113 ATCC: CRL-2113 LCL-2032 LCL-2032 EB-3 EB-3 disease: Burkitts lymphoma 疾病：伯基特淋巴瘤 EB-3 cell EB-3 细胞 ATCC: CCL-85 ATCC: CCL-85 CHEMBL: CHEMBL3308824 CHEMBL: CHEMBL3308824 CVCL: CVCL_1185 CVCL: CVCL_1185 EBTr (NBL-4) EBTr (NBL-4) EBTr (NBL-4) cell EBTr (NBL-4) 细胞 ATCC: CCL-44 ATCC: CCL-44 ECV304 ECV304 ECV304 cell ECV304 细胞 HyperCLDB: cl5185 HyperCLDB: cl5185 ECV-304 ECV-304 disease: carcinoma, urinary 疾病：癌，尿 ECV-304 cell ECV-304 细胞 HyperCLDB: cl5266 HyperCLDB: cl5266 EL4 EL4 WEB: http://web.expasy.org/cellosaurus/CVCL_0255 WEB: http://web.expasy.org/cellosaurus/CVCL_0255 immortal T cell line cell that is derived from blood T cells from mouse C57BL/6 inbred strain that has disease mouse lymphoma. 患有疾病小鼠淋巴瘤的小鼠C57BL/6近交系的血液T细胞衍生的永生T细胞系细胞。 EL4 cell EL4 细胞 ATCC: TIB-39 ATCC: TIB-39 RRID:CVCL_0255 RRID:CVCL_0255 EL4.IL-2 EL4.IL-2 EL4.IL-2 cell EL4.IL-2 细胞 ATCC: TIB-181 ATCC: TIB-181 LCL-1806 LCL-1806 ES-2 ES-2 disease: clear cell carcinoma 疾病：透明细胞癌 ES-2 cell ES-2 细胞 ATCC: CRL-1978 ATCC: CRL-1978 ES-D3 ES-D3 disease: pluripotent 疾病：多能 ES-D3 cell ES-D3 细胞 ATCC: CRL-11632 ATCC: CRL-11632 ES-D3 ES-D3 ES-D3 cell ES-D3 细胞 ATCC: CRL-1934 ATCC: CRL-1934 F11 F11 F11 cell F11 细胞 HyperCLDB: cl1188 HyperCLDB: cl1188 F4/80 F4/80 disease: hybridoma 疾病：杂交瘤 F4/80 cell F4/80 细胞 ATCC: HB-198 ATCC: HB-198 F9 F9 disease: embryonic carcinoma; testicular teratoma 疾病：胚胎癌; 睾丸畸胎瘤 F9 cell F9 细胞 ATCC: CRL-1720 ATCC: CRL-1720 LCL-1201 LCL-1201 FaDu FaDu disease: squamous cell carcinoma 疾病：鳞状细胞癌 FaDu cell FaDu 细胞 ATCC: HTB-43 ATCC: HTB-43 CHEMBL: CHEMBL3307943 CHEMBL: CHEMBL3307943 CVCL: CVCL_1218 CVCL: CVCL_1218 EFO: EFO_0002178 EFO: EFO_0002178 LCL-1131 LCL-1131 Farage Farage disease: non-Hodgkins lymphoma 疾病：非霍奇金淋巴瘤 Farage cell Farage 细胞 ATCC: CRL-2630 ATCC: CRL-2630 FDCP-1 FDCP-1 FDCP-1 cell FDCP-1 细胞 HyperCLDB: cl5275 HyperCLDB: cl5275 FDC-P1 FDC-P1 FDC-P1 cell FDC-P1 细胞 ATCC: CRL-12103 ATCC: CRL-12103 FER FER FER cell FER 细胞 HyperCLDB: cl1223 HyperCLDB: cl1223 FO FO disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 FO cell FO 细胞 ATCC: CRL-1646 ATCC: CRL-1646 FOX-NY FOX-NY disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 FOX-NY cell FOX-NY 细胞 ATCC: CRL-1732 ATCC: CRL-1732 FRhK-4 FRhK-4 FRhK-4 cell FRhK-4 细胞 ATCC: CRL-1688 ATCC: CRL-1688 LCL-1511 LCL-1511 G-401 G-401 disease: rhabdoid tumor 疾病：横纹肌样瘤 G-401 cell G-401 细胞 ATCC: CRL-1441 ATCC: CRL-1441 CHEMBL: CHEMBL3308394 CHEMBL: CHEMBL3308394 CVCL: CVCL_0270 CVCL: CVCL_0270 EFO: EFO_0002179 EFO: EFO_0002179 GH3 GH3 GH3 cell GH3 细胞 HyperCLDB: cl1456 HyperCLDB: cl1456 GK1.5 GK1.5 disease: hybridoma 疾病：杂交瘤 GK1.5 cell GK1.5 细胞 ATCC: TIB-207 ATCC: TIB-207 HyperCLDB: cl5190 HyperCLDB: cl5190 GLC-82 GLC-82 GLC-82 cell GLC-82 细胞 HyperCLDB: cl4909 HyperCLDB: cl4909 LCL-1406 LCL-1406 GOS-3 GOS-3 GOS-3 cell GOS-3 细胞 DSMZ: ACC 408 DSMZ: ACC 408 HyperCLDB: cl5278 HyperCLDB: cl5278 GR-M GR-M GR-M cell GR-M 细胞 HyperCLDB: cl1513 HyperCLDB: cl1513 H1HeLa H1HeLa H1HeLa cell H1HeLa 细胞 ATCC: CRL-1958 ATCC: CRL-1958 LCL-1401 LCL-1401 H4 H4 disease: neuroglioma 疾病：神经胶质瘤 H4 cell H4 细胞 ATCC: HTB-148 ATCC: HTB-148 CHEMBL: CHEMBL3308507 CHEMBL: CHEMBL3308507 CVCL: CVCL_1239 CVCL: CVCL_1239 EFO: EFO_0002184 EFO: EFO_0002184 H460 H460 H460 cell H460 细胞 HyperCLDB: cl1548 HyperCLDB: cl1548 H-4-II-E H-4-II-E disease: hepatoma 疾病：肝癌 H-4-II-E cell H-4-II-E 细胞 ATCC: CRL-1548 ATCC: CRL-1548 H4-II-E-C3 H4-II-E-C3 disease: hepatoma 疾病：肝癌 H4-II-E-C3 cell H4-II-E-C3 细胞 ATCC: CRL-1600 ATCC: CRL-1600 LCL-1144 LCL-1144 H9 H9 H9 cell H9 细胞 ATCC: HTB-176 ATCC: HTB-176 CHEMBL: CHEMBL3308402 CHEMBL: CHEMBL3308402 CVCL: CVCL_1240 CVCL: CVCL_1240 EFO: EFO_0003045 EFO: EFO_0003045 Sanger: COSMIC ID:907043 Sanger: COSMIC ID:907043 H9c2(2-1) H9c2(2-1) H9c2(2-1) cell H9c2(2-1) 细胞 ATCC: CRL-1446 ATCC: CRL-1446 HyperCLDB: cl1558 HyperCLDB: cl1558 HBL-100 HBL-100 HBL-100 cell HBL-100 细胞 HyperCLDB: cl1571 HyperCLDB: cl1571 LCL-1331 LCL-1331 HCC1937 HCC1937 disease: primary ductal carcinoma 疾病：原发性导管癌 HCC1937 cell HCC1937 细胞 ATCC: CRL-2336 ATCC: CRL-2336 CHEMBL: CHEMBL3308371 CHEMBL: CHEMBL3308371 CVCL: CVCL_0290 CVCL: CVCL_0290 EFO: EFO_0001174 EFO: EFO_0001174 LCL-1334 LCL-1334 HCC38 HCC38 disease: primary ductal carcinoma 疾病：原发性导管癌 HCC38 cell HCC38 细胞 ATCC: CRL-2314 ATCC: CRL-2314 CHEMBL: CHEMBL3308578 CHEMBL: CHEMBL3308578 CVCL: CVCL_1267 CVCL: CVCL_1267 EFO: EFO_0001180 EFO: EFO_0001180 HCC827 HCC827 HCC827 cell HCC827 细胞 ATCC: CRL-2868 ATCC: CRL-2868 LCL-1161 LCL-1161 HCT 116 HCT 116 HCT-116 HCT-116 WEB: http://web.expasy.org/cellosaurus/CVCL_0291 WEB: http://web.expasy.org/cellosaurus/CVCL_0291 WEB: https://www.atcc.org/products/all/CCL-247.aspx WEB: https://www.atcc.org/products/all/CCL-247.aspx HCT116 HCT116 immortal human epithelial cell line cell that was derived from some colon epithelial cell from a human patient with the disease colon carcinoma 患有疾病结肠癌的患者的一些结肠上皮细胞衍生的永生人类上皮细胞系细胞 disease: colorectal carcinoma 疾病：结直肠癌 HCT 116 cell HCT 116 细胞 ATCC: CCL-247 ATCC: CCL-247 ECACC: 91091005 ECACC: 91091005 HyperCLDB: cl1575 HyperCLDB: cl1575 MeSH: D045325 MeSH: D045325 RRID:CVCL_0291 RRID:CVCL_0291 LCL-1178 LCL-1178 HCT-15 HCT-15 disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 HCT-15 cell HCT-15 细胞 ATCC: CCL-225 ATCC: CCL-225 CHEMBL: CHEMBL3307945 CHEMBL: CHEMBL3307945 CVCL: CVCL_0292 CVCL: CVCL_0292 EFO: EFO_0002188 EFO: EFO_0002188 LCL-1198 LCL-1198 HCT-8 HCT-8 HCT-8 cell HCT-8 细胞 CHEMBL: CHEMBL3308035 CHEMBL: CHEMBL3308035 CVCL: CVCL_2478 CVCL: CVCL_2478 ECACC: 90032006 ECACC: 90032006 EFO: EFO_0002189 EFO: EFO_0002189 HyperCLDB: cl4949 HyperCLDB: cl4949 HEC-1-A HEC-1-A HEC-1-A cell HEC-1-A 细胞 ATCC: HTB-112 ATCC: HTB-112 HEC-1-B HEC-1-B HEC-1-B cell HEC-1-B 细胞 ATCC: HTB-113 ATCC: HTB-113 LCL-1053 LCL-1053 HEL HEL HEL cell HEL 细胞 CHEMBL: CHEMBL3308090 CHEMBL: CHEMBL3308090 CVCL: CVCL_0001 CVCL: CVCL_0001 DSMZ: ACC 11 DSMZ: ACC 11 HyperCLDB: cl1585 HyperCLDB: cl1585 LCL-1512 LCL-1512 HeLa HeLa WEB: http://en.wikipedia.org/wiki/HeLa WEB: http://en.wikipedia.org/wiki/HeLa WEB: http://www.atcc.org/products/all/CCL-2.aspx WEB: http://www.atcc.org/products/all/CCL-2.aspx WEB: https://www.ncbi.nlm.nih.gov/pubmed/12001993 WEB: https://www.ncbi.nlm.nih.gov/pubmed/12001993 1951年2月8日从一位于1951年10月4日因癌症死亡的病人Henrietta Lacks中获取的宫颈腺癌癌细胞衍生的永生人类子宫颈衍生上皮细胞系。 an immortal human uterine cervix-derived epithelial cell line that was derived from cervical adenocarcinoma cancer cells taken on February 8, 1951 from Henrietta Lacks, a patient who died of her cancer on October 4, 1951. HeLa cell HeLa 细胞 ATCC: CCL-2 ATCC: CCL-2 CHEMBL: CHEMBL3308376 CHEMBL: CHEMBL3308376 EFO: EFO_0001185 EFO: EFO_0001185 HyperCLDB: cl1601 HyperCLDB: cl1601 MeSH: D006367 MeSH: D006367 RRID:CVCL_0030 RRID:CVCL_0030 HeLa 229 HeLa 229 HeLa 229 cell HeLa 229 细胞 ATCC: CCL-2.1 ATCC: CCL-2.1 HeLa S3 HeLa S3 HeLa S3 cell HeLa S3 细胞 ATCC: CCL-2.2 ATCC: CCL-2.2 HeLa229 HeLa229 disease: carcinoma, epitheloid 疾病：癌，上皮样 HeLa229 cell HeLa229 细胞 HyperCLDB: cl1606 HyperCLDB: cl1606 Hep 3B Hep 3B disease: carcinoma, hepatocellular 疾病：癌，肝细胞癌 Hep 3B cell Hep 3B 细胞 HyperCLDB: cl1627 HyperCLDB: cl1627 LCL-1924 LCL-1924 Hep 3B2.1-7 Hep 3B2.1-7 Hep 3B2.1-7 cell Hep 3B2.1-7 细胞 ATCC: HB-8064 ATCC: HB-8064 LCL-1925 LCL-1925 HEP G2 HEP G2 HEP-G2 HEP-G2 HEPG2 HEPG2 Hep G2 Hep G2 HepG2 HepG2 WEB: http://web.expasy.org/cellosaurus/CVCL_0027 WEB: http://web.expasy.org/cellosaurus/CVCL_0027 WEB: https://en.wikipedia.org/wiki/Hep_G2 WEB: https://en.wikipedia.org/wiki/Hep_G2 WEB: https://www.atcc.org/products/all/HB-8065.aspx WEB: https://www.atcc.org/products/all/HB-8065.aspx Hep G2 cell Hep G2 细胞 ATCC: HB-8065 ATCC: HB-8065 HyperCLDB: cl1644 HyperCLDB: cl1644 RRID:CVCL_0027 Hep-2 Hep-2 disease: carcinoma, squamous cell 疾病：癌，鳞状细胞 Hep-2 cell Hep-2 细胞 HyperCLDB: cl1647 HyperCLDB: cl1647 HyperCLDB: cl1650 HyperCLDB: cl1650 HyperCLDB: cl1655 HyperCLDB: cl1655 HEP-3B HEP-3B disease: carcinoma, hepatocellular 疾病：癌，肝细胞癌 HEP-3B cell HEP-3B 细胞 HyperCLDB: cl1629 HyperCLDB: cl1629 Hepa 1-6 Hepa 1-6 disease: hepatoma 疾病：肝癌 Hepa 1-6 cell Hepa 1-6 细胞 ATCC: CRL-1830 ATCC: CRL-1830 HyperCLDB: cl1659 HyperCLDB: cl1659 HFL HFL HFL cell HFL 细胞 HyperCLDB: cl5200 HyperCLDB: cl5200 HFL1 HFL1 HFL1 cell HFL1 细胞 ATCC: CCL-153 ATCC: CCL-153 hFOB 1.19 hFOB 1.19 disease: SV40 large T antigen transfected 疾病：SV40大T抗原转染 hFOB 1.19 cell hFOB 1.19 细胞 ATCC: CRL-11372 ATCC: CRL-11372 LCL-1882 LCL-1882 HGC-27 HGC-27 HGC-27 cell HGC-27 细胞 CHEMBL: CHEMBL3308741 CHEMBL: CHEMBL3308741 CVCL: CVCL_1279 CVCL: CVCL_1279 ECACC: 94042256 ECACC: 94042256 HyperCLDB: cl1673 HyperCLDB: cl1673 HK-2 HK-2 disease: HPV-16 transformed 疾病：HPV-16转化 HK-2 cell HK-2 细胞 ATCC: CRL-2190 ATCC: CRL-2190 LCL-1054 LCL-1054 HL-60 HL-60 disease: promyeloblast; acute promyelocytic leukemia 疾病：早幼粒细胞; 急性早幼粒细胞白血病 HL-60 cell HL-60 细胞 ATCC: CCL-240 ATCC: CCL-240 CHEMBL: CHEMBL3307654 CHEMBL: CHEMBL3307654 CVCL: CVCL_0002 CVCL: CVCL_0002 EFO: EFO_0002793 EFO: EFO_0002793 HyperCLDB: cl1684 HyperCLDB: cl1684 HyperCLDB: cl1689 HyperCLDB: cl1689 MeSH: D018922 MeSH: D018922 HLF-a HLF-a disease: epidermoid carcinoma 疾病：表皮样癌 HLF-a cell HLF-a 细胞 ATCC: CCL-199 ATCC: CCL-199 HMy2.CIR HMy2.CIR WEB: http://web.expasy.org/cellosaurus/CVCL_3714 WEB: http://web.expasy.org/cellosaurus/CVCL_3714 immortal human B cell line cell that derives from some B cell from a human blood sample 来源于人血液样品B细胞的永生人B细胞系细胞 HMy2.CIR cell HMy2.CIR 细胞 ATCC: CRL-1993 ATCC: CRL-1993 RRID: CVCL_3714 RRID: CVCL_3714 LCL-1421 LCL-1421 ZN ZN HOS HOS HOS cell HOS 细胞 ATCC: CRL-1543 ATCC: CRL-1543 CHEMBL: CHEMBL3308811 CHEMBL: CHEMBL3308811 CVCL: CVCL_0312 CVCL: CVCL_0312 ECACC: 87070202 ECACC: 87070202 EFO: EFO_0002196 EFO: EFO_0002196 HOS (TE85, Clone F5) HOS (TE85, Clone F5) HOS (TE85, Clone F5) cell HOS (TE85, Clone F5) 细胞 HyperCLDB: cl1703 HyperCLDB: cl1703 LCL-1735 LCL-1735 HPAC HPAC HPAC cell HPAC 细胞 ATCC: CRL-2119 ATCC: CRL-2119 CHEMBL: CHEMBL3308388 CHEMBL: CHEMBL3308388 CVCL: CVCL_3517 CVCL: CVCL_3517 EFO: EFO_0002197 EFO: EFO_0002197 Hs 578Bst Hs 578Bst Hs 578Bst cell Hs 578Bst 细胞 ATCC: HTB-125 ATCC: HTB-125 LCL-1315 LCL-1315 Hs 578T Hs 578T HS578T HS578T disease: ductal carcinoma 疾病：导管癌 Hs 578T cell Hs 578T 细胞 ATCC: HTB-126 ATCC: HTB-126 LCL-1402 LCL-1402 Hs 683 Hs 683 Hs 683 cell Hs 683 细胞 ATCC: HTB-138 ATCC: HTB-138 CHEMBL: CHEMBL3308452 CHEMBL: CHEMBL3308452 CVCL: CVCL_0844 CVCL: CVCL_0844 LCL-1883 LCL-1883 Hs 746T Hs 746T disease: gastric carcinoma (stomach primary 疾病：胃癌（胃原发） Hs 746T cell Hs 746T 细胞 ATCC: HTB-135 ATCC: HTB-135 CHEMBL: CHEMBL3307498 CHEMBL: CHEMBL3307498 CVCL: CVCL_0333 CVCL: CVCL_0333 LCL-1737 LCL-1737 Hs 766T Hs 766T disease: carcinoma (pancreas primary) 疾病：癌（胰腺原发性） Hs 766T cell Hs 766T 细胞 ATCC: HTB-134 ATCC: HTB-134 Hs68 Hs68 disease: aspartoacylase deficiency; possible Canavan disease 疾病：天冬酰胺酶缺乏症;可能是卡纳万病 Hs68 cell Hs68 细胞 ATCC: CRL-1635 ATCC: CRL-1635 HSF HSF HSF cell HSF 细胞 HyperCLDB: cl1731 HyperCLDB: cl1731 LCL-1435 LCL-1435 HT 1080 HT 1080 HT 1080 cell HT 1080 细胞 HyperCLDB: cl1736 HyperCLDB: cl1736 HT-1080 HT-1080 disease: fibrosarcoma 疾病：纤维肉瘤 HT-1080 cell HT-1080 细胞 ATCC: CCL-121 ATCC: CCL-121 LCL-1180 LCL-1180 HT-29 HT-29 HT29 HT29 disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 HT-29 cell HT-29 细胞 ATCC: HTB-38 ATCC: HTB-38 CHEMBL: CHEMBL3307768 CHEMBL: CHEMBL3307768 CVCL: CVCL_0320 CVCL: CVCL_0320 EFO: EFO_0001193 EFO: EFO_0001193 HyperCLDB: cl1752 HyperCLDB: cl1752 MeSH: D019073 MeSH: D019073 HuT 102 HuT 102 disease: lymphoma; mycosis fungoides 疾病：淋巴瘤; 蕈样霉菌病 HuT 102 cell HuT 102 细胞 ATCC: TIB-162 ATCC: TIB-162 LCL-2005 LCL-2005 HuT 78 HuT 78 HuT 78 cell HuT 78 细胞 ATCC: TIB-161 ATCC: TIB-161 CHEMBL: CHEMBL3307500 CHEMBL: CHEMBL3307500 CVCL: CVCL_0337 CVCL: CVCL_0337 EFO: EFO_0002209 EFO: EFO_0002209 HyperCLDB: cl1779 HyperCLDB: cl1779 HUV-EC-C HUV-EC-C HUVEC HUVEC WEB: https://www.atcc.org/Products/All/CRL-1730.aspx WEB: https://www.atcc.org/Products/All/CRL-1730.aspx HUV-EC-C cell HUV-EC-C 细胞 ATCC: CRL-1730 ATCC: CRL-1730 LCL-1027 LCL-1027 I 2.1 I 2.1 I 2.1 cell I 2.1 细胞 ATCC: CRL-2572 ATCC: CRL-2572 IB-RS-2 IB-RS-2 IB-RS-2 cell IB-RS-2 细胞 HyperCLDB: cl1794 HyperCLDB: cl1794 IEC-6 IEC-6 disease: epithelium 疾病：上皮 IEC-6 cell IEC-6 细胞 ATCC: CRL-1592 ATCC: CRL-1592 HyperCLDB: cl1802 HyperCLDB: cl1802 LCL-1958 LCL-1958 IM-9 IM-9 IM-9 cell IM-9 细胞 ATCC: CCL-159 ATCC: CCL-159 LCL-1965 LCL-1965 IMR-32 IMR-32 IMR-32 cell IMR-32 细胞 ATCC: CCL-127 ATCC: CCL-127 CHEMBL: CHEMBL3307656 CHEMBL: CHEMBL3307656 CVCL: CVCL_0346 CVCL: CVCL_0346 EFO: EFO_0001195 EFO: EFO_0001195 HyperCLDB: cl2880 HyperCLDB: cl2880 IMR-90 IMR-90 IMR9 IMR9 IMR90 IMR90 WEB: https://www.atcc.org/products/all/CCL-186.aspx WEB: https://www.atcc.org/products/all/CCL-186.aspx IMR-90 cell IMR-90 细胞 ATCC: CCL-186 ATCC: CCL-186 J 111 J 111 J 111 cell J 111 细胞 HyperCLDB: cl2915 HyperCLDB: cl2915 J774A.1 J774A.1 J774A.1 cell J774A.1 细胞 ATCC: TIB-67 ATCC: TIB-67 HyperCLDB: cl2928 HyperCLDB: cl2928 LCL-1714 LCL-1714 J82 J82 disease: transitional cell carcinoma 疾病：移行细胞癌 J82 cell J82 细胞 ATCC: HTB-1 ATCC: HTB-1 CHEMBL: CHEMBL3308508 CHEMBL: CHEMBL3308508 CVCL: CVCL_0359 CVCL: CVCL_0359 EFO: EFO_0002210 EFO: EFO_0002210 LCL-1491 LCL-1491 JAR JAR JAR cell JAR 细胞 ATCC: HTB-144 ATCC: HTB-144 CHEMBL: CHEMBL3307657 CHEMBL: CHEMBL3307657 CVCL: CVCL_0360 CVCL: CVCL_0360 EFO: EFO_0002211 EFO: EFO_0002211 LCL-1492 LCL-1492 JEG-3 JEG-3 JEG-3 cell JEG-3 细胞 CHEMBL: CHEMBL3308202 CHEMBL: CHEMBL3308202 CVCL: CVCL_0363 CVCL: CVCL_0363 HyperCLDB: cl2936 HyperCLDB: cl2936 Sanger: COSMIC ID:907176 Sanger: COSMIC ID:907176 JIII JIII disease: leukemia, monocytic 疾病：白血病，单核细胞 JIII cell JIII 细胞 HyperCLDB: cl2916 HyperCLDB: cl2916 JM JM JM cell JM 细胞 HyperCLDB: cl2948 HyperCLDB: cl2948 JURKAT JURKAT WEB: https://en.wikipedia.org/wiki/Jurkat_cells WEB: https://en.wikipedia.org/wiki/Jurkat_cells an immortalized human T lymphocyte cell that was derived in the late 1970s from the peripheral blood of a 14-year-old boy with T cell leukemia 永生的人类T淋巴细胞，衍生自20世纪70年代后期一名14岁的患有T细胞白血病的男孩的外周血 disease: leukemia, T cell 疾病：白血病，T细胞 JURKAT cell JURKAT 细胞 HyperCLDB: cl5296 HyperCLDB: cl5296 MeSH: D019169 MeSH: D019169 RRID: CVCL_0065 RRID: CVCL_0065 LCL-1030 LCL-1030 JX JX Jurkat, Clone E6-1 Jurkat, Clone E6-1 Jurkat, Clone E6-1 cell Jurkat, Clone E6-1 细胞 ATCC: TIB-152 ATCC: TIB-152 HyperCLDB: cl2962 HyperCLDB: cl2962 LCL-1099 LCL-1099 JVM-2 JVM-2 JVM-2 cell JVM-2 细胞 CHEMBL: CHEMBL3308530 CHEMBL: CHEMBL3308530 COSMIC ID:907269 COSMIC ID:907269 CVCL: CVCL_1319 CVCL: CVCL_1319 HyperCLDB: cl2965 HyperCLDB: cl2965 LCL-1103 LCL-1103 K-562 K-562 disease: chronic myelo- genous leukemia (bone marrow primary) 疾病：慢性骨髓性白血病（骨髓原发性） K-562 cell K-562 细胞 ATCC: CCL-243 ATCC: CCL-243 CHEMBL: CHEMBL3308378 CHEMBL: CHEMBL3308378 CVCL: CVCL_0004 CVCL: CVCL_0004 EFO: EFO_0002067 EFO: EFO_0002067 HyperCLDB: cl2980 HyperCLDB: cl2980 MeSH: D020014 MeSH: D020014 LCL-1055 LCL-1055 Kasumi-1 Kasumi-1 disease: acute myeloblastic leukemia 疾病：急性成髓细胞性白血病 Kasumi-1 cell Kasumi-1 细胞 ATCC: CRL-2724 ATCC: CRL-2724 LCL-2003 LCL-2003 KATO III KATO III disease: gastric carcinoma (stomach primary) 疾病：胃癌（胃原发性） KATO III cell KATO III 细胞 ATCC: HTB-103 ATCC: HTB-103 HyperCLDB: cl3000 HyperCLDB: cl3000 HyperCLDB: cl5214 HyperCLDB: cl5214 KB KB KB cell KB 细胞 ATCC: CCL-17 ATCC: CCL-17 MeSH: D007624 MeSH: D007624 KC-4G3 KC-4G3 disease: hybridoma 疾病：杂交瘤 KC-4G3 cell KC-4G3 细胞 ATCC: HB-8709 ATCC: HB-8709 LCL-1056 LCL-1056 KG-1 KG-1 KG-1 cell KG-1 细胞 LCL-1057 LCL-1057 KG-1a KG-1a disease: promyeloblast; acute lympho- blastic leukemia 疾病：早幼粒细胞; 急性淋巴细胞白血病 KG-1a cell KG-1a 细胞 ATCC: CCL-246.1 ATCC: CCL-246.1 HyperCLDB: cl3027 HyperCLDB: cl3027 LCL-1505 LCL-1505 ZN ZN KLE KLE KLE cell KLE 细胞 ATCC: CRL-1622 ATCC: CRL-1622 CHEMBL: CHEMBL3308518 CHEMBL: CHEMBL3308518 CVCL: CVCL_1329 CVCL: CVCL_1329 EFO: EFO_0002220 EFO: EFO_0002220 Sanger: COSMIC ID:924187 Sanger: COSMIC ID:924187 LCL-1106 LCL-1106 KU812 KU812 disease: chronic myelogenous leukemia 慢性粒细胞白血病 KU812 cell KU812 细胞 ATCC: CRL-2099 ATCC: CRL-2099 LCL-1107 LCL-1107 KU812E KU812E disease: chronic myelogenous leukemia 慢性粒细胞白血病 KU812E cell KU812E 细胞 ATCC: CRL-2100 ATCC: CRL-2100 HyperCLDB: cl3071 HyperCLDB: cl3071 L Cells L Cells L Cells cell L Cells 细胞 ATCC: CRL-2648 ATCC: CRL-2648 HyperCLDB: cl3075 HyperCLDB: cl3075 MeSH: D007739 MeSH: D007739 L 929 L 929 L 929 cell L 929 细胞 HyperCLDB: cl3154 HyperCLDB: cl3154 L Wnt-3A L Wnt-3A L Wnt-3A cell L Wnt-3A 细胞 ATCC: CRL-2647 ATCC: CRL-2647 L1210 L1210 L1210 cell L1210 细胞 ATCC: CCL-219 ATCC: CCL-219 L5178Y TK+/– (clone 3.7.2C) L5178Y TK+/– (clone 3.7.2C) L5178Y TK+/– (clone 3.7.2C) cell L5178Y TK+/– (clone 3.7.2C) 细胞 ATCC: CRL-9518 ATCC: CRL-9518 L6 L6 L6 cell L6 细胞 ATCC: CRL-1458 ATCC: CRL-1458 L929 L929 L929 cell L929 细胞 HyperCLDB: cl3159 HyperCLDB: cl3159 L-929 L-929 L-929 cell L-929 细胞 HyperCLDB: cl3158 HyperCLDB: cl3158 L929(NCTC) L929(NCTC) L929(NCTC) cell L929(NCTC) 细胞 HyperCLDB: cl3160 HyperCLDB: cl3160 Lec1 Lec1 disease: lacks GlcNAc glycosyl transferase function 疾病：缺乏GlcNAc糖基转移酶功能 Lec1 cell Lec1 细胞 ATCC: CRL-1735 ATCC: CRL-1735 LLC-MK subscript(2) LLC-MK subscript(2) LLC-MK subscript(2) cell LLC-MK subscript(2) 细胞 ATCC: CCL-7, CCL-7.1 ATCC: CCL-7, CCL-7.1 HyperCLDB: cl3214 HyperCLDB: cl3214 HyperCLDB: cl3220 HyperCLDB: cl3220 LLC-PK subscript(1) LLC-PK subscript(1) LLC-PK subscript(1) cell LLC-PK subscript(1) 细胞 ATCC: CL-101 ATCC: CL-101 HyperCLDB: cl3221 HyperCLDB: cl3221 MeSH: D018374 MeSH: D018374 LLC-WRC 256 LLC-WRC 256 LLC-WRC 256 cell LLC-WRC 256 细胞 ATCC: CCL-38 ATCC: CCL-38 L-M(TK–) L-M(TK–) L-M(TK–) cell L-M(TK–) 细胞 ATCC: CCL-1.3 ATCC: CCL-1.3 HyperCLDB: cl3097 HyperCLDB: cl3097 LNCAP LNCAP LNCAP cell LNCAP 细胞 HyperCLDB: cl3243 HyperCLDB: cl3243 LNCaP clone FGC LNCaP clone FGC disease: carcinoma (prostate primary) 疾病：癌（前列腺原发性） LNCaP clone FGC cell LNCaP clone FGC 细胞 ATCC: CRL-1740 ATCC: CRL-1740 LNCaP.FGC LNCaP.FGC LNCaP.FGC cell LNCaP.FGC 细胞 HyperCLDB: cl3245 HyperCLDB: cl3245 HyperCLDB: cl4960 HyperCLDB: cl4960 LOVO LOVO LOVO cell LOVO 细胞 HyperCLDB: cl5318 HyperCLDB: cl5318 LS 174T LS 174T disease: colorectal adenocarcinoma 疾病：大肠腺癌 LS 174T cell LS 174T 细胞 ATCC: CL-188 ATCC: CL-188 LS 180 LS 180 disease: colorectal adenocarcinoma 疾病：大肠腺癌 LS 180 cell LS 180 细胞 ATCC: CL-187 ATCC: CL-187 LS132.1D9 LS132.1D9 LS132.1D9 cell LS132.1D9 细胞 ATCC: HB-12549 ATCC: HB-12549 LCL-1182 LCL-1182 LS174T LS174T LS174T cell LS174T 细胞 CHEMBL: CHEMBL3307536 CHEMBL: CHEMBL3307536 CVCL: CVCL_1384 CVCL: CVCL_1384 ECACC: 87060401 ECACC: 87060401 EFO: EFO_0002227 EFO: EFO_0002227 HyperCLDB: cl3261 HyperCLDB: cl3261 LCL-1183 LCL-1183 LS180 LS180 LS180 cell LS180 细胞 ECACC: 87021202 ECACC: 87021202 HyperCLDB: cl3263 HyperCLDB: cl3263 HyperCLDB: cl7120 HyperCLDB: cl7120 LTK- LTK- LTK- cell LTK- 细胞 HyperCLDB: cl3267 HyperCLDB: cl3267 M-07e M-07e disease: leukemia, acute megakaryoblastic 疾病：白血病、急性巨核细胞 M-07e cell M-07e 细胞 HyperCLDB: cl3281 HyperCLDB: cl3281 M-1 M-1 M-1 cell M-1 细胞 ATCC: CRL-2038 ATCC: CRL-2038 M18/2.a.12.7 M18/2.a.12.7 disease: hybridoma 疾病：杂交瘤 M18/2.a.12.7 cell M18/2.a.12.7 细胞 ATCC: TIB-218 ATCC: TIB-218 M2-10B4 M2-10B4 disease: bone marrow, stroma 疾病：骨髓，间质 M2-10B4 cell M2-10B4 细胞 ATCC: CRL-1972 ATCC: CRL-1972 M3/38.1.2.8 HL.2 M3/38.1.2.8 HL.2 disease: hybridoma 疾病：杂交瘤 M3/38.1.2.8 HL.2 cell M3/38.1.2.8 HL.2 细胞 ATCC: TIB-166 ATCC: TIB-166 M5076 M5076 M5076 cell M5076 细胞 HyperCLDB: cl3317 HyperCLDB: cl3317 M-7 M-7 disease: fibrosarcoma 疾病：纤维肉瘤 M-7 cell M-7 细胞 ATCC: CRL-2804 ATCC: CRL-2804 MA 104 MA 104 MA 104 cell MA 104 细胞 HyperCLDB: cl3324 HyperCLDB: cl3324 MA104 MA104 MA104 cell MA104 细胞 HyperCLDB: cl3327 HyperCLDB: cl3327 MARC 145 MARC 145 MARC 145 cell MARC 145 细胞 HyperCLDB: cl5226 HyperCLDB: cl5226 LCL-1538 LCL-1538 ZN ZN MC/CAR MC/CAR disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 MC/CAR cell MC/CAR 细胞 ATCC: CRL-8083 ATCC: CRL-8083 MC3T3-E1 MC3T3-E1 MC3T3-E1 cell MC3T3-E1 细胞 HyperCLDB: cl40 HyperCLDB: cl40 MC3T3-E1 Subclone 14 MC3T3-E1 Subclone 14 MC3T3-E1 Subclone 14 cell MC3T3-E1 Subclone 14 细胞 ATCC: CRL-2594 ATCC: CRL-2594 LCL-2085 LCL-2085 Amelogenin: X; CSF1PO: 10,12; D13S317: 8,9; D16S539: 11,12; D18S51: 18,19; D21S11: 28,30; D3S1358: 14,18; D5S818: 10,13; D7S820: 10,11; D8S1179: 14,16; FGA: 22,24; Penta D: 10,12; Penta E: 13,14; TH01: 8,9.3; TPOX: 9,11; vWA: 15,17 Amelogenin: X; CSF1PO: 10,12; D13S317: 8,9; D16S539: 11,12; D18S51: 18,19; D21S11: 28,30; D3S1358:14,18; D5S818: 10,13; D7S820: 10,11; D8S1179: 14,16; FGA: 22,24; Penta D: 10,12; Penta E: 13,14; TH01: 8,9.3; TPOX: 9,11; vWA: 15,17 MCF-10A is a spontaneously immortalized, but nontransformed human mammary epithelial cell line cell that was derived from the breast tissue of a 36-year-old patient with fibrocystic changes MCF-10A是一种自发性永生化但未转化的人乳腺上皮细胞系细胞，其源自一位36岁患有纤维囊性改变的患者的乳腺组织 MCF 10A MCF 10A MCF-10A MCF-10A MCF.10A MCF.10A MCF10A MCF10A MCF10a MCF10a Michigan Cancer Foundation 10A Michigan Cancer Foundation 10A PubMed: https://www.ncbi.nlm.nih.gov/pubmed/12798140 PubMed: https://www.ncbi.nlm.nih.gov/pubmed/12798140 PubMed: https://www.ncbi.nlm.nih.gov/pubmed/1975513 PubMed: https://www.ncbi.nlm.nih.gov/pubmed/1975513 WEB: http://web.expasy.org/cellosaurus/CVCL_0598 WEB: http://web.expasy.org/cellosaurus/CVCL_0598 WEB: https://www.atcc.org/products/all/CRL-10317.aspx WEB: https://www.atcc.org/products/all/CRL-10317.aspx MCF 10A cell MCF 10A 细胞 ATCC: CRL-10317 ATCC: CRL-10317 CVCL: CVCL_0598; EFO: EFO_0001200; ChEMBL: CHEMBL3307364; HMSL: HMSL50583 CVCL: CVCL_0598; EFO: EFO_0001200; ChEMBL: CHEMBL3307364; HMSL: HMSL50583 MCF 7 MCF 7 This term is obsolete due to duplication. See: CLO_0007606. Oliver He 这个词由于重复而过时了。见：CLO_0007606. Oliver He obsolete: MCF 7 cell 过时的: MCF 7 细胞 HyperCLDB: cl3366 HyperCLDB: cl3366 MCF-7 MCF-7 This term is obsolete due to duplication. See: CLO_0007606. Oliver He 这个词由于重复而过时了。见：CLO_0007606. Oliver He obsolete: MCF-7 cell 过时的: MCF-7 细胞 HyperCLDB: cl3372 HyperCLDB: cl3372 LCL-1460 LCL-1460 ZN ZN MCF 7 cell MCF 7 细胞 MCF-7 cell MCF-7 细胞 MCF7 MCF7 WEB: http://web.expasy.org/cellosaurus/CVCL_0031 WEB: http://web.expasy.org/cellosaurus/CVCL_0031 WEB: https://en.wikipedia.org/wiki/MCF-7 WEB: https://en.wikipedia.org/wiki/MCF-7 1970年从一名69岁的白种女人身上分离出的一种永生的乳腺癌细胞系细胞。 an immortal breast cancer cell line cell isolated in 1970 from a 69-year-old Caucasian woman. disease: adenocarcinoma (mammary gland primary) 疾病：腺癌（乳腺原发性） MCF7 cell MCF7 细胞 ATCC: HTB-22 ATCC: HTB-22 CHEMBL: CHEMBL3308403 CHEMBL: CHEMBL3308403 EFO: EFO_0002082 EFO: EFO_0002082 HyperCLDB: cl3366 HyperCLDB: cl3366 HyperCLDB: cl3372 HyperCLDB: cl3372 RRID: CVCL_0031 RRID: CVCL_0031 LCL-1316 LCL-1316 MDA-MB-134-VI MDA-MB-134-VI disease: ductal carcinoma (mammary gland primary) 疾病：导管癌（乳腺原发性） MDA-MB-134-VI cell MDA-MB-134-VI 细胞 ATCC: HTB-23 ATCC: HTB-23 CHEMBL: CHEMBL3308340 CHEMBL: CHEMBL3308340 CVCL: CVCL_0617 CVCL: CVCL_0617 EFO: EFO_0001205 EFO: EFO_0001205 LCL-1916 LCL-1916 MDA-MB-157 MDA-MB-157 disease: carcinoma (mammary gland primary) 疾病：癌（乳腺原发性） MDA-MB-157 cell MDA-MB-157 细胞 ATCC: HTB-24 ATCC: HTB-24 CHEMBL: CHEMBL3308759 CHEMBL: CHEMBL3308759 CVCL: CVCL_0618 CVCL: CVCL_0618 EFO: EFO_0001206 EFO: EFO_0001206 LCL-1317 LCL-1317 MDA-MB-175-VII MDA-MB-175-VII disease: ductal carcinoma (mammary gland primary) 疾病：导管癌 MDA-MB-175-VII cell MDA-MB-175-VII 细胞 ATCC: HTB-25 ATCC: HTB-25 CHEMBL: CHEMBL3308404 CHEMBL: CHEMBL3308404 CVCL: CVCL_1400 CVCL: CVCL_1400 EFO: EFO_0001208 EFO: EFO_0001208 LCL-1461 LCL-1461 ZN, YH ZN, YH MDA-MB-231 MDA-MB-231 an immortal breast cancer cell line cell derived from a human patient with breast adenocarcinoma 从乳腺癌患者中获得的永生乳腺癌细胞系细胞 MDA-MB-231 cell MDA-MB-231 细胞 ATCC: HTB-26 ATCC: HTB-26 CHEMBL: CHEMBL3307960 CHEMBL: CHEMBL3307960 EFO: EFO_0001209 EFO: EFO_0001209 RRID: CVCL_0062 RRID: CVCL_0062 LCL-1468 LCL-1468 ZN ZN MDA-MB-361 MDA-MB-361 MDA-MB-361 cell MDA-MB-361 细胞 ATCC: HTB-27 ATCC: HTB-27 CHEMBL: CHEMBL3308405 CHEMBL: CHEMBL3308405 CVCL: CVCL_0620 CVCL: CVCL_0620 EFO: EFO_0001211 EFO: EFO_0001211 LCL-1469 LCL-1469 MDA-MB-415 MDA-MB-415 MDA-MB-415 细胞 MDA-MB-415 细胞 CHEMBL: CHEMBL3308760 CHEMBL: CHEMBL3308760 CVCL: CVCL_0621 CVCL: CVCL_0621 EFO: EFO_0001212 EFO: EFO_0001212 LCL-1307 LCL-1307 MDA-MB-435S MDA-MB-435S disease: ductal carcinoma (mammary gland primary) 疾病：导管癌（乳腺原发性） MDA-MB-435S cell MDA-MB-435S 细胞 ATCC: HTB-129 ATCC: HTB-129 LCL-1470 LCL-1470 ZN ZN MDA-MB-436 MDA-MB-436 MDA-MB-436 cell MDA-MB-436 细胞 ATCC: HTB-130 ATCC: HTB-130 LCL-1485 LCL-1485 ZN ZN MDA-MB-453 MDA-MB-453 disease: carcinoma (mammary gland primary) 疾病：癌（乳腺原发性） MDA-MB-453 cell MDA-MB-453 细胞 ATCC: HTB-131 ATCC: HTB-131 CHEMBL: CHEMBL3307596 CHEMBL: CHEMBL3307596 CVCL: CVCL_0418 CVCL: CVCL_0418 EFO: EFO_0001215 EFO: EFO_0001215 LCL-1471 LCL-1471 MDA-MB-468 MDA-MB-468 MDA-MB-468 cell MDA-MB-468 细胞 ATCC: HTB-132 ATCC: HTB-132 CHEMBL: CHEMBL3308424 CHEMBL: CHEMBL3308424 CVCL: CVCL_0419 CVCL: CVCL_0419 EFO: EFO_0001216 EFO: EFO_0001216 MDBK MDBK MDBK cell MDBK 细胞 HyperCLDB: cl3412 HyperCLDB: cl3412 MDBK (NBL-1) MDBK (NBL-1) MDBK (NBL-1) cell MDBK (NBL-1) 细胞 ATCC: CCL-22 ATCC: CCL-22 MDCK MDCK MDCK cell MDCK 细胞 HyperCLDB: cl5078 HyperCLDB: cl5078 ME ME ME cell ME 细胞 ATCC: CRL-6371 ATCC: CRL-6371 LCL-1305 LCL-1305 ME-180 ME-180 disease: epidermoid carcinoma (cervix primary) 疾病：表皮样癌（宫颈原发性） ME-180 cell ME-180 细胞 ATCC: HTB-33 ATCC: HTB-33 CHEMBL: CHEMBL3308114 CHEMBL: CHEMBL3308114 CVCL: CVCL_1401 CVCL: CVCL_1401 LCL-1110 LCL-1110 MEG-01 MEG-01 disease: megakaryoblast; chronic myelogenous leukemia 疾病：巨核细胞; 慢性髓性白血病 MEG-01 cell MEG-01 细胞 ATCC: CRL-2021 ATCC: CRL-2021 CHEMBL: CHEMBL3308539 CHEMBL: CHEMBL3308539 CVCL: CVCL_0425 CVCL: CVCL_0425 LCL-1428 LCL-1428 MG-63 MG-63 MG-63 cell MG-63 细胞 ATCC: CRL-1427 ATCC: CRL-1427 ECACC: 86051601 ECACC: 86051601 MH-S MH-S MH-S cell MH-S 细胞 ATCC: CRL-2019 ATCC: CRL-2019 LCL-1738 LCL-1738 MIA PaCa-2 MIA PaCa-2 MIA PaCa-2 cell MIA PaCa-2 细胞 ATCC: CRL-1420 ATCC: CRL-1420 CHEMBL: CHEMBL3308500 CHEMBL: CHEMBL3308500 CVCL: CVCL_0428 CVCL: CVCL_0428 EFO: EFO_0002236 EFO: EFO_0002236 HyperCLDB: cl3482 HyperCLDB: cl3482 MKN-45 MKN-45 MKN-45 cell MKN-45 细胞 HyperCLDB: cl5332 HyperCLDB: cl5332 MLTC-1 MLTC-1 disease: Leydig cell tumor 疾病：莱迪希细胞瘤 MLTC-1 cell MLTC-1 细胞 ATCC: CRL-2065 ATCC: CRL-2065 MMQ MMQ MMQ cell MMQ 细胞 ATCC: CRL-10609 ATCC: CRL-10609 M-NFS-60 M-NFS-60 disease: virus-induced myeloid leukemia 疾病：病毒诱导的骨髓性白血病 M-NFS-60 cell M-NFS-60 细胞 ATCC: CRL-1838 ATCC: CRL-1838 MNNG/HOS (Cl #5) MNNG/HOS (Cl #5) MNNG/HOS (Cl #5) cell MNNG/HOS (Cl #5) 细胞 ATCC: CRL-1547 ATCC: CRL-1547 MNNG/HOS (TE85, Clone F-5) MNNG/HOS (TE85, Clone F-5) disease: sarcoma, osteogenic 疾病：肉瘤，成骨 MNNG/HOS (TE85, Clone F-5) cell MNNG/HOS (TE85, Clone F-5) 细胞 HyperCLDB: cl3510 HyperCLDB: cl3510 LCL-1019 LCL-1019 MOLT-4 MOLT-4 MOLT-4 cell MOLT-4 细胞 ATCC: CRL-1582 ATCC: CRL-1582 CHEMBL: CHEMBL3307557 CHEMBL: CHEMBL3307557 CVCL: CVCL_0013 CVCL: CVCL_0013 EFO: EFO_0001220 EFO: EFO_0001220 MPC-11 MPC-11 disease: plasmacytoma; myeloma 疾病：浆细胞瘤;骨髓瘤 MPC-11 cell MPC-11 细胞 ATCC: CCL-167 ATCC: CCL-167 MPK MPK MPK cell MPK 细胞 ATCC: CCL-166 ATCC: CCL-166 MR1 MR1 disease: hybridoma 疾病：杂交瘤 MR1 cell MR1 细胞 ATCC: CRL-2580 ATCC: CRL-2580 MRC-5 MRC-5 MRC-5 cell MRC-5 细胞 ATCC: CCL-171 ATCC: CCL-171 HyperCLDB: cl3564 HyperCLDB: cl3564 MS1 MS1 MS1 cell MS1 细胞 ATCC: CRL-2279 ATCC: CRL-2279 LCL-1306 LCL-1306 MS751 MS751 disease: epidermoid carcinoma (cervix primary) 疾病：表皮样癌（宫颈原发性） MS751 cell MS751 细胞 ATCC: HTB-34 ATCC: HTB-34 LCL-1776 LCL-1776 MSTO-211H MSTO-211H disease: mesothelioma, biphasic (lung primary) 疾病：间皮瘤，双相（肺原发性） MSTO-211H cell MSTO-211H 细胞 ATCC: CRL-2081 ATCC: CRL-2081 CHEMBL: CHEMBL3308116 CHEMBL: CHEMBL3308116 CVCL: CVCL_1430 CVCL: CVCL_1430 EFO: EFO_0002839 EFO: EFO_0002839 MTKP 97-12 MTKP 97-12 MTKP 97-12 cell MTKP 97-12 细胞 ATCC: CRL-8985 ATCC: CRL-8985 Mv 1 Lu Mv 1 Lu Mv 1 Lu cell Mv 1 Lu 细胞 HyperCLDB: cl3604 HyperCLDB: cl3604 HyperCLDB: cl3608 HyperCLDB: cl3608 HyperCLDB: cl3609 HyperCLDB: cl3609 Mv 1 Lu (NBL-7) Mv 1 Lu (NBL-7) Mv 1 Lu (NBL-7) cell Mv 1 Lu (NBL-7) 细胞 ATCC: CCL-64 ATCC: CCL-64 LCL-2022 LCL-2022 NAMALWA NAMALWA disease: Burkitts lymphoma 疾病：伯基特淋巴瘤 NAMALWA cell NAMALWA 细胞 ATCC: CRL-1432 ATCC: CRL-1432 HyperCLDB: cl3633 HyperCLDB: cl3633 NCI-BL2009 NCI-BL2009 NCI-BL2009 cell NCI-BL2009 细胞 ATCC: CRL-5961 ATCC: CRL-5961 LCL-1609 LCL-1609 ZN, YH ZN, YH NCI-H1299 NCI-H1299 https://en.wikipedia.org/wiki/H1299 https://en.wikipedia.org/wiki/H1299 CRL-5803 CRL-5803 H1299 H1299 disease: large cell neuro- endocrine carcinoma (lung primary) 疾病：大细胞神经内分泌癌（肺原发性） NCI-H1299 cell NCI-H1299 细胞 ATCC: CRL-5803 ATCC: CRL-5803 CHEMBL: CHEMBL3308501 CHEMBL: CHEMBL3308501 CVCL: CVCL_0060 CVCL: CVCL_0060 EFO: EFO_0003043 EFO: EFO_0003043 LCL-1660 LCL-1660 NCI-H1395 NCI-H1395 NCI-H1395 cell NCI-H1395 细胞 ATCC: CRL-5868 ATCC: CRL-5868 CHEMBL: CHEMBL3308313 CHEMBL: CHEMBL3308313 CVCL: CVCL_1467 CVCL: CVCL_1467 EFO: EFO_0002252 EFO: EFO_0002252 LCL-1612 LCL-1612 ZN ZN NCI-H1650 NCI-H1650 NCI-H1650 cell NCI-H1650 细胞 CHEMBL: CHEMBL3308800 CHEMBL: CHEMBL3308800 CVCL: CVCL_1483 CVCL: CVCL_1483 EFO: EFO_0002260 EFO: EFO_0002260 LCL-1811 LCL-1811 NCI-H1688 NCI-H1688 disease: carcinoma; classic small cell lung cancer 疾病：癌；典型的小细胞肺癌 NCI-H1688 cell NCI-H1688 细胞 ATCC: CCL-257 ATCC: CCL-257 LCL-1582 LCL-1582 NCI-H1703 NCI-H1703 disease: squamous cell adenocarcinoma; non-small cell lung cancer 疾病：鳞状细胞腺癌；非小细胞肺癌 NCI-H1703 cell NCI-H1703 细胞 ATCC: CRL-5889 ATCC: CRL-5889 CHEMBL: CHEMBL3308503 CHEMBL: CHEMBL3308503 CVCL: CVCL_1490 CVCL: CVCL_1490 EFO: EFO_0002264 EFO: EFO_0002264 LCL-1663 LCL-1663 NCI-H1975 NCI-H1975 NCI-H1975 cell NCI-H1975 细胞 ATCC: CRL-5908 ATCC: CRL-5908 CHEMBL: CHEMBL3307601 CHEMBL: CHEMBL3307601 CVCL: CVCL_1511 CVCL: CVCL_1511 EFO: EFO_0002271 EFO: EFO_0002271 LCL-1647 LCL-1647 ZN ZN NCI-H2009 NCI-H2009 NCI-H2009 cell NCI-H2009 细胞 CHEMBL: CHEMBL3308459 CHEMBL: CHEMBL3308459 CVCL: CVCL_1514 CVCL: CVCL_1514 EFO: EFO_0002273 EFO: EFO_0002273 LCL-1650 LCL-1650 ZN ZN NCI-H2087 NCI-H2087 NCI-H2087 cell NCI-H2087 细胞 CHEMBL: CHEMBL3308770 CHEMBL: CHEMBL3308770 CVCL: CVCL_1524 CVCL: CVCL_1524 EFO: EFO_0002277 EFO: EFO_0002277 LCL-1847 LCL-1847 NCI-H209 NCI-H209 disease: carcinoma; small cell lung cancer (lung primary) 疾病：癌；小细胞肺癌（肺原发性） NCI-H209 cell NCI-H209 细胞 ATCC: HTB-172 ATCC: HTB-172 CHEMBL: CHEMBL3308293 CHEMBL: CHEMBL3308293 CVCL: CVCL_1525 CVCL: CVCL_1525 LCL-1590 LCL-1590 NCI-H2170 NCI-H2170 disease: squamous cell carcinoma 疾病：鳞状细胞癌 NCI-H2170 cell NCI-H2170 细胞 ATCC: CRL-5928 ATCC: CRL-5928 CHEMBL: CHEMBL3308460 CHEMBL: CHEMBL3308460 CVCL: CVCL_1535 CVCL: CVCL_1535 EFO: EFO_0002281 EFO: EFO_0002281 LCL-1853 LCL-1853 NCI-H2227 NCI-H2227 disease: carcinoma; small cell lung cancer 疾病：癌；小细胞肺癌 NCI-H2227 cell NCI-H2227 细胞 ATCC: CRL-5934 ATCC: CRL-5934 CHEMBL: CHEMBL3308321 CHEMBL: CHEMBL3308321 CVCL: CVCL_1542 CVCL: CVCL_1542 LCL-1597 LCL-1597 ZN ZN NCI-H226 NCI-H226 disease: squamous cell carcinoma; mesothelioma (lung primary) 疾病：鳞状细胞癌；间皮瘤（肺原发性） NCI-H226 cell NCI-H226 细胞 ATCC: CRL-5826 ATCC: CRL-5826 CHEMBL: CHEMBL3307697 CHEMBL: CHEMBL3307697 CVCL: CVCL_1544 CVCL: CVCL_1544 EFO: EFO_0002285 EFO: EFO_0002285 LCL-1619 LCL-1619 NCI-H23 NCI-H23 NCI-H23 cell NCI-H23 细胞 ATCC: CRL-5800 ATCC: CRL-5800 CHEMBL: CHEMBL3307750 CHEMBL: CHEMBL3307750 CVCL: CVCL_1547 CVCL: CVCL_1547 EFO: EFO_0002286 EFO: EFO_0002286 LCL-1230 LCL-1230 NCI-H2452 NCI-H2452 disease: mesothelioma 疾病：间皮瘤 NCI-H2452 cell NCI-H2452 细胞 ATCC: CRL-5946 ATCC: CRL-5946 CHEMBL: CHEMBL3308580 CHEMBL: CHEMBL3308580 CVCL: CVCL_1553 CVCL: CVCL_1553 NCI-H292 NCI-H292 disease: mucoepidermoid pulmonary carcinoma 疾病：黏液表皮样肺癌 NCI-H292 cell NCI-H292 细胞 ATCC: CRL-1848 ATCC: CRL-1848 NCI-H295R NCI-H295R NCI-H295R cell NCI-H295R 细胞 ATCC: CRL-2128 ATCC: CRL-2128 LCL-1671 LCL-1671 ZN ZN NCI-H358 NCI-H358 disease: alveolus); bronchioalveolar carcinoma; non-small cell lung cancer 疾病：（肺泡）；支气管肺泡癌；非小细胞肺癌 NCI-H358 cell NCI-H358 细胞 ATCC: CRL-5807 ATCC: CRL-5807 CHEMBL: CHEMBL3307543 CHEMBL: CHEMBL3307543 CVCL: CVCL_1559 CVCL: CVCL_1559 EFO: EFO_0002291 EFO: EFO_0002291 LCL-1911 LCL-1911 NCI-H441 NCI-H441 disease: papillary adeno- carcinoma (lung primary) 疾病：乳头状腺癌（肺原发性） NCI-H441 cell NCI-H441 细胞 ATCC: HTB-174 ATCC: HTB-174 CHEMBL: CHEMBL3308121 CHEMBL: CHEMBL3308121 CVCL: CVCL_1561 CVCL: CVCL_1561 EFO: EFO_0002292 EFO: EFO_0002292 LCL-1855 LCL-1855 NCI-H446 NCI-H446 disease: carcinoma; small cell lung cancer (lung primary) 疾病：癌；小细胞肺癌（肺原发性） NCI-H446 cell NCI-H446 细胞 ATCC: HTB-171 ATCC: HTB-171 CHEMBL: CHEMBL3307760 CHEMBL: CHEMBL3307760 CVCL: CVCL_1562 CVCL: CVCL_1562 EFO: EFO_0002293 EFO: EFO_0002293 LCL-1784 LCL-1784 NCI-H460 NCI-H460 QL, YH QL, YH disease: large cell carci- noma (lung primary) 疾病：大细胞癌（肺原发性） NCI-H460 cell NCI-H460 细胞 ATCC: HTB-177 ATCC: HTB-177 CHEMBL: CHEMBL3307677 CHEMBL: CHEMBL3307677 CVCL: CVCL_0459 CVCL: CVCL_0459 EFO: EFO_0003044 EFO: EFO_0003044 NCI-H508 NCI-H508 NCIH508 NCIH508 disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 NCI-H508 cell NCI-H508 细胞 ATCC: CCL-253 ATCC: CCL-253 LCL-1591 LCL-1591 NCI-H520 NCI-H520 disease: squamous cell carcinoma 疾病：鳞状细胞癌 NCI-H520 cell NCI-H520 细胞 ATCC: HTB-182 ATCC: HTB-182 LCL-1857 LCL-1857 NCI-H524 NCI-H524 disease: carcinoma; variant small cell lung cancer (lung primary) 疾病：癌；变异型小细胞肺癌（肺原发性） NCI-H524 cell NCI-H524 细胞 ATCC: CRL-5831 ATCC: CRL-5831 CHEMBL: CHEMBL3308296 CHEMBL: CHEMBL3308296 CVCL: CVCL_1568 CVCL: CVCL_1568 EFO: EFO_0002296 EFO: EFO_0002296 LCL-1786 LCL-1786 NCI-H596 NCI-H596 NCIH596 NCIH596 NCI-H596 cell NCI-H596 细胞 ATCC: HTB-178 ATCC: HTB-178 LCL-1785 LCL-1785 NCI-H661 NCI-H661 disease: carcinoma; large cell lung cancer (lung primary) 疾病：癌；大细胞肺癌（肺原发性） NCI-H661 cell NCI-H661 细胞 ATCC: HTB-183 ATCC: HTB-183 CHEMBL: CHEMBL3308139 CHEMBL: CHEMBL3308139 CVCL: CVCL_1577 CVCL: CVCL_1577 EFO: EFO_0002299 EFO: EFO_0002299 LCL-1859 LCL-1859 NCI-H69 NCI-H69 disease: carcinoma; small cell lung cancer (lung primary) 疾病：癌；小细胞肺癌（肺原发性） NCI-H69 cell NCI-H69 细胞 ATCC: HTB-119 ATCC: HTB-119 CHEMBL: CHEMBL3307712 CHEMBL: CHEMBL3307712 CVCL: CVCL_1579 CVCL: CVCL_1579 EFO: EFO_0002823 EFO: EFO_0002823 LCL-1339 LCL-1339 NCI-H716 NCI-H716 disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 NCI-H716 cell NCI-H716 细胞 ATCC: CCL-251 ATCC: CCL-251 CHEMBL: CHEMBL3308297 CHEMBL: CHEMBL3308297 CVCL: CVCL_1581 CVCL: CVCL_1581 EFO: EFO_0002301 EFO: EFO_0002301 Sanger: COSMIC ID:908458 Sanger: COSMIC ID:908458 LCL-1623 LCL-1623 ZN ZN NCI-H838 NCI-H838 NCI-H838 cell NCI-H838 细胞 ATCC: CRL-5844 ATCC: CRL-5844 CHEMBL: CHEMBL3308122 CHEMBL: CHEMBL3308122 CVCL: CVCL_1594 CVCL: CVCL_1594 EFO: EFO_0002307 EFO: EFO_0002307 NCI-H929 NCI-H929 disease: plasmacytoma; myeloma 疾病：浆细胞瘤；骨髓瘤 NCI-H929 cell NCI-H929 细胞 ATCC: CRL-9068 ATCC: CRL-9068 LCL-1886 LCL-1886 NCI-N87 NCI-N87 disease: gastric carcinoma (stomach primary) 疾病：胃癌（胃原发性） NCI-N87 cell NCI-N87 细胞 ATCC: CRL-5822 ATCC: CRL-5822 CHEMBL: CHEMBL3307326 CHEMBL: CHEMBL3307326 CVCL: CVCL_1603 CVCL: CVCL_1603 EFO: EFO_0002841 EFO: EFO_0002841 NCTC clone 1469 NCTC clone 1469 NCTC clone 1469 cell NCTC clone 1469 细胞 ATCC: CCL-9.1 ATCC: CCL-9.1 NCTC clone 929 NCTC clone 929 NCTC clone 929 cell NCTC clone 929 细胞 ATCC: CCL-1 ATCC: CCL-1 Neuro-2a Neuro-2a Neuro-2a cell Neuro-2a 细胞 ATCC: CCL-131 ATCC: CCL-131 HyperCLDB: cl3684 HyperCLDB: cl3684 HyperCLDB: cl3685 HyperCLDB: cl3685 HyperCLDB: cl4956 HyperCLDB: cl4956 NG108-15 NG108-15 disease: glial cell; neuron; glioblastoma; neuro- blastoma (hybrid) 疾病：胶质细胞；神经元；胶质母细胞瘤；神经母细胞瘤（杂交） NG108-15 cell NG108-15 细胞 ATCC: HB-12317 ATCC: HB-12317 NIH-3T3 NIH-3T3 NIH-3T3 cell NIH-3T3 细胞 ATCC: CRL-1658 ATCC: CRL-1658 HyperCLDB: cl3710 HyperCLDB: cl3710 HyperCLDB: cl3711 HyperCLDB: cl3711 MeSH: D041681 MeSH: D041681 NK-92 NK-92 disease: IL-2 dependent, cytotoxic to a wide range of malignant cells 疾病：IL-2依赖性，对广泛的恶性细胞具有细胞毒性 NK-92 cell NK-92 细胞 ATCC: CRL-2407 ATCC: CRL-2407 LCL-1127 LCL-1127 NK-92MI NK-92MI disease: IL-2 independent, cytotoxic to a wide range of malignant cells 疾病：IL-2非依赖性，对广泛的恶性细胞具有细胞毒性 NK-92MI cell NK-92MI 细胞 ATCC: CRL-2408 ATCC: CRL-2408 NR8383 NR8383 NR8383 cell NR8383 细胞 ATCC: CRL-2192 ATCC: CRL-2192 NRK NRK NRK cell NRK 细胞 ATCC: CRL-6509 ATCC: CRL-6509 NRK 49F NRK 49F NRK 49F cell NRK 49F 细胞 HyperCLDB: cl3726 HyperCLDB: cl3726 NRK-49F NRK-49F NRK-49F cell NRK-49F 细胞 ATCC: CRL-1570 ATCC: CRL-1570 NRK-52E NRK-52E NRK-52E cell NRK-52E 细胞 ATCC: CRL-1571 ATCC: CRL-1571 NT2-D1 NT2-D1 NT2/D1 NT2/D1 NT2D1 NT2D1 NTERA-2 cl.D1 NTERA-2 cl.D1 NTERA-2 clone D1 NTERA-2 clone D1 NTERA-2/D1 NTERA-2/D1 NTERA-2cl.D1 NTERA-2cl.D1 NTERA2-D1 NTERA2-D1 NTERA2-cloneD1 NTERA2-cloneD1 NTERA2/D1 NTERA2/D1 NTERA2D1 NTERA2D1 NTera 2/cl.D1 NTera 2/cl.D1 NTera-2D1 NTera-2D1 NTera2 cl.D1 NTera2 cl.D1 NTera2/D1 NTera2/D1 NTera2D1 NTera2D1 Nt2D1 Nt2D1 Ntera2/D1 Ntera2/D1 WEB: http://web.expasy.org/cellosaurus/CVCL_3407 WEB: http://web.expasy.org/cellosaurus/CVCL_3407 WEB: https://www.atcc.org/Products/All/CRL-1973.aspx WEB: https://www.atcc.org/Products/All/CRL-1973.aspx The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line (see ATCC HTB-106). The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line. Ref: https://www.atcc.org/Products/All/CRL-1973.aspx#characteristics epithelial-like,differentiation changes phenotype. https://www.atcc.org/Products/All/CRL-1973.aspx 上皮样，分化改变表型。 https://www.atcc.org/Products/All/CRL-1973.aspx 亲代NTERA-2细胞系于1980年由Tera-2细胞系的裸鼠异种移植物建立（参见ATCC HTB-106）。 NTERA-2 cl.D1细胞系是通过克隆NTERA-2细胞系衍生的多能人类睾丸胚胎癌细胞系。 Ref: https://www.atcc.org/Products/All/CRL-1973.aspx#characteristics NTERA-2 cl.D1 cell NTERA-2 cl.D1 细胞 ATCC: CRL-1973 ATCC: CRL-1973 HyperCLDB: cl5088 HyperCLDB: cl5088 OK OK OK cell OK 细胞 ATCC: CRL-1840 ATCC: CRL-1840 OKT 11 OKT 11 disease: hybridoma 疾病：杂交瘤 OKT 11 cell OKT 11 细胞 ATCC: CRL-8027 ATCC: CRL-8027 OKT 3 OKT 3 disease: hybridoma 疾病：杂交瘤 OKT 3 cell OKT 3 细胞 ATCC: CRL-8001 ATCC: CRL-8001 OP9 OP9 disease: bone marrow, stroma 疾病：骨髓，间质 OP9 cell OP9 细胞 ATCC: CRL-2749 ATCC: CRL-2749 OVCAR-3 OVCAR-3 OVCAR-3 cell OVCAR-3 细胞 HyperCLDB: cl3780 HyperCLDB: cl3780 P19 P19 disease: teratocarcinoma; embryonic carcinoma 疾病：畸胎瘤；胚胎癌 P19 cell P19 细胞 ATCC: CRL-1825 ATCC: CRL-1825 P3/NSI/1-Ag4-1 (NS-1) P3/NSI/1-Ag4-1 (NS-1) disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 P3/NSI/1-Ag4-1 (NS-1) cell P3/NSI/1-Ag4-1 (NS-1) 细胞 ATCC: TIB-18 ATCC: TIB-18 HyperCLDB: cl3825 HyperCLDB: cl3825 P388 P388 disease: leukemia 疾病：白血病 P388 cell P388 细胞 HyperCLDB: cl3829 HyperCLDB: cl3829 P388D subscript(1) P388D subscript(1) disease: monocyte; lymphoma 疾病：单核细胞；淋巴瘤 P388D subscript(1) cell P388D subscript(1) 细胞 ATCC: TIB-63 ATCC: TIB-63 P388D1 (IL-1) P388D1 (IL-1) P388D1 (IL-1) cell P388D1 (IL-1) 细胞 HyperCLDB: cl3835 HyperCLDB: cl3835 HyperCLDB: cl5344 HyperCLDB: cl5344 P3X63Ag8 P3X63Ag8 disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 P3X63Ag8 cell P3X63Ag8 细胞 ATCC: TIB-9 ATCC: TIB-9 P3X63Ag8.653 P3X63Ag8.653 disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 P3X63Ag8.653 cell P3X63Ag8.653 细胞 ATCC: CRL-1580 ATCC: CRL-1580 Oliver He Oliver He P815 P815 WEB: http://www.ncbi.nlm.nih.gov/pubmed/18432777 WEB: http://www.ncbi.nlm.nih.gov/pubmed/18432777 immortal mouse mast cell line cell that was derived from some lymph node mast cell from a /2 mouse with mast cell neoplasm 永生的小鼠肥大细胞系细胞，其衍生自一些淋巴结肥大细胞，这些肥大细胞来自一只具有肥大细胞瘤的/2小鼠 disease: mastocytoma 疾病：肥大细胞瘤 P815 cell P815 细胞 ATCC: TIB-64 ATCC: TIB-64 RRID: CVCL_2154 RRID: CVCL_2154 LCL-1416 LCL-1416 ZN ZN PA-1 PA-1 disease: teratocarcinoma (ovary primary) 疾病：畸胎瘤（卵巢原发性） PA-1 cell PA-1 细胞 ATCC: CRL-1572 ATCC: CRL-1572 CHEMBL: CHEMBL3308812 CHEMBL: CHEMBL3308812 CVCL: CVCL_0479 CVCL: CVCL_0479 PA317 PA317 disease: amphotropic retroviral packaging line 疾病：兼嗜性逆转录病毒包装细胞系 PA317 cell PA317 细胞 ATCC: CRL-9078 ATCC: CRL-9078 LCL-1726 LCL-1726 PANC-1 PANC-1 WEB: https://www.atcc.org/Products/All/CRL-1469.aspx WEB: https://www.atcc.org/Products/All/CRL-1469.aspx disease: epithelioid carcinoma 疾病：上皮样癌 PANC-1 cell PANC-1 细胞 ATCC: CRL-1469 ATCC: CRL-1469 CHEMBL: CHEMBL3307545 CHEMBL: CHEMBL3307545 CVCL: CVCL_0480 CVCL: CVCL_0480 EFO: EFO_0002713 EFO: EFO_0002713 PC 61 5.3 PC 61 5.3 disease: hybridoma 疾病：杂交瘤 PC 61 5.3 cell PC 61 5.3 细胞 ATCC: TIB-222 ATCC: TIB-222 PC-12 PC-12 disease: pheochromocytoma 疾病：嗜铬细胞瘤 PC-12 cell PC-12 细胞 ATCC: CRL-1721 ATCC: CRL-1721 MeSH: D016716 MeSH: D016716 LCL-1299 LCL-1299 Oliver He Oliver He an immortal human prostate epithelium-derived cell line cell that was established in 1979 from bone metastasis of grade IV of prostate cancer in a 62-year-old Caucasian male. 一种永生的人前列腺上皮衍生细胞系细胞，建立于1979年，从一个62岁的白人男性前列腺癌四级骨转移。 PC-3 PC-3 WEB: https://en.wikipedia.org/wiki/PC3 WEB: https://en.wikipedia.org/wiki/PC3 PC3 PC3 PC-3 cell PC-3 细胞 ATCC: CRL-1435 ATCC: CRL-1435 CHEMBL: CHEMBL3307570 CHEMBL: CHEMBL3307570 EFO: EFO_0002074 EFO: EFO_0002074 RRID: CVCL_0035 RRID: CVCL_0035 PG PG PG cell PG 细胞 HyperCLDB: cl5107 HyperCLDB: cl5107 PG-4 PG-4 PG-4 cell PG-4 细胞 HyperCLDB: cl3898 HyperCLDB: cl3898 PG-4 (S+L–) PG-4 (S+L–) PG-4 (S+L–) cell PG-4 (S+L–) 细胞 ATCC: CRL-2032 ATCC: CRL-2032 HyperCLDB: cl3899 HyperCLDB: cl3899 pgsA-745 pgsA-745 disease: xylosyltransferase I deficient 疾病：木糖基转移酶I缺陷 pgsA-745 cell pgsA-745 细胞 ATCC: CRL-2242 ATCC: CRL-2242 PK(15) PK(15) PK(15) cell PK(15) 细胞 ATCC: CCL-33 ATCC: CCL-33 PK136 PK136 disease: hybridoma 疾病：杂交瘤 PK136 cell PK136 细胞 ATCC: HB-191 ATCC: HB-191 LCL-1942 LCL-1942 PLC/PRF/5 PLC/PRF/5 disease: hepatoma 疾病：肝癌 PLC/PRF/5 cell PLC/PRF/5 细胞 ATCC: CRL-8024 ATCC: CRL-8024 ECACC: 85061113 ECACC: 85061113 Pt K1 (NBL-3) Pt K1 (NBL-3) Pt K1 (NBL-3) cell Pt K1 (NBL-3) 细胞 ATCC: CCL-35 ATCC: CCL-35 R 1610 R 1610 R 1610 cell R 1610 细胞 ATCC: CRL-1657 ATCC: CRL-1657 R1 R1 disease: derived from 129 substrain 疾病：由129亚株衍生而来 R1 cell R1 细胞 ATCC: SCRC-1011 ATCC: SCRC-1011 R1/E R1/E disease: embryonic stem cell 疾病：胚胎干细胞 R1/E cell R1/E 细胞 ATCC: SCRC-1036 ATCC: SCRC-1036 RAG RAG disease: renal adenocarcinoma 疾病：肾腺癌 RAG cell RAG 细胞 ATCC: CCL-142 ATCC: CCL-142 LCL-2025 LCL-2025 Raji Raji disease: Burkitts lymphoma 疾病：伯基特淋巴瘤 Raji cell Raji 细胞 ATCC: CCL-86 ATCC: CCL-86 CHEMBL: CHEMBL3307663 CHEMBL: CHEMBL3307663 CVCL: CVCL_0511 CVCL: CVCL_0511 EFO: EFO_0002324 EFO: EFO_0002324 HyperCLDB: cl4091 HyperCLDB: cl4091 HyperCLDB: cl4092 HyperCLDB: cl4092 RAMOS RAMOS RAMOS cell RAMOS 细胞 HyperCLDB: cl4096 HyperCLDB: cl4096 LCL-1097 LCL-1097 Ramos (RA 1) Ramos (RA 1) disease: Burkitts lymphoma (American) 疾病：伯基特淋巴瘤（美国） Ramos (RA 1) cell Ramos (RA 1) 细胞 ATCC: CRL-1596 ATCC: CRL-1596 HyperCLDB: cl4098 HyperCLDB: cl4098 RAW 264.7 RAW 264.7 disease: macrophage; AMLV-induced tumor 疾病：巨噬细胞； AMLV诱导的肿瘤 RAW 264.7 cell RAW 264.7 细胞 ATCC: TIB-71 ATCC: TIB-71 RBL-1 RBL-1 disease: basophil; leukemia 疾病：嗜碱性粒细胞；白血病 RBL-1 cell RBL-1 细胞 ATCC: CRL-1378 ATCC: CRL-1378 RBL-2H3 RBL-2H3 disease: basophil; leukemia 疾病：嗜碱性粒细胞；白血病 RBL-2H3 cell RBL-2H3 细胞 ATCC: CRL-2256 ATCC: CRL-2256 LCL-1411 LCL-1411 ZN ZN RD RD RD cell RD 细胞 ATCC: CCL-136 ATCC: CCL-136 CHEMBL: CHEMBL3307547 CHEMBL: CHEMBL3307547 CVCL: CVCL_1649 CVCL: CVCL_1649 EFO: EFO_0002315 EFO: EFO_0002315 LCL-1039 LCL-1039 REH REH REH cell REH 细胞 CHEMBL: CHEMBL3308823 CHEMBL: CHEMBL3308823 CVCL: CVCL_1650 CVCL: CVCL_1650 HyperCLDB: cl4132 HyperCLDB: cl4132 RF/6A RF/6A RF/6A cell RF/6A 细胞 ATCC: CRL-1780 ATCC: CRL-1780 RIN-m5F RIN-m5F disease: insulinoma; produces insulin and L-dopa-decarboxylase but not somatostatin 疾病：胰岛素瘤；产生胰岛素和左旋多巴脱羧酶，但不生长抑制素 RIN-m5F cell RIN-m5F 细胞 ATCC: CRL-11605 ATCC: CRL-11605 RK13 RK13 RK13 cell RK13 细胞 HyperCLDB: cl4155 HyperCLDB: cl4155 LCL-1168 LCL-1168 RKO RKO disease: carcinoma; control for RKO-E6 and RKO-AS45-1 cells 疾病：癌；控制RKO-E6和RKO-AS45-1细胞 RKO cell RKO 细胞 ATCC: CRL-2577 ATCC: CRL-2577 CHEMBL: CHEMBL3307731 CHEMBL: CHEMBL3307731 CVCL: CVCL_0504 CVCL: CVCL_0504 EFO: EFO_0001232 EFO: EFO_0001232 Sanger: COSMIC ID:909698 Sanger: COSMIC ID:909698 RKO-AS45-1 RKO-AS45-1 disease: carcinoma; apoptosis model; overexpression of GADD45 疾病：癌；凋亡模型； GADD45过表达 RKO-AS45-1 cell RKO-AS45-1 细胞 ATCC: CRL-2579 ATCC: CRL-2579 RKO-E6 RKO-E6 disease: carcinoma; apoptosis model; negligible p53 expression 疾病：癌；凋亡模型；微量p53表达 RKO-E6 cell RKO-E6 细胞 ATCC: CRL-2578 ATCC: CRL-2578 LCL-1506 LCL-1506 ZN ZN RL95-2 RL95-2 RL95-2 cell RL95-2 细胞 ATCC: CRL-1671 ATCC: CRL-1671 ATCC: CRL-1671,COSMIC ID:930082; ATCC CRL-1671 ATCC: CRL-1671,COSMIC ID:930082; ATCC CRL-1671 CHEMBL: CHEMBL3308843 CHEMBL: CHEMBL3308843 CVCL: CVCL_0505 CVCL: CVCL_0505 RMC RMC RMC cell RMC 细胞 ATCC: CRL-2573 ATCC: CRL-2573 LCL-2049 LCL-2049 RPMI 8226 RPMI 8226 RPMI 8226 cell RPMI 8226 细胞 ATCC: CCL-155 ATCC: CCL-155 HyperCLDB: cl5355 HyperCLDB: cl5355 RSC96 RSC96 disease: immortalized spontaneously 疾病：自发永生化 RSC96 cell RSC96 细胞 ATCC: CRL-2765 ATCC: CRL-2765 LCL-1717 LCL-1717 RT4 RT4 disease: transitional cell papilloma 疾病：移行细胞乳头状瘤 RT4 cell RT4 细胞 ATCC: HTB-2 ATCC: HTB-2 ECACC: 91091914 ECACC: 91091914 HyperCLDB: cl5358 HyperCLDB: cl5358 RWPE-1 RWPE-1 disease: transfected with Ki-MSV 疾病：转染Ki-MSV RWPE-1 cell RWPE-1 细胞 ATCC: CRL-11609 ATCC: CRL-11609 RWPE-2 RWPE-2 disease: transfected with HPV-18 and Ki-MSV 疾病：转染HPV-18和Ki-MSV RWPE-2 cell RWPE-2 细胞 ATCC: CRL-11610 ATCC: CRL-11610 S1E4 S1E4 disease: hybridoma 疾病：杂交瘤 S1E4 cell S1E4 细胞 ATCC: HB-8332 ATCC: HB-8332 S2 S2 S2 cell S2 细胞 HyperCLDB: cl4242 HyperCLDB: cl4242 S49 (Thy-1-a) S49 (Thy-1-a) S49 (Thy-1-a) cell S49 (Thy-1-a) 细胞 ATCC: TIB-36 ATCC: TIB-36 LCL-1430 LCL-1430 Saos-2 Saos-2 Saos-2 cell Saos-2 细胞 ATCC: HTB-85 ATCC: HTB-85 CHEMBL: CHEMBL3307737 CHEMBL: CHEMBL3307737 CVCL: CVCL_0548 CVCL: CVCL_0548 HyperCLDB: cl4251 HyperCLDB: cl4251 HyperCLDB: cl4252 HyperCLDB: cl4252 LCL-1944 LCL-1944 SC-1 SC-1 disease: EBV transformed 疾病：EBV转化 SC-1 cell SC-1 细胞 ATCC: CRL-8756 ATCC: CRL-8756 CRL-8756 CRL-8756 LCL-1540 LCL-1540 SCaBER SCaBER disease: transitional cell carcinoma 疾病：移行细胞癌 SCaBER cell SCaBER 细胞 ATCC: HTB-3 ATCC: HTB-3 CHEMBL: CHEMBL3307437 CHEMBL: CHEMBL3307437 CVCL: CVCL_3599 CVCL: CVCL_3599 EFO: EFO_0002325 EFO: EFO_0002325 LCL-1222 LCL-1222 SCC-15 SCC-15 disease: squamous cell carcinoma 疾病：鳞状细胞癌 SCC-15 cell SCC-15 细胞 ATCC: CRL-1623 ATCC: CRL-1623 CHEMBL: CHEMBL3307438 CHEMBL: CHEMBL3307438 CVCL: CVCL_1681 CVCL: CVCL_1681 SF-21 SF-21 SF-21 cell SF-21 细胞 HyperCLDB: cl2906 HyperCLDB: cl2906 Sf9 Sf9 Sf9 cell Sf9 细胞 ATCC: CRL-1711 ATCC: CRL-1711 SH2 SH2 disease: hybridoma 疾病：杂交瘤 SH2 cell SH2 细胞 ATCC: HB-10743 ATCC: HB-10743 SH3 SH3 disease: hybridoma 疾病：杂交瘤 SH3 cell SH3 细胞 ATCC: HB-10744 ATCC: HB-10744 LCL-2000 LCL-2000 SH-SY5Y SH-SY5Y https://www.atcc.org/en/Products/Cells_and_Microorganisms/By_Tissue/Bone_Marrow/CRL-2266.aspx https://www.atcc.org/en/Products/Cells_and_Microorganisms/By_Tissue/Bone_Marrow/CRL-2266.aspx SHSY5Y SHSY5Y SH-SY5Y cell SH-SY5Y 细胞 ATCC: CRL-2266 ATCC: CRL-2266 LCL-1542 LCL-1542 SiHa SiHa disease: squamous cell carcinoma 疾病：鳞状细胞癌 SiHa cell SiHa 细胞 ATCC: HTB-35 ATCC: HTB-35 CHEMBL: CHEMBL3307968 CHEMBL: CHEMBL3307968 CVCL: CVCL_0032 CVCL: CVCL_0032 EFO: EFO_0002379 EFO: EFO_0002379 LCL-1475 LCL-1475 ZN ZN SK-BR-3 SK-BR-3 SK-BR-3 cell SK-BR-3 细胞 ATCC: HTB-30 ATCC: HTB-30 CHEMBL: CHEMBL3308062 CHEMBL: CHEMBL3308062 CVCL: CVCL_0033 CVCL: CVCL_0033 EFO: EFO_0001236 EFO: EFO_0001236 LCL-1184 LCL-1184 SK-CO-1 SK-CO-1 disease: colorectal adenocarcinoma (colon primary) 疾病：结肠直肠腺癌（结肠原发性） SK-CO-1 cell SK-CO-1 细胞 ATCC: HTB-39 ATCC: HTB-39 CHEMBL: CHEMBL3308784 CHEMBL: CHEMBL3308784 CVCL: CVCL_0626 CVCL: CVCL_0626 LCL-1941 LCL-1941 SK-HEP-1 SK-HEP-1 SK-HEP-1 cell SK-HEP-1 细胞 ATCC: HTB-52 ATCC: HTB-52 LCL-1659 LCL-1659 SK-LU-1 SK-LU-1 SKLU1 SKLU1 SK-LU-1 cell SK-LU-1 细胞 ATCC: HTB-57 ATCC: HTB-57 CHEMBL: CHEMBL3307447 CHEMBL: CHEMBL3307447 CVCL: CVCL_0629 CVCL: CVCL_0629 ECACC: 93120835 ECACC: 93120835 LCL-1265 LCL-1265 SK-MEL-1 SK-MEL-1 disease: malignant melanoma (skin primary) 疾病：恶性黑色素瘤（皮肤原发性） SK-MEL-1 cell SK-MEL-1 细胞 ATCC: HTB-67 ATCC: HTB-67 CHEMBL: CHEMBL3308365 CHEMBL: CHEMBL3308365 CVCL: CVCL_0068 CVCL: CVCL_0068 EFO: EFO_0002332 EFO: EFO_0002332 LCL-1583 LCL-1583 SK-MES SK-MES http://web.expasy.org/cellosaurus/CVCL_0630 SK MES 1 SK-MES disease: carcinoma, squamous cell 疾病：癌，鳞状细胞 SK-MES-1 cell SK-MES-1 细胞 ATCC: HTB-58 ATCC: HTB-58 HyperCLDB: cl4325 HyperCLDB: cl4325 RRID:CVCL_0630 LCL-1980 LCL-1980 SK-N-BE(2) SK-N-BE(2) SK-N-BE(2) cell SK-N-BE(2) 细胞 ATCC: CRL-2271 ATCC: CRL-2271 ECACC: 95011815 ECACC: 95011815 LCL-1802 LCL-1802 SK-NEP-1 SK-NEP-1 disease: Wilmstumor (kidney primary) 疾病：肾母细胞瘤（肾原发性） SK-NEP-1 cell SK-NEP-1 细胞 ATCC: HTB-48 ATCC: HTB-48 CHEMBL: CHEMBL3308141 CHEMBL: CHEMBL3308141 CVCL: CVCL_0631 CVCL: CVCL_0631 EFO: EFO_0002337 EFO: EFO_0002337 SK-N-MC SK-N-MC disease: neuroepithelioma (brain primary) 疾病：神经上皮瘤（大脑） SK-N-MC cell SK-N-MC 细胞 ATCC: HTB-10 ATCC: HTB-10 LCL-1971 LCL-1971 SK-N-SH SK-N-SH SK-N-SH cell SK-N-SH 细胞 ATCC: HTB-11 ATCC: HTB-11 CHEMBL: CHEMBL3307745 CHEMBL: CHEMBL3307745 CVCL: CVCL_0531 CVCL: CVCL_0531 EFO: EFO_0003072 EFO: EFO_0003072 LCL-1517 LCL-1517 SK-OV-3 SK-OV-3 SK-OV-3 cell SK-OV-3 细胞 ATCC: HTB-77 ATCC: HTB-77 CHEMBL: CHEMBL3307746 CHEMBL: CHEMBL3307746 CVCL: CVCL_0532 CVCL: CVCL_0532 ECACC: 91091004 ECACC: 91091004 EFO: EFO_0002340 EFO: EFO_0002340 SMC-1 SMC-1 SMC-1 cell SMC-1 细胞 HyperCLDB: cl4369 HyperCLDB: cl4369 LCL-1890 LCL-1890 SNU-5 SNU-5 disease: gastric carcinoma (stomach primary) 疾病：胃癌（胃原发性） SNU-5 cell SNU-5 细胞 ATCC: CRL-5973 ATCC: CRL-5973 CHEMBL: CHEMBL3307458 CHEMBL: CHEMBL3307458 CVCL: CVCL_0078 CVCL: CVCL_0078 EFO: EFO_0002351 EFO: EFO_0002351 SP2/0-Ag14 SP2/0-Ag14 SP2/0-Ag14 cell SP2/0-Ag14 细胞 HyperCLDB: cl4384 HyperCLDB: cl4384 SP2/0-AG14 SP2/0-AG14 SP2/0-AG14 cell SP2/0-AG14 细胞 HyperCLDB: cl4386 HyperCLDB: cl4386 Sp2/0-Ag14 Sp2/0-Ag14 disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 Sp2/0-Ag14 cell Sp2/0-Ag14 细胞 ATCC: CRL-1581 ATCC: CRL-1581 Sp2/0-Ag14 Sp2/0-Ag14 Sp2/0-Ag14 cell Sp2/0-Ag14 细胞 ATCC: CRL-8287 ATCC: CRL-8287 ST ST ST cell ST 细胞 ATCC: CRL-1746 ATCC: CRL-1746 STO STO STO cell STO 细胞 ATCC: CRL-1503 ATCC: CRL-1503 LCL-1041 LCL-1041 Oliver He Oliver He SUP-B15 SUP-B15 WEB: http://www.atcc.org/products/all/CRL-1929.aspx WEB: http://www.atcc.org/products/all/CRL-1929.aspx WEB: http://www.atcc.org/~/ps/CRL-1929.ashx WEB: http://www.atcc.org/~/ps/CRL-1929.ashx an immortal human B cell line cell that is derived from a 8-year-old Caucasian male human patient with an acute lymphoblastic leukemia. 源自一个8岁的患有急性淋巴细胞白血病的白人男性患者的永生人B细胞系细胞。 CRL-1929 CRL-1929 SUP-B15 cell SUP-B15 细胞 ATCC: CRL-1929 ATCC: CRL-1929 RRID: CVCL_0103 RRID: CVCL_0103 Super Dome Super Dome Super Dome cell Super Dome 细胞 ATCC: CRL-2286 ATCC: CRL-2286 Super Tube Super Tube Super Tube cell Super Tube 细胞 ATCC: CRL-2285 ATCC: CRL-2285 SV40 MES 13 SV40 MES 13 SV40 MES 13 cell SV40 MES 13 细胞 ATCC: CRL-1927 ATCC: CRL-1927 SV-HUC-1 SV-HUC-1 SV-HUC-1 cell SV-HUC-1 细胞 ATCC: CRL-9520 ATCC: CRL-9520 SW 1353 SW 1353 disease: chondrosarcoma 疾病：软骨肉瘤 SW 1353 cell SW 1353 细胞 ATCC: HTB-94 ATCC: HTB-94 LCL-1750 LCL-1750 SW 1990 SW 1990 SW 1990 cell SW 1990 细胞 ATCC: CRL-2172 ATCC: CRL-2172 SW 480 SW 480 SW 480 cell SW 480 细胞 HyperCLDB: cl4441 HyperCLDB: cl4441 SW 620 SW 620 SW 620 cell SW 620 细胞 HyperCLDB: cl4444 HyperCLDB: cl4444 LCL-1718 LCL-1718 SW 780 SW 780 disease: transitional cell carcinoma 疾病：移行细胞癌 SW 780 cell SW 780 细胞 ATCC: CRL-2169 ATCC: CRL-2169 CHEMBL: CHEMBL3308468 CHEMBL: CHEMBL3308468 CVCL: CVCL_1728 CVCL: CVCL_1728 EFO: EFO_0002371 EFO: EFO_0002371 SW 982 SW 982 disease: synovial sarcoma 疾病：滑膜肉瘤 SW 982 cell SW 982 细胞 ATCC: HTB-93 ATCC: HTB-93 SW1116 SW1116 SW1116 cell SW1116 细胞 ATCC: CCL-233 ATCC: CCL-233 SW-13 SW-13 disease: primary small cell carcinoma 疾病：原发性小细胞癌 SW-13 cell SW-13 细胞 ATCC: CCL-105 ATCC: CCL-105 SW48 SW48 disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 SW48 cell SW48 细胞 ATCC: CCL-231 ATCC: CCL-231 SW-480 SW-480 SW-480 cell SW-480 细胞 HyperCLDB: cl5397 HyperCLDB: cl5397 SW480 SW480 disease: colorectal adenocarcinoma 疾病：结肠直肠腺癌 SW480 cell SW480 细胞 ATCC: CCL-228 ATCC: CCL-228 SW579 SW579 disease: squamous cell carcinoma 疾病：鳞状细胞癌 SW579 cell SW579 细胞 ATCC: HTB-107 ATCC: HTB-107 LCL-1157 LCL-1157 SW620 SW620 disease: colorectal adenocarcinoma (colon primary) 疾病：结肠直肠腺癌（结肠原发性） SW620 cell SW620 细胞 ATCC: CCL-227 ATCC: CCL-227 CHEMBL: CHEMBL3307944 CHEMBL: CHEMBL3307944 CVCL: CVCL_0547 CVCL: CVCL_0547 EFO: EFO_0002368 EFO: EFO_0002368 SW626 SW626 SW626 cell SW626 细胞 HyperCLDB: cl4446 HyperCLDB: cl4446 Swiss-3T3 Swiss-3T3 Swiss-3T3 cell Swiss-3T3 细胞 HyperCLDB: cl4451 HyperCLDB: cl4451 MeSH: D041701 MeSH: D041701 T 24 T 24 disease: carcinoma, transitional cell 疾病：癌，移行细胞 T 24 cell T 24 细胞 HyperCLDB: cl4452 HyperCLDB: cl4452 T/G HA-VSMC T/G HA-VSMC T/G HA-VSMC cell T/G HA-VSMC 细胞 ATCC: CRL-1999 ATCC: CRL-1999 LCL-1709 LCL-1709 T24 T24 disease: transitional cell carcinoma 疾病：移行细胞癌 T24 cell T24 细胞 ATCC: HTB-4 ATCC: HTB-4 HyperCLDB: cl5381 HyperCLDB: cl5381 LCL-1486 T-47D T-47D T47D cell T47D细胞 WEB: https://www.atcc.org/products/all/HTB-133.aspx WEB: https://www.atcc.org/products/all/HTB-133.aspx http://bioinformatics.hsanmartino.it/hypercldb/cl4454.html T-47D cell T-47D 细胞 ATCC: HTB-133 ATCC: HTB-133 ECACC: 85102201 HyperCLDB: cl4454 HyperCLDB: cl4454 LCL-1166 LCL-1166 T84 T84 disease: colorectal carcinoma (colon primary) 疾病：结直肠癌（大肠癌） T84 cell T84 细胞 ATCC: CCL-248 ATCC: CCL-248 CHEMBL: CHEMBL3307275 CHEMBL: CHEMBL3307275 CVCL: CVCL_0555 CVCL: CVCL_0555 ECACC: 88021101 ECACC: 88021101 EFO: EFO_0002084 EFO: EFO_0002084 TALL-104 TALL-104 TALL-104 cell TALL-104 细胞 ATCC: CRL-11386 ATCC: CRL-11386 TE671 Subline No.2 TE671 Subline No.2 TE671 Subline No.2 cell TE671 Subline No.2 细胞 HyperCLDB: cl4499 HyperCLDB: cl4499 LCL-1077 LCL-1077 TF-1 TF-1 disease: model for cytokine (IL-3) signaling pathways and myeloid progenitor cell differentiation 疾病：细胞因子（IL-3）信号传导途径和骨髓祖细胞分化的模型 TF-1 cell TF-1 细胞 ATCC: CRL-2003 ATCC: CRL-2003 CHEMBL: CHEMBL3307972 CHEMBL: CHEMBL3307972 CRL-2003 CRL-2003 CVCL: CVCL_0559 CVCL: CVCL_0559 THLE-3 THLE-3 disease: immortalized with SV40 T antigen 疾病：用SV40 T抗原永生化 THLE-3 cell THLE-3 细胞 ATCC: CRL-11233 ATCC: CRL-11233 LCL-1072 LCL-1072 THP-1 THP-1 THP1 THP1 THP-1 cell THP-1 细胞 ATCC: TIB-202 ATCC: TIB-202 CHEMBL: CHEMBL3307574 CHEMBL: CHEMBL3307574 CVCL: CVCL_0006 CVCL: CVCL_0006 EFO: EFO_0001253 EFO: EFO_0001253 HyperCLDB: cl4514 HyperCLDB: cl4514 TM3 TM3 TM3 cell TM3 细胞 ATCC: CRL-1714 ATCC: CRL-1714 TM4 TM4 TM4 cell TM4 细胞 ATCC: CRL-1715 ATCC: CRL-1715 Tn5 B1-4 Tn5 B1-4 Tn5 B1-4 cell Tn5 B1-4 细胞 HyperCLDB: cl4535 HyperCLDB: cl4535 LCL-1690 LCL-1690 TT TT disease: medulla carcinoma 疾病：髓样癌 TT cell TT 细胞 ATCC: COSMIC ID:930299; ATCC CRL-1803,CRL-1803 ATCC: COSMIC ID:930299; ATCC CRL-1803,CRL-1803 ATCC: CRL-1803 ATCC: CRL-1803 CHEMBL: CHEMBL3308145 CHEMBL: CHEMBL3308145 CVCL: CVCL_1774 CVCL: CVCL_1774 EFO: EFO_0002382 EFO: EFO_0002382 U 937 U 937 disease: lymphoma, histiocytic 疾病：淋巴瘤，组织细胞性 U 937 cell U 937 细胞 HyperCLDB: cl4599 HyperCLDB: cl4599 LCL-1358 LCL-1358 U-118 MG U-118 MG U-118 MG cell U-118 MG 细胞 LCL-1431 LCL-1431 U-2 OS U-2 OS U2OS U2OS WEB: https://www.atcc.org/Products/All/HTB-96.aspx WEB: https://www.atcc.org/Products/All/HTB-96.aspx U-2 OS cell U-2 OS 细胞 ATCC: HTB-96 ATCC: HTB-96 CHEMBL: CHEMBL3307757 CHEMBL: CHEMBL3307757 CVCL: CVCL_0042 CVCL: CVCL_0042 EFO: EFO_0002869 EFO: EFO_0002869 LCL-1400 LCL-1400 ZN ZN U-251 MG U-251 MG U-251 MG cell U-251 MG 细胞 HyperCLDB: cl4583 HyperCLDB: cl4583 JHSF: IFO50288 JHSF: IFO50288 LCL-2050 LCL-2050 Oliver He Oliver He U266B1 U266B1 immortal human B cell line cell that was derived from some human B cell isolated from a patient with multiple myeloma 患有多发性骨髓瘤的患者中分离的一些人B细胞衍生的永生人B细胞系细胞 U266B1 cell U266B1 细胞 ATCC: TIB-196 ATCC: TIB-196 ECACC: 85051003 ECACC: 85051003 RRID: CVCL_0566 RRID: CVCL_0566 LCL-1364 LCL-1364 Contaminated/misidentified. This cell line is not the original glioblastoma cell line established in 1968 at the University of Uppsala. As described in PubMed=27582061 it is most probably also a glioblastoma cell line but whose origin is unknow. See U-87MG Uppsala (CVCL_GP63) for the original U-87MG cell line. See more: http://web.expasy.org/cellosaurus/CVCL_0022. 污染/鉴定错误。 该细胞系不是1968年在乌发萨拉大学建立的原始胶质母细胞瘤细胞系。 如PubMed = 27582061中所述，它最可能也是胶质母细胞瘤细胞系，但其起源是未知的。 参见原始U-87MG细胞系的U-87MG Uppsala（CVCL_GP63）。 查看更多：http://web.expasy.org/cellosaurus/CVCL_0022. U-87MG U-87MG U87MG U87MG https://en.wikipedia.org/wiki/U87 https://en.wikipedia.org/wiki/U87 87 MG 87 MG 87MG 87MG U-87 U-87 U87 U87 U87 MG U87 MG U87-MG U87-MG U-87 MG cell U-87 MG 细胞 ATCC: HTB-14 ATCC: HTB-14 CHEMBL: CHEMBL3307575 CHEMBL: CHEMBL3307575 EFO: EFO_0001256 EFO: EFO_0001256 HyperCLDB: cl5280 HyperCLDB: cl5280 RRID:CVCL_0022 RRID:CVCL_0022 LCL-1125 LCL-1125 U-937 U-937 WEB: https://en.wikipedia.org/wiki/U937_cell WEB: https://en.wikipedia.org/wiki/U937_cell a immortal human monocyte cell line cell that was derived from the histiocytic lymphoma of a 37-year-old male patient 源自37岁男性患者的组织细胞性淋巴瘤的永生人单核细胞系细胞 U937 U937 disease: histiocytic lymphoma 疾病：组织细胞性淋巴瘤 U-937 cell U-937 细胞 ATCC: CRL-1593.2 ATCC: CRL-1593.2 CHEMBL: CHEMBL3308006 CHEMBL: CHEMBL3308006 EFO: EFO_0001257 EFO: EFO_0001257 HyperCLDB: cl4605 HyperCLDB: cl4605 MeSH: D020298 MeSH: D020298 RRID: CVCL_0007 RRID: CVCL_0007 LCL-1319 LCL-1319 UACC-812 UACC-812 disease: ductal carcinoma 疾病：导管癌 UACC-812 cell UACC-812 细胞 ATCC: CRL-1897 ATCC: CRL-1897 CHEMBL: CHEMBL3308475 CHEMBL: CHEMBL3308475 CVCL: CVCL_1781 CVCL: CVCL_1781 EFO: EFO_0001258 EFO: EFO_0001258 UMNSAH/DF-1 UMNSAH/DF-1 UMNSAH/DF-1 cell UMNSAH/DF-1 细胞 ATCC: CRL-12203 ATCC: CRL-12203 UMR-106 UMR-106 UMR-106 cell UMR-106 细胞 ATCC: CRL-1661 ATCC: CRL-1661 LCL-1721 LCL-1721 UM-UC-3 UM-UC-3 disease: transitional cell carcinoma 疾病：移行细胞癌 UM-UC-3 cell UM-UC-3 细胞 ATCC: CRL-1749 ATCC: CRL-1749 CHEMBL: CHEMBL3307245 CHEMBL: CHEMBL3307245 CVCL: CVCL_1783 CVCL: CVCL_1783 EFO: EFO_0002387 EFO: EFO_0002387 UT-7 UT-7 disease: leukemia, acute myeloid 疾病：白血病，急性骨髓性白血病 UT-7 cell UT-7 细胞 HyperCLDB: cl5398 HyperCLDB: cl5398 Vero Vero Vero cell Vero 细胞 ATCC: CCL-81 ATCC: CCL-81 HyperCLDB: cl4644 HyperCLDB: cl4644 MeSH: D014709 MeSH: D014709 VERO 76 VERO 76 VERO 76 cell VERO 76 细胞 ATCC: CRL-1587 ATCC: CRL-1587 HyperCLDB: cl4655 HyperCLDB: cl4655 VERO C1008 VERO C1008 VERO C1008 cell VERO C1008 细胞 ATCC: CRL-1586 ATCC: CRL-1586 HyperCLDB: cl4656 HyperCLDB: cl4656 HyperCLDB: cl4657 HyperCLDB: cl4657 Vx2 Vx2 Vx2 cell Vx2 细胞 HyperCLDB: cl4673 HyperCLDB: cl4673 W6/32 W6/32 disease: hybridoma 疾病：杂交瘤 W6/32 cell W6/32 细胞 ATCC: HB-95 ATCC: HB-95 WCH-17 WCH-17 '生物体m'的可能映射 域(值 '土拨鼠，东部') 通过 '东方土拨鼠'的同义词 (NCBI_Taxon: 9995) [SS] Possible mapping of 'Organism' field (value 'woodchuck, Eastern') via synonym of 'Marmota monax' (NCBI_Taxon: 9995) [SS] disease: hepatoma 疾病：肝癌 WCH-17 cell WCH-17 细胞 ATCC: CRL-2082 ATCC: CRL-2082 WEHI 164 WEHI 164 disease: methylcholanthrene-induced fibrosarcoma 疾病：甲基胆蒽诱发的纤维肉瘤 WEHI 164 cell WEHI 164 细胞 ATCC: CRL-1751 ATCC: CRL-1751 WEHI-13VAR WEHI-13VAR disease: fibrosarcoma 疾病：纤维肉瘤 WEHI-13VAR cell WEHI-13VAR 细胞 ATCC: CRL-2148 ATCC: CRL-2148 WEHI-231 WEHI-231 WEHI-231 cell WEHI-231 细胞 ATCC: CRL-1702 ATCC: CRL-1702 WEHI-3 WEHI-3 disease: macrophage like; leukemia 疾病：巨噬细胞样；白血病 WEHI-3 cell WEHI-3 细胞 ATCC: TIB-68 ATCC: TIB-68 WERI-RB-1 WERI-RB-1 WERI-RB-1 cell WERI-RB-1 细胞 HyperCLDB: cl4695 HyperCLDB: cl4695 WI-38 WI-38 WI-38 cell WI-38 细胞 HyperCLDB: cl4703 HyperCLDB: cl4703 WI-38 VA-13 WI-38 VA-13 WI-38 VA-13 cell WI-38 VA-13 细胞 HyperCLDB: cl4711 HyperCLDB: cl4711 WI-38 VA-13 subline 2R WI-38 VA-13 subline 2R WI-38 VA-13 subline 2R cell WI-38 VA-13 subline 2R 细胞 ATCC: CCL-75.1 ATCC: CCL-75.1 WI-38 VA-13 Subline 2RA WI-38 VA-13 Subline 2RA WI-38 VA-13 Subline 2RA cell WI-38 VA-13 Subline 2RA 细胞 HyperCLDB: cl4712 HyperCLDB: cl4712 HyperCLDB: cl4714 HyperCLDB: cl4714 WIL2-S WIL2-S disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 WIL2-S cell WIL2-S 细胞 ATCC: CRL-8885 ATCC: CRL-8885 WISH WISH WISH cell WISH 细胞 ATCC: CCL-25 ATCC: CCL-25 WPMY-1 WPMY-1 disease: adenovirus 12-SV40 hybrid transformed 疾病：腺病毒12-SV40杂交体转化 WPMY-1 cell WPMY-1 细胞 ATCC: CRL-2854 ATCC: CRL-2854 X63AG8.653 X63AG8.653 X63AG8.653 cell X63AG8.653 细胞 HyperCLDB: cl3846 HyperCLDB: cl3846 Y3.AG.1.2.3 Y3.AG.1.2.3 Y3.AG.1.2.3 cell Y3.AG.1.2.3 细胞 HyperCLDB: cl4794 HyperCLDB: cl4794 HyperCLDB: cl4795 HyperCLDB: cl4795 Y3-Ag 1.2.3 Y3-Ag 1.2.3 disease: hybridoma fusion partner 疾病：杂交瘤融合伴侣 Y3-Ag 1.2.3 cell Y3-Ag 1.2.3 细胞 ATCC: CRL-1631 ATCC: CRL-1631 Y79 Y79 Y79 cell Y79 细胞 ATCC: HTB-18 ATCC: HTB-18 HyperCLDB: cl4798 HyperCLDB: cl4798 YAC-1 YAC-1 disease: Mo-MuLV induced lymphoma 疾病：Mo-MuLV诱导的淋巴瘤 YAC-1 cell YAC-1 细胞 ATCC: TIB-160 ATCC: TIB-160 LCL-1321 LCL-1321 ZR-75-1 ZR-75-1 disease: ductal carcinoma (mammary gland epithelium primary) 疾病：导管癌（乳腺上皮细胞原发性） ZR-75-1 cell ZR-75-1 细胞 ATCC: CRL-1500 ATCC: CRL-1500 CHEMBL: CHEMBL3307615 CHEMBL: CHEMBL3307615 CVCL: CVCL_0588 CVCL: CVCL_0588 EFO: EFO_0001262 EFO: EFO_0001262 LCL-1322 LCL-1322 ZR-75-30 ZR-75-30 disease: ductal carcinoma (mammary gland epithelium primary) 疾病：导管癌（乳腺上皮细胞原发性） ZR-75-30 cell ZR-75-30 细胞 ATCC: CRL-1504 ATCC: CRL-1504 CHEMBL: CHEMBL3308094 CHEMBL: CHEMBL3308094 CVCL: CVCL_1661 CVCL: CVCL_1661 EFO: EFO_0001263 EFO: EFO_0001263 ?2 DAP ?2 DAP disease: produces a retrovirus containing human alkaline phosphatase gene 疾病：产生含有人碱性磷酸酶基因的逆转录病毒 ?2 DAP cell ?2 DAP 细胞 ATCC: CRL-1949 ATCC: CRL-1949 An immortal round window-derived cell line cell that derives from human. 来源于人的永生圆窗衍生细胞系细胞。 Yue Liu, Yongqun He immortal human round window-derived cell line cell 永生化的人圆窗衍生细胞系细胞 An immortal bipolar neuron cell line cell that derives from human. 来源于人的永生双极神经元细胞系细胞。 Yue Liu, Yongqun He immortal human bipolar neuron cell line cell 永生化的人双极神经元细胞系细胞 An immortal larva-derived cell line cell that derives from forest day mosquito. 来源于埃及伊蚊的永生幼虫衍生细胞系细胞。 Yue Liu, Yongqun He immortal forest day mosquito larva-derived cell line cell 永生化的白纹伊蚊幼虫衍生细胞系细胞 An immortal peripheral neuron cell line cell that derives from rat. 来源于大鼠的永生外周神经元细胞系细胞。 Yue Liu, Yongqun He immortal rat peripheral neuron cell line cell 永生化的大鼠外周神经元细胞系细胞 An immortal myoblast cell line cell that derives from rat. 来源于大鼠的永生成肌细胞系细胞。 Yue Liu, Yongqun He immortal rat myoblast cell line cell 永生化的大鼠成肌细胞系细胞 An immortal cell line cell that derives from fall armyworm. 来源于秋粘虫的永生细胞系细胞。 Yue Liu, Yongqun He immortal fall armyworm cell line cell 永生化的秋粘虫细胞系细胞 An immortal female gonad-derived cell line cell that derives from fall armyworm. 来源于秋粘虫的永生雌性性腺细胞系细胞。 Yue Liu, Yongqun He immortal fall armyworm female gonad-derived cell line cell 永生化的秋夜蛾雌性性腺衍生细胞系细胞 An immortal adrenal cortex-derived cell line cell that derives from human. 来源于人的永生肾上腺皮质细胞系细胞。 Yue Liu, Yongqun He immortal human adrenal cortex-derived cell line cell 永生化的人肾上腺皮质衍生细胞系细胞 An immortal myeloblast cell line cell that derives from human. 来源于人的永生成髓细胞系细胞。 Yue Liu, Yongqun He immortal human myeloblast cell line cell 永生化的人成髓细胞系细胞 An immortal pair of lungs-derived cell line cell that derives from Mustelinae (i.e. mink). 来源于鼬亚科（即貂）的永生肺对衍生细胞系细胞。 Yongqun He immortal mink cell line cell 永生化的水貂细胞系细胞 LCL-1388 LCL-1388 Oliver He, Jiangan Xie Oliver He, Jiangan Xie SF126 SF126 SF126 cell SF126 细胞 Sanger: COSMIC ID:909712 Sanger: COSMIC ID:909712 LCL-1150 LCL-1150 Oliver He, Jiangan Xie Oliver He, Jiangan Xie HUTU-80 HUTU-80 HUTU-80 cell HUTU-80 细胞 Sanger: COSMIC ID:907073 Sanger: COSMIC ID:907073 LCL-1761 LCL-1761 Oliver He, Jiangan Xie Oliver He, Jiangan Xie A498 A498 A498 cell A498 细胞 Sanger: COSMIC ID:905948 Sanger: COSMIC ID:905948 Chao Pang GM03671 LYMPHOSARCOMA ACUTE LYMPHOCYTIC LEUKEMIA 淋巴细胞急性淋巴细胞白血病 GM03671 cell GM03671 细胞 Chao Pang GM03571 MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS 多发性骨髓瘤（浆细胞瘤）杂交瘤细胞系 GM03571 cell GM03571 细胞 Chao Pang GM03570 MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS 多发性骨髓瘤（浆细胞瘤）杂交瘤细胞系 GM03570 cell GM03570 细胞 Chao Pang GM03569 MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS 多发性骨髓瘤（浆细胞瘤）杂交瘤细胞系 GM03569 cell GM03569 细胞 Chao Pang GM06141 HUMAN ERYTHROLEUKEMIC CELL LINES 人类红细胞系 GM06141 cell GM06141 细胞 Chao Pang GM05887 OSTEOGENIC SARCOMA 成骨肉瘤 GM05887 cell GM05887 细胞 OMIM: 259500 Chao Pang GM15452 CRICETULUS 灰仓鼠 GM15452 cell GM15452 细胞 Chao Pang GM02132 MYELOMA, MULTIPLE AMYLOIDOSIS, PRIMARY, INCLUDED; AL, INCLUDED 包括白血病，多发性淀粉样综合征，原发性; AL，包括在内 GM02132 cell GM02132 细胞 OMIM: 254500 Chao Pang AG07217 WI-38 - SV40 VIRUS-TRANSFORMED FETAL LUNG FIBROBLAST WI-38 - SV40病毒转化的胎儿肺成纤维细胞 AG07217 cell AG07217 细胞 LCL-1873 LCL-1873 Oliver He, Jiangan Xie Oliver He, Jiangan Xie LU-165 LU-165 LU-165 cell LU-165 细胞 Sanger: COSMIC ID:753589 Sanger: COSMIC ID:753589 LCL-1596 LCL-1596 Oliver He, Jiangan Xie Oliver He, Jiangan Xie NCI-H157 NCI-H157 NCI-H157 cell NCI-H157 细胞 Sanger: COSMIC ID:911847 Sanger: COSMIC ID:911847 LCL-1147 LCL-1147 Oliver He, Jiangan Xie Oliver He, Jiangan Xie VCaP VCaP VCaP cell VCaP 细胞 ATCC: CRL-2876 ATCC: CRL-2876 LCL-1499 LCL-1499 Oliver He, Jiangan Xie Oliver He, Jiangan Xie AN3CA AN3CA AN3CA cell AN3CA 细胞 ATCC: HTB-111 ATCC: HTB-111 an immortal T cell line cell that is derived from mouse. 源自小鼠的永生T细胞系细胞。 YH YH immortal mouse T cell line cell 永生化的小鼠T细胞系细胞 An immortal human epithelial cell line cell that derives from human breast. 来源于人乳房的永生人类上皮细胞系细胞。 Yongqun He immortal human breast epithelial cell line cell 永生化的乳腺上皮细胞系细胞 An immortal human epithelial cell line cell that derives from human colon. 来自人类结肠的永生人类上皮细胞系细胞。 Yongqun He immortal human colon epithelial cell line cell 永生化的结肠上皮细胞系细胞 MGI:2159769 MGI:2159769 mouse C57BL/6 inbred strain 小鼠C57BL/6近交系 MGI:2159768 MGI:2159768 mouse DBA/2 inbred strain 小鼠DBA/2近交系 WEB: http://web.expasy.org/cellosaurus/CVCL_J385 WEB: http://web.expasy.org/cellosaurus/CVCL_J385 An immortal mouse T cell line cell derived from a mouse lymphoma, specifically a Rauscher virus-induced tumor.This cell line is antigen processing-defective and expresses a very low level of MHC molecules on its surface. 来源于小鼠淋巴瘤，特别是Rauscher病毒诱导的肿瘤的永生小鼠T细胞系细胞。该细胞系是抗原加工缺陷型，且在其表面表达非常低水平的MHC分子。 RMA cell RMA细胞 OBI: OBI_1110038 OBI: OBI_1110038 RRID: CVCL_J385 RRID: CVCL_J385 An immortal vasculature-derived cell line cell that derives from human. 源自人的永生血系统衍生的细胞系细胞。 Yue Liu, Yongqun He immortal human vasculature-derived endothelial cell line cell' 永生化的血管衍生内皮细胞系细胞 An immortal bone element-derived cell line cell that derives from human. 源自人的永生骨元素衍生细胞系细胞。 Yue Liu, Yongqun He immortal human bone element-derived epithelial cell line cell 永生人骨源性上皮细胞系细胞 An immortal pancreas-derived cell line cell that derives from human. 源自人的永生胰腺衍生细胞系细胞。 Yue Liu, Yongqun He immortal human pancreas-derived epithelial cell line cell 永生化的人胰腺衍生上皮细胞系细胞 An immortal kidney-derived cell line cell that derives from human. 源自人的永生肾衍生细胞系细胞。 Yue Liu, Yongqun He immortal human kidney-derived epithelial cell line cell 永生化的人肾衍生上皮细胞系细胞 Amelogenin: X; CSF1PO: 11,12; D13S317: 12; D16S539: 9; D18S51: 18; D21S11: 28,30.2; D3S1358: 15,17; D5S818: 8,9; D7S820: 11,12; D8S1179: 12,14; FGA: 23; Penta D: 9,10; Penta E: 7,15; TH01: 7,9.3; TPOX: 11; vWA: 16,19 Amelogenin: X; CSF1PO: 11,12; D13S317: 12; D16S539: 9; D18S51: 18; D21S11: 28,30.2; D3S1358: 15,17; D5S818: 8,9; D7S820: 11,12; D8S1179: 12,14; FGA: 23; Penta D: 9,10; Penta E: 7,15; TH01: 7,9.3; TPOX: 11; vWA: 16,19 293 293 293 HEK 293 HEK HEK 293 HEK 293 HEK-293 HEK-293 HEK293 HEK293 WEB: http://hek293.com/ WEB: http://hek293.com/ WEB: http://web.expasy.org/cellosaurus/CVCL_0045 WEB: http://web.expasy.org/cellosaurus/CVCL_0045 WEB: https://www.atcc.org/Products/All/CRL-1573.aspx WEB: https://www.atcc.org/Products/All/CRL-1573.aspx 293衍生细胞 HEK293-derived cell ATCC: CRL-1573 ATCC: CRL-1573 RRID: CVCL_0045 RRID: CVCL_0045 a hybridoma cell line cell that is obtained by fusing activated mouse T cells with tumor cells. 通过将激活的小鼠T细胞与肿瘤细胞融合而获得的杂交瘤细胞系细胞。 Yongqun He Yongqun He https://www.ncbi.nlm.nih.gov/pubmed/18432780 https://www.ncbi.nlm.nih.gov/pubmed/18432780 mouse T-cell hybridoma cell line cell 小鼠T细胞杂交瘤细胞系细胞 https://www.ncbi.nlm.nih.gov/pubmed/2454929 https://www.ncbi.nlm.nih.gov/pubmed/2454929 https://www.ncbi.nlm.nih.gov/pubmed/6181895 https://www.ncbi.nlm.nih.gov/pubmed/6181895 A mouse T-cell hybridoma cell line cell 小鼠T细胞杂交瘤细胞系细胞 Oliver He Oliver He 2B4 cell 2B4细胞 BTO_0005682 BTO_0005682 LCL-1005 BALL1 BALL1 http://web.expasy.org/cellosaurus/CVCL_1075 Ball-1 BALL-1 cell BALL-1细胞 CHEMBL: CHEMBL3308839 RRID:CVCL_1075 RRID:CVCL_1075 non-tumorigenic 无致癌性 Oliver He Oliver He A cell growth process that is conducted in the three dimensional direction. 细胞生长过程是在三维方向进行的。 three dimensional cell growth 三维细胞生长 cell line cell-induced tumor formation 细胞系细胞诱导的肿瘤形成 5637 derived cell line cell 5637衍生细胞系细胞 - - 3111C0001CCC000326 BALB/C小鼠肝瘤株；BNL 1ME A.7R.1 Hepatocellular Carcinoma Cells in BALB/c mice; BNL 1ME A.7R.1 solid tumor 实体瘤 growth in vivo 体内生长 The mouse fetal liver epithelial cells were cultured and treated with methyl cholanthrene and transformed. The transformed cells were transplanted into BALB/C mice in vivo to form solid tumor, which was cryopreserved and can be used to establish the mouse model of human cervical cancer in vivo. 利用培养的小鼠胚胎肝上皮细胞，甲基胆蒽处理，发生了转化。将培养的转化细胞进行BALB/C小鼠体内移植，形成实体瘤，冻存，可用于建立小鼠肝瘤体内移植模型。 - - Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） - - A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Cells were prepared into cell suspension and inoculated into BALB/C mice 制备成细胞悬液接种至BALB/C小鼠 BNL 1ME A.7R.1 BNL 1ME A.7R.1 cell BNL 1ME A.7R.1 细胞 NICR326 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR326 cell NICR326 细胞 http://www.cellresource.cn/fdetail.aspx?id=326 - - 3111C0001CCC000273 BALB/C小鼠肝上皮细胞；BNL 1ME A.7R.1 Hepatocellular Carcinoma Cells in BALB/c mice; BNL 1ME A.7R.1 epithelioid 上皮样 adherent growth 贴壁生长 The cells were established from the transformation of normal liver epithelial cells BNL CL. 2 by the induction of methylcholanthrene. They could grow in 0.3% agar and were negative for ectromelia virus detection. 该细胞由正常肝上皮细胞BNL CL.2经甲基胆蒽诱导转化而建立,在0.3%的琼脂中可生长，鼠痘病毒阴性。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P14 P14 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A BNL 1ME A.7R.1-Species Identification-PUMC BNL 1ME A.7R.1-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:8传代，每周2~3次换液。 Passage at 1: 3 ~ 1: 8, and change fluid for 2 to 3 times per week. BNL 1ME A.7R.1 BNL 1ME A.7R.1 cell BNL 1ME A.7R.1 细胞 NICR273 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR273 cell NICR273 细胞 http://www.cellresource.cn/fdetail.aspx?id=273 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf BNL 1ME A.7R.1-Species Identification-PUMC BNL 1ME A.7R.1-物种鉴定-PUMC - - 3111C0001CCC000194 C3H/An Mouse Connective Tissue Fibroblast Cells; LTK- C3H/An小鼠结缔组织细胞（L929-TK-）；LTK- fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 This cell line is derived from L-M (TK-) and expresses 5HT1D beta receptor. 该细胞派源自于 L-M(TK-)，表达5HT1Dβ受体。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代；每周2次换液。 Passage at 1: 4 ~ 1: 8; and change fluid twice a week. LTK- LTK- cell LTK- 细胞 NICR194 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR194 cell NICR194 细胞 http://www.cellresource.cn/fdetail.aspx?id=194 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf - - 3111C0001CCC000414 C57BL/6 Mouse T-cell Lymphoma Cells; RMA C57BL/6小鼠T细胞淋巴瘤细胞；RMA round 圆形 suspension growth 悬浮生长 RPMI 1640 (w/o Hepes) 10% 小牛血清 RPMI 1640 (w/o Hepes) 10% 小牛血清 RPMI 1640 (w/o Hepes) ?10% calf serum http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com NICR2033 RMA RMA cell RMA 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2033 cell NICR2033 细胞 http://www.cellresource.cn/fdetail.aspx?id=2033 RPMI 1640 (w/o Hepes) 10% 小牛血清 URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% 小牛血清 URI: http://www.cellresource.cn/pdf/20080108101040.pdf - - 3111C0001CCC000317 C57 Mouse Melanoma Tumor Strain; ME （Me） C57小鼠黑色素瘤瘤株；ME （Me） solid tumor, ascites 实体瘤、腹水瘤 growth in vivo 体内生长 Km等小鼠移植，可建立腹水瘤和实体瘤模型。 The model of ascites tumor and solid tumor could be established by transplantation of mice such as Km. - - Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） - - A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Cells were prepared into cell suspension and inoculated into abdominal cavity of Km mice and others. 制备成细胞悬液接种至Km小鼠等腹腔。 ME (Me） ME (Me） cell ME (Me） 细胞 NICR317 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR317 cell NICR317 细胞 http://www.cellresource.cn/fdetail.aspx?id=317 - - 3111C0001CCC000321 C57 Mouse Melanoma Tumor Strain; ME （Me） C57小鼠黑色素瘤瘤株；ME （Me） solid tumor 实体瘤 growth in vivo 体内生长 It is used to a transplanted tumor model for mouse melanoma. 用于建立小鼠黑色素瘤的移植瘤模型。 - - Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） - - A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Cells were prepared into cell suspension and inoculated into C57BL/6 mice. 制备成细胞悬液接种至C57BL/6小鼠。 ME (Me） ME (Me） cell ME (Me） 细胞 NICR321 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR321 cell NICR321 细胞 http://www.cellresource.cn/fdetail.aspx?id=321 STR Identification STR鉴定 3142C0001000000328 EB病毒感染的人外周淋巴细胞；JVM-2 Human Peripheral Lymphocytes Infected by Epstein-Barr Virus; JVM-2 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells were isolated from the peripheral blood of a 63-year-old white woman with mantle cell lymphoma and immortalized by EBV-mediated, expressing p16 and cyclin D2 and low-level of cyclin D1. 该细胞是 J.V.Melo从一位63岁患有套细胞淋巴瘤白人女性外周血中分离建立的，经EBV介导获得永生化，该细胞表达p16和细胞周期蛋白D2,低水平表达细胞周期蛋白D1。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C10 C10 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. JVM-2 JVM-2 cell JVM-2 细胞 NICR1979 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1979 cell NICR1979 细胞 http://www.cellresource.cn/fdetail.aspx?id=1979 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000400003 EB病毒转化的绒猴淋巴细胞；B95-8 Marmoset Lymphocytes Transformed by Epstein-Barr Virus; B95-8 lymphoblast-like 淋巴母细胞样 semi-adherent growth 半贴壁生长 B95-8 is a continuous strain and releases high titer transfected Epstein-Barr virus. This cell line provides Epstein-Barr virus for the establishment of human continuous lymphocyte strains. B95-8细胞株源自暴露于人白血球中抽提的EB病毒的绒猴白血球。 B95-8是一个连续株并释放高滴度的转染EB病毒。 此细胞株提供EB病毒用于建立人的连续淋巴细胞株。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A B95-8-cell morpholgoy-SHCRC B95-8-细胞形态-SHCRC LDH检测报告B95-8-Isoenzyme-SHCRC LDH检测报告B95-8-同工酶-SHCRC MDH检测报告B95-8-Isoenzyme-SHCRC MDH检测报告B95-8-同工酶-SHCRC MPI检测报告B95-8-Isoenzyme-SHCRC MPI检测报告B95-8-同工酶-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. B95-8 B95-8 cell B95-8 细胞 NICR509 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR509 cell NICR509 细胞 http://www.cellresource.cn/fdetail.aspx?id=509 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf B95-8-cell morpholgoy-SHCRC B95-8-细胞形态-SHCRC LDH检测报告B95-8-Isoenzyme-SHCRC LDH检测报告B95-8-同工酶-SHCRC MDH检测报告B95-8-Isoenzyme-SHCRC MDH检测报告B95-8-同工酶-SHCRC MPI检测报告B95-8-Isoenzyme-SHCRC MPI检测报告B95-8-同工酶-SHCRC 3142C0001000000015 EB病毒转化的绒猴淋巴细胞；B95-8 Marmoset Lymphocytes Transformed by Epstein-Barr Virus; B95-8 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 B95-8 cell line is derived from marmosets white blood cell. B95-8 is a continuous cell strain and released high titer Epstein-Barr virus. This cell line provides Epstein-Barr virus for the establishment of human continuous lymphocyte strains. B95-8细胞株源自绒猴白细胞。B95-8是连续传代株，并释放高滴度的EB病毒。 此细胞株提供EB病毒用于建立人的连续淋巴细胞株。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C12 C12 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. B95-8 B95-8 cell B95-8 细胞 NICR783 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR783 cell NICR783 细胞 http://www.cellresource.cn/fdetail.aspx?id=783 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf - - 3153C0001000000039 EB病毒转化的绒猴淋巴细胞；B95-8 Marmoset Lymphocytes Transformed by Epstein-Barr Virus; B95-8 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This cell line is derived from monkey white blood cells infected with Epstein-Barr virus. B95-8 is a continuous strain and releases high titer Epstein-Barr virus. This cell line can provide Epstein-Barr virus for the establishment of human continuous lymphocyte strains. 该细胞系来源于感染了EB病毒的绒猴白血球。B95-8是一个连续株并释放高滴度的EB病毒。 此细胞株可提供EB病毒用于建立人的连续淋巴细胞株。 RPMI 1640 (w/o Hepes) 15%NBS RPMI 1640 (w/o Hepes) 15%NBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN12 PN12 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） 44-47 44-47 A类 Class A B95-8-cell morphology-KCB B95-8-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1:2传代；5-6天一次 Passage at 1: 2; and passage once in 5-6 days B95-8 B95-8 cell B95-8 细胞 NICR1313 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1313 cell NICR1313 细胞 http://www.cellresource.cn/fdetail.aspx?id=1313 RPMI 1640 (w/o Hepes) 15%NBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 15%NBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf B95-8-cell morphology-KCB B95-8-细胞形态-KCB Amelogenin：X；CSF1PO：11，12；D13S317：12；D16S539：9，14；D18S51：17，18；D19S433：17，18；D21S11：28，30.2；D2S1338：19；D3S1358：5，16，17；D5S818：8，9；D7S820：11，12；D8S1179：12，13，14；FGA：21，22，23；TH01：7，9.3；TPOX：11；vWA：16，19，20； Amelogenin：X；CSF1PO：11，12；D13S317：12；D16S539：9，14；D18S51：17，18；D19S433：17，18；D21S11：28，30.2；D2S1338：19；D3S1358：5，16，17；D5S818：8，9；D7S820：11，12；D8S1179：12，13，14；FGA：21，22，23；TH01：7，9.3；TPOX：11；vWA：16，19，20； 3111C0001CCC000091 Human Embryonic Kidney Cells Transformed with SV40 Large T-Antigen; 293T SV40T转化的人胚肾细胞；293T epithelioid 上皮样 adherent growth 贴壁生长 The cells express SV40 large T antigen, which can be used for transfection and used as packaging cells, and the cells express high dose of AP fusion protein when used for transient transfection. 表达SV40大T抗原，可用于转染和作为包装细胞；用于瞬时转染时可高表达AP融合蛋白。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 62~69 62~69 A类 Class A 293T-Species Identification-PUMC 293T-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~8传代；26~36小时1次 Passage at 1: 4 ~ 8; and passage once for 26 ~ 36 hours 293T 293T cell 293T 细胞 NICR91 PMID: 10559361 PMID: 10559361 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR91 cell NICR91 细胞 http://www.cellresource.cn/fdetail.aspx?id=91 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf 293T-Species Identification-PUMC 293T-物种鉴定-PUMC Amelogenin:X；CSF1PO:11,12；D13S317:12,14；D16S539:9,13；D18S51:17,18；D19S433:18；D21S11:28,30.2；D2S1338:19；D3S1358:15,17；D5S818:8,9；D7S820:11；D8S1179:12,14；FGA:23；TH01:7,9.3；TPOX:11；vWA:16,19 Amelogenin:X；CSF1PO:11,12；D13S317:12,14；D16S539:9,13；D18S51:17,18；D19S433:18；D21S11:28,30.2；D2S1338:19；D3S1358:15,17；D5S818:8,9；D7S820:11；D8S1179:12,14；FGA:23；TH01:7,9.3；TPOX:11；vWA:16,19 3131C0001000200017 Human Embryonic Kidney Cells Transformed with SV40 Large T-Antigen; 293T SV40T转化的人胚肾细胞；293T epithelial cell 上皮细胞 adherent growth 贴壁生长 293细胞株插入SV40 T-抗原的温度敏感基因后产生的高转染效率的衍生株称为293T。 A derived cell line of the high transfection efficiency produced after insertion of the temperature sensitive gene of SV40 T antigen in 293 cell lines is called 293T. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A 293T-cell morphology-SHCRC 293T-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 293T 293T cell 293T 细胞 NICR476 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR476 cell NICR476 细胞 http://www.cellresource.cn/fdetail.aspx?id=476 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf 293T-cell morphology-SHCRC 293T-细胞形态-SHCRC Amelogenin：X；CSF1PO：11，12；D13S317：12；D16S539：9，13；D18S51：17，18；D19S433：17，18；D21S11：28，30.2；D2S1338：19；D3S1358：15，16，17；D5S818：8，9；D7S820：12；D8S1179：11，12，14；FGA：23；TH01：7，9.3；TPOX：11；vWA：16，19； Amelogenin：X；CSF1PO：11，12；D13S317：12；D16S539：9，13；D18S51：17，18；D19S433：17，18；D21S11：28，30.2；D2S1338：19；D3S1358：15，16，17；D5S818：8，9；D7S820：12；D8S1179：11，12，14；FGA：23；TH01：7，9.3；TPOX：11；vWA：16，19； 3111C0001CCC000212 Human Embryonic Kidney Cells Transformed with SV40 Large T-Antigen(subline); 293T/17 SV40T转化的人胚肾细胞（亚系）；293T/17 epithelioid 上皮样 adherent growth 贴壁生长 This cell is derived from 293T cells expressing SV40 T antigen; it can produce a high titer of infectious retroviruses. 该细胞来源于表达SV40T抗原的293T细胞；可以产生高滴度的有感染性的逆转录病毒。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A 293T-17-Species Identification-PUMC 293T-17-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代；2~3天换液1次。 Passage at 1: 4 ~ 1: 8; and change fluid once in 2~3 days. 293T/17 293T/17 cell 293T/17 细胞 NICR212 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR212 cell NICR212 细胞 http://www.cellresource.cn/fdetail.aspx?id=212 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100710.pdf 293T-17-Species Identification-PUMC 293T-17-物种鉴定-PUMC - - 3111C0001CCC000157 SV40-transformed Green Monkey Kidney Cells; COS-1 SV40转化的非洲绿猴肾细胞；COS-1 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 This cell is derived from a CV-1 cell line, obtained by transfection of SV40 encoding a wild-type T antigen with a starting point defect mutation; a single copy of the complete early segment of the SV40 genome is integrated. The cells express T antigen, suitable for transfection of vectors requiring expression of SV40 T antigen; the cells retain the properties of SV40 lytic growth and support replication of temperature sensitive A209 virus at 40 ℃, and support the transfection of SV40 pure body at early segment deletion. Because of the DNA sequence containing SV40, the cells need to be operated in a class 2 biosafety cabinet. 该细胞源自CV-1细胞株，经转染编码野生型T抗原、起始点缺陷突变的SV40得到；细胞中整合有SV40基因组完整早期区段的单个拷贝。该细胞表达T抗原，适用于需要SV40T抗原表达的载体的转染；保留SV40溶细胞性生长的特性；支持40℃时温度敏感性A209病毒的复制；支持早期区段缺失的SV40纯体的复制。因含有SV40的DNA序列，该细胞需要在2级生物安全柜中操作。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C6 C6 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 48~103 48~103 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代；2~3天换液1次。　 Passage at 1: 4 ~ 1: 8; and change fluid once in 2~3 days. COS-1 COS-1 cell COS-1 细胞 NICR157 PMID: 8643633 PMID: 8643633 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR157 cell NICR157 细胞 http://www.cellresource.cn/fdetail.aspx?id=157 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3131C0001000400001 SV40-transformed Green Monkey Kidney Cells; COS-1 SV40转化的非洲绿猴肾细胞；COS-1 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 This cell is derived from a CV-1 cell line, obtained by transfection of SV40 encoding a wild-type T antigen with a starting point defect mutation; a single copy of the complete early segment of the SV40 genome is integrated. The cells express T antigen, suitable for transfection of vectors requiring expression of SV40 T antigen; the cells retain the properties of SV40 lytic growth and support replication of temperature sensitive A209 virus at 40 ℃, and support the transfection of SV40 pure body at early segment deletion. Because of the DNA sequence containing SV40, the cells need to be operated in a class 2 biosafety cabinet. 该细胞源自CV-1细胞株，经转染编码野生型T抗原、起始点缺陷突变的SV40得到；细胞中整合有SV40基因组完整早期区段的单个拷贝。该细胞表达T抗原，适用于需要SV40T抗原表达的载体的转染；保留SV40溶细胞性生长的特性；支持40℃时温度敏感性A209病毒的复制；支持早期区段缺失的SV40纯体的复制。因含有SV40的DNA序列，该细胞需要在2级生物安全柜中操作。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1：3传代；3-4天一次 Passage at 1: 3; and passage once in 3-4 days COS-1 COS-1 cell COS-1 细胞 NICR507 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR507 cell NICR507 细胞 http://www.cellresource.cn/fdetail.aspx?id=507 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf - - 3153C0001000000088 SV40-transformed Green Monkey Kidney Cells; COS-1 SV40转化的非洲绿猴肾细胞；COS-1 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 This cell is derived from a CV-1 cell line, obtained by transfection of SV40 encoding a wild-type T antigen with a starting point defect mutation; a single copy of the complete early segment of the SV40 genome is integrated. The cells express T antigen, suitable for transfection of vectors requiring expression of SV40 T antigen; the cells retain the properties of SV40 lytic growth and support replication of temperature sensitive A209 virus at 40 ℃, and support the transfection of SV40 pure body at early segment deletion. Because of the DNA sequence containing SV40, the cells need to be operated in a class 2 biosafety cabinet. 该细胞源自CV-1细胞株，经转染编码野生型T抗原、起始点缺陷突变的SV40得到；细胞中整合有SV40基因组完整早期区段的单个拷贝。该细胞表达T抗原，适用于需要SV40T抗原表达的载体的转染；保留SV40溶细胞性生长的特性；支持40℃时温度敏感性A209病毒的复制；支持早期区段缺失的SV40纯体的复制。因含有SV40的DNA序列，该细胞需要在2级生物安全柜中操作。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN2 PN2 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） 99-110 99-110 A类 Class A COS-1-cell morphology-KCB COS-1-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1：3传代；3-4天一次 Passage at 1: 3; and passage once in 3-4 days COS-1 COS-1 cell COS-1 细胞 NICR1362 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1362 cell NICR1362 细胞 http://www.cellresource.cn/fdetail.aspx?id=1362 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf COS-1-cell morphology-KCB COS-1-细胞形态-KCB - - 3111C0001CCC000033 SV40-transformed Green Monkey Kidney Cells; COS-7 SV40转化的非洲绿猴肾细胞；COS-7 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 This cell line is derived from CV-1 cell line and is obtained by transfected with SV40 virus mutant with deletion of starting point; it encodes for expression of wild-type T antigen, so the cell is suitable as a transfection host for vector requiring expression of SV40 T antigen. The cells express T antigen, allowing soluble growth of SV40 virus, supporting replication of temperature-sensitive A209 virus at 40℃, and supporting replication of SV40 mutants with starting region defects. Because of the DNA sequence containing SV40 virus, the cells need to be operated in class II biological safety cabinets. 此细胞株源自CV-1细胞株，经转染起始点缺失的SV40病毒突变体得到；编码表达野生型T抗原，所以该细胞适合作为需要SV40 T抗原表达的载体的转染宿主。该细胞表达T抗原，允许SV40病毒的溶解性生长，支持40℃时温度敏感性A209病毒的复制，支持起始区域缺陷的SV40突变体的复制。因含有SV40病毒的DNA序列，该细胞需要在2级生物安全柜中操作。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 83~102 83~102 A类 Class A COS-7-Species Identification-PUMC COS-7-cell morphology-PUMC COS-7-物种鉴定-PUMC COS-7-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代；每周2~3次。　 Passage at 1: 4 ~ 1: 8; and passage for 2 to 3 times per week. COS-7 COS-7 cell COS-7 细胞 NICR33 PMID: 8631990 PMID: 8631990 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR33 cell NICR33 细胞 http://www.cellresource.cn/fdetail.aspx?id=33 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf COS-7-Species Identification-PUMC COS-7-cell morphology-PUMC COS-7-物种鉴定-PUMC COS-7-细胞形态-PUMC 3131C0001000400002 SV40-transformed Green Monkey Kidney Cells; COS-7 SV40转化的非洲绿猴肾细胞；COS-7 fibroblasts 成纤维细胞 adherent growth 贴壁生长 This cell line is derived from a CV-1 cell line and is transfected with SV40 with an initial inactivation mutation encoding the wild-type T antigen. 此细胞株源自CV-1细胞株，经带有编码野生型T抗原的起始失活突变的SV40转染得到。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A COS-7-cell morphology-SHCRC COS-7-细胞形态-SHCRC LDH检测报告COS-7-Isoenzyme-SHCRC LDH检测报告COS-7-同工酶-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 COS-7 COS-7 cell COS-7 细胞 NICR508 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR508 cell NICR508 细胞 http://www.cellresource.cn/fdetail.aspx?id=508 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf COS-7-cell morphology-SHCRC COS-7-细胞形态-SHCRC LDH检测报告COS-7-Isoenzyme-SHCRC LDH检测报告COS-7-同工酶-SHCRC STR Identification STR鉴定 3142C0001000000088 SV40-transformed Green Monkey Kidney Cells; COS-7 SV40转化的非洲绿猴肾细胞；COS-7 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line is derived from CV-1 cell line and is obtained by transfected with SV40 virus mutant with deletion of starting point; it encodes for expression of wild-type T antigen, so the cell is suitable as a transfection host for vector requiring expression of SV40 T antigen. The cells express T antigen, allowing soluble growth of SV40 virus, supporting replication of temperature-sensitive A209 virus at 40℃, and supporting replication of SV40 mutants with starting region defects. Because of the DNA sequence containing SV40 virus, the cells need to be operated in class II biological safety cabinets. 此细胞株源自CV-1细胞株，经转染起始点缺失的SV40病毒突变体得到；编码表达野生型T抗原，所以该细胞适合作为需要SV40 T抗原表达的载体的转染宿主。该细胞表达T抗原，允许SV40病毒的溶解性生长，支持40℃时温度敏感性A209病毒的复制，支持起始区域缺陷的SV40突变体的复制。因含有SV40病毒的DNA序列，该细胞需要在2级生物安全柜中操作。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C78 C78 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. COS-7 COS-7 cell COS-7 细胞 NICR856 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR856 cell NICR856 细胞 http://www.cellresource.cn/fdetail.aspx?id=856 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf - - 3153C0001000000037 SV40-transformed Green Monkey Kidney Cells; COS-7 SV40转化的非洲绿猴肾细胞；COS-7 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 This cell line is derived from CV-1 cell line and is obtained by tramsfection with SV40 virus mutant with deletion at the starting point; it encodes the expression of wild-type T antigen, so the cell is suitable as a transfection host for vector requiring SV40 T antigen expression. The cells express T antigen, allowing soluble growth of SV40 virus, supporting replication of temperature-sensitive A209 virus at 40℃, and supporting replication of SV40 mutants with starting region defects. Because of the DNA sequence containing SV40 virus, the cells need to be operated in a class 2 biosafety cabinet. 该细胞株源自CV-1细胞株，经转染起始点缺失的SV40病毒突变体得到；编码表达野生型T抗原，所以该细胞适合作为需要SV40 T抗原表达的载体的转染宿主。该细胞表达T抗原，允许SV40病毒的溶解性生长，支持40℃时温度敏感性A209病毒的复制，支持起始区域缺陷的SV40突变体的复制。因含有SV40病毒的DNA序列，该细胞需要在2级生物安全柜中操作。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN2 PN2 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） 51-129 51-129 A类 Class A COS-7-cell morphology-KCB COS-7-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1：3传代；3-4天一次 Passage at 1: 3; and passage once in 3-4 days COS-7 COS-7 cell COS-7 细胞 NICR1311 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1311 cell NICR1311 细胞 http://www.cellresource.cn/fdetail.aspx?id=1311 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf COS-7-cell morphology-KCB COS-7-细胞形态-KCB 3131C0001000200014 Human SV40 Transfected Osteoblasts; hFOB 1.19 SV40转染人成骨细胞；hFOB 1.19 adherent growth 贴壁生长 This cell line was established by transfection of fetal limb tissue of spontaneous abortionin with temperature-sensitive expression vector pUCSVisA58 in the presence of 0.6 mg/ml neomycin G418. Cell division was rapid at the permissible temperature of 33.5℃, while at the limit temperature of 39.5℃, the cells were little or no division. These cells have the ability to differentiate into mature osteoclasts that express the osteoblast phenotype. These cells provide a homogeneous rapid proliferation model system for studying the differentiation of normal human osteoblasts, the physiology and hormones of osteoblasts, growth factors, and cytokines that affect the function and differentiation of osteoblasts. 在0.6mg/ml新霉素G418存在下，用温度敏感的表达载体pUCSVisA58转染自然流产的胎儿四肢组织，建立了此细胞系。 在许可温度33.5oC下细胞分裂很快，而在限制温度39.5oC下细胞极少分裂或不分裂。 这些细胞具有分化为表达造骨细胞表型的成熟造骨细胞的能力。 这些细胞提供了一个同质化的快速增殖模型系统，用于研究正常人造骨细胞的分化，造骨细胞生理和激素，生长因子，和对造骨细胞的功能及分化有影响的细胞因子。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) qualified fetal bovine serum，10%；0.3mg/ml G418 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10%；0.3mg/ml G418 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10%；0.3mg/ml G418 http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A hFOB 1 19-cell morphology-SHCRC hFOB 1 19-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR473 hFOB 1.19 hFOB 1.19 cell hFOB 1.19 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR473 cell NICR473 细胞 http://www.cellresource.cn/fdetail.aspx?id=473 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10%；0.3mg/ml G418 URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10%；0.3mg/ml G418 URI: http://www.cellresource.cn/pdf/20080108100542.pdf hFOB 1 19-cell morphology-SHCRC hFOB 1 19-细胞形态-SHCRC Amelogenin：X；CSF1PO:10,13；D12S391:20,23；D13S317:11,12；D16S539:9,13；D18S51:10,17；D19S433:13,15；D21S11:29,32.2；D2S1338:23,24；D3S1358:17,18；D5S818:11,12；D6S1043:13,16；D7S820:8,10；D8S1179:10,14；FGA:19,22；Penta E:8,11；TH01:7,9.3；TPOX:11；vWA:16,18； Amelogenin：X；CSF1PO:10,13；D12S391:20,23；D13S317:11,12；D16S539:9,13；D18S51:10,17；D19S433:13,15；D21S11:29,32.2；D2S1338:23,24；D3S1358:17,18；D5S818:11,12；D6S1043:13,16；D7S820:8,10；D8S1179:10,14；FGA:19,22；Penta E:8,11；TH01:7,9.3；TPOX:11；vWA:16,18； 3111C0001CCC000487 Human SV40 Transfected Osteoblasts; hFOB 1.19 SV40转染人成骨细胞；hFOB 1.19 polygonal 多角 adherent growth 贴壁生长 This cell line was established by transfection of fetal limb tissue of spontaneous abortionin with temperature-sensitive expression vector pUCSVisA58 in the presence of 0.6 mg/ml neomycin G418. Cell division was rapid at the permissible temperature of 33.5℃, while at the limit temperature of 39.5℃, the cells were little or no division. These cells have the ability to differentiate into mature osteoclasts that express the osteoblast phenotype. These cells provide a homogeneous rapid proliferation model system for studying the differentiation of normal human osteoblasts, the physiology and hormones of osteoblasts, growth factors, and cytokines that affect the function and differentiation of osteoblasts. 在0.6mg/ml新霉素G418存在下，用温度敏感的表达载体pUCSVisA58转染自然流产的胎儿四肢组织，建立了此细胞系。 在许可温度33.5oC下细胞分裂很快，而在限制温度39.5oC下细胞极少分裂或不分裂。 这些细胞具有分化为表达造骨细胞表型的成熟造骨细胞的能力。 这些细胞提供了一个同质化的快速增殖模型系统，用于研究正常人造骨细胞的分化，造骨细胞生理和激素，生长因子，和对造骨细胞的功能及分化有影响的细胞因子。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代，3天传代一次 Passage at 1: 2, and passage once in 3 days NICR2160 hFOB 1.19 hFOB 1.19 cell hFOB 1.19 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2160 cell NICR2160 细胞 http://www.cellresource.cn/fdetail.aspx?id=2160 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) URI: http://www.cellresource.cn/pdf/20080108100542.pdf - - 3111C0001CCC000316 Walker 256 Carcinosarcoma; W256 Walker氏癌肉瘤256瘤株；W256 solid tumor 实体瘤 growth in vivo 体内生长 The cell line is a common rat-derived tumor strain and used to establish rat tumor transplantation models. 常用的大鼠来源瘤株，用于建立大鼠肿瘤移植模型。 - - Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） - - A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Cells were prepared into cell suspension and inoculated into Wistar rats. 制备成细胞悬液接种至Wistar大鼠。 NICR316 W256 W256 cell W256 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR316 cell NICR316 细胞 http://www.cellresource.cn/fdetail.aspx?id=316 3131C0001001100002 Zebrafish Caudal Fin Cells; AB.9 斑马鱼尾鳍细胞；AB.9 adherent growth 贴壁生长 39 39 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn AB.9 AB.9 cell AB.9 细胞 NICR765 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR765 cell NICR765 细胞 http://www.cellresource.cn/fdetail.aspx?id=765 - - 3111C0001CCC000211 Hamster-mouse Hybridoma Cells; 145-2C11 仓鼠/小鼠杂交瘤细胞；145-2C11 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 It is a hybridoma cell. American hamster were immunized with BM10-37 mouse CTL cell clone (anti-H-2 Kb), then spleen cells were fused with Sp2/0-Ag14 myeloma cells; the cells are anti-mouse CD3 and IgG type. 杂交瘤细胞，用BM10-37小鼠CTL细胞克隆（抗H-2 Kb）免疫美国仓鼠，取脾细胞与Sp2/0-Ag14 骨髓瘤细胞融合得到；抗小鼠CD3，IgG型。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS；4mM L-glutamine IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS；4mM L-glutamine http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 0.1 ~ 1 � 106 cells/ml; and change fluid once in 2~3 days. 维持细胞浓度在0.1~1×106 cells/ml；2~3天换液1次。 145-2C11 145-2C11 cell 145-2C11 细胞 NICR211 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR211 cell NICR211 细胞 http://www.cellresource.cn/fdetail.aspx?id=211 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS；4mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS；4mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100822.pdf 3131C0001000100008 Hamster Ovary Cells; Lec1 仓鼠卵巢细胞；Lec1 epithelial cell 上皮细胞 adherent growth 贴壁生长 Lec1 (formerly called Pro-5 wgaR1 3C) is a mutant strain capable of resisting wheat germ agglutinin selected from the proline-deficient CHO clone Pro-5. Lec1 is called a glycosyltransferase of GlcNAc-T1, so that N-linked carbohydrates are blocked in Man5-GlcNAC2-Asn intermediate. These cells are very useful for studying the effect of N-linked glycosylation changes on the function and segregation of endogenous glycoproteins or viral infections, glycoproteins produced by transfection with cloned DNA. Lec1 (原先叫做Pro-5wgaR1 3C)是从脯氨酸缺陷型的CHO克隆 Pro-5中挑选出来的能抗麦芽凝集素的突变株。 Lec1 缺乏称作GlcNAc-T1的糖基转移酶，从而N-连接碳水化合物被阻断在Man5-GlcNAC2-Asn中间体。 对于研究N-连接糖基化改变对内源糖蛋白或病毒感染、以克隆DNA转染产生的糖蛋白的功能和区隔化的影响，这些细胞十分有用。 http://www.cellresource.cn/pdf/20080108100503.pdf http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) qualified fetal bovine serum，10% α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 Lec1 Lec1 cell Lec1 细胞 NICR460 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR460 cell NICR460 细胞 http://www.cellresource.cn/fdetail.aspx?id=460 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100503.pdf 3111C0002000000107 Ovarian Cells of Spodoptera Fruigiperda; Sf9 草地夜蛾卵巢细胞；Sf9 epithelioid 上皮样 semi-adherent 半贴壁 This cell is sensitive to spodoptera exigua, baculovirus, and can be used for replication of baculovirus. 该细胞对丫纹夜蛾、杆状病毒敏感，可用于杆状病毒的复制。 10%FBS 10%FBS 90%FBS+10%DMSO 90%FBS+10%DMSO A类 Class A Sf9-cell morphology-NIFDC Sf9-细胞形态-NIFDC National Institute for Food and Drug Control 中国药品生物制品检定所 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:5 or greater Passage at 1: 5 or greater NICR2178 Sf9 Sf9 cell Sf9 细胞 NICR2178 cell NICR2178 细胞 http://www.cellresource.cn/fdetail.aspx?id=2178 Sf9-cell morphology-NIFDC Sf9-细胞形态-NIFDC - - 3111C0001CCC000008 Rat Pituitary Tumor Cells; GH3 大鼠垂体瘤细胞；GH3 epithelioid 上皮样 loosely adherent, floating clusters of cells 疏松贴壁，有漂浮的细胞簇 GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆，而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素，也可产生催乳素。对调控GH3细胞分泌蛋白类激素的研究表明，氢化可的松可以刺激生长激素的分泌、抑制催乳素的产生。 The GH3 cell line was isolated from pituitary tumor of a 7-month-old female Wistar-Furth rat in July 1965 by Tashjian AH et al. The GH3 cell line was not directly derived from clones of the GH1 cell line, but was established from tumors formed by two passages of primarily cultured GH1 cells in rats. Epithelial-like GH3 cells secrete higher levels of growth hormone than GH1 and also produce prolactin. The research of regulating GH3 cell secretion of protein-like hormone showed that hydrocortisone can stimulate the secretion of growth hormone and inhibit the production of prolactin. F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS http://www.cellresource.cn/pdf/20080108100736.pdf http://www.cellresource.cn/pdf/20080108100736.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 62~68 62~68 A类 Class A GH3-cell morphology-PUMC GH3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；每周2~3次。 Passage at 1: 2 ~ 1: 4; and passage for 2 to 3 times per week. GH3 GH3 cell GH3 细胞 NICR8 PMID: 5513559 PMID: 5513559 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR8 cell NICR8 细胞 http://www.cellresource.cn/fdetail.aspx?id=8 F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS URI: http://www.cellresource.cn/pdf/20080108100736.pdf F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS URI: http://www.cellresource.cn/pdf/20080108100736.pdf GH3-cell morphology-PUMC GH3-细胞形态-PUMC - - 3111C0001CCC000081 Rat Pituitary Tumor Cells; MMQ 大鼠垂体瘤细胞；MMQ epithelioid 上皮样 suspension growth 悬浮生长 The cells are derived from rat pituitary adenomas and secrete prolactin and express functional dopamine receptors. The cells do not form tumors in immunosuppressed mice, but can form clones in semi-solid medium. 该细胞源自大鼠垂体瘤，分泌泌乳素；表达功能性多巴胺受体。该细胞在免疫抑制的小鼠中不能成瘤，但在半固体培养基中可形成克隆。 F12: Ham＇s F12 Nutrient Mixture 2.5%FBS+15%HS F12: Ham＇s F12 Nutrient Mixture 2.5%FBS+15%HS http://www.cellresource.cn/pdf/20080108100810.pdf http://www.cellresource.cn/pdf/20080108100810.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 47~52 47~52 A类 Class A MMQ-Species Identification-PUMC MMQ-cell morphology-PUMC MMQ-物种鉴定-PUMC MMQ-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 1 � 105 ~ 1 � 106 cells/ml; 维持细胞浓度在1×105~1×106 cells/ml；2天换液1次。 MMQ MMQ cell MMQ 细胞 NICR81 PMID: 12208657 PMID: 12208657 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR81 cell NICR81 细胞 http://www.cellresource.cn/fdetail.aspx?id=81 F12: Ham＇s F12 Nutrient Mixture 2.5%FBS+15%HS URI: http://www.cellresource.cn/pdf/20080108100810.pdf F12: Ham＇s F12 Nutrient Mixture 2.5%FBS+15%HS URI: http://www.cellresource.cn/pdf/20080108100810.pdf MMQ-Species Identification-PUMC MMQ-cell morphology-PUMC MMQ-物种鉴定-PUMC MMQ-细胞形态-PUMC 3131C0001000500009 Rat Alveolar Macrophages; NR8383[AgC11x3A; NR8383.1] 大鼠肺泡巨噬细胞；NR8383[AgC11x3A; NR8383.1] megakaryocyte 巨噬细胞 semi-adherent growth 半贴壁生长 NR8383 (normal rats, on Aug. 3, 1983) was derived from normal rat alveolar macrophages during lung lavage. The cells were cultured for approximately 8-9 months in the presence of gerbil lung cell continuous culture medium. Subsequently, exogenous growth factors are no longer needed. NR8383 cells were cloned and subcloned from single cells by limited dilution and subcloned three times with soft agar. The cells were characterized by macrophage characteristics, phagocytosis of yeast polysaccharide and cupric carbonate basic, non-specific lipase activity, Fc receptor, and oxidative degradation; The cells express IL-1,TNF beta and IL-6 secretion, and response repeately to exogenous growth factors. NR8383 cells respond to bleomycin and secrete TGF beta precursors. NR8383 (正常大鼠，1983年8月3日)来源于肺灌洗时的正常大鼠肺泡巨噬细胞。 细胞在gerbil肺细胞连续培养液存在下培养了大约8-9个月。 随后，不再需要外源生长因子。 通过有限稀释法从单个细胞克隆并亚克隆NR8383细胞，并三次用软琼脂亚克隆。 细胞表现出巨噬细胞的特性，吞噬酵母多糖和铜绿，非特异性脂酶活性，Fc受体，氧化降解；分泌IL-1, TNF beta和IL-6，可重复地响应外源生长因子。 NR8383细胞响应博莱霉素，分泌TGF beta前体。 F12K qualified fetal bovine serum，20% F12K 优质胎牛血清，20% F12K 优质胎牛血清，20% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. AgC11x3A AgC11x3A cell AgC11x3A 细胞 NICR538 NR8383 NR8383 cell NR8383 细胞 NR8383.1 NR8383.1 cell NR8383.1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR538 cell NICR538 细胞 http://www.cellresource.cn/fdetail.aspx?id=538 F12K 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108102725.pdf STR Identification STR鉴定 3142C0001000000059 Rat Ascites Tumor Cells; Walker/LLC-WRC 大鼠腹水癌细胞；Walker/LLC-WRC lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 Walker 256 is a rat ascites cancer cell line. The cells injected into the abdominal cavity of rats can rapidly form ascites tumor, which is an excellent material for studying the model of antineoplastic drugs in vivo. Walker 256是一株大鼠腹水癌细胞系。该细胞注入大鼠腹腔能快速形成腹水瘤，是研究抗肿瘤药物体内模型的极好材料。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C180 C180 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR827 Walker/LLC-WRC Walker/LLC-WRC cell Walker/LLC-WRC 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR827 cell NICR827 细胞 http://www.cellresource.cn/fdetail.aspx?id=827 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001001000004 Rat Hepatoma Cells; RH-35 大鼠肝癌细胞；RH-35 epithelial-like 上皮细胞样 adherent growth 贴壁生长 RH-35细胞株源自由N-2feuorenyldiuctamid在雄性AxC大鼠中诱导的可移植Reulier H-35肝癌。 细胞较小，提供者称饥饿24小时可以使其同步。 Reulier H-35 cell line is derived from transplantable Reulier H-35 hepatoma induced by N-2 feurenyldiuctamid in male AxC rats. The cells are smaller, and the provider said that starvation for 24 hours can make the cells synchronized. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR761 RH-35 RH-35 cell RH-35 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR761 cell NICR761 细胞 http://www.cellresource.cn/fdetail.aspx?id=761 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3131C0001000500001 Rat Hepatocytes; BRL 大鼠肝细胞；BRL epithelial-like 上皮细胞样 adherent growth 贴壁生长 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A BRL-cell morpholgoy-SHCRC BRL-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 3. The cells can be overgrowth within 3 days. 消化3-5分钟。1:3。3天内可长满。 BRL BRL cell BRL 细胞 NICR530 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR530 cell NICR530 细胞 http://www.cellresource.cn/fdetail.aspx?id=530 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf BRL-cell morpholgoy-SHCRC BRL-细胞形态-SHCRC 3131C0001000500003 Rat Hepatocytes; BRL 3A 大鼠肝细胞；BRL 3A adherent growth 贴壁生长 The serum-free conditioned medium of this cell line is a source of MSA, and the MSA polypeptide family can partially meet the demand of chicken embryo fibroblasts for serum. 这个细胞株的无血清条件培养基是MSA的一个来源，MSA多肽家族能够部分满足鸡胚成纤维细胞对血清的需求。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Positive 阳性 B类 Class B BRL 3A-cell morphology-SHCRC BRL 3A-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 BRL 3A BRL 3A cell BRL 3A 细胞 NICR532 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR532 cell NICR532 细胞 http://www.cellresource.cn/fdetail.aspx?id=532 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf BRL 3A-cell morphology-SHCRC BRL 3A-细胞形态-SHCRC 3131C0001000500010 Rat Hepatocytes; BRL 3A 大鼠肝细胞；BRL 3A adherent growth 贴壁生长 The serum-free conditioned medium of this cell line is a source of MSA. The MSA polypeptide family can partially meet the demand of chicken embryo fibroblasts for serum. 这个细胞株的无血清条件培养基是MSA的一个来源。 MSA多肽家族能够部分满足鸡胚成纤维细胞对血清的需求。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A BRL 3A-cell morphology-SHCRC BRL 3A-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 BRL 3A BRL 3A cell BRL 3A 细胞 NICR1676 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1676 cell NICR1676 细胞 http://www.cellresource.cn/fdetail.aspx?id=1676 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf BRL 3A-cell morphology-SHCRC BRL 3A-细胞形态-SHCRC - - 3111C0001CCC000183 Rat Hepatoma; H-4-II-E 大鼠肝细胞瘤；H-4-II-E epithelioid 上皮样 adherent growth 贴壁生长 Aryl hydrocarbon hydroxylase can be produced by induction for the detection of picocell-grade polychlorinated biphenyl organic compounds in environmental samples or food extracts. 可诱导产生芳烃羟化酶，用于检测环境样品或食物提取物中皮克级的多氯联苯有机化合物。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 44~56 44~56 A类 Class A H-4-II-E-cell morphology-PUMC H-4-II-E-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；2~3天换液1次。 Passage at 1: 2 ~ 1: 4; and change fluid once in 2~3 days. H-4-II-E H-4-II-E cell H-4-II-E 细胞 NICR183 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR183 cell NICR183 细胞 http://www.cellresource.cn/fdetail.aspx?id=183 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf H-4-II-E-cell morphology-PUMC H-4-II-E-细胞形态-PUMC STR Identification STR鉴定 3142C0001000000115 Rat Hepatoma; H-4-II-E 大鼠肝细胞瘤；H-4-II-E epithelial-like 上皮细胞样 adherent growth 贴壁生长 Aryl hydrocarbon hydroxylase can be produced by induction for the detection of picocell-grade polychlorinated biphenyl organic compounds in environmental samples or food extracts. 可诱导产生芳烃羟化酶，用于检测环境样品或食物提取物中皮克级的多氯联苯有机化合物。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C147 C147 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. H-4-II-E H-4-II-E cell H-4-II-E 细胞 NICR883 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR883 cell NICR883 细胞 http://www.cellresource.cn/fdetail.aspx?id=883 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf - - 3111C0001CCC000184 Rat Hepatoma; H4-II-E-C3 大鼠肝细胞瘤；H4-II-E-C3 epithelioid 上皮样 adherent growth 贴壁生长 Tyrosine aminotransferase may be produced under induction of glucocorticoid, insulin or cAMP derivatives; the cells may be infected by retrovirus; the cells may produce albumin, transferrin, prothrombin; and form tumors in Axc rats. 在糖皮质激素、胰岛素或cAMP衍生物的诱导下可以产生酪氨酸氨基转移酶；可被逆转录病毒感染；可产生白蛋白、转铁蛋白、凝血酶原；在AxC大鼠中可以成瘤。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%HS＋5%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%HS＋5%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:12传代；2~3天换液1次。 Passage at 1: 4 ~ 1: 12; and change fluid once in 2~3 days. H4-II-E-C3 H4-II-E-C3 cell H4-II-E-C3 细胞 NICR184 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR184 cell NICR184 细胞 http://www.cellresource.cn/fdetail.aspx?id=184 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%HS＋5%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%HS＋5%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf - - 3111C0001CCC000192 Rat Skeletal Muscle Myoblasts; L6 大鼠骨骼肌成肌细胞；L6 myoblast-like 成肌细胞样 adherent growth 贴壁生长 The cells were isolated by Yaffe from the first two generations of rat thigh muscle primary culture in the presence of methylcholanthrene; the cells fused in culture medium to form polynuclear myotubes and rhabdomyocytes and the fusion rate decreased with the generation increased, so the cells should be frozen for low generations and recloned periodically to select cells with strong fusion power. The cells are ectromelia virus-negative. The cells should be passaged before reaching confluent state to delay the loss of differentiation ability of the cells. 该细胞是Yaffe在甲基胆蒽存在的情况下从大鼠大腿肌原代培养的最初两代细胞中分离得到的；在培养基中融合形成多核的肌管和横纹肌纤维，细胞融合的程度随着代数的增加而下降，因此细胞应低代次冷冻并周期性地重新克隆以选择融合能力强的细胞。鼠痘病毒阴性。该细胞应在达汇合状态前传代，以延缓细胞分化能力的丧失。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 38~40 38~40 A类 Class A L6-Species Identification-PUMC L6-cell morphology-PUMC L6-物种鉴定-PUMC L6-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:20~1:40传代；每周换液2~3次。 Passage at 1: 20 ~ 1:40; and change fluid for 2~3 times a week. L6 L6 cell L6 细胞 NICR192 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR192 cell NICR192 细胞 http://www.cellresource.cn/fdetail.aspx?id=192 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100710.pdf L6-Species Identification-PUMC L6-cell morphology-PUMC L6-物种鉴定-PUMC L6-细胞形态-PUMC 3131C0001000500004 Rat Skeletal Muscle Myoblasts; L6 大鼠骨骼肌成肌细胞；L6 myoblast 成肌细胞 adherent growth 贴壁生长 L6 myoblast strain Yaffe was isolated from first two generations of the primary culture of rat thigh muscle in the presence of methylcholanthrene. The L6 cells are fused in the medium to form polynuclear myotubes and rhabdomyocytes. The fusion degree of the cells decreases with the increase of the generation, so the cells should be frozen in the lower generation stage and recloned periodically to select cells with strong fusion ability. If the cells in the culture vessel are overgrown, the myogenic components of the cells will be rapidly depleted. Detection for the limb bone malformations virus ectromelia virus is negative. L6成肌细胞株是Yaffe从甲基胆蒽存在下大鼠大腿肌的原代培养物最初两代中分离得到。 L6细胞在培养基中融合形成多核的肌管和横纹肌纤维。 细胞融合的程度随着代数的增加而下降，因而细胞应冷冻于低代次阶段并周期性地重新克隆以选择融合能力强的细胞。 如果让培养容器中的细胞长满，这株细胞的成肌组分将迅速耗尽。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A L6-cell morphology-SHCRC L6-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 L6 L6 cell L6 细胞 NICR533 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR533 cell NICR533 细胞 http://www.cellresource.cn/fdetail.aspx?id=533 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf L6-cell morphology-SHCRC L6-细胞形态-SHCRC 3131C0001001200010 Rat Osteosarcoma Cells; UMR-106 大鼠骨肉瘤细胞；UMR-106 epithelioid 上皮样 adherent growth 贴壁生长 The cells is derived from transplantable osteosarcoma which formed in SD rats induced by radionuclide 32P. 该细胞来源于放射性核素32P诱导SD大鼠形成可移植性的骨肉瘤。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B UMR-106-cell morphology-SHCRC UMR-106-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 14~1:8传代，每周2~3次。 Passage at 14 ~ 1: 8, and passage for 2 to 3 times per week. NICR1591 UMR-106 UMR-106 cell UMR-106 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1591 cell NICR1591 细胞 http://www.cellresource.cn/fdetail.aspx?id=1591 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf UMR-106-cell morphology-SHCRC UMR-106-细胞形态-SHCRC 3131C0001001000011 Rat Osteosarcoma Cells; UMR-106 大鼠骨肉瘤细胞；UMR-106 epithelial cell 上皮细胞 adherent growth 贴壁生长 The UMR-106 cell line was established by cloning the transplantable rat osteosarcoma induced by phosphorus radioisotope (32P). The cells respond to PTH, prostaglandins and osteoclast steroids. The response of UMR-106 to PTH was higher than that of UMR-108 (ATCC CRL-1663). Activation of protein kinase C inhibits ATP-induced elevation of the level of intracellular calcium. Both the starting osteosarcoma and the cloned strain were established by T.J. Martin of University of Sheffield. 注射放射性同位素磷(32P)诱导产生的可移植性大鼠骨肉瘤克隆建立了UMR-106细胞株。 细胞对PTH，前列腺素及破骨甾体有响应。 对PTH的响应度，UMR-106比相关细胞株UMR-108(ATCC CRL-1663)要高。 蛋白激酶C的活化抑制ATP诱导的胞内钙水平的升高。 起始骨肉瘤和克隆株都是University of Sheffield的T.J.Martin建立的。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A UMR-106-cell morphology-SHCRC UMR-106-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1728 UMR-106 UMR-106 cell UMR-106 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1728 cell NICR1728 细胞 http://www.cellresource.cn/fdetail.aspx?id=1728 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf UMR-106-cell morphology-SHCRC UMR-106-细胞形态-SHCRC 3131C0001001200008 Rat Myeloma cells; Y3-Ag 1.2.3 大鼠骨髓瘤细胞；Y3-Ag 1.2.3 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This is a derived strain of Myeloma Cell Line S210 and was constructed by C. Milstein. Without the agreement of C. Milstein, the cells can not be sold or provided to the third party. This cell line is resistant to 8-azaguanine and sensitive to HAT, and can be fused with rat B cells to produce rat-rat hybridoma. Detection for limb bone malformations virus ectromelia virus is negative. 这是C. Milstein建立的骨髓瘤细胞株S210的衍生株。 未经C. Milstein同意不得销售或提供给第三方。 这株细胞具8-氮鸟嘌呤抗性，HAT敏感，可与大鼠B细胞融合生产大鼠-大鼠杂交瘤。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Maintain the cell concentration between 1 � 105 ~ 2 � 106cells/ml. 维持细胞浓度在1×105~2×106cells/ml。 NICR1589 Y3-Ag 1.2.3 Y3-Ag 1.2.3 cell Y3-Ag 1.2.3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1589 cell NICR1589 细胞 http://www.cellresource.cn/fdetail.aspx?id=1589 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3131C0001001000010 Rat Myeloma cells; Y3-Ag 1.2.3 大鼠骨髓瘤细胞；Y3-Ag 1.2.3 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This is a derived strain of Myeloma Cell Line S210 and was constructed by C. Milstein. Without the agreement of C. Milstein, the cells can not be sold or provided to the third party. This cell line is resistant to 8-azaguanine and sensitive to HAT, and can be fused with rat B cells to produce rat-rat hybridoma. Detection for limb bone malformations virus ectromelia virus is negative. 这是C. Milstein建立的骨髓瘤细胞株S210的衍生株。 未经C. Milstein同意不得销售或提供给第三方。 这株细胞具8-氮鸟嘌呤抗性，HAT敏感，可与大鼠B细胞融合生产大鼠-大鼠杂交瘤。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Maintain the cell concentration between 1 � 105 ~ 2 � 106cells/ml. 维持细胞浓度在1×105~2×106cells/ml。 NICR2108 Y3-Ag 1.2.3 Y3-Ag 1.2.3 cell Y3-Ag 1.2.3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2108 cell NICR2108 细胞 http://www.cellresource.cn/fdetail.aspx?id=2108 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf - - 3111C0001CCC000131 Rat Brain Glioma Cells; C6 大鼠脑胶质瘤细胞；C6 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 C6 is a rat glioma clone constructed by Benda et al induced by methylnitrosourea through a series of culture in vitro and alternations passage in animal. The cells express S-100, and produce growth hormone; The cells cen produce glycerol 3 phosphate dehydrogenase under the function of glucocorticoid. When cells grow from low density to confluence state, the S-100 production will increase 10-fold. C6是由Benda等用N-亚硝基甲脲诱导的大鼠胶质瘤克隆，并经过一系列的体外培养和动物传代交替后建成的。该细胞表达S-100；产生生长激素；糖皮质激素作用下可以产生磷酸甘油脱氢酶。当细胞从低密度生长至回合状态时，S-100产量增加10倍。 F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS http://www.cellresource.cn/pdf/20080108100736.pdf http://www.cellresource.cn/pdf/20080108100736.pdf P50 P50 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A C6-Species Identification-PUMC C6-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:3传代；2~3天换液1次。 Passage at 1: 2 ~ 1: 3; and change fluid once in 2~3 days. C6 C6 cell C6 细胞 NICR131 PMID: 8971005 PMID: 8971005 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR131 cell NICR131 细胞 http://www.cellresource.cn/fdetail.aspx?id=131 F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS URI: http://www.cellresource.cn/pdf/20080108100736.pdf F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS URI: http://www.cellresource.cn/pdf/20080108100736.pdf C6-Species Identification-PUMC C6-物种鉴定-PUMC 3111C0002000000048 Rat Brain Glioma Cells; C6 大鼠脑胶质瘤细胞；C6 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 C6 is a rat glioma clone constructed by Benda et al induced by methylnitrosourea through a series of culture in vitro and alternations passage in animal. The cells express S-100, and produce growth hormone; The cells cen produce glycerol 3 phosphate dehydrogenase under the function of glucocorticoid. When cells grow from low density to confluence state, the S-100 production will increase 10-fold. C6是由Benda等用N-亚硝基甲脲诱导的大鼠胶质瘤克隆，并经过一系列的体外培养和动物传代交替后建成的。该细胞表达S-100；产生生长激素；糖皮质激素作用下可以产生磷酸甘油脱氢酶。当细胞从低密度生长至回合状态时，S-100产量增加10倍。 F12K horse serum，15% bovine serum，2.5 F12K 马血清，15%，牛血清，2.5 F12K 马血清，15%，牛血清，2.5 http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A C6-cell morphology-NIFDC C6-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1：2~1：3 Passage at 1: 2 ~ 1: 3 C6 C6 cell C6 细胞 NICR420 NICR420 cell NICR420 细胞 http://www.cellresource.cn/fdetail.aspx?id=420 F12K 马血清，15%，牛血清，2.5 URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 马血清，15%，牛血清，2.5 URI: http://www.cellresource.cn/pdf/20080108102725.pdf C6-cell morphology-NIFDC C6-细胞形态-NIFDC 3131C0001001000001 Rat Brain Glioma Cells; C6 大鼠脑胶质瘤细胞；C6 fibroblasts 成纤维细胞 adherent growth 贴壁生长 Glia cell line C6 was cloned from a rat glioma cell line induced by Benda et al., and was established after a series of alternation of in vitro culture and animal passage. When cells grew from low density to full flask, S-100 production increased 10-fold. 胶质细胞株C6是由Benda等用N-nitrosomethylurea诱导的大鼠胶质瘤克隆，并经过一系列的体外培养和动物传代交替后建成的。 当细胞从低密度生长到满瓶时，S-100产量增加10倍。 F12K horse serum，15% qualified fetal bovine serum，2.5% F12K 马血清，15%；优质胎牛血清，2.5% F12K 马血清，15%；优质胎牛血清，2.5% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf P(38+5) P(38+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 C6 C6 cell C6 细胞 NICR758 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR758 cell NICR758 细胞 http://www.cellresource.cn/fdetail.aspx?id=758 F12K 马血清，15%；优质胎牛血清，2.5% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 马血清，15%；优质胎牛血清，2.5% URI: http://www.cellresource.cn/pdf/20080108102725.pdf - - 3111C0001CCC000219 Rat Embryonic Cardiomyocytes; H9c2(2-1) 大鼠胚胎心肌细胞；H9c2(2-1) myoblast-like 成肌细胞样 adherent growth 贴壁生长 The cells were subcloned from clone cell lines of BD1X rat embryonic heart tissue by Kims B and Brandt B, showing many skeletal muscle characteristics. The myoblasts in this cell line can fuse into polynuclear myotubes and respond to the stimulation of acetylcholine. If the serum concentration in the medium drops to 1%, fusion occurs quickly. 该细胞由Kimes B和Brandt B从BD1X大鼠胚胎心脏组织的克隆细胞株亚克隆得到；表现出许多骨骼肌的特性。这个细胞株中的成肌细胞能融合形成多核的肌管，并对乙酰胆碱的刺激发生反应。如果培养基中的血清浓度下降到1%，融合很快发生。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A H9C2（2-1）-Species Identification-PUMC H9C2（2-1）-物种鉴定-PUMC H9c2(2-1)-cell morphology-PUMC H9c2(2-1)-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:6传代；2~3天换液1次。 Passage at 1: 2 ~ 1: 6; and change fluid once in 2~3 days. H9c2(2-1) H9c2(2-1) cell H9c2(2-1) 细胞 NICR219 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR219 cell NICR219 细胞 http://www.cellresource.cn/fdetail.aspx?id=219 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100710.pdf H9C2（2-1）-Species Identification-PUMC H9C2（2-1）-物种鉴定-PUMC H9c2(2-1)-cell morphology-PUMC H9c2(2-1)-细胞形态-PUMC 3131C0001000500005 Rat Embryonic Cardiomyocytes; H9c2(2-1) 大鼠胚胎心肌细胞；H9c2(2-1) myoblast 成肌细胞 adherent growth 贴壁生长 B. Kimes and B. Brandt subcloned H9c2 (2-1) cell lines from clone cell lines derived from BD1X rat embryo heart tissue. It shows many characteristics of skeletal muscle. The myoblasts in this cell line can fuse into polynuclear myotubes and respond to the stimulation of acetylcholine. If the serum concentration in the medium drops to 1%, fusion occurs very quickly. B. Kimes and B. Brandt从源于BD1X大鼠胚胎心脏组织的克隆细胞株亚克隆了H9c2(2-1)细胞株。它表现许多骨骼肌的特性。 这个细胞株中的成肌细胞能融合形成多核的肌管，并对乙酰胆碱的刺激发生反应。 如果培养基中的血清浓度下降到1%，融合发生得很快。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P(13+5) P(13+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A H9C2(2-1)-cell morpholgoy-SHCRC H9C2(2-1)-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 H9c2(2-1) H9c2(2-1) cell H9c2(2-1) 细胞 NICR534 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR534 cell NICR534 细胞 http://www.cellresource.cn/fdetail.aspx?id=534 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf H9C2(2-1)-cell morpholgoy-SHCRC H9C2(2-1)-细胞形态-SHCRC - - 3111C0001CCC000024 Rat Adrenal Pheochromocytoma Cells; PC-12 [PC12] 大鼠肾上腺嗜铬细胞瘤细胞；PC-12 [PC12] polygonal 多角形 loosely adherent, multicellular aggregation 疏松贴壁，有多细胞聚集体 The cell line is derived from the adrenal pheochromocytoma of transplantable male rats; the cells express nerve growth factor (NGF) receptors and can produce reversible neurophenotypes induced by NGF. The cells can produce catecholamine, dopamine and norepinephrine, but do not synthesize epinephrine. 该细胞系来自可移植的雄性大鼠的肾上腺嗜铬细胞瘤；表达神经生长因子(NGF)受体，NGF可诱导该细胞产生可逆性的神经表型。该细胞可产生儿茶酚胺、多巴胺和去甲肾上腺素，但不合成肾上腺素。 RPMI 1640 (w/o Hepes) 5%FBS+10%HS RPMI 1640 (w/o Hepes) 5%FBS+10%HS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 32~40 32~40 A类 Class A PC12-Species Identification-PUMC PC12-cell morphology-PUMC PC12-物种鉴定-PUMC PC12-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；3~4天1次。 Passage at 1: 3; and passage once in 3 to 4 days. NICR24 PC-12 PC-12 cell PC-12 细胞 PC12 PC12 cell PC12 细胞 PMID: 8636125 PMID: 8636125 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR24 cell NICR24 细胞 http://www.cellresource.cn/fdetail.aspx?id=24 RPMI 1640 (w/o Hepes) 5%FBS+10%HS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 5%FBS+10%HS URI: http://www.cellresource.cn/pdf/20080108101040.pdf PC12-Species Identification-PUMC PC12-cell morphology-PUMC PC12-物种鉴定-PUMC PC12-细胞形态-PUMC 3131C0001001000003 Rat Adrenal Pheochromocytoma Cells; PC-12 [PC12] 大鼠肾上腺嗜铬细胞瘤细胞；PC-12 [PC12] polygonal 多角形 loosely adherent, multicellular aggregation 疏松贴壁，有多细胞聚集体 The cell line is derived from the adrenal pheochromocytoma of transplantable male rats. These cells express the nerve growth factor (NGF) receptor. NGF can induce the generation of neurophenotype. These cells do not synthesize epinephrine. 该细胞系来自能移植的雄性大鼠肾上腺嗜铬细胞瘤。 这些细胞表达神经生长因子(NGF)受体。 NGF可诱导产生神经表型。 这些细胞不合成肾上腺素。 RPMI 1640 (w/o Hepes) heat-inactivatedhorse serum，10% qualified fetal bovine serum，5% RPMI 1640 (w/o Hepes) 热灭活马血清，10%；优质胎牛血清，5% RPMI 1640 (w/o Hepes) 热灭活马血清，10%；优质胎牛血清，5% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NICR760 PC-12 PC-12 cell PC-12 细胞 PC12 PC12 cell PC12 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR760 cell NICR760 细胞 http://www.cellresource.cn/fdetail.aspx?id=760 RPMI 1640 (w/o Hepes) 热灭活马血清，10%；优质胎牛血清，5% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 热灭活马血清，10%；优质胎牛血清，5% URI: http://www.cellresource.cn/pdf/20080108101040.pdf STR Identification STR鉴定 3142C0001000000006 Rat Adrenal Pheochromocytoma Cells; PC-12 [PC12] 大鼠肾上腺嗜铬细胞瘤细胞；PC-12 [PC12] polymorphic cell-like 多形态细胞样 loosely adherent, multicellular aggregation 疏松贴壁，有多细胞聚集体 The cell line is derived from the adrenal pheochromocytoma of transplantable male rats; the cells express nerve growth factor (NGF) receptors and can produce reversible neurophenotypes induced by NGF. The cells can produce catecholamine, dopamine and norepinephrine, but do not synthesize epinephrine. 该细胞系来自可移植的雄性大鼠的肾上腺嗜铬细胞瘤；表达神经生长因子(NGF)受体，NGF可诱导该细胞产生可逆性的神经表型。该细胞可产生儿茶酚胺、多巴胺和去甲肾上腺素，但不合成肾上腺素。  RPMI 1640 (w/o Hepes)� 10%FBS RPMI 1640 (w/o Hepes)� 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C11 C11 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A PC-12-Myoplasma Detection-CCTCC PC-12-支原体检测-CCTCC PC12-cell morphology-CCTCC PC12-细胞形态-CCTCC Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR774 PC-12 PC-12 cell PC-12 细胞 PC12 PC12 cell PC12 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR774 cell NICR774 细胞 http://www.cellresource.cn/fdetail.aspx?id=774 RPMI 1640 (w/o Hepes)� 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes)� 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf PC-12-Myoplasma Detection-CCTCC PC-12-支原体检测-CCTCC PC12-cell morphology-CCTCC PC12-细胞形态-CCTCC - - 3153C0001000000030 Rat Adrenal Pheochromocytoma Cells; PC-12 [PC12] 大鼠肾上腺嗜铬细胞瘤细胞；PC-12 [PC12] polygonal 多角形 loosely adherent, multicellular aggregation 疏松贴壁，有多细胞聚集体 The cell line is derived from the adrenal pheochromocytoma of transplantable male rats; the cells express nerve growth factor (NGF) receptors and can produce reversible neurophenotypes induced by NGF. The cells can produce catecholamine, dopamine and norepinephrine, but do not synthesize epinephrine. 该细胞系来自可移植的雄性大鼠的肾上腺嗜铬细胞瘤；表达神经生长因子(NGF)受体，NGF可诱导该细胞产生可逆性的神经表型。该细胞可产生儿茶酚胺、多巴胺和去甲肾上腺素，但不合成肾上腺素。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN4 PN4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） A类 Class A PC-12-cell morphology-KCB PC-12-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1：3传代；3-4天一次 Passage at 1: 3; and passage once in 3-4 days NICR1304 PC-12 PC-12 cell PC-12 细胞 PC12 PC12 cell PC12 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1304 cell NICR1304 细胞 http://www.cellresource.cn/fdetail.aspx?id=1304 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf PC-12-cell morphology-KCB PC-12-细胞形态-KCB - - 3111C0001CCC000415 Rat Mesangial Cells; RMC 大鼠肾系膜细胞；RMC adherent growth 贴壁生长 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS + 1% NEAA+ 500ug/ml G418 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS + 1% NEAA+ 500ug/ml G418 http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P78 P78 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com NICR2034 RMC RMC cell RMC 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2034 cell NICR2034 细胞 http://www.cellresource.cn/fdetail.aspx?id=2034 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS + 1% NEAA+ 500ug/ml G418 URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS + 1% NEAA+ 500ug/ml G418 URI: http://www.cellresource.cn/pdf/20080108100710.pdf - - 3111C0001CCC000203 Rat Basophilic Leukemia Cells; RBL-1 大鼠嗜碱性粒细胞性白血病细胞；RBL-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells showes a variety of basophil differentiation characteristics: Histamine release, and the expression of IgE Fc receptor. 该细胞表现出多种嗜碱性粒细胞的分化特征：释放组胺，表达IgE的Fc受体。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration in the 0.1 ~ 1 � 105 cells/ml; and change fluid once in 2 to 3 days. 维持细胞浓度在0.1～1×105 cells/ml；2～3天换液1次。 NICR203 RBL-1 RBL-1 cell RBL-1 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR203 cell NICR203 细胞 http://www.cellresource.cn/fdetail.aspx?id=203 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3142C0001000000341 Rat Basophilic Leukemia Cells; RBL-1 大鼠嗜碱性粒细胞性白血病细胞；RBL-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 RBL-1 cells were isolated and established from peripheral blood of a leukemia patient by H Metzger and C Isersky. The cells exhibit many cellular characteristics of basophil, such as the surface of cells carrying IgE receptors. However, in IgE-mediated responses, the cells do not release histamine. RBL-1细胞由H Metzger和C Isersky由一名白血病患者的外周血中分离并建立，细胞表现出嗜碱性粒多种细胞特性，例如细胞表面带有IgE受体.但在IgE介导的反应中，该细胞并不释放组织胺。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C6 C6 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR1992 RBL-1 RBL-1 cell RBL-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1992 cell NICR1992 细胞 http://www.cellresource.cn/fdetail.aspx?id=1992 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001001000007 Rat Basophilic Leukemia Cells; RBL-2H3 大鼠嗜碱性粒细胞性白血病细胞；RBL-2H3 fibroblasts 成纤维细胞 adherent growth 贴壁生长 RBL-2H3 is an alkaline leukemia cell line isolated and cloned from the tumor-preserving basophilic cells of Wistar rats by the Immunology Laboratory of the National Dental Research Institute in 1978. These cells have high affinity for IgE receptors. Histamine and other neurotransmitter secreted by these receptor can be activated by aggregation of these receptors or by synergism with calcium ionophore. This cell line is widely used to study the FcERI and secretion biochemical pathways of mast cells. RBL-2H3 cells are the research model for FcERI structure. They are widely used to study different aspects of cell secretion, including changes in intracellular calcium concentration, lipoxygenase activation, protein kinases and the action of small G proteins. Although almost all batches of FBS support cell growth, FcERI aggregates and de-particles are better in some batches . Another rat basophilic cell line (RBL, ATCC CRL-1378) is not de-granulated. Mycoplasma has been detected in this library. RBL-2H3是1978年国立牙科研究所的免疫学实验室从Wistar大鼠保持肿瘤状态的嗜碱性细胞中分离和克隆出来的嗜碱性白血病细胞株。 这些细胞具有高亲和力的IgE受体。 通过集聚这些受体或与钙离子载体协同作用可以激活它们分泌组胺及其他递质。 这株细胞广泛地用于研究肥大细胞FcERI和分泌的生化途径。 RBL-2H3细胞是研究FcERI结构的模型。 它们广泛地用于研究细胞分泌的不同方面，包括细胞内钙浓度改变、磷酸脂酶激活、蛋白激酶和小G蛋白的作用。 虽然几乎所有批号的FBS都支持细胞的生长，但在某些批号 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified heat-inactivatedfetal bovine serum，15% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，15% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，15% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A RBL-2H3-cell morphology RBL-2H3-细胞形态 Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR764 RBL-2H3 RBL-2H3 cell RBL-2H3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR764 cell NICR764 细胞 http://www.cellresource.cn/fdetail.aspx?id=764 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，15% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，15% URI: http://www.cellresource.cn/pdf/20080108100951.pdf RBL-2H3-cell morphology RBL-2H3-细胞形态 - - 3111C0001CCC000110 Rat Small Intestinal Crypt Epithelial Cells; IEC-6 大鼠小肠隐窝上皮细胞；IEC-6 epithelioid 上皮样 adherent growth 贴壁生长 Cortisol can inhibit the growth of the cells. The cells express intestinal epithelial-specific antigen and maintained a constant growth rate and cell morphology before the 20th passage, after which the growth rate rapidly decreased and the cell morphology also changed. 皮质醇可抑制该细胞的生长。该细胞表达肠上皮特有抗原，在20代以前保持恒定的生长速度和细胞形态，此后生长速度迅速下降，细胞形态也发生改变。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 5%FBS；0.01mg/mlInsulin DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 5%FBS；0.01mg/mlInsulin http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P24 P24 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 40~51 40~51 A类 Class A IEC-6-Species Identification-PUMC IEC-6-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；每周换液2次。 Passage at 1: 3 ~ 1: 6; and change fluid for 2 times a week. IEC-6 IEC-6 cell IEC-6 细胞 NICR110 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR110 cell NICR110 细胞 http://www.cellresource.cn/fdetail.aspx?id=110 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 5%FBS；0.01mg/mlInsulin URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 5%FBS；0.01mg/mlInsulin URI: http://www.cellresource.cn/pdf/20080108100710.pdf IEC-6-Species Identification-PUMC IEC-6-物种鉴定-PUMC 3131C0001000500007 Rat Thoracic Aorta Smooth Muscle Cells; A7r5 大鼠胸大动脉平滑肌细胞；A7r5 adherent growth 贴壁生长 Rat thoracic large artery smooth muscle cells; adherent growth; 大鼠胸大动脉平滑肌细胞；贴壁生长； PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B A7r5-cell morphology-SHCRC A7r5-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn A7r5 A7r5 cell A7r5 细胞 NICR536 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR536 cell NICR536 细胞 http://www.cellresource.cn/fdetail.aspx?id=536 A7r5-cell morphology-SHCRC A7r5-细胞形态-SHCRC 3131C0001000500006 Rat Schwann Cells; RSC96 大鼠雪旺细胞；RSC96 neurons 神经元 adherent growth 贴壁生长 RSC96 cell line was produced by spontaneous transformation of primary cultured rat Schwann cells after a long time culture[PubMed: 9766530]. Cultures submitted to ATCC in December 2002 were contaminated with mycoplasma. The offspring were treated with BM cyclin for 21 days to eliminate mycoplasma. After treatment for six weeks, mycoplasma detection was performed with Hoechst staining, PCR and standard culture tests for mycoplasma detection. The results were all negative for the former tests. RSC96细胞株是原代培养的大鼠雪旺细胞经长时间培养后自发转化而成的[PubMed: 9766530]。 2002年十二月提交到ATCC的培养物污染了支原体。 其后代通过BM 细胞周期蛋白处理21天消除支原体。 处理后六周，用Hoechst染色、PCR和标准培养测试进行支原体检测。 结果都呈阴性。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR535 RSC96 RSC96 cell RSC96 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR535 cell NICR535 细胞 http://www.cellresource.cn/fdetail.aspx?id=535 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf - - 3111C0001CCC000386 Rat Islet β Tumor Cells; RIN-m5F 大鼠胰岛β细胞瘤细胞；RIN-m5F epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell is a clone of rat pancreatic islet cell RIN-m, which secretes insulin and L-dopa decarboxylase and does not produce growth hormone-inhibiting hormone. 该细胞是大鼠胰岛细胞RIN-m的克隆，分泌胰岛素及L型多巴脱羧酶，不产生生长激素抑制激素。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A RINm5F-Species Identification-CRC RINm5F-物种鉴定-CRC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3～1:6传代，每周换液2～3次 Passage at 1: 3 ~ 1: 6, and change fluid for 2 to 3 times a week NICR1575 RIN-m5F RIN-m5F cell RIN-m5F 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1575 cell NICR1575 细胞 http://www.cellresource.cn/fdetail.aspx?id=1575 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RINm5F-Species Identification-CRC RINm5F-物种鉴定-CRC 3131C0001001000006 Rat Pancreatic Acinar Cells; AR42J 大鼠胰腺外分泌腺肿瘤细胞；AR42J epithelial cell 上皮细胞 adherent growth 贴壁生长 The exocrine activity can be induced under the stimulation of corticosteroid and is accompanied by extensive endoplasmic reticulum remodeling. 在肾上腺皮质激素刺激下可诱导出外分泌活性，并伴有广泛的内质网重构。 F12K qualified fetal bovine serum，20% F12K 优质胎牛血清，20% F12K 优质胎牛血清，20% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf P(19+5) P(19+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 AR42J AR42J cell AR42J 细胞 NICR763 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR763 cell NICR763 细胞 http://www.cellresource.cn/fdetail.aspx?id=763 F12K 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108102725.pdf STR Identification STR鉴定 3142C0001000000134 Rat Pancreatic Acinar Cells; AR42J 大鼠胰腺外分泌腺肿瘤细胞；AR42J epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were derived from pancreatic extrapancreatic gonad tumors in rats and were established by N.W. Jessop in 1980. The cells express a large amount of amylase and other extracellular secretase and form tumors in thymic-free mice. 该细胞源自大鼠胰外分泌性腺肿瘤，由N.W.Jessop于1980年建系。该细胞表达大量的淀粉酶及其它胞外分泌酶，在无胸腺小鼠中成瘤。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C24 C24 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. AR42J AR42J cell AR42J 细胞 NICR902 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR902 cell NICR902 细胞 http://www.cellresource.cn/fdetail.aspx?id=902 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf - - 3111C0001CCC000003 Dihydrofolate Reductase-deficient Chinese Hamster Ovary Cells; CHO/dhFr- 二氢叶酸还原酶缺陷型中国仓鼠卵巢细胞；CHO/dhFr- epithelioid 上皮样 adherent growth 贴壁生长 The cells are dihydrofolate reductase-deficient; the cells will die in the absence of HT (hypoxanthine-thymine) in the medium; the addition of methotrexate in the medium can prevent the growth of recurrent cells with low resistance to drugs; the cells can be used as transfected hosts. 该细胞为二氢叶酸还原酶缺陷型；培养基中没有HT（次黄嘌呤-胸腺嘧啶）时，该细胞会死亡；培养基中加入氨甲喋呤可以防止对该药物具有低抵抗性的回变细胞的生长；可作转染宿主。 F12: Ham＇s F12 Nutrient Mixture 10%FBS F12: Ham＇s F12 Nutrient Mixture 10%FBS http://www.cellresource.cn/pdf/20080108100810.pdf http://www.cellresource.cn/pdf/20080108100810.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 18~25 18~25 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代；每周2~3次。 Passage at 1: 4 ~ 1: 8; and passage for 2 to 3 times per week. CHO/dhFr- CHO/dhFr- cell CHO/dhFr- 细胞 NICR3 PMID: 9226167 PMID: 9226167 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR3 cell NICR3 细胞 http://www.cellresource.cn/fdetail.aspx?id=3 F12: Ham＇s F12 Nutrient Mixture 10%FBS URI: http://www.cellresource.cn/pdf/20080108100810.pdf F12: Ham＇s F12 Nutrient Mixture 10%FBS URI: http://www.cellresource.cn/pdf/20080108100810.pdf 3131C0001000100004 Dihydrofolate Reductase-deficient Chinese Hamster Ovary Cells; CHO/dhFr- 二氢叶酸还原酶缺陷型中国仓鼠卵巢细胞；CHO/dhFr- epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell is a dihydrofolate reductase deficient cell. The cells will die in the absence of HT (hypoxanthine-thymine). The addition of methotrexate to the cell culture can prevent the growth of recurrent cells with low resistance to drugs. 该细胞为二氢叶酸还原酶缺陷细胞。 在没有HT（次黄嘌呤-胸腺嘧啶）时细胞会死亡。 细胞培液中加氨甲喋呤可以防止对药物低抗性的回变细胞的生长。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，10%；0.1 mM hypoxanthine, 0.016 mM thymidine, 100 nM methotrexate IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10%；0.1mM次黄嘌呤，0.016mM胸腺嘧啶，100nM氨甲喋呤 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10%；0.1mM次黄嘌呤，0.016mM胸腺嘧啶，100nM氨甲喋呤 http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 CHO/dhFr- CHO/dhFr- cell CHO/dhFr- 细胞 NICR456 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR456 cell NICR456 细胞 http://www.cellresource.cn/fdetail.aspx?id=456 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10%；0.1mM次黄嘌呤，0.016mM胸腺嘧啶，100nM氨甲喋呤 URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10%；0.1mM次黄嘌呤，0.016mM胸腺嘧啶，100nM氨甲喋呤 URI: http://www.cellresource.cn/pdf/20080108100822.pdf STR Identification STR鉴定 3142C0001000000092 Dihydrofolate Reductase-deficient Chinese Hamster Ovary Cells; CHO/dhFr- 二氢叶酸还原酶缺陷型中国仓鼠卵巢细胞；CHO/dhFr- epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are dihydrofolate reductase-deficient; the cells will die in the absence of HT (hypoxanthine-thymine) in the medium; the addition of methotrexate in the medium can prevent the growth of recurrent cells with low resistance to drugs; the cells can be used as transfected hosts. 该细胞为二氢叶酸还原酶缺陷型；培养基中没有HT（次黄嘌呤-胸腺嘧啶）时，该细胞会死亡；培养基中加入氨甲喋呤可以防止对该药物具有低抵抗性的回变细胞的生长；可作转染宿主。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf C75 C75 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. CHO/dhFr- CHO/dhFr- cell CHO/dhFr- 细胞 NICR860 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR860 cell NICR860 细胞 http://www.cellresource.cn/fdetail.aspx?id=860 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf - - 3153C0001000000217 African Green Monkey Kidney Cells; marc145 非洲绿猴胚胎肾细胞；marc145 epithelioid cells 上皮样细胞 adherent growth 贴壁生长 The cell line is derived from embryonic kidney tissue of an African green monkey. 该细胞系来源于一非洲绿猴的胚胎肾组织。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） None 无 88-104 88-104 A类 Class A marc145-cell morphology-KCB marc145-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1:2传代；3-4天一次 Passage at 1: 2; and passage once in 3-4 days NICR2168 marc145 marc145 cell marc145 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2168 cell NICR2168 细胞 http://www.cellresource.cn/fdetail.aspx?id=2168 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf marc145-cell morphology-KCB marc145-细胞形态-KCB - - 3111C0001CCC000098 African Green Monkey Kidney Cells; BS-C-1 非洲绿猴肾细胞；BS-C-1 epithelioid 上皮样 adherent growth 贴壁生长 The cells express keratin and are suitable for SV40 vector transfection. 表达角蛋白,适于SV40载体转染。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P53 P53 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 110� 110� A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；每周换液2~3次。 Passage at 1: 3 ~ 1: 6; and change fluid for 2~3 times a week. BS-C-1 BS-C-1 cell BS-C-1 细胞 NICR98 PMID: 14215895 PMID: 14215895 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR98 cell NICR98 细胞 http://www.cellresource.cn/fdetail.aspx?id=98 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf STR Identification STR鉴定 3142C0001000000033 African Green Monkey Kidney Cells; BS-C-1 非洲绿猴肾细胞；BS-C-1 epithelial-like 上皮细胞样 adherent growth 贴壁生长 BSC-1 cells were established in 1961 (Hopps, H E, et al). It is sensitive to type Poliovirus type 1, SV40 virus (SV40 virus) and Vesicular stomatitis virus, and is a useful material for studies on virus diagnosis. In addition, BSC-1 cells are also ideal hosts for viral transfection, in particular for SV40 viral vector transfection. BSC-1细胞建系于1961年（Hopps，H E，et al）。它对1型脊髓灰质炎病毒（Poliovirus type 1），SV40病毒（SV40 virus），水泡性口炎病毒（Vesicular stomatitis virus）敏感，是病毒诊断研究的有用材料。另外，BSC-1细胞还是病毒转染，尤其是SV40 病毒载体转染的理想宿主。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C60 C60 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. BS-C-1 BS-C-1 cell BS-C-1 细胞 NICR801 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR801 cell NICR801 细胞 http://www.cellresource.cn/fdetail.aspx?id=801 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3111C0002000000094 African Green Monkey Kidney Cells; BS-C-1 非洲绿猴肾细胞；BS-C-1 epithelioid 上皮样 adherent growth 贴壁生长 The cells are sensitive to SV40, polio 1 and so on. They are used for virus detection and infection study. 该细胞对SV40，脊灰1等敏感，用于病毒检测及感染研究。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P49 P49 90%FBS+10%DMSO 90%FBS+10%DMSO Culture Method（-） 培养法（-） A类 Class A BS-C-1-cell morphology-NIFDC BS-C-1-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:3-1:6，每周换液2-3次 Passage at 1: 3-1: 6, and change fluid for 2-3 times a week BS-C-1 BS-C-1 cell BS-C-1 细胞 NICR2119 NICR2119 cell NICR2119 细胞 http://www.cellresource.cn/fdetail.aspx?id=2119 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf BS-C-1-cell morphology-NIFDC BS-C-1-细胞形态-NIFDC - - 3111C0001CCC000064 African Green Monkey Kidney Cells; CV-1 非洲绿猴肾细胞；CV-1 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 CV-1 cell line was established in 1964 by Jensen FC et al. It originated from adult male African green monkey kidney and was used for transfection of Rous sarcoma virus. It can be used as transfection host for SV40 vector. CV-1细胞株是1964年由Jensen FC等建系的，源自成年雄性非洲绿猴肾，被用于Rous肉瘤病毒的转染研究。可作为SV40载体的转染宿主。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 66~67，100~130 66~67，100~130 A类 Class A CV-1-Species Identification-PUMC CV-1-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:3传代；每周换液2~3次。 Passage at 1: 2 ~ 1: 3; and change fluid for 2~3 times a week. CV-1 CV-1 cell CV-1 细胞 NICR64 PMID: 8985401 PMID: 8985401 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR64 cell NICR64 细胞 http://www.cellresource.cn/fdetail.aspx?id=64 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf CV-1-Species Identification-PUMC CV-1-物种鉴定-PUMC 3111C0002000000020 African Green Monkey Kidney Cells; CV-1 非洲绿猴肾细胞；CV-1 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 CV-1 cell line was established in 1964 by Jensen FC et al. It originated from adult male African green monkey kidney and was used for transfection of Rous sarcoma virus. It can be used as transfection host for SV40 vector. CV-1细胞株是1964年由Jensen FC等建系的，源自成年雄性非洲绿猴肾，被用于Rous肉瘤病毒的转染研究。可作为SV40载体的转染宿主。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) new-born calf serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A CV-1-cell morphology-NIFDC CV-1-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:2-1:3 Passage at 1: 2-1: 3 CV-1 CV-1 cell CV-1 细胞 NICR392 NICR392 cell NICR392 细胞 http://www.cellresource.cn/fdetail.aspx?id=392 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf CV-1-cell morphology-NIFDC CV-1-细胞形态-NIFDC 3131C0001000400011 African Green Monkey Kidney Cells; CV-1 非洲绿猴肾细胞；CV-1 fibroblasts 成纤维细胞 adherent growth 贴壁生长 CV-1 cell line was derived from the kidney of adult male African green monkey and was used for transfection of Rous sarcoma virus. CV-1细胞株源自成年雄性非洲绿猴的肾，用于Rous肉瘤病毒的转染研究。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 CV-1 CV-1 cell CV-1 细胞 NICR515 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR515 cell NICR515 细胞 http://www.cellresource.cn/fdetail.aspx?id=515 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf - - 3111C0001CCC000060 African Green Monkey Kidney Cells; VERO 非洲绿猴肾细胞；VERO epithelioid cells 上皮样细胞 adherent growth 贴壁生长 Vero cell lines were isolated from kidney tissues of normal adult African green monkeys by Yasumura Y and Kawakita Y from Chiba University in Japan. The cells are often used as transfection hosts for the detection of mycoplasma. Vero细胞株是日本千叶大学的Yasumura Y和Kawakita Y从正常成年非洲绿猴的肾脏组织中分离建立的。该细胞常作为转染宿主，用于支原体的检测。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P45 P45 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 49~58 49~58 A类 Class A VERO-Cell Morphology-PUMC VERO-Species Identification-PUMC VERO-cell morphology-PUMC VERO-物种鉴定-PUMC VERO-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:8传代；每周2~3次。 Passage at 1: 8; and passage for 2 to 3 times per week. NICR60 VERO VERO cell VERO 细胞 PMID: 8627697 PMID: 8627697 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR60 cell NICR60 细胞 http://www.cellresource.cn/fdetail.aspx?id=60 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf VERO-Cell Morphology-PUMC VERO-Species Identification-PUMC VERO-cell morphology-PUMC VERO-物种鉴定-PUMC VERO-细胞形态-PUMC VERO-细胞形态-PUMC 3111C0002000000001 African Green Monkey Kidney Cells; VERO 非洲绿猴肾细胞；VERO epithelioid cells 上皮样细胞 adherent growth 贴壁生长 Vero cell lines were isolated from kidney tissues of normal adult African green monkeys by Yasumura Y and Kawakita Y from Chiba University in Japan. The cells are often used as transfection hosts for the detection of mycoplasma. Vero细胞株是日本千叶大学的Yasumura Y和Kawakita Y从正常成年非洲绿猴的肾脏组织中分离建立的。该细胞常作为转染宿主，用于支原体的检测。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) new-born calf serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P125-P165 P125-P165 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Vero-cell morphology-NIFDC Vero-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:3-1:6 Passage at 1: 3-1: 6 NICR373 VERO VERO cell VERO 细胞 NICR373 cell NICR373 细胞 http://www.cellresource.cn/fdetail.aspx?id=373 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf Vero-cell morphology-NIFDC Vero-细胞形态-NIFDC 3131C0001000400010 African Green Monkey Kidney Cells; VERO 非洲绿猴肾细胞；VERO epithelial cell 上皮细胞 adherent growth 贴壁生长 Vero cell line was constructed from the kidneys of normal adult African green monkeys by Y. Yasumura and Y. Kawakita of Chiba University, Japan. B. Simizu took it from Chiba University to the National Institute of Allergy and Infectious Diseases Tropical Virus Laboratorythe, National Institutes of Health (NIH), and passaged it to the 93rd generation on June 15, 1964. Vero细胞株是日本千叶大学的Y. Yasumura和Y. Kawakita从正常成年非洲绿猴的肾脏建株的。1964年6月15日B. Simizu将其从千叶大学带到国立健康研究所(NIH)国立过敏及传染病研究所热带病毒实验室时，已传至第93代。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) fetal bovine serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(19+5) P(19+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A LDH检测报告Vero-Isoenzyme-SHCRC LDH检测报告Vero-同工酶-SHCRC MDH检测报告Vero-Isoenzyme-SHCRC MDH检测报告Vero-同工酶-SHCRC VERO-cell morphology-SHCRC VERO-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR514 VERO VERO cell VERO 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR514 cell NICR514 细胞 http://www.cellresource.cn/fdetail.aspx?id=514 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf LDH检测报告Vero-Isoenzyme-SHCRC LDH检测报告Vero-同工酶-SHCRC MDH检测报告Vero-Isoenzyme-SHCRC MDH检测报告Vero-同工酶-SHCRC VERO-cell morphology-SHCRC VERO-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000029 African Green Monkey Kidney Cells; VERO 非洲绿猴肾细胞；VERO epithelial-like 上皮细胞样 adherent growth 贴壁生长 Vero cell lines were isolated from kidney tissues of normal adult African green monkeys by Yasumura Y and Kawakita Y from Chiba University in Japan. The cells are often used as transfection hosts for the detection of mycoplasma and for the fundamental research of many viruses. Vero细胞株是日本千叶大学的Yasumura Y和Kawakita Y从正常成年非洲绿猴的肾脏组织中分离建立的。该细胞常作为转染宿主，用于支原体的检测，也可用于多种病毒的基础研究。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C127 C127 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR797 VERO VERO cell VERO 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR797 cell NICR797 细胞 http://www.cellresource.cn/fdetail.aspx?id=797 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf - - 3153C0001000000035 African Green Monkey Kidney Cells; VERO 非洲绿猴肾细胞；VERO epithelium-like, polygonal shape 上皮样，多角形 adherent growth 贴壁生长 Vero cell lines were isolated from kidney tissues of normal adult African green monkeys by Yasumura Y and Kawakita Y from Chiba University in Japan. The cells are often used as transfection hosts for the detection of mycoplasma. Vero细胞株是日本千叶大学的Yasumura Y和Kawakita Y从正常成年非洲绿猴的肾脏组织中分离建立的。该细胞常作为转染宿主，用于支原体的检测。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN3 PN3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） 54-61 54-61 A类 Class A VERO-cell morphology-KCB VERO-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1：2传代；3-4天一次 Passage at 1: 2; and passage once in 3-4 days NICR1309 VERO VERO cell VERO 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1309 cell NICR1309 细胞 http://www.cellresource.cn/fdetail.aspx?id=1309 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%NBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf VERO-cell morphology-KCB VERO-细胞形态-KCB 3131C0001000400017 African Green Monkey Kidney Cells; VERO C1008 (E6) 非洲绿猴肾细胞；VERO C1008 (E6) epithelial cell 上皮细胞 adherent growth 贴壁生长 VERO C1008 is a cloned cell line of VERO 76, while VERO 76 is a derivative of the initial VERO cell line. VERO C1008是VERO 76的克隆细胞株，而VERO 76是初始VERO细胞株的一个衍生株。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A VERO C1008(E6)-cell morphology-SHCRC VERO C1008(E6)-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1672 VERO C1008 (E6) VERO C1008 (E6) cell VERO C1008 (E6) 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1672 cell NICR1672 细胞 http://www.cellresource.cn/fdetail.aspx?id=1672 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf VERO C1008(E6)-cell morphology-SHCRC VERO C1008(E6)-细胞形态-SHCRC - - 3111C0001CCC000388 African Green Monkey Kidney Cells; Vero E6 非洲绿猴肾细胞；Vero E6 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell is a clone of VERO 76 cells and obtianded by P.J. Price by finite dilution method in 1979. 该细胞是VERO 76 细胞的一个克隆，1979年由P.J. Price通过有限稀释法获得。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P25 P25 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4传代，每周换液2～3次 Passage at 1: 4, and change fluid for 2 to 3 times a week NICR1577 Vero E6 Vero E6 cell Vero E6 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1577 cell NICR1577 细胞 http://www.cellresource.cn/fdetail.aspx?id=1577 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3111C0002000000007 African Green Monkey Kidney Cells; VERO-76 非洲绿猴肾细胞；VERO-76 epithelioid cells 上皮样细胞 adherent growth 贴壁生长 It is a subclone of VERO cells. 为VERO细胞的一个亚克隆 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) new-born calf serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Vero76-cell morphology-NIFDC Vero76-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1：8,4-5天一次 Passage at 1: 8,and passage once in 4-5 days NICR379 VERO-76 VERO-76 cell VERO-76 细胞 NICR379 cell NICR379 细胞 http://www.cellresource.cn/fdetail.aspx?id=379 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf Vero76-cell morphology-NIFDC Vero76-细胞形态-NIFDC 3131C0001000400028 African Green Monkey Kidney Cells (SV40-Transformed Cells); COS-1 [COS1] 非洲绿猴肾细胞（SV40转化）；COS-1 [COS1] fibroblasts 成纤维细胞 adherent growth 贴壁生长 This cell line is derived from a CV-1 cell line and is transfected with SV40 with an initial inactivation mutation encoding the wild-type T antigen. These cells contain a constitutive copy from the entire early region of the SV40 genome. 此细胞株源自CV-1细胞株，经带有编码野生型T抗原的起始失活突变的SV40转染得到。 这些细胞含有一个来自SV40基因组全部早期区域的组成型拷贝。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 COS-1 COS-1 cell COS-1 细胞 COS1 COS1 cell COS1 细胞 NICR1673 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1673 cell NICR1673 细胞 http://www.cellresource.cn/fdetail.aspx?id=1673 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3131C0001000400020 Opossum Kidney Proximal Tubular Cells; OK 负鼠肾近曲小管上皮细胞；OK epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell line initially provided an X chromosome for the study of X chromosome inactivation, and was subsequently found to be an excellent cell culture model of renal proximal tubule epithelial cells. This cell expresses a variety of receptors in culture and is widely used as a model for studying these receptors. 此细胞株最初是为研究X染色体失活时提供X染色体，随后发现它是极好的肾近曲小管上皮细胞的细胞培养模型。此细胞在培养时表达多种受体，并被广泛地用作研究这些受体的模型。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(36+5) P(36+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR523 OK OK cell OK 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR523 cell NICR523 细胞 http://www.cellresource.cn/fdetail.aspx?id=523 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000900003 Canine Histiocytic Sarcoma Cells; DH82 狗肾恶性组织细胞增生症细胞；DH82 megakaryocyte 巨噬细胞 adherent growth 贴壁生长 DH82 cells have a macrophage-like morphology and can devour latex particles. The cells were positive in Fc gamma receptor test and negative in Fc mu and C3b receptor test. The cells did not produce IL-1. DH82细胞具有类似巨噬细胞形态，并能吞噬乳胶颗粒。 细胞具有Fc gamma受体阳性，Fc mu和C3b受体阴性。 细胞不生成IL-1。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 DH82 DH82 cell DH82 细胞 NICR1725 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1725 cell NICR1725 细胞 http://www.cellresource.cn/fdetail.aspx?id=1725 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000400029 Dog Kidney Cells; Super Tube 狗肾细胞；Super Tube epithelial cell 上皮细胞 adherent growth 贴壁生长 The cell line MDCK (ATCC CCL-34) was established from a normal adult female canker dog, from which the dog epithelioid cell line Super Tube was cloned. When maintaining a high cell concentration, Super tube formed a large number of tubules (it was reported that 7 x 105 cells placed on each well of a 24-well-plate were able to form tubules within 3 days). To form tubules, the cells culture medium needed to be exchanged twice a day to provide adequate nutrients. The detection level of c-AMP in Super Tube was much lower than that in the original cell line, and adenylyl cyclase was also lower under forskolin stimulation, and its transdermal resistance was also higher than that of Super Dome (ATCC CRL- 2286) and MOCK. 从表观正常的成年雌性可卡犬中建立了细胞株MDCK (ATCC CCL-34)，从中克隆建立了狗上皮样细胞株Super Tube。 当维持高细胞浓度时，Super tube形成大量的小管(据报道，24孔板的每孔铺7x105细胞，３天内就能形成小管)。 要形成小管，细胞需要每天两次换液以提供足够养分。 与原始细胞株相比，Super Tube的c-AMP的检测水平低得多，在forskolin刺激下读出的腺苷环化酶水平也低；它的跨上皮抗性也比Super Dome (ATCC CRL- 2286) 和MDCK都低。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) qualified fetal bovine serum，10% DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10% DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1674 Super Tube Super Tube cell Super Tube 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1674 cell NICR1674 细胞 http://www.cellresource.cn/fdetail.aspx?id=1674 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100542.pdf - - 3111C0001CCC000225 Dog Kidney Cells Protuberance-type; MDCK superdome 狗肾细胞隆突型；MDCK superdome epithelioid 上皮样 adherent growth 贴壁生长 MDCK is derived from dog kidney cells and can form carina. 来源于狗肾细胞MDCK，可以形成隆突。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:6~1:10传代；2~3天1次。 Passage at 1: 6 ~ 1:10; and passage once in 2 to 3 days. MDCK superdome MDCK superdome cell MDCK superdome 细胞 NICR225 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR225 cell NICR225 细胞 http://www.cellresource.cn/fdetail.aspx?id=225 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf - - 3111C0001CCC000516 Drosophila Embryonic Cells; S2 果蝇胚胎细胞；S2 Schneider＇s + 10%FBS(inactivated)，28℃ Schneider＇s + 10%FBS(灭活)，28℃ Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） B类 Class B Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com NICR2437 S2 S2 cell S2 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2437 cell NICR2437 细胞 http://www.cellresource.cn/fdetail.aspx?id=2437 Amelogenin：X，Y；CSF1PO：10，12；D13S317：13；D16S539：8，11；D18S51：17；D19S433：14，14.2；D21S11：28，31；D2S1338：22；D3S1358：15，16；D5S818：10，13；D7S820：10；D8S1179：14，15；FGA：19，27；TH01：6，7；TPOX：8，13；vWA：16，19； Amelogenin：X，Y；CSF1PO：10，12；D13S317：13；D16S539：8，11；D18S51：17；D19S433：14，14.2；D21S11：28，31；D2S1338：22；D3S1358：15，16；D5S818：10，13；D7S820：10；D8S1179：14，15；FGA：19，27；TH01：6，7；TPOX：8，13；vWA：16，19； 3111C0001CCC000046 Burkitt's Lymphoma Cells; RAJI 黑人Burkitt淋巴瘤细胞；RAJI lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 Raji cells were isolated from Burkitt lymphoma of the left maxilla in an 11-year-old black boy by Pulverleft RJV in 1963 and were the first continuous passage of the human hemopoietic system and the origin of B cells. This cell contains EBV and needs to be operated in a secondary biosafety cabinet; it can be used as a transfection host. Raji细胞由Pulvertaft RJV于1963年从一位11岁黑人男孩的左上颌骨的Burkitt淋巴瘤中分离建立的，是第一个人类造血系统的连续传代细胞，为B细胞起源。该细胞中含有EBV，需要在二级生物安全柜中操作；可作转染宿主。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 43~48 43~48 A类 Class A RAJI-Species Identification-PUMC RAJI-cell morphology-PUMC RAJI-物种鉴定-PUMC RAJI-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 4 � 105/ml ~ 3 � 106/ml; and replenish the fluid according to the cell concentration every 2 to 3 days. 维持细胞浓度在4×105/ml~3×106/ml；根据细胞浓度每2~3天补液1次。 NICR46 RAJI RAJI cell RAJI 细胞 PMID: 8557639 PMID: 8557639 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR46 cell NICR46 细胞 http://www.cellresource.cn/fdetail.aspx?id=46 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RAJI-Species Identification-PUMC RAJI-cell morphology-PUMC RAJI-物种鉴定-PUMC RAJI-细胞形态-PUMC 3111C0002000000025 Burkitt's Lymphoma Cells; Raji 黑人Burkitt淋巴瘤细胞；Raji lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 Raji cells were isolated from Burkitt lymphoma of the left maxilla in an 11-year-old black boy by Pulverleft RJV in 1963 and were the first continuous passage of the human hemopoietic system and the origin of B cells. This cell contains EBV and needs to be operated in a secondary biosafety cabinet; it can be used as a transfection host. Raji细胞由Pulvertaft RJV于1963年从一位11岁黑人男孩的左上颌骨的Burkitt淋巴瘤中分离建立的，是第一个人类造血系统的连续传代细胞，为B细胞起源。该细胞中含有EBV，需要在二级生物安全柜中操作；可作转染宿主。 RPMI 1640 (w/o Hepes) new-born calf serum，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Raji-cell morphology-NIFDC Raji-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 4 � 10 -5 cells/ml 4x10-5细胞/ml NICR397 Raji Raji cell Raji 细胞 NICR397 cell NICR397 细胞 http://www.cellresource.cn/fdetail.aspx?id=397 RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Raji-cell morphology-NIFDC Raji-细胞形态-NIFDC Amelogenin:X,Y；CSF1PO:10,12；D13S317:13；D16S539:8,11；D18S51:17；D19S433:14,14.2；D21S11:28,31；D2S1338:22；D3S1358:15,16；D5S818:10,13；D7S820:10；D8S1179:14,15；FGA:19,27；TH01:6,7；TPOX:8,13；vWA:17,19 Amelogenin:X,Y；CSF1PO:10,12；D13S317:13；D16S539:8,11；D18S51:17；D19S433:14,14.2；D21S11:28,31；D2S1338:22；D3S1358:15,16；D5S818:10,13；D7S820:10；D8S1179:14,15；FGA:19,27；TH01:6,7；TPOX:8,13；vWA:17,19 3131C0001000700044 Burkitt's Lymphoma Cells; RAJI 黑人Burkitt淋巴瘤细胞；RAJI lymphoblast 淋巴母细胞 suspension growth 悬浮生长 The lymphoblast-like Raji cell line originated from Burkitt's lymphoma of the left maxilla in an 11-year-old black boy. It is EBNA-positive. 淋巴母细胞样的Raji细胞株源自一位11岁黑人男孩的左上颌骨的Burkitt淋巴瘤。 EBNA阳性。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Raji-cell morphology-SHCRC Raji-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NICR614 RAJI RAJI cell RAJI 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR614 cell NICR614 细胞 http://www.cellresource.cn/fdetail.aspx?id=614 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Raji-cell morphology-SHCRC Raji-细胞形态-SHCRC 3131C0001000400018 Fetal Rhesus Monkey Kidney Cells; FRhK-4 [FRhK4] 恒河猴胚肾细胞；FRhK-4 [FRhK4] epithelial cell 上皮细胞 adherent growth 贴壁生长 FRhK-4 cell lines were derived from 300g kidney tissue of normal female embryos of rhesus monkey. This cell line is an epithelial cell-like continuous cell line and has been used by several laboratories for hepatitis A studies. FRhK-4细胞株来自300克重恒河猴正常雌性胚胎的肾组织。 该细胞系是上皮细胞样的连续细胞株，且已被数家实验室用作甲型肝炎研究。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P(30+5) P(30+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 4-5 days. 消化3-5分钟。1:2。4-5天可长满。 FRhK-4 FRhK-4 cell FRhK-4 细胞 FRhK4 FRhK4 cell FRhK4 细胞 NICR521 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR521 cell NICR521 细胞 http://www.cellresource.cn/fdetail.aspx?id=521 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3142C0001000000036 Fetal Rhesus Monkey Kidney Cells; FRhK-4 恒河猴胚肾细胞；FRhK-4 epithelial-like 上皮细胞样 adherent growth 贴壁生长 FRhK-4 cell lines were derived from 300g kidney tissue of normal female embryos of rhesus monkey. This cell line is an epithelial cell-like continuous cell line and has been used by several laboratories for hepatitis A studies. FRhK-4细胞株来自300克重恒河猴正常雌性胚胎的肾组织。该细胞系是上皮细胞样的连续细胞株，且已被数家实验室用作甲型肝炎研究。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C57 C57 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. FRhK-4 FRhK-4 cell FRhK-4 细胞 NICR804 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR804 cell NICR804 细胞 http://www.cellresource.cn/fdetail.aspx?id=804 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3111C0002000000078 Rhesus Monkey Kidney Cells; LLC-MK2 恒河猴肾细胞；LLC-MK2 adherent growth 贴壁生长 LLC-MK2 cell line is derived from renal cell suspensions from six adult green monkeys. The cell is reported to be sensitive to many viruses, including enteroviruses, rhinoviruses, orthomyxovirus and members of the poxvirus family. LLC-MK2细胞株源自六只成年绿猴的肾细胞悬液。据报道这个细胞对很多病毒敏感，包括肠道病毒、鼻病毒、粘病毒和痘病毒家族中的成员。 P292 P292 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A LLC-MK2-cell morphology-NIFDC LLC-MK2-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net LLC-MK2 LLC-MK2 cell LLC-MK2 细胞 NICR450 NICR450 cell NICR450 细胞 http://www.cellresource.cn/fdetail.aspx?id=450 LLC-MK2-cell morphology-NIFDC LLC-MK2-细胞形态-NIFDC 3131C0001000400012 Monkey Choroid- retinal Endothelial Cells; RF/6A 猴脉络膜-视网膜(内皮)细胞；RF/6A epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell line was transformed spontaneously in the early stage and has been passaged more than 540 times. Weibel-Palade bodies were observed under electron microscope. Higher generations of cells are used as trophoblast to enhance adherence, transit capacity, and to support melanocytes in strata pigmenti bulbi oculi. 这个细胞株在早期自发转化并已传代540次以上。 电镜下观察到Weibel-Palade颗粒。 高代次的细胞用作滋养层以增强贴壁，转接能力和供养眼色素层黑素细胞。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A LDH检测报告RF=6A-Isoenzyme-SHCRC LDH检测报告RF=6A-同工酶-SHCRC MDH检测报告RF=6A-Isoenzyme-SHCRC MDH检测报告RF=6A-同工酶-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR516 RF/6A RF/6A cell RF/6A 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR516 cell NICR516 细胞 http://www.cellresource.cn/fdetail.aspx?id=516 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf LDH检测报告RF=6A-Isoenzyme-SHCRC LDH检测报告RF=6A-同工酶-SHCRC MDH检测报告RF=6A-Isoenzyme-SHCRC MDH检测报告RF=6A-同工酶-SHCRC 3131C0001000900002 Chicken B Lymphoma Cells; DT40 鸡淋巴瘤细胞；DT40 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 DT40 was derived from the bursal lymphocyte strain of Hyline SC induced by avian leukosis virus. Primary lymphoma was obtained from one day old chickens infectied with Roche-associated virus 1 (RAV-1). The tumors generated in the bursa of Fabricius were made into cell suspension and then injected intravenously into receptor chickens of the same genotype. After one transplantation in vivo, DT40 cell line was established. The cells contains proviral genes that are integrated upstream of c-myc proto-oncogene and express high levels of c-myc RNA. It lacks a normal c-myc gene, but contains myc gene that are regulated by two copies of ALV. This cell line retains the ability to rearrange the immunoglobulin light chain gene (IgL). At the IgL site, DT40 contains a heavy chain. DT40是来自Hyline SC鸡法氏囊淋巴细胞株经鸟类白血病病毒诱导建株的。原始淋巴瘤用罗氏相关病毒1(RAV-1)感染出生１天的小鸡得到。 法氏囊中生成的肿瘤制成细胞悬液后通过静脉注射输入同基因型的受体小鸡。 经过一次体内移植后，建立了DT40细胞株。 这株细胞包含的前病毒基因整合在c-myc原癌基因的上游，表达的c-myc RNA水平较高。 它缺少一个正常c-myc基因，但含有两个拷贝ALV去调控的myc基因。 这株细胞保留了重排免疫球蛋白轻链基因(IgL)的能力。 在IgL位点，DT40包含一个重和一个胚系同源基因。 c-rel基因和v-rel癌基因在DT40细胞株中都能诱导组织相容性(MHC)II类抗原表达。 v-rel 诱导的MHCII表达比c-rel诱导快，且其有效性在数周后达到c-rel的50倍。 这株细胞呈淋巴母细胞表型。 传染性检测表明DT40释放低水平的传染性RAV-1。这株细胞可以用于稳转研究。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10%，chicken serum，5%；0.05mM β-mercaptoethanol DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%，鸡血清，5%；0.05mM β-巯基乙醇 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%，鸡血清，5%；0.05mM β-巯基乙醇 http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. DT40 DT40 cell DT40 细胞 NICR757 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR757 cell NICR757 细胞 http://www.cellresource.cn/fdetail.aspx?id=757 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%，鸡血清，5%；0.05mM β-巯基乙醇 URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%，鸡血清，5%；0.05mM β-巯基乙醇 URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3131C0001000400023 Embryonic Chicken Fibroblasts; UMNSAH/DF-1 鸡胚成纤维细胞；UMNSAH/DF-1 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 UMNSAH/DF-1 is a chicken cell line derived from 10-day-old ELL-0 eggs and turns to immortalized cells spontaneously. Primary chicken embryo fibroblasts were isolated and cultured in a culture medium; the cells were passaged until aging; the cells were centrifuged to keep cell culture at a coverage of 30% to 60% during aging; non-aging clones were identified and passaged at least 30 times. No clonal proliferation was observed on soft agar, suggesting that these cells were immortalized without transformation. This cell line can serve as a matrix for virus proliferation, recombinant protein expression and recombinant virus production. UMNSAH/DF-1是起源于10日龄的ELL-0鸡蛋的鸡细胞株，自发永生化。 分离原代鸡胚成纤维细胞并在培养液中培养；传代直到衰老；在衰老过程中离心以保持细胞培养在30%到60%满；不衰老的克隆进行鉴定并传代不少于30次。 在软琼脂上没有观察到克隆增殖，说明这些细胞是永生化而没有转化。 这株细胞可作为病毒增殖、重组蛋白表达和重组病毒生产的基质。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf 150 150 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B UMNSAH DF-1-cell morphology-SHCRC UMNSAH DF-1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR526 UMNSAH/DF-1 UMNSAH/DF-1 cell UMNSAH/DF-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR526 cell NICR526 细胞 http://www.cellresource.cn/fdetail.aspx?id=526 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf UMNSAH DF-1-cell morphology-SHCRC UMNSAH DF-1-细胞形态-SHCRC 3131C0001000400030 Embryonic Chicken Fibroblasts; UMNSAH/DF-1 鸡胚成纤维细胞；UMNSAH/DF-1 fibroblasts 成纤维细胞 adherent growth 贴壁生长 UMNSAH/DF-1 is a chicken cell line derived from 10-day-old ELL-0 eggs and turns to immortalized cells spontaneously. Primary chicken embryo fibroblasts were isolated and cultured in a culture medium; the cells were passaged until aging; the cells were centrifuged to keep cell culture at a coverage of 30% to 60% during aging; non-aging clones were identified and passaged at least 30 times. No clonal proliferation was observed on soft agar, suggesting that these cells were immortalized without transformation. This cell line can serve as a matrix for virus proliferation, recombinant protein expression and recombinant virus production. UMNSAH/DF-1是起源于10日龄的ELL-0鸡蛋的鸡细胞株，自发永生化。 分离原代鸡胚成纤维细胞并在培养液中培养；传代直到衰老；在衰老过程中离心以保持细胞培养在30%到60%满；不衰老的克隆进行鉴定并传代不少于30次。 在软琼脂上没有观察到克隆增殖，说明这些细胞是永生化而没有转化。 这株细胞可作为病毒增殖、重组蛋白表达和重组病毒生产的基质。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P(150+5) P(150+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A UMNSAH DF-1-cell morphology-SHCRC UMNSAH DF-1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1675 UMNSAH/DF-1 UMNSAH/DF-1 cell UMNSAH/DF-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1675 cell NICR1675 细胞 http://www.cellresource.cn/fdetail.aspx?id=1675 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf UMNSAH DF-1-cell morphology-SHCRC UMNSAH DF-1-细胞形态-SHCRC - - 3111C0001CCC000417 Embryonic Chicken Fibroblasts(Spontaneous Transformation); UMNSAH/DF1 鸡胚成纤维细胞（自发转化）；UMNSAH/DF1 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:6~1:8传代，2~3天传代一次 Passage at 1: 6 ~ 1: 8, and passage once in 2~3 days. NICR2036 UMNSAH/DF1 UMNSAH/DF1 cell UMNSAH/DF1 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2036 cell NICR2036 细胞 http://www.cellresource.cn/fdetail.aspx?id=2036 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3111C0001CCC000167 Acute T-lymphocytic Leukemia Cells; TALL-104 急性T淋巴细胞白血病细胞；TALL-104 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells are derived from the peripheral blood of a child with recurrent T-ALL (acute T-lymphoblastic leukemia), and have very strong cytotoxicity, and can destroy tumor cells both in vitro and vivo; IL-2 can promote the cell growth; the cells are α/β TCR-positive and γ/δ TCR negative; the cells can produce IFNγ, TNF-α and GM-CSF. 该细胞源于一名复发T-ALL（急性T淋巴细胞性白血病）的儿童的外周血；具有很强的细胞毒性，体内体外实验中都能破坏肿瘤细胞；IL-2可使细胞更好地生长；α/β TCR阳性，γ/δ TCR阴性；可产生IFNγ、TNF-α和GM-CSF。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 20%FBS IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 20%FBS http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell density between 4 � 105 ~ 1 � 106 cells/ml, and change fluid once in 2~3 days 维持细胞密度在4×105~1×106 cells/ml之间，2~3天换液1次　 NICR167 TALL-104 TALL-104 cell TALL-104 细胞 PMID: 1706955 PMID: 1706955 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR167 cell NICR167 细胞 http://www.cellresource.cn/fdetail.aspx?id=167 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf 3131C0001000700105 Acute Myeloid Leukemia Cells; KG-1 急性髓系细胞白血病细胞；KG-1 myeloblast 原粒细胞 suspension growth 悬浮生长 KG1 cell line was established by H.P. Koeffler and D. W. Golde. Bone marrow was drawn from a 59-year-old male white man with erythroleukemia which then developed into acute myeloid leukemia. KG1 cells are morphologically similar to acute myeloid leukemia and are characterized by pleomorphism, in which bone marrow mother cells and bone marrow cells are predominated. A few cells are mature granulocytes, and trace amounts of macrophages and eosinophils also exists. KG1细胞系由H.P.Koeffler和D.W.Golde建立。骨髓抽自一个59岁的患有红白血病而后又发展成急性骨髓原性白血病的男性白人。 KG1细胞在形态上类似急性髓原白血病，表现为明显的多形化，骨髓母细胞和骨髓细胞占优势。 少量细胞是成熟的粒细胞，也有微量的巨噬细胞和嗜曙红细胞。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. KG-1 KG-1 cell KG-1 细胞 NICR673 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR673 cell NICR673 细胞 http://www.cellresource.cn/fdetail.aspx?id=673 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3142C0001000000094 Acute Myeloid Leukemia Cells; KG-1 急性髓系细胞白血病细胞；KG-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 KG1 cell line was established by H.P. Koeffler and D. W. Golde. Bone marrow was drawn from a 59-year-old male white man with erythroleukemia which then developed into acute myeloid leukemia. KG1 cells are morphologically similar to acute myeloid leukemia and are characterized by pleomorphism, in which bone marrow mother cells and bone marrow cells are predominated. A few cells are mature granulocytes, and trace amounts of macrophages and eosinophils also exists. KG1细胞系由H.P.Koeffler和D.W.Golde建立。骨髓抽自一个59岁的患有红白血病而后又发展成急性骨髓原性白血病的男性白人。 KG1细胞在形态上类似急性髓原白血病，表现为明显的多形化，骨髓母细胞和骨髓细胞占优势。 少量细胞是成熟的粒细胞，也有微量的巨噬细胞和嗜曙红细胞。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C35 C35 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. KG-1 KG-1 cell KG-1 细胞 NICR862 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR862 cell NICR862 细胞 http://www.cellresource.cn/fdetail.aspx?id=862 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf - - 3111C0001CCC00X006 Anti-CEA Mouse Hybridoma; C1 抗CEA小鼠杂交瘤；C1 lymphoblast-like 淋巴母细胞样 semi-adherent growth 半贴壁生长 The cells can produce monoclonal antibodies and the antigen is carcinoembryonic antigen CEA. 可产生单克隆抗体，抗原为癌胚抗原CEA。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Biology Room, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所细胞生物室 69156981 Shiping Chen 69156981陈实平 Maintain the cell density in the 1 � 105 ~ 1 � 106 cells/ml, changing fluid for 2 to 3 times a week 保持细胞密度在1×105～1×106 cells/ml之间，每周换液2～3次 C1 C1 cell C1 细胞 NICR358 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR358 cell NICR358 细胞 http://www.cellresource.cn/fdetail.aspx?id=358 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf - - 3111C0001CCC00X007 Anti-CEA Mouse Hybridoma Cells; C2 抗CEA小鼠杂交瘤细胞；C2 lymphoblast-like 淋巴母细胞样 semi-adherent growth 半贴壁生长 The cells can produce monoclonal antibodies and the antigen is carcinoembryonic antigen CEA. 可产生单克隆抗体，抗原为癌胚抗原CEA。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Biology Room, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所细胞生物室 69156982 Shiping Chen 69156982陈实平 Maintain the cell density in the 1 � 105 ~ 1 � 106 cells/ml, changing fluid for 2 to 3 times a week 保持细胞密度在1×105～1×106 cells/ml之间，每周换液2～3次 C2 C2 cell C2 细胞 NICR359 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR359 cell NICR359 细胞 http://www.cellresource.cn/fdetail.aspx?id=359 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3142C0001000000020 Insect Ovarian Cells; Sf21 昆虫卵巢细胞；Sf21 round, multiform 圆形，多形 suspension growth 悬浮生长 The cell line originated from ovary tissue of pupae of the female Spodoptera frugiperda and is a clone line of Sf9 cell, which can be used for basic research of insect virus and replication of baculovirus expression vector. 该细胞系源于雌性草地贪夜蛾蛹的卵巢组织，是Sf9细胞的克隆系，可以用于昆虫病毒的基础研究和复制杆状病毒表达载体。 Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS http://www.cellresource.cn/pdf/20080108103149.pdf http://www.cellresource.cn/pdf/20080108103149.pdf C11 C11 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR788 Sf21 Sf21 cell Sf21 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR788 cell NICR788 细胞 http://www.cellresource.cn/fdetail.aspx?id=788 Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS URI: http://www.cellresource.cn/pdf/20080108103149.pdf Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS URI: http://www.cellresource.cn/pdf/20080108103149.pdf - - 3111C0001CCC000117 Insect Ovarian Cells; SF9 昆虫卵巢细胞；SF9 round 圆形 adherent/suspension growth 贴壁/悬浮生长 The cell line is derived from the ovarian tissue of the pupae of the female Spodoptera frugiperda and can be used to replicate baculovirus expression vectors. 该细胞系源于雌性草地贪夜蛾蛹的卵巢组织，可以用于复制杆状病毒表达载体。 Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS http://www.cellresource.cn/pdf/20080108103149.pdf http://www.cellresource.cn/pdf/20080108103149.pdf P36 P36 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:5或更多传代；2~3天换液1次。 Passage at 1: 5 or more; and change fluid once in 2~3 days. NICR117 SF9 SF9 cell SF9 细胞 PMID: 8631793 PMID: 8631793 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR117 cell NICR117 细胞 http://www.cellresource.cn/fdetail.aspx?id=117 Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS URI: http://www.cellresource.cn/pdf/20080108103149.pdf Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS URI: http://www.cellresource.cn/pdf/20080108103149.pdf 3142C0001000000008 Insect Ovarian Cells; SF9 昆虫卵巢细胞；SF9 round, multiform 圆形，多形 slight adherent/suspension growth 轻微贴壁/悬浮生长 The cell line is derived from the ovarian tissue of the pupae of the female Spodoptera frugiperda and can be used to replicate baculovirus expression vectors. 该细胞系源于雌性草地贪夜蛾蛹的卵巢组织，可以用于复制杆状病毒表达载体。  Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate)  10%FBS Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate)  10%FBS http://www.cellresource.cn/pdf/20080108103149.pdf http://www.cellresource.cn/pdf/20080108103149.pdf C32 C32 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A SF-9-cell morphology-CCTCC SF-9-细胞形态-CCTCC SF9-Myoplasma Detection-CCTCC SF9-支原体检测-CCTCC Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR776 SF9 SF9 cell SF9 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR776 cell NICR776 细胞 http://www.cellresource.cn/fdetail.aspx?id=776 Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate)  10%FBS URI: http://www.cellresource.cn/pdf/20080108103149.pdf Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate)  10%FBS URI: http://www.cellresource.cn/pdf/20080108103149.pdf SF-9-cell morphology-CCTCC SF-9-细胞形态-CCTCC SF9-Myoplasma Detection-CCTCC SF9-Myoplasma Detection-CCTCC SF9-支原体检测-CCTCC SF9-支原体检测-CCTCC 3142C0001000000319 Insect Cells; Hi-five(BTI-TN-5B1-4) 昆虫细胞；Hi-five(BTI-TN-5B1-4) lymphoblast-like 淋巴母细胞样 semi-adherent growth 半贴壁生长 The cells were deposited by Professor Hu Yuanyang, College of Life Sciences, Wuhan University. Hi-live (BTI-TN-5B1-4) cell is an insect cell, which is mainly used for the amplification of insect viruses and the study of the interaction between viruses and host cells, etc. 该细胞由武汉大学生命科学学院胡远扬教授提交保藏。Hi-five(BTI-TN-5B1-4)细胞是一株昆虫细胞，主要用于昆虫病毒的扩增、病毒与宿主细胞相互作用的研究等。 Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS http://www.cellresource.cn/pdf/20080108103149.pdf http://www.cellresource.cn/pdf/20080108103149.pdf C8 C8 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Hi-five(BTI-TN-5B1-4) Hi-five(BTI-TN-5B1-4) cell Hi-five(BTI-TN-5B1-4) 细胞 NICR1970 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1970 cell NICR1970 细胞 http://www.cellresource.cn/fdetail.aspx?id=1970 Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS URI: http://www.cellresource.cn/pdf/20080108103149.pdf Grace＇s Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 10%FBS URI: http://www.cellresource.cn/pdf/20080108103149.pdf 3142C0001000000041 Fetal Rhesus Monkey Kidney Cells; MA104 罗猴胎肾细胞；MA104 epithelial-like 上皮细胞样 adherent growth 贴壁生长 MA 104 cells are derived from rhesus monkey fetal kidney. The cells were highly sensitive to simian rotavirus SA11. The cells can be used for many virus proliferation. MA 104 细胞衍生于罗猴胎肾。细胞对猴轮状病毒 (Simian rotavirus SA11) 高度敏感。可用于很多病毒的增殖。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C11 C11 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. MA104 MA104 cell MA104 细胞 NICR809 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR809 cell NICR809 细胞 http://www.cellresource.cn/fdetail.aspx?id=809 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf AMEL:X,X；CSF1PO:9,10；D12S391:23,23；D13S317:8,8；D16S539:11,12；D18S51:15,16；D19S433:14,14.2；D21S11:29,31；D2S1338:17,17；D2S441:10,14；D3S1358:15,15；D5S818:11,12；D6S1043:11,11；D7S820:9,11；D8S1179:12,12；FGA:21,24；Penta D:9,13；Penta E:5,14；TH01:9.3,9.3；TPOX:8,9； AMEL:X,X；CSF1PO:9,10；D12S391:23,23；D13S317:8,8；D16S539:11,12；D18S51:15,16；D19S433:14,14.2；D21S11:29,31；D2S1338:17,17；D2S441:10,14；D3S1358:15,15；D5S818:11,12；D6S1043:11,11；D7S820:9,11；D8S1179:12,12；FGA:21,24；Penta D:9,13；Penta E:5,14；TH01:9.3,9.3；TPOX:8,9； 3142C0001000000965 Chronic Myeloid Leukemia Cells; K-562 慢性粒细胞白血病细胞；K-562 lymphoblastic 成淋巴细胞 suspension growth 悬浮生长 This cell is suitable for use as a transfected host, from pleural effusion of a 53-year-old female patient with chronic myeloid leukemia. 该细胞适合做转染宿主，来自于一位53岁女性慢性粒细胞白血病人的胸腔积液 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. K-562 K-562 cell K-562 细胞 NICR2202 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2202 cell NICR2202 细胞 http://www.cellresource.cn/fdetail.aspx?id=2202 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf STR Identification STR鉴定 3142C0001000000347 Feline Kidney Cells; CRFK 猫肾细胞；CRFK epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line was established by RA Van Deusen and was not infected with bovine viral diarrhea virus. 该细胞系由RA Van Deusen建立经检测，该细胞系未感染牛病毒性下痢病毒。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C10 C10 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. CRFK CRFK cell CRFK 细胞 NICR1998 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1998 cell NICR1998 细胞 http://www.cellresource.cn/fdetail.aspx?id=1998 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3111C0002000000047 Feline Kidney Cells; CRFK 猫肾细胞；CRFK epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell does not contain bovine viral diarrhea (BVD) virus. 此细胞不含牛病毒性痢疾(BVD)病毒。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) new-born calf serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P188 P188 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A CRFK-cell morphology-NIFDC CRFK-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1：2~1：8 Passage at 1: 2 ~ 1: 8 CRFK CRFK cell CRFK 细胞 NICR419 NICR419 cell NICR419 细胞 http://www.cellresource.cn/fdetail.aspx?id=419 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf CRFK-cell morphology-NIFDC CRFK-细胞形态-NIFDC 3131C0001000400016 Feline Kidney Cells; CRFK 猫肾细胞；CRFK epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell does not contain bovine viral diarrhea (BVD) virus. 此细胞不含牛病毒性痢疾(BVD)病毒。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 CRFK CRFK cell CRFK 细胞 NICR520 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR520 cell NICR520 细胞 http://www.cellresource.cn/fdetail.aspx?id=520 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000400005 Bovine Embryo Tracheal Cells; EBTr (NBL-4) 牛胚气管细胞；EBTr (NBL-4) fibroblasts 成纤维细胞 adherent growth 贴壁生长 It is bovine dysentery virus (BVDV)-negative. 牛痢疾病毒(BVDV)阴性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) fetal bovine serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A EBTR-cell morphology-SHCRC EBTR-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 EBTr (NBL-4) EBTr (NBL-4) cell EBTr (NBL-4) 细胞 NICR510 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR510 cell NICR510 细胞 http://www.cellresource.cn/fdetail.aspx?id=510 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf EBTR-cell morphology-SHCRC EBTR-细胞形态-SHCRC 3111C0002000000077 Bovine Turbinate Cells; BT 牛陀螺状细胞；BT adherent growth 贴壁生长 The cells are derived from newborn calves and cannot grow in a medium containing bovine serum. It can be used for culture and detection of bovine sensitive virus. 该细胞源自新生小牛，在含有牛源血清的培养基中不能生长。可用于牛敏感病毒的培养、检测。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%HS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%HS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P28 P28 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A BT-cell morphology-NIFDC BT-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. BT BT cell BT 细胞 NICR449 NICR449 cell NICR449 细胞 http://www.cellresource.cn/fdetail.aspx?id=449 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%HS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%HS URI: http://www.cellresource.cn/pdf/20080108100710.pdf BT-cell morphology-NIFDC BT-细胞形态-NIFDC - - 3111C0001CCC000078 Canine Kidney Cells; MDCK(NBL-2) 犬肾细胞；MDCK(NBL-2) epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 1958年，Madin SH和Darby NB从表观正常的成年雌性科克猎犬的肾建立了MDCK细胞株。角蛋白阳性。该细胞曾被用于研究β淀粉前体蛋白的形成及其蛋白水解产物的分类。 In 1958, S.H. Madin and N.B. Darby established MDCK cell lines from the kidneys of normal adult female Cocker Spaniel. The cells are positive for keratin by immunoperoxidase staining. The cells were used to study the formation of β-amyloid precursor proteins and the classification their proteolytic products. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 76~80 76~80 A类 Class A MDCK-Species Identification-PUMC MDCK-cell morphology-PUMC MDCK-物种鉴定-PUMC MDCK-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:6传代；2~3天换液1次。 Passage at 1: 2 ~ 1: 6; and change fluid once in 2~3 days. MDCK(NBL-2) MDCK(NBL-2) cell MDCK(NBL-2) 细胞 NICR78 PMID: 7860629 PMID: 7860629 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR78 cell NICR78 细胞 http://www.cellresource.cn/fdetail.aspx?id=78 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf MDCK-Species Identification-PUMC MDCK-cell morphology-PUMC MDCK-物种鉴定-PUMC MDCK-细胞形态-PUMC 3111C0002000000045 Canine Kidney Cells; MDCK(NBL-2) 犬肾细胞；MDCK(NBL-2) epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 1958年，Madin SH和Darby NB从表观正常的成年雌性科克猎犬的肾建立了MDCK细胞株。角蛋白阳性。该细胞曾被用于研究β淀粉前体蛋白的形成及其蛋白水解产物的分类。 In 1958, S.H. Madin and N.B. Darby established MDCK cell lines from the kidneys of normal adult female Cocker Spaniel. The cells are positive for keratin by immunoperoxidase staining. The cells were used to study the formation of β-amyloid precursor proteins and the classification their proteolytic products. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) new-born calf serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P63 P63 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A MDCK(NBL-2)-cell morphology-NIFDC MDCK(NBL-2)-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1：2~1：6 Passage at 1: 2 ~ 1: 6 MDCK(NBL-2) MDCK(NBL-2) cell MDCK(NBL-2) 细胞 NICR417 NICR417 cell NICR417 细胞 http://www.cellresource.cn/fdetail.aspx?id=417 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MDCK(NBL-2)-cell morphology-NIFDC MDCK(NBL-2)-细胞形态-NIFDC 3131C0001000400014 Canine Kidney Cells; MDCK(NBL-2) 犬肾细胞；MDCK(NBL-2) epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 1958年，Madin SH和Darby NB从表观正常的成年雌性科克猎犬的肾建立了MDCK细胞株。角蛋白阳性。该细胞曾被用于研究β淀粉前体蛋白的形成及其蛋白水解产物的分类。 In 1958, S.H. Madin and N.B. Darby established MDCK cell lines from the kidneys of normal adult female Cocker Spaniel. The cells are positive for keratin by immunoperoxidase staining. The cells were used to study the formation of β-amyloid precursor proteins and the classification their proteolytic products. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B MDCK-cell morphology-SHCRC MDCK-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2~1:6传代；2~3天换液1次。 Passage at 1: 2 ~ 1: 6; and change fluid once in 2~3 days. MDCK(NBL-2) MDCK(NBL-2) cell MDCK(NBL-2) 细胞 NICR518 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR518 cell NICR518 细胞 http://www.cellresource.cn/fdetail.aspx?id=518 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf MDCK-cell morphology-SHCRC MDCK-细胞形态-SHCRC 3131C0001000400024 Canine Kidney Cells; MDCK(NBL-2) 犬肾细胞；MDCK(NBL-2) epithelial cell 上皮细胞 adherent growth 贴壁生长 1958年九月 S.H. Madin and N.B. Darby从一只外观正常的成年雌性英国小猎犬的肾分离等到MDCK细胞株。角蛋白免疫过氧化物酶染色呈阳性。MDCK被用于研究β-粥样蛋白前体的处理及其蛋白水解产物。 In September, 1958, S.H. Madin and N.B. Darby Isolated the MDCK cell line from the kidney of a normal adult female English beagle. Immunoperoxidase staining for keratin is positive. MDCK is used to study the treatment of β-amyloid precursor proteins and their proteolytic products. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(55+5) P(55+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A MDCK-cell morphology-SHCRC MDCK-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 MDCK(NBL-2) MDCK(NBL-2) cell MDCK(NBL-2) 细胞 NICR527 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR527 cell NICR527 细胞 http://www.cellresource.cn/fdetail.aspx?id=527 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MDCK-cell morphology-SHCRC MDCK-细胞形态-SHCRC 3142C0001000000012 Canine Kidney Cells; MDCK(NBL-2) 犬肾细胞；MDCK(NBL-2) epithelial-like 上皮细胞样 adherent growth 贴壁生长 1958年，Madin SH和Darby NB从表观正常的成年雌性科克猎犬的肾建立了MDCK细胞株。角蛋白阳性。该细胞曾被用于研究β淀粉前体蛋白的形成及其蛋白水解产物的分类。 In 1958, S.H. Madin and N.B. Darby established MDCK cell lines from the kidneys of normal adult female Cocker Spaniel. The cells are positive for keratin by immunoperoxidase staining. The cells were used to study the formation of β-amyloid precursor proteins and the classification their proteolytic products. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C75 C75 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. MDCK(NBL-2) MDCK(NBL-2) cell MDCK(NBL-2) 细胞 NICR780 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR780 cell NICR780 细胞 http://www.cellresource.cn/fdetail.aspx?id=780 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3111C0002000000103 Canine Thymocytes; cf2Th 犬胸腺细胞；cf2Th adherent 贴壁 The cell is a sensitive strain to several retroviruses and can be used to amplify retroviruses. 该细胞为几种逆转录病毒的敏感株，可用于扩增逆转录病毒。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf 90%FBS+10%DMSO 90%FBS+10%DMSO Culture Method（-） 培养法（-） A类 Class A Cf2TH-cell morphology-NIFDC Cf2TH-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:2-1:6，每周换液2-3次 Passage at 1: 2-1: 6, and change fluid for 2-3 times a week NICR2127 cf2Th cf2Th cell cf2Th 细胞 NICR2127 cell NICR2127 细胞 http://www.cellresource.cn/fdetail.aspx?id=2127 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Cf2TH-cell morphology-NIFDC Cf2TH-细胞形态-NIFDC Amelogenin:X；CSF1PO:10,11；D13S317:11,12；D16S539:9；D18S51:15；D19S433:14,17.2；D21S11:27,28；D2S1338:17,21；D3S1358:16；D5S818:12,13；D7S820:11；D8S1179:13,15；FGA:22；TH01:7,9.3；TPOX:6,11；vWA:14 Amelogenin:X；CSF1PO:10,11；D13S317:11,12；D16S539:9；D18S51:15；D19S433:14,17.2；D21S11:27,28；D2S1338:17,21；D3S1358:16；D5S818:12,13；D7S820:11；D8S1179:13,15；FGA:22；TH01:7,9.3；TPOX:6,11；vWA:14 3131C0001000700075 Human Burkitt's Lymphoma Cells; NAMALWA 人Burkitt＇s淋巴瘤细胞；NAMALWA lymphoblast 淋巴母细胞 suspension growth 悬浮生长 This cell has the Epstein-Barr virus genome. 这株细胞有EB病毒基因组。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A NAMALWA-cell morphology-SHCRC NAMALWA-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NAMALWA NAMALWA cell NAMALWA 细胞 NICR643 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR643 cell NICR643 细胞 http://www.cellresource.cn/fdetail.aspx?id=643 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf NAMALWA-cell morphology-SHCRC NAMALWA-细胞形态-SHCRC 3142C0001000000083 Human Burkitt's Lymphoma Cells; CA46 人Burkitt淋巴瘤细胞；CA46 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 CA46 cell line originated from ascites fluid of patients with Burkitt's lymphoma and is one of the lymphoma cell lines established by Magrath. This cell line is negative for EBV nuclear antigen (EBNA) test, expressing surface IgM and secreting IgM which could not bind protein A. 12% of the cells population express complement receptor, but do not express Fc receptor. The population doubling time for cell growth was 16 hours, with a relatively fast growth rate. CA46细胞系源于Burkitt＇s淋巴瘤病人的腹水液，是Ｍａｇｒａｔｈ建立的系列淋巴瘤细胞系之一。该细胞系ＥＢＶ核抗原（ＥＢＮＡ）阴性，表达表面ＩｇＭ　和分泌不能与Ａ蛋白结合的ＩｇＭ，１２％的群体细胞表达补体受体，但不表达Ｆｃ受体。细胞生长的群体倍增时间是１６小时，具较快的生长速度。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C87 C87 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. CA46 CA46 cell CA46 细胞 NICR851 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR851 cell NICR851 细胞 http://www.cellresource.cn/fdetail.aspx?id=851 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：10，12；D13S317：8，12；D16S539：11，12；D18S51：13，16；D19S433：15.2；D21S11：28，29；D2S1338：20，22；D3S1358：16，18；D5S818：13；D7S820：11，12；D8S1179：15；FGA：19，23；TH01：7，9；TPOX：8，9；vWA：15，16； Amelogenin：X；CSF1PO：10，12；D13S317：8，12；D16S539：11，12；D18S51：13，16；D19S433：15.2；D21S11：28，29；D2S1338：20，22；D3S1358：16，18；D5S818：13；D7S820：11，12；D8S1179：15；FGA：19，23；TH01：7，9；TPOX：8，9；vWA：15，16； 3111C0001CCC000366 Human Burkitt's Lymphoma Cells; CA46 人burkitt淋巴瘤细胞；CA46 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells are derived from B lymphocytes from patients with Burkitt's lymphoma and were negative for EBNA and Fc detction. 该细胞源自Burkitt＇s淋巴瘤患者的B淋巴细胞，EBNA、Fc阴性。 RPMI 1640 (w/o Hepes) 20%FBS RPMI 1640 (w/o Hepes) 20%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C2 C2 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 46, XO, XY 46, XO, XY A类 Class A CA46-Species Identification-CRC CA46-物种鉴定-CRC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Cell density in the 2 ~ 3 � 105 cells/ml, saturation density of 2 � 106, changing fluid for 2 to 3 times a week 细胞密度在 2～3×105 cells/ml，饱和密度为2×106，每周换液2～3次 CA46 CA46 cell CA46 细胞 NICR1555 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1555 cell NICR1555 细胞 http://www.cellresource.cn/fdetail.aspx?id=1555 RPMI 1640 (w/o Hepes) 20%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 20%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf CA46-Species Identification-CRC CA46-物种鉴定-CRC Amelogenin:X,Y；CSF1PO:12；D13S317:11,12；D16S539:10,12；D18S51:16,18；D21S11:34,35；D3S1358:16,18；D5S818:8,13；D7S820:8,10；D8S1179:14；FGA:21,26；PentaD:10,12；PentaE:9；TH01:6,7；TPOX:8,11；vWA:15,17 Amelogenin:X,Y；CSF1PO:12；D13S317:11,12；D16S539:10,12；D18S51:16,18；D21S11:34,35；D3S1358:16,18；D5S818:8,13；D7S820:8,10；D8S1179:14；FGA:21,26；PentaD:10,12；PentaE:9；TH01:6,7；TPOX:8,11；vWA:15,17 3111C0002000000053 Human Burkitt's Lymphoma Cells; Daudi 人Burkitt淋巴瘤细胞；Daudi lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 1967年，该细胞系E. Klein 和 G. Klein建系，源于一名16岁患有Burkitt＇s淋巴瘤的黑人男性，beta-2-微球蛋白阴性，表达EBNA, VCA，sIg。该细胞携带EB病毒，是一个典型的B淋巴母细胞系，可用于引起白血病机制的研究。 In 1967, the cell line E. Klein and G. Klein was established from a 16-year-old black man with Burkitt's lymphoma, representing beta-2-microglobulin negative, expressing EBNA, VCA, and sIg. This cell carring Epstein - Barr virus (EBV), is a typical B-lymphoblastoid cell line, which can be used to the study of Induction mechanisms for leukemia. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Daudi-cell morphology-NIFDC Daudi-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net Maintain the cell concentration between 3 � 105 to 2 ~ 3 � 106 cells/ml, and change fluid once in 2~3 days. 维持细胞浓度在3×105至2~3×106 cells/ml之间，2~3天换液1次。 Daudi Daudi cell Daudi 细胞 NICR425 NICR425 cell NICR425 细胞 http://www.cellresource.cn/fdetail.aspx?id=425 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Daudi-cell morphology-NIFDC Daudi-细胞形态-NIFDC STR Identification STR鉴定 3142C0001000000082 Human Burkitt's Lymphoma Cells; Daudi 人Burkitt淋巴瘤细胞；Daudi lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 1967年，该细胞系E. Klein 和 G. Klein建系，源于一名16岁患有Burkitt＇s淋巴瘤的黑人男性，beta-2-微球蛋白阴性，表达EBNA, VCA，sIg。该细胞携带EB病毒，是一个典型的B淋巴母细胞系，可用于引起白血病机制的研究。 In 1967, the cell line E. Klein and G. Klein was established from a 16-year-old black man with Burkitt's lymphoma, representing beta-2-microglobulin negative, expressing EBNA, VCA, and sIg. This cell carring Epstein - Barr virus (EBV), is a typical B-lymphoblastoid cell line, which can be used to the study of Induction mechanisms for leukemia. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Daudi Daudi cell Daudi 细胞 NICR850 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR850 cell NICR850 细胞 http://www.cellresource.cn/fdetail.aspx?id=850 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X，Y；CSF1PO：12；D13S317：11，12；D16S539：10，12；D18S51：16，18；D19S433：12，14；D21S11：34，35；D2S1338：17，24；3S1358：16，18；D5S818：8，13；D7S820：8，10；D8S1179：14；FGA：21，26；TH01：6，7；TPOX：8，11；vWA：15，17； Amelogenin：X，Y；CSF1PO：12；D13S317：11，12；D16S539：10，12；D18S51：16，18；D19S433：12，14；D21S11：34，35；D2S1338：17，24；3S1358：16，18；D5S818：8，13；D7S820：8，10；D8S1179：14；FGA：21，26；TH01：6，7；TPOX：8，11；vWA：15，17； 3111C0001CCC000134 Human Burkkit's Lymphoma Cells; Daudi 人Burkkit淋巴瘤细胞；Daudi lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 1967年，该细胞系Klein E和Klein G建系，源于一名16岁患有Burkitt＇s淋巴瘤的黑人男性，beta-2-微球蛋白阴性，表达EBNA, VCA，sIg。该细胞携带EB病毒，是一个典型的B淋巴母细胞系，可用于白血病发病机制的研究。 In 1967, Klein E and Klein E was established from a 16-year-old black man with Burkitt's lymphoma, representing beta-2-microglobulin negative, expressing EBNA, VCA, and sIg. This cell carring Epstein - Barr virus (EBV), is a typical B-lymphoblastoid cell line, which can be used to the study of Induction mechanisms for leukemia. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 46，XY 46，XY A类 Class A Daudi-Cell Morphology-PUMC Daudi-Species Identification-PUMC Daudi-物种鉴定-PUMC Daudi-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 0.3 ~ 3 � 106 cells/ml, and change fluid once in 2~3 days. 维持细胞浓度在0.3~3×106 cells/ml之间，2~3天换液1次。 Daudi Daudi cell Daudi 细胞 NICR134 PMID: 8557337 PMID: 8557337 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR134 cell NICR134 细胞 http://www.cellresource.cn/fdetail.aspx?id=134 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Daudi-Cell Morphology-PUMC Daudi-Species Identification-PUMC Daudi-物种鉴定-PUMC Daudi-细胞形态-PUMC 3131C0001000700140 Human Burkkit's Lymphoma Cells; Daudi 人Burkkit淋巴瘤细胞；Daudi lymphoblast 淋巴母细胞 suspension growth 悬浮生长 1967年五月E. Klein and G. Klein从一位16岁黑人男性Burkitt＇s淋巴瘤患者建立了Daudi细胞株。 表面免疫球蛋白阳性(sIg+)。Β2-微球蛋白阴性，EBNA阳性，并有衣壳抗原VCA。细胞株携带EB病毒。 Daudi是典型的B淋巴母细胞，广泛应用于白血病发生机理的研究。 In May of 1967, E. Klein and G. Klein established the Daudi cell line from a 16-year-old black male Burkitt's lymphoma patient. The cells is surface immunoglobulin positive (sIg +). The cells B2-microglobulin negative, EBNA positive, with capsid antigen VCA. The cell line carries Epstein-Barr virus. Daudi is a typical B lymphoblast, which is widely used in the study of the pathogenesis mechanisms of leukemia. RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. Daudi Daudi cell Daudi 细胞 NICR708 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR708 cell NICR708 细胞 http://www.cellresource.cn/fdetail.aspx?id=708 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf STR Identification STR鉴定 3142C0001000000345 Human B Lymphoma Cells; Farage 人B淋巴瘤细胞；Farage lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 Farage细胞源于一名患有弥漫性大B细胞淋巴瘤(DLBCL）白人女性的活检淋巴组织，由H Ben-Bassat建系。经IL-4处理，该细胞CD21,CD22,CD54和CD58表达上调，而CD21, CD22, and CD38表达下调。 The Farage cells were derived from the biopsy lymphoid tissue of a white woman with diffuse large B-cell lymphoma (DLBCL), and established by H Ben-Bassat. After IL-4 treatment, the expression of CD21, CD22, CD54 and CD58 were up-regulated, while that of CD21, CD22 and CD38 was down-regulated. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C7 C7 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Farage Farage cell Farage 细胞 NICR1996 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1996 cell NICR1996 细胞 http://www.cellresource.cn/fdetail.aspx?id=1996 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000200021 Human B Lymphoblast Cells; HMy2.CIR 人B淋巴母细胞；HMy2.CIR lymphoblast 淋巴母细胞 suspension growth 悬浮生长 ARH-77细胞株的快速生长突变株HMy.2 B用γ射线照射，选择HLA I型抗原表达缺失的细胞，得到HMy2.CIR细胞株。 这株细胞不表达HLA A位点和B位点的产物，但表达少量HLA Cw4。 这株细胞适于用作I型主要组织相容性抗原基因的转染宿主。 有报道称ARH-77细胞株呈EB核抗原阳性(EBNA+)和EB病毒荚膜抗原阳性(EBVCA+)。 由于Hmy2.CIR细胞株起源于ARH-77细胞株的快速生长突变株HMy.2 B，推测它也是EBNA+。 The HMy2.CIR cell line was obtained from the rapid growth mutant HMy. 2B of ARH-77 cell line irradiated by γ-ray, in which the cells lacking the expression of HLA class I antigen were selected. This cell line did not express the products of HLA in site A and site B, but expressed a small amount of HLA Cw4. This cell strain is suitable for used as a transfected host for class I major histocompatibility complex gene. It is reported that ARH-77 cell line is positive in EB nuclear antigen (EBNA +) test and positive in EB virus capsule antigen (EBVCA +)test. Hmy2.CIR cell line originated from the fast-growing mutant HMy. 2 B of ARH-77 cell line, and It is also presumed that Hmy2.CIR cell line is positive in EB nuclear antigen (EBNA +) test. IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，10% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. HMy2.CIR HMy2.CIR cell HMy2.CIR 细胞 NICR479 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR479 cell NICR479 细胞 http://www.cellresource.cn/fdetail.aspx?id=479 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100822.pdf Amelogenin:X,Y；CSF1PO:11,12；D13S317:11；D16S539:11,12；D18S51:11,16；D21S11:29；D3S1358:16；D5S818:12,13；D7S820:9,11；D8S1179:10,13；FGA:22,24；PentaD:11,12；PentaE:5,7；TH01:8,9.3；TPOX:8,11；vWA:17,20 Amelogenin:X,Y；CSF1PO:11,12；D13S317:11；D16S539:11,12；D18S51:11,16；D21S11:29；D3S1358:16；D5S818:12,13；D7S820:9,11；D8S1179:10,13；FGA:22,24；PentaD:11,12；PentaE:5,7；TH01:8,9.3；TPOX:8,11；vWA:17,20 3111C0002000000032 Human B Lymphocytes; WIL2-S [WIL2S] 人B淋巴细胞；WIL2-S [WIL2S] lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells express no immunoglobulin and are HAT-sensitive. 不分泌免疫球蛋白,HAT敏感. RPMI 1640 (w/o Hepes) new-born calf serum，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 2x10-5 cells/ml 2x10-5细胞/ml NICR404 WIL2-S WIL2-S cell WIL2-S 细胞 WIL2S WIL2S cell WIL2S 细胞 NICR404 cell NICR404 细胞 http://www.cellresource.cn/fdetail.aspx?id=404 RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：XY；CSF1PO:10,12；D13S317:9,12；D16S539:9；D18S51:12,13；D19S433:13,15.2；D21S11:30；D2S1338:19,22；D3S1358:16；D5S818:10,13；D7S820:10,12；D8S1179:10,14；FGA:22,23；TH01:7,9；THOX:8,11；vWA:14,18； Amelogenin：XY；CSF1PO:10,12；D13S317:9,12；D16S539:9；D18S51:12,13；D19S433:13,15.2；D21S11:30；D2S1338:19,22；D3S1358:16；D5S818:10,13；D7S820:10,12；D8S1179:10,14；FGA:22,23；TH01:7,9；THOX:8,11；vWA:14,18； 3153C0001000000225 Human Leukemic B-cells; BALL-1 人B淋巴细胞白血病细胞；BALL-1 lymphoblastoid 淋巴细胞样 suspension growth 悬浮生长 The cell line is derived from a typical human B-lymphoblastic leukemia patient. 该细胞系来源于一典型的人B淋巴细胞白血病病人。 RPMI 1640（含2mM L-glutamine） (w/o Hepes) 10%FBS RPMI 1640（含2mM L-glutamine） (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN2 PN2 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） None 无 A类 Class A BALL-1-cell morphology-KCB BALL-1-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1:2传代；3-4天一次 Passage at 1: 2; and passage once in 3-4 days BALL-1 BALL-1 cell BALL-1 细胞 NICR2176 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2176 cell NICR2176 细胞 http://www.cellresource.cn/fdetail.aspx?id=2176 RPMI 1640（含2mM L-glutamine） (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640（含2mM L-glutamine） (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf BALL-1-cell morphology-KCB BALL-1-细胞形态-KCB 3111C0001CCC000047 Human Burkitt Lymphoma B Cells; RAMOS 人B淋巴细胞瘤细胞；RAMOS lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells are derived from a 3-year-old white Burkitt lymphoma patient and are EBV-negative; the cells express IL-4 receptor and low affinity IgE receptor (CD23); there are about 1500 IL-4 binding sites on each cell surface; the cells secrete IgM (lamda L) and express membrane and secretory immunoglobulin. 该细胞来源于一位3岁的白人Burkitt淋巴瘤患者；EBV阴性；表达IL-4受体和低亲和性IgE受体（CD23）；每个细胞表面大约有1500个IL-4的结合位点；分泌IgM（lamda L）；表达膜型和分泌型的免疫球蛋白。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 38~40 38~40 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 2 � 105/ml ~ 1 � 106/ml; and replenish the fluid according to the cell concentration every 2 to 3 days. 维持细胞浓度在2×105/ml~1×106/ml；根据细胞浓度每2~3天补液1次。 NICR47 RAMOS RAMOS cell RAMOS 细胞 PMID: 15562757 PMID: 15562757 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR47 cell NICR47 细胞 http://www.cellresource.cn/fdetail.aspx?id=47 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：10，11；D13S317：13，14；D16S539：10，13；D18S51：15；D6S1043：13,15；D21S11：30；D2S1338：20,23；D3S1358：14，15；D5S818：7,12；D7S820：11；D8S1179：13,16；FGA：20，24；Penta E：21；vWA：15,16； Amelogenin：X；CSF1PO：10，11；D13S317：13，14；D16S539：10，13；D18S51：15；D6S1043：13,15；D21S11：30；D2S1338：20,23；D3S1358：14，15；D5S818：7,12；D7S820：11；D8S1179：13,16；FGA：20，24；Penta E：21；vWA：15,16； 3111C0001CCC000048 Human Burkitt Lymphoma B Cells; RAMOS(RA.1) 人B淋巴细胞瘤细胞；RAMOS(RA.1) lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 The cells are derived from maternal Ramos and EBV-negative; The cells express IL-4 receptor and low affinity IgE receptor (CD23); there are about 1500 IL-4 binding sites on each cell surface; The cells secrete IgM(lamda L); The cells express membrane and secretory Immunoglobulin 该细胞来源于母系Ramos；EBV阴性；表达IL-4受体和低亲和性IgE受体（CD23）；每个细胞表面大约有1500个IL-4的结合位点；分泌IgM（lamda L）；表达膜型和分泌型的免疫球蛋白。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；3~5天1次。　 Passage at 1: 3; and passage once in 3 to 5 days. NICR48 RAMOS(RA.1) RAMOS(RA.1) cell RAMOS(RA.1) 细胞 PMID: 8943082 PMID: 8943082 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR48 cell NICR48 细胞 http://www.cellresource.cn/fdetail.aspx?id=48 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000200002 EB Virus-transformed Human B Cells; CGM1 人EB病毒转化的B细胞；CGM1 lymphoblastoid 淋巴细胞样 suspension growth 悬浮生长 EB转染B细胞 Epstein-Barr (EB) transfected B cells RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. CGM1 CGM1 cell CGM1 细胞 NICR462 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR462 cell NICR462 细胞 http://www.cellresource.cn/fdetail.aspx?id=462 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700190 Ph+ Acute Lymphoblastic Leukemia Cell Line; SUP-B15 人Ph+急淋白血病细胞系；SUP-B15 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 The cells came from the bone marrow of an 8-year-old child whose B cells were all Philadelphia chromosome-positive. These cells express multiple B cell markers but do not express T cell markers. Tests for Β-2 microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antibodies were positive. Tests for CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), My9 (CD33), surface antigen (sIg -), and Epstein-Barr virus were negative. 这株细胞来源于一位B细胞全部为费城染色体阳性的8岁小孩的骨髓。 这些细胞表达多个B细胞标记，但不表达T细胞标记。 β-2 微球蛋白，Leu12，My7 (CD13), OKT9 (CD71), OKT10 (CD38) 及 CALLA (CD10)抗体阳性。 CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), My9 (CD33), 表面抗原 (sIg -) IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，20% http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NICR1699 SUP-B15 SUP-B15 cell SUP-B15 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1699 cell NICR1699 细胞 http://www.cellresource.cn/fdetail.aspx?id=1699 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf 3142C0001000000031 Human T Lymphoma Cells; H9 人T淋巴瘤细胞；H9 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 H9 cells are clone lines (Callo, R C, et al) of HUT78 (ATCC TIB 161). The cell surface was labeled with CD3 and CD4. This cell line is sensitive to human immunodeficiency virus (HIV-1) and can be used to detect, isolate and proliferate HIV-1. It can also be used to study other human T cell viruses. H9细胞是HUT78（ATCC TIB 161）的克隆系（Callo，R C，et al）。细胞表面带有CD3、CD4标记。研究表明，该细胞系对人体免疫缺陷病毒（HIV-1）敏感，可用于检测、分离和增殖HIV-1，也可用于其它人类T cell病毒的研究。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C63 C63 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. H9 H9 cell H9 细胞 NICR799 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR799 cell NICR799 细胞 http://www.cellresource.cn/fdetail.aspx?id=799 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X,Y；CSF1PO:10；D13S317:8,11；D16S539:9,12；D18S51:12,13；D21S11:29,31.2；D3S1358:15,19；D5S818:13；D7S820:10,11；D8S1179:13,14；FGA:19,23；PentaD:10,13；PentaE:16,19,20；TH01:8,9；TPOX:8；vWA:17 Amelogenin:X,Y；CSF1PO:10；D13S317:8,11；D16S539:9,12；D18S51:12,13；D21S11:29,31.2；D3S1358:15,19；D5S818:13；D7S820:10,11；D8S1179:13,14；FGA:19,23；PentaD:10,13；PentaE:16,19,20；TH01:8,9；TPOX:8；vWA:17 3111C0002000000031 Human T Lymphoma Cells; HUT 102 人T淋巴瘤细胞；HUT 102 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 HTLV-1病毒阳性,IL-2可促进其生长 The cells is positive for HTLV-1 virus test , and IL-2 can promote the growth of the cells RPMI 1640 (w/o Hepes) new-born calf serum，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A HuT-102-cell morphology-NIFDC HuT-102-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 2 ~ 3x10-5 cells/ml 2~3x10-5细胞/ml HUT 102 HUT 102 cell HUT 102 细胞 NICR403 NICR403 cell NICR403 细胞 http://www.cellresource.cn/fdetail.aspx?id=403 RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf HuT-102-cell morphology-NIFDC HuT-102-细胞形态-NIFDC Amelogenin：X,Y；CSF1PO：8,11；D12S391：16,19；D13S317：11,13；D16S539：12,12；D18S51：20,20；D19S433：14,14.2；D21S11：28,29；D2S1338：19,23；D3S1358：15,16；D5S818：8,13；D6S1043：11,18；D7S820：8,10；D8S1179：13,15；FGA：24,24；Penta E：7,8；TH01：7,8；TPOX：6,6；vWA：16,19； Amelogenin：X,Y；CSF1PO：8,11；D12S391：16,19；D13S317：11,13；D16S539：12,12；D18S51：20,20；D19S433：14,14.2；D21S11：28,29；D2S1338：19,23；D3S1358：15,16；D5S818：8,13；D6S1043：11,18；D7S820：8,10；D8S1179：13,15；FGA：24,24；Penta E：7,8；TH01：7,8；TPOX：6,6；vWA：16,19； 3111C0001CCC000468 Human T Lymphoma Cells; HUT 102 人T淋巴瘤细胞；HUT 102 round; lymphoblast-like 圆形；淋巴母细胞样 suspension growth 悬浮生长 HUT 102 is a T cell lymphoma cell line. The cells were characterized by mature T cell lines, such as IL-2 activity in cell surface, and E-rosette positive, surface immunoglobulin-negative, EBV nuclear antigen-negative and TdT(Terminal deoxyribonucleotidyl-transferase)reaction-negative. This cell line can also release single retrovirus C types associated with T cell lymphoma, i.e., HTLVI virus. HUT 102是一株T细胞淋巴瘤细胞系。该细胞具有成熟T细胞系的特征，细胞表面呈现IL-2活性，E花环阳性，表面免疫球蛋白、EBV核抗原和TdT（Terminal deoxyribonucleotidyl-transferase）反应阴性。该细胞系还可释放与T细胞淋巴瘤相关的单一C型逆转病毒（retrovirus），即HTLVI病毒。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HUT 102 HUT 102 cell HUT 102 细胞 NICR2141 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2141 cell NICR2141 细胞 http://www.cellresource.cn/fdetail.aspx?id=2141 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf STR Identification STR鉴定 3142C0001000000051 Human T Lymphoma Cells; HUT 102 人T淋巴瘤细胞；HUT 102 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 HUT 102 is a T cell lymphoma cell line. The cells were characterized by mature T cell lines, such as IL-2 activity in cell surface, and E-rosette positive, surface immunoglobulin-negative, EBV nuclear antigen-negative and TdT(Terminal deoxyribonucleotidyl-transferase)reaction-negative. This cell line can also release single retrovirus C types associated with T cell lymphoma, i.e., HTLVI virus. HUT 102是一株T细胞淋巴瘤细胞系。该细胞具有成熟T细胞系的特征，细胞表面呈现IL-2活性，E花环阳性，表面免疫球蛋白、EBV核抗原和TdT（Terminal deoxyribonucleotidyl-transferase）反应阴性。该细胞系还可释放与T细胞淋巴瘤相关的单一C型逆转病毒（retrovirus），即HTLVI病毒。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HUT102 HUT102 cell HUT102 细胞 NICR819 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR819 cell NICR819 细胞 http://www.cellresource.cn/fdetail.aspx?id=819 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700096 Human T-lymphocyte Leukemia Cells; A3 人T淋巴细胞白血病细胞；A3 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 A3 subclone is derived from Jurkat cell line. Jurkat cells were treated with Fas antibody, and a cell line was obtained by limited dilution with low proportion of cells with spontaneous resistance induced by Fas-mediated apoptosis. The subcloning was very sensitive to Fas-mediated apoptosis. Wild type A3 cells resistant to neomycin were selected and treated with frameshift mutation mutagen ICR-191 for three times to isolate recessive mutants with anti-Fas antibody killing effect. A3亚克隆来自Jurkat细胞系。 用Fas抗体处理Jurkat细胞，用有限稀释法获得一个细胞系，此细胞系对Fas介导的凋亡产生自发抗性的比例较低。 得到的亚克隆对Fas-介导的凋亡十分敏感。 挑选对新霉素具有抗性的野生型A3细胞，并三次用移码突变诱变剂ICR-191进行处理以分离具有抗Fas抗体杀伤的隐性突变体。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. A3 A3 cell A3 细胞 NICR664 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR664 cell NICR664 细胞 http://www.cellresource.cn/fdetail.aspx?id=664 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X,Y；CSF1PO:10；D13S317:10,11；D16S539:12,13；D18S51:14,17；D21S11:27,30；D3S1358:15,16；D5S818:12；D7S820:9,10；D8S1179:11,14；FGA:21,22；PentaD:11,15；PentaE:7,11；TH01:6,9.3；TPOX:11；vWA:15,16 Amelogenin:X,Y；CSF1PO:10；D13S317:10,11；D16S539:12,13；D18S51:14,17；D21S11:27,30；D3S1358:15,16；D5S818:12；D7S820:9,10；D8S1179:11,14；FGA:21,22；PentaD:11,15；PentaE:7,11；TH01:6,9.3；TPOX:11；vWA:15,16 3111C0002000000027 Human T-lymphocyte Leukemia Cells; C8166 人T淋巴细胞白血病细胞；C8166 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 HTLV-1 infection HTLV-1感染 RPMI 1640 (w/o Hepes) new-born calf serum，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A C8166-cell morphology-NIFDC C8166-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 2 ~ 3x10-5 cells/ml 2~3x10-5细胞/ml C8166 C8166 cell C8166 细胞 NICR399 NICR399 cell NICR399 细胞 http://www.cellresource.cn/fdetail.aspx?id=399 RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf C8166-cell morphology-NIFDC C8166-细胞形态-NIFDC Amelogenin：X，Y；CSF1PO：11，12；D13S317：8，12；D16S539：11，12；D18S51：18；D19S433：14；D21S11：30；D2S1338：20，25；D3S1358：15，16；D5S818：11，12；D7S820：8，11；D8S1179：12，14；FGA：21，25；TH01：8，9；TPOX：8，9；vWA：14，15； Amelogenin：X，Y；CSF1PO：11，12；D13S317：8，12；D16S539：11，12；D18S51：18；D19S433：14；D21S11：30；D2S1338：20，25；D3S1358：15，16；D5S818：11，12；D7S820：8，11；D8S1179：12，14；FGA：21，25；TH01：8，9；TPOX：8，9；vWA：14，15； 3111C0001CCC000190 Human T-lymphocyte Leukemia Cells; HuT 78 人T淋巴细胞白血病细胞；HuT 78 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells can produce IL-2, TNF-α and express IL-2 receptor. 该细胞可产生IL-2，TNF-α；表达IL-2受体。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C2 C2 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 52~63 52~63 A类 Class A HuT 78-Species Identification-PUMC HuT 78-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 0.5 ~ 8 � 105 viable cells/ml; and change fluid for three times a week. 维持细胞浓度在0.5～8×105 viable cells/ml之间；每周换液3次。 HuT 78 HuT 78 cell HuT 78 细胞 NICR190 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR190 cell NICR190 细胞 http://www.cellresource.cn/fdetail.aspx?id=190 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf HuT 78-Species Identification-PUMC HuT 78-物种鉴定-PUMC Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,12；D16S539:11,12；D18S51:18；D19S433:14；D21S11:30；D2S1338:20,25；D3S1358:15,16；D5S818:11,12；D7S820:8,11；D8S1179:12,14；FGA:21,25；TH01:8,9；TPOX:8,9；vWA:14,15 Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,12；D16S539:11,12；D18S51:18；D19S433:14；D21S11:30；D2S1338:20,25；D3S1358:15,16；D5S818:11,12；D7S820:8,11；D8S1179:12,14；FGA:21,25；TH01:8,9；TPOX:8,9；vWA:14,15 3131C0001000700027 Human T-lymphocyte Leukemia Cells; HuT 78 人T淋巴细胞白血病细胞；HuT 78 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 The cells can produce IL-2, TNF-α and express IL-2 receptor. H9 (ATCC HTB-176) is a HuT 78 derived clone. 该细胞可产生IL-2，TNF-α；表达IL-2受体。H9 (ATCC HTB-176) 是HuT 78衍生出来的克隆。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium fetal bovine serum，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 胎牛血清，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 胎牛血清，20% http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. HuT 78 HuT 78 cell HuT 78 细胞 NICR597 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR597 cell NICR597 细胞 http://www.cellresource.cn/fdetail.aspx?id=597 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,12；D16S539:11,12；D18S51:18；D19S433:14；D21S11:30；D2S1338:20,25；D3S1358:15,16；D5S818:11,12；D7S820:8,11；D8S1179:12,14；FGA:21,25；TH01:8,9；TPOX:8,9；vWA:14,15 Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,12；D16S539:11,12；D18S51:18；D19S433:14；D21S11:30；D2S1338:20,25；D3S1358:15,16；D5S818:11,12；D7S820:8,11；D8S1179:12,14；FGA:21,25；TH01:8,9；TPOX:8,9；vWA:14,15 3131C0001001200004 Human T-lymphocyte Leukemia Cells; HuT 78 人T淋巴细胞白血病细胞；HuT 78 lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 The cells can produce IL-2, TNF-α and express IL-2 receptor. H9 (ATCC HTB-176) is a HuT 78 derived clone. 该细胞可产生IL-2，TNF-α；表达IL-2受体。H9 (ATCC HTB-176) 是HuT 78衍生出来的克隆。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium fetal bovine serum，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 胎牛血清，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 胎牛血清，20% http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. HuT 78 HuT 78 cell HuT 78 细胞 NICR1585 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1585 cell NICR1585 细胞 http://www.cellresource.cn/fdetail.aspx?id=1585 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,12；D16S539:11,12；D18S51:18；D19S433:14；D21S11:30；D2S1338:20,25；D3S1358:15,16；D5S818:11,12；D7S820:8,11；D8S1179:12,14；FGA:21,25；TH01:8,9；TPOX:8,9；vWA:14,15 Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,12；D16S539:11,12；D18S51:18；D19S433:14；D21S11:30；D2S1338:20,25；D3S1358:15,16；D5S818:11,12；D7S820:8,11；D8S1179:12,14；FGA:21,25；TH01:8,9；TPOX:8,9；vWA:14,15 3131C0001000700206 Human T-lymphocyte Leukemia Cells; HuT 78 人T淋巴细胞白血病细胞；HuT 78 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 The cells can produce IL-2, TNF-α and express IL-2 receptor. H9 (ATCC HTB-176) is a HuT 78 derived clone. 该细胞可产生IL-2，TNF-α；表达IL-2受体。H9 (ATCC HTB-176) 是HuT 78衍生出来的克隆。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，20% RPMI 1640 (w/o Hepes) 优质胎牛血清，20% RPMI 1640 (w/o Hepes) 优质胎牛血清，20% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. HuT 78 HuT 78 cell HuT 78 细胞 NICR1711 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1711 cell NICR1711 细胞 http://www.cellresource.cn/fdetail.aspx?id=1711 RPMI 1640 (w/o Hepes) 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X，Y；CSF1PO：12，15；D13S317：12，14；D16S539：10，12；D18S51：15，17；D19S433：12.2，16.2；D21S11：29；D2S1338：17，22；D3S1358：15，16；D5S818：9，10；D7S820：11；D8S1179：14；FGA：22；TH01：7；TPOX：6，9；vWA：17，19； Amelogenin：X，Y；CSF1PO：12，15；D13S317：12，14；D16S539：10，12；D18S51：15，17；D19S433：12.2，16.2；D21S11：29；D2S1338：17，22；D3S1358：15，16；D5S818：9，10；D7S820：11；D8S1179：14；FGA：22；TH01：7；TPOX：6，9；vWA：17，19； 3111C0001CCC000343 Human Burkitt's Lymphoma Cells; EB-3 人伯基特淋巴瘤细胞；EB-3 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells are derived from B lymphocytes of a 3-year-old black boy with Burkitt's lymphoma and are EBNA-positive. 该细胞源自一名3岁患有Burkitt＇s淋巴瘤的黑人男孩的B淋巴细胞，EBNA阳性。 RPMI 1640 (w/o Hepes) RPMI 1640 (w/o Hepes) http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P112 P112 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A EB-3-Cell Morphology-PUMC EB-3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell density of 3 � 105cells/ml, and change fluid for 2 to 3 times a week 保持细胞密度为3×105cells/ml，每周换液2～3次 EB-3 EB-3 cell EB-3 细胞 NICR1532 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1532 cell NICR1532 细胞 http://www.cellresource.cn/fdetail.aspx?id=1532 RPMI 1640 (w/o Hepes) URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) URI: http://www.cellresource.cn/pdf/20080108101040.pdf EB-3-Cell Morphology-PUMC EB-3-细胞形态-PUMC Amelogenin：X，Y；CSF1PO：10，12；D13S317：11；D16S539：11，12；D18S51：12，16；D19S433：13，14；D21S11：30；D2S1338：17，24；D3S1358：15，20；D5S818：11，12；D7S820：10，11.1；D8S1179：13；FGA：21，25；TH01：9.3；TPOX：8，11；vWA：16，19； Amelogenin：X，Y；CSF1PO：10，12；D13S317：11；D16S539：11，12；D18S51：12，16；D19S433：13，14；D21S11：30；D2S1338：17，24；D3S1358：15，20；D5S818：11，12；D7S820：10，11.1；D8S1179：13；FGA：21，25；TH01：9.3；TPOX：8，11；vWA：16，19； 3111C0001CCC000080 Human Osteosarcoma Cells; MG-63 人成骨肉瘤细胞；MG-63 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 The cells are derived from a 14-year-old white male with osteosarcoma; polyhypoxanthine nucleoside-polycytosine nucleotides, cycloheximide and actinomycin D can induce the production of high levels of interferon. The cells expressTGF-β receptor Ⅰ and Ⅱ. 该细胞源自14岁患有骨肉瘤的白人男性；聚次黄嘌呤核苷-聚胞嘧啶核苷酸、放线菌酮和放线菌素D可以诱导产生高水平的干扰素。该细胞表达TGF-β受体Ⅰ和Ⅱ。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A MG-63-Cell Morphology-PUMC MG-63-Species Identification-PUMC MG-63-cell morphology-PUMC MG-63-物种鉴定-PUMC MG-63-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代；每周换液2~3次。　 Passage at 1: 4 ~ 1: 8; and change fluid for 2~3 times a week. MG-63 MG-63 cell MG-63 细胞 NICR80 PMID: 883813 PMID: 883813 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR80 cell NICR80 细胞 http://www.cellresource.cn/fdetail.aspx?id=80 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf MG-63-Cell Morphology-PUMC MG-63-Species Identification-PUMC MG-63-cell morphology-PUMC MG-63-物种鉴定-PUMC MG-63-细胞形态-PUMC MG-63-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:10,12；D13S317:11；D16S539:11；D18S51:16；D19S433:13,14；D21S11:30；D2S1338:17,24；D3S1358:15；D5S818:11,12；D7S820:10；D8S1179:13；FGA:21,25；TH01:9.3；TPOX:8,11；vWA:16,19 Amelogenin:X,Y；CSF1PO:10,12；D13S317:11；D16S539:11；D18S51:16；D19S433:13,14；D21S11:30；D2S1338:17,24；D3S1358:15；D5S818:11,12；D7S820:10；D8S1179:13；FGA:21,25；TH01:9.3；TPOX:8,11；vWA:16,19 3131C0001000700124 Human Osteosarcoma Cells; MG-63 人成骨肉瘤细胞；MG-63 fibroblasts 成纤维细胞 adherent growth 贴壁生长 High levels of interferon production can be induced by polyhypoxanthine nucleoside-polycytosine nucleotides, cycloheximide and actinomycin D. 用聚次黄嘌呤核苷-聚胞嘧啶核苷酸，放线菌酮和放线菌素D可以诱导产生高水平的干扰素。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified heat-inactivatedfetal bovine serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A MG-63-cell morphology-SHCRC MG-63-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 MG-63 MG-63 cell MG-63 细胞 NICR692 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR692 cell NICR692 细胞 http://www.cellresource.cn/fdetail.aspx?id=692 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MG-63-cell morphology-SHCRC MG-63-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000073 Human Osteosarcoma Cells; MG-63 人成骨肉瘤细胞；MG-63 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from a 14-year-old white male with osteosarcoma; polyhypoxanthine nucleoside-polycytosine nucleotides, cycloheximide and actinomycin D can induce the production of high levels of interferon. The cells expressTGF-β receptor Ⅰ and Ⅱ. 该细胞源自14岁患有骨肉瘤的白人男性；聚次黄嘌呤核苷-聚胞嘧啶核苷酸、放线菌酮和放线菌素D可以诱导产生高水平的干扰素。该细胞表达TGF-β受体Ⅰ和Ⅱ。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C87 C87 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. MG-63 MG-63 cell MG-63 细胞 NICR841 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR841 cell NICR841 细胞 http://www.cellresource.cn/fdetail.aspx?id=841 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：10；D13S317：12，13；D16S539：12，13；D18S51：15；D19S433：13；D21S11：28，30；D2S1338：18；D3S1358：14，18；D5S818：12；D7S820：8，10；D8S1179：10，12；FGA：22，25；TH01：6，9；TPOX：8；vWA：18； Amelogenin：X；CSF1PO：10；D13S317：12，13；D16S539：12，13；D18S51：15；D19S433：13；D21S11：28，30；D2S1338：18；D3S1358：14，18；D5S818：12；D7S820：8，10；D8S1179：10，12；FGA：22，25；TH01：6，9；TPOX：8；vWA：18； 3111C0001CCC000025 Human Osteosarcoma Cells; Saos-2 人成骨肉瘤细胞；Saos-2 epithelium-like; polygonal shape 上皮样；多角形 adherent growth 贴壁生长 The cell is one of the numerous human tumor cell lines isolated and identified by Fogh J and Trempe G; the cell is from the osteosarcoma tissue of an 11-year-old white female. The patients were treated with radiotherapy and various drug therapies such as methotrexate, adriamycin, vincristine, cyclophosphamide and aramycin-C. The cells were not neoplastic in immunosuppressed mice and express epidermal growth factor EGF receptor, transforming growth factor β (type 1 and type 2) receptor. 该细胞是Fogh J和Trempe G分离和鉴定的众多人类肿瘤细胞系中的一种；该细胞来自一位11岁的白人女性的骨肉瘤组织。患者经过放疗以及氨甲喋呤、阿霉素、长春新碱、环磷酰胺和 aramycin-C等多种药物治疗。该细胞在免疫抑制小鼠中不致瘤，细胞表达表皮生长因子EGF受体、转化生长因子β（1型和2型）受体。 McCoy＇s 5A Media (Modified with Tricine) 15%FBS McCoy＇s 5A Media (Modified with Tricine) 15%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 46~88 46~88 A类 Class A Saos-2-Cell Morphology-PUMC Saos-2-Species Identification-PUMC Saos-2-物种鉴定-PUMC Saos-2-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；每周1~2次。　 Passage at 1: 2 ~ 1: 4; and passage for 1 ~ 2 times a week. NICR25 Saos-2 Saos-2 cell Saos-2 细胞 PMID: 8702614 PMID: 8702614 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR25 cell NICR25 细胞 http://www.cellresource.cn/fdetail.aspx?id=25 McCoy＇s 5A Media (Modified with Tricine) 15%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 15%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf Saos-2-Cell Morphology-PUMC Saos-2-Species Identification-PUMC Saos-2-物种鉴定-PUMC Saos-2-细胞形态-PUMC 3131C0001000700114 Human Osteosarcoma Cells; Saos-2 人成骨肉瘤细胞；Saos-2 epithelial cell 上皮细胞 adherent growth 贴壁生长 The cells were one of the numerous human tumor cell lines isolated and identified by Fogh and G. Trempe. The patient had been treated with multiple drugs, including RTG.methotrexate, adriamycin vincristine, cytoxan and aramyxin-C. The cells were not neoplastic in immunosuppressed mice, but formed colonies in semi-solid medium. 该细胞是J. Fogh和G.Trempe分离和鉴定的众多人肿瘤细胞系中的一种。该病人曾经多种药物治疗，包括RTG. methotrexate, adriamycin vincristine, cytoxan 和 aramycin-C。该细胞在免疫抑制小鼠中不致瘤，但在半固体培养基中形成集落。 McCoy＇s 5A Media (Modified with Tricine) qualified fetal bovine serum，15% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，15% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，15% http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P(28+5) P(28+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Saos-2-cell morphology-SHCRC Saos-2-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR682 Saos-2 Saos-2 cell Saos-2 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR682 cell NICR682 细胞 http://www.cellresource.cn/fdetail.aspx?id=682 McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，15% URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，15% URI: http://www.cellresource.cn/pdf/20080108100925.pdf Saos-2-cell morphology-SHCRC Saos-2-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000074 Human Osteosarcoma Cells; Saos-2 人成骨肉瘤细胞；Saos-2 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell is one of the numerous human tumor cell lines isolated and identified by Fogh J and Trempe G; the cell is from the osteosarcoma tissue of an 11-year-old white female. The patients were treated with radiotherapy and various drug therapies such as methotrexate, adriamycin, vincristine, cyclophosphamide and aramycin-C. The cells were not neoplastic in immunosuppressed mice and express epidermal growth factor EGF receptor, transforming growth factor β (type 1 and type 2) receptor. 该细胞是Fogh J和Trempe G分离和鉴定的众多人类肿瘤细胞系中的一种；该细胞来自一位11岁的白人女性的骨肉瘤组织。患者经过放疗以及氨甲喋呤、阿霉素、长春新碱、环磷酰胺和 aramycin-C等多种药物治疗。该细胞在免疫抑制小鼠中不致瘤，细胞表达表皮生长因子EGF受体、转化生长因子β（1型和2型）受体。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C29 C29 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR842 Saos-2 Saos-2 cell Saos-2 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR842 cell NICR842 细胞 http://www.cellresource.cn/fdetail.aspx?id=842 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf 3131C0001000700083 Human Megakaryocytic Leukemia Cells; MEG-01 人成巨核细胞白血病细胞；MEG-01 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 MEG-01 cell line is derived from bone marrow cells of a CML patient during megakaryocytic transformation. Tests for Cytoplasmic Factor VIII and surface globulin IIb/IIIa, Periodic acid-Schiff (PAS) reaction, α-naphthyl acetate esterase and acid phosphatase are positive. Tests for myeloperoxidase, α-naphthyl butyrate esterase, acetic acid AS-D naphthyl phenol esterase and alkaline phosphatase are negative. Staining with monoclonal antibodies BA-1 (anti-B cells, granulocyte), HPL-3 (anti-globulin IIb/IIIa) and 20.3 (anti-monocytes, platelets) was positive. Tests for other lymphoid and bone marrow antibodies are negative. MEG-01细胞株源自一位CML患者成巨核细胞转换期的骨髓细胞。 细胞质因子VIII和表面球蛋白IIb/IIIa，高碘酸-Schiff(PAS)反应，α醋酸萘酯酶和酸性磷酸酶阳性。 髓过氧化物酶，α丁酸萘酯酶，氯化醋酸AS-D萘苯酚酯酶和碱性磷酸酶阴性。 用单克隆抗体BA-1(抗B细胞，粒性白细胞)，HPL-3(抗球蛋白IIb/IIIa)和20.3(抗单核细胞，血小板)染色成阳性。 其他淋巴和骨髓类抗体成阴性。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf U U Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Positive 阳性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. MEG-01 MEG-01 cell MEG-01 细胞 NICR651 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR651 cell NICR651 细胞 http://www.cellresource.cn/fdetail.aspx?id=651 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700197 Human Megakaryocytic Leukemia Cells; MEG-01 人成巨核细胞白血病细胞；MEG-01 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 MEG-01 cell line is derived from bone marrow cells of a CML patient during megakaryocytic transformation. Tests for Cytoplasmic Factor VIII and surface globulin IIb/IIIa, Periodic acid-Schiff (PAS) reaction, α-naphthyl acetate esterase and acid phosphatase are positive. Tests for myeloperoxidase, α-naphthyl butyrate esterase, AS-D naphthyl phenol esterase and alkaline phosphatase are negative. Staining with monoclonal antibodies BA-1 (anti-B cells, granulocyte), HPL-3 (anti-globulin IIb/IIIa) and 20.3 (anti-monocytes, platelets) was positive. Tests for other lymphoid and bone marrow antibodies are negative. MEG-01细胞株源自一位CML患者成巨核细胞转换期的骨髓细胞。 细胞质因子VIII和表面球蛋白IIb/IIIa，高碘酸-Schiff(PAS)反应，α醋酸萘酯酶和酸性磷酸酶阳性。 髓过氧化物酶，α丁酸萘酯酶，氯化醋酸AS-D萘苯酚酯酶和碱性磷酸酶阴性。 用单克隆抗体BA-1(抗B细胞，粒性白细胞)，HPL-3(抗球蛋白IIb/IIIa)和20.3(抗单核细胞，血小板)染色成阳性。 其他淋巴和骨髓类抗体成阴性。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. MEG-01 MEG-01 cell MEG-01 细胞 NICR2095 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2095 cell NICR2095 细胞 http://www.cellresource.cn/fdetail.aspx?id=2095 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X,Y；CSF1PO：10,10；D12S391：19,19；D13S317：8,8；D16S539：9,9；D18S51：18,22；D19S433：14,15.2；D21S11：29,29；D2S1338：19,19；D3S1358：15,15；D5S818：13,13；D6S1043：14,18；D7S820：11,11；D8S1179：14,15；FGA：26,26；Penta E：15,15；TH01：7,7；TPOX：8,11；vWA：16,16； Amelogenin：X,Y；CSF1PO：10,10；D12S391：19,19；D13S317：8,8；D16S539：9,9；D18S51：18,22；D19S433：14,15.2；D21S11：29,29；D2S1338：19,19；D3S1358：15,15；D5S818：13,13；D6S1043：14,18；D7S820：11,11；D8S1179：14,15；FGA：26,26；Penta E：15,15；TH01：7,7；TPOX：8,11；vWA：16,16； 3111C0001CCC000521 Human Megakaryocytic Leukemia Cells; MEG-01 人成巨核细胞白血病细胞；MEG-01 round 圆形 suspension growth 悬浮生长 RPMI 1640 (w/o Hepes) 20%FBS RPMI 1640 (w/o Hepes) 20%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com MEG-01 MEG-01 cell MEG-01 细胞 NICR2442 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2442 cell NICR2442 细胞 http://www.cellresource.cn/fdetail.aspx?id=2442 RPMI 1640 (w/o Hepes) 20%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 20%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf AMEL:X,X；CSF1PO:11,11；D12S391:18,22；D13S317:11,11；D16S539:8,13；D18S51:13,16；D19S433:13,14；D21S11:31,31.2；D2S1338:17,19；D2S441:11,11.3；D3S1358:15,16；D5S818:12,12；D6S1043:12,18；D7S820:7,10；D8S1179:15,15；FGA:23.2,24；Penta D:10,12；Penta E:7,11；TH01:7,10；TPOX: AMEL:X,X；CSF1PO:11,11；D12S391:18,22；D13S317:11,11；D16S539:8,13；D18S51:13,16；D19S433:13,14；D21S11:31,31.2；D2S1338:17,19；D2S441:11,11.3；D3S1358:15,16；D5S818:12,12；D6S1043:12,18；D7S820:7,10；D8S1179:15,15；FGA:23.2,24；Penta D:10,12；Penta E:7,11；TH01:7,10；TPOX: 3142C0001000000964 Human Neuroblastoma (from Bone Marrow); SH-SY5Y 人成神经细胞瘤 （来自骨髓）；SH-SY5Y epithelial-like 上皮细胞样 mixed of adherent growth and suspension growth 贴壁生长与悬浮生长混合型 This cell line was established in 1978 and was derived from metastatic bone tumors, 该细胞系建立于1978年，来自于转移性骨肿瘤 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR2201 SH-SY5Y SH-SY5Y cell SH-SY5Y 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2201 cell NICR2201 细胞 http://www.cellresource.cn/fdetail.aspx?id=2201 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin:X,Y；CSF1PO:10；D13S317:11；D16S539:12；D18S51:11；D19S433:13,14；D21S11:31,33.2；D2S1338:17；D3S1358:15,16；D5S818:11；D7S820:8,10；D8S1179:10；FGA:20；TH01:8；TPOX:8；vWA:17 Amelogenin:X,Y；CSF1PO:10；D13S317:11；D16S539:12；D18S51:11；D19S433:13,14；D21S11:31,33.2；D2S1338:17；D3S1358:15,16；D5S818:11；D7S820:8,10；D8S1179:10；FGA:20；TH01:8；TPOX:8；vWA:17 3131C0001000700121 Cell Lung Cancer Cells; NCI-H661 人大细胞肺癌细胞；NCI-H661 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell line is derived from lymph nodes of a 43-year-old white male with large cell lung cancer. There is a lack of submicroscopic structure and biochemical evidence for mucus and squamous differentiation. P53 mRNA expression is easy to detect, which is significantly higher than the level of normal lung cells; at the same time, there is no general structural DNA alteration, which can indicate the presence of point mutations or small variations. Detections for keratin and vimentin fibers are positive and detection for neurofilament triplet protein is negative. 此细胞株源自一43岁患有大细胞肺癌的男性白人的淋巴结。 缺乏产生粘液和鳞状分化的亚显微结构和生化证据。 其表达的p53 mRNA易于检测，明显高于正常肺细胞的水平，与此同时，没有出现总体性的结构DNA崎变，它们可以说明存在点突变或很小的变异。 角蛋白和波形蛋白纤维染色阳性，神经丝三联体蛋白阴性。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A NCI-H661-DNA-Fingerprinting-SHCRC NCI-H661-DNA-指纹-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NCI-H661 NCI-H661 cell NCI-H661 细胞 NICR689 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR689 cell NICR689 细胞 http://www.cellresource.cn/fdetail.aspx?id=689 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H661-DNA-Fingerprinting-SHCRC NCI-H661-DNA-指纹-SHCRC Amelogenin：X，Y；CSF1PO：10；D13S317：11；D16S539：12；D18S51：11；D19S433：13，14；D21S11：31，33.2；D2S1338：17；D3S1358：16；D5S818：11；D7S820：8，10；D8S1179：10；FGA：20；TH01：8；TPOX：8；vWA：17； Amelogenin：X，Y；CSF1PO：10；D13S317：11；D16S539：12；D18S51：11；D19S433：13，14；D21S11：31，33.2；D2S1338：17；D3S1358：16；D5S818：11；D7S820：8，10；D8S1179：10；FGA：20；TH01：8；TPOX：8；vWA：17； 3111C0001CCC000357 Cell Lung Cancer Cells; NCI-H661 人大细胞肺癌细胞；NCI-H661 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell line was established in 1982 and originated from the pleural effusion of a man with large cell lung cancer. Detections for cytokeratin and vimentin are positive. 该细胞1982年建系，源自一位患有大细胞肺癌的男性的胸腔积液。该细胞角蛋白、波形蛋白阳性。 RPMI 1640 (w/o Hepes) 10% FBS RPMI 1640 (w/o Hepes) 10% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P46 P46 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 130～153 130～153 A类 Class A NCI-H661-Cell Morphology-PUMC NCI-H661-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3～1:5传代，每周换液2～3次 Passage at 1: 3 ~ 1: 5, and change fluid for 2 to 3 times a week NCI-H661 NCI-H661 cell NCI-H661 细胞 NICR1546 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1546 cell NICR1546 细胞 http://www.cellresource.cn/fdetail.aspx?id=1546 RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H661-Cell Morphology-PUMC NCI-H661-细胞形态-PUMC Amelogenin：X；CSF1PO：9，10；D13S317：12，13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：21；TH01：7；TPOX：8，12；vWA：16，18； Amelogenin：X；CSF1PO：9，10；D13S317：12，13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：21；TH01：7；TPOX：8，12；vWA：16，18；　 3111C0001CCC000067 Low Differentiation Human Lung Adenocarcinoma Cells; GLC-82 [GLC82] 人低分化肺腺癌细胞；GLC-82 [GLC82] epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from a 58-year-old Chinese woman with lung adenocarcinoma; the primary cultured cell began to grow on the 7th day, and was first passage after 84 days and cultured for 24 months; the cells were polygonal; immunosuppressed Kunming mice and Wistar rats were inoculated subcutaneously successfully. STR detection revealed that the cells were contaminated by HeLa cells. 该细胞源自一位患有肺腺癌的58岁的中国女性；原代培养，7天细胞开始生长，首次传代84天，培养24个月；该细胞呈多边形；免疫抑制昆明小鼠及Wistar断奶大鼠癌细胞悬液皮下接种成功。STR检测发现该细胞被HeLa细胞污染。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 62~64 62~64 A类 Class A GLC-82-Species Identification-PUMC GLC-82-cell morphology-PUMC GLC-82-物种鉴定-PUMC GLC-82-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:5~1:10传代，建议初始浓度为 2~4×104 cells/cm2；2~3天换液1次。　 Passage at 1: 5 ~ 1:10, the proposed initial concentration is 2 ~ 4 � 104 cells/cm2; and change fluid once in 2~3 days. GLC-82 GLC-82 cell GLC-82 细胞 GLC82 GLC82 cell GLC82 细胞 NICR67 张咏, and 王阁. "重组人肝细胞生成素的生物学性能." 生理学报 51.3(1999):347-350. 张咏, and 王阁. "重组人肝细胞生成素的生物学性能." 生理学报 51.3(1999):347-350. 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR67 cell NICR67 细胞 http://www.cellresource.cn/fdetail.aspx?id=67 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf GLC-82-Species Identification-PUMC GLC-82-cell morphology-PUMC GLC-82-物种鉴定-PUMC GLC-82-细胞形态-PUMC Amelogenin：X；CSF1PO：10；D13S317：10；D16S539：8；D18S51：18；D19S433：15；D21S11：29，30，30.2；D2S1338：16，17；D3S1358：18；D5S818：11；D7S820：9；D8S1179：10；FGA：21，22；TH01：7；TPOX：8，10；vWA：16，17； Amelogenin：X；CSF1PO：10；D13S317：10；D16S539：8；D18S51：18；D19S433：15；D21S11：29，30，30.2；D2S1338：16，17；D3S1358：18；D5S818：11；D7S820：9；D8S1179：10；FGA：21，22；TH01：7；TPOX：8，10；vWA：16，17； 3111C0001CCC000237 Low Differentiation Human Lung Adenocarcinoma Cells; SK-LU-1 人低分化肺腺癌细胞；SK-LU-1 epithelioid 上皮样 adherent growth 贴壁生长 The cell line is derived from lung adenocarcinoma tissue from a 60 -year-old white female patient. 该细胞系源于一位60岁的白人女性患者的肺腺癌组织。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA, 1mM sodium pyruvate MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA, 1mM sodium pyruvate http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P58 P58 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代；每周换液2次。 Passage at 1: 2; and change fluid for 2 times a week. NICR237 SK-LU-1 SK-LU-1 cell SK-LU-1 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR237 cell NICR237 细胞 http://www.cellresource.cn/fdetail.aspx?id=237 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA, 1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA, 1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000700154 Human Small Cell Lung Cancer Cells; NCI-H1688 人典型小细胞肺癌细胞；NCI-H1688 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from liver metastases in a patient before treatment. 这株细胞来源于一位治疗之前的患者的肝转移灶。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(42+5) P(42+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NCI-H1688 NCI-H1688 cell NCI-H1688 细胞 NICR720 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR720 cell NICR720 细胞 http://www.cellresource.cn/fdetail.aspx?id=720 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X，Y；CSF1PO：12；D13S317：11；D16S539：9；D18S51：15，19；D19S433：13.2，14；D21S11：28，29；D2S1338：20；D3S1358：16，17；D5S818：11，13；D7S820：9，10；D8S1179：12，13；FGA：19；TH01：8；TPOX：8，11；vWA：16，18； Amelogenin：X，Y；CSF1PO：12；D13S317：11；D16S539：9；D18S51：15，19；D19S433：13.2，14；D21S11：28，29；D2S1338：20；D3S1358：16，17；D5S818：11，13；D7S820：9，10；D8S1179：12，13；FGA：19；TH01：8；TPOX：8，11；vWA：16，18； 3111C0001CCC000083 Human Multiple Myeloma Cells; RPMI-8226 [RPMI8826] 人多发性骨髓瘤细胞；RPMI-8226 [RPMI8826] lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 It is derived from the plasmacytoma of a 61-year-old male and it can produce an immunoglobulin light chain, with no heavy chain detected. 来源于一位61岁的男性浆细胞瘤患者；可产生免疫球蛋白轻链，未检测到重链。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 64~72，XY 64~72，XY A类 Class A RPMI-8226-Species Identification-PUMC RPMI-8226-cell morphology-PUMC RPMI-8226-物种鉴定-PUMC RPMI-8226-细胞形态-PUMC RPMI8226-Cell Morphology-PUMC RPMI8226-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；5～7天1次。　 Passage at 1: 3; and passage once in 5 to 7 days. NICR83 RPMI-8226 RPMI-8226 cell RPMI-8226 细胞 RPMI8826 RPMI8826 cell RPMI8826 细胞 PMID: 16113057 PMID: 16113057 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR83 cell NICR83 细胞 http://www.cellresource.cn/fdetail.aspx?id=83 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI-8226-Species Identification-PUMC RPMI-8226-cell morphology-PUMC RPMI-8226-物种鉴定-PUMC RPMI-8226-细胞形态-PUMC RPMI8226-Cell Morphology-PUMC RPMI8226-细胞形态-PUMC Amelogenin：X，Y；CSF1PO：10，12；D13S317：13；D16S539：11，12，13；D18S51：14；D19S433：14，15.2；D21S11：30，31；D2S1338：22，25；D3S1358：16；D5S818：9，12；D7S820：10，12；D8S1179：13，15；FGA：23；TH01：9.3；TPOX：8；vWA：18，19； Amelogenin：X，Y；CSF1PO：10，12；D13S317：13；D16S539：11，12，13；D18S51：14；D19S433：14，15.2；D21S11：30，31；D2S1338：22，25；D3S1358：16；D5S818：9，12；D7S820：10，12；D8S1179：13，15；FGA：23；TH01：9.3；TPOX：8；vWA：18，19； 3111C0001CCC000019 Human Malignant Multiple Teratoma Cells; NTERA-2 人恶性多发性畸胎瘤细胞；NTERA-2 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from nude mouse xenografts of the Tera-2 cell line in 1980. 该细胞是1980年从Tera-2细胞系的裸鼠异种移植瘤中分离建立的。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 62~67 62~67 A类 Class A NTERA-2-Species Identification-PUMC NTERA-2-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com The cells need to be maintained at a high density when inoculated with at least 5 x 106 cells in a T75 flask. Passage once every 2 to 3 days. 该细胞培养时需要维持较高的密度，T75瓶中至少接种5×106个细胞。每2~3天传代1次。 NICR19 NTERA-2 NTERA-2 cell NTERA-2 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR19 cell NICR19 细胞 http://www.cellresource.cn/fdetail.aspx?id=19 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf NTERA-2-Species Identification-PUMC NTERA-2-物种鉴定-PUMC 3131C0001000200020 Natural Killer Cells in Patients with Malignant Non-hodgkin Lymphoma; NK-92 [NK92] 人恶性非霍奇金淋巴瘤患者的自然杀伤细胞；NK-92 [NK92] lymphoblast 淋巴母细胞 suspension growth 悬浮生长 NK-92 is an interleukin-2 dependent NK cell line derived from peripheral blood monocytes of a 50-year-old white male with acute non-Hodgkin's lymphoma. This cell is cytotoxic to many malignant cells; chromium release tests show that it can kill K562 and Daudi cells. NK-92 cells (irradiated to prevent proliferation) can be effectively used for in vitro immune dissection of leukemia in blood without compromising the function of blood cells. NK-92 cells have the following characteristics: surface markers of CD2, CD7, CD11a, CD28, CD45 is positive, CD56 bright; surface markers of CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR is negative. NK-92是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株白细胞介素-2依赖型NK细胞株。 这株细胞对很多恶性细胞有细胞毒性；铬释放试验显示它能杀死K562和Daudi细胞。 NK-92细胞(经过照射以防止增殖)可以有效地用于血液中白血病的体外免疫清扫而不危及血细胞的功能。 NK-92细胞有以下特征： CD2, CD7, CD11a, CD28, CD45, CD54表面标记阳性，CD56亮；CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34和HLA-DR表面标记阴性。 http://www.cellresource.cn/pdf/20080108100503.pdf http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) qualified fetal bovine serum，10% α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NICR478 NK-92 NK-92 cell NK-92 细胞 NK92 NK92 cell NK92 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR478 cell NICR478 细胞 http://www.cellresource.cn/fdetail.aspx?id=478 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100503.pdf Amelogenin:X,Y；CSF1PO:11,12；D13S317:9,12；D16S539:11,12；D18S51:12,17；D21S11:31.2,32；D3S1358:15；D5S818:12,13；D7S820:10,11；D8S1179:12,15；FGA:20,22；PentaD:10,12；PentaE:12；TH01:6,9.3；TPOX:8；vWA:16,18 Amelogenin:X,Y；CSF1PO:11,12；D13S317:9,12；D16S539:11,12；D18S51:12,17；D21S11:31.2,32；D3S1358:15；D5S818:12,13；D7S820:10,11；D8S1179:12,15；FGA:20,22；PentaD:10,12；PentaE:12；TH01:6,9.3；TPOX:8；vWA:16,18 3111C0002000000065 Natural Killer Cells in Patients with Malignant Non-hodgkin Lymphoma; NK-92MI [NK92MI] 人恶性非霍奇金淋巴瘤患者的自然杀伤细胞；NK-92MI [NK92MI] lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 NK-92 is an interleukin-2 dependent NK cell line derived from peripheral blood monocytes of a 50-year-old white male with acute non-Hodgkin's lymphoma. NK-92MI is an IL-2 independent NK cell line derived from NK-92 transfected cells. The parent cells were transformed with human IL-2 cDNA carried by the retroviral MFG-hIL-2 vector used in microsomal gene transformation.NK-92MI is an IL-2 independent NK cell line derived from NK-92 transfected cells. The parent cells were transformed with human IL-2 cDNA carried by the retroviral MFG-hIL-2 vector used in microsomal gene transformation. Possibly due to the integration of the vector into the genomic DNA, the transformation is stable. This cell is cytotoxic to many malignant cells; chromium release tests show that it can kill K562 and Daudi cells. NK-92 cells have the following characteristics: surface markers of CD2, CD7, CD11a, CD28, CD45 is positive, CD56 bright; surface markers of CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR is negative. NK-92是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株白细胞介素-2依赖型NK细胞株。 这株细胞对很多恶性细胞有细胞毒性；铬释放试验显示它能杀死K562和Daudi细胞。 NK-92细胞(经过照射以防止增殖)可以有效地用于血液中白血病的体外免疫清扫而不危及血细胞的功能。 NK-92细胞有以下特征： CD2, CD7, CD11a, CD28, CD45, CD54表面标记阳性，CD56亮；CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34和HLA-DR表面标记阴性。 http://www.cellresource.cn/pdf/20080108100503.pdf http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) horse serum， 12.5% qualified fetal bovine serum，120.005 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A NK-92MI-cell morphology-NIFDC NK-92MI-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NICR437 NK-92MI NK-92MI cell NK-92MI 细胞 NK92MI NK92MI cell NK92MI 细胞 NICR437 cell NICR437 细胞 http://www.cellresource.cn/fdetail.aspx?id=437 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 URI: http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 URI: http://www.cellresource.cn/pdf/20080108100503.pdf NK-92MI-cell morphology-NIFDC NK-92MI-细胞形态-NIFDC 3131C0001000200022 Natural Killer Cells in Patients with Malignant Non-hodgkin Lymphoma; NK-92MI [NK92MI] 人恶性非霍奇金淋巴瘤患者的自然杀伤细胞；NK-92MI [NK92MI] lymphoblast 淋巴母细胞 suspension growth 悬浮生长 NK-92 is an interleukin-2 dependent NK cell line derived from peripheral blood monocytes of a 50-year-old white male with acute non-Hodgkin's lymphoma. NK-92MI is an IL-2 independent NK cell line derived from NK-92 transfected cells. The parent cells were transformed with human IL-2 cDNA carried by the retroviral MFG-hIL-2 vector used in microsomal gene transformation. Possibly due to the integration of the vector into the genomic DNA, the transformation is stable. This cell is cytotoxic to many malignant cells; chromium release tests show that it can kill K562 and Daudi cells. NK-92 cells have the following characteristics: surface markers of CD2, CD7, CD11a, CD28, CD45 is positive, CD56 bright; surface markers of CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR is negative. The parental IL-2-dependent cell line CRL-2407 (NK92) and another IL-2-independent cell line CRL-2409 (NK-92CI) also derived from the NK-92 cell line are available from the ATCC. Both NK-92MI and NK-92CI variants contain, express and synthesize hIL-2 cDNA. NK-92MI cells synthesize higher levels of IL-2 than NK-92CI, whereas parental cells do not synthesize and express IL-2. Cultures submitted to the ATCC in September 1998 contaminated with Mycoplasma. Mycoplasma was eliminated by its progeny with BM cyclin treatment for 21 days. NK-92是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株白细胞介素-2依赖型NK细胞株。 这株细胞对很多恶性细胞有细胞毒性；铬释放试验显示它能杀死K562和Daudi细胞。 NK-92细胞(经过照射以防止增殖)可以有效地用于血液中白血病的体外免疫清扫而不危及血细胞的功能。 NK-92细胞有以下特征： CD2, CD7, CD11a, CD28, CD45, CD54表面标记阳性，CD56亮；CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34和HLA-DR表面标记阴性。 http://www.cellresource.cn/pdf/20080108100503.pdf http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) horse serum， 12.5% qualified fetal bovine serum，120.005 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NICR480 NK-92MI NK-92MI cell NK-92MI 细胞 NK92MI NK92MI cell NK92MI 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR480 cell NICR480 细胞 http://www.cellresource.cn/fdetail.aspx?id=480 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 URI: http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 URI: http://www.cellresource.cn/pdf/20080108100503.pdf Amelogenin：X；CSF1PO：10，11；D13S317：13；D16S539：10，11；D18S51：13，18；D19S433：11，14；D21S11：28，29；D2S1338：17，23；D3S1358：15，17；D5S818：11；D7S820：8，12；D8S1179：11，15；FGA：20，21；TH01：9.3；TPOX：9；vWA：18； Amelogenin：X；CSF1PO：10，11；D13S317：13；D16S539：10，11；D18S51：13，18；D19S433：11，14；D21S11：28，29；D2S1338：17，23；D3S1358：15，17；D5S818：11；D7S820：8，12；D8S1179：11，15；FGA：20，21；TH01：9.3；TPOX：9；vWA：18； 3111C0001CCC000293 Human Malignant Embryo Rhabdomyoma Cells; RD 人恶性胚胎横纹肌瘤细胞；RD spindle and large polykaryocyte 梭型和大的多核细胞 adherent growth 贴壁生长 The cell line may be the maternal line of TE671. The cells can produce myoglobin and myosin ATPase, and can be used for virus detection. 该细胞可能是TE671的母系。可产生肌红蛋白和肌球蛋白ATPase，可用作病毒检测。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A RD-Species Identification-PUMC RD-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代，3~4天换液1次。 Passage at 1: 2 ~ 1: 4, and change fluid once in 3~4 days. NICR293 RD RD cell RD 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR293 cell NICR293 细胞 http://www.cellresource.cn/fdetail.aspx?id=293 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf RD-Species Identification-PUMC RD-物种鉴定-PUMC Amelogenin:X；CSF1PO:10,11；D13S317:13；D16S539:10,11；D18S51:13,18；D21S11:28,29；D3S1358:15,17；D5S818:11；D7S820:8,12；D8S1179:11,15；FGA:20,21；PentaD:11,13；PentaE:12；TH01:9.3；TPOX:9；vWA:18 Amelogenin:X；CSF1PO:10,11；D13S317:13；D16S539:10,11；D18S51:13,18；D21S11:28,29；D3S1358:15,17；D5S818:11；D7S820:8,12；D8S1179:11,15；FGA:20,21；PentaD:11,13；PentaE:12；TH01:9.3；TPOX:9；vWA:18 3111C0002000000079 Human Malignant Embryo Rhabdomyoma Cells; RD 人恶性胚胎横纹肌瘤细胞；RD spindle and large polykaryocyte 梭型和大的多核细胞 adherent growth 贴壁生长 The cell line may be the maternal line of TE671. The cells can produce myoglobin and myosin ATPase, and can be used for virus detection. 该细胞可能是TE671的母系。可产生肌红蛋白和肌球蛋白ATPase，可用作病毒检测。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P39 P39 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A RD-cell morphology-NIFDC RD-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:2~1:4传代，3~4天换液1次。 Passage at 1: 2 ~ 1: 4, and change fluid once in 3~4 days. NICR451 RD RD cell RD 细胞 NICR451 cell NICR451 细胞 http://www.cellresource.cn/fdetail.aspx?id=451 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf RD-cell morphology-NIFDC RD-细胞形态-NIFDC Amelogenin:X；CSF1PO:10,11；D13S317:13；D16S539:10,11；D18S51:13,18；D19S433:11,14；D21S11:28,29；D2S1338:17,23；D3S1358:15,17；D5S818:11；D7S820:8,12；D8S1179:11,15；FGA:20,21；TH01:9.3；TPOX:9；vWA:18 Amelogenin:X；CSF1PO:10,11；D13S317:13；D16S539:10,11；D18S51:13,18；D19S433:11,14；D21S11:28,29；D2S1338:17,23；D3S1358:15,17；D5S818:11；D7S820:8,12；D8S1179:11,15；FGA:20,21；TH01:9.3；TPOX:9；vWA:18 3131C0001000700045 Human Malignant Embryo Rhabdomyoma Cells; RD 人恶性胚胎横纹肌瘤细胞；RD spindle cells and large multinucleated cells 纺锤形细胞和大的多核细胞 adherent growth 贴壁生长 It has been shown that this cell is at least its parent if it is different from TE 671 (ATCC HTB-139). 目前显示，这株细胞如果跟TE 671 (ATCC HTB-139)不一样，至少也是它的亲本。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A LDH检测报告RD-Isoenzyme-SHCRC LDH检测报告RD-同工酶-SHCRC LDH检测报告RD=3-Isoenzyme-SHCRC LDH检测报告RD=3-同工酶-SHCRC RD-cell morphology-SHCRC RD-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR615 RD RD cell RD 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR615 cell NICR615 细胞 http://www.cellresource.cn/fdetail.aspx?id=615 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf LDH检测报告RD-Isoenzyme-SHCRC LDH检测报告RD-同工酶-SHCRC LDH检测报告RD=3-Isoenzyme-SHCRC LDH检测报告RD=3-同工酶-SHCRC RD-cell morphology-SHCRC RD-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000070 Human Diploid Cells; HEL 人二倍体细胞；HEL fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 HEL is a human diploid cell line with a typical fibroblast morphology with chromosome 2n = 46. It can be used as host for many kinds of viruses, antiaging drugs drug test, etc. HEL是一株人二倍体细胞系，具有典型的成纤维细胞形态，染色体2n＝46。可用于作多种病毒的宿主，抗衰老药物试验等。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C19 C19 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HEL HEL cell HEL 细胞 NICR838 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR838 cell NICR838 细胞 http://www.cellresource.cn/fdetail.aspx?id=838 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin:X；CSF1PO:11；D13S317:9；D16S539:12；D18S51:13；D19S433:14；D21S11:31；D2S1338:17,24；D3S1358:17；D5S818:12；D7S820:11,12；D8S1179:12；FGA:22,24；TH01:6；TPOX:8；vWA:18 Amelogenin:X；CSF1PO:11；D13S317:9；D16S539:12；D18S51:13；D19S433:14；D21S11:31；D2S1338:17,24；D3S1358:17；D5S818:12；D7S820:11,12；D8S1179:12；FGA:22,24；TH01:6；TPOX:8；vWA:18 3131C0001000700153 Human Non-small Cell lung cancer Cells; HCC827 人非小细胞肺癌细胞；HCC827 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell was established in March 1987. This lung adenocarcinoma has an acquired mutation (E746-A750 deletion) in the EGFR tyrosine kinase region. The patient smoked a pack of cigarettes per month between the ages of 25 and 26. The patient was no smoking for 12 years before diagnosis. 这株细胞建于1994年三月。这株肺腺癌在EGFR酪氨酸激酶区域有一个获得性突变(E746-A750缺失)。 患者在25岁到26岁时每个月抽1包烟。 在诊断前12年不再抽烟。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(26+5) P(26+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A HCC827-cell morphology-SHCRC HCC827-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 5 minutes. Passage at 1: 2. The cells can be overgrowth within 4-5 days. 消化5分钟。1:2。4-5天长满。 HCC827 HCC827 cell HCC827 细胞 NICR719 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR719 cell NICR719 细胞 http://www.cellresource.cn/fdetail.aspx?id=719 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf HCC827-cell morphology-SHCRC HCC827-细胞形态-SHCRC Amelogenin:X；CSF1PO:11；D13S317:9；D16S539:12；D18S51:13；D19S433:14；D21S11:31；D2S1338:17,24；D3S1358:17；D5S818:12；D7S820:11,12；D8S1179:12；FGA:22,24；TH01:6；TPOX:8；vWA:18 Amelogenin:X；CSF1PO:11；D13S317:9；D16S539:12；D18S51:13；D19S433:14；D21S11:31；D2S1338:17,24；D3S1358:17；D5S818:12；D7S820:11,12；D8S1179:12；FGA:22,24；TH01:6；TPOX:8；vWA:18 3111C0001CCC000478 Human Non-small Cell lung cancer Cells; HCC827 人非小细胞肺癌细胞；HCC827 epithelium-like; polygonal shape 上皮样；多角形 adherent growth 贴壁生长 This cell was established in March 1987. This lung adenocarcinoma has an acquired mutation (E746-A750 deletion) in the EGFR tyrosine kinase region. The patient smoked a pack of cigarettes per month between the ages of 25 and 26. The patient was no smoking for 12 years before diagnosis. 这株细胞建于1994年三月。这株肺腺癌在EGFR酪氨酸激酶区域有一个获得性突变(E746-A750缺失)。 患者在25岁到26岁时每个月抽1包烟。 在诊断前12年不再抽烟。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代；4-5天传代一次。 Passage at 1: 2; and passage once in 4-5 days. HCC827 HCC827 cell HCC827 细胞 NICR2151 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2151 cell NICR2151 细胞 http://www.cellresource.cn/fdetail.aspx?id=2151 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:12；D13S317:12；D16S539:12,13；D18S51:16；D19S433:14；D21S11:32.2；D2S1338:23,24；D3S1358:17；D5S818:11；D7S820:10；D8S1179:10,13；FGA:20；TH01:6,9.3；TPOX:8；vWA:16,17,18 Amelogenin:X；CSF1PO:12；D13S317:12；D16S539:12,13；D18S51:16；D19S433:14；D21S11:32.2；D2S1338:23,24；D3S1358:17；D5S818:11；D7S820:10；D8S1179:10,13；FGA:20；TH01:6,9.3；TPOX:8；vWA:16,17,18 3131C0001000700160 Human Non-small Cell lung cancer Cells; NCI-H1299 人非小细胞肺癌细胞；NCI-H1299 epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell line is derived from a lymph node metastasis. The patient received initial radiation therapy. The cells are partial uniformly deletion of p53 protein and lack of p53 protein expression. Cells can synthesize 0.1 pmol/mg NMB protein without synthesizing gastrin-releasing peptide (GRP). 这株细胞来源于一个淋巴结转移。 患者接受了初期放疗。 细胞均一性的部分缺失p53蛋白，并缺少p53蛋白表达。 细胞可以合成0.1pmol/毫克蛋白的NMB蛋白，而不合成促胃液释放肽(GRP)。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(47+5) P(47+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A NCI-H1299-cell morphology-SHCRC NCI-H1299-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NCI-H1299 NCI-H1299 cell NCI-H1299 细胞 NICR726 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR726 cell NICR726 细胞 http://www.cellresource.cn/fdetail.aspx?id=726 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H1299-cell morphology-SHCRC NCI-H1299-细胞形态-SHCRC Amelogenin:X；CSF1PO:12；D13S317:12；D16S539:12,13；D18S51:16；D19S433:14；D21S11:32.2；D2S1338:23,24；D3S1358:17；D5S818:11；D7S820:10；D8S1179:10,13；FGA:20；TH01:6,9.3；TPOX:8；vWA:16,17,18 Amelogenin:X；CSF1PO:12；D13S317:12；D16S539:12,13；D18S51:16；D19S433:14；D21S11:32.2；D2S1338:23,24；D3S1358:17；D5S818:11；D7S820:10；D8S1179:10,13；FGA:20；TH01:6,9.3；TPOX:8；vWA:16,17,18 3111C0001CCC000469 Human Non-small Cell lung cancer Cells; NCI-H1299 人非小细胞肺癌细胞；NCI-H1299 epithelium-like; polygonal shape 上皮样；多角形 adherent growth 贴壁生长 This cell line is derived from a lymph node metastasis. The patient received initial radiation therapy. The cells are partial uniformly deletion of p53 protein and lack of p53 protein expression. Cells can synthesize 0.1 pmol/mg NMB protein without synthesizing gastrin-releasing peptide (GRP). 这株细胞来源于一个淋巴结转移。 患者接受了初期放疗。 细胞均一性的部分缺失p53蛋白，并缺少p53蛋白表达。 细胞可以合成0.1pmol/毫克蛋白的NMB蛋白，而不合成促胃液释放肽(GRP)。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代；3天传代一次。 Passage at 1: 2; and passage once within 3 days. NCI-H1299 NCI-H1299 cell NCI-H1299 细胞 NICR2142 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2142 cell NICR2142 细胞 http://www.cellresource.cn/fdetail.aspx?id=2142 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3142C0001000000947 Human Non-small Cell lung cancer Cells; NCI-H1299 人非小细胞肺癌细胞；NCI-H1299 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells, derived from a 43-year-old white man, were isolated from lymph nodes that metastasized from lung cancer before radiation therapy. The cells can be used as a transfection host. 该细胞源于一位43岁的白种男人，分离自接受放疗前由肺癌转移到的淋巴结中。可用作转染宿主。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A H1299-Myoplasma Detection-CCTCC H1299-cell morphology-CCTCC H1299-支原体检测-CCTCC H1299-细胞形态-CCTCC Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NCI-H1299 NCI-H1299 cell NCI-H1299 细胞 NICR2184 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2184 cell NICR2184 细胞 http://www.cellresource.cn/fdetail.aspx?id=2184 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf H1299-Myoplasma Detection-CCTCC H1299-cell morphology-CCTCC H1299-支原体检测-CCTCC H1299-细胞形态-CCTCC 3111C0001CCC000255 Human Non-small Cell lung cancer Cells; NCI-H23 人非小细胞肺癌细胞；NCI-H23 epithelioid 上皮样 adherent growth 贴壁生长 This cell is derived from the tumor tissue of a 51-year-old black patient with non-small cell lung cancer before treatment, expressing C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras RNAs; this cell carries the K-ras 12 mutation; mutation at codon 246 of p53 gene is ATC → ATG; this cell express Heterologous mRNA of PDGF A and B chains, TGF α, TGF β and EGFR; detection for keratin 5, 8 and 18 is positive. the cells are vimentin-positive, neurofilament protein-positive and L-dopa dehydrogenase-negeative. it is reported that the efficiency of cloning of the cells in soft agar is 9.7%. 该细胞源于一位51岁患有非小细胞肺癌黑人男性患者的治疗前的肿瘤组织，表达C-myc、L-myc、v-src、v-abl、v-erb B、c-raf 1、Ha-ras、Ki-ras、N-ras RNAs；该细胞携带K-ras 12突变；p53基因246位密码子突变ATC→ATG；表达PDGF A和B链的异源mRNA；表达TGFα、TGFβ和EGFR；角蛋白 5、8和18阳性，波形蛋白阳性，神经丝蛋白阴性，左旋多巴脱氢酶阴性；据报道，在软琼脂中该细胞形成克隆的效率为9.7%。 RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mMHepes＋1mM sodium pyruvate RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mMHepes＋1mM sodium pyruvate http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A NCI-H23-Species Identification-PUMC NCI-H23-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；3~4天1次。 Passage at 1: 3; and passage once in 3 to 4 days. NCI-H23 NCI-H23 cell NCI-H23 细胞 NICR255 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR255 cell NICR255 细胞 http://www.cellresource.cn/fdetail.aspx?id=255 RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mMHepes＋1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mMHepes＋1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H23-Species Identification-PUMC NCI-H23-物种鉴定-PUMC Amelogenin:X,Y；CSF1PO:11，12；D13S317:8，12；D16S539:12，13；D18S51:14；D19S433:13，14；D21S11:28，30；D2S1338:17，23；D3S1358:14，18；D5S818:10,12；D7S820:10，11；D8S1179:13，14；FGA:20,21；TH01:6；TPOX:8，9；vWA:17 Amelogenin:X,Y；CSF1PO:11，12；D13S317:8，12；D16S539:12，13；D18S51:14；D19S433:13，14；D21S11:28，30；D2S1338:17，23；D3S1358:14，18；D5S818:10,12；D7S820:10，11；D8S1179:13，14；FGA:20,21；TH01:6；TPOX:8，9；vWA:17 3131C0001000700151 Human Non-small Cell lung cancer Cells; NCI-H358 人非小细胞肺癌细胞；NCI-H358 epithelial-like 上皮细胞样 adherent growth 贴壁生长 1981年从一位开始化疗之前的患者的肿瘤组织中分离建株。 超微结构研究表明细胞质中有Clara细胞的特征结构 细胞表达主要的肺表面结合蛋白SP-A的蛋白和RNA。 不表达SP-B和SP-C。 他们在软琼脂中的克隆形成效率为0.83%。 In 1981, the cell line was isolated from tumor tissue of a patient before chemotherapy. Ultrastructural studies showed that the cytoplasm was of Clara cells feature structure expressing the major protein and RNA of pulmonary surfactant associate protein A, SP-A on lung surface. SP-B and SP-C were not expressed. The clone formation efficiency in soft agar was 0.83%. RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(121+5) P(121+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A NCI-H358-cell morphology-SHCRC NCI-H358-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NCI-H358 NCI-H358 cell NCI-H358 细胞 NICR717 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR717 cell NICR717 细胞 http://www.cellresource.cn/fdetail.aspx?id=717 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H358-cell morphology-SHCRC NCI-H358-细胞形态-SHCRC Amelogenin:X,Y；CSF1PO:11，12；D13S317:8，12；D16S539:12，13；D18S51:14；D19S433:13，14；D21S11:28，30；D2S1338:17，23；D3S1358:14，18；D5S818:10,12；D7S820:10，11；D8S1179:13，14；FGA:20,21；TH01:6；TPOX:8，9；vWA:17 Amelogenin:X,Y；CSF1PO:11，12；D13S317:8，12；D16S539:12，13；D18S51:14；D19S433:13，14；D21S11:28，30；D2S1338:17，23；D3S1358:14，18；D5S818:10,12；D7S820:10，11；D8S1179:13，14；FGA:20,21；TH01:6；TPOX:8，9；vWA:17 3111C0001CCC000470 Human Non-small Cell lung cancer Cells; NCI-H358 人非小细胞肺癌细胞；NCI-H358 epithelium-like; polygonal shape 上皮样；多角形 adherent growth 贴壁生长 1981年从一位开始化疗之前的患者的肿瘤组织中分离建株。 超微结构研究表明细胞质中有Clara细胞的特征结构 细胞表达主要的肺表面结合蛋白SP-A的蛋白和RNA。 不表达SP-B和SP-C。 他们在软琼脂中的克隆形成效率为0.83%。 In 1981, the cell line was isolated from tumor tissue of a patient before chemotherapy. Ultrastructural studies showed that the cytoplasm was of Clara cells feature structure expressing the major protein and RNA of pulmonary surfactant associate protein A, SP-A on lung surface. SP-B and SP-C were not expressed. The clone formation efficiency in soft agar was 0.83%. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代；3天传代一次。 Passage at 1: 2; and passage once within 3 days. NCI-H358 NCI-H358 cell NCI-H358 细胞 NICR2143 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2143 cell NICR2143 细胞 http://www.cellresource.cn/fdetail.aspx?id=2143 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：12；D18S51：12，13；D19S433：16；D21S11：29，30；D2S1338：16，17；D3S1358：15；D5S818：12；D7S820：11，12；D8S1179：13，15；FGA：21，22，25；TH01：8，9.3；TPOX：8，10；vWA：14，17； Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：12；D18S51：12，13；D19S433：16；D21S11：29，30；D2S1338：16，17；D3S1358：15；D5S818：12；D7S820：11，12；D8S1179：13，15；FGA：21，22，25；TH01：8，9.3；TPOX：8，10；vWA：14，17； 3111C0001CCC000356 Human Non-small Cell lung cancer Cells; NCI-H524 人非小细胞肺癌细胞；NCI-H524 round 圆形 suspension growth 悬浮生长 This cell line was established in 1982 and originated from the metastatic lymph nodes of male patients with non-small cell lung cancer. 该细胞1982年建系，源自非小细胞肺癌男性患者的转移淋巴结。 RPMI 1640 (w/o Hepes) 10% FBS RPMI 1640 (w/o Hepes) 10% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P21 P21 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com NCI-H524 NCI-H524 cell NCI-H524 细胞 NICR1545 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1545 cell NICR1545 细胞 http://www.cellresource.cn/fdetail.aspx?id=1545 RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3111C0001CCC000258 Human Non-small Cell lung cancer Cells; NCI-H838 人非小细胞肺癌细胞；NCI-H838 epithelioid 上皮样 adherent growth 贴壁生长 The cell was established in 1984 and originated from a 59-year-old white male smoker with non-small cell lung cancer and isolated from a lymph node metastasis of the patient. 该细胞于1984年建系，源于一位59岁患有非小细胞肺癌的白人男性吸烟者，从患者淋巴结转移灶分离而来。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 38~53 38~53 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；2~3天换液1次。 Passage at 1: 3 ~ 1: 6; and change fluid once in 2~3 days. NCI-H838 NCI-H838 cell NCI-H838 细胞 NICR258 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR258 cell NICR258 细胞 http://www.cellresource.cn/fdetail.aspx?id=258 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：12，13；D18S51：13，15；D19S433：11，13；D21S11：32；D2S1338：21，22；D3S1358：17，18；D5S818：10，13；D7S820：12；D8S1179：14，16；FGA：22，23；TH01：7，9；TPOX：6，12；vWA：15； Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：12，13；D18S51：13，15；D19S433：11，13；D21S11：32；D2S1338：21，22；D3S1358：17，18；D5S818：10，13；D7S820：12；D8S1179：14，16；FGA：22，23；TH01：7，9；TPOX：6，12；vWA：15； 3111C0001CCC000113 Non-small Cell Human Lung Cancer Cells; NCI-H157 人非小细胞肺腺癌细胞；NCI-H157 polygonal 多角 adherent growth 贴壁生长 RPMI 1640 (w/o Hepes) 10%FBS；10mM Hepes+1mM sodium pyruvate RPMI 1640 (w/o Hepes) 10%FBS；10mM Hepes+1mM丙铜酸钠 RPMI 1640 (w/o Hepes) 10%FBS；10mM Hepes+1mM丙铜酸钠 http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P86 P86 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 49~54 49~54 A类 Class A NCI-H157-Cell Morphology-PUMC NCI-H157-Species Identification-PUMC NCI-H157-cell morphology-PUMC NCI-H157-物种鉴定-PUMC NCI-H157-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；5~7天1次。 Passage at 1: 3; and passage once in 5 to 7 days. NCI-H157 NCI-H157 cell NCI-H157 细胞 NICR113 PMID:8389448 PMID:8389448 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR113 cell NICR113 细胞 http://www.cellresource.cn/fdetail.aspx?id=113 RPMI 1640 (w/o Hepes) 10%FBS；10mM Hepes+1mM丙铜酸钠 URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；10mM Hepes+1mM丙铜酸钠 URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H157-Cell Morphology-PUMC NCI-H157-Species Identification-PUMC NCI-H157-cell morphology-PUMC NCI-H157-物种鉴定-PUMC NCI-H157-细胞形态-PUMC NCI-H157-细胞形态-PUMC Amelogenin：X；CSF1PO：12；D13S317：10，13；D16S539：9，12；D18S51：13；D19S433：15，15.2；D21S11：28；D2S1338：17；D3S1358：14，15；D5S818：11，12；D7S820：8；D8S1179：13；FGA：21，24；TH01：7；TPOX：8，11；vWA：18； Amelogenin：X；CSF1PO：12；D13S317：10，13；D16S539：9，12；D18S51：13；D19S433：15，15.2；D21S11：28；D2S1338：17；D3S1358：14，15；D5S818：11，12；D7S820：8；D8S1179：13；FGA：21，24；TH01：7；TPOX：8，11；vWA：18； 3111C0001CCC000252 Non-small Cell Human Lung Cancer Cells; NCI-H1975 人非小细胞肺腺癌细胞；NCI-H1975 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from the non-small cell lung adenocarcinoma tissue of a female (without history of smoking) in July 1988. 该细胞是1988年7月从一名女性（无抽烟史）非小细胞肺腺癌组织中分离得到的。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A NCI-H1975-Species Identification-PUMC NCI-H1975-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；每周换液2~3次。 Passage at 1: 3 ~ 1: 6; and change fluid for 2~3 times a week. NCI-H1975 NCI-H1975 cell NCI-H1975 细胞 NICR252 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR252 cell NICR252 细胞 http://www.cellresource.cn/fdetail.aspx?id=252 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H1975-Species Identification-PUMC NCI-H1975-物种鉴定-PUMC Amelogenin：X；CSF1PO：12；D13S317：11；D16S539：9，11；D18S51：13,15,17；D19S433：13,14；D21S11：30,32.2；D2S1338：22,24；D3S1358：15,17；D5S818：11；D7S820：8,10；D8S1179：10,12；FGA：20；TH01：7,9；TPOX：11；vWA：17； Amelogenin：X；CSF1PO：12；D13S317：11；D16S539：9，11；D18S51：13,15,17；D19S433：13,14；D21S11：30,32.2；D2S1338：22,24；D3S1358：15,17；D5S818：11；D7S820：8,10；D8S1179：10,12；FGA：20；TH01：7,9；TPOX：11；vWA：17； 3111C0001CCC000253 Non-small Cell Human Lung Cancer Cells; NCI-H2087 人非小细胞肺腺癌细胞；NCI-H2087 epithelioid 上皮样 suspension growth, a few cells in loosely adherent 悬浮生长，有少数细胞疏松贴壁 The cells were isolated from the lymph node metastasisa of 69-year-old white male (60-year-old) with non-small-cell lung adenocarcinoma in November 1988. 该细胞是1988年11月从一名69岁白人男性（60年烟龄）非小细胞性肺腺癌淋巴结转移组织中分离得到的。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 0.02mg/ml insulin＋0.01mg/ml transferrin + 25nM sodium arsenite +50nMhydrocortisone＋1ng/ml EGF＋0.01mM ethylamide +0.01mM phosphoethanolamine +100pM trimethylamine +0.5%(w/v bovine serum白蛋白＋10mM HEPES＋0.5mM sodium pyruvate＋2mM L-glutamine DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 0.02mg/ml胰岛素＋0.01mg/ml转铁蛋白＋25nM亚砷酸钠＋50nM氢化可的松＋1ng/ml EGF＋0.01mM乙酰胺＋0.01mM磷酸乙醇胺＋100pM三碘甲腺原氨酸＋0.5%(w/v)牛血清白蛋白＋10mM HEPES＋0.5mM sodium pyruvate＋2mM L-谷氨酰胺。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 0.02mg/ml胰岛素＋0.01mg/ml转铁蛋白＋25nM亚砷酸钠＋50nM氢化可的松＋1ng/ml EGF＋0.01mM乙酰胺＋0.01mM磷酸乙醇胺＋100pM三碘甲腺原氨酸＋0.5%(w/v)牛血清白蛋白＋10mM HEPES＋0.5mM sodium pyruvate＋2mM L-谷氨酰胺。 http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；每周换液2次。 Passage at 1: 2 ~ 1: 4; and change fluid for 2 times a week. NCI-H2087 NCI-H2087 cell NCI-H2087 细胞 NICR253 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR253 cell NICR253 细胞 http://www.cellresource.cn/fdetail.aspx?id=253 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 0.02mg/ml胰岛素＋0.01mg/ml转铁蛋白＋25nM亚砷酸钠＋50nM氢化可的松＋1ng/ml EGF＋0.01mM乙酰胺＋0.01mM磷酸乙醇胺＋100pM三碘甲腺原氨酸＋0.5%(w/v)牛血清白蛋白＋10mM HEPES＋0.5mM sodium pyruvate＋2mM L-谷氨酰胺。 URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 0.02mg/ml胰岛素＋0.01mg/ml转铁蛋白＋25nM亚砷酸钠＋50nM氢化可的松＋1ng/ml EGF＋0.01mM乙酰胺＋0.01mM磷酸乙醇胺＋100pM三碘甲腺原氨酸＋0.5%(w/v)牛血清白蛋白＋10mM HEPES＋0.5mM sodium pyruvate＋2mM L-谷氨酰胺。 URI: http://www.cellresource.cn/pdf/20080108100542.pdf 3142C0001000000747 Human Lung Cancer Cells; 225 人肺癌细胞；225 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell is patented and its characteristics have not been disclosed. 该细胞属专利保藏，其特征特性尚未公开。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Undefined 未定 Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3~1：8传代；每周2~4次。 Passage at 1: 3 ~ 1: 8; and passage for 2 to 4 times a week. 225 225 cell 225 细胞 NICR1848 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1848 cell NICR1848 细胞 http://www.cellresource.cn/fdetail.aspx?id=1848 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf 3111C0001CCC000213 Human Lung Cancer Cells; A-427 [A427] 人肺癌细胞；A-427 [A427] epithelioid 上皮样 adherent growth 贴壁生长 The cell line was established by Giard DJ and was derived from a 52-year-old white male patient with lung cancer. 该细胞系由Giard DJ建立，源自一位52岁的肺癌男性白人患者。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1% NEAA,1mM sodium pyruvate MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1% NEAA,1mM sodium pyruvate http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:6传代，6~8天1次；每周换液2次。 Passage at 1: 2 ~ 1: 6, and passage once in 6 to 8 days; and change fluid for 2 times a week. A-427 A-427 cell A-427 细胞 A427 A427 cell A427 细胞 NICR213 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR213 cell NICR213 细胞 http://www.cellresource.cn/fdetail.aspx?id=213 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1% NEAA,1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1% NEAA,1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000700192 Human Lung Cancer Cells; Calu-1 人肺癌细胞；Calu-1 epithelial cell 上皮细胞 adherent growth 贴壁生长 The ultrastructural features includes numerous microvilli, significant RER (rough endoplasmic reticulum), lysosomes, lipid inclusions, and no virus particles. Ras (H-ras) Oncogene is included. 超微结构特征包括众多微绒毛，显著的RER(粗面内质网)，溶酶体，脂包含体，无病毒颗粒。 含ras (H-ras)癌基因。 McCoy＇s 5A Media (Modified with Tricine) qualified fetal bovine serum，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P(25+5) P(25+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Calu-1-cell morphology-SHCRC Calu-1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 10 minutes. Passage at 1: 2. The cells can be overgrowth in 3-4 days 消化10分钟。1:2。3-4天长满 Calu-1 Calu-1 cell Calu-1 细胞 NICR1700 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1700 cell NICR1700 细胞 http://www.cellresource.cn/fdetail.aspx?id=1700 McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf Calu-1-cell morphology-SHCRC Calu-1-细胞形态-SHCRC Amelogenin:X；CSF1PO:12；D13S317:12；D16S539:12,13；D18S51:16；D21S11:32.2；D3S1358:17；D5S818:11；D7S820:10；D8S1179:10,13；FGA:20；PentaD:13；PentaE:11；TH01:6,9.3；TPOX:8；vWA:16,18 Amelogenin:X；CSF1PO:12；D13S317:12；D16S539:12,13；D18S51:16；D21S11:32.2；D3S1358:17；D5S818:11；D7S820:10；D8S1179:10,13；FGA:20；PentaD:13；PentaE:11；TH01:6,9.3；TPOX:8；vWA:16,18 3111C0002000000050 Human Lung Cancer Cells; H1299 人肺癌细胞；H1299 epithelioid cells 上皮样细胞 adherent growth 贴壁生长 This cell line is derived from a lymph node metastasis. The patient received initial radiation therapy. The cells are partial uniformly deletion of p53 protein and lack of p53 protein expression. Cells can synthesize 0.1 pmol/mg NMB protein without synthesizing gastrin-releasing peptide (GRP). 这株细胞来源于一个淋巴结转移。 患者接受了初期放疗。 细胞均一性的部分缺失p53蛋白，并缺少p53蛋白表达。 细胞可以合成0.1pmol/毫克蛋白的NMB蛋白，而不合成促胃液释放肽(GRP)。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A H1299-cell morphology-NIFDC H1299-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 H1299 H1299 cell H1299 细胞 NICR422 NICR422 cell NICR422 细胞 http://www.cellresource.cn/fdetail.aspx?id=422 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf H1299-cell morphology-NIFDC H1299-细胞形态-NIFDC 3131C0001000700161 Human Lung Cancer Cell Line; MSTO-211H 人肺癌细胞株；MSTO-211H fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 MSTO-211H细胞株是1985年从一位肺二相间皮瘤患者的胸水中建株的。 这个病人接受过多种药物联合前期化疗。 MSTO-211H细胞具有高亲和力的EGF结合位点，并表达神经元特异性烯醇酶(NSE)及人绒毛膜促性腺激素(HCG)的α与β亚基。 未检测到左旋多巴胺脱羧酶(DDC)，邦巴辛与神经tensin。 细胞过表达c-myc原癌基因，并没有观察到基因重排或扩增。 V-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, 和 N-ras的表达呈阳性。 未检测到N-m The MSTO-211H cell line was established in 1985 from the hydrothorax of a patient with lung biphasic mesothelioma. The patient had received a multi-drug combination pre-chemotherapy. MSTO-211H cells have high affinity EGF binding sites and express neuron-specific enolase (NSE) and alpha and beta subunits of human chorionic gonadotropin (HCG). No L-dopa decarboxylase (DDC), bombesin and nerve tensin is detected. The cells overexpress the c-myc proto-oncogene and no gene rearrangement or amplification is observed. The expression of V-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras is positive. N-m is not detected RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(21+5) P(21+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 MSTO-211H MSTO-211H cell MSTO-211H 细胞 NICR1678 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1678 cell NICR1678 细胞 http://www.cellresource.cn/fdetail.aspx?id=1678 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3142C0001000000550 Human Lung Cancer Hybrid Cells; 2F7 人肺癌杂交细胞；2F7 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell is patented and its characteristics have not been disclosed. 该细胞属专利保藏，其特征特性尚未公开。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Undefined 未定 Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代，3-4天传1次 Passage at 1: 3, and passage once in 3-4 days. 2F7 2F7 cell 2F7 细胞 NICR1197 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1197 cell NICR1197 细胞 http://www.cellresource.cn/fdetail.aspx?id=1197 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000200028 Human Lung Fibroblast; HFL1 人肺成纤维细胞；HFL1 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 F12K qualified fetal bovine serum，10% F12K 优质胎牛血清，10% F12K 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf P(10+5) P(10+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 HFL1 HFL1 cell HFL1 细胞 NICR485 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR485 cell NICR485 细胞 http://www.cellresource.cn/fdetail.aspx?id=485 F12K 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf Amelogenin：X，Y；CSF1PO：12；D13S317：10；D16S539：10，12；D18S51：17，18；D19S433：13；D21S11：29；D2S1338：19，20；D3S1358：16，17；D5S818：OL；D7S820：10，12；D8S1179：11，OL；FGA：20；TH01：7；TPOX：8，11；vWA：16，17； Amelogenin：X，Y；CSF1PO：12；D13S317：10；D16S539：10，12；D18S51：17，18；D19S433：13；D21S11：29；D2S1338：19，20；D3S1358：16，17；D5S818：OL；D7S820：10，12；D8S1179：11，OL；FGA：20；TH01：7；TPOX：8，11；vWA：16，17； 3111C0001CCC000353 Human Lung Squamous Carcinoma Cells; NCI-H1703 人肺鳞癌细胞；NCI-H1703 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell line was established in 1987 and was derived from a 54-year-old white male with non-small cell lung cancer who was a smoker. 该细胞1987年建系，源自一位54岁患有非小细胞肺癌的白人男性，该患者为吸烟者。 RPMI 1640 (w/o Hepes) 10% FBS RPMI 1640 (w/o Hepes) 10% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P44 P44 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3～1:6传代，每周换液2～3次 Passage at 1: 3 ~ 1: 6, and change fluid for 2 to 3 times a week NCI-H1703 NCI-H1703 cell NCI-H1703 细胞 NICR1542 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1542 cell NICR1542 细胞 http://www.cellresource.cn/fdetail.aspx?id=1542 RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：12；D13S317：9；D16S539：12，14；D18S51：13，14；D19S433：13.2，14；D21S11：31.2；D2S1338：17，24；D3S1358：19；D5S818：12；D7S820：10；D8S1179：14；FGA：25，26；TH01：8，9.3；TPOX：8，9；vWA：15，19； Amelogenin：X；CSF1PO：12；D13S317：9；D16S539：12，14；D18S51：13，14；D19S433：13.2，14；D21S11：31.2；D2S1338：17，24；D3S1358：19；D5S818：12；D7S820：10；D8S1179：14；FGA：25，26；TH01：8，9.3；TPOX：8，9；vWA：15，19； 3111C0001CCC000354 Human Lung Squamous Carcinoma Cells; NCI-H2170 人肺鳞癌细胞；NCI-H2170 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell line was established in 1989 and originated from a man with squamous cell carcinoma of the lung who did not smoke. 该细胞1989年建系，源自一位患有肺鳞状细胞癌的男性，该患者不吸烟。 RPMI 1640 (w/o Hepes) 10% FBS RPMI 1640 (w/o Hepes) 10% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P24 P24 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3～1:6传代，3～5天换液1次 Passage at 1: 3 ~ 1: 6, and change fluid once in 3 to 5 days NCI-H2170 NCI-H2170 cell NCI-H2170 细胞 NICR1543 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1543 cell NICR1543 细胞 http://www.cellresource.cn/fdetail.aspx?id=1543 RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001001200016 Human Lung Squamous Carcinoma Cells; NCI-H226 [H226] 人肺鳞癌细胞；NCI-H226 [H226] epithelioid 上皮样 adherent growth 贴壁生长 1980年分离建立。 It was isolated and established in 1980. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1：4-1：8，每2~3天传代。 Passage at 1: 4-1: 8, and passage in every 2 to 3 days. H226 H226 cell H226 细胞 NCI-H226 NCI-H226 cell NCI-H226 细胞 NICR1597 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1597 cell NICR1597 细胞 http://www.cellresource.cn/fdetail.aspx?id=1597 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3111C0001CCC000197 Human Lung Squamous Carcinoma Cells; NCI-H520 人肺鳞癌细胞；NCI-H520 epithelioid 上皮样 adherent growth 贴壁生长 1982年Gazdar AF等建系，源于鳞状细胞肺癌组织；该细胞p53 mRNA表达水平较正常肺组织低，但是没有大的结构DNA的异常。角蛋白、波形蛋白阳性，神经丝三联蛋白阴性；该细胞在软琼脂中可形成克隆。 It was established from lung squamous cell carcinoma by Gazdar AF in 1982; the expression level of p53 mRNA was lower than that of normal lung tissue, but there is no big structural DNA anomaly. The cells is keratin-positive, vimentin-positive, NF3(neurofilament triple protein)-negative; the cells can form clones in soft agar. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C6 C6 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 91~95 91~95 A类 Class A NCI-H520-Species Identification-PUMC NCI-H520-cell morphology-PUMC NCI-H520-物种鉴定-PUMC NCI-H520-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；2~3天换液1次。 Passage at 1: 3 ~ 1: 6; and change fluid once in 2~3 days. NCI-H520 NCI-H520 cell NCI-H520 细胞 NICR197 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR197 cell NICR197 细胞 http://www.cellresource.cn/fdetail.aspx?id=197 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H520-Species Identification-PUMC NCI-H520-cell morphology-PUMC NCI-H520-物种鉴定-PUMC NCI-H520-细胞形态-PUMC Amelogenin：X，Y；CSF1PO：12；D13S317：11；D16S539：13；D18S51：17；D19S433：14；D21S11：29，30；D2S1338：19；D3S1358：16；D5S818：11；D7S820：8；D8S1179：13，14；FGA：20，24；TH01：6，9.3；TPOX：8；vWA：14； Amelogenin：X，Y；CSF1PO：12；D13S317：11；D16S539：13；D18S51：17；D19S433：14；D21S11：29，30；D2S1338：19；D3S1358：16；D5S818：11；D7S820：8；D8S1179：13，14；FGA：20，24；TH01：6，9.3；TPOX：8；vWA：14； 3111C0001CCC000262 Human Lung Squamous Carcinoma Cells; SK-MES-1 人肺鳞癌细胞；SK-MES-1 epithelioid 上皮样 adherent growth 贴壁生长 It is derived from a 65-year-old white male with squamous cell carcinoma of the lung, isolated from a metastatic pleural effusion. 源于一位65岁患有肺鳞状细胞癌的白人男性，自转移性胸腔积液分离而来。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 52~58 52~58 A类 Class A SK-MES-1-Species Identification-PUMC SK-MES-1-cell morphology-PUMC SK-MES-1-物种鉴定-PUMC SK-MES-1-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代，4~5天1次，每周换液2~3次。 Passage at 1: 3 ~ 1: 6, 4 to 5 days 1, and change fluid for 2~3 times a week. NICR262 SK-MES-1 SK-MES-1 cell SK-MES-1 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR262 cell NICR262 细胞 http://www.cellresource.cn/fdetail.aspx?id=262 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf SK-MES-1-Species Identification-PUMC SK-MES-1-cell morphology-PUMC SK-MES-1-物种鉴定-PUMC SK-MES-1-细胞形态-PUMC 3131C0001000700110 Human Lung Squamous Carcinoma Cells; SK-MES-1 人肺鳞癌细胞；SK-MES-1 epithelial cell 上皮细胞 adherent growth 贴壁生长 The cell line was isolated and cultured by G. Trempe and L. J. Old (1970) from the pleural fluid of a patient with squamous cell carcinoma of the lung. 该细胞系由G. Trempe和L. J. Old（1970）从一个患肺部鳞癌的病人胸水中分离培养。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(23+5) P(23+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A SK-MES-1-DNA-Fingerprinting-SHCRC SK-MES-1-DNA-指纹-SHCRC SK-MES-1-cell morphology-SHCRC SK-MES-1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 5 minutes. Passage at 1: 2. The cells can be overgrowth within 4-5 days. 消化5分钟。1:2。4-5天长满。 NICR678 SK-MES-1 SK-MES-1 cell SK-MES-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR678 cell NICR678 细胞 http://www.cellresource.cn/fdetail.aspx?id=678 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf SK-MES-1-DNA-Fingerprinting-SHCRC SK-MES-1-DNA-指纹-SHCRC SK-MES-1-cell morphology-SHCRC SK-MES-1-细胞形态-SHCRC 3142C0001000000946 Human Lung Squamous Carcinoma Cells; SK-MES-1 人肺鳞癌细胞；SK-MES-1 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell is derived from a 65-year-old white man, isolated from pleural effusion into which lung cancer had metastasized. 该细胞源于一位65岁的白种男人，分离自由肺癌转移到的胸膜积液中。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A SK-MES-1-Myoplasma Detection-CCTCC SK-MES-1-cell morphology-CCTCC SK-MES-1-支原体检测-CCTCC SK-MES-1-细胞形态-CCTCC Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR2183 SK-MES-1 SK-MES-1 cell SK-MES-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2183 cell NICR2183 细胞 http://www.cellresource.cn/fdetail.aspx?id=2183 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf SK-MES-1-Myoplasma Detection-CCTCC SK-MES-1-cell morphology-CCTCC SK-MES-1-支原体检测-CCTCC SK-MES-1-细胞形态-CCTCC AMEL:X,Y；CSF1PO:10,11；D12S391:22,22；D13S317:13,14；D16S539:9,12；D18S51:16,16；D19S433:15,15；D21S11:29,32.2；D2S1338:25,25；D2S441:14,14；D3S1358:16,16；D5S818:11,12；D6S1043:13,18；D7S820:8,10；D8S1179:14,15；FGA:20,23；Penta D:9,10；Penta E:12,15；TH01:8,9.3；TPOX:8,1 AMEL:X,Y；CSF1PO:10,11；D12S391:22,22；D13S317:13,14；D16S539:9,12；D18S51:16,16；D19S433:15,15；D21S11:29,32.2；D2S1338:25,25；D2S441:14,14；D3S1358:16,16；D5S818:11,12；D6S1043:13,18；D7S820:8,10；D8S1179:14,15；FGA:20,23；Penta D:9,10；Penta E:12,15；TH01:8,9.3；TPOX:8,1 3142C0001000000966 Human Lung Squamous Carcinoma Cells; NCI-H226 人肺鳞状癌细胞；NCI-H226 epithelial-like 上皮细胞样 adherent growth 贴壁生长 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NCI-H226 NCI-H226 cell NCI-H226 细胞 NICR2203 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2203 cell NICR2203 细胞 http://www.cellresource.cn/fdetail.aspx?id=2203 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700144 Human Degenerative Lung Cancer Cells; Calu-6 人肺退行性癌细胞；Calu-6 epithelial cell 上皮细胞 adherent growth 贴壁生长 1976年分离得到。 In 1976, it was isolated. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(32+5) P(32+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Calu-6-DNA-Fingerprinting-SHCRC Calu-6-DNA-指纹-SHCRC Calu-6-cell morphology-SHCRC Calu-6-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 20 minutes. Passage at 1: 2. The cells can be overgrowth in 5-6 days. 消化20分钟。1:2。5-6天长满。 Calu-6 Calu-6 cell Calu-6 细胞 NICR710 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR710 cell NICR710 细胞 http://www.cellresource.cn/fdetail.aspx?id=710 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf Calu-6-DNA-Fingerprinting-SHCRC Calu-6-DNA-指纹-SHCRC Calu-6-cell morphology-SHCRC Calu-6-细胞形态-SHCRC 3111C0002000000055 Human Lung Cells; HLF-a 人肺细胞；HLF-a epithelioid cells 上皮样细胞 adherent growth 贴壁生长 P86 P86 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） B类 Class B National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net HLF-a HLF-a cell HLF-a 细胞 NICR427 NICR427 cell NICR427 细胞 http://www.cellresource.cn/fdetail.aspx?id=427 - - 3111C0001CCC000329 Human Lung Adenocarcinoma Cell Line; Calu-3 人肺腺癌瘤株；Calu-3 solid tumor 实体瘤 growth in vivo 体内生长 The patient of cell origin has been treated with cyclophosphamide, bleomycin and adriamycin. The cultured cell lines were transplanted in nude mice to form solid tumors and cryopreserved, which can be used to establish an transplantation model of human lung adenocarcinoma in vivo. 该细胞来源患者已经用环磷酰胺、博来霉素、阿霉素进行过治疗。利用培养的细胞系，进行裸鼠体内移植，形成实体瘤，冻存，可用于建立人肺腺癌的体内移植模型。 - - P1 P1 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） - - A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Cells were prepared into cell suspension and inoculated into immunodeficient mice. 制备成细胞悬液接种至免疫缺陷小鼠。 Calu-3 Calu-3 cell Calu-3 细胞 NICR329 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR329 cell NICR329 细胞 http://www.cellresource.cn/fdetail.aspx?id=329 Amelogenin：X；CSF1PO：11，12；D13S317：12；D16S539：12，14；D18S51：14，17；D19S433：12，13；D21S11：28，30；D2S1338：19；D3S1358：15，18；D5S818：11；D7S820：10，11；D8S1179：11，15；FGA：25；TH01：6，9.3；TPOX：8；vWA：16，17； Amelogenin：X；CSF1PO：11，12；D13S317：12；D16S539：12，14；D18S51：14，17；D19S433：12，13；D21S11：28，30；D2S1338：19；D3S1358：15，18；D5S818：11；D7S820：10，11；D8S1179：11，15；FGA：25；TH01：6，9.3；TPOX：8；vWA：16，17； 3111C0001CCC000032 Human Lung Adenocarcinoma Cell Line; Calu-3 人肺腺癌细胞；Calu-3 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from the pleural fluid of a 25-year-old white male patient with adenocarcinoma of the lung who had been treated with cyclophosphamide, bleomycin and adriamycin. The cells were inoculated into nude mice for tumorigenesis and were used as transfection hosts. 该细胞是从一名25岁的白人男性肺腺癌患者的胸水中分离建立的；该患者曾使用过环磷酰胺、博来霉素、阿霉素进行治疗。该细胞接种至裸鼠可成瘤；可作转染宿主。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 56~63，XX，there are several chromosomal translocations 56~63，XX，有若干染色体易位 A类 Class A Calu-3-Species Identification-PUMC Calu-3-cell morphology-PUMC Calu-3-物种鉴定-PUMC Calu-3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；每周2~3次。　 Passage at 1: 3 ~ 1: 6; and passage for 2 to 3 times a week. Calu-3 Calu-3 cell Calu-3 细胞 NICR32 PMID: 16819186 PMID: 16819186 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR32 cell NICR32 细胞 http://www.cellresource.cn/fdetail.aspx?id=32 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Calu-3-Species Identification-PUMC Calu-3-cell morphology-PUMC Calu-3-物种鉴定-PUMC Calu-3-细胞形态-PUMC 3131C0001000700067 Human Lung Adenocarcinoma Cell Line; Calu-3 人肺腺癌细胞；Calu-3 epithelial cell 上皮细胞 adherent growth 贴壁生长 The cells were isolated from the pleural fluid of a 25-year-old white male patient with adenocarcinoma of the lung who had been treated with cyclophosphamide, bleomycin and adriamycin. The cells were inoculated into nude mice for tumorigenesis and were used as transfection hosts. 该细胞是从一名25岁的白人男性肺腺癌患者的胸水中分离建立的；该患者曾使用过环磷酰胺、博来霉素、阿霉素进行治疗。该细胞接种至裸鼠可成瘤；可作转染宿主。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 20 minutes. Passage at 1: 2. The cells can be overgrowth in 5-6 days. 消化20分钟。1:2。5-6天长满。 Calu-3 Calu-3 cell Calu-3 细胞 NICR635 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR635 cell NICR635 细胞 http://www.cellresource.cn/fdetail.aspx?id=635 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000700157 Human Lung Adenocarcinoma Cell Line; Calu-3 人肺腺癌细胞；Calu-3 epithelial cell 上皮细胞 adherent growth 贴壁生长 The cells were isolated from the pleural fluid of a 25-year-old white male patient with adenocarcinoma of the lung who had been treated with cyclophosphamide, bleomycin and adriamycin. The cells were inoculated into nude mice for tumorigenesis and were used as transfection hosts. 该细胞是从一名25岁的白人男性肺腺癌患者的胸水中分离建立的；该患者曾使用过环磷酰胺、博来霉素、阿霉素进行治疗。该细胞接种至裸鼠可成瘤；可作转染宿主。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(19+5) P(19+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 20 minutes. Passage at 1: 2. The cells can be overgrowth in 5-6 days. 消化20分钟。1:2。5-6天长满。 Calu-3 Calu-3 cell Calu-3 细胞 NICR723 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR723 cell NICR723 细胞 http://www.cellresource.cn/fdetail.aspx?id=723 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3142C0001000000948 Human Lung Adenocarcinoma Cell Line; Calu-3 人肺腺癌细胞；Calu-3 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells, derived from a 25 -year-old white man, were isolated from pleural effusion that had been transferred from lung cancer before chemotherapy. The cells can be used as a transfection host. 该细胞源于一位25岁的白种男人，分离自接受化疗前由肺癌转移到的胸膜积液中。可用作转染宿主。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Calu-3-Myoplasma Detection-CCTCC Calu-3-cell morphology-CCTCC Calu-3-支原体检测-CCTCC Calu-3-细胞形态-CCTCC Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Calu-3 Calu-3 cell Calu-3 细胞 NICR2185 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2185 cell NICR2185 细胞 http://www.cellresource.cn/fdetail.aspx?id=2185 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Calu-3-Myoplasma Detection-CCTCC Calu-3-cell morphology-CCTCC Calu-3-支原体检测-CCTCC Calu-3-细胞形态-CCTCC 3131C0001000700034 Human Lung Adenocarcinoma Cell Line; Lu-165 人肺腺癌细胞；Lu-165 epithelioid cells 上皮样细胞 adherent growth 贴壁生长 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Lu-165 Lu-165 cell Lu-165 细胞 NICR604 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR604 cell NICR604 细胞 http://www.cellresource.cn/fdetail.aspx?id=604 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin:X；CSF1PO:12；D13S317:10,14；D16S539:11,13；D18S51:12,14；D19S433:13,16；D21S11:29；D2S1338:17,24；D3S1358:15；D5S818:12；D7S820:8；D8S1179:12,14；FGA:18,23；TH01:6,9.3；TPOX:8；vWA:14,17 Amelogenin:X；CSF1PO:12；D13S317:10,14；D16S539:11,13；D18S51:12,14；D19S433:13,16；D21S11:29；D2S1338:17,24；D3S1358:15；D5S818:12；D7S820:8；D8S1179:12,14；FGA:18,23；TH01:6,9.3；TPOX:8；vWA:14,17 3131C0001000700163 Human Lung Adenocarcinoma Cell Line; NCI-H1395 人肺腺癌细胞；NCI-H1395 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line was established in April 1986. The patient was a smoker. The smoking amount is 10 bags a year. ATCC CRL-5957 is a lymphatic-like cell derived from the same patient. 这株细胞是1986年4月建株的。患者吸烟。 每年15包。 ATCC CRL-5957是一株来源于同一患者的类淋巴母细胞。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(23+5) P(23+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 5 minutes. Passage at 1: 2. The cells can be overgrowth within 5-6 days. 消化5分钟。1:2。5-6天长满。 NCI-H1395 NCI-H1395 cell NCI-H1395 细胞 NICR727 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR727 cell NICR727 细胞 http://www.cellresource.cn/fdetail.aspx?id=727 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:12；D13S317:10,14；D16S539:11,13；D18S51:12,14；D19S433:13,16；D21S11:29；D2S1338:17,24；D3S1358:15；D5S818:12；D7S820:8；D8S1179:12,14；FGA:18,23；TH01:6,9.3；TPOX:8；vWA:14,17 Amelogenin:X；CSF1PO:12；D13S317:10,14；D16S539:11,13；D18S51:12,14；D19S433:13,16；D21S11:29；D2S1338:17,24；D3S1358:15；D5S818:12；D7S820:8；D8S1179:12,14；FGA:18,23；TH01:6,9.3；TPOX:8；vWA:14,17 3111C0001CCC000476 Human Lung Adenocarcinoma Cell Line; NCI-H1395 人肺腺癌细胞；NCI-H1395 epithelium-like; polygonal shape 上皮样；多角形 adherent growth 贴壁生长 This cell line was established in April 1986. The patient was a smoker. The smoking amount is 10 bags a year. ATCC CRL-5957 is a lymphatic-like cell derived from the same patient. 这株细胞是1986年4月建株的。患者吸烟。 每年15包。 ATCC CRL-5957是一株来源于同一患者的类淋巴母细胞。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代；5-6天传代一次。 Passage at 1: 2; and passage once in 5-6 days. NCI-H1395 NCI-H1395 cell NCI-H1395 细胞 NICR2149 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2149 cell NICR2149 细胞 http://www.cellresource.cn/fdetail.aspx?id=2149 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:12；D13S317:10；D16S539:9,12；D18S51:13；D19S433:15,15.2；D21S11:28；D2S1338:17；D3S1358:14,15；D5S818:11,12；D7S820:8,11；D8S1179:13,16；FGA:21,24；TH01:7；TPOX:8,11；vWA:18 Amelogenin:X；CSF1PO:12；D13S317:10；D16S539:9,12；D18S51:13；D19S433:15,15.2；D21S11:28；D2S1338:17；D3S1358:14,15；D5S818:11,12；D7S820:8,11；D8S1179:13,16；FGA:21,24；TH01:7；TPOX:8,11；vWA:18 3131C0001000700193 Human Lung Adenocarcinoma Cell Line; NCI-H1975 人肺腺癌细胞；NCI-H1975 epithelioid 上皮样 adherent growth 贴壁生长 The cell line was established in July 1988. The tissue provider is a non-smoker. 这株细胞于1988年七月建株。 组织提供者是一位非吸烟人士。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(23+5) P(23+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 5 minutes. Passage at 1: 2. The cells can be overgrowth within 3-4 days. 消化5分钟。1:2。3-4天长满。 NCI-H1975 NCI-H1975 cell NCI-H1975 细胞 NICR1701 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1701 cell NICR1701 细胞 http://www.cellresource.cn/fdetail.aspx?id=1701 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：10，12；D13S317：12；D16S539：12；D18S51：14；D19S433：14，16，17；D21S11：29；D2S1338：16，19；D3S1358：16；D5S818：13；D7S820：9，11；D8S1179：10，15；FGA：22；TH01：9.3；TPOX：8；vWA：14； Amelogenin：X；CSF1PO：10，12；D13S317：12；D16S539：12；D18S51：14；D19S433：14，16，17；D21S11：29；D2S1338：16，19；D3S1358：16；D5S818：13；D7S820：9，11；D8S1179：10，15；FGA：22；TH01：9.3；TPOX：8；vWA：14； 3111C0001CCC000383 Human Lung Adenocarcinoma Cell Line; NCI-H2009 人肺腺癌细胞；NCI-H2009 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line was established in 1983 and originated from metastatic lymph nodes in a 68-year-old female patient with lung adenocarcinoma. 该细胞1983年建系，源自68岁肺腺癌女性患者的转移淋巴结。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS；0.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30nM Sodium selenite + 10 nM Hydrocortisone + 10 nM beta-estradiol + extra 2mM L-glutamine DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS；0.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30nM Sodium selenite + 10 nM Hydrocortisone + 10 nM beta-estradiol + extra 2mM L-glutamine http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf P25 P25 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com NCI-H2009 NCI-H2009 cell NCI-H2009 细胞 NICR1572 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1572 cell NICR1572 细胞 http://www.cellresource.cn/fdetail.aspx?id=1572 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS；0.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30nM Sodium selenite + 10 nM Hydrocortisone + 10 nM beta-estradiol + extra 2mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS；0.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30nM Sodium selenite + 10 nM Hydrocortisone + 10 nM beta-estradiol + extra 2mM L-glutamine URI: http://www.cellresource.cn/pdf/20080108100542.pdf STR Identification STR鉴定 3142C0001000000334 Human Lung Adenocarcinoma Cell Line; NCI-H441 人肺腺癌细胞；NCI-H441 epithelial-like 上皮细胞样 adherent growth 贴壁生长 NCI-H441 was established in 1982 (A.F. Gazdar, et al.). The cells were isolated from pericardial fluid of a patient with lung adenocarcinoma. The cells can form clones in semi-solid agarose and express alveolar surfactant protein A. The doubling time of the cells is 58 hours in serum-containing medium and 99-138 hours in serum-free medium. NCI-H441建系于1982年（A.F. Gazdar，et al.)。该细胞分离自一名肺腺癌患者的心包液。该细胞能在半固体琼脂糖中形成克隆，并能表达肺泡表面活性蛋白A。该细胞在有血清培养基中倍增时间为58小时，在无血清培养基中倍增时间为99-138小时。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C10 C10 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A NCI-H441-Myoplasma Detection-CCTCC NCI-H441-cell morphology-CCTCC NCI-H441-支原体检测-CCTCC NCI-H441-细胞形态-CCTCC Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NCI-H441 NCI-H441 cell NCI-H441 细胞 NICR1985 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1985 cell NICR1985 细胞 http://www.cellresource.cn/fdetail.aspx?id=1985 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H441-Myoplasma Detection-CCTCC NCI-H441-cell morphology-CCTCC NCI-H441-支原体检测-CCTCC NCI-H441-细胞形态-CCTCC AMEL:X,Y；CSF1PO:11,12；D12S391:19.3,19.3；D13S317:9,9；D16S539:9,13；D18S51:18,19；D19S433:14,14；D21S11:32.2,32.2；D2S1338:17,19；D2S441:14,14；D3S1358:18,18；D5S818:11,12；D6S1043:17,17；D7S820:10,10；D8S1179:8,14；FGA:24,25；Penta D:10,12；Penta E:12,12；TH01:9.3,9.3；T AMEL:X,Y；CSF1PO:11,12；D12S391:19.3,19.3；D13S317:9,9；D16S539:9,13；D18S51:18,19；D19S433:14,14；D21S11:32.2,32.2；D2S1338:17,19；D2S441:14,14；D3S1358:18,18；D5S818:11,12；D6S1043:17,17；D7S820:10,10；D8S1179:8,14；FGA:24,25；Penta D:10,12；Penta E:12,12；TH01:9.3,9.3；T 3142C0001000000951 Human Lung Adenocarcinoma Cell Line; NCI-H441 人肺腺癌细胞；NCI-H441 epithelial-like 上皮细胞样 adherent growth 贴壁生长 In 1982, the cells were taken from the pericardial fluid of a male patient with papillary adenocarcinoma of the lung, and the cell line expressed mRNA and protein of the major surfactant apoprotein (SP-A). The electron microscope showed that the cell was multi-layered, flake-like and had cytoplasm structure similar to that of cilia-free secretory cell particles. The cells may be cloned in soft agar with or without serum. 该细胞于1982年取自一位男性肺乳头状腺癌患者的心包夜，该细胞系表达主要表面活性剂的脱辅基蛋白（SP-A）的mRNA和蛋白质。 电子显微镜显示细胞多层薄片状和细胞质结构类似无纤毛分泌细胞颗粒。细胞可以在含或不含血清软琼脂克隆。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NCI-H441 NCI-H441 cell NCI-H441 细胞 NICR2188 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2188 cell NICR2188 细胞 http://www.cellresource.cn/fdetail.aspx?id=2188 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：12，13；D13S317：11；D16S539：11；D18S51：10.2，18；D19S433：13，14；D21S11：29；D2S1338：22，23；D3S1358：14；D5S818：11，13；D7S820：11；D8S1179：13，14；FGA：23，25；TH01：7，9.3；TPOX：8；vWA：16； Amelogenin：X；CSF1PO：12，13；D13S317：11；D16S539：11；D18S51：10.2，18；D19S433：13，14；D21S11：29；D2S1338：22，23；D3S1358：14；D5S818：11，13；D7S820：11；D8S1179：13，14；FGA：23，25；TH01：7，9.3；TPOX：8；vWA：16； 3111C0001CCC000257 Human Lung Adenosquamous Carcinoma Cells; NCI-H596 人肺腺鳞癌细胞；NCI-H596 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated in 1983 by Gazdar AF and Oie H from a chest wall mass in a 73-year-old white male patient with adenosquamous carcinoma of the lung; the mass was harvested prior to treatment. This cell expressed p53 at a level comparable to normal lung tissue without large structural DNA abnormalities. Detctions for keratin and vimentin were positive, while that for neurofilament triple protein was negative; the cells express various markers of squamous cell differentiation, such as transglutaminase activity, production of involucrin and formation of crosslinked envelope. 该细胞是1983年由Gazdar AF和Oie H从一名患有肺腺鳞癌的73岁白人男性患者的胸壁肿物中分离得到；肿块的获取先于治疗。该细胞表达与正常肺组织水平相当的p53，没有大的结构DNA的异常。角蛋白和波形蛋白阳性，神经丝三联蛋白阴性；表达多种鳞状细胞分化标志，如谷氨酰胺转移酶活性、被膜素的产生和交联包膜的形成。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代；每周换液2~3次。 Passage at 1: 4 ~ 1: 8; and change fluid for 2~3 times a week. NCI-H596 NCI-H596 cell NCI-H596 细胞 NICR257 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR257 cell NICR257 细胞 http://www.cellresource.cn/fdetail.aspx?id=257 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：10；D13S317：11，12；D16S539：9，13；D18S51：16；D19S433：12，13.2；D21S11：28；D2S1338：21，24；D3S1358：16；D5S818：13；D7S820：10；D8S1179：11，14；FGA：22，26；TH01：8；TPOX：8，11；vWA：16； Amelogenin：X；CSF1PO：10；D13S317：11，12；D16S539：9，13；D18S51：16；D19S433：12，13.2；D21S11：28；D2S1338：21，24；D3S1358：16；D5S818：13；D7S820：10；D8S1179：11，14；FGA：22，26；TH01：8；TPOX：8，11；vWA：16； 3111C0001CCC000233 Human Pulmonary Mucoepidermoid Carcinoma Cells; NCI-H292 人肺粘液上皮样癌细胞；NCI-H292 epithelioid 上皮样 adherent growth 贴壁生长 The cell line is derived from the lymph node metastasis of bronchus mucoepithelioid carcinoma; it is isolated from a defined medium and then cultured in a serum-containing medium. Under the culture conditions, the cells retain the characteristics of mucinous epithelium, which can be indentified by the expression of their ultrastructure and the expression of various differentiation markers of squamous epithelium. This cell supports the growth of HBV and is negative for levodopa decarboxylase detection. This cell can be used as a screening model and human subgenomic fragments can be transfected into cells to study the role of HBV and its own genes in viral hepatitis and hepatocarcinogenesis. The cytokeratin, vimentin and mucicarmine stainings are positive , but the neurofilament triple protein detection is negative. 该细胞株源自肺支气管黏液上皮样癌的淋巴结转移灶；先用成份限定的培养基分离得到，然后换成含血清的培养基培养。培养条件下该细胞保留了黏液上皮的特性，可从其超微结构的表现和多种鳞状上皮分化标记的表达而判断。该细胞支持HBV的生长，左旋多巴脱羧酶阴性。该细胞可以作为筛选模型，将人类亚基因组片段转染入细胞来研究HBV及其自身基因在病毒性肝炎和肝癌发生中的作用。该细胞角蛋白、波形蛋白、黏液洋红染色阳线，但神经丝三联蛋白阴性。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P84 P84 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:8传代；2~3天换液1次。 Passage at 1: 3 ~ 1: 8; and change fluid once in 2~3 days. NCI-H292 NCI-H292 cell NCI-H292 细胞 NICR233 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR233 cell NICR233 细胞 http://www.cellresource.cn/fdetail.aspx?id=233 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:10；D13S317:11,12；D16S539:9,13；D18S51:16；D19S433:12,13.2；D21S11:28；D2S1338:21,24；D3S1358:16；D5S818:13；D7S820:10；D8S1179:11,14；FGA:22,26；TH01:8；TPOX:8,11；vWA:16,17 Amelogenin:X；CSF1PO:10；D13S317:11,12；D16S539:9,13；D18S51:16；D19S433:12,13.2；D21S11:28；D2S1338:21,24；D3S1358:16；D5S818:13；D7S820:10；D8S1179:11,14；FGA:22,26；TH01:8；TPOX:8,11；vWA:16,17 3131C0001000700122 Human Pulmonary Mucoepidermoid Carcinoma Cells; NCI-H292 人肺粘液上皮样癌细胞；NCI-H292 epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell line is derived from the lymph node metastasis of lung epithelial cell carcinoma. This cell line was isolated from a chemical synthesis medium and transformed to culture in a serum-containing culture medium. When cultured, the cells retain the characteristics of mucosal epithelial cells on the submicroscopic structure and show multiple markers of squamous differentiation. 此细胞株源自肺粘膜上皮细胞癌的淋巴结转移灶。 这个细胞系用化学合成培养基分离得到，并转换成含血清培养液培养。 此细胞在培养时在亚显微结构上保留了粘膜上皮细胞的特性，并表现出鳞状分化的多种标记。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A NCI-H292-DNA-Fingerprinting-SHCRC NCI-H292-DNA-指纹-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NCI-H292 NCI-H292 cell NCI-H292 细胞 NICR690 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR690 cell NICR690 细胞 http://www.cellresource.cn/fdetail.aspx?id=690 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H292-DNA-Fingerprinting-SHCRC NCI-H292-DNA-指纹-SHCRC Amelogenin：X；CSF1PO：11；D13S317：11；D16S539：11，12；D18S51：10；D19S433：15；D21S11：30；D2S1338：19；D3S1358：18；D5S818：11；D7S820：8，9；D8S1179：12；FGA：20；TH01：9.3；TPOX：11；vWA：18； Amelogenin：X；CSF1PO：11；D13S317：11；D16S539：11，12；D18S51：10；D19S433：15；D21S11：30；D2S1338：19；D3S1358：18；D5S818：11；D7S820：8，9；D8S1179：12；FGA：20；TH01：9.3；TPOX：11；vWA：18； 3111C0001CCC000251 Human Lung Cancer Cells; NCI-H1650 人肺支气管癌细胞；NCI-H1650 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from the pleural effusion of a 27-year-old white male (smoking fro 10 years) with bronchoalveolar carcinoma. 该细胞是从一名27岁白人男性（10年烟龄）支气管肺泡癌患者的胸腔积液中分离得到的。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A NCI-H1650-Species Identification-PUMC NCI-H1650-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:6传代；2~3天换液1次。 Passage at 1: 4 ~ 1: 6; and change fluid once in 2~3 days. NCI-H1650 NCI-H1650 cell NCI-H1650 细胞 NICR251 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR251 cell NICR251 细胞 http://www.cellresource.cn/fdetail.aspx?id=251 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H1650-Species Identification-PUMC NCI-H1650-物种鉴定-PUMC Amelogenin:X；CSF1PO:11；D13S317:11；D16S539:11,12；D18S51:10；D19S433:15；D21S11:30；D2S1338:19；D3S1358:18；D5S818:11；D7S820:8,9；D8S1179:12；FGA:20；TH01:9.3；TPOX:11；vWA:18 Amelogenin:X；CSF1PO:11；D13S317:11；D16S539:11,12；D18S51:10；D19S433:15；D21S11:30；D2S1338:19；D3S1358:18；D5S818:11；D7S820:8,9；D8S1179:12；FGA:20；TH01:9.3；TPOX:11；vWA:18 3131C0001000700152 Human Lung Cancer Cells; NCI-H1650 人肺支气管癌细胞；NCI-H1650 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell was established in May 1987. The patient was a smoker. The smoking amount is 10 bags a year. 这株细胞建于1987年五月。患者吸烟。 每年10包。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(24+5) P(24+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A NCI-H1650-cell morphology-SHCRC NCI-H1650-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NCI-H1650 NCI-H1650 cell NCI-H1650 细胞 NICR718 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR718 cell NICR718 细胞 http://www.cellresource.cn/fdetail.aspx?id=718 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H1650-cell morphology-SHCRC NCI-H1650-细胞形态-SHCRC Amelogenin:X；CSF1PO:8；D13S317:12,14；D16S539:10；D18S51:20；D19S433:12.2,14；D21S11:30,31；D2S1338:21,25；D3S1358:15；D5S818:13；D7S820:8,10；D8S1179:12；FGA:18；TH01:6,7；TPOX:9；vWA:17 Amelogenin:X；CSF1PO:8；D13S317:12,14；D16S539:10；D18S51:20；D19S433:12.2,14；D21S11:30,31；D2S1338:21,25；D3S1358:15；D5S818:13；D7S820:8,10；D8S1179:12；FGA:18；TH01:6,7；TPOX:9；vWA:17 3131C0001000700106 Human Hepatocellular Carcinoma Cells; Hep 3B2.1-7 人肝癌细胞；Hep 3B2.1-7 epithelial cell 上皮细胞 adherent growth 贴壁生长 The cell line is from the tissue of an 8-year-old black male. The number of chromosome patterns is 60, which can cause tumor in nude mice. It is known that this cell line contains factors associated with human disease and needs to be operated under level 2 biosafety protection. 该细胞系来自8岁男性黑人的组织。 其染色体模式数目为60，在裸鼠中能致瘤。 已知该细胞系含有与人类疾病有关的因子，需在2级生物安全防护下操作。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(154+5) P(154+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 Hep 3B2.1-7 Hep 3B2.1-7 cell Hep 3B2.1-7 细胞 NICR674 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR674 cell NICR674 细胞 http://www.cellresource.cn/fdetail.aspx?id=674 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin：X，Y；CSF1PO：10，11；D13S317：9，13；D16S539：12，13；D18S51：13，14；D19S433：15.2；D21S11：29，31；D2S1338：19，20；D3S1358：15，16；D5S818：11，12；D7S820：10；D8S1179：15，16；FGA：22，25；TH01：9；TPOX：8，9；vWA：17； Amelogenin：X，Y；CSF1PO：10，11；D13S317：9，13；D16S539：12，13；D18S51：13，14；D19S433：15.2；D21S11：29，31；D2S1338：19，20；D3S1358：15，16；D5S818：11，12；D7S820：10；D8S1179：15，16；FGA：22，25；TH01：9；TPOX：8，9；vWA：17； 3111C0001CCC000035 Human Hepatocellular Carcinoma Cells; Hep G2 [HepG2] 人肝癌细胞；Hep G2 [HepG2] epithelioid 上皮样 adherent growth 贴壁生长 The cells are derived from liver cancer tissue of a 15-year-old white teenager. The cells express alpha-fetoprotein, albumin, alpha-2-macroglobulin, alpha-1-antitrypsin, transferrin, alpha-1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen, complement C4, C3 activator, fibrinogen, alpha-1-acid glycoprotein, α-2-HS-glycoprotein, β-lipoprotein, retinol-binding protein, insulin receptor and receptor for insulin-like growth factor; the cells are capable of 3-hydroxy-3-formyl-CoA reductase and hepatic triglyceride lipase activity. The presence of the HBV genome in this cell has not yet been demonstrated. 该细胞来源于一名15岁的白人少年的肝癌组织。该细胞表达甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗胰蛋白酶、转铁蛋白、α-1-抗凝乳蛋白酶、结合珠蛋白、铜蓝蛋白、纤溶酶原、补体C4、C3激活物、纤维蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、视黄醇结合蛋白；表达胰岛素受体和胰岛素样生长因子IGFⅡ的受体；该细胞具有3-羟基-3-甲酰辅酶A还原酶和肝甘油三酯脂肪酶的活性。目前尚未证明该细胞中有HBV基因组。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 44~52，XY 44~52，XY A类 Class A Hep G2-Species Identification-PUMC Hep G2-cell morphology-PUMC Hep G2-物种鉴定-PUMC Hep G2-细胞形态-PUMC HepG2-Cell Morphology-PUMC HepG2-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1：6传代；每周2次。 Passage at 1: 4 ~ 1: 6; and passage for 2 times a week. Hep G2 Hep G2 cell Hep G2 细胞 HepG2 HepG2 cell HepG2 细胞 NICR35 PMID: 9169468 PMID: 9169468 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR35 cell NICR35 细胞 http://www.cellresource.cn/fdetail.aspx?id=35 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf Hep G2-Species Identification-PUMC Hep G2-cell morphology-PUMC Hep G2-物种鉴定-PUMC Hep G2-细胞形态-PUMC HepG2-Cell Morphology-PUMC HepG2-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:10,11；D13S317:9,13；D16S539:12；D18S51:13，14；D19S433:15.2；D21S11:29,31；D2S1338:19,20；D3S1358:15,16；D5S818:11,12；D7S820:10；D8S1179:15,16；FGA:22,25；TH01:9；TPOX:8,9；vWA:17 Amelogenin:X,Y；CSF1PO:10,11；D13S317:9,13；D16S539:12；D18S51:13，14；D19S433:15.2；D21S11:29,31；D2S1338:19,20；D3S1358:15,16；D5S818:11,12；D7S820:10；D8S1179:15,16；FGA:22,25；TH01:9；TPOX:8,9；vWA:17 3131C0001000700072 Human Hepatocellular Carcinoma Cells; Hep G2 [HepG2] 人肝癌细胞；Hep G2 [HepG2] epithelial cell 上皮细胞 adherent growth 贴壁生长 The cell line is from the tissue of a 15-year-old white male. The morphology is epithelioid and the number of model chromosomes is 55. No tumor has been found in immunosuppressed mice. 该细胞系来自15岁男性白人的组织。 形态为上皮形，模式染色体数为55，在免疫抑制小鼠中不致瘤。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(76+5) P(76+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 Hep G2 Hep G2 cell Hep G2 细胞 HepG2 HepG2 cell HepG2 细胞 NICR640 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR640 cell NICR640 细胞 http://www.cellresource.cn/fdetail.aspx?id=640 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3142C0001000000024 Human Hepatocellular Carcinoma Cells; Hep G2 [HepG2] 人肝癌细胞；Hep G2 [HepG2] epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from liver cancer tissue of a 15-year-old white teenager. The cells express alpha-fetoprotein, albumin, alpha-2-macroglobulin, alpha-1-antitrypsin, transferrin, alpha-1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen, complement C4, C3 activator, fibrinogen, alpha-1-acid glycoprotein, α-2-HS-glycoprotein, β-lipoprotein, retinol-binding protein, insulin receptor and receptor for insulin-like growth factor; the cells are capable of 3-hydroxy-3-formyl-CoA reductase and hepatic triglyceride lipase activity. The presence of the HBV genome in this cell has not yet been demonstrated. 该细胞来源于一名15岁的白人少年的肝癌组织。该细胞表达甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗胰蛋白酶、转铁蛋白、α-1-抗凝乳蛋白酶、结合珠蛋白、铜蓝蛋白、纤溶酶原、补体C4、C3激活物、纤维蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、视黄醇结合蛋白；表达胰岛素受体和胰岛素样生长因子IGFⅡ的受体；该细胞具有3-羟基-3-甲酰辅酶A还原酶和肝甘油三酯脂肪酶的活性。目前尚未证明该细胞中有HBV基因组。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C76 C76 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Hep G2 Hep G2 cell Hep G2 细胞 HepG2 HepG2 cell HepG2 细胞 NICR792 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR792 cell NICR792 细胞 http://www.cellresource.cn/fdetail.aspx?id=792 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin:X；CSF1PO:8；D13S317:12,14；D16S539:10；D18S51:20；D21S11:30,31；D3S1358:15；D5S818:13；D7S820:8,10；D8S1179:12；FGA:18；PentaD:12,14；PentaE:5,16；TH01:6,7；TPOX:9；vWA:17 Amelogenin:X；CSF1PO:8；D13S317:12,14；D16S539:10；D18S51:20；D21S11:30,31；D3S1358:15；D5S818:13；D7S820:8,10；D8S1179:12；FGA:18；PentaD:12,14；PentaE:5,16；TH01:6,7；TPOX:9；vWA:17 3111C0002000000059 Human Hepatocellular Carcinoma Cells; Hep-3B2.1-7 人肝癌细胞；Hep-3B2.1-7 epithelium-like; polygonal shape; growing in clusters 上皮样；多角形；成堆生长 adherent growth 贴壁生长 Hep-3b cells were established in 1979 and originated from a seven-year-old black boy with liver cancer. The cells produce alpha-fetoprotein, hepatitis B surface antigen (HBsAg), albumin, macroglobulin, alpha-antitrypsin, fibronectin, etc, with extensive research value. Hep-3b细胞建系于1979年，源自一位患有肝癌的7岁黑人男童。该细胞产生甲胎蛋白（alpha-fetoprotein)、乙肝表面抗原（BHsAg) 、白蛋白、巨球蛋白、α- 抗胰蛋白酶（alphal-antitrypsin)、转铁蛋白、补体（C3)、C3活性载体，纤维原等，具有广泛的研究价值。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Hep3B2-1.7-cell morphology-NIFDC Hep3B2-1.7-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:4～1:6传代，每周换液2次 Passage at 1: 4 ~ 1: 6, and change fluid for 2 times a week Hep-3B2.1-7 Hep-3B2.1-7 cell Hep-3B2.1-7 细胞 NICR431 NICR431 cell NICR431 细胞 http://www.cellresource.cn/fdetail.aspx?id=431 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Hep3B2-1.7-cell morphology-NIFDC Hep3B2-1.7-细胞形态-NIFDC Amelogenin：XY；CSF1PO:10,11；D13S317:9,13；D16S539:,13；D18S51:13,14；D19S433:15.2；D21S11:29,31；D2S1338:19,20；D3S1358:15,16；D5S818:11,12；D7S820:10；D8S1179:15,16；FGA:22,25；TH01:9；THOX:8,9；vWA:17； Amelogenin：XY；CSF1PO:10,11；D13S317:9,13；D16S539:,13；D18S51:13,14；D19S433:15.2；D21S11:29,31；D2S1338:19,20；D3S1358:15,16；D5S818:11,12；D7S820:10；D8S1179:15,16；FGA:22,25；TH01:9；THOX:8,9；vWA:17； 3153C0001000000220 Human Hepatocellular Carcinoma Cells; HepG2 人肝癌细胞；HepG2 epithelioid cells 上皮样细胞 adherent growth 贴壁生长 This cell line is derived from the hepatocellular carcinoma tissuea of a 15-year-old white male. The number of model chromosomes is 55, and there is a rearranged chromosome 1. This cell has no tumorigenicity to nude mice. The genome of HBV was not detected. 该细胞系来源于一15岁男性白人的肝细胞癌组织。模式染色体数为55条，有一条重排的1号染色体。此细胞对裸鼠没有致瘤性。未检测到HBV的基因组。 10%FBS 10%FBS PN3 PN3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） None 无 50-64 50-64 A类 Class A HepG2-cell morphology-KCB HepG2-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1:3传代；3-4天一次 Passage at 1: 3; and passage once in 3-4 days HepG2 HepG2 cell HepG2 细胞 NICR2171 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2171 cell NICR2171 细胞 http://www.cellresource.cn/fdetail.aspx?id=2171 HepG2-cell morphology-KCB HepG2-细胞形态-KCB Amelogenin:X；CSF1PO:11；D13S317:10,11；D16S539:10；D18S51:15；D19S433:13,14；D21S11:30；D2S1338:19；D3S1358:15；D5S818:12；D7S820:11；D8S1179:14,15；FGA:22；TH01:7；TPOX:8,11；vWA:16,18 Amelogenin:X；CSF1PO:11；D13S317:10,11；D16S539:10；D18S51:15；D19S433:13,14；D21S11:30；D2S1338:19；D3S1358:15；D5S818:12；D7S820:11；D8S1179:14,15；FGA:22；TH01:7；TPOX:8,11；vWA:16,18 3131C0001000700182 Human Hepatocellular Carcinoma Cells; HuH-7 人肝癌细胞；HuH-7 epithelial-like 上皮细胞样 adherent growth 贴壁生长 It is said that the cells can produce alpha fetoprotein, pancreatin alpha antibody, plasma ceruloplasmin, fibrinogen, fibronectin and so on. 据说产甲胎蛋白，胰酶α抗体，血浆铜蓝蛋白，纤维蛋白原，纤维粘连蛋白等。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Huh-7-cell morphology-SHCRC Huh-7-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 HuH-7 HuH-7 cell HuH-7 细胞 NICR1692 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1692 cell NICR1692 细胞 http://www.cellresource.cn/fdetail.aspx?id=1692 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf Huh-7-cell morphology-SHCRC Huh-7-细胞形态-SHCRC Amelogenin：X；CSF1PO:11；D13S317:10；D16S539:10；D18S51:15；D19S433:13,14；D21S11:30；D2S1338:19；D3S1358:15；D5S818:12；D7S820:11；D8S1179:14,15；FGA:22,23；TH01:7；THOX:8,11；vWA:16,18； Amelogenin：X；CSF1PO:11；D13S317:10；D16S539:10；D18S51:15；D19S433:13,14；D21S11:30；D2S1338:19；D3S1358:15；D5S818:12；D7S820:11；D8S1179:14,15；FGA:22,23；TH01:7；THOX:8,11；vWA:16,18； 3153C0001000000221 Human Hepatocellular Carcinoma Cells; Huh-7 人肝癌细胞；Huh-7 epithelioid cells 上皮样细胞 adherent growth 贴壁生长 This cell produces alpha fetoprotein, Pancreatin alpha antibody, plasma ceruloplasmin, fibrinogen, fibronectin and so on. 此细胞产甲胎蛋白，胰酶α抗体，血浆铜蓝蛋白，纤维蛋白原，纤维粘连蛋白等。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN3 PN3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） None 无 52-68 52-68 A类 Class A Huh-7-cell morphology-KCB Huh-7-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1:3传代；3-4天一次 Passage at 1: 3; and passage once in 3-4 days Huh-7 Huh-7 cell Huh-7 细胞 NICR2172 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2172 cell NICR2172 细胞 http://www.cellresource.cn/fdetail.aspx?id=2172 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Huh-7-cell morphology-KCB Huh-7-细胞形态-KCB Amelogenin:X；CSF1PO:12；D13S317:11；D16S539:9；D18S51:16；D19S433:12,14；D21S11:30；D2S1338:17,19；D3S1358:15,16；D5S818:9,11；D7S820:10,11；D8S1179:13,14；FGA:20；TH01:7；TPOX:8,11；vWA:16,19 Amelogenin:X；CSF1PO:12；D13S317:11；D16S539:9；D18S51:16；D19S433:12,14；D21S11:30；D2S1338:17,19；D3S1358:15,16；D5S818:9,11；D7S820:10,11；D8S1179:13,14；FGA:20；TH01:7；TPOX:8,11；vWA:16,19 3131C0001000700183 Human Hepatocellular Carcinoma Cells; Li-7 人肝癌细胞；Li-7 epithelial-like 上皮细胞样 adherent growth 贴壁生长 It is a human hepatoma cell line. This cell line was established from an in vitro xenograft tumor in nude mice. 人肝癌细胞株。这株细胞从裸鼠体外移植瘤中建立。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Li-7-cell morphology-SHCRC Li-7-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 Li-7 Li-7 cell Li-7 细胞 NICR1693 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1693 cell NICR1693 细胞 http://www.cellresource.cn/fdetail.aspx?id=1693 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Li-7-cell morphology-SHCRC Li-7-细胞形态-SHCRC Amelogenin:X；CSF1PO:10；D13S317:11,12；D16S539:13；D18S51:17；D21S11:30,33.2；D3S1358:15；D5S818:12；D7S820:9,11；D8S1179:13,16；FGA:19.2,25；PentaD:6,10；PentaE:10,16；TH01:7,8??；TPOX:8；vWA:15,16 Amelogenin:X；CSF1PO:10；D13S317:11,12；D16S539:13；D18S51:17；D21S11:30,33.2；D3S1358:15；D5S818:12；D7S820:9,11；D8S1179:13,16；FGA:19.2,25；PentaD:6,10；PentaE:10,16；TH01:7,8??；TPOX:8；vWA:15,16 3111C0002000000071 Human Hepatocellular Carcinoma Cells; PLC/PRF/5 人肝癌细胞；PLC/PRF/5 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell line secretes the hepatitis B virus surface antigen (HBsAg). This cell line was originally contaminated with mycoplasma and then removed with BM-cyclin. 该细胞系分泌乙肝病毒表面抗原（HBsAg）。 此细胞系原先被支原体污染，后用BM-cyclin去除支原体。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P80 P80 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A PLC-PRF-5-cell morphology-NIFDC PLC-PRF-5-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net Digestion 5-10 minutes. Passage at 1: 2. The cells can be overgrowth within 3-4 days. 消化5-10分钟。1:2。3-4天长满。 NICR443 PLC/PRF/5 PLC/PRF/5 cell PLC/PRF/5 细胞 NICR443 cell NICR443 细胞 http://www.cellresource.cn/fdetail.aspx?id=443 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf PLC-PRF-5-cell morphology-NIFDC PLC-PRF-5-细胞形态-NIFDC AMEL:X,X；CSF1PO:10,10；D12S391:15,20；D13S317:11,12；D16S539:13,13；D18S51:17,17；D19S433:11,13；D21S11:30,33.2；D2S1338:19,22；D2S441:12,14；D3S1358:15,15；D5S818:12,12；D6S1043:13,21；D7S820:9,11；D8S1179:13,16；FGA:19.2,25；Penta D:6,10；Penta E:10,16；TH01:8,8；TPOX:8, AMEL:X,X；CSF1PO:10,10；D12S391:15,20；D13S317:11,12；D16S539:13,13；D18S51:17,17；D19S433:11,13；D21S11:30,33.2；D2S1338:19,22；D2S441:12,14；D3S1358:15,15；D5S818:12,12；D6S1043:13,21；D7S820:9,11；D8S1179:13,16；FGA:19.2,25；Penta D:6,10；Penta E:10,16；TH01:8,8；TPOX:8, 3142C0001000000954 Human Hepatocellular Carcinoma Cells; PLC/PRF/5 人肝癌细胞；PLC/PRF/5 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells express Hepatitis B virus surface antigen (HBsAg)，and secrete HBsAg 表达乙型肝炎病毒表面抗原(HBsAg)，细胞分泌HBsAg MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR2191 PLC/PRF/5 PLC/PRF/5 cell PLC/PRF/5 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2191 cell NICR2191 细胞 http://www.cellresource.cn/fdetail.aspx?id=2191 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin:X；CSF1PO:10；D13S317:11,12；D16S539:13；D18S51:17；D19S433:11,13；D21S11:30,33.2；D2S1338:19,22；D3S1358:15；D5S818:12；D7S820:9,11；D8S1179:13,16；FGA:19.2,25；TH01:8；TPOX:8；vWA:15,16 Amelogenin:X；CSF1PO:10；D13S317:11,12；D16S539:13；D18S51:17；D19S433:11,13；D21S11:30,33.2；D2S1338:19,22；D3S1358:15；D5S818:12；D7S820:9,11；D8S1179:13,16；FGA:19.2,25；TH01:8；TPOX:8；vWA:15,16 3131C0001000700119 Human Hepatocellular Carcinoma Cells; PLC/PRF/5 人肝癌亚力山大细胞；PLC/PRF/5 epithelial cell 上皮细胞 adherent growth 贴壁生长 The cell line secretes the hepatitis B virus surface antigen (HBsAg). This cell line was originally contaminated with mycoplasma and then removed with BM-cyclin. 该细胞系分泌乙肝病毒表面抗原（HBsAg）。 此细胞系原先被支原体污染，后用BM-cyclin去除支原体。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(78+5) P(78+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A PLC PRF 5-cell morphology-SHCRC PLC PRF 5-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion 5-10 minutes. Passage at 1: 2. The cells can be overgrowth within 3-4 days. 消化5-10分钟。1:2。3-4天长满。 NICR687 PLC/PRF/5 PLC/PRF/5 cell PLC/PRF/5 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR687 cell NICR687 细胞 http://www.cellresource.cn/fdetail.aspx?id=687 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf PLC PRF 5-cell morphology-SHCRC PLC PRF 5-细胞形态-SHCRC Amelogenin:X；CSF1PO:12；D13S317:9；D16S539:10,11；D18S51:13,15；D19S433:14,15.2；D21S11:29；D2S1338:17,24；D3S1358:14,15；D5S818:11,12；D7S820:8,11；D8S1179:13,14；FGA:19,24；TH01:6；TPOX:8；vWA:20 Amelogenin:X；CSF1PO:12；D13S317:9；D16S539:10,11；D18S51:13,15；D19S433:14,15.2；D21S11:29；D2S1338:17,24；D3S1358:14,15；D5S818:11,12；D7S820:8,11；D8S1179:13,14；FGA:19,24；TH01:6；TPOX:8；vWA:20 3131C0001000700179 Human Cholangiocarcinoma Cells; RBE 人肝胆管癌细胞；RBE epithelial-like 上皮细胞样 adherent growth 贴壁生长 It is a human hepatobiliary carcinoma cell line. It is said that it produces CEA and CA19-9. 人肝胆管癌细胞。据说产CEA和CA19-9。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A RBE-cell morphology-SHCRC RBE-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1690 RBE RBE cell RBE 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1690 cell NICR1690 细胞 http://www.cellresource.cn/fdetail.aspx?id=1690 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RBE-cell morphology-SHCRC RBE-细胞形态-SHCRC Amelogenin:X；CSF1PO:11,12；D13S317:8,12；D16S539:12；D18S51:13,15；D21S11:29,31；D3S1358:15,16；D5S818:10,13；D7S820:8,11；D8S1179:13,14；FGA:17；PentaD:13,14；PentaE:13,21；TH01:7,9；TPOX:9；vWA:14,17 Amelogenin:X；CSF1PO:11,12；D13S317:8,12；D16S539:12；D18S51:13,15；D21S11:29,31；D3S1358:15,16；D5S818:10,13；D7S820:8,11；D8S1179:13,14；FGA:17；PentaD:13,14；PentaE:13,21；TH01:7,9；TPOX:9；vWA:14,17 3111C0002000000060 Human Liver Adenocarcinoma Cells; SK-HEP-1 人肝腹水腺癌细胞；SK-HEP-1 epithelial-like 上皮细胞样 adherent growth 贴壁生长 SK-HEP-1细胞系已被鉴定为内皮来源。 该细胞系为异倍体女性人（XX），染色体在亚三倍体范围内。 在裸鼠中，它能形成与肝癌相一致的大细胞癌。 The SK-HEP-1 cell line has been identified as an endothelial source cell line. The cell line is heteroploid (XX)of females with chromosome in the subtriploid range. In nude mice, it can form large cell carcinoma consistent with liver cancer. Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A SK-Hep-1-cell morphology-NIFDC SK-Hep-1-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net NICR432 SK-HEP-1 SK-HEP-1 cell SK-HEP-1 细胞 NICR432 cell NICR432 细胞 http://www.cellresource.cn/fdetail.aspx?id=432 SK-Hep-1-cell morphology-NIFDC SK-Hep-1-细胞形态-NIFDC Amelogenin:X；CSF1PO:11,12；D13S317:8,12；D16S539:12；D18S51:13,15；D19S433:12,15.2；D21S11:29,31；D2S1338:20,23；D3S1358:16；D5S818:10,13；D7S820:8,11；D8S1179:13,14；FGA:17；TH01:7,9；TPOX:9；vWA:14,17 Amelogenin:X；CSF1PO:11,12；D13S317:8,12；D16S539:12；D18S51:13,15；D19S433:12,15.2；D21S11:29,31；D2S1338:20,23；D3S1358:16；D5S818:10,13；D7S820:8,11；D8S1179:13,14；FGA:17；TH01:7,9；TPOX:9；vWA:14,17 3131C0001000700109 Human Liver Adenocarcinoma Cells; SK-HEP-1 人肝腹水腺癌细胞；SK-HEP-1 epithelial cell 上皮细胞 adherent growth 贴壁生长 SK-HEP-1细胞系已被鉴定为内皮来源。 该细胞系为异倍体女性人（XX），染色体在亚三倍体范围内。 在裸鼠中，它能形成与肝癌相一致的大细胞癌。 The SK-HEP-1 cell line has been identified as an endothelial source cell line. The cell line is heteroploid (XX)of females with chromosome in the subtriploid range. In nude mice, it can form large cell carcinoma consistent with liver cancer. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(22+5) P(22+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A SK-HEP-1-cell morphology-SHCRC SK-HEP-1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR677 SK-HEP-1 SK-HEP-1 cell SK-HEP-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR677 cell NICR677 细胞 http://www.cellresource.cn/fdetail.aspx?id=677 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf SK-HEP-1-cell morphology-SHCRC SK-HEP-1-细胞形态-SHCRC 3142C0001000000133 Human Liver Adenocarcinoma Cells; SK-HEP-1 人肝腹水腺癌细胞；SK-HEP-1 epithelial-like 上皮细胞样 adherent growth 贴壁生长 SK-HEP-1细胞系已被鉴定为内皮来源。 该细胞系为异倍体女性人（XX），染色体在亚三倍体范围内。 在裸鼠中，它能形成与肝癌相一致的大细胞癌。 The SK-HEP-1 cell line has been identified as an endothelial source cell line. The cell line is heteroploid (XX)of females with chromosome in the subtriploid range. In nude mice, it can form large cell carcinoma consistent with liver cancer. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C25 C25 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR901 SK-HEP-1 SK-HEP-1 cell SK-HEP-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR901 cell NICR901 细胞 http://www.cellresource.cn/fdetail.aspx?id=901 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X,Y；CSF1PO:10,12；D13S317:8,12；D16S539:10,11；D18S51:13,21；D19S433:12,12.2；D21S11:29,30；D2S1338:18；D3S1358:14,17；D5S818:8,11；D7S820:11,12；D8S1179:10,11；FGA:19,24；TH01:7,8；TPOX:8；vWA:14,17 Amelogenin:X,Y；CSF1PO:10,12；D13S317:8,12；D16S539:10,11；D18S51:13,21；D19S433:12,12.2；D21S11:29,30；D2S1338:18；D3S1358:14,17；D5S818:8,11；D7S820:11,12；D8S1179:10,11；FGA:19,24；TH01:7,8；TPOX:8；vWA:14,17 3131C0001000700181 Human Hepatoblastoma Cells; HuH-6 人肝母细胞瘤细胞；HuH-6 epithelial-like 上皮细胞样 adherent growth 贴壁生长 It is reported that the cells can produce Globulin and alpha-fetoprotein. 据报道产球蛋白和甲胎蛋白。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A HuH-6-cell morphology-SHCRC HuH-6-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 6-7 days. 消化3-5分钟。1:2。6-7天长满。 HuH-6 HuH-6 cell HuH-6 细胞 NICR1691 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1691 cell NICR1691 细胞 http://www.cellresource.cn/fdetail.aspx?id=1691 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf HuH-6-cell morphology-SHCRC HuH-6-细胞形态-SHCRC Amelogenin:X,Y；CSF1PO:10,12；D13S317:8,12；D16S539:10,11；D18S51:13,21；D19S433:12,12.2；D21S11:29,30；D2S1338:18；D3S1358:14,17；D5S818:8,11；D7S820:11,12；D8S1179:10,11；FGA:19,24；TH01:7,8；TPOX:8；vWA:14,17 Amelogenin:X,Y；CSF1PO:10,12；D13S317:8,12；D16S539:10,11；D18S51:13,21；D19S433:12,12.2；D21S11:29,30；D2S1338:18；D3S1358:14,17；D5S818:8,11；D7S820:11,12；D8S1179:10,11；FGA:19,24；TH01:7,8；TPOX:8；vWA:14,17 3111C0001CCC000477 Human Hepatoblastoma Cells; HuH-6 人肝母细胞瘤细胞；HuH-6 epithelium-like; polygonal shape 上皮样；多角形 adherent growth 贴壁生长 It is reported that the cells can produce Globulin and alpha-fetoprotein. 据报道产球蛋白和甲胎蛋白。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代；6-7天传代一次。 Passage at 1: 2; and passage once in 6-7 days. HuH-6 HuH-6 cell HuH-6 细胞 NICR2150 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2150 cell NICR2150 细胞 http://www.cellresource.cn/fdetail.aspx?id=2150 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000200040 Immortalized Human Liver Cells; THLE-3 人肝永生化细胞；THLE-3 epithelial cell 上皮细胞 adherent growth 贴壁生长 THLE-2 (ATCC CRL-10149 和 THLE-3 (ATCC CRL-11233)细胞系是正常肝细胞用SV40大T抗原转染得到。 将含有SV40 T抗原的Bgl I-Hpa I片段的逆转录病毒载体导入兼嗜性包装细胞株PA317中生产病毒。 THLE-2 and THLE-3表达正常成人肝上皮细胞特征性的表型。 当注射到无胸腺裸鼠中时，它们不成瘤，具有接近二倍体的核型，不表达甲胎蛋白。 THLE-2 and THLE-3将benzo[a]pyrene, N-nitrosodimet The THLE-2 (ATCC CRL-10149) and THLE-3 (ATCC CRL-11233) cell lines were obtained by transfection of normal hepatocytes with SV40 large T antigen. Retroviral vectors containing the Bgl I-Hpa I fragment of SV40 T antigen were introduced into a amphophilic packaging cell line PA317 to produce the virus. THLE-2 and THLE-3 express the characteristic phenotype of normal adult liver epithelial cells. When injected into thymic-free nude mice, they do not form tumors, with a karyotype close to diploid, and do not express alpha-fetoprotein. THLE-2 and THLE-3 metabolize benzo [a] pyrene, N-nitrosodimethylamine, and aflatoxin B1 into the final oncogenic metabolites attached to DNA, thus revealing the role of the cytochrome P450 pathway. THLE also retains other enzymes that are involved in chemical oncolytic metabolism, such as epoxide hydrolase, NADPH cytochrome P450 reductase, superoxide dismutase, catalase, glutathione S-transferases, and glutathione peroxidase. These immortalized human hepatocytes make up the study drug toxicology and Etiological and pathological models of human hepatocellular carcinoma in vitro. Cultures submitted to the ATCC in January 1993 were contaminated Mycoplasma. Their offspring were treated with mycoplasma reagents for 21 days. Six weeks after the treatment, Mycoplasma was tested using Hoechst staining, PCR and standard culture tests. The results were negative. Clonetics Corporation, Walkersville, MD 21793 (cat # CC3170). The kit contains 500 milliliters of the basic medium and the separately frozen additives. We do not use GA and Epinephrine but add 5 ng/ml EGF and 70 ng/ml Phosphoethanolamine. Clonetics Corporation, Walkersville, MD 21793的BEGM培养基(目录号CC3170)。 套装包含了500毫升基本培养基及分别冻存的添加剂，我们不用其中的GA和Epinephrine但另外添加了5 ng/ml EGF, 70 ng/ml Phosphoethanolamine。 PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1661 THLE-3 THLE-3 cell THLE-3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1661 cell NICR1661 细胞 http://www.cellresource.cn/fdetail.aspx?id=1661 Amelogenin：X；CSF1PO：12；D13S317：13；D16S539：13，14；D18S51：15，18；D19S433：11，13，14；D21S11：29，30，31；D2S1338：23，25；D3S1358：16；D5S818：11，12；D7S820：10；D8S1179：10，14；FGA：21，26；TH01：7，8；TPOX：9；vWA：18，20； Amelogenin：X；CSF1PO：12；D13S317：13；D16S539：13，14；D18S51：15，18；D19S433：11，13，14；D21S11：29，30，31；D2S1338：23，25；D3S1358：16；D5S818：11，12；D7S820：10；D8S1179：10，14；FGA：21，26；TH01：7，8；TPOX：9；vWA：18，20； 3111C0001CCC000172 Human Cervical Carcinoma Cells; C-33A 人宫颈癌细胞；C-33A epithelioid 上皮样 adherent growth 贴壁生长 This cell line was established by Auerperg N and was derived from a cervical carcinoma biopsy specimen. P53 +, pRB +, point mutation ocurrs at codon 273. Papillomavirus DNA and RNA detections were negative. 该细胞系由Auersperg N建立，源于宫颈癌活检标本。p53 +，pRB +，在273位密码子发生点突变。乳头状瘤病毒DNA、RNA阴性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P101 P101 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 46,XX,there are multiple derived chromosomes 46,XX,有多条衍生染色体 A类 Class A C33A-Cell Morphology-PUMC C33A-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:8传代；2~3天换液1次。 Passage at 1: 3 ~ 1: 8; and change fluid once in 2~3 days. C-33A C-33A cell C-33A 细胞 NICR172 PMID: 9187105 PMID: 9187105 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR172 cell NICR172 细胞 http://www.cellresource.cn/fdetail.aspx?id=172 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf C33A-Cell Morphology-PUMC C33A-细胞形态-PUMC 3142C0001000000132 Human Cervical Carcinoma Cells; C-33A 人宫颈癌细胞；C-33A epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line was established by Auerperg N and was derived from a cervical carcinoma biopsy specimen. P53 +, pRB +, point mutation ocurrs at codon 273. Papillomavirus DNA and RNA detections were negative. 该细胞系由Auersperg N建立，源于宫颈癌活检标本。p53 +，pRB +，在273位密码子发生点突变。乳头状瘤病毒DNA、RNA阴性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C97 C97 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. C-33A C-33A cell C-33A 细胞 NICR900 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR900 cell NICR900 细胞 http://www.cellresource.cn/fdetail.aspx?id=900 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin:X；CSF1PO:12；D13S317:13；D16S539:13,14；D18S51:15,18；D21S11:29,30,31；D3S1358:16；D5S818:11,12；D7S820:10；D8S1179:10,14；FGA:21,26；PentaD:10；PentaE:6,8；TH01:7,8；TPOX:9；vWA:18,20 Amelogenin:X；CSF1PO:12；D13S317:13；D16S539:13,14；D18S51:15,18；D21S11:29,30,31；D3S1358:16；D5S818:11,12；D7S820:10；D8S1179:10,14；FGA:21,26；PentaD:10；PentaE:6,8；TH01:7,8；TPOX:9；vWA:18,20 3111C0002000000091 Human Cervical Carcinoma Cells; C-33A 人宫颈癌细胞；C-33A epithelioid 上皮样 adherent growth 贴壁生长 The cell carries the oncogenic gene p53 +, pRB + and is tumorigenic in nude mice. 该细胞携带有致癌基因p53+，pRB+；在裸鼠上致瘤。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P100 P100 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A C-33A-cell morphology-NIFDC C-33A-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:3-1:8，每周换液2次 Passage at 1: 3-1: 8, and change fluid for 2 times a week C-33A C-33A cell C-33A 细胞 NICR2116 NICR2116 cell NICR2116 细胞 http://www.cellresource.cn/fdetail.aspx?id=2116 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf C-33A-cell morphology-NIFDC C-33A-细胞形态-NIFDC Amelogenin：X；CSF1PO：9，10；D13S317：12，13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：18，21；TH01：7；TPOX：8，12；vWA：16，18； Amelogenin：X；CSF1PO：9，10；D13S317：12，13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：18，21；TH01：7；TPOX：8，12；vWA：16，18； 3111C0001CCC000344 Human Cervical Carcinoma Cells; H1HeLa 人宫颈癌细胞；H1HeLa epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells was established by V. Hamparian from HeLa cells and is sensitive to rhinovirus and used for passage and titration of rhinovirus. 该细胞由V. Hamparian从HeLa细胞建立，对鼻病毒敏感，用于鼻病毒的传代及滴定。 L15: Leibovitz Medium 10%FBS L15: Leibovitz Medium 10%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P19 P19 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4～1:10传代，每周换液2～3次 Passage at 1: 4 ~ 1:10, and change fluid for 2 to 3 times a week H1HeLa H1HeLa cell H1HeLa 细胞 NICR1533 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1533 cell NICR1533 细胞 http://www.cellresource.cn/fdetail.aspx?id=1533 L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf Amelogenin：X；CSF1PO：9，10；D13S317：13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：18，21；TH01：7；TPOX：8，12；vWA：16，18； Amelogenin：X；CSF1PO：9，10；D13S317：13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：18，21；TH01：7；TPOX：8，12；vWA：16，18； 3111C0001CCC000188 Human Cervical Carcinoma Cells; Hela S3 [HeLaS3] 人宫颈癌细胞；Hela S3 [HeLaS3] epithelioid 上皮样 adherent growth 贴壁生长 The cells were constructed in 1955 by Buck TT, Marcus PI and Cieciura SJ, containing HPV-18 sequences; the cells were keratin-positive, and can be used in mammalian cell clones analysis related to chromosomal mutation, cell nutrition, colony formation. 该细胞是1955年由Puck TT, Marcus PI和Cieciura SJ建系的，含HPV-18序列；角蛋白阳性；可用于与染色体突变、细胞营养、集落形成相关的哺乳动物细胞的克隆分析。 F12: Ham＇s F12 Nutrient Mixture 10%FBS F12: Ham＇s F12 Nutrient Mixture 10%FBS http://www.cellresource.cn/pdf/20080108100810.pdf http://www.cellresource.cn/pdf/20080108100810.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 56~66 56~66 A类 Class A HeLa S3-Cell Morphology-PUMC HeLa S3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:10传代；2~3天换液1次。 Passage at 1: 4 ~ 1:10; and change fluid once in 2~3 days. HeLaS3 HeLaS3 cell HeLaS3 细胞 Hela S3 Hela S3 cell Hela S3 细胞 NICR188 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR188 cell NICR188 细胞 http://www.cellresource.cn/fdetail.aspx?id=188 F12: Ham＇s F12 Nutrient Mixture 10%FBS URI: http://www.cellresource.cn/pdf/20080108100810.pdf F12: Ham＇s F12 Nutrient Mixture 10%FBS URI: http://www.cellresource.cn/pdf/20080108100810.pdf HeLa S3-Cell Morphology-PUMC HeLa S3-细胞形态-PUMC Amelogenin:X；CSF1PO:9,10；D13S317:13.3；D16S539:9,10；D18S51:16；D21S11:27,28；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；PentaD:8,15；PentaE:7,17；TH01:7；TPOX:8,12；vWA:16,18 Amelogenin:X；CSF1PO:9,10；D13S317:13.3；D16S539:9,10；D18S51:16；D21S11:27,28；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；PentaD:8,15；PentaE:7,17；TH01:7；TPOX:8,12；vWA:16,18 3111C0002000000009 Human Cervical Carcinoma Cells; Hela S3 [HeLaS3] 人宫颈癌细胞；Hela S3 [HeLaS3] epithelioid 上皮样 adherent growth 贴壁生长 The cells were constructed in 1955 by Buck TT, Marcus PI and Cieciura SJ, containing HPV-18 sequences; the cells were keratin-positive, and can be used in mammalian cell clones analysis related to chromosomal mutation, cell nutrition, colony formation. 该细胞是1955年由Puck TT, Marcus PI和Cieciura SJ建系的，含HPV-18序列；角蛋白阳性；可用于与染色体突变、细胞营养、集落形成相关的哺乳动物细胞的克隆分析。 F12K new-born calf serum，10% F12K 新生牛血清，10% F12K 新生牛血清，10% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Hela S3-cell morphology-NIFDC Hela S3-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:4-1:10 Passage at 1: 4-1: 10 HeLaS3 HeLaS3 cell HeLaS3 细胞 Hela S3 Hela S3 cell Hela S3 细胞 NICR381 NICR381 cell NICR381 细胞 http://www.cellresource.cn/fdetail.aspx?id=381 F12K 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf Hela S3-cell morphology-NIFDC Hela S3-细胞形态-NIFDC 3111C0002000000092 Human Cervical Carcinoma Cells; SiHa 人宫颈癌细胞；SiHa epithelioid 上皮样 adherent growth 贴壁生长 The cells carry the oncogenes p53 +, pRB + and contain the integrated HPV16 virus. 该细胞携带有致癌基因p53+，pRB+；包含有整合的HPV16型病毒。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf No cell information 无该细胞资料 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A SiHa-cell morphology-NIFDC SiHa-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:3-1:8，每周换液2次 Passage at 1: 3-1: 8, and change fluid for 2 times a week NICR2117 SiHa SiHa cell SiHa 细胞 NICR2117 cell NICR2117 细胞 http://www.cellresource.cn/fdetail.aspx?id=2117 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf SiHa-cell morphology-NIFDC SiHa-细胞形态-NIFDC Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：10，13；D18S51：14；D19S433：13；D21S11：31.2，32.2；D2S1338：24，25；D3S1358：15；D5S818：13；D7S820：11，12；D8S1179：14；FGA：24；TH01：6；TPOX：8，11；vWA：18； Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：10，13；D18S51：14；D19S433：13；D21S11：31.2，32.2；D2S1338：24，25；D3S1358：15；D5S818：13；D7S820：11，12；D8S1179：14；FGA：24；TH01：6；TPOX：8，11；vWA：18； 3111C0001CCC000038 Human Osteosarcoma Cells; HOS 人骨肉瘤细胞；HOS epithelioid-like and fibroblast- like cell 上皮样细胞和成纤维样细胞均有 adherent growth 贴壁生长 The cells were isolated from the osteosarcoma tissue of a 13-year-old white female and expressed in flat form, low saturation density, low clonal formation rate in soft agar, and susceptibility to compounds and viral infections. 该细胞是从一名13岁的白人女性的骨肉瘤组织中分离建立的，表现为扁平形态、低饱和密度、软琼脂中的低克隆形成率和对化合物及病毒感染的敏感性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P35 P35 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 46~50 46~50 A类 Class A HOS-Cell Morphology-PUMC HOS-Species Identification-PUMC HOS-cell morphology-PUMC HOS-物种鉴定-PUMC HOS-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；每周2~3次。 Passage at 1: 2 ~ 1: 4; and passage for 2 to 3 times per week. HOS HOS cell HOS 细胞 NICR38 PMID: 8550604 PMID: 8550604 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR38 cell NICR38 细胞 http://www.cellresource.cn/fdetail.aspx?id=38 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf HOS-Cell Morphology-PUMC HOS-Species Identification-PUMC HOS-cell morphology-PUMC HOS-物种鉴定-PUMC HOS-细胞形态-PUMC HOS-细胞形态-PUMC STR Identification STR鉴定 3142C0001000000359 Human Osteosarcoma Cells; HOS 人骨肉瘤细胞；HOS epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from the osteosarcoma tissue of a 13-year-old white girl, established by J.S. Rhim. The cell is flat shape, with low saturation density, and the plate-out rate is low in soft agar culture. The chromosome number of this cell is hyperdiploid, the majority is 49 (26%). 该细胞源自一位13岁白人女孩的骨肉瘤组织，由J.S. Rhim建系。该细胞形状扁平，饱和密度低，在软琼脂培养中出板率低。该细胞的染色体数为超二倍体，主流为49条（26%）。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C8 C8 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HOS HOS cell HOS 细胞 NICR2010 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2010 cell NICR2010 细胞 http://www.cellresource.cn/fdetail.aspx?id=2010 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf AMEL:X,X；CSF1PO:12,12；D12S391:20,20；D13S317:12,12；D16S539:10,13；D18S51:14,14；D19S433:13,13；D21S11:31.2,31.2；D2S1338:24,25；D2S441:10,10；D3S1358:15,15；D5S818:13,13；D6S1043:18,18；D7S820:11,12；D8S1179:11,14；FGA:24,24；Penta D:9,10；Penta E:7,12；TH01:6,6；TPOX:8, AMEL:X,X；CSF1PO:12,12；D12S391:20,20；D13S317:12,12；D16S539:10,13；D18S51:14,14；D19S433:13,13；D21S11:31.2,31.2；D2S1338:24,25；D2S441:10,10；D3S1358:15,15；D5S818:13,13；D6S1043:18,18；D7S820:11,12；D8S1179:11,14；FGA:24,24；Penta D:9,10；Penta E:7,12；TH01:6,6；TPOX:8, 3142C0001000000956 Human Osteosarcoma Cells; HOS 人骨肉瘤细胞；HOS epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells show high saturation density with high plating efficiency on soft agar, and are tumorigenicity in nude mice. 细胞表现出高的饱和密度，在软琼脂上高电镀效率在裸鼠体内致瘤性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HOS HOS cell HOS 细胞 NICR2193 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2193 cell NICR2193 细胞 http://www.cellresource.cn/fdetail.aspx?id=2193 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000700128 Human Osteosarcoma Cells; SW 1353 人骨肉瘤细胞；SW 1353 fibroblasts 成纤维细胞 adherent growth 贴壁生长 SW 1353 cells were initially obtained from primary grade II osteosarcoma of the right humerus in a 72-year-old white female. The initial medium was L-15 containing cortisone and insulin with 10% FBS and antibiotics addition. In January 1982 ATCC received 1 cryopreserved tube of cells of 12nd generations. SW 1353细胞最初从一位72岁女性白人的右肱骨原发性II级骨肉瘤中得到。 最初的培养基是含有可的松和胰岛素的L-15添加10%FBS和抗生素。 1982年１月ATCC收到１支传代至12代的冻存管。 L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P(25+5) P(25+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A SW1353-cell morphology-SHCRC SW1353-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR696 SW 1353 SW 1353 cell SW 1353 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR696 cell NICR696 细胞 http://www.cellresource.cn/fdetail.aspx?id=696 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf SW1353-cell morphology-SHCRC SW1353-细胞形态-SHCRC Amelogenin：X；CSF1PO：13；D13S317：13；D16S539：11，12；D18S51：10.2，12，14；D19S433：15；D21S11：31；D2S1338：20，24；D3S1358：16，18.3；D5S818：11；D7S820：11，12；D8S1179：12，14；FGA：20；TH01：6，9.3；TPOX：11，12；vWA：14，18； Amelogenin：X；CSF1PO：13；D13S317：13；D16S539：11，12；D18S51：10.2，12，14；D19S433：15；D21S11：31；D2S1338：20，24；D3S1358：16，18.3；D5S818：11；D7S820：11，12；D8S1179：12，14；FGA：20；TH01：6，9.3；TPOX：11，12；vWA：14，18； 3111C0001CCC000028 Human Osteosarcoma Cells; U-2 OS [U2OS; U2-OS; U-2OS] 人骨肉瘤细胞；U-2 OS [U2OS；U2-OS；U-2OS] epithelioid 上皮样 adherent growth 贴壁生长 This cell line (formerly known as 2T) was established and isolated by Ponten J and Saksela E from the moderately differentiated sarcoma of the tibia of a 15-year-old girl in 1964. No virus detected when co-cultured with WI-38 or complement fixation assay (CF method) against monkey vacuolar virus SV40, respiratory syncytial virus RSV or adenovirus. The cells expressed insulin-like growth factor Ⅰ, Ⅱ (IGF-Ⅰ, IGF-Ⅱ) receptor and osteosarcoma-derived growth factor (ODGF). 该细胞系(原名2T)是由Ponten J和Saksela E在1964年从一名15岁女孩的胫骨中等分化的肉瘤中分离建立的。与WI-38共培养或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的补体结合试验（CF法）未检测到病毒。该细胞表达胰岛素样生长因子Ⅰ、Ⅱ（IGF-Ⅰ、IGF-Ⅱ）受体和骨肉瘤衍生生长因子（ODGF）。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P45 P45 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A U2OS-Species Identification-PUMC U2OS-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；每周2~3次。　 Passage at 1: 3 ~ 1: 6; and passage for 2 to 3 times a week. NICR28 U-2 OS U-2 OS cell U-2 OS 细胞 U-2OS U-2OS cell U-2OS 细胞 U2-OS U2-OS cell U2-OS 细胞 U2OS U2OS cell U2OS 细胞 PMID: 9270029 PMID: 9270029 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR28 cell NICR28 细胞 http://www.cellresource.cn/fdetail.aspx?id=28 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf U2OS-Species Identification-PUMC U2OS-物种鉴定-PUMC Amelogenin:X；CSF1PO:13；D13S317:13；D16S539:11,12；D18S51:14；D19S433:15；D21S11:31；D2S1338:20,24；D3S1358:16；D5S818:11；D7S820:11,12；D8S1179:12,14；FGA:20；TH01:6,9.3；TPOX:11,12；vWA:14,18 Amelogenin:X；CSF1PO:13；D13S317:13；D16S539:11,12；D18S51:14；D19S433:15；D21S11:31；D2S1338:20,24；D3S1358:16；D5S818:11；D7S820:11,12；D8S1179:12,14；FGA:20；TH01:6,9.3；TPOX:11,12；vWA:14,18 3131C0001000700088 Human Osteosarcoma Cells; U-2 OS [U2OS; U2-OS; U-2OS] 人骨肉瘤细胞；U-2 OS [U2OS；U2-OS；U-2OS] epithelial cell 上皮细胞 adherent growth 贴壁生长 J. Ponten and E. this cell (originally named as 2T) is derived from a moderately differentiated sarcoma of the tibia in a 15-year-old girl. No virus was detected when co-culture with WI-38 or CF test for SV40, RSV or adenovirus. Mycoplasma contamination was discovered and removed in 1972. J. Ponten and E. 这株细胞(原名2T)源自15岁女孩的胫骨中等分化的肉瘤。 与WI-38共培养或CF检测SV40，RSV或腺病毒时未检测到病毒。 1972年发现支原体污染并去除。 RPMI 1640 (w/o Hepes) heat-inactivatedhorse serum，10% qualified fetal bovine serum，5% RPMI 1640 (w/o Hepes) 热灭活马血清，10%；优质胎牛血清，5% RPMI 1640 (w/o Hepes) 热灭活马血清，10%；优质胎牛血清，5% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A LDH检测报告U-2 OS-Isoenzyme-SHCRC LDH检测报告U-2 OS-同工酶-SHCRC U-2 OS-cell morphology-SHCRC U-2 OS-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR656 U-2 OS U-2 OS cell U-2 OS 细胞 U-2OS U-2OS cell U-2OS 细胞 U2-OS U2-OS cell U2-OS 细胞 U2OS U2OS cell U2OS 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR656 cell NICR656 细胞 http://www.cellresource.cn/fdetail.aspx?id=656 RPMI 1640 (w/o Hepes) 热灭活马血清，10%；优质胎牛血清，5% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 热灭活马血清，10%；优质胎牛血清，5% URI: http://www.cellresource.cn/pdf/20080108101040.pdf LDH检测报告U-2 OS-Isoenzyme-SHCRC LDH检测报告U-2 OS-同工酶-SHCRC U-2 OS-cell morphology-SHCRC U-2 OS-细胞形态-SHCRC 3142C0001000000326 Human Myeloma Cell line; NCI-H929 人骨髓瘤细胞；NCI-H929 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells were deposited by College of Life Sciences, Wuhan University. NCI-H929 cell is a human myeloma cell, mainly used in human myeloma disease research and so on. 该细胞由武汉大学生命科学学院提交保藏。NCI-H929细胞是一株人骨髓瘤细胞，主要用于人骨髓瘤疾病研究等。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C7 C7 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NCI-H929 NCI-H929 cell NCI-H929 细胞 NICR1977 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1977 cell NICR1977 细胞 http://www.cellresource.cn/fdetail.aspx?id=1977 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：10，13；D13S317：11，12；D16S539：11，12；D18S51：17，18；D19S433：12，13；D21S11：28，30；D2S1338：17，22；D3S1358：14，17；D5S818：12；D7S820：8，10；D8S1179：13，15；FGA：21；TH01：8，9.3；TPOX：8，9；vWA：15，17； Amelogenin：X；CSF1PO：10，13；D13S317：11，12；D16S539：11，12；D18S51：17，18；D19S433：12，13；D21S11：28，30；D2S1338：17，22；D3S1358：14，17；D5S818：12；D7S820：8，10；D8S1179：13，15；FGA：21；TH01：8，9.3；TPOX：8，9；vWA：15，17； 3111C0001CCC000031 Human Rhabdomyosarcoma Cells; A-204 人横纹肌肉瘤细胞；A-204 epithelioid 上皮样 adherent growth 贴壁生长 The cells can form tumors in the nude mice. 在裸鼠中成瘤。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） XX，hypodiploid XX，亚二倍体 A类 Class A A204-Species Identification-PUMC A204-cell morphology-PUMC A204-物种鉴定-PUMC A204-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:6~1:10传代；每周2~3次。 Passage at 1: 6 ~ 1:10; and passage for 2 to 3 times per week. A-204 A-204 cell A-204 细胞 NICR31 PMID: 15913453 PMID: 15913453 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR31 cell NICR31 细胞 http://www.cellresource.cn/fdetail.aspx?id=31 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf A204-Species Identification-PUMC A204-cell morphology-PUMC A204-物种鉴定-PUMC A204-细胞形态-PUMC STR Identification STR鉴定 3142C0001000000336 Human Rhabdomyosarcoma Cells; A-204 人横纹肌肉瘤细胞；A-204 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell line is derived from the muscle tissue of a 1-year-old girl with rhabdomyosarcoma, established by D.J. Giard. 该细胞系源自一位患有横纹肌肉瘤的1岁女孩的肌肉组织，由D.J. Giard建立。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C6 C6 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. A-204 A-204 cell A-204 细胞 NICR1987 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1987 cell NICR1987 细胞 http://www.cellresource.cn/fdetail.aspx?id=1987 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf 3111C0002000000105 Human Rhabdomyosarcoma Cells; A-204 人横纹肌肉瘤细胞；A-204 epithelioid 上皮样 adherent 贴壁 The cells are non-tumorigenic in nude mice and of diploidy or tetraploidy. 该细胞在裸鼠上无致瘤性，呈二倍性或四倍性。 10%FBS 10%FBS 90%FBS+10%DMSO 90%FBS+10%DMSO Culture Method（-） 培养法（-） A类 Class A A-204-cell morphology-NIFDC A-204-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:6-1:10，每周换液2-3次 Passage at 1: 6-1: 10, and change fluid for 2-3 times a week A-204 A-204 cell A-204 细胞 NICR2128 NICR2128 cell NICR2128 细胞 http://www.cellresource.cn/fdetail.aspx?id=2128 A-204-cell morphology-NIFDC A-204-细胞形态-NIFDC Amelogenin:X；CSF1PO:11,12；D13S317:8,13；D16S539:11；D18S51:13,16；D19S433:13,14；D21S11:29,30.2；D2S1338:16,21；D3S1358:14；D5S818:11,12；D7S820:10,12；D8S1179:11,13；FGA:19,20；TH01:9.3；TPOX:8；vWA:15,18 Amelogenin:X；CSF1PO:11,12；D13S317:8,13；D16S539:11；D18S51:13,16；D19S433:13,14；D21S11:29,30.2；D2S1338:16,21；D3S1358:14；D5S818:11,12；D7S820:10,12；D8S1179:11,13；FGA:19,20；TH01:9.3；TPOX:8；vWA:15,18 3131C0001000700006 Human Rhabdomyosarcoma Cells; A-673 [A673] 人横纹肌肉瘤细胞；A-673 [A673] fibroblasts 成纤维细胞 adherent growth 贴壁生长 A-673 is a rhabdomyosarcoma derived from 15-year-old women. It forms clones on soft agar and forms tumors in mice treated with immunosuppressed serum. The cells have more than four marker chromosomes, including an extra F chromosome and two abnormal B chromosomes. A-673是源自15岁女性的横纹肌肉瘤。 它在软琼脂上形成克隆，在免疫抑制血清处理的小鼠中成瘤。 有四个以上的标志染色体，一个额外的F染色体和两个异常的B染色体。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A A-673-cell morpholgoy-SHCRC A-673-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 A-673 A-673 cell A-673 细胞 A673 A673 cell A673 细胞 NICR577 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR577 cell NICR577 细胞 http://www.cellresource.cn/fdetail.aspx?id=577 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf A-673-cell morpholgoy-SHCRC A-673-细胞形态-SHCRC 3142C0001000000321 Human Rhabdomyosarcoma Cells; RD 人横纹肌肉瘤细胞；RD epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were deposited by Professor Wu Jianguo, College of Life Sciences, Wuhan University. RD cells can be used for basic study and clinical study of human rhabdomyosarcoma. 该细胞由武汉大学生命科学学院吴建国教授提交保藏。RD细胞可用于人横纹肌肉瘤的基础研究和临床研究。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C32 C32 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR1972 RD RD cell RD 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1972 cell NICR1972 细胞 http://www.cellresource.cn/fdetail.aspx?id=1972 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：10，11；D13S317：13；D16S539：10，11；D18S51：13；D19S433：11，14；D21S11：28，29；D2S1338：23；D3S1358：15，17；D5S818：10，11；D7S820：8，12；D8S1179：11，15；FGA：20，21；TH01：9.3；TPOX：9；vWA：18； Amelogenin：X；CSF1PO：10，11；D13S317：13；D16S539：10，11；D18S51：13；D19S433：11，14；D21S11：28，29；D2S1338：23；D3S1358：15，17；D5S818：10，11；D7S820：8，12；D8S1179：11，15；FGA：20，21；TH01：9.3；TPOX：9；vWA：18； 3111C0001CCC000266 Human Rhabdomyosarcoma Cells; TE671 Subline No.2 人横纹肌肉瘤细胞；TE671 Subline No.2 epithelioid 上皮样 adherent growth 贴壁生长 This cell expresses the receptors for N-type acetylcholine and peripheral benzodiazepines. 该细胞表达N型乙酰胆碱和外周型苯二氮?的受体。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 78~90 78~90 A类 Class A TE671 Subline No.2-Species Identification-PUMC TE671 Subline No.2-物种鉴定-PUMC TE671 Subline No2-cell morphology-PUMC TE671 Subline No2-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代，2~3天换液1次。 Passage at 1: 3 ~ 1: 6, and change fluid once in 2~3 days. NICR266 TE671 Subline No.2 TE671 Subline No.2 cell TE671 Subline No.2 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR266 cell NICR266 细胞 http://www.cellresource.cn/fdetail.aspx?id=266 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf TE671 Subline No.2-Species Identification-PUMC TE671 Subline No.2-物种鉴定-PUMC TE671 Subline No2-cell morphology-PUMC TE671 Subline No2-细胞形态-PUMC Amelogenin:X；CSF1PO:10,11；D13S317:9,11；D16S539:11；D18S51:12,16；D19S433:11,13；D21S11:29,30.2,31.2；D2S1338:18,19；D3S1358:15；D5S818:11；D7S820:7；D8S1179:13,15；FGA:21,22,23；TH01:7；TPOX:11；vWA:14,17 Amelogenin:X；CSF1PO:10,11；D13S317:9,11；D16S539:11；D18S51:12,16；D19S433:11,13；D21S11:29,30.2,31.2；D2S1338:18,19；D3S1358:15；D5S818:11；D7S820:7；D8S1179:13,15；FGA:21,22,23；TH01:7；TPOX:11；vWA:14,17 3131C0001000700071 Human Erythroleukemic Cells; HEL 人红白细胞白血病细胞；HEL lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This lymphoblast-like cell line, derived from a 30-year-old white male with malignant erythroleukemia, is naturally producing and can induce globulin synthesis. The cells were negative for Epstein-Barr virus nuclear antigen test and had no surface immunoglobulin and cytoplasmic immunoglobulin. HEL cells express HLA antigens (HLA-A3, AW32, BW35), β-2 microglobulin, and a certain proportion of cells also express Ia antigen. This cell line provides a model for studying red cell differentiation and globulin gene expression. It is similar to hemophilia in mice. 这株淋巴母细胞样细胞株，源自一位30岁白人男性一，患有恶性红细胞白血病，能够自然产生并能诱导球蛋白合成。 细胞的EB病毒核抗原阴性，没有表面免疫球蛋白与细胞质免疫球蛋白。 HEL细胞表达HLA抗原(HLA-A3, AW32, BW35), β-2 小球蛋白，一定比例的细胞还表达Ia抗原。 这个细胞株提供了一种用于研究红细胞分化和球蛋白基因表达的模型。 它类似于小鼠中的血友病。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. HEL HEL cell HEL 细胞 NICR639 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR639 cell NICR639 细胞 http://www.cellresource.cn/fdetail.aspx?id=639 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700202 Human Erythroleukemia Cells; Kasumi-1 人红白血病细胞；Kasumi-1 myeloblast 原粒细胞 suspension growth 悬浮生长 This is a leukemia cell line with translocation of chromosome 8: 21. This translocation causes the link of AML1 gene wih the ETO (or MTG8) gene, increasing the expression of the fusion gene AML1-ETO (also known as AML1-MTG or RUNX1-CBF2T1), so the cells produce AML1-ETO protein. This protein downregulates the binding activity of CEBPA mRNA, protein and DNA, which is extremely important for the differentiation of granulocytes. The cell line is established in the peripheral blood of an acute leukemia patient. This cell line is positive for myeloperoxidase staining and shows the morphology of myeloid maturation. Proliferation tests showed that the cultured cells responded to IL-3, IL-6, G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte-macrophage colony stimulating factor), but did not respond to IL-1 and IL-5. Dimethylsulfoxide, G-CSF and IL-5 were added to the culture in vitro, and no maturation of granulated or eosinophilic cells was observed. The inducible macrophage like cells can be seen with the addition of phorbol ester. 1,25S- (OH) 2-16 and 23-diene-26-F3-10-nor D3 inhibit its proliferation. 这是一个带有8：21号染色体转位的白血病细胞株。 这个转位使得AML1基因和ETO (或称 MTG8)基因串联，使融合基因AML1-ETO (也称作 AML1-MTG或 RUNX1-CBF2T1)的表达升高，因而细胞产生嵌合的AML1-ETO蛋白。 这个蛋白下调CEBPA mRNA，蛋白和DNA的结合活性，而这种结合对粒性白细胞的分化是极端重要的。 这株细胞建立于一位急性白血病患者的外周血。 这株细胞髓过氧化物酶阳性，显示其髓性成熟的形态。 增生试验显示，培养的细胞对IL-3、IL-6、G-CSF（粒细胞集落刺激因子）、GM-CSF（粒细胞-巨噬细胞集落刺激因子）有响应，但对IL-1和IL-5没有响应。在体外液体培养中分别加入二甲亚砜、G-CSF、IL-5，也没有观察到粒性或嗜酸性细胞的成熟。 加入佛波酯可以看到诱导出的巨噬细胞样细胞。 1,25S-(OH)2-16,23-diene-26-F3-10-nor D3抑制其增生。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，20% RPMI 1640 (w/o Hepes) 优质胎牛血清，20% RPMI 1640 (w/o Hepes) 优质胎牛血清，20% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. Kasumi-1 Kasumi-1 cell Kasumi-1 细胞 NICR1709 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1709 cell NICR1709 细胞 http://www.cellresource.cn/fdetail.aspx?id=1709 RPMI 1640 (w/o Hepes) 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X，Y；CSF1PO：12，13；D13S317：8，9；D16S539：9，12；D18S51：13；D19S433：14，16；D21S11：30；D2S1338：19；D3S1358：15；D5S818：13；D7S820：12；D8S1179：11，15；FGA：18，19；TH01：7，9；TPOX：8；vWA：15，17； Amelogenin：X，Y；CSF1PO：12，13；D13S317：8，9；D16S539：9，12；D18S51：13；D19S433：14，16；D21S11：30；D2S1338：19；D3S1358：15；D5S818：13；D7S820：12；D8S1179：11，15；FGA：18，19；TH01：7，9；TPOX：8；vWA：15，17； 3111C0001CCC000118 Human Erythroleukemia Cells; TF-1 人红系白血病细胞；TF-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This cell line was established in 1987 by Kitamura T et al., and was derived from a heparinized bone marrow sample from a 35-year-old Japanese male with severe pancytopenia. Cell growth was entirely dependent on IL-3 or GM-CSF and did not respond to IL-5. Various lymphocytes and cytokines have effects on the cells, such as IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, CSF, LIF, and NGF. The cells did not express glycoprotein A and carbonic anhydrase I. The morphological and cytochemical characteristics of the cell and the constitutive expression of globin gene showed that the cells belonged to erythroid. Hemin and δ aminolevulinic acid induced the synthesis of hemoglobin, and TPA stimulated the cells to differentiate into macrophage-like cells. 该细胞系1987年由Kitamura T等建立，源于一名35岁日本男性的肝素化骨髓抽取样本，该患者具有严重的全血细胞减少症。细胞生长完全依赖于IL-3或GM-CSF，对IL-5无反应。多种淋巴因子和细胞因子对该细胞都有作用，如：IL-1、IL-4、IL-6、IL-9、IL-11、IL-13、CSF、LIF、NGF。该细胞不表达血型糖蛋白A和碳酸酐酶I。由该细胞的形态和细胞化学特征及珠蛋白基因的组成性表达，显示该细胞属于红系。氯高铁血红素和δ氨基乙酰丙酸诱导细胞合成血红蛋白，TPA刺激细胞向巨噬细胞样细胞分 RPMI 1640 (w/o Hepes) 10%FBS；4mM glutamine RPMI 1640 (w/o Hepes) 10%FBS；4mM glutamine http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C6 C6 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 78~104 78~104 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 3 � 104 ~ 5 � 105 cells/ml; and change fluid once in 2~3 days. 维持细胞浓度在3×104~5×105 cells/ml；2~3天换液1次。 NICR118 TF-1 TF-1 cell TF-1 细胞 PMID: 9271581 PMID: 9271581 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR118 cell NICR118 细胞 http://www.cellresource.cn/fdetail.aspx?id=118 RPMI 1640 (w/o Hepes) 10%FBS；4mM glutamine URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；4mM glutamine URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700139 Human Erythroleukemia Cells; TF-1 人红系白血病细胞；TF-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This cell line was established in 1987 by Kitamura T et al., and was derived from a heparinized bone marrow sample from a 35-year-old Japanese male with severe pancytopenia. Cell growth was entirely dependent on IL-3 or GM-CSF and did not respond to IL-5. Various lymphocytes and cytokines have effects on the cells, such as IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, CSF, LIF, and NGF. The cells did not express glycoprotein A and carbonic anhydrase I. The morphological and cytochemical characteristics of the cell and the constitutive expression of globin gene showed that the cells belonged to erythroid. Hemin and δ aminolevulinic acid induced the synthesis of hemoglobin, and TPA stimulated the cells to differentiate into macrophage-like cells. 该细胞系1987年由Kitamura T等建立，源于一名35岁日本男性的肝素化骨髓抽取样本，该患者具有严重的全血细胞减少症。细胞生长完全依赖于IL-3或GM-CSF，对IL-5无反应。多种淋巴因子和细胞因子对该细胞都有作用，如：IL-1、IL-4、IL-6、IL-9、IL-11、IL-13、CSF、LIF、NGF。该细胞不表达血型糖蛋白A和碳酸酐酶I。由该细胞的形态和细胞化学特征及珠蛋白基因的组成性表达，显示该细胞属于红系。氯高铁血红素和δ氨基乙酰丙酸诱导细胞合成血红蛋白，TPA刺激细胞向巨噬细胞样细胞分 RPMI 1640 (w/o Hepes) 10%FBS；8ng/ml rHUGM-csf RPMI 1640 (w/o Hepes) 10%FBS；8ng/ml rHUGM-csf http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf U U Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Maintain the cell concentration between 3 � 104 ~ 5 � 105 cells/ml; and change fluid once in 2~3 days. 维持细胞浓度在3×104~5×105 cells/ml；2~3天换液1次。 NICR707 TF-1 TF-1 cell TF-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR707 cell NICR707 细胞 http://www.cellresource.cn/fdetail.aspx?id=707 RPMI 1640 (w/o Hepes) 10%FBS；8ng/ml rHUGM-csf URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；8ng/ml rHUGM-csf URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X,Y；CSF1PO:13；D13S317:8,9；D16S539:9,12；D18S51:13；D21S11:30；D3S1358:15；D5S818:13；D7S820:12；D8S1179:11,15；FGA:18；PentaD:10,13；PentaE:5,17；TH01:7,9；TPOX:8；vWA:15,17 Amelogenin:X,Y；CSF1PO:13；D13S317:8,9；D16S539:9,12；D18S51:13；D21S11:30；D3S1358:15；D5S818:13；D7S820:12；D8S1179:11,15；FGA:18；PentaD:10,13；PentaE:5,17；TH01:7,9；TPOX:8；vWA:15,17 3111C0002000000101 Human Erythroleukemia Cells; TF-1 人红细胞白血病淋巴细胞；TF-1 lymphoblast-like 淋巴母细胞样 suspension 悬浮 The cells are IL-3 and GM-CSF dependent. 该细胞属于IL-3及GM-CSF依赖型。 10%FBS 10%FBS No generation 无代次 90%FBS+10%DMSO 90%FBS+10%DMSO Culture Method（-） 培养法（-） A类 Class A National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 2.0E + 04 cells/ml 2.0E+04cells/ml NICR2126 TF-1 TF-1 cell TF-1 细胞 NICR2126 cell NICR2126 细胞 http://www.cellresource.cn/fdetail.aspx?id=2126 Amelogenin：X；CSF1PO：9，10；D13S317：13.3；D16S539：9，10；D18S51：16，17；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：18，21；TH01：7；TPOX：8，12；vWA：16，18； Amelogenin：X；CSF1PO：9，10；D13S317：13.3；D16S539：9，10；D18S51：16，17；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：18，21；TH01：7；TPOX：8，12；vWA：16，18； 3111C0001CCC000068 Human Laryngeal Carcinoma Cells; Hep-2 人喉癌上皮细胞；Hep-2 epithelioid 上皮样 adherent growth 贴壁生长 The cell was initially thought to originate from epithelial carcinoma of the larynx, but subsequent analysis of isozyme analysis, HeLa-labeled chromosomes and DNA fingerprinting found that the cell was contaminated with HeLa; the cells were keratin-positive. 最初认为这个细胞源自喉上皮癌，但随后通过同工酶分析、HeLa标记染色体和DNA指纹分析发现，该细胞已被HeLa污染；角蛋白阳性。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 69~73 69~73 A类 Class A Hep-2-Species Identification-PUMC Hep-2-cell morphology-PUMC Hep-2-物种鉴定-PUMC Hep-2-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:10传代；每周换液2~3次。 Passage at 1: 4 ~ 1:10; and change fluid for 2~3 times a week. Hep-2 Hep-2 cell Hep-2 细胞 NICR68 PMID: 13261081 PMID: 13261081 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR68 cell NICR68 细胞 http://www.cellresource.cn/fdetail.aspx?id=68 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Hep-2-Species Identification-PUMC Hep-2-cell morphology-PUMC Hep-2-物种鉴定-PUMC Hep-2-细胞形态-PUMC Amelogenin:X；CSF1PO:9,10；D13S317:12,13.3；D16S539:9,10；D18S51:16；D21S11:27,28；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；PentaD:8,15；PentaE:7,17；TH01:7；TPOX:8,12；vWA:16,18 Amelogenin:X；CSF1PO:9,10；D13S317:12,13.3；D16S539:9,10；D18S51:16；D21S11:27,28；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；PentaD:8,15；PentaE:7,17；TH01:7；TPOX:8,12；vWA:16,18 3111C0002000000056 Human Laryngeal Carcinoma Cells; Hep-2 人喉癌上皮细胞；Hep-2 epithelioid 上皮样 adherent growth 贴壁生长 The cell was initially thought to originate from epithelial carcinoma of the larynx, but subsequent analysis of isozyme analysis, HeLa-labeled chromosomes and DNA fingerprinting found that the cell was contaminated with HeLa; the cells were keratin-positive. 最初认为这个细胞源自喉上皮癌，但随后通过同工酶分析、HeLa标记染色体和DNA指纹分析发现，该细胞已被HeLa污染；角蛋白阳性。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P370 P370 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Hep-2-cell morphology-NIFDC Hep-2-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Hep-2 Hep-2 cell Hep-2 细胞 NICR428 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation NICR428 cell NICR428 细胞 http://www.cellresource.cn/fdetail.aspx?id=428 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Hep-2-cell morphology-NIFDC Hep-2-细胞形态-NIFDC 3131C0001000700021 Human Laryngeal Carcinoma Cells; Hep-2 人喉癌上皮细胞；Hep-2 epithelial cell 上皮细胞 adherent growth 贴壁生长 The cell was initially thought to originate from epithelial carcinoma of the larynx, but subsequent analysis of isozyme analysis, HeLa-labeled chromosomes and DNA fingerprinting found that the origin cell was contaminated with HeLa. The cells were positive inimmunoperoxidase staining of keratin. 最初认为这个细胞源自喉上皮癌，但随后通过同功酶分析、HeLa标记染色体和DNA指纹分析发现，起源细胞已被HeLa污染。 角蛋白免疫过氧化物酶染色阳性。 RPMI 1640 (w/o Hepes) fetal bovine serum，20% RPMI 1640 (w/o Hepes) 胎牛血清，20% RPMI 1640 (w/o Hepes) 胎牛血清，20% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen Unit: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences Zip code: 200032 Address: No. 320, Yueyang Road, Shanghai Tel: 021-54920404，021-54920405 Fax: 021-54920531 Email: shchen@sibs.ac.cn 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920531 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 Hep-2 Hep-2 cell Hep-2 细胞 NICR592 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation of measles virus in a strain of human epidermoid cancer cells (Hep-2). Proc Soc Exp Biol Med, 1956，93: 107-108. 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation of measles virus in a strain of human epidermoid cancer cells (Hep-2). Proc Soc Exp Biol Med, 1956，93: 107-108. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR592 cell NICR592 细胞 http://www.cellresource.cn/fdetail.aspx?id=592 RPMI 1640 (w/o Hepes) 胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3142C0001000000004 Human Laryngeal Carcinoma Cells; Hep-2 人喉癌上皮细胞；Hep-2 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell was initially thought to originate from epithelial carcinoma of the larynx, but subsequent analysis of isozyme analysis, HeLa-labeled chromosomes and DNA fingerprinting found that the cell was contaminated with HeLa; the cells were keratin-positive. 最初认为这个细胞源自喉上皮癌，但随后通过同工酶分析、HeLa标记染色体和DNA指纹分析发现，该细胞已被HeLa污染；角蛋白阳性。  DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose)  10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose)  10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C360 C360 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Hep-2 Hep-2 cell Hep-2 细胞 NICR772 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR772 cell NICR772 细胞 http://www.cellresource.cn/fdetail.aspx?id=772 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose)  10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose)  10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3142C0001000000614 Human Laryngeal Carcinoma Cells; HEP-2 人喉癌细胞；HEP-2 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell is patented and its characteristics have not been disclosed. 该细胞属专利保藏，其特征特性尚未公开。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Undefined 未定 Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代，3-4天传1次 Passage at 1: 3, and passage once in 3-4 days. HEP-2 HEP-2 cell HEP-2 细胞 NICR1261 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1261 cell NICR1261 细胞 http://www.cellresource.cn/fdetail.aspx?id=1261 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001001200001 Human Synovial Sarcoma Cells; SW 982 人滑膜肉瘤细胞；SW 982 polygonal 多角 adherent growth 贴壁生长 1974年A. Leibovitz从the Scott and White Clinic, Temple, Texas 的一位25岁白人女性的纤维肉瘤手术切片中建立了SW 982细胞株。 病理组织学评价认为是与脂肪肉瘤一致的未分化恶性肿瘤。 1982年１月ATCC收到１支传代至4代的冻存管。 In 1974, A. Leibovitz established an SW 982 cell line from the fibrosarcoma surgical slices of a 25-year-old white woman from the Scott and White Clinic, Temple, Texas. Histopathological evaluation was considered to be an undifferentiated malignant tumor consistent with liposarcoma. In January 1982, ATCC received a frozen storage tube of cells passaged to generation 4. L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1582 SW 982 SW 982 cell SW 982 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1582 cell NICR1582 细胞 http://www.cellresource.cn/fdetail.aspx?id=1582 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf 3131C0001000700209 Human Synovial Sarcoma Cells; SW 982 人滑膜肉瘤细胞；SW 982 polygonal 多角 adherent growth 贴壁生长 1974年A. Leibovitz从the Scott and White Clinic, Temple, Texas 的一位25岁白人女性的纤维肉瘤手术切片中建立了SW 982细胞株。 病理组织学评价认为是与脂肪肉瘤一致的未分化恶性肿瘤。 1982年１月ATCC收到１支传代至4代的冻存管。 In 1974, A. Leibovitz established an SW 982 cell line from the fibrosarcoma surgical slices of a 25-year-old white woman from the Scott and White Clinic, Temple, Texas. Histopathological evaluation was considered to be an undifferentiated malignant tumor consistent with liposarcoma. In January 1982, ATCC received a frozen storage tube of cells passaged to generation 4. L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR2097 SW 982 SW 982 cell SW 982 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2097 cell NICR2097 细胞 http://www.cellresource.cn/fdetail.aspx?id=2097 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf Amelogenin：X，Y；CSF1PO：11，12；D13S317：8，12；D16S539：11；D18S51：13，21；D19S433：14，15.2；D21S11：31.2，33.2；D2S1338：19，23；D3S1358：15；D5S818：9；D7S820：8，12；D8S1179：13，14；FGA：20，21；TH01：6，9.3；TPOX：8，10；vWA：18； Amelogenin：X，Y；CSF1PO：11，12；D13S317：8，12；D16S539：11；D18S51：13，21；D19S433：14，15.2；D21S11：31.2，33.2；D2S1338：19，23；D3S1358：15；D5S818：9；D7S820：8，12；D8S1179：13，14；FGA：20，21；TH01：6，9.3；TPOX：8，10；vWA：18； 3111C0001CCC000075 Human Acute T Cell Leukemia Cell Line; Jurkat, Clone E6-1 人急性T淋巴细胞白血病细胞；Jurkat, Clone E6-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This cell is a clone of Jurkat-FHCRC cell line (a derivative of Jurkat cell line). Jurkat cell line is derived from the peripheral blood of a 14-year-old boy, and can produce large amounts of IL-2 (two types of inducers are needed for the production of IL-2) after induction by phorbol ester or lectin or anti-T3 monoclonal antibody, and the cells express T cell receptor, and CD3. 该细胞是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆。Jurkat细胞株来源于一个14岁男孩的外周血；经佛波酯和外源凝集素或抗T3单克隆抗体中的一种诱导后可产生大量IL-2(IL-2的产生需两种类型的诱导剂)；表达T细胞受体、CD3。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 42~46，XY 42~46，XY A类 Class A Jurkat E6-1-Cell Morphology-PUMC Jurkat E6-1-Species IdentificationPUMC Jurkat E6-1-cell morphology-PUMC Jurkat E6-1-物种鉴定PUMC Jurkat E6-1-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration in 1 � 105 ~ 1 � 106viable cells/ml; and change fluid once in 2 to 3 days. 维持细胞浓度在1×105～1×106viable cells/ml；2～3天换液1次。　 Jurkat, Clone E6-1 Jurkat, Clone E6-1 cell Jurkat, Clone E6-1 细胞 NICR75 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR75 cell NICR75 细胞 http://www.cellresource.cn/fdetail.aspx?id=75 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Jurkat E6-1-Cell Morphology-PUMC Jurkat E6-1-Species IdentificationPUMC Jurkat E6-1-cell morphology-PUMC Jurkat E6-1-物种鉴定PUMC Jurkat E6-1-细胞形态-PUMC Jurkat E6-1-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,12；D16S539:11；D18S51:13,21；D21S11:31.2,33.2；D3S1358:15；D5S818:9；D7S820:8,12；D8S1179:13,14；FGA:20,21；PentaD:11,13；PentaE:10,12；TH01:6,9.3；TPOX:8,10；vWA:18 Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,12；D16S539:11；D18S51:13,21；D21S11:31.2,33.2；D3S1358:15；D5S818:9；D7S820:8,12；D8S1179:13,14；FGA:20,21；PentaD:11,13；PentaE:10,12；TH01:6,9.3；TPOX:8,10；vWA:18 3111C0002000000049 Human Acute T Cell Leukemia Cell Line; Jurkat, Clone E6-1 人急性T淋巴细胞白血病细胞；Jurkat, Clone E6-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This cell is a clone of Jurkat-FHCRC cell line (a derivative of Jurkat cell line). Jurkat cells are derived from the peripheral blood of a 14-year-old boy. A large amount of IL-2 (two kinds of inducers are needed for IL-2 production) can be produced after induction by phorbol ester and lectin or anti-T3 monoclonal antibody, and the cells express T cell receptor, and CD3. 该细胞是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆。 Jurkat细胞株来源于一个14岁男孩的外周血。 经佛波酯和外源凝集素或抗T3单克隆抗体诱导后可产生大量IL-2(IL-2的产生需两种类型的诱导剂)；表达T细胞受体、CD3. RPMI 1640 (w/o Hepes) fetal bovine serum 10% RPMI 1640 (w/o Hepes) 胎牛血清 10% RPMI 1640 (w/o Hepes) 胎牛血清 10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A jurkat clone E6-1-cell morphology-NIFDC jurkat clone E6-1-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1x10-5 cells/ml 1x10-5细胞/ml Jurkat, Clone E6-1 Jurkat, Clone E6-1 cell Jurkat, Clone E6-1 细胞 NICR421 NICR421 cell NICR421 细胞 http://www.cellresource.cn/fdetail.aspx?id=421 RPMI 1640 (w/o Hepes) 胎牛血清 10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清 10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf jurkat clone E6-1-cell morphology-NIFDC jurkat clone E6-1-细胞形态-NIFDC 3131C0001000700123 Human Acute T Cell Leukemia Cell Line; Jurkat, Clone E6-1 人急性T淋巴细胞白血病细胞；Jurkat, Clone E6-1 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 This cell is a clone of Jurkat-FHCRC cell line (a derivative of Jurkat cell line). Jurkat cells are derived from the peripheral blood of a 14-year-old boy. Large amounts of IL-2 can be produced after induction by phorbol ester and lectin or anti-T3 monoclonal antibody (both types of induction are required). The cells are mycoplasma-negative. 这是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆。 Jurkat细胞株来源于一个14岁男孩的外周血。 经佛波酯和外源凝集素或抗T3单克隆抗体(两种类型的诱导都需要)诱导后可产生大量IL-2。 支原体阴性。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Jurkat clone E6-1-cell morphology-SHCRC Jurkat clone E6-1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. Jurkat, Clone E6-1 Jurkat, Clone E6-1 cell Jurkat, Clone E6-1 细胞 NICR691 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR691 cell NICR691 细胞 http://www.cellresource.cn/fdetail.aspx?id=691 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Jurkat clone E6-1-cell morphology-SHCRC Jurkat clone E6-1-细胞形态-SHCRC Amelogenin：X，Y；CSF1PO：11，12；D13S317：8，12；D16S539：11；D18S51：13，21；D19S433：14，15.2；D21S11：31.2，33.2；D2S1338：19，23；D3S1358：15；D5S818：9；D7S820：8，12；D8S1179：13，14；FGA：20，21；TH01：6，9.3；TPOX：8，10；vWA：18； Amelogenin：X，Y；CSF1PO：11，12；D13S317：8，12；D16S539：11；D18S51：13，21；D19S433：14，15.2；D21S11：31.2，33.2；D2S1338：19，23；D3S1358：15；D5S818：9；D7S820：8，12；D8S1179：13，14；FGA：20，21；TH01：6，9.3；TPOX：8，10；vWA：18； 3111C0001CCC000348 Human Acute T Cell Leukemia Cell Line; JurkatE6-1 人急性T淋巴细胞白血病细胞；JurkatE6-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This cell is a clone of Jurkat-FHCRC cells, a derivative of Jurkat cells. The cells can produce large amounts of IL-2 after stimulation with phorbol ester or exogenous lectin or T3 monoclonal antibody. 该细胞是Jurkat-FHCRC细胞的克隆，是Jurkat细胞的衍生物。佛波酯或外源凝集素或T3单抗刺激后该细胞可产生大量的IL-2。 RPMI 1640 (w/o Hepes) 10% FBS RPMI 1640 (w/o Hepes) 10% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C15 C15 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 46,XY 46,XY A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Keep the cell density between 1 � 105 ~ 1 � 106 cells/ml, no more than 3 � 106 cells/ml. 保持细胞密度在1×105～1×106 cells/ml之间，不超过3×106 cells/ml。 JurkatE6-1 JurkatE6-1 cell JurkatE6-1 细胞 NICR1537 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1537 cell NICR1537 细胞 http://www.cellresource.cn/fdetail.aspx?id=1537 RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：11，12；D13S317：8，11；D16S539：11；D18S51：13，21；D19S433：13，15.2；D21S11：31.2，33.2；D2S1338：19，23；D3S1358：15，17；D5S818：9；D7S820：8，10；D8S1179：12，14；FGA：19，22；TH01：6，9.3；TPOX：8，10；vWA：17，18； Amelogenin：X；CSF1PO：11，12；D13S317：8，11；D16S539：11；D18S51：13，21；D19S433：13，15.2；D21S11：31.2，33.2；D2S1338：19，23；D3S1358：15，17；D5S818：9；D7S820：8，10；D8S1179：12，14；FGA：19，22；TH01：6，9.3；TPOX：8，10；vWA：17，18； 3111C0001CCC000241 Human Acute T-lymphocytic Leukemia Cells; I 2.1(CRL-2572) 人急性T淋巴细胞性白血病细胞；I 2.1(CRL-2572) lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 This cell is a FADD mutant of wild-type Jurkat cell line A3, which does not express FADD protein and is resistant to Fas-mediated death. 该细胞为野生型Jurkat细胞系A3的FADD突变体；不表达FADD蛋白，抵抗Fas介导的死亡。 RPMI 1640 (w/o Hepes) 15%FBS；1mM Hepes＋1mM sodium pyruvate RPMI 1640 (w/o Hepes) 15%FBS；1mM Hepes＋1mM sodium pyruvate http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A I2-1-Species Identification-PUMC I2-1-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 0.2 ~ 2 � 106 cells/ml; and change fluid once in 2 to 3 days. 维持细胞浓度在0.2～2×106 cells/ml之间；2～3天换液1次。 I 2.1(CRL-2572) I 2.1(CRL-2572) cell I 2.1(CRL-2572) 细胞 NICR241 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR241 cell NICR241 细胞 http://www.cellresource.cn/fdetail.aspx?id=241 RPMI 1640 (w/o Hepes) 15%FBS；1mM Hepes＋1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 15%FBS；1mM Hepes＋1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108101040.pdf I2-1-Species Identification-PUMC I2-1-物种鉴定-PUMC 3131C0001000700207 Human Acute Monocytic Leukemia Mononuclear Cells; AML-193 人急性单核细胞白血病单核细胞；AML-193 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 Interleukin-3 (IL-3) and granulocyte macrophage colony-stimulating factor (GM-CSF) synergistically stimulate cell growth. Granulocyte colony-stimulating factor (G-CSF) also supports short-term and long-term growth of AML-193 cells and induces cell proliferation together with GM-CSF. This cell line was formally named as ATCC HTB-188. 白细胞介素3(IL-3)和粒细胞巨噬细胞集落刺激因子(GM-CSF)协同刺激细胞生长。 粒细胞集落刺激因子(G-CSF)也AML-193细胞支持短期和长期生长，并与GM-CSF一起诱导细胞增殖。 这株细胞正式命名为ATCC HTB-188。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，5%；0.005 mg/ml insulin, 0.005 mg/ml transferrin and 5 ng/ml GM-CSF IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，5%；0.005 mg/ml insulin, 0.005 mg/ml transferrin 和 5 ng/ml GM-CSF IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，5%；0.005 mg/ml insulin, 0.005 mg/ml transferrin 和 5 ng/ml GM-CSF http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. AML-193 AML-193 cell AML-193 细胞 NICR1712 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1712 cell NICR1712 细胞 http://www.cellresource.cn/fdetail.aspx?id=1712 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，5%；0.005 mg/ml insulin, 0.005 mg/ml transferrin 和 5 ng/ml GM-CSF URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，5%；0.005 mg/ml insulin, 0.005 mg/ml transferrin 和 5 ng/ml GM-CSF URI: http://www.cellresource.cn/pdf/20080108100822.pdf Amelogenin：X，Y；CSF1PO：11，13；D13S317：8，13；D16S539：11，12；D18S51：13，14，15；D19S433：12.2，13，14；D21S11：29，30，31.2；D2S1338：17，18；D3S1358：15，16，17；D5S818：11，12；D7S820：10；D8S1179：10，14；FGA：22，24，25；TH01：7，8，9.3；TPOX：8，11；vWA：16； Amelogenin：X，Y；CSF1PO：11，13；D13S317：8，13；D16S539：11，12；D18S51：13，14，15；D19S433：12.2，13，14；D21S11：29，30，31.2；D2S1338：17，18；D3S1358：15，16，17；D5S818：11，12；D7S820：10；D8S1179：10，14；FGA：22，24，25；TH01：7，8，9.3；TPOX：8，11；vWA：16； 3111C0001CCC000057 Human Acute Monocytic Leukemia Cells; THP-1 人急性单核细胞白血病细胞；THP-1 monocyte 单核细胞 suspension growth 悬浮生长 The cells were isolated and established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia. The cells can phagocytize latex particles and activated erythrocytes, with no immunoglobulin in both cell membrane and cytoplasm. and express C3R and FcR; The cells can be induced to differentiate into mononuclear cells by phorbol ester TPA and can be used as transfection host. 该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细胞可以吞噬乳胶颗粒和激活的红细胞，细胞膜和胞浆内均没有免疫球蛋白，表达C3R和FcR；可受佛波酯TPA诱导向单核系方向分化；可作为转染宿主。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 80~84，48~52 80~84，48~52 A类 Class A THP-1-Species Identification-PUMC THP-1-cell morphology-PUMC THP-1-物种鉴定-PUMC THP-1-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 2 ~ 4 � 105 ~ 8 � 105/ml, do not exceed 1 � 106/ml; and change fluid once in 2~3 days. 维持细胞浓度在2~4×105~8×105/ml，勿超过1×106/ml；2~3天换液1次。　 NICR57 THP-1 THP-1 cell THP-1 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR57 cell NICR57 细胞 http://www.cellresource.cn/fdetail.aspx?id=57 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf THP-1-Species Identification-PUMC THP-1-cell morphology-PUMC THP-1-物种鉴定-PUMC THP-1-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:11,13；D13S317:13；D16S539:11,12；D18S51:13,14；D21S11:30,31.2；D3S1358:15,17；D5S818:11,12；D7S820:10；D8S1179:10,14；FGA:24,25；PentaD:10,12；PentaE:11,15；TH01:8,9.3；TPOX:8,11；vWA:16 Amelogenin:X,Y；CSF1PO:11,13；D13S317:13；D16S539:11,12；D18S51:13,14；D21S11:30,31.2；D3S1358:15,17；D5S818:11,12；D7S820:10；D8S1179:10,14；FGA:24,25；PentaD:10,12；PentaE:11,15；TH01:8,9.3；TPOX:8,11；vWA:16 3111C0002000000058 Human Acute Monocytic Leukemia Cells; THP-1 人急性单核细胞白血病细胞；THP-1 monocyte 单核细胞 suspension growth 悬浮生长 The cells were isolated and established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia. The cells can phagocytize latex particles and activated erythrocytes, with no immunoglobulin in both cell membrane and cytoplasm. and express C3R and FcR; The cells can be induced to differentiate into mononuclear cells by phorbol ester TPA and can be used as transfection host. 该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细胞可以吞噬乳胶颗粒和激活的红细胞，细胞膜和胞浆内均没有免疫球蛋白，表达C3R和FcR；可受佛波酯TPA诱导向单核系方向分化；可作为转染宿主。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A THP-1-cell morphology-NIFDC THP-1-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net Maintain the cell concentration between 2 ~ 4 � 105 ~ 8 � 105/ml, do not exceed 1 � 106/ml; and change fluid once in 2~3 days. 维持细胞浓度在2~4×105~8×105/ml，勿超过1×106/ml；2~3天换液1次。　 NICR430 THP-1 THP-1 cell THP-1 细胞 NICR430 cell NICR430 细胞 http://www.cellresource.cn/fdetail.aspx?id=430 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf THP-1-cell morphology-NIFDC THP-1-细胞形态-NIFDC 3131C0001000700057 Human Acute Monocytic Leukemia Cells; THP-1 人急性单核细胞白血病细胞；THP-1 monocyte 单核细胞 suspension growth 悬浮生长 The cells have phagocytosis to milk beads and activated erythrocytes, and have no surface and cytoplasmic immunoglobulin. Monocyte differentiation can be induced with phorbol ester TPA. 该细胞对乳汁珠和激活的红细胞有吞噬作用，无表面和胞质免疫球蛋白。 可以用佛波醇 TPA诱导单核细胞分化。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10%；0.05mM β-mercaptoethanol RPMI 1640 (w/o Hepes) 优质胎牛血清，10%；0.05mM β-巯基乙醇 RPMI 1640 (w/o Hepes) 优质胎牛血清，10%；0.05mM β-巯基乙醇 http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NICR625 THP-1 THP-1 cell THP-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR625 cell NICR625 细胞 http://www.cellresource.cn/fdetail.aspx?id=625 RPMI 1640 (w/o Hepes) 优质胎牛血清，10%；0.05mM β-巯基乙醇 URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10%；0.05mM β-巯基乙醇 URI: http://www.cellresource.cn/pdf/20080108101040.pdf STR Identification STR鉴定 3142C0001000000114 Human Acute Monocytic Leukemia Cells; THP-1 人急性单核细胞白血病细胞；THP-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells were isolated and established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia. The cells can phagocytize latex particles and activated erythrocytes, with no immunoglobulin in both cell membrane and cytoplasm. and express C3R and FcR; The cells can be induced to differentiate into mononuclear cells by phorbol ester TPA and can be used as transfection host. 该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细胞可以吞噬乳胶颗粒和激活的红细胞，细胞膜和胞浆内均没有免疫球蛋白，表达C3R和FcR；可受佛波酯TPA诱导向单核系方向分化；可作为转染宿主。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C54 C54 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR882 THP-1 THP-1 cell THP-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR882 cell NICR882 细胞 http://www.cellresource.cn/fdetail.aspx?id=882 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700131 Human Acute Non-B, Non-T Lymphocytic Leukemia Cells; Reh 人急性非B非T淋巴细胞性白血病细胞；Reh lymphoblast 淋巴母细胞 suspension growth 悬浮生长 CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%) CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%) RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Reh-DNA-Fingerprinting-SHCRC Reh-DNA-指纹-SHCRC Reh-cell morphology-SHCRC Reh-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. NICR699 Reh Reh cell Reh 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR699 cell NICR699 细胞 http://www.cellresource.cn/fdetail.aspx?id=699 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Reh-DNA-Fingerprinting-SHCRC Reh-DNA-指纹-SHCRC Reh-cell morphology-SHCRC Reh-细胞形态-SHCRC 3142C0001000000322 Human Acute Lymphoblastic Leukemia Cells; Kasumi-1 人急性淋巴白血病细胞；Kasumi-1 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells were exchanged and preserved by Professor Gong Feili, Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology. Kasumi-1 cells has the typical characteristics of human acute lymphoblastic leukemia cells and are excellent materials for the study of human acute lymphoblastic leukemia. 该细胞由华中科技大学同济医学院免疫系龚非力教授交换保藏。Kasumi-1细胞且有人急性淋巴白血病细胞的典型特征，是研究人急性淋巴白血病的极佳材料。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Kasumi-1 Kasumi-1 cell Kasumi-1 细胞 NICR1973 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1973 cell NICR1973 细胞 http://www.cellresource.cn/fdetail.aspx?id=1973 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X,Y；CSF1PO:11,12,13；D13S317:12,13；D16S539:11,14；D18S51:13,17；D19S433:14,15；D21S11:28,29,30,31；D2S1338:23,24；D3S1358:14,16；D5S818:12；D7S820:8,10,11；D8S1179:9,14；FGA:22,23,25；TH01:6,8；TPOX:8；vWA:17,18 Amelogenin:X,Y；CSF1PO:11,12,13；D13S317:12,13；D16S539:11,14；D18S51:13,17；D19S433:14,15；D21S11:28,29,30,31；D2S1338:23,24；D3S1358:14,16；D5S818:12；D7S820:8,10,11；D8S1179:9,14；FGA:22,23,25；TH01:6,8；TPOX:8；vWA:17,18 3131C0001001200018 Human Acute Lymphoblastic Leukemia Cells; Molt-4 人急性淋巴母细胞白血病细胞；Molt-4 lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 MOLT-4 and MOLT-3 are derived from a 19-year-old male patient with relapse of acute lymphoblastic leukemia who received multi-drug combination chemotherapy. The MOLT-4 cell line is of T lymphocyte origin and has a G → A mutation at codon 248 of p53 gene, and does not express p53, immunoglobulin or Epstein-Barr virus; it can produce a high level of terminal deoxyribonucleotidyl-transferase; it can express CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%). MOLT-4与MOLT-3来源于一名19岁的男性急性淋巴细胞性白血病的复发患者，该患者前期接受过多种药物联合化疗。MOLT-4细胞系为T淋巴细胞起源，p53基因的第248位密码子有一个G→A突变，不表达p53，不表达免疫球蛋白或EB病毒；可产生高水平的末端脱氧核糖转移酶；表达CD1 (49%), CD2 (35%), CD3 A (26%) B(33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B MOLT-4-DNA-Fingerprinting-SHCRC MOLT-4-DNA-指纹-SHCRC MOLT-4-cell morphology-SHCRC MOLT-4-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Maintain the cell concentration between 4 � 105/ml ~ 2 � 106/ml; and replenish the fluid according to the cell concentration every 2 to 3 days. 维持细胞浓度在4×105/ml~2×106/ml；根据细胞浓度每2~3天补液1次。 Molt-4 Molt-4 cell Molt-4 细胞 NICR1599 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1599 cell NICR1599 细胞 http://www.cellresource.cn/fdetail.aspx?id=1599 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf MOLT-4-DNA-Fingerprinting-SHCRC MOLT-4-DNA-指纹-SHCRC MOLT-4-cell morphology-SHCRC MOLT-4-细胞形态-SHCRC Amelogenin:X,Y；CSF1PO:11,12,13；D13S317:12,13；D16S539:11,14；D18S51:13,17；D19S433:14,15；D21S11:28,29,30,31；D2S1338:23,24；D3S1358:14,16；D5S818:12；D7S820:8,10,11；D8S1179:9,14；FGA:22,23,25；TH01:6,8；TPOX:8；vWA:17,18 Amelogenin:X,Y；CSF1PO:11,12,13；D13S317:12,13；D16S539:11,14；D18S51:13,17；D19S433:14,15；D21S11:28,29,30,31；D2S1338:23,24；D3S1358:14,16；D5S818:12；D7S820:8,10,11；D8S1179:9,14；FGA:22,23,25；TH01:6,8；TPOX:8；vWA:17,18 3111C0001CCC000043 Human Acute Lymphoblastic Leukemia Cells; MOLT-4 人急性淋巴母细胞性白血病细胞；MOLT-4 lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 MOLT-4 and MOLT-3 are derived from a 19-year-old male patient with relapse of acute lymphoblastic leukemia who received multi-drug combination chemotherapy. The MOLT-4 cell line is of T lymphocyte origin and has a G → A mutation at codon 248 of p53 gene, and does not express p53, immunoglobulin or Epstein-Barr virus; it can produce a high level of terminal deoxyribonucleotidyl-transferase; it can express CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%). MOLT-4与MOLT-3来源于一名19岁的男性急性淋巴细胞性白血病的复发患者，该患者前期接受过多种药物联合化疗。MOLT-4细胞系为T淋巴细胞起源，p53基因的第248位密码子有一个G→A突变，不表达p53，不表达免疫球蛋白或EB病毒；可产生高水平的末端脱氧核糖转移酶；表达CD1 (49%), CD2 (35%), CD3 A (26%) B(33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 84~89 84~89 A类 Class A Molt-4-Species Identification-PUMC Molt-4-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 4 � 105/ml ~ 2 � 106/ml; and replenish the fluid according to the cell concentration every 2 to 3 days. 维持细胞浓度在4×105/ml~2×106/ml；根据细胞浓度每2~3天补液1次。 MOLT-4 MOLT-4 cell MOLT-4 细胞 NICR43 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR43 cell NICR43 细胞 http://www.cellresource.cn/fdetail.aspx?id=43 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Molt-4-Species Identification-PUMC Molt-4-物种鉴定-PUMC Amelogenin:X,Y；CSF1PO:11,12,13；D13S317:12,13；D16S539:11,14；D18S51:13,17；D19S433:14,15；D21S11:28,29,30,31；D2S1338:23,24；D3S1358:14,16；D5S818:12；D7S820:8,10,11；D8S1179:9,14；FGA:22,23,25；TH01:6,8；TPOX:8；vWA:17,18 Amelogenin:X,Y；CSF1PO:11,12,13；D13S317:12,13；D16S539:11,14；D18S51:13,17；D19S433:14,15；D21S11:28,29,30,31；D2S1338:23,24；D3S1358:14,16；D5S818:12；D7S820:8,10,11；D8S1179:9,14；FGA:22,23,25；TH01:6,8；TPOX:8；vWA:17,18 3131C0001000700037 Human Acute Lymphoblastic Leukemia Cells; MOLT-4 人急性淋巴母细胞性白血病细胞；MOLT-4 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 This cell line was established from the cells of a recurrent patient. The patient had received a multi-drug combination pre-chemotherapy. There was a G- > A mutation in codon 248 of p53 gene. The P53 protein was not expressed. No immunoglobulin or Epstein-Barr virus was produced. MOLT-4 and MOLT-3 were derived from the same patient. The cells was once contaminated with mycoplasma but was removed with antibiotics. CD3 expression is lost. 这个细胞株从一位复发病人的细胞中建立。 这个病人接受过多种药物联合前期化疗。 p53基因的248位密码子有一个G -> A突变。 P53不表达。 不生成免疫球蛋白或EB病毒。 MOLT-4与MOLT-3来源于同一位病人。 曾受支原体污染，但用抗生素去除。CD3丢失 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A MOLT-4-DNA-Fingerprinting-SHCRC MOLT-4-DNA-指纹-SHCRC MOLT-4-cell morphology-SHCRC MOLT-4-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. MOLT-4 MOLT-4 cell MOLT-4 细胞 NICR607 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR607 cell NICR607 细胞 http://www.cellresource.cn/fdetail.aspx?id=607 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf MOLT-4-DNA-Fingerprinting-SHCRC MOLT-4-DNA-指纹-SHCRC MOLT-4-cell morphology-SHCRC MOLT-4-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000079 Human Acute Lymphoblastic Leukemia Cells; MOLT-4 人急性淋巴母细胞性白血病细胞；MOLT-4 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 MOLT-4 and MOLT-3 are derived from a 19-year-old male patient with relapse of acute lymphoblastic leukemia who received multi-drug combination chemotherapy. The MOLT-4 cell line is of T lymphocyte origin and has a G → A mutation at codon 248 of p53 gene, and does not express p53, immunoglobulin or Epstein-Barr virus; it can produce a high level of terminal deoxyribonucleotidyl-transferase; it can express CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), D6 (22%), CD7 (77%). MOLT-4与MOLT-3来源于一名19岁的男性急性淋巴细胞性白血病的复发患者，该患者前期接受过多种药物联合化疗。MOLT-4细胞系为T淋巴细胞起源，p53基因的第248位密码子有一个G→A突变，不表达p53，不表达免疫球蛋白或EB病毒；可产生高水平的末端脱氧核糖转移酶；表达CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C12 C12 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. MOLT-4 MOLT-4 cell MOLT-4 细胞 NICR847 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR847 cell NICR847 细胞 http://www.cellresource.cn/fdetail.aspx?id=847 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X,Y；CSF1PO:11,12,13；D13S317:12,13；D16S539:11,14；D18S51:13,17；D19S433:14,15；D21S11:28,29,30,31；D2S1338:23,24；D3S1358:14,16；D5S818:12；D7S820:8,10,11；D8S1179:9,14；FGA:22,23,25；TH01:6,8；TPOX:8；vWA:17,18 Amelogenin:X,Y；CSF1PO:11,12,13；D13S317:12,13；D16S539:11,14；D18S51:13,17；D19S433:14,15；D21S11:28,29,30,31；D2S1338:23,24；D3S1358:14,16；D5S818:12；D7S820:8,10,11；D8S1179:9,14；FGA:22,23,25；TH01:6,8；TPOX:8；vWA:17,18 3131C0001000700226 Human Acute Lymphoblastic Leukemia Cells; MOLT-4 人急性淋巴母细胞性白血病细胞；MOLT-4 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 This cell line was established from the cells of a recurrent patient. The patient had received a multi-drug combination pre-chemotherapy. There was a G- > A mutation in codon 248 of p53 gene. The P53 protein was not expressed. No immunoglobulin or Epstein-Barr virus was produced. MOLT-4 and MOLT-3 were derived from the same patient. The cells was once contaminated with mycoplasma but was removed with antibiotics. 这个细胞株从一位复发病人的细胞中建立。 这个病人接受过多种药物联合前期化疗。 p53基因的248位密码子有一个G -> A突变。 P53不表达。 不生成免疫球蛋白或EB病毒。 MOLT-4与MOLT-3来源于同一位病人。 曾受支原体污染，但用抗生素去除。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A MOLT-4-DNA-Fingerprinting-SHCRC MOLT-4-DNA-指纹-SHCRC MOLT-4-cell morphology-SHCRC MOLT-4-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. MOLT-4 MOLT-4 cell MOLT-4 细胞 NICR2105 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2105 cell NICR2105 细胞 http://www.cellresource.cn/fdetail.aspx?id=2105 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf MOLT-4-DNA-Fingerprinting-SHCRC MOLT-4-DNA-指纹-SHCRC MOLT-4-cell morphology-SHCRC MOLT-4-细胞形态-SHCRC 3131C0001000700129 Human Acute Lymphoblastic Leukemia T-Lymphocytes; CCRF-CEM [CCRF CEM] 人急性淋巴细胞白血病T淋巴细胞；CCRF-CEM [CCRF CEM] lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 G.E. Foley et al established the lymphoblast-like cell line CCRF-CEM. The cells were obtained in November 1964 from peripheral blood leukocytes in a four -year-old white female patient with acute lymphoblastic leukemia. The cell line was collected from Hong Kong. G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。此细胞系从香港收集而来。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. CCRF CEM CCRF CEM cell CCRF CEM 细胞 CCRF-CEM CCRF-CEM cell CCRF-CEM 细胞 NICR697 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR697 cell NICR697 细胞 http://www.cellresource.cn/fdetail.aspx?id=697 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700147 Human Acute Lymphoblastic Leukemia T-Lymphocytes; CCRF-CEM [CCRF CEM] 人急性淋巴细胞白血病T淋巴细胞；CCRF-CEM [CCRF CEM] lymphoblast 淋巴母细胞 suspension growth 悬浮生长 G.E. Foley et al established the lymphoblast-like cell line CCRF-CEM. The cells were obtained in November 1964 from peripheral blood leukocytes in a four -year-old white female patient with acute lymphoblastic leukemia. G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. CCRF CEM CCRF CEM cell CCRF CEM 细胞 CCRF-CEM CCRF-CEM cell CCRF-CEM 细胞 NICR713 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR713 cell NICR713 细胞 http://www.cellresource.cn/fdetail.aspx?id=713 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001001200011 Human Acute Lymphoblastic Leukemia Cells; CEM/C1 人急性淋巴细胞白血病细胞；CEM/C1 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 CEM/C1 is a derivative of human T cell leukemia cell line CCRF-CEM (see ATCC CCL-119) with camptothecin resistance. In 1991, the cell lines were selected and subcloned with the resistance to CPT. The cells show cross resistance to water-soluble topotecan and water-insoluble 9-aminocamptothecin and cross-resistance to 10,11-methylenedioxycamptothecin. The sensitivity of CEM/C1 cells to CPT was 31 times lower than that of CEM cells. CEM/C1 cells show atypical multi-drug resistance and catalytic activity of transforming the topoisomerase I. The resistance to CPT can maintain for more than 6 months. CEM/C1是人T细胞白血病细胞株CCRF-CEM(见ATCC CCL-119)具有喜树碱抗性的衍生株。 1991年细胞株选择并亚克隆了对CPT的抗性。 细胞表现出对CPT类似物水溶性的托泊替康和非水溶性的9-氨基-CPT及10,11-亚甲二氧基-CPT具有交叉抗性。 CEM/C1细胞对CPT的敏感性较母系CEM细胞低31倍。 CEM/C1细胞表现非典型的多药抗性和转换拓补异构酶I催化活性。 对CPT的抗性维持6个月以上。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A CEM-C1-cell morpholgoy-SHCRC CEM-C1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. CEM/C1 CEM/C1 cell CEM/C1 细胞 NICR1592 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1592 cell NICR1592 细胞 http://www.cellresource.cn/fdetail.aspx?id=1592 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf CEM-C1-cell morpholgoy-SHCRC CEM-C1-细胞形态-SHCRC 3131C0001000700212 Human Acute Lymphoblastic Leukemia Cells; CEM/C1 人急性淋巴细胞白血病细胞；CEM/C1 lymphoblast 淋巴母细胞 suspension growth 悬浮生长 CEM/C1 is a derivative of human T cell leukemia cell line CCRF-CEM (see ATCC CCL-119) with camptothecin resistance. In 1991, the cell lines were selected and subcloned with the resistance to CPT. The cells show cross resistance to water-soluble topotecan and water-insoluble 9-aminocamptothecin and cross-resistance to 10,11-methylenedioxycamptothecin. The sensitivity of CEM/C1 cells to CPT was 31 times lower than that of CEM cells. CEM/C1 cells show atypical multi-drug resistance and catalytic activity of transforming the topoisomerase I. The resistance to CPT can maintain for more than 6 months. CEM/C1是人T细胞白血病细胞株CCRF-CEM(见ATCC CCL-119)具有喜树碱抗性的衍生株。 1991年细胞株选择并亚克隆了对CPT的抗性。 细胞表现出对CPT类似物水溶性的托泊替康和非水溶性的9-氨基-CPT及10,11-亚甲二氧基-CPT具有交叉抗性。 CEM/C1细胞对CPT的敏感性较母系CEM细胞低31倍。 CEM/C1细胞表现非典型的多药抗性和转换拓补异构酶I催化活性。 对CPT的抗性维持6个月以上。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A CEM-C1-cell morpholgoy-SHCRC CEM-C1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. CEM/C1 CEM/C1 cell CEM/C1 细胞 NICR1716 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1716 cell NICR1716 细胞 http://www.cellresource.cn/fdetail.aspx?id=1716 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf CEM-C1-cell morpholgoy-SHCRC CEM-C1-细胞形态-SHCRC Amelogenin:X,Y；CSF1PO:7；D13S317:11,12；D16S539:10,11；D18S51:10.2,18；D21S11:28,29；D3S1358:15,16；D5S818:13；D7S820:8,10；D8S1179:13,14；FGA:22；PentaD:8,9；PentaE:7,13；TH01:7,8；TPOX:7,9；vWA:14,19 Amelogenin:X,Y；CSF1PO:7；D13S317:11,12；D16S539:10,11；D18S51:10.2,18；D21S11:28,29；D3S1358:15,16；D5S818:13；D7S820:8,10；D8S1179:13,14；FGA:22；PentaD:8,9；PentaE:7,13；TH01:7,8；TPOX:7,9；vWA:14,19 3111C0002000000028 Human Acute Myeloid Leukemia Cells; KG-1a 人急性髓性白血病细胞；KG-1a myeloma cell-like 骨髓瘤细胞样 adherent growth 贴壁生长 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium new-born calf serum，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 新生牛血清，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 新生牛血清，20% http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A KG-1a-cell morphology-NIFDC KG-1a-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 2x10-5 cells/ml 2x10-5细胞/ml KG-1a KG-1a cell KG-1a 细胞 NICR400 NICR400 cell NICR400 细胞 http://www.cellresource.cn/fdetail.aspx?id=400 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 新生牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 新生牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf KG-1a-cell morphology-NIFDC KG-1a-细胞形态-NIFDC Amelogenin：X；CSF1PO：13，14；D13S317：8，11；D16S539：11；D18S51：14，15；D19S433：14；D21S11：29，30；D2S1338：17；D3S1358：16；D5S818：12；D7S820：11，12；D8S1179：13；FGA：22，24；TH01：7，8；TPOX：8，11；vWA：16； Amelogenin：X；CSF1PO：13，14；D13S317：8，11；D16S539：11；D18S51：14，15；D19S433：14；D21S11：29，30；D2S1338：17；D3S1358：16；D5S818：12；D7S820：11，12；D8S1179：13；FGA：22，24；TH01：7，8；TPOX：8，11；vWA：16； 3111C0001CCC000037 Human Acute Promyelocytic Leukemia Cells; HL-60 人急性早幼粒白血病细胞；HL-60 medullary cell-like 髓母细胞样 suspension growth 悬浮生长 The cells were isolated from the peripheral blood of a 36-year-old white woman with acute promyelocytic leukemia by Collins SJ; they can spontaneously differentiate, or differentiate under the stimulation of butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), DMSO(1% to 1.5%), actinomycin D and retinoic acid. TNF-α can be secreted after simulation by PMA. This cell has phagocytosis activity and chemotaxis reaction, oncogene myc-positive, expressing complement receptor and FcR. 该细胞由Collins SJ从一位患有急性早幼粒细胞性白血病的36岁白人女性的外周血中分离建立；可自发分化，或在丁酸盐、次黄嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1% to 1.5%)、放线菌素D和视黄酸的刺激下发生分化；PMA刺激后可分泌TNF-α。该细胞具有吞噬活性和趋化反应，癌基因myc阳性，表达补体受体和FcR。 RPMI 1640 (w/o Hepes) 20%FBS RPMI 1640 (w/o Hepes) 20%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 34~43 34~43 A类 Class A HL-60-cell morphology-PUMC HL-60-细胞形态-PUMC HL60-Cell Morphology-PUMC HL60-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 1 � 105 ~ 1 � 106/ml, and change fluid once every 2~3 day. 维持细胞浓度在1×105~1×106/ml,每2~3天换液1次。 HL-60 HL-60 cell HL-60 细胞 NICR37 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR37 cell NICR37 细胞 http://www.cellresource.cn/fdetail.aspx?id=37 RPMI 1640 (w/o Hepes) 20%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 20%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf HL-60-cell morphology-PUMC HL-60-细胞形态-PUMC HL60-Cell Morphology-PUMC HL60-细胞形态-PUMC Amelogenin:X；CSF1PO:13,14；D13S317:8,11；D16S539:11；D18S51:14,15；D19S433:14；D21S11:29,30；D2S1338:17；D3S1358:16；D5S818:12；D7S820:11,12；D8S1179:12,13；FGA:22,24；TH01:7,8；TPOX:8,11；vWA:16 Amelogenin:X；CSF1PO:13,14；D13S317:8,11；D16S539:11；D18S51:14,15；D19S433:14；D21S11:29,30；D2S1338:17；D3S1358:16；D5S818:12；D7S820:11,12；D8S1179:12,13；FGA:22,24；TH01:7,8；TPOX:8,11；vWA:16 3131C0001000700023 Human Acute Promyelocytic Leukemia Cells; HL-60 人急性早幼粒白血病细胞；HL-60 myeloblast 原粒细胞 suspension growth 悬浮生长 HL-60 is a promyelocytic cell line. Peripheral blood leukocytes come from a 36-year-old white woman with acute granular-monocytic leukemia. HL-60 can spontaneously differentiate, and butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), DMSO (1% to 1.5%), actinomycin D and retinoic acid can promote differentiation. The cells show phagocytosis activity and respond to chemotaxis stimulation. The oncogenic gene myc expression is positive. HL-60是一株早幼粒细胞。 外周血白细胞来自一位患有急性粒-单核细胞白血病的36岁白人女性。 HL-60 自发分化，丁酸盐、次黄嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1% to 1.5%)、放线菌素D和视黄酸可以促进分化。 细胞表现出吞噬活性，并对趋化刺激有响应。致癌基因myc表达阳性。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，20% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，20% http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A HL60-cell morphology-SHCRC HL60-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. HL-60 HL-60 cell HL-60 细胞 NICR594 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR594 cell NICR594 细胞 http://www.cellresource.cn/fdetail.aspx?id=594 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，20% URI: http://www.cellresource.cn/pdf/20080108100822.pdf HL60-cell morphology-SHCRC HL60-细胞形态-SHCRC 3142C0001000000027 Human Acute Promyelocytic Leukemia Cells; HL-60 人急性早幼粒白血病细胞；HL-60 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells were isolated from the peripheral blood of a 36-year-old white woman with acute promyelocytic leukemia by Collins SJ; they can spontaneously differentiate, or differentiate under the stimulation of butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), DMSO(1% to 1.5%), actinomycin D and retinoic acid. TNF-α can be secreted after simulation by PMA. This cell has phagocytosis activity and chemotaxis reaction, oncogene myc-positive, expressing complement receptor and FcR (derived from ATCC). 该细胞由Collins SJ从一位患有急性早幼粒细胞性白血病的36岁白人女性的外周血中分离建立；可自发分化，或在丁酸盐、次黄嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1% to 1.5%)、放线菌素D和视黄酸的刺激下发生分化；PMA刺激后可分泌TNF-α。该细胞具有吞噬活性和趋化反应，癌基因myc阳性，表达补体受体和FcR(来源于ATCC)。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C78 C78 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HL-60 HL-60 cell HL-60 细胞 NICR795 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR795 cell NICR795 细胞 http://www.cellresource.cn/fdetail.aspx?id=795 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3142C0001000000028 Human Acute Promyelocytic Leukemia Cells; HL-60 人急性早幼粒白血病细胞；HL-60 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells were isolated from the peripheral blood of a 36-year-old white woman with acute promyelocytic leukemia by Collins SJ; they can spontaneously differentiate, or differentiate under the stimulation of butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), DMSO(1% to 1.5%), actinomycin D and retinoic acid. TNF-α can be secreted after simulation by PMA. This cell has phagocytosis activity and chemotaxis reaction, oncogene myc-positive, expressing complement receptor and FcR (derived from Institute of Materia Medica). 该细胞由Collins SJ从一位患有急性早幼粒细胞性白血病的36岁白人女性的外周血中分离建立；可自发分化，或在丁酸盐、次黄嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1% to 1.5%)、放线菌素D和视黄酸的刺激下发生分化；PMA刺激后可分泌TNF-α。该细胞具有吞噬活性和趋化反应，癌基因myc阳性，表达补体受体和FcR(来源于药物所)。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C49 C49 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HL-60 HL-60 cell HL-60 细胞 NICR796 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR796 cell NICR796 细胞 http://www.cellresource.cn/fdetail.aspx?id=796 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3111C0002000000098 Human Acute Promyelocytic Leukemia Cells; HL-60 人急性早幼粒细胞白血病细胞；HL-60 medullary 髓样 suspension 悬浮 This cell contains myc+ oncogenic gene, with receptor expression: Complement Fc, and with tumorigenicity: Positive. 该细胞含有myc+致癌基因，受体表达：补体Fc；致瘤性：阳性。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 20%FBS IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 20%FBS http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf The finished list showed that the cell contamination was discarded 完成目录中显示细胞污染废弃 90%FBS+10%DMSO 90%FBS+10%DMSO A类 Class A HL-60-cell morphology-NIFDC HL-60-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net Change fluid once every 2-3 days, and the cell seeding density is 1E + 05/ml, not more than 1E + 06/ml. 每2-3天换液一次，细胞接种密度为1E+05/ml,不要超过1E+06/ml。 HL-60 HL-60 cell HL-60 细胞 NICR2123 NICR2123 cell NICR2123 细胞 http://www.cellresource.cn/fdetail.aspx?id=2123 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf HL-60-cell morphology-NIFDC HL-60-细胞形态-NIFDC melogenin：X；CSF1PO：13；D13S317：13；D16S539：11；D18S51：15，17，18；D19S433：13，14；D21S11：29，31；D2S1338：17，18；D3S1358：15，18；D5S818：11；D7S820：8，9；D8S1179：11，13；FGA：21，24；TH01：8，9.3；TPOX：8，10；vWA：14，18； melogenin：X；CSF1PO：13；D13S317：13；D16S539：11；D18S51：15，17，18；D19S433：13，14；D21S11：29，31；D2S1338：17，18；D3S1358：15，18；D5S818：11；D7S820：8，9；D8S1179：11，13；FGA：21，24；TH01：8，9.3；TPOX：8，10；vWA：14，18； 3111C0001CCC000264 Human Follicular Thyroid Cancer Cells; SW579 [SW 579; SW-579] 人甲状腺鳞癌细胞；SW579 [SW 579；SW-579] epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were derived from squamous cell carcinoma of the thyroid in a 59-year-old white male patient; they were neoplastic in nude mice (producing grade III malignant spindle-like giant cell tumors). 该细胞源于一位59岁的白人男性患者的甲状腺鳞癌组织；在裸鼠中成瘤(产生三级恶性纺锤状巨细胞瘤)。 L15: Leibovitz Medium 10%FBS L15: Leibovitz Medium 10%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 73~80 73~80 A类 Class A SW579-cell morphology-PUMC SW579-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:5~1:10传代，每周换液2~3次。 Passage at 1: 5 ~ 1:10, and change fluid for 2~3 times a week. NICR264 SW 579 SW 579 cell SW 579 细胞 SW-579 SW-579 cell SW-579 细胞 SW579 SW579 cell SW579 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR264 cell NICR264 细胞 http://www.cellresource.cn/fdetail.aspx?id=264 L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf SW579-cell morphology-PUMC SW579-细胞形态-PUMC 3131C0001000700125 Human Follicular Thyroid Cancer Cells; SW579 [SW 579; SW-579] 人甲状腺鳞癌细胞；SW579 [SW 579；SW-579] epithelial cell 上皮细胞 adherent growth 贴壁生长 It forms tumors in nude mice(generating grade III malignant spindle giant cell tumor). 在裸鼠中成瘤(产生三级恶性纺锤状巨细胞瘤)。 L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P(26+5) P(26+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A SW579-cell morphology-SHCRC SW579-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR693 SW 579 SW 579 cell SW 579 细胞 SW-579 SW-579 cell SW-579 细胞 SW579 SW579 cell SW579 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR693 cell NICR693 细胞 http://www.cellresource.cn/fdetail.aspx?id=693 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf SW579-cell morphology-SHCRC SW579-细胞形态-SHCRC 3131C0001001200017 Human Mesothelioma Cells; NCI-H2452 [H2452] 人间皮瘤细胞；NCI-H2452 [H2452] epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 1990年分离建立，细胞来源患者为非吸烟者。 It was isolated and established in 1990, and the patients for cell source was non-smoker. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:4~1:6传代；2~3天换液1次。 Passage at 1: 4 ~ 1: 6; and change fluid once in 2~3 days. H2452 H2452 cell H2452 细胞 NCI-H2452 NCI-H2452 cell NCI-H2452 细胞 NICR1598 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1598 cell NICR1598 细胞 http://www.cellresource.cn/fdetail.aspx?id=1598 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700214 Human Mesothelioma Cells; NCI-H2452 [H2452] 人间皮瘤细胞；NCI-H2452 [H2452] epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell line was established in November 1990. The tissue provider does not smoke. 这株细胞于1990年11月建株。 组织提供者不抽烟。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(17+5) P(17+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 H2452 H2452 cell H2452 细胞 NCI-H2452 NCI-H2452 cell NCI-H2452 细胞 NICR1717 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1717 cell NICR1717 细胞 http://www.cellresource.cn/fdetail.aspx?id=1717 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：11；D13S317：12；D16S539：9，13；D18S51：15，18；D19S433：13，14；D21S11：28，29；D2S1338：17，19；D3S1358：15；D5S818：11，12；D7S820：10，12；D8S1179：11，13；FGA：21，24；TH01：9.3；TPOX：8，11；vWA：14，15； Amelogenin：X；CSF1PO：11；D13S317：12；D16S539：9，13；D18S51：15，18；D19S433：13，14；D21S11：28，29；D2S1338：17，19；D3S1358：15；D5S818：11，12；D7S820：10，12；D8S1179：11，13；FGA：21，24；TH01：9.3；TPOX：8，11；vWA：14，15； 3111C0001CCC000360 Human Plasma Cell Leukemia Cells; NCI-H929 人浆细胞白血病细胞；NCI-H929 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells are derived from the pleural effusion of a 62-year-old man with myeloma, with PCA-1-positive, CD38-positive and EBNA-negative. 该细胞源自一位62岁患有骨髓瘤男性的胸腔积液，PCA-1、CD38阳性，EBNA阴性。 RPMI 1640 (w/o Hepes) 10% FBS；0.05 Mm2-mercaptoethanol RPMI 1640 (w/o Hepes) 10% FBS；0.05 Mm2-巯基乙醇 RPMI 1640 (w/o Hepes) 10% FBS；0.05 Mm2-巯基乙醇 http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C2 C2 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） Permission 近四倍体 A类 Class A NCI-H929-Cell Morphology-PUMC NCI-H929-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell density between 5 � 105 ~ 1 � 106 cells/ml, changing fluid for 2 to 3 times a week 保持细胞密度在5×105～1×106 cells/ml之间，每周换液2～3次 NCI-H929 NCI-H929 cell NCI-H929 细胞 NICR1549 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1549 cell NICR1549 细胞 http://www.cellresource.cn/fdetail.aspx?id=1549 RPMI 1640 (w/o Hepes) 10% FBS；0.05 Mm2-巯基乙醇 URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% FBS；0.05 Mm2-巯基乙醇 URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H929-Cell Morphology-PUMC NCI-H929-细胞形态-PUMC Amelogenin：X，Y； CSF1PO：10，12； D13S317：9，13； D16S539：9，12； D18S51：23； D19S433：11.2，13，14； D21S11：31，33.2； D2S1338：22，24； D3S1358：15，17； D5S818：12，13； D7S820：9，11； D8S1179：13，14； FGA：19，20； TH01：6，9.3； TPOX：8，9； vWA：17，18； Amelogenin：X，Y； CSF1PO：10，12； D13S317：9，13； D16S539：9，12； D18S51：23； D19S433：11.2，13，14； D21S11：31，33.2； D2S1338：22，24； D3S1358：15，17； D5S818：12，13； D7S820：9，11； D8S1179：13，14； FGA：19，20； TH01：6，9.3； TPOX：8，9； vWA：17，18； 3111C0001CCC000467 Human Glioma Cells; GOS-3 人胶质瘤细胞；GOS-3 polygonal 多角 adherent growth 贴壁生长 It is derived from the U-343MG. 由U-343MG衍生而来 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS + 4mM L-Glu MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS + 4mM L-Glu http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；3~4天1次。 Passage at 1: 3; and passage once in 3 to 4 days. GOS-3 GOS-3 cell GOS-3 细胞 NICR2140 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2140 cell NICR2140 细胞 http://www.cellresource.cn/fdetail.aspx?id=2140 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS + 4mM L-Glu URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS + 4mM L-Glu URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000700095 Human Glioblastoma Cells; A172 人胶质母细胞瘤细胞；A172 adherent growth 贴壁生长 Clones are formed in semi-solid medium. It forms no tumor in immunosuppressed mice. 在半固体培养基中形成克隆。 在免疫抑制小鼠中不成瘤。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P311 P311 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 A172 A172 cell A172 细胞 NICR663 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR663 cell NICR663 细胞 http://www.cellresource.cn/fdetail.aspx?id=663 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3131C0001000700171 Human Glioblastoma Cells; A172 人胶质母细胞瘤细胞；A172 adherent growth 贴壁生长 Clones are formed in semi-solid medium. It forms no tumor in immunosuppressed mice. 在半固体培养基中形成克隆。 在免疫抑制小鼠中不成瘤。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P(313+5) P(313+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 A172 A172 cell A172 细胞 NICR2092 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2092 cell NICR2092 细胞 http://www.cellresource.cn/fdetail.aspx?id=2092 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3111C0002000000087 Human Colon Cancer Cells; Caco-2 人结肠癌细胞；Caco-2 epithelioid 上皮样 adherent growth 贴壁生长 The cell doubling time was about 26hrs, and is positive for tumorigenicity, anti-HIV and LAV; The cells express receptors: heat stable enterotoxin, and EGF. 该细胞倍增时间约26h,致瘤性阳性，抗HIV,LAV；受体表达：热稳定肠毒素，EGF。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P21 P21 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Caco-2-cell morphology-NIFDC Caco-2-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:4-1:6，每周换液2-3次 Passage at 1: 4-1: 6, and change fluid for 2-3 times a week Caco-2 Caco-2 cell Caco-2 细胞 NICR2112 NICR2112 cell NICR2112 细胞 http://www.cellresource.cn/fdetail.aspx?id=2112 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Caco-2-cell morphology-NIFDC Caco-2-细胞形态-NIFDC Amelogenin：X；CSF1PO:11；D13S317:11,14；D16S539:12；D18S51:12；D19S433:15；D21S11:30；D2S1338:17,25；D3S1358:17；D5S818:12,13；D7S820:11,12；D8S1179:12；FGA:19；TH01:6；THOX:9,11；vWA:16,18；D3S1358: 17; TH01: 6; D13S317: 11,14; D16S539: 12; D2S1338: 17, 25; D19S433: 15； Amelogenin：X；CSF1PO:11；D13S317:11,14；D16S539:12；D18S51:12；D19S433:15；D21S11:30；D2S1338:17,25；D3S1358:17；D5S818:12,13；D7S820:11,12；D8S1179:12；FGA:19；TH01:6；THOX:9,11；vWA:16,18；D3S1358: 17; TH01: 6; D13S317: 11,14; D16S539: 12; D2S1338: 17, 25; D19S433: 15； 3153C0001000000219 Human Colon Cancer Cells; CaCo-2 人结肠癌细胞；CaCo-2 epithelioid cells 上皮样细胞 adherent growth 贴壁生长 The cell line was isolated from a primary rectal cancer. When the cells were overgrown, they showed typical characteristics of intestinal epithelial cell differentiation. Retinoic acid binding protein I and retinoic acid binding protein II were expressed, and detection for keratin was positive. 该细胞系分离自一个原发性直肠癌。 当细胞长满时，表现出典型的肠上皮细胞分化特征。表达维甲酸结合蛋白I和维甲酸结合蛋白II，角蛋白阳性。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN3 PN3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） None 无 88-98 88-98 A类 Class A Caco-2-cell morphology-KCB Caco-2-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1:2传代；3-4天一次 Passage at 1: 2; and passage once in 3-4 days CaCo-2 CaCo-2 cell CaCo-2 细胞 NICR2170 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2170 cell NICR2170 细胞 http://www.cellresource.cn/fdetail.aspx?id=2170 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 15%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Caco-2-cell morphology-KCB Caco-2-细胞形态-KCB Amelogenin:X；CSF1PO:11,14；D13S317:9,11；D16S539:9；D18S51:12,13；D19S433:12,14.2；D21S11:31；D2S1338:18,19；D3S1358:16,17；D5S818:11,13；D7S820:10,13；D8S1179:15,17；FGA:21,22；TH01:7,8；TPOX:8,12；vWA:18,21 Amelogenin:X；CSF1PO:11,14；D13S317:9,11；D16S539:9；D18S51:12,13；D19S433:12,14.2；D21S11:31；D2S1338:18,19；D3S1358:16,17；D5S818:11,13；D7S820:10,13；D8S1179:15,17；FGA:21,22；TH01:7,8；TPOX:8,12；vWA:18,21 3131C0001000700015 Human Colon Cancer Cells; CW-2 人结肠癌细胞；CW-2 epithelial-like 上皮细胞样 adherent growth 贴壁生长 It is derived from colon cancer. The cells is CEA-positive and transplantation into nude mice can form tumors. 来源于结肠癌。 CEA阳性，移植于裸鼠可成瘤。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 CW-2 CW-2 cell CW-2 细胞 NICR586 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR586 cell NICR586 细胞 http://www.cellresource.cn/fdetail.aspx?id=586 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：11，12；D13S317：8，12；D16S539：12；D18S51：18；D19S433：14；D21S11：29，32.2；D2S1338：18，19，27；D3S1358：14，17；D5S818：12；D7S820：8，12；D8S1179：12；FGA：24，25；TH01：9，9.3；TPOX：8，11；vWA：15，16； Amelogenin：X；CSF1PO：11，12；D13S317：8，12；D16S539：12；D18S51：18；D19S433：14；D21S11：29，32.2；D2S1338：18，19，27；D3S1358：14，17；D5S818：12；D7S820：8，12；D8S1179：12；FGA：24，25；TH01：9，9.3；TPOX：8，11；vWA：15，16； 3111C0001CCC000411 Human Colon Cancer Cells; NCI-H508 人结肠癌细胞；NCI-H508 epithelioid 上皮样 adherent growth 贴壁生长 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A NCI-H508-Cell Morphology-PUMC NCI-H508-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com NCI-H508 NCI-H508 cell NCI-H508 细胞 NICR2030 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2030 cell NICR2030 细胞 http://www.cellresource.cn/fdetail.aspx?id=2030 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H508-Cell Morphology-PUMC NCI-H508-细胞形态-PUMC Amelogenin:X；CSF1PO:8,10；D13S317:8,11；D16S539:13；D18S51:11,12；D19S433:14；D21S11:27,30；D2S1338:16；D3S1358:16,19；D5S818:11,13；D7S820:8,10；D8S1179:9,13,14；FGA:20,21,22,23；TH01:6,10；TPOX:11；vWA:16,22 Amelogenin:X；CSF1PO:8,10；D13S317:8,11；D16S539:13；D18S51:11,12；D19S433:14；D21S11:27,30；D2S1338:16；D3S1358:16,19；D5S818:11,13；D7S820:8,10；D8S1179:9,13,14；FGA:20,21,22,23；TH01:6,10；TPOX:11；vWA:16,22 3131C0001000700116 Human Colon Cancer Cells; RKO 人结肠癌细胞；RKO epithelial cell 上皮细胞 adherent growth 贴壁生长 RKO is a poorly differentiated colon cancer cell line. RKO cells contain wild-type P53 but lack human thyroid receptor nuclear receptor (h-TR beta 1). The level of P53 protein in RKO cells is higher than that in RKO-E6 cells. The RKO cell line is the parent cell line of RKO-E6 and RKO-AS45-1. This cell line form tumors in nude mice and forms colonies in soft agar. RKO是一个低分化的结肠癌细胞系。 RKO细胞含有野生型P53，但缺乏人甲状腺受体核受体(h-TR beta 1)。 RKO细胞的P53蛋白的水平高于RKO-E6细胞。 RKO细胞系是RKO-E6和RKO-AS45-1的亲本细胞系。 该细胞系在裸鼠中成瘤，且在软琼脂中形成集落。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A RKO-DNA-Fingerprinting-SHCRC RKO-DNA-指纹-SHCRC RKO-cell morpholgoy-SHCRC RKO-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR684 RKO RKO cell RKO 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR684 cell NICR684 细胞 http://www.cellresource.cn/fdetail.aspx?id=684 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf RKO-DNA-Fingerprinting-SHCRC RKO-DNA-指纹-SHCRC RKO-cell morpholgoy-SHCRC RKO-细胞形态-SHCRC 3131C0001000700117 Human Colon Cancer Transgenic Cells; RKO-AS45-1 人结肠癌转基因细胞；RKO-AS45-1 epithelial cell 上皮细胞 adherent growth 贴壁生长 The open reading frame encoding GADD45 in human cDNA was cloned into expression vector pCMV. 3, and the RKO-AS45-1 cell line was established by transfected RKO with the generated vetor. The cell contains an expression vector that stably integrates a cytomegalovirus (CMV) promoter. The RKO-AS45-1 cell line can be used together with its maternal RKO for Gadd45 studies on DNA repair, apoptosis and drug sensitivity. 人的cDNA中编码GADD45的开放阅读框克隆到表达载体pCMV.3中，转染结肠癌细胞RKO，建立了RKO-AS45-1细胞株。 该细胞含有稳定整合巨细胞病毒（CMV）启动子的表达载体。 RKO-AS45-1细胞系和它的母系RKO一起可用于Gadd45对DNA修复、凋亡和药物敏感性研究。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR685 RKO-AS45-1 RKO-AS45-1 cell RKO-AS45-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR685 cell NICR685 细胞 http://www.cellresource.cn/fdetail.aspx?id=685 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000700118 Human Colon Cancer Transgenic Cells; RKO-E6 人结肠癌转基因细胞；RKO-E6 epithelial cell 上皮细胞 adherent growth 贴壁生长 RKO-E6细胞系是用脂质体法转染pCMV-E6的结肠癌RKO细胞系。 该细胞含有稳定整合受控於巨细胞病毒启动子的人乳头状病毒（HPV）E6癌基因。 RKO-E6细胞系和它的母系RKO一起可用于研究p53缺失引起的细胞参数改变p53介导的转录和凋亡等。 The RKO-E6 cell line is a colon cancer RKO cell line transfected with pCMV-E6 by liposome method. The cells contain a human papillomavirus (HPV) E6 oncogene that is stably integrated and controled by cytomegalovirus promoters. The RKO-E6 cell line, together with its maternal line RKO, can be used to study the p53 deletion-induced changes in cell parameters such as p53-mediated transcription and apoptosis. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR686 RKO-E6 RKO-E6 cell RKO-E6 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR686 cell NICR686 细胞 http://www.cellresource.cn/fdetail.aspx?id=686 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000700174 Human Colon Adenocarcinoma Cells; SW1116 人结肠腺癌细胞；SW1116 epithelial cell 上皮细胞 adherent growth 贴壁生长 CSAp阴性(CSAp-)。 结肠抗原3，阴性。 角蛋白免疫过氧化物酶染色阳性。 癌基因c-myc, K-ras, H-ras, myb, sis 和fos的表达呈阳性。 未检测到癌基因N-myc和N-ras的表达。 表达肿瘤特异的核基质蛋白CC-4，CC-5和CC-６。 Test for CSAp is negative (CSAp -). Test for Colon antigen 3 is negative. The cells are positive for keratin by immunoperoxidase staining. The expressions of oncogene c-myc, K-ras, H-ras, myb, sis and fos are positive. The expression of oncogene N-myc and N-ras is not detected. The cells express tumor-specific nuclear matrix proteins CC-4, CC-5 and CC-6. L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P(41+5) P(41+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1685 SW1116 SW1116 cell SW1116 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1685 cell NICR1685 细胞 http://www.cellresource.cn/fdetail.aspx?id=1685 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf STR Identification STR鉴定 3142C0001000000332 Human Colon Adenocarcinoma Cells; SW48 人结肠腺癌细胞；SW48 epithelial-like 上皮细胞样 adherent growth 贴壁生长 1971-1975年间，A. Leibovitz从结肠腺癌中分离建立十个细胞系，SW48为为其中之一。该细胞合成癌胚抗原，能在裸鼠中成瘤。 From 1971 to 1975, A. Leibovitz isolated and established ten cell lines from colon adenocarcinoma, one of which was SW48. This cell synthesized carcinoembryonic antigen, which can form tumors in nude mice. L15: Leibovitz Medium 10%FBS L15: Leibovitz Medium 10%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf C8 C8 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR1983 SW48 SW48 cell SW48 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1983 cell NICR1983 细胞 http://www.cellresource.cn/fdetail.aspx?id=1983 L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf 3131C0001001200009 Human Colorectal Cancer Lung Metastases Cells; T84 人结直肠癌肺转移细胞；T84 epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 The cells express neurotransmitter receptor and peptide hormone receptor. 表达神经递质受体和肽类激素受体 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:3传代；3-4天一次　 Passage at 1: 3; and passage once in 3-4 days NICR1590 T84 T84 cell T84 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1590 cell NICR1590 细胞 http://www.cellresource.cn/fdetail.aspx?id=1590 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf 3131C0001000700211 Human Colorectal Cancer Lung Metastases Cells; T84 人结直肠癌肺转移细胞；T84 epithelial cell 上皮细胞 adherent growth 贴壁生长 T84 cell line is a transplantable human cancer cell line established from the lung metastasis of a 72-year-old male patient with colon cancer. Tumor tissue was inoculated subcutaneously into BALB/c nude mice and transplanted continuously. [26072］During the transplantation of nude mice, the cell lines always maintained the original tissue characteristics of colon cancer. [26072］T84 cell line was established after 23 passages in thymic-free mice. These cell monolayers grew to saturation and exhibited tight junctions and desmosomes between adjacent cells. [1155] There are a lot of reports about polypeptide hormones and neurotransmitters and maintaining directional electrolyte transport. [1155］This cell line presents tight junctions and desmosomes between adjacent cells. [1155］ The cells are keratin-positive in Immunoperoxide staining. T84细胞株是从一位72岁男性结肠癌患者的肺转移灶建立的可移植人类癌细胞株。 肿瘤组织皮下接种于BALB/c裸鼠，并连续进行移植。 [26072］ 在裸鼠身上的移植过程中，细胞株始终保持结肠癌的原始组织性状。 [26072］ 在无胸腺小鼠中传代23代后建立了T84细胞株。 这些细胞单层生长到饱和并在接触细胞间展现出紧密连接和桥粒。 [1155] 有很多关于多肽类激素和神经递质并维持定向电解质传输的报道。 [1155］ 这株细胞展现了接触细胞中的紧密连接和桥粒。 [1155］ 角蛋白免疫过氧化物 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) qualified fetal bovine serum，5% DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，5% DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，5% http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf P(53+5) P(53+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1715 T84 T84 cell T84 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1715 cell NICR1715 细胞 http://www.cellresource.cn/fdetail.aspx?id=1715 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，5% URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，5% URI: http://www.cellresource.cn/pdf/20080108100542.pdf Amelogenin：X；CSF1PO：10；D13S317：9；D16S539：10，11；D18S51：17；D19S433：13；D21S11：31；D2S1338：23，26；D3S1358：19；D5S818：12；D7S820：8，10；D8S1179：15；FGA：24；TH01：6，9；TPOX：8，11；vWA：17，18； Amelogenin：X；CSF1PO：10；D13S317：9；D16S539：10，11；D18S51：17；D19S433：13；D21S11：31；D2S1338：23，26；D3S1358：19；D5S818：12；D7S820：8，10；D8S1179：15；FGA：24；TH01：6，9；TPOX：8，11；vWA：17，18； 3111C0001CCC000056 Human Colorectal Cancer Cells; T84 人结直肠癌细胞；T84 epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 T84 cells were derived from the lung metastasis of a 72-year-old male patient with colorectal cancer; the cell line was established after subcutaneous inoculation into BALB/c nude mice and serial passage for 23 times. During passage in nude mice, the tumor still retained the histological characteristics of the original colorectal cancer. When T84 cell line grows to confluent state, it forms monolayer cells with tight junction and cell desmosomes between adjacent cells; the cells have receptors for various peptide hormones and neurotransmitters and maintain the directional transport of electrolytes; the cells are keratin-positive. T84细胞来源于一位72岁的男性结直肠癌患者的肺部转移灶；皮下接种至BALB/c裸鼠并连续传代23次后建立该细胞系。在裸鼠身上传代的过程中，该肿瘤仍保持原始的结直肠癌的组织学特性。T84细胞系生长至汇合状态时形成单层细胞，相邻细胞间有紧密连接和细胞桥粒；该细胞有多种肽类激素和神经递质的受体，维持电解质的定向运输；角蛋白阳性。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 51~60 51~60 A类 Class A T84-Species Identification-PUMC T84-cell morphology-PUMC T84-物种鉴定-PUMC T84-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；每周2次。 Passage at 1: 2 ~ 1: 4; and passage for 2 times a week. NICR56 T84 T84 cell T84 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR56 cell NICR56 细胞 http://www.cellresource.cn/fdetail.aspx?id=56 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf T84-Species Identification-PUMC T84-cell morphology-PUMC T84-物种鉴定-PUMC T84-细胞形态-PUMC - - 3111C0001CCC000331 Human Colorectal Adenocarcinoma Cells; HCT 116 [HCT116] 人结直肠腺癌瘤株；HCT 116 [HCT116] solid tumor 实体瘤 growth in vivo 体内生长 Nude mice were transplanted in vivo with the cultured cells to form solid tumor, which was cryopreserved and can be used to establish the transplantation model of human colorectal cancer in vivo. 利用培养的该细胞系进行裸鼠体内移植，形成实体瘤，冻存；可用于建立人结直肠癌的体内移植模型。 - - P1 P1 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） - - A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Cells were prepared into cell suspension and inoculated into immunodeficient mice. 制备成细胞悬液接种至免疫缺陷小鼠。 HCT 116 HCT 116 cell HCT 116 细胞 HCT116 HCT116 cell HCT116 细胞 NICR331 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR331 cell NICR331 细胞 http://www.cellresource.cn/fdetail.aspx?id=331 Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,11；D16S539:12,13；D18S51:11,17；D19S433:14,16；D21S11:29,32.2；D2S1338:17,25；D3S1358:17；D5S818:13；D7S820:10,12；D8S1179:15；FGA:22；TH01:7,9.3；TPOX:8,11；vWA:18,19 Amelogenin:X,Y；CSF1PO:11,12；D13S317:8,11；D16S539:12,13；D18S51:11,17；D19S433:14,16；D21S11:29,32.2；D2S1338:17,25；D3S1358:17；D5S818:13；D7S820:10,12；D8S1179:15；FGA:22；TH01:7,9.3；TPOX:8,11；vWA:18,19 3131C0001000700134 Human Epithelial Colorectal Adenocarcinoma Cells; DLD-1 人结直肠腺癌上皮细胞；DLD-1 epithelial cell 上皮细胞 adherent growth 贴壁生长 DLD-1 was one of the two strains of colorectal adenocarcinoma cell lines isolated by D.L. Dexter and his colleagues. DNA fingerprinting and karyotype analysis performed at ATCC and elsewhere showes that this cell line is similar to HCT-15 (CCL-225), suggesting that the two were different clones from the same person. Their genetic origins can be confirmed by DNA fingerprinting, but karyotype analysis shows that they lack a consistent change in chromosome markers or number. The cells was negative in CSAp(CSAP-)test. The expression of p53 antigen is positive in DLD-1 cells (a C-> T mutation in the p53 antigen resulted in the serine-> phenylalanine transition at position 241) DLD-1 是1977-1979年间D.L. Dexter和同事分离的两株结直肠腺癌细胞株中的一株。 在ATCC和其它地方进行的DNA fingerprinting和染色体组型分析表明这株细胞与HCT-15 (CCL-225)相似，说明这两者是来自同一个人的不同克隆。 他们的遗传起源可通过DNA fingerprinting证实，但染色体组型分析显示它们缺乏染色体标记一致改变或数目上一致改变。 细胞的CSAp阴性(CSAp-)。 DLD-1 细胞的 p53 抗原表达呈阳性(p53 抗原产生了一个C -> RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A DLD-1-cell morphology-SHCRC DLD-1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 DLD-1 DLD-1 cell DLD-1 细胞 NICR702 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR702 cell NICR702 细胞 http://www.cellresource.cn/fdetail.aspx?id=702 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf DLD-1-cell morphology-SHCRC DLD-1-细胞形态-SHCRC Amelogenin：X；CSF1PO：11；D13S317：11，13，14；D16S539：12，13；D18S51：12；D19S433：15；D21S11：30，32；D2S1338：17，19.2，25；D3S1358：14，17；D5S818：12，13；D7S820：11，12；D8S1179：12，14；FGA：19；TH01：6；TPOX：9，11；vWA：16，18； Amelogenin：X；CSF1PO：11；D13S317：11，13，14；D16S539：12，13；D18S51：12；D19S433：15；D21S11：30，32；D2S1338：17，19.2，25；D3S1358：14，17；D5S818：12，13；D7S820：11，12；D8S1179：12，14；FGA：19；TH01：6；TPOX：9，11；vWA：16，18； 3111C0001CCC000100 Human Colorectal Adenocarcinoma Cells; Caco-2 人结直肠腺癌细胞；Caco-2 epithelioid 上皮样 adherent growth 贴壁生长 This cell line was isolated from a primary colon cancer. When the cells grow to fully confluence, they show typical characteristics of intestinal cell differentiation. Caco-2 cells express tretinoin binding protein I and retinol binding protein II, and are keratin-positive. 此细胞株分离自一个原发性结肠癌。当细胞长满时，表现出典型的肠细胞分化的特征。Caco-2细胞表达维生素A酸结合蛋白I和视黄醇结合蛋白II，角蛋白阳性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 20%FBS；1%NEAA+10mM Hepes MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 20%FBS；1%NEAA+10mM Hepes http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P27 P27 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 85~90，91~100 85~90，91~100 A类 Class A Caco-2-Species Identification-PUMC Caco-2-cell morphology-PUMC Caco-2-物种鉴定-PUMC Caco-2-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:6传代，细胞达80%汇合时进行传代；每周1~2次换液。 Passage at 1: 4 ~ 1: 6, and passage when 80% of the cells are in confluence; and change fluid for 1 ~ 2 times a week. Caco-2 Caco-2 cell Caco-2 细胞 NICR100 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR100 cell NICR100 细胞 http://www.cellresource.cn/fdetail.aspx?id=100 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 20%FBS；1%NEAA+10mM Hepes URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 20%FBS；1%NEAA+10mM Hepes URI: http://www.cellresource.cn/pdf/20080108100951.pdf Caco-2-Species Identification-PUMC Caco-2-cell morphology-PUMC Caco-2-物种鉴定-PUMC Caco-2-细胞形态-PUMC Amelogenin:X；CSF1PO:10；D13S317:8,12；D16S539:11,12；D18S51:17；D19S433:15,16；D21S11:30；D2S1338:21；D3S1358:15；D5S818:13；D7S820:8,11；D8S1179:15；FGA:21；TH01:7；TPOX:8；vWA:17 Amelogenin:X；CSF1PO:10；D13S317:8,12；D16S539:11,12；D18S51:17；D19S433:15,16；D21S11:30；D2S1338:21；D3S1358:15；D5S818:13；D7S820:8,11；D8S1179:15；FGA:21；TH01:7；TPOX:8；vWA:17 3131C0001000700066 Human Colorectal Adenocarcinoma Cells; Caco-2 人结直肠腺癌细胞；Caco-2 epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 This cell line was isolated from a primary colon cancer. When the cells grow to fully confluence, they show typical characteristics of intestinal cell differentiation. Caco-2 cells express tretinoin binding protein I and retinol binding protein II, and are keratin-positive. 此细胞株分离自一个原发性结肠癌。当细胞长满时，表现出典型的肠细胞分化的特征。Caco-2细胞表达维生素A酸结合蛋白I和视黄醇结合蛋白II，角蛋白阳性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 20%FBS；1%NEAA+10mM Hepes MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 20%FBS；1%NEAA+10mM Hepes http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Caco-2-cell morphology-SHCRC Caco-2-细胞形态-SHCRC LDH检测报告Caco-2-Isoenzyme-SHCRC LDH检测报告Caco-2-同工酶-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:4~1:6传代，细胞达80%汇合时进行传代；每周1~2次换液。 Passage at 1: 4 ~ 1: 6, and passage when 80% of the cells are in confluence; and change fluid for 1 ~ 2 times a week. Caco-2 Caco-2 cell Caco-2 细胞 NICR634 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR634 cell NICR634 细胞 http://www.cellresource.cn/fdetail.aspx?id=634 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 20%FBS；1%NEAA+10mM Hepes URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 20%FBS；1%NEAA+10mM Hepes URI: http://www.cellresource.cn/pdf/20080108100951.pdf Caco-2-cell morphology-SHCRC Caco-2-细胞形态-SHCRC LDH检测报告Caco-2-Isoenzyme-SHCRC LDH检测报告Caco-2-同工酶-SHCRC Amelogenin:X；CSF1PO:10；D13S317:8,12；D16S539:11,12；D18S51:17；D19S433:15,16；D21S11:30；D2S1338:21；D3S1358:15；D5S818:13；D7S820:8,11；D8S1179:15；FGA:21；TH01:7；TPOX:8；vWA:17 Amelogenin:X；CSF1PO:10；D13S317:8,12；D16S539:11,12；D18S51:17；D19S433:15,16；D21S11:30；D2S1338:21；D3S1358:15；D5S818:13；D7S820:8,11；D8S1179:15；FGA:21；TH01:7；TPOX:8；vWA:17 3131C0001000700146 Human Colorectal Adenocarcinoma Cells; Caco-2 人结直肠腺癌细胞；Caco-2 epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell line was isolated from rectal carcinoma in situ. When the cells grow to confluence, they show characteristic differentiation of intestinal epithelial cells. Caco-2 cells express tretinoin binding protein I and tretinoin binding protein II, and are keratin-positive. 这株细胞分离自直肠原位癌。 当长到满时，细胞表现出特征性的肠上皮细胞分化。 Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白II，并呈角质蛋白阳性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(17+5) P(17+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Caco-2-cell morphology-SHCRC Caco-2-细胞形态-SHCRC LDH检测报告Caco-2-Isoenzyme-SHCRC LDH检测报告Caco-2-同工酶-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 15-20 minutes. Passage at 1: 2. The cells can be overgrowth in 4-5 days. 消化15-20分钟。1:2。4-5天长满。 Caco-2 Caco-2 cell Caco-2 细胞 NICR712 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR712 cell NICR712 细胞 http://www.cellresource.cn/fdetail.aspx?id=712 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf Caco-2-cell morphology-SHCRC Caco-2-细胞形态-SHCRC LDH检测报告Caco-2-Isoenzyme-SHCRC LDH检测报告Caco-2-同工酶-SHCRC STR Identification STR鉴定 3142C0001000000137 Human Colorectal Adenocarcinoma Cells; Caco-2 人结直肠腺癌细胞；Caco-2 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line was isolated from a primary colon cancer. When the cells grow to fully confluence, they show typical characteristics of intestinal cell differentiation. Caco-2 cells express tretinoin binding protein I and retinol binding protein II, and are keratin-positive. 此细胞株分离自一个原发性结肠癌。当细胞长满时，表现出典型的肠细胞分化的特征。Caco-2细胞表达维生素A酸结合蛋白I和视黄醇结合蛋白II，角蛋白阳性。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C21 C21 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Caco-2 Caco-2 cell Caco-2 细胞 NICR905 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR905 cell NICR905 细胞 http://www.cellresource.cn/fdetail.aspx?id=905 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin：X；CSF1PO：11，12；D13S317：10，12；D16S539：12，13；D18S51：18；D19S433：13，14；D21S11：30.2，33.2；D2S1338：17，18；D3S1358：16；D5S818：10，13；D7S820：9，10；D8S1179：9，14；FGA：21，23；TH01：8，9；TPOX：11；vWA：15； Amelogenin：X；CSF1PO：11，12；D13S317：10，12；D16S539：12，13；D18S51：18；D19S433：13，14；D21S11：30.2，33.2；D2S1338：17，18；D3S1358：16；D5S818：10，13；D7S820：9，10；D8S1179：9，14；FGA：21，23；TH01：8，9；TPOX：11；vWA：15； 3111C0001CCC000340 Human Colorectal Adenocarcinoma Cells; COLO 201 人结直肠腺癌细胞；COLO 201 fibroblast-like, bipolar 成纤维细胞样，双极 suspension growth, loosely adherent 悬浮生长，松散贴壁 The cells are derived from a 70-year-old white male and are CSAp (CSAP-) and CEA-negative. 该细胞源自一位70岁白人男性，CSAp (CSAp-)和CEA阴性。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） Permission 超三倍体 A类 Class A Colo 201-Cell Morphology-PUMC Colo 201-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:10传代，每周换液1～2次 Passage at 1: 2 ~ 1:10, and change fluid for 1 to 2 times a week COLO 201 COLO 201 cell COLO 201 细胞 NICR1529 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1529 cell NICR1529 细胞 http://www.cellresource.cn/fdetail.aspx?id=1529 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Colo 201-Cell Morphology-PUMC Colo 201-细胞形态-PUMC Amelogenin：X；CSF1PO：11，12；D13S317：10；D16S539：12，13；D18S51：18；D19S433：13，14；D21S11：30.2，33.2；D2S1338：17，18；D3S1358：16；D5S818：10，13；D7S820：9，10；D8S1179：9，14；FGA：21，23；TH01：8，9；TPOX：11；vWA：15； Amelogenin：X；CSF1PO：11，12；D13S317：10；D16S539：12，13；D18S51：18；D19S433：13，14；D21S11：30.2，33.2；D2S1338：17，18；D3S1358：16；D5S818：10，13；D7S820：9，10；D8S1179：9，14；FGA：21，23；TH01：8，9；TPOX：11；vWA：15； 3111C0001CCC000132 Human Colorectal Adenocarcinoma Cells; COLO 205 人结直肠腺癌细胞；COLO 205 epithelioid 上皮样 adherent growth; suspension growth 贴壁生长;悬浮生长 The cell line was isolated from the ascites of a 70-year-old male white man with colon cancer in 1957 by T.U. Sample et al. The patient was treated with 5-fluorouracil for 4-6 weeks before taking the ascites sample. Keratin immunoperoxidase staining was positive; CEA and IL10 were produced. 该细胞系是1957年由T.U.Sample等从患有结肠癌的70岁男性白人的腹水中分离的。 该病人在取腹水样品前已用5-氟尿嘧啶治疗4~6周。角蛋白免疫过氧化物酶染色阳性；产生CEA、IL10。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C6 C6 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 71~75 71~75 A类 Class A Colo 205-Cell Morphology-PUMC Colo 205-Species Identification-PUMC Colo 205-物种鉴定-PUMC Colo 205-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:6传代；2~3天换液1次。 Passage at 1: 2 ~ 1: 6; and change fluid once in 2~3 days. COLO 205 COLO 205 cell COLO 205 细胞 NICR132 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR132 cell NICR132 细胞 http://www.cellresource.cn/fdetail.aspx?id=132 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Colo 205-Cell Morphology-PUMC Colo 205-Species Identification-PUMC Colo 205-物种鉴定-PUMC Colo 205-细胞形态-PUMC Amelogenin:X；CSF1PO:11,12；D13S317:10,12；D16S539:12,13；D18S51:18；D19S433:13,14；D21S11:30.2,33.2；D2S1338:17,18；D3S1358:16；D5S818:10,13；D7S820:9,10；D8S1179:9,14；FGA:21,23；TH01:8,9；TPOX:11；vWA:15 Amelogenin:X；CSF1PO:11,12；D13S317:10,12；D16S539:12,13；D18S51:18；D19S433:13,14；D21S11:30.2,33.2；D2S1338:17,18；D3S1358:16；D5S818:10,13；D7S820:9,10；D8S1179:9,14；FGA:21,23；TH01:8,9；TPOX:11；vWA:15 3131C0001000700102 Human Colorectal Adenocarcinoma Cells; COLO 205 人结直肠腺癌细胞；COLO 205 epithelioid 上皮样 adherent growth 贴壁生长 This cell line was isolated from the ascites of a 70-year-old white male with colon cancer in 1957 by T.U. Sample et al. The patient was treated with 5-fluorouracil for 4-6 weeks before taking the ascites sample. The cells were keratin-positive for immunoperoxidase staining. 该细胞系1957年由T.U.Sample等从患有结肠癌的70岁男性白人的腹水分离。 该病人在取腹水样品前已用5-氟尿嘧啶治疗4-6周。 角蛋白免疫过氧化物酶染色阳性。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Colo 205-cell morphology-SHCRC Colo 205-细胞形态-SHCRC Colo-205-DNA-Fingerprinting-SHCRC Colo-205-DNA-指纹-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 COLO 205 COLO 205 cell COLO 205 细胞 NICR670 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR670 cell NICR670 细胞 http://www.cellresource.cn/fdetail.aspx?id=670 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Colo 205-cell morphology-SHCRC Colo 205-细胞形态-SHCRC Colo-205-DNA-Fingerprinting-SHCRC Colo-205-DNA-指纹-SHCRC Amelogenin：X；CSF1PO：11；D13S317：11；D16S539：11，12；D18S51：15；D19S433：16.2；D21S11：33.2；D2S1338：18，25；D3S1358：17；D5S818：12；D7S820：9，12；D8S1179：13；FGA：20；TH01：9；TPOX：8，9；vWA：15，18； Amelogenin：X；CSF1PO：11；D13S317：11；D16S539：11，12；D18S51：15；D19S433：16.2；D21S11：33.2；D2S1338：18，25；D3S1358：17；D5S818：12；D7S820：9，12；D8S1179：13；FGA：20；TH01：9；TPOX：8，9；vWA：15，18； 3111C0001CCC000133 Human Colorectal Adenocarcinoma Cells; COLO 320DM [COLO320DM] 人结直肠腺癌细胞；COLO 320DM [COLO320DM] round 圆形 adherent growth; suspension growth 贴壁生长;悬浮生长 This cell can produce serotonin, norepinephrine, adrenaline, ACTH and parathyroid hormone. Detections for keratin and vimentin are weakly positive. 该细胞可产生5-羟色胺、去甲肾上腺素、肾上腺素、ACTH和甲状旁腺素。角蛋白、波形蛋白弱阳性。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 49~52 49~52 A类 Class A Colo320 DM-Species Identification-PUMC Colo320 DM-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:5~1:10传代；2~3天换液1次。 Passage at 1: 5 ~ 1:10; and change fluid once in 2~3 days. COLO 320DM COLO 320DM cell COLO 320DM 细胞 COLO320DM COLO320DM cell COLO320DM 细胞 NICR133 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害。 NICR133 cell NICR133 细胞 http://www.cellresource.cn/fdetail.aspx?id=133 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Colo320 DM-Species Identification-PUMC Colo320 DM-物种鉴定-PUMC 3131C0001000700014 Human Colorectal Adenocarcinoma Cells; COLO 320DM [COLO320DM] 人结直肠腺癌细胞；COLO 320DM [COLO320DM] Others 其它 adherent growth 贴壁生长 The cells can synthesize serotonin. 能合成复合胺，肾上腺素。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 COLO 320DM COLO 320DM cell COLO 320DM 细胞 COLO320DM COLO320DM cell COLO320DM 细胞 NICR585 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR585 cell NICR585 细胞 http://www.cellresource.cn/fdetail.aspx?id=585 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001000700208 Human Colorectal Adenocarcinoma Cells; COLO 320DM [COLO320DM] 人结直肠腺癌细胞；COLO 320DM [COLO320DM] round and refractive 圆形的并能折光的 semi-adherent growth 半贴壁生长 Tests for CEA, CSAp and colon antigen 3 were negative in this cell line. Tetst for keratin and vimentin were weakly positive. 这株细胞CEA、CSAp和结肠抗原3呈阴性。 角蛋白和波形纖維蛋白呈弱阳性 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. COLO 320DM COLO 320DM cell COLO 320DM 细胞 COLO320DM COLO320DM cell COLO320DM 细胞 NICR1713 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1713 cell NICR1713 细胞 http://www.cellresource.cn/fdetail.aspx?id=1713 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf AMEL:X,Y；CSF1PO:11,12；D12S391:19,22；D13S317:8,11；D16S539:12,13；D18S51:11,17；D19S433:14,16；D21S11:29,32.2；D2S1338:17,25；D2S441:13,15；D3S1358:17,17；D5S818:13,13；D6S1043:11,13；D7S820:10,12；D8S1179:15,15；FGA:22,22；Penta D:9,14；Penta E:7,14；TH01:7,9.3；TPOX:8,1 AMEL:X,Y；CSF1PO:11,12；D12S391:19,22；D13S317:8,11；D16S539:12,13；D18S51:11,17；D19S433:14,16；D21S11:29,32.2；D2S1338:17,25；D2S441:13,15；D3S1358:17,17；D5S818:13,13；D6S1043:11,13；D7S820:10,12；D8S1179:15,15；FGA:22,22；Penta D:9,14；Penta E:7,14；TH01:7,9.3；TPOX:8,1 3142C0001000000963 Human Colorectal Adenocarcinoma Cells; DLD-1 人结直肠腺癌细胞；DLD-1 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell is suitable for use as transfected hosts. 该细胞适于做转染宿主 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. DLD-1 DLD-1 cell DLD-1 细胞 NICR2200 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2200 cell NICR2200 细胞 http://www.cellresource.cn/fdetail.aspx?id=2200 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X，Y；CSF1PO：7，10；D13S317：10，12；D16S539：11，13；D18S51：16，17；D19S433：12，13；D21S11：29，30；D2S1338：16；D3S1358：12，18，19；D5S818：10，11；D7S820：11，12；D8S1179：12，14；FGA：18，23；TH01：8，9；TPOX：8；vWA：17，22； Amelogenin：X，Y；CSF1PO：7，10；D13S317：10，12；D16S539：11，13；D18S51：16，17；D19S433：12，13；D21S11：29，30；D2S1338：16；D3S1358：12，18，19；D5S818：10，11；D7S820：11，12；D8S1179：12，14；FGA：18，23；TH01：8，9；TPOX：8；vWA：17，22； 3111C0001CCC000158 Human Colorectal Adenocarcinoma Cells; HCT 116 [HCT116] 人结直肠腺癌细胞；HCT 116 [HCT116] epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 HCT116 is one of three malignant cells isolated from a male patient with colon cancer by Brattain M in 1979, producing CEA and keratin. It forms clones in semi-solid agarose medium. HCT116 is tumorigenic in thymic-free nude mice and forms epithelioid tumors. HCT116是1979年Brattain M等从患结肠癌的男性病人中分离建立的3株恶性细胞之一；产生CEA、角蛋白。在半固体琼脂糖培养基中形成克隆。HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 43~46，XY 43~46，XY A类 Class A HCT116-Species Identification-PUMC HCT116-cell morphology-PUMC HCT116-物种鉴定-PUMC HCT116-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；3~4天1次。 Passage at 1: 3; and passage once in 3 to 4 days. HCT 116 HCT 116 cell HCT 116 细胞 HCT116 HCT116 cell HCT116 细胞 NICR158 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR158 cell NICR158 细胞 http://www.cellresource.cn/fdetail.aspx?id=158 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf HCT116-Species Identification-PUMC HCT116-cell morphology-PUMC HCT116-物种鉴定-PUMC HCT116-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:7,10；D13S317:10,12；D16S539:11,13；D18S51:17；D19S433:12,13；D21S11:29,30；D2S1338:16；D3S1358:12,17,18,19；D5S818:10,11；D7S820:11,12；D8S1179:11,12,14；FGA:18,23；TH01:8,9；TPOX:8,9；vWA:17,22 Amelogenin:X,Y；CSF1PO:7,10；D13S317:10,12；D16S539:11,13；D18S51:17；D19S433:12,13；D21S11:29,30；D2S1338:16；D3S1358:12,17,18,19；D5S818:10,11；D7S820:11,12；D8S1179:11,12,14；FGA:18,23；TH01:8,9；TPOX:8,9；vWA:17,22 3131C0001000700099 Human Colorectal Adenocarcinoma Cells; HCT 116 [HCT116] 人结直肠腺癌细胞；HCT 116 [HCT116] epithelium 上皮 adherent growth 贴壁生长 HCT116 is one of three malignant cells isolated from a male patient with colon cancer by M. Brattain et al in 1979. It forms clones in semi-solid agarose medium. HCT116 is tumorigenic in thymic-free nude mice and forms epithelioid tumors. HCT116是1979年M.Brattain等从患结肠癌的男性病人中分离的三株恶性细胞之一。 在半固体琼脂糖培养基中形成克隆。 HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。 McCoy＇s 5A Media (Modified with Tricine) qualified fetal bovine serum，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P(9+5) P(9+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A HCT116-cell morphology-SHCRC HCT116-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion 5-10 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化5-10分钟。1:2。3天内可长满。 HCT 116 HCT 116 cell HCT 116 细胞 HCT116 HCT116 cell HCT116 细胞 NICR667 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR667 cell NICR667 细胞 http://www.cellresource.cn/fdetail.aspx?id=667 McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf HCT116-cell morphology-SHCRC HCT116-细胞形态-SHCRC Amelogenin：X，Y；CSF1PO：12；D13S317：8，11；D16S539：12，13；D18S51：11，17；D19S433：14，16；D21S11：29，32.2；D2S1338：17，25；D3S1358：17；D5S818：13；D7S820：10，12；D8S1179：15；FGA：22；TH01：7，9.3；TPOX：8，11；vWA：18，19； Amelogenin：X，Y；CSF1PO：12；D13S317：8，11；D16S539：12，13；D18S51：11，17；D19S433：14，16；D21S11：29，32.2；D2S1338：17，25；D3S1358：17；D5S818：13；D7S820：10，12；D8S1179：15；FGA：22；TH01：7，9.3；TPOX：8，11；vWA：18，19； 3111C0001CCC000247 Human Colorectal Adenocarcinoma Cells; HCT-15 [HCT15] 人结直肠腺癌细胞；HCT-15 [HCT15] epithelioid 上皮样 adherent growth 贴壁生长 DNA fingerprinting证据表明，这株细胞和DLD-1(ATCC CCL-221,人结直肠腺癌)来源于同一个人；但同工酶及细胞染色体组型分析仍存疑问；细胞呈CSAp阴性(CSAp-)；角蛋白阳性。 The evidence of DNA fingerprinting showes that this cell line is derived from the same person as DLD-1 (ATCC CCL-221, human colorectal adenocarcinoma), but the analysis of isozymes and cell karyotype remained questionable. The cells are negative in test for CSAp(CSAP-). It is positive in immunoperoxidase staining. RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:10传代；每周换液2~3次。 Passage at 1: 2 ~ 1:10; and change fluid for 2~3 times a week. HCT-15 HCT-15 cell HCT-15 细胞 HCT15 HCT15 cell HCT15 细胞 NICR247 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR247 cell NICR247 细胞 http://www.cellresource.cn/fdetail.aspx?id=247 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X，Y；CSF1PO：12；D13S317：8，11；D16S539：12，13；D18S51：11，17；D19S433：14，16；D21S11：29，32.2；D2S1338：17，25；D3S1358：17；D5S818：13；D7S820：10，12；D8S1179：15；FGA：22；TH01：7，9.3；TPOX：8，11；vWA：18，19； Amelogenin：X，Y；CSF1PO：12；D13S317：8，11；D16S539：12，13；D18S51：11，17；D19S433：14，16；D21S11：29，32.2；D2S1338：17，25；D3S1358：17；D5S818：13；D7S820：10，12；D8S1179：15；FGA：22；TH01：7，9.3；TPOX：8，11；vWA：18，19； 3131C0001000700133 Human Colorectal Adenocarcinoma Cells; HCT-15 [HCT15] 人结直肠腺癌细胞；HCT-15 [HCT15] epithelial cell 上皮细胞 adherent growth 贴壁生长 DNA fingerprinting证据表明，这株细胞和DLD-1 (ATCC CCL-221) 来源于同一个人；但同工酶及细胞染色体组型分析仍存疑问。 细胞呈CSAp阴性(CSAp-)。 角蛋白免疫过氧化物酶染色阳性。 The evidence of DNA fingerprinting showes that this cell line is derived from the same person as DLD-1 (ATCC CCL-221), but the analysis of isozymes and cell karyotype remained questionable. The cells are negative in test for CSAp(CSAP-). It is positive in immunoperoxidase staining. RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 HCT-15 HCT-15 cell HCT-15 细胞 HCT15 HCT15 cell HCT15 细胞 NICR701 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR701 cell NICR701 细胞 http://www.cellresource.cn/fdetail.aspx?id=701 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：12；D13S317：8，11；D16S539：12，13；D18S51：11，17；D19S433：14，16；D21S11：29，32.2；D2S1338：17，25；D3S1358：17；D5S818：13；D7S820：10，11.3；D8S1179：15；FGA：22；TH01：7，9.3；TPOX：8，11；vWA：18，19； Amelogenin：X；CSF1PO：12；D13S317：8，11；D16S539：12，13；D18S51：11，17；D19S433：14，16；D21S11：29，32.2；D2S1338：17，25；D3S1358：17；D5S818：13；D7S820：10，11.3；D8S1179：15；FGA：22；TH01：7，9.3；TPOX：8，11；vWA：18，19； 3111C0001CCC000104 Human Colorectal Adenocarcinoma Cells; HCT-8 [HRT-18] 人结直肠腺癌细胞；HCT-8 [HRT-18] epithelioid 上皮样 adherent growth 贴壁生长 The cells is cytokeratin-positive, expressing CEA and alkaline phosphatase, and is the same cell with HRT-18. 该细胞角蛋白阳性；表达CEA、碱性磷酸酶；与HRT-18为同一细胞。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 81~90 81~90 A类 Class A HCT-8-Cell Morphology-PUMC HCT-8-Species Identification-PUMC HCT-8-物种鉴定-PUMC HCT-8-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代，2~3天换液1次。 Passage at 1: 4 ~ 1: 8, and change fluid once in 2~3 days. HCT-8 HCT-8 cell HCT-8 细胞 HRT-18 HRT-18 cell HRT-18 细胞 NICR104 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR104 cell NICR104 细胞 http://www.cellresource.cn/fdetail.aspx?id=104 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf HCT-8-Cell Morphology-PUMC HCT-8-Species Identification-PUMC HCT-8-物种鉴定-PUMC HCT-8-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:12；D13S317:8,11；D16S539:12,13；D18S51:11,17；D19S433:14,16；D21S11:29,32.2；D2S1338:17,25；D3S1358:17；D5S818:13；D7S820:10,11.3；D8S1179:15；FGA:22；TH01:7,9.3；TPOX:8,11；vWA:18,19 Amelogenin:X,Y；CSF1PO:12；D13S317:8,11；D16S539:12,13；D18S51:11,17；D19S433:14,16；D21S11:29,32.2；D2S1338:17,25；D3S1358:17；D5S818:13；D7S820:10,11.3；D8S1179:15；FGA:22；TH01:7,9.3；TPOX:8,11；vWA:18,19 3131C0001000700018 Human Colorectal Adenocarcinoma Cells; HCT-8 [HRT-18] 人结直肠腺癌细胞；HCT-8 [HRT-18] epithelial cell 上皮细胞 adherent growth 贴壁生长 HCT-8 is the same as HRT-18. It is positive in immunoperoxidase staining for keratin. HCT-8与HRT-18是一样的。 角蛋白免疫过氧化物酶染色阳性。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 HCT-8 HCT-8 cell HCT-8 细胞 HRT-18 HRT-18 cell HRT-18 细胞 NICR589 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR589 cell NICR589 细胞 http://www.cellresource.cn/fdetail.aspx?id=589 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；CSF1PO：11，12；D13S317：11，12；D16S539：11，12；D18S51：13；D19S433：14；D21S11：29，30；D2S1338：19，23；D3S1358：15，16.3；D5S818：11，12；D7S820：10；D8S1179：10，16；FGA：20，22；TH01：6，9；TPOX：8，9；vWA：17，19； Amelogenin：X；CSF1PO：11，12；D13S317：11，12；D16S539：11，12；D18S51：13；D19S433：14；D21S11：29，30；D2S1338：19，23；D3S1358：15，16.3；D5S818：11，12；D7S820：10；D8S1179：10，16；FGA：20，22；TH01：6，9；TPOX：8，9；vWA：17，19； 3111C0001CCC000109 Human Colorectal Adenocarcinoma Cells; HT-29 人结直肠腺癌细胞；HT-29 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from primary tumors in 1964 by Fogh J with transplantation culture and F12 culture medium containing 15% FBS. Recently, the cultured cells of the established strain were cultured in McCoy's 5a medium containing serum. This cell line is tumorigenic in nude mice as well as in steroid-treated hamsters. The cells can synthesize IgA, CEA, TGFβ binding proteins and myxins, expressing urokinase receptor, but no plasminogen activity is detected, and do not express CD4, while galactosylceramide is expressed on the cell surface (a possible replacement receptor for HIV). The oncogene tests for c-myc, K-ras, H-ras, N-ras, Myb, sis, fos are positive; The p53 gene is overexpressed and a G → A mutation occurs at codon 273, resulting in the replacement of Arg → His. 该细胞是1964年由Fogh J用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的。近来，已建株的培养细胞用含血清的McCoy＇s 5a培养基培养。 该细胞系在裸鼠中成瘤，也能在类固醇处理的地鼠中成瘤。该细胞可合成IgA、CEA、TGFβ结合蛋白和黏液素；表达尿激酶受体，但没有检测到血浆酶原活性；不表达CD4，但细胞表面表达半乳糖神经酰胺（HIV的可能替代受体）。该细胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos阳性；p53基因过表达，并且在273位密码子处发生G→A突变，导致Arg→His替换。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf P143 P143 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 64~69 64~69 A类 Class A HT-29-Cell Morphology-PUMC HT-29-Species Identification-PUMC HT-29-cell morphology-PUMC HT-29-物种鉴定-PUMC HT-29-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1：8传代；每周2~3次换液。 Passage at 1: 3 ~ 1: 8; and change fluid for 2 to 3 times per week. HT-29 HT-29 cell HT-29 细胞 NICR109 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR109 cell NICR109 细胞 http://www.cellresource.cn/fdetail.aspx?id=109 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf HT-29-Cell Morphology-PUMC HT-29-Species Identification-PUMC HT-29-cell morphology-PUMC HT-29-物种鉴定-PUMC HT-29-细胞形态-PUMC HT-29-细胞形态-PUMC Amelogenin:X；CSF1PO:11,12；D13S317:11,12；D16S539:11,12；D18S51:13；D19S433:14；D21S11:29,30；D2S1338:19,23；D3S1358:15,17；D5S818:11,12；D7S820:10；D8S1179:10,16；FGA:20,22；TH01:6,9；TPOX:8,9；vWA:17,19 Amelogenin:X；CSF1PO:11,12；D13S317:11,12；D16S539:11,12；D18S51:13；D19S433:14；D21S11:29,30；D2S1338:19,23；D3S1358:15,17；D5S818:11,12；D7S820:10；D8S1179:10,16；FGA:20,22；TH01:6,9；TPOX:8,9；vWA:17,19 3131C0001000700103 Human Colorectal Adenocarcinoma Cells; HT-29 人结直肠腺癌细胞；HT-29 epithelial cell 上皮细胞 adherent growth 贴壁生长 1964年，HT-29细胞系由J.Fogh 用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离。 近来，已建株的培养细胞用含相同血清McCoy＇s 5a培液培养。 该细胞系在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。 这个细胞株合成IgA, CEA, 蛋白绑定TGFβ的分泌成分和粘液素。 In 1964, HT-29 cell line was isolated from primary tumor through transplantation culture with and F12 culture medium containing 15% FBS by J. Fogh. Recently, established cells were cultured in McCoy's 5a culture liquid with the same serum. This cell line is tumorigenic in nude mice and can also lead to tumors in steroid -treated hamsters. The cell line synthesize secretory component and mucus in binding of IgA, CEA to TGF β. McCoy＇s 5A Media (Modified with Tricine) qualified fetal bovine serum，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P(128+5) P(128+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A HT-29-DNA-Fingerprinting-SHCRC HT-29-DNA-指纹-SHCRC HT-29-cell morphology-SHCRC HT-29-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 HT-29 HT-29 cell HT-29 细胞 NICR671 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR671 cell NICR671 细胞 http://www.cellresource.cn/fdetail.aspx?id=671 McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf HT-29-DNA-Fingerprinting-SHCRC HT-29-DNA-指纹-SHCRC HT-29-cell morphology-SHCRC HT-29-cell morphology-SHCRC HT-29-细胞形态-SHCRC HT-29-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000135 Human Colorectal Adenocarcinoma Cells; HT-29 人结直肠腺癌细胞；HT-29 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were isolated from primary tumors in 1964 by Fogh J with transplantation culture and F12 culture medium containing 15% FBS. Recently, the cultured cells of the established strain were cultured in McCoy's 5a medium containing serum. This cell line is tumorigenic in nude mice as well as in steroid-treated hamsters. The cells can synthesize IgA, CEA, TGFβ binding proteins and myxins, expressing urokinase receptor, but no plasminogen activity is detected, and do not express CD4, while galactosylceramide is expressed on the cell surface (a possible replacement receptor for HIV). The oncogene tests for c-myc, K-ras, H-ras, N-ras, Myb, sis, fos are positive; The p53 gene is overexpressed and a G → A mutation occurs at codon 273, resulting in the replacement of Arg → His. 该细胞是1964年由Fogh J用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的。近来，已建株的培养细胞用含血清的McCoy＇s 5a培养基培养。 该细胞系在裸鼠中成瘤，也能在类固醇处理的地鼠中成瘤。该细胞可合成IgA、CEA、TGFβ结合蛋白和黏液素；表达尿激酶受体，但没有检测到血浆酶原活性；不表达CD4，但细胞表面表达半乳糖神经酰胺（HIV的可能替代受体）。该细胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos阳性；p53基因过表达，并且在273位密码子处发生G→A突变，导致Arg→His替换。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C130 C130 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HT-29 HT-29 cell HT-29 细胞 NICR903 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR903 cell NICR903 细胞 http://www.cellresource.cn/fdetail.aspx?id=903 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；D8S1179：10, 16；D21S11：29,30；D7S820：10；CSF1PO：11, 12；D3S1358：15, 17；TH01：6, 9；D13S317：11, 12；D16S539：11, 12；D2S1338：19, 23；D19S433：14；vWA：17, 19；THOX：8, 9；D18S51：13；D5S818：11, 12；FGA：20, 22。 Amelogenin：X；D8S1179：10, 16；D21S11：29,30；D7S820：10；CSF1PO：11, 12；D3S1358：15, 17；TH01：6, 9；D13S317：11, 12；D16S539：11, 12；D2S1338：19, 23；D19S433：14；vWA：17, 19；THOX：8, 9；D18S51：13；D5S818：11, 12；FGA：20, 22。 3153C0001000000017 Human Colorectal Adenocarcinoma Cells; HT-29 人结直肠腺癌细胞；HT-29 epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 The cells were isolated from primary tumors in 1964 by Fogh J with transplantation culture and F12 culture medium containing 15% FBS. Recently, the cultured cells of the established strain were cultured in McCoy's 5a medium containing serum. This cell line is tumorigenic in nude mice as well as in steroid-treated hamsters. The cells can synthesize IgA, CEA, TGFβ binding proteins and myxins, expressing urokinase receptor, but no plasminogen activity is detected, and do not express CD4, while galactosylceramide is expressed on the cell surface (a possible replacement receptor for HIV). The oncogene tests for c-myc, K-ras, H-ras, N-ras, Myb, sis, fos are positive; The p53 gene is overexpressed and a G → A mutation occurs at codon 273, resulting in the replacement of Arg → His. 该细胞是1964年由Fogh J用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的。近来，已建株的培养细胞用含血清的McCoy＇s 5a培养基培养。 该细胞系在裸鼠中成瘤，也能在类固醇处理的地鼠中成瘤。该细胞可合成IgA、CEA、TGFβ结合蛋白和黏液素；表达尿激酶受体，但没有检测到血浆酶原活性；不表达CD4，但细胞表面表达半乳糖神经酰胺（HIV的可能替代受体）。该细胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos阳性；p53基因过表达，并且在273位密码子处发生G→A突变，导致Arg→His替换。 RPMI 1640 (w/o Hepes) 15%NBS RPMI 1640 (w/o Hepes) 15%NBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） 68-72 68-72 A类 Class A HT-29-cell morphology-KCB HT-29-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1：3传代；3-4天一次 Passage at 1: 3; and passage once in 3-4 days HT-29 HT-29 cell HT-29 细胞 NICR1291 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1291 cell NICR1291 细胞 http://www.cellresource.cn/fdetail.aspx?id=1291 RPMI 1640 (w/o Hepes) 15%NBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 15%NBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf HT-29-cell morphology-KCB HT-29-细胞形态-KCB Amelogenin：X，Y；CSF1PO：10，11，13，14；D13S317：8，11；D16S539：9，12；D18S51：13，18；D19S433：14，15；D21S11：29，31.2；D2S1338：17，18；D3S1358：14，OL；D5S818：11，13；D7S820：10，11；D8S1179：10；FGA：18，20；TH01：9.3；TPOX：8，9；vWA：17，18； Amelogenin：X，Y；CSF1PO：10，11，13，14；D13S317：8，11；D16S539：9，12；D18S51：13，18；D19S433：14，15；D21S11：29，31.2；D2S1338：17，18；D3S1358：14，OL；D5S818：11，13；D7S820：10，11；D8S1179：10；FGA：18，20；TH01：9.3；TPOX：8，9；vWA：17，18； 3111C0001CCC000164 Human Colorectal Adenocarcinoma Cells; LoVo 人结直肠腺癌细胞；LoVo epithelioid 上皮样 adherent growth 贴壁生长 The cells were constructed in 1971 from the a metastasis of the left supraclavicular region of a 56-year-old white male diagnosed as colon adenocarcinoma. The expression of oncogene c-myc, K-ras, H-ras, N-ras, Myb and fos was positive, but the expression of sis and N-myc was not detected. It can form tumors in nude mice and express tumor-specific nuclear matrix protein CC-3 and CC-4. 该细胞是1971年从诊断为结肠腺癌的56岁白人男性的一个左锁骨上区的转移灶建系的。 癌基因c-myc、K-ras、H-ras、N-ras、Myb和fos的表达呈阳性；未检测到sis和N-myc的表达。它在裸鼠中能成瘤；表达肿瘤特异的核基质蛋白蛋白CC-3和CC-4。 F12K 10%FBS F12K 10%FBS http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 44~46,XY 44~46,XY A类 Class A LoVo-Cell Morphology-PUMC LoVo-Species Identification-PUMC LoVo-物种鉴定-PUMC LoVo-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:10传代；2~3天换液1次。 Passage at 1: 3 ~ 1:10; and change fluid once in 2~3 days. LoVo LoVo cell LoVo 细胞 NICR164 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR164 cell NICR164 细胞 http://www.cellresource.cn/fdetail.aspx?id=164 F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf LoVo-Cell Morphology-PUMC LoVo-Species Identification-PUMC LoVo-物种鉴定-PUMC LoVo-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:10,11,13,14；D13S317:8,10,11；D16S539:9,12；D18S51:13,18；D19S433:14,15；D21S11:29,31.3；D2S1338:17,18；D3S1358:14,16,17,18；D5S818:11,13；D7S820:9.3,10,11；D8S1179:10；FGA:18,20；TH01:9.3；TPOX:8,9；vWA:17,18 Amelogenin:X,Y；CSF1PO:10,11,13,14；D13S317:8,10,11；D16S539:9,12；D18S51:13,18；D19S433:14,15；D21S11:29,31.3；D2S1338:17,18；D3S1358:14,16,17,18；D5S818:11,13；D7S820:9.3,10,11；D8S1179:10；FGA:18,20；TH01:9.3；TPOX:8,9；vWA:17,18 3131C0001000700082 Human Colorectal Adenocarcinoma Cells; LoVo 人结直肠腺癌细胞；LoVo epithelial cell 上皮细胞 adherent growth 贴壁生长 LoVo was established in 1971, from a tumor nodule moved to the left supraclavicular region of a 56-year-old male white man diagnosed with colon adenocarcinoma. The expression of oncogene C-myc, K-ras, H-ras, N-ras, Myb, sis and fos is positive. The expression of oncogene N-myc is not detected. It can form a tumor in nude mice. Five nude mice were inoculated subcutaneously together with 107 cells and form tumor after 21 days. The cells express of tumor-specific nuclear matrix protein CC-3 and CC-4. LoVo建自1971年，从诊断为结肠腺癌的56岁男性白人的一个转移到左锁骨上区的肿瘤结节建系。 癌基因Ｃ-myc, K-ras, H-ras, N-ras, Myb, sis 和fos的表达呈阳性。 癌基因N-myc的表达未做检测。 它在裸鼠中能成瘤。 与107细胞联合皮下接种５只裸鼠21天后全部成瘤。 表达肿瘤特异的核基质蛋白蛋白CC-3和CC-4。 F12K qualified fetal bovine serum，10% F12K 优质胎牛血清，10% F12K 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf P(29+5) P(29+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Lovo-cell morphology-SHCRC Lovo-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 LoVo LoVo cell LoVo 细胞 NICR650 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR650 cell NICR650 细胞 http://www.cellresource.cn/fdetail.aspx?id=650 F12K 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf Lovo-cell morphology-SHCRC Lovo-细胞形态-SHCRC 3142C0001000000129 Human Colorectal Adenocarcinoma Cells; LS 人结直肠腺癌细胞；LS epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell is a trypsin variant of LS 180 colon adenocarcinoma cell line. It is more transmissible than its parents and produces a large amount of carcinoembryonic antigen (CEA) like LS 180. Electron microscopy showed that it had abundant microfilament and cytoplasm mucin vacuoles; serological detection for rectal antigen 3 was positive; p53 antigen expression was negative, but p53 mRNA expression was positive; the cells were cytokeratin-positive; expression for oncogene c-myc, N-myc, H-ras, N-ras, Myb and fos was positive; the expression of oncogene k-ras and sis was not detected; the cells can express IL-10, IL-6 and mucin. 该细胞是LS 180结肠腺癌细胞株的胰蛋白酶化变种。它比亲本更易传代，像LS 180一样生成大量的癌胚抗原(CEA)。电镜研究表明，它有丰富的微丝和胞浆黏液素液泡；血清学检测直肠抗原3阳性；p53抗原表达阴性，但mRNA表达阳性；角蛋白阳性；癌基因c-myc、N-myc、H-ras、N-ras、Myb和fos的表达呈阳性；癌基因k-ras和sis的表达未做检测；可产生IL-10、IL-6和黏蛋白。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C104 C104 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. LS LS cell LS 细胞 NICR897 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR897 cell NICR897 细胞 http://www.cellresource.cn/fdetail.aspx?id=897 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：11；D13S317：8，11；D16S539：11，12；D18S51：15，16；D19S433：15，18.2；D21S11：32.2；D2S1338：14，16；D3S1358：D5S818：11.1；D7S820：10，11；D8S1179：12.2，14，15；FGA：20，22；TH01：6，9.3；TPOX：8，11；vWA：16； Amelogenin：X；CSF1PO：11；D13S317：8，11；D16S539：11，12；D18S51：15，16；D19S433：15，18.2；D21S11：32.2；D2S1338：14，16；D3S1358：D5S818：11.1；D7S820：10，11；D8S1179：12.2，14，15；FGA：20，22；TH01：6，9.3；TPOX：8，11；vWA：16； 3111C0001CCC000359 Human Colorectal Adenocarcinoma Cells; NCI-H716 人结直肠腺癌细胞；NCI-H716 epithelial-like 上皮细胞样 suspension growth, in clusters, A few adherent 悬浮生长，聚团，少数贴壁 This cell line was established in 1984 and originated from ascites in a 33-year-old male with colorectal adenocarcinoma after 5-fu treatment. 该细胞1984年建系，源自一位33岁患有大肠腺癌男性经5-fu治疗后的腹水。 RPMI 1640 (w/o Hepes) 10% FBS RPMI 1640 (w/o Hepes) 10% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P35 P35 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 55～64 55～64 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3～1:6传代，每周换液2～3次 Passage at 1: 3 ~ 1: 6, and change fluid for 2 to 3 times a week NCI-H716 NCI-H716 cell NCI-H716 细胞 NICR1548 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1548 cell NICR1548 细胞 http://www.cellresource.cn/fdetail.aspx?id=1548 RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001001200002 Human Colorectal Adenocarcinoma Cells; NCI-H716 人结直肠腺癌细胞；NCI-H716 epithelial-like 上皮细胞样 suspension growth, in clusters, A few adherent 悬浮生长，聚团，少数贴壁 This cell line was established in 1984 and originated from ascites in a 33-year-old male with colorectal adenocarcinoma after 5-fu treatment. 该细胞1984年建系，源自一位33岁患有大肠腺癌男性经5-fu治疗后的腹水。 RPMI 1640 (w/o Hepes) 10% FBS RPMI 1640 (w/o Hepes) 10% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B NCI-H716-cell morphology-SHCRC NCI-H716-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:3～1:6传代，每周换液2～3次 Passage at 1: 3 ~ 1: 6, and change fluid for 2 to 3 times a week NCI-H716 NCI-H716 cell NCI-H716 细胞 NICR1583 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1583 cell NICR1583 细胞 http://www.cellresource.cn/fdetail.aspx?id=1583 RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H716-cell morphology-SHCRC NCI-H716-细胞形态-SHCRC 3131C0001000700210 Human Colorectal Adenocarcinoma Cells; NCI-H716 [H716] 人结直肠腺癌细胞；NCI-H716 [H716] epithelial cell 上皮细胞 adherent growth 贴壁生长 The cell line was established from cells obtained from ascites of a patient treated with 5-fluorouracil. Unlike other colorectal cancer cell lines, this cell line has dopa decarboxylase and has dense core endocrine granules in the cytoplasm. This cell line does not express TAG-72 or CA19-9 antigen and does not produce carcinoembryonic antigen (CEA). 从一位经5-氟尿嘧啶治疗的患者腹水中得到的细胞建立了这个细胞株。 与其它结直肠癌细胞系不同，这株细胞有多巴脱羧酶，细胞质中有核心致密的内分泌型颗粒。 这株细胞不表达TAG-72 或CA19-9抗原，也不生成癌胚抗原（CEA）。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A NCI-H716-cell morphology-SHCRC NCI-H716-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 H716 H716 cell H716 细胞 NCI-H716 NCI-H716 cell NCI-H716 细胞 NICR1714 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1714 cell NICR1714 细胞 http://www.cellresource.cn/fdetail.aspx?id=1714 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf NCI-H716-cell morphology-SHCRC NCI-H716-细胞形态-SHCRC 3131C0001001200006 Human Colorectal Adenocarcinoma Cells; SK-CO-1 人结直肠腺癌细胞；SK-CO-1 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from metastatic ascites in colorectal patients. 该细胞来源于结直肠病人的转移性腹水。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2~1:3传代，每周2~3次。 Passage at 1: 2 ~ 1: 3, and passage for 2 to 3 times per week. NICR1587 SK-CO-1 SK-CO-1 cell SK-CO-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1587 cell NICR1587 细胞 http://www.cellresource.cn/fdetail.aspx?id=1587 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin：X，Y；CSF1PO：11，13，15；D13S317：10；D16S539：10；D18S51：13；D19S433：14；D21S11：27，28，31；D2S1338：23，24；D3S1358：14，15；D5S818：11，12，13；D7S820：10，11，13；D8S1179：14，15；FGA：19，20，22.2；TH01：6，7；TPOX：8；vWA：17，20 Amelogenin：X，Y；CSF1PO：11，13，15；D13S317：10；D16S539：10；D18S51：13；D19S433：14；D21S11：27，28，31；D2S1338：23，24；D3S1358：14，15；D5S818：11，12，13；D7S820：10，11，13；D8S1179：14，15；FGA：19，20，22.2；TH01：6，7；TPOX：8；vWA：17，20 3111C0001CCC000206 Immortalized Human Lens Epithelial Cell Line; SRA01/04（HLE） 人晶体上皮细胞永生系；SRA01/04（HLE） epithelioid 上皮样 adherent growth 贴壁生长 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） B类 Class B Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；3~4天1次。 Passage at 1: 3; and passage once in 3 to 4 days. NICR206 SRA01/04（HLE） SRA01/04（HLE） cell SRA01/04（HLE） 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR206 cell NICR206 细胞 http://www.cellresource.cn/fdetail.aspx?id=206 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf AMEL:X,X；CSF1PO:13,13；D12S391:19,20；D13S317:13,13；D16S539:11,12；D18S51:14,14；D19S433:15,15；D21S11:31,31；D2S1338:20,24；D2S441:10,14；D3S1358:16,16；D5S818:11,11；D6S1043:11,11；D7S820:11,12；D8S1179:12,14；FGA:20,20；Penta D:9,9；Penta E:10,13；TH01:6,9.3；TPOX:11,1 AMEL:X,X；CSF1PO:13,13；D12S391:19,20；D13S317:13,13；D16S539:11,12；D18S51:14,14；D19S433:15,15；D21S11:31,31；D2S1338:20,24；D2S441:10,14；D3S1358:16,16；D5S818:11,11；D6S1043:11,11；D7S820:11,12；D8S1179:12,14；FGA:20,20；Penta D:9,9；Penta E:10,13；TH01:6,9.3；TPOX:11,1 3142C0001000000955 Human Osteogenic Sarcoma Cell; U-2 OS 人胫骨肉瘤细胞；U-2 OS epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were isolated from the tibia of a 15-year-old female sarcoma patient in 1964 and were suitable for transfection host, 该细胞是1964年从一位15岁女性肉瘤患者的胫骨中分离出来的，适合做转染宿主 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR2192 U-2 OS U-2 OS cell U-2 OS 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2192 cell NICR2192 细胞 http://www.cellresource.cn/fdetail.aspx?id=2192 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf Amelogenin：X；CSF1PO：9，10；D13S317：10，11；D16S539：11；D18S51：15，20；D19S433：14，15；D21S11：30，32.2；D2S1338：19，25；D3S1358：16，19；D5S818：11；D7S820：11；D8S1179：13，16；FGA：18，22；TH01：6，8；TPOX：8；vWA：16，18； Amelogenin：X；CSF1PO：9，10；D13S317：10，11；D16S539：11；D18S51：15，20；D19S433：14，15；D21S11：30，32.2；D2S1338：19，25；D3S1358：16，19；D5S818：11；D7S820：11；D8S1179：13，16；FGA：18，22；TH01：6，8；TPOX：8；vWA：16，18； 3111C0001CCC000112 Human Megakaryoblastic Leukemia Cell Line; Mo7e 人巨细胞白血病细胞株；Mo7e round 圆形 semi-adherent growth 半贴壁生长 This cell line was established in 1987 and originated from the peripheral blood of 6-month-old girl with acute megakaryocytic leukemia (AML M7). It is a subline of M-07 cell line; GM-CSF, IFN-α, IFN-β, IFN-γ, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-α, TPO can promote cell proliferation. The cells can grow without dependence on cytokine, but growing slowly. It can be used to determine the activity of various cytokines. 该细胞系1987年建系，源于6个月龄患有急性巨核细胞白血病（AML M7）女婴的外周血，是M-07细胞系的亚系， GM-CSF、IFN-α、IFN-β、IFN-γ、IL-2、IL-3、IL-4、IL-6、IL-15、NGF、SCF、TNF-α、TPO可促进细胞增殖。该细胞也可不依赖细胞因子生长，但生长缓慢。可用于测定多种细胞因子的活性。 RPMI 1640 (w/o Hepes) 10%FBS；8ng/ml rHuGM-csf；10%CO2 RPMI 1640 (w/o Hepes) 10%FBS；8ng/ml rHuGM-csf；10%CO2 http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 43~46,XX,Xq-，12q+ 43~46,XX,Xq-，12q+ A类 Class A Mo7e-Species Identification-PUMC Mo7e-cell morphology-PUMC Mo7e-物种鉴定-PUMC Mo7e-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 0.5 ~ 1.0 � 106 cells/ml, and passage at 1: 2 ~ 1: 3, changing fluid every 2 days. 维持细胞浓度在 0.5~1.0×106 cells/ml，1:2~1:3传代，每2天换液1次。 Mo7e Mo7e cell Mo7e 细胞 NICR112 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR112 cell NICR112 细胞 http://www.cellresource.cn/fdetail.aspx?id=112 RPMI 1640 (w/o Hepes) 10%FBS；8ng/ml rHuGM-csf；10%CO2 URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；8ng/ml rHuGM-csf；10%CO2 URI: http://www.cellresource.cn/pdf/20080108101040.pdf Mo7e-Species Identification-PUMC Mo7e-cell morphology-PUMC Mo7e-物种鉴定-PUMC Mo7e-细胞形态-PUMC Amelogenin：X；CSF1PO：9，10；D13S317：12，14；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：21；TH01：7；TPOX：8，12；vWA：16，18； Amelogenin：X；CSF1PO：9，10；D13S317：12，14；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：21；TH01：7；TPOX：8，12；vWA：16，18； 3111C0001CCC000163 Human Oral Epidermoid Carcinoma Cells; KB 人口腔表皮样癌细胞；KB epithelioid 上皮样 adherent growth 贴壁生长 The cell was initially thought to originate from oral epithelial carcinoma, but subsequent analysis of isozyme analysis, HeLa-labeled chromosomes and DNA fingerprinting found that the origin cell was contaminated with HeLa. The cells were keratin-positive. KB cells are reported to contain the human papillomavirus 18 (HPV-18) sequence. 最初认为这个细胞源自口腔表皮癌，但随后通过同工酶分析、HeLa标记染色体和DNA指纹分析发现，起源细胞已被HeLa污染。角蛋白阳性。有报告称，KB细胞含有人乳头状瘤病毒18 (HPV-18)序列。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf P368 P368 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 72~82 72~82 A类 Class A KB-Species Identification-PUMC KB-cell morphology-PUMC KB-物种鉴定-PUMC KB-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:10传代；2~3天换液1次。 Passage at 1: 4 ~ 1:10; and change fluid once in 2~3 days. KB KB cell KB 细胞 NICR163 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR163 cell NICR163 细胞 http://www.cellresource.cn/fdetail.aspx?id=163 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100822.pdf KB-Species Identification-PUMC KB-cell morphology-PUMC KB-物种鉴定-PUMC KB-细胞形态-PUMC Amelogenin:X；CSF1PO:9,10；D13S317:12,14；D16S539:9,10；D18S51:16；D19S433:13；D21S11:27,28；D2S1338:17；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:21；TH01:7；TPOX:8,12；vWA:16,18 Amelogenin:X；CSF1PO:9,10；D13S317:12,14；D16S539:9,10；D18S51:16；D19S433:13；D21S11:27,28；D2S1338:17；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:21；TH01:7；TPOX:8,12；vWA:16,18 3131C0001000700073 Human Oral Epidermoid Carcinoma Cells; KB 人口腔表皮样癌细胞；KB epithelial cell 上皮细胞 adherent growth 贴壁生长 The cell was initially thought to originate from oral epithelial carcinoma, but subsequent analysis of isozyme analysis, HeLa-labeled chromosomes and DNA fingerprinting found that the origin cell was contaminated with HeLa. The cells are positive for keratin by immunoperoxidase staining. KB cells are reported to contain the human papillomavirus 18 (HPV-18) sequence. 最初认为这个细胞源自口腔表皮癌，但随后通过同工酶分析、HeLa标记染色体和DNA指纹分析发现，起源细胞已被HeLa污染。 角蛋白免疫过氧化物酶染色阳性。 有报告称KB细胞含有人乳头状瘤病毒18 (HPV-18)序列。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) fetal bovine serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A KB-DNA-Fingerprinting-SHCRC KB-DNA-指纹-SHCRC KB-cell morpholgoy-SHCRC KB-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen Unit: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences Zip code: 200032 Address: No. 320, Yueyang Road, Shanghai Tel: 021-54920404，021-54920405 Fax: 021-54920560 Email: shchen@sibs.ac.cn 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920560 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 KB KB cell KB 细胞 NICR641 1.Eagle H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med, 1955，89: 362-364. 2.Boshart M, Gissmann L, Ikenberg H, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J, 1984，3: 1151-1157. 1.Eagle H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med, 1955，89: 362-364. 2.Boshart M, Gissmann L, Ikenberg H, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J, 1984，3: 1151-1157. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR641 cell NICR641 细胞 http://www.cellresource.cn/fdetail.aspx?id=641 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf KB-DNA-Fingerprinting-SHCRC KB-DNA-指纹-SHCRC KB-cell morpholgoy-SHCRC KB-细胞形态-SHCRC 3142C0001000000512 Human T-cell Hybridoma Cells; 31E9 人类T细胞杂交瘤细胞；31E9 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cell is patented and its characteristics have not been disclosed. 该细胞属专利保藏，其特征特性尚未公开。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C7 C7 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Undefined 未定 Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代，3-4天传1次 Passage at 1: 3, and passage once in 3-4 days. 31E9 31E9 cell 31E9 细胞 NICR1159 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1159 cell NICR1159 细胞 http://www.cellresource.cn/fdetail.aspx?id=1159 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X，Y；CSF1PO：12；D13S317：8；D16S539：10，12；D18S51：14，17；D19S433：14，14.2；D21S11：31.2；D2S1338：18，23；D3S1358：16；D5S818：12；D7S820：8；D8S1179：11，15；FGA：23；TH01：6，9；TPOX：10；vWA：14，18，19； Amelogenin：X，Y；CSF1PO：12；D13S317：8；D16S539：10，12；D18S51：14，17；D19S433：14，14.2；D21S11：31.2；D2S1338：18，23；D3S1358：16；D5S818：12；D7S820：8；D8S1179：11，15；FGA：23；TH01：6，9；TPOX：10；vWA：14，18，19； 3111C0001CCC000120 Human Megakaryocytic Leukemic Cells; UT-7 人类原巨核细胞型白血病细胞；UT-7 round 圆形 suspension growth, 1%-2% of the cells in slight adherent 悬浮生长，有1%~2%的细胞可轻微贴壁。 The cells were established in 1988 and derived from the bone marrow of a 64-year-old male with acute myeloid leukemia (AML M7), and responded to a variety of cytokines. 该细胞于1988年建系；源于一名64岁患有急性粒细胞白血病（AML M7）的男性的骨髓；对多种细胞因子有反应。 RPMI 1640 (w/o Hepes) 10%FBS；5-7u/ml Epo RPMI 1640 (w/o Hepes) 10%FBS；5-7u/ml Epo http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C7 C7 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A UT-7-Species Identification-PUMC UT-7-cell morphology-PUMC UT-7-物种鉴定-PUMC UT-7-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 1.0 ~ 1.5 � 106 cells/ml, passage at 1: 2, and passage once in 2 to 3 days. 维持细胞浓度在1.0~1.5×106 cells/ml，1：2传代，2~3天1次。 NICR120 UT-7 UT-7 cell UT-7 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR120 cell NICR120 细胞 http://www.cellresource.cn/fdetail.aspx?id=120 RPMI 1640 (w/o Hepes) 10%FBS；5-7u/ml Epo URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；5-7u/ml Epo URI: http://www.cellresource.cn/pdf/20080108101040.pdf UT-7-Species Identification-PUMC UT-7-cell morphology-PUMC UT-7-物种鉴定-PUMC UT-7-细胞形态-PUMC Amelogenin：X；CSF1PO:10,12；D12S391:18,21；D13S317:12；D16S539:12；D18S51:14,18；D19S433:14,16；D21S11:29；D2S1338:16,19；D3S1358:16,18；D5S818:13；D6S1043:15,20；D7S820:9,11；D8S1179:10,15；FGA:22,26；Penta E:11,18；TH01:9.3；TPOX:8；vWA:14,16； Amelogenin：X；CSF1PO:10,12；D12S391:18,21；D13S317:12；D16S539:12；D18S51:14,18；D19S433:14,16；D21S11:29；D2S1338:16,19；D3S1358:16,18；D5S818:13；D6S1043:15,20；D7S820:9,11；D8S1179:10,15；FGA:22,26；Penta E:11,18；TH01:9.3；TPOX:8；vWA:14,16； 3111C0001CCC000382 Human Lymphoblastoid Cells (EBV Transformation); NCI-BL2009 人淋巴母细胞（EBV转化）；NCI-BL2009 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells are derived from peripheral blood B cells in a 68-year-old female patient with lung adenocarcinoma. 该细胞源自68岁肺腺癌女性患者的外周血B细胞。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell density in the 1 � 106 ~ 3 � 106 cells/ml, changing fluid for 2 to 3 times a week 保持细胞密度在 1×106～3×106 cells/ml之间，每周换液2～3次 NCI-BL2009 NCI-BL2009 cell NCI-BL2009 细胞 NICR1571 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1571 cell NICR1571 细胞 http://www.cellresource.cn/fdetail.aspx?id=1571 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3131C0001001100004 Human Lymphocytes; 1301 人淋巴细胞；1301 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Unknown Unknown Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1301 1301 cell 1301 细胞 NICR766 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR766 cell NICR766 细胞 http://www.cellresource.cn/fdetail.aspx?id=766 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf 3111C0002000000075 Human Ovarian Cancer Cells; A2780 人卵巢癌细胞；A2780 adherent growth 贴壁生长 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） B类 Class B National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net A2780 A2780 cell A2780 细胞 NICR447 NICR447 cell NICR447 细胞 http://www.cellresource.cn/fdetail.aspx?id=447 Amelogenin：X；CSF1PO：10，13；D13S317：12；D16S539：9；D18S51：18；D19S433：14，17；D21S11：30；D2S1338：16，17；D3S1358：16；D5S818：12；D7S820：10；D8S1179：9，14；FGA：24；TH01：7；TPOX：8，10；vWA：16，18； Amelogenin：X；CSF1PO：10，13；D13S317：12；D16S539：9；D18S51：18；D19S433：14，17；D21S11：30；D2S1338：16，17；D3S1358：16；D5S818：12；D7S820：10；D8S1179：9，14；FGA：24；TH01：7；TPOX：8，10；vWA：16，18； 3111C0001CCC000339 Human Ovarian Cancer Cells; Caov-3 人卵巢癌细胞；Caov-3 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cell line was established in 1976 and originated from ovarian adenocarcinoma tissue of a 54-year-old white woman. 该细胞1976年建系，源自一位54岁白人女性的卵巢腺癌组织。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P42 P42 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3～1:8传代，2～3天换液一次 Passage at 1: 3 ~ 1: 8, change fluid once in 2~3 days Caov-3 Caov-3 cell Caov-3 细胞 NICR1528 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1528 cell NICR1528 细胞 http://www.cellresource.cn/fdetail.aspx?id=1528 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：10；D13S317：12，14；D16S539：9，11；D18S51：21；D19S433：11，12；D21S11：28，33.2；D2S1338：17，20；D3S1358：16；D5S818：11；D7S820：8，10；D8S1179：12；FGA：22；TH01：6，9.3；TPOX：8，9；vWA：16，17； Amelogenin：X；CSF1PO：10；D13S317：12，14；D16S539：9，11；D18S51：21；D19S433：11，12；D21S11：28，33.2；D2S1338：17，20；D3S1358：16；D5S818：11；D7S820：8，10；D8S1179：12；FGA：22；TH01：6，9.3；TPOX：8，9；vWA：16，17； 3111C0001CCC000367 Human Ovarian Cancer Cells; Caov-4 人卵巢癌细胞；Caov-4 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells originates from the tubal subserosa of a 45-year-old woman with ovarian adenocarcinoma. 该细胞源自一位45岁卵巢腺癌女性患者的浆膜下的输卵管。 L15: Leibovitz Medium 20%FBS L15: Leibovitz Medium 20%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P39 P39 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） Hypotriploid, Hypertetraploid 亚三倍体，超四倍体 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:3传代，每周换液2～3次 Passage at 1: 2 ~ 1: 3, and change fluid for 2～3 times a week Caov-4 Caov-4 cell Caov-4 细胞 NICR1556 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1556 cell NICR1556 细胞 http://www.cellresource.cn/fdetail.aspx?id=1556 L15: Leibovitz Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf STR Identification STR鉴定 3142C0001000000348 Human Ovarian Cancer Cells; ES-2 人卵巢癌细胞；ES-2 epithelial-like 上皮细胞样 adherent growth 贴壁生长 ES-2细胞系源于一位47岁黑人女性的临床卵巢透明细胞癌手术标本。该细胞对中低剂量的阿霉素，顺氯氨铂，双氯乙基亚硝脲，表鬼臼毒素吡喃葡糖苷等化疗药物有一定耐药性。该细胞少量表达糖蛋白P。 The ES-2 cell line was derived from surgical specimen of clinical ovarian clear cell carcinoma of a 47-year-old black female. The cells are resistant to moderate and low doses of adriamycin, cisplatin, bischloroethyl nitrosourea, teniposide and other chemotherapeutic drugs to a certain extent. The cells express low levels of P-glycoprotein. McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C10 C10 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A ES-2-Myoplasma Detection-CCTCC ES-2-cell morphology-CCTCC ES-2-支原体检测-CCTCC ES-2-细胞形态-CCTCC Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. ES-2 ES-2 cell ES-2 细胞 NICR1999 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1999 cell NICR1999 细胞 http://www.cellresource.cn/fdetail.aspx?id=1999 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf ES-2-Myoplasma Detection-CCTCC ES-2-cell morphology-CCTCC ES-2-支原体检测-CCTCC ES-2-细胞形态-CCTCC Amelogenin：X；CSF1PO：9，12；D13S317：9，10；D16S539：9，12；D18S51：15，18；D19S433：13；D21S11：29，31.2；D2S1338：24；D3S1358：15；D5S818：11；D7S820：9；D8S1179：14，15；FGA：24；TH01：7，9；TPOX：11；vWA：15，17； Amelogenin：X；CSF1PO：9，12；D13S317：9，10；D16S539：9，12；D18S51：15，18；D19S433：13；D21S11：29，31.2；D2S1338：24；D3S1358：15；D5S818：11；D7S820：9；D8S1179：14，15；FGA：24；TH01：7，9；TPOX：11；vWA：15，17； 3111C0001CCC000021 Human Ovarian Teratoma Cells; PA-1 人卵巢畸胎瘤细胞；PA-1 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from the peritoneal effusion of a 12-year-old white female patient with ovarian teratoma; the cells can form tightly woven clones, which can be differentiated in low-serum culture, low-density inoculation or with 5-bromo-2'-deoxyuridine treatment. This cell expressed the PCC4 embryonic antigen, but no F9 expression was detected. 该细胞是从一名12岁的白人女性卵巢畸胎瘤患者的腹腔积液中分离建立的；该细胞可以形成紧密的编织状的克隆，在低血清培养、低密度接种或用5-bromo-2＇-deoxyuridine处理时可以分化为胚状体。该细胞表达胚胎抗原PCC4，但未检测到F9的表达。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 40~46，XX 40~46，XX A类 Class A PA-1-cell morphology-PUMC PA-1-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:10传代；每周2~3次。　 Passage at 1: 4 ~ 1:10; and passage for 2 to 3 times a week. NICR21 PA-1 PA-1 cell PA-1 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR21 cell NICR21 细胞 http://www.cellresource.cn/fdetail.aspx?id=21 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf PA-1-cell morphology-PUMC PA-1-细胞形态-PUMC 3131C0001000700111 Human Ovarian Clear Cell Carcinoma Cells; ES-2 人卵巢透明细胞癌细胞；ES-2 fibroblasts 成纤维细胞 adherent growth 贴壁生长 ES-2细胞系建自45岁女性黑人的外科肿瘤标本。 该肿瘤为低分化卵巢透明细胞癌。 该细胞最初生长在软琼脂中。 在裸鼠中成瘤。 该细胞对多种化疗药物包括doxorubicin, cisplatin, carmustine, etoposide 和cyanomorpholinodoxorubicin (MRA-CN)表现出低到中等的耐受性。 ES-2细胞表达低水平的P糖蛋白。 The ES-2 cell line was established from a surgical tumor specimen from a 45-year-old black female. The tumor is a poorly differentiated ovarian clear cell carcinoma. The cells were initially grown in soft agar. The cells can form tumors in the nude mice. The cells showed a tolerance to a variety of chemotherapeutic drugs including dotorubicin, Cisplatin, carmustine, etoposide and cyclosporin (MRA-CN) from low to medium. ES-2 cells express low levels of P-glycoprotein. McCoy＇s 5A Media (Modified with Tricine) qualified fetal bovine serum，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P(63+5) P(63+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 ES-2 ES-2 cell ES-2 细胞 NICR679 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR679 cell NICR679 细胞 http://www.cellresource.cn/fdetail.aspx?id=679 McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf Amelogenin：X；CSF1PO：12；D13S317：10，12；D16S539：12；D18S51：13，14；D19S433：14，16；D21S11：27，29；D2S1338：17，20；D3S1358：16；D5S818：12；D7S820：9，11；D8S1179：12，13；FGA：22，25；TH01：6，9.3；TPOX：8，11；vWA：14，15； Amelogenin：X；CSF1PO：12；D13S317：10，12；D16S539：12；D18S51：13，14；D19S433：14，16；D21S11：27，29；D2S1338：17，20；D3S1358：16；D5S818：12；D7S820：9，11；D8S1179：12，13；FGA：22，25；TH01：6，9.3；TPOX：8，11；vWA：14，15； 3111C0001CCC000371 Human Ovarian Clear Cell Carcinoma Cells; ES-2 人卵巢透明细胞癌细胞；ES-2 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 The cells are derived from surgical tumor specimens of a 47-year-old black woman with clear cell carcinoma of the ovary and express low P-glycoprotein. 该细胞源自一位47岁患有卵巢透明细胞癌的黑人女性的外科手术肿瘤标本，低表达P糖蛋白。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P70 P70 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 66～88，XX 66～88，XX A类 Class A ES-2-Cell Morphology-PUMC ES-2-Species Identification-CRC ES-2-cell morphology-CRC ES-2-物种鉴定-CRC ES-2-细胞形态-CRC ES-2-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4～1:8传代，每周换液2～3次 Passage at 1: 4 ~ 1: 8, and change fluid for 2 to 3 times a week ES-2 ES-2 cell ES-2 细胞 NICR1560 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1560 cell NICR1560 细胞 http://www.cellresource.cn/fdetail.aspx?id=1560 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf ES-2-Cell Morphology-PUMC ES-2-Species Identification-CRC ES-2-cell morphology-CRC ES-2-物种鉴定-CRC ES-2-细胞形态-CRC ES-2-细胞形态-PUMC 3131C0001000700108 Human Ovarian Adenocarcinoma Cells; OVCAR-3 人卵巢腺癌细胞；OVCAR-3 adherent growth 贴壁生长 The cells were established in 1982 by T.C. Hamilton et al., derived from an ascites of a 60-year-old patient with ovarian adenocarcinoma, and is a model for the study of drug resistance in ovarian cancer. 该细胞1982年由T.C. Hamilton等建系，源自一位60卵巢腺癌的腹水，是卵巢癌抗药性研究的模型。 RPMI 1640 (w/o Hepes) 20% FBS RPMI 1640 (w/o Hepes) 20% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(19+5) P(19+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B OVCAR-3-cell morphology-SHCRC OVCAR-3-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2～1:4传代，每周换液2～3次 Passage at 1: 2 ~ 1: 4, and change fluid for 2 to 3 times a week NICR676 OVCAR-3 OVCAR-3 cell OVCAR-3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR676 cell NICR676 细胞 http://www.cellresource.cn/fdetail.aspx?id=676 RPMI 1640 (w/o Hepes) 20% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 20% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf OVCAR-3-cell morphology-SHCRC OVCAR-3-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000122 Human Ovarian Adenocarcinoma Cells; OVCAR-3 人卵巢腺癌细胞；OVCAR-3 epithelial-like 上皮细胞样 adherent growth 贴壁生长 OVCAR-3 cells were derived from ovarian tumor tissue of a 60-year-old white female and were established by T. C. Hamiltonyu in 1982. This cell carries the estrogen and androgen receptor. It is resistant to adriamycin, cisplatin and melphalan, and suitable for the study of drug resistance of ovarian cancer. The number of OVCAR-3 chromosomes is within the range of subtriploid. OVCAR-3细胞源自一名60岁白人女性卵巢肿瘤组织，由T.C.Hamiltonyu建系于1982年。该细胞带有雌、雄激素受体。对阿霉素，顺氯氨铂，(左旋)苯丙氨酸氮芥有一定抗药性，适用于卵巢癌的抗药性研究。OVCAR-3染色体数量在亚三倍体范围内。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C13 C13 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR890 OVCAR-3 OVCAR-3 cell OVCAR-3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR890 cell NICR890 细胞 http://www.cellresource.cn/fdetail.aspx?id=890 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：11，12；D13S317：12；D16S539：12；D18S51：13；D19S433：16.2；D21S11：29，31.2；D2S1338：17，21；D3S1358：17，18；D5S818：11，12；D7S820：10；D8S1179：10，15；FGA：21；TH01：9，9.3；TPOX：8；vWA：17； Amelogenin：X；CSF1PO：11，12；D13S317：12；D16S539：12；D18S51：13；D19S433：16.2；D21S11：29，31.2；D2S1338：17，21；D3S1358：17，18；D5S818：11，12；D7S820：10；D8S1179：10，15；FGA：21；TH01：9，9.3；TPOX：8；vWA：17； 3111C0001CCC000362 Human Ovarian Adenocarcinoma Cells; OVCAR-3 人卵巢腺癌细胞；OVCAR-3 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were established in 1982 by T.C. Hamilton et al., derived from an ascites of a 60-year-old patient with ovarian adenocarcinoma, and is a model for the study of drug resistance in ovarian cancer. 该细胞1982年由T.C. Hamilton等建系，源自一位60卵巢腺癌的腹水，是卵巢癌抗药性研究的模型。 RPMI 1640 (w/o Hepes) 20% FBS RPMI 1640 (w/o Hepes) 20% FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P30 P30 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） Neartriploid 近三倍体 A类 Class A OVCAR-3-Cell Morphology-PUMC OVCAR-3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:4传代，每周换液2～3次 Passage at 1: 2 ~ 1: 4, and change fluid for 2 to 3 times a week NICR1551 OVCAR-3 OVCAR-3 cell OVCAR-3 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1551 cell NICR1551 细胞 http://www.cellresource.cn/fdetail.aspx?id=1551 RPMI 1640 (w/o Hepes) 20% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 20% FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf OVCAR-3-Cell Morphology-PUMC OVCAR-3-细胞形态-PUMC Amelogenin：X，Y；CSF1PO：11；D13S317：8，11；D16S539：12；D18S51：16，17，18；D19S433：14，14.2；D21S11：30，31.2；D2S1338：18，23；D3S1358：14；D5S818：11；D7S820：13，14；D8S1179：14，15；FGA：24，25；TH01：9，9.3；TPOX：8，11；vWA：17，18； Amelogenin：X，Y；CSF1PO：11；D13S317：8，11；D16S539：12；D18S51：16，17，18；D19S433：14，14.2；D21S11：30，31.2；D2S1338：18，23；D3S1358：14；D5S818：11；D7S820：13，14；D8S1179：14，15；FGA：24，25；TH01：9，9.3；TPOX：8，11；vWA：17，18； 3111C0001CCC000027 Human Ovarian Adenocarcinoma Cells; SK-OV-3 人卵巢腺癌细胞；SK-OV-3 epithelioid 上皮样 adherent growth 贴壁生长 SK-OV-3 was isolated from the peritoneal effusion of a 64-year-old white female patient with ovarian adenocarcinoma by Trempe G and Old LJ in 1973. The cells are resistant to tumor necrosis factor and several cytotoxic drugs, including diphtheria toxin, cisplatin and adriamycin. The cells form tumors in nude mice and form moderately differentiated adenocarcinoma consistent with ovarian carcinoma in situ. SK-OV-3由Trempe G和Old LJ在1973年从一位64岁白人女性卵巢腺癌患者的腹腔积液分离得到。该细胞对肿瘤坏死因子和几种细胞毒性药物（包括白喉毒素、顺铂和阿霉素）耐受。在裸鼠中成瘤，且形成与卵巢原位癌一致的中度分化的腺癌。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A SK-OV-3-Cell Morphology-PUMC SK-OV-3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:6传代；每周2~3次。 Passage at 1: 2 ~ 1: 6; and passage for 2 to 3 times per week. NICR27 SK-OV-3 SK-OV-3 cell SK-OV-3 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR27 cell NICR27 细胞 http://www.cellresource.cn/fdetail.aspx?id=27 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf SK-OV-3-Cell Morphology-PUMC SK-OV-3-细胞形态-PUMC Amelogenin:X；CSF1PO:11；D13S317:8,11；D16S539:12；D18S51:16,17,18；D19S433:14,14.2；D21S11:30,31.2；D2S1338:18,23；D3S1358:14；D5S818:11；D7S820:13,14；D8S1179:14,15；FGA:24,25；TH01:9,9.3；TPOX:8,11；vWA:17,18 Amelogenin:X；CSF1PO:11；D13S317:8,11；D16S539:12；D18S51:16,17,18；D19S433:14,14.2；D21S11:30,31.2；D2S1338:18,23；D3S1358:14；D5S818:11；D7S820:13,14；D8S1179:14,15；FGA:24,25；TH01:9,9.3；TPOX:8,11；vWA:17,18 3131C0001000700107 Human Ovarian Adenocarcinoma Cells; SK-OV-3 人卵巢腺癌细胞；SK-OV-3 epithelioid 上皮样 adherent growth 贴壁生长 SK-OV-3 was isolated from the peritoneal effusion of a 64-year-old white female patient with ovarian adenocarcinoma by Trempe G and Old LJ in 1973. The cells are resistant to tumor necrosis factor and several cytotoxic drugs, including diphtheria toxin, cisplatin and adriamycin. The cells form tumors in nude mice and form moderately differentiated adenocarcinoma consistent with ovarian carcinoma in situ. SK-OV-3由Trempe G和Old LJ在1973年从一位64岁白人女性卵巢腺癌患者的腹腔积液分离得到。该细胞对肿瘤坏死因子和几种细胞毒性药物（包括白喉毒素、顺铂和阿霉素）耐受。在裸鼠中成瘤，且形成与卵巢原位癌一致的中度分化的腺癌。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P24 P24 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B SK-OV-3-DNA-Fingerprinting-SHCRC SK-OV-3-DNA-指纹-SHCRC SK-OV-3-cell morphology-SHCRC SK-OV-3-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR675 SK-OV-3 SK-OV-3 cell SK-OV-3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR675 cell NICR675 细胞 http://www.cellresource.cn/fdetail.aspx?id=675 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf SK-OV-3-DNA-Fingerprinting-SHCRC SK-OV-3-DNA-指纹-SHCRC SK-OV-3-cell morphology-SHCRC SK-OV-3-细胞形态-SHCRC 3142C0001000000090 Human Ovarian Adenocarcinoma Cells; SK-OV-3 人卵巢腺癌细胞；SK-OV-3 epithelial-like 上皮细胞样 adherent growth 贴壁生长 SK-OV-3 was isolated from the peritoneal effusion of a 64-year-old white female patient with ovarian adenocarcinoma by Trempe G and Old LJ in 1973. The cells are resistant to tumor necrosis factor and several cytotoxic drugs, including diphtheria toxin, cisplatin and adriamycin. The cells form tumors in nude mice and form moderately differentiated adenocarcinoma consistent with ovarian carcinoma in situ. SK-OV-3由Trempe G和Old LJ在1973年从一位64岁白人女性卵巢腺癌患者的腹腔积液分离得到。该细胞对肿瘤坏死因子和几种细胞毒性药物（包括白喉毒素、顺铂和阿霉素）耐受。在裸鼠中成瘤，且形成与卵巢原位癌一致的中度分化的腺癌。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts)  10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts)  10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C26 C26 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR858 SK-OV-3 SK-OV-3 cell SK-OV-3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR858 cell NICR858 细胞 http://www.cellresource.cn/fdetail.aspx?id=858 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts)  10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts)  10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin:X；CSF1PO:11；D13S317:8,11；D16S539:12；D18S51:16,17,18；D19S433:14,14.2；D21S11:30,31.2；D2S1338:18,23；D3S1358:14；D5S818:11；D7S820:13,14；D8S1179:14,15；FGA:24,25；TH01:9,9.3；TPOX:8,11；vWA:17,18 Amelogenin:X；CSF1PO:11；D13S317:8,11；D16S539:12；D18S51:16,17,18；D19S433:14,14.2；D21S11:30,31.2；D2S1338:18,23；D3S1358:14；D5S818:11；D7S820:13,14；D8S1179:14,15；FGA:24,25；TH01:9,9.3；TPOX:8,11；vWA:17,18 3131C0001000700185 Human Ovarian Adenocarcinoma Cells; SK-OV-3 [SKOV3] 人卵巢腺癌细胞；SK-OV-3 [SKOV3] epithelial cell 上皮细胞 adherent growth 贴壁生长 SK-OV-3 was isolated from the ascites of ovarian cancer patients by G.Trempe and L.J. Old in 1973. The cells were resistant to tumor necrosis factor and several cytotoxic drugs including diphtheria toxin, cisplatin and adriamycin. The cells are tumorigenic in nude mice and form moderately differentiated adenocarcinoma consistent with ovarian carcinoma in situ. SK-OV-3由G.Trempe和L.J.Old在1973年从卵巢肿瘤病人的腹水分离得到。 此细胞对肿瘤坏死因子和几种细胞毒性药物包括白喉毒素、顺铂和阿霉素均耐受。 在裸鼠中致瘤，且形成与卵巢原位癌一致的中度分化的腺癌。 McCoy＇s 5A Media (Modified with Tricine) qualified fetal bovine serum，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P(25+5) P(25+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A SK-OV-3-DNA-Fingerprinting-SHCRC SK-OV-3-DNA-指纹-SHCRC SK-OV-3-cell morphology-SHCRC SK-OV-3-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1695 SK-OV-3 SK-OV-3 cell SK-OV-3 细胞 SKOV3 SKOV3 cell SKOV3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1695 cell NICR1695 细胞 http://www.cellresource.cn/fdetail.aspx?id=1695 McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100925.pdf SK-OV-3-DNA-Fingerprinting-SHCRC SK-OV-3-DNA-指纹-SHCRC SK-OV-3-cell morphology-SHCRC SK-OV-3-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000118 Human Ovarian Adenocarcinoma Cells; SW626 人卵巢腺癌细胞；SW626 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line was derived from a 65-year-old white female endometrial adenocarcinoma tissue and was established by DL Way in 1983. The cells express α-keratin and have microvilli on the cell surface. 该细胞系源自一名65岁白人女性子宫内膜腺癌组织，1983年由DL Way建系。该细胞表达α-角蛋白，细胞表面具有微绒毛。 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS http://www.cellresource.cn/pdf/20080108100542.pdf http://www.cellresource.cn/pdf/20080108100542.pdf C17 C17 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR886 SW626 SW626 cell SW626 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR886 cell NICR886 细胞 http://www.cellresource.cn/fdetail.aspx?id=886 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100542.pdf Amelogenin：X；CSF1PO：9，10；D13S317：8；D16S539：11，12；D18S51：15，16；D19S433：14，14.2；D21S11：29，30，31；D2S1338：17；D3S1358：16；D5S818：11，12；D7S820：9，11；D8S1179：12；FGA：21，24；TH01：9.3；TPOX：8，9；vWA：16； Amelogenin：X；CSF1PO：9，10；D13S317：8；D16S539：11，12；D18S51：15，16；D19S433：14，14.2；D21S11：29，30，31；D2S1338：17；D3S1358：16；D5S818：11，12；D7S820：9，11；D8S1179：12；FGA：21，24；TH01：9.3；TPOX：8，9；vWA：16； 3111C0001CCC000039 Human Chronic Myeloid Leukemia Cells; K562 人慢性髓系白血病细胞；K562 lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 The cells were isolated by Lozzio from the hydrothorax of a 53-year-old woman with chronic myeloid leukemia in acute phase. The cell was thought to have originated from the granular system and was in a highly undifferentiated stage. Anderson et al considered that it was a erythroleukemia cell line after the study of the cell membrane characteristics. This cell is a highly sensitive in vitro target to natural killer cells and is therefore widely used in this field. The primitive cell of K562 is a malignant tumor cell of hemopoietic system with multidirectional differentiation potential, which can spontaneously differentiate into the identifiable progenitor cells of erythroid, granulocyte and monocyte. The cells express CD7 (25%). 该细胞是由Lozzio从一名53岁的慢性髓细胞性白血病急变期的女性患者的胸水中分离建立的。该细胞曾被认为来源于粒系，处于高度未分化阶段；Anderson等人作了细胞膜特性的研究后，认为该细胞是红白血病细胞系。该细胞是对自然杀伤细胞高度敏感的体外靶标，故而被广泛应用于这方面的研究。K562的原始细胞是一种具有多向分化潜能的造血系统的恶性肿瘤细胞，能自发分化为红系、粒系和单核系的可辨识的祖细胞。该细胞表达CD7（25％）。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 67~68 67~68 A类 Class A K562-Species Identification-PUMC K562-cell morphology-PUMC K562-物种鉴定-PUMC K562-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 1 � 105 ~ 1 � 106/ml, and change fluid once every 2~3 day. 维持细胞浓度在1×105~1×106/ml,每2~3天换液1次。 K562 K562 cell K562 细胞 NICR39 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR39 cell NICR39 细胞 http://www.cellresource.cn/fdetail.aspx?id=39 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf K562-Species Identification-PUMC K562-cell morphology-PUMC K562-物种鉴定-PUMC K562-细胞形态-PUMC Amelogenin:X；CSF1PO:9,10；D13S317:8；D16S539:11,12；D18S51:15,16；D21S11:29,31；D3S1358:16；D5S818:11,12；D7S820:9,11；D8S1179:12；FGA:21,24；PentaD:9,13；PentaE:5,14；TH01:9.3；TPOX:8,9；vWA:16 Amelogenin:X；CSF1PO:9,10；D13S317:8；D16S539:11,12；D18S51:15,16；D21S11:29,31；D3S1358:16；D5S818:11,12；D7S820:9,11；D8S1179:12；FGA:21,24；PentaD:9,13；PentaE:5,14；TH01:9.3；TPOX:8,9；vWA:16 3111C0002000000026 Human Chronic Myeloid Leukemia Cells; K562 人慢性髓系白血病细胞；K562 lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 The cells were isolated by Lozzio from the hydrothorax of a 53-year-old woman with chronic myeloid leukemia in acute phase. The cell was thought to have originated from the granular system and was in a highly undifferentiated stage. Anderson et al considered that it was a erythroleukemia cell line after the study of the cell membrane characteristics. This cell is a highly sensitive in vitro target to natural killer cells and is therefore widely used in this field. The primitive cell of K562 is a malignant tumor cell of hemopoietic system with multidirectional differentiation potential, which can spontaneously differentiate into the identifiable progenitor cells of erythroid, granulocyte and monocyte. The cells express CD7 (25%). 该细胞是由Lozzio从一名53岁的慢性髓细胞性白血病急变期的女性患者的胸水中分离建立的。该细胞曾被认为来源于粒系，处于高度未分化阶段；Anderson等人作了细胞膜特性的研究后，认为该细胞是红白血病细胞系。该细胞是对自然杀伤细胞高度敏感的体外靶标，故而被广泛应用于这方面的研究。K562的原始细胞是一种具有多向分化潜能的造血系统的恶性肿瘤细胞，能自发分化为红系、粒系和单核系的可辨识的祖细胞。该细胞表达CD7（25％）。 RPMI 1640 (w/o Hepes) new-born calf serum，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A K 562-cell morphology-NIFDC K 562-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net Maintain the cell concentration between 1 � 105 ~ 1 � 106/ml, and change fluid once every 2~3 day. 维持细胞浓度在1×105~1×106/ml,每2~3天换液1次。 K562 K562 cell K562 细胞 NICR398 NICR398 cell NICR398 细胞 http://www.cellresource.cn/fdetail.aspx?id=398 RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 新生牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf K 562-cell morphology-NIFDC K 562-细胞形态-NIFDC Amelogenin:X；CSF1PO:9,10；D13S317:8；D16S539:11,12；D18S51:15,16；D19S433:14,14.2；D21S11:29,30,31；D2S1338:17；D3S1358:16；D5S818:11,12；D7S820:9,11；D8S1179:12；FGA:21,24；TH01:9.3；TPOX:8,9；vWA:16 Amelogenin:X；CSF1PO:9,10；D13S317:8；D16S539:11,12；D18S51:15,16；D19S433:14,14.2；D21S11:29,30,31；D2S1338:17；D3S1358:16；D5S818:11,12；D7S820:9,11；D8S1179:12；FGA:21,24；TH01:9.3；TPOX:8,9；vWA:16 3131C0001000700029 Human Chronic Myeloid Leukemia Cells; K562 人慢性髓系白血病细胞；K562 lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 Continuous cell line K-562 was established by Lozzio from the hydrothorax of a dying 53-year-old female patient with chronic myeloid leukemia. The cells were classified as highly undifferentiated granular white blood cells. Anderson et al showed that K-562 is a human erythroleukemia cell by studying its surface membrane properties. K-562 cell lines are widely used as highly sensitive receptors in vitro in natural killer analysis. Se Cross et al. used it for detailed detection of NK cells in vitro, including mathematical quantification of NK cell activity. K-562 mother cells are pluripotent, hematopoietic malignant cells that spontaneously differentiate into identifiable red blood cell progenitor cells, granular white blood cells, monocytes, and so on. It is EBNA-negative. 连续细胞株K-562由Lozzio从一位临终的53岁女性慢性骨髓性白血病患者的胸水中建立。 细胞被归入高度未分化的粒性白细胞类。 Anderson等对其表面膜性质的研究表明，K-562是一株人类红白血病细胞。 K-562细胞株在自然杀伤分析中广泛作为高敏感的体外受体。 See Pross等将它用于NK细胞的体外详细检测，包括NK细胞活性的数学量化。 K-562母细胞是多能性，造血恶性细胞，它能自发分化成可识别的红血球祖细胞、粒性白细胞、单核细胞等。它是EBNA阴性的。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，10% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B K562-cell morphology-SHCRC K562-细胞形态-SHCRC LDH检测报告K562-Isoenzyme-SHCRC LDH检测报告K562-同工酶-SHCRC MDH检测报告K562-Isoenzyme-SHCRC MDH检测报告K562-同工酶-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. K562 K562 cell K562 细胞 NICR599 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR599 cell NICR599 细胞 http://www.cellresource.cn/fdetail.aspx?id=599 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100822.pdf K562-cell morphology-SHCRC K562-细胞形态-SHCRC LDH检测报告K562-Isoenzyme-SHCRC LDH检测报告K562-同工酶-SHCRC MDH检测报告K562-Isoenzyme-SHCRC MDH检测报告K562-同工酶-SHCRC STR Identification STR鉴定 3142C0001000000037 Human Chronic Myeloid Leukemia Cells; K562 人慢性髓系白血病细胞；K562 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 The cells were isolated by Lozzio from the hydrothorax of a 53-year-old woman with chronic myeloid leukemia in acute phase. The cell was thought to have originated from the granular system and was in a highly undifferentiated stage. Anderson et al considered that it was a erythroleukemia cell line after the study of the cell membrane characteristics. This cell is a highly sensitive in vitro target to natural killer cells and is therefore widely used in this field. The primitive cell of K562 is a malignant tumor cell of hemopoietic system with multidirectional differentiation potential, which can spontaneously differentiate into the identifiable progenitor cells of erythroid, granulocyte and monocyte. The cells express CD7 (25%). 该细胞是由Lozzio从一名53岁的慢性髓细胞性白血病急变期的女性患者的胸水中分离建立的。该细胞曾被认为来源于粒系，处于高度未分化阶段；Anderson等人作了细胞膜特性的研究后，认为该细胞是红白血病细胞系。该细胞是对自然杀伤细胞高度敏感的体外靶标，故而被广泛应用于这方面的研究。K562的原始细胞是一种具有多向分化潜能的造血系统的恶性肿瘤细胞，能自发分化为红系、粒系和单核系的可辨识的祖细胞。该细胞表达CD7（25％）。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C104 C104 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. K562 K562 cell K562 细胞 NICR805 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR805 cell NICR805 细胞 http://www.cellresource.cn/fdetail.aspx?id=805 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X；D8S1179：12；D21S11：29， 31；D7S820：9，11；CSF1PO：9，10；D3S1358：16；TH01：9.3；D13S317：8；D16S539：11，12；D2S1338：17；D19S433：14,14.2；vWA：16；THOX：8, 9；D18S51：15,16；D5S818：11,12；FGA：21, 24。 Amelogenin：X；D8S1179：12；D21S11：29， 31；D7S820：9，11；CSF1PO：9，10；D3S1358：16；TH01：9.3；D13S317：8；D16S539：11，12；D2S1338：17；D19S433：14,14.2；vWA：16；THOX：8, 9；D18S51：15,16；D5S818：11,12；FGA：21, 24。 3153C0001000000012 Human Chronic Myeloid Leukemia Cells; K562 人慢性髓系白血病细胞；K562 lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 This cell line was isolated and established by Lozzio and Lozzio in 1975 from the hydrothorax of a 53-year-old chronic myeloid leukemia female patient. This cell line has been widely used in the study of natural killing. K-562 mother cell is a malignant tumor cell with multipotent hematopoietic system, which can spontaneously differentiate into identifiable progenitor cells of erythroid, granulocyte and monocyte; CD7 is expressed. 该细胞系由Lozzio and Lozzio 于1975年从一名53岁慢性髓系白血病女性患者的胸水中分离建立。此细胞株被广泛用于自然杀伤方面的研究。K-562母细胞是一种具多能性的造血系统的恶性肿瘤细胞，能自发分化成可识别的红系、粒系和单核系的祖细胞；表达CD7。 RPMI 1640 (w/o Hepes) 15%NBS RPMI 1640 (w/o Hepes) 15%NBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN12 PN12 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） 59-68 59-68 A类 Class A K562-cell morphology-KCB K562-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1：3传代；3-5天一次 Passage at 1: 3; and passage once in 3-5 days K562 K562 cell K562 细胞 NICR1286 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1286 cell NICR1286 细胞 http://www.cellresource.cn/fdetail.aspx?id=1286 RPMI 1640 (w/o Hepes) 15%NBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 15%NBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf K562-cell morphology-KCB K562-细胞形态-KCB Amelogenin:X；CSF1PO:9,10；D13S317:8；D16S539:11,12；D18S51:15,16；D19S433:14,14.2；D21S11:29,30,31；D2S1338:17；D3S1358:16；D5S818:11,12；D7S820:9,11；D8S1179:12；FGA:21,24；TH01:9.3；TPOX:8,9；vWA:16 Amelogenin:X；CSF1PO:9,10；D13S317:8；D16S539:11,12；D18S51:15,16；D19S433:14,14.2；D21S11:29,30,31；D2S1338:17；D3S1358:16；D5S818:11,12；D7S820:9,11；D8S1179:12；FGA:21,24；TH01:9.3；TPOX:8,9；vWA:16 3131C0001000700191 Human Chronic Myeloid Leukemia Cells; K562 人慢性髓系白血病细胞；K562 lymphoblast-like; round 淋巴母细胞样；圆形 suspension growth 悬浮生长 Continuous cell line K-562 was established by Lozzio from the hydrothorax of a dying 53-year-old female patient with chronic myeloid leukemia. The cells were classified as highly undifferentiated granular white blood cells. Anderson et al showed that K-562 is a human erythroleukemia cell by studying its surface membrane properties. K-562 cell lines are widely used as highly sensitive receptors in vitro in natural killer analysis. Se Cross et al. used it for detailed detection of NK cells in vitro, including mathematical quantification of NK cell activity. K-562 mother cells are pluripotent, hematopoietic malignant cells that spontaneously differentiate into identifiable red blood cell progenitor cells, granular white blood cells, monocytes, and so on. It is EBNA-negative. 连续细胞株K-562由Lozzio从一位临终的53岁女性慢性骨髓性白血病患者的胸水中建立。 细胞被归入高度未分化的粒性白细胞类。 Anderson等对其表面膜性质的研究表明，K-562是一株人类红白血病细胞。 K-562细胞株在自然杀伤分析中广泛作为高敏感的体外受体。 See Pross等将它用于NK细胞的体外详细检测，包括NK细胞活性的数学量化。 K-562母细胞是多能性，造血恶性细胞，它能自发分化成可识别的红血球祖细胞、粒性白细胞、单核细胞等。它是EBNA阴性的。 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，10% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100822.pdf http://www.cellresource.cn/pdf/20080108100822.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B K562-cell morphology-SHCRC K562-细胞形态-SHCRC LDH检测报告K562-Isoenzyme-SHCRC LDH检测报告K562-同工酶-SHCRC MDH检测报告K562-Isoenzyme-SHCRC MDH检测报告K562-同工酶-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. K562 K562 cell K562 细胞 NICR2094 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2094 cell NICR2094 细胞 http://www.cellresource.cn/fdetail.aspx?id=2094 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100822.pdf IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100822.pdf K562-cell morphology-SHCRC K562-细胞形态-SHCRC LDH检测报告K562-Isoenzyme-SHCRC LDH检测报告K562-同工酶-SHCRC MDH检测报告K562-Isoenzyme-SHCRC MDH检测报告K562-同工酶-SHCRC 3142C0001000000149 Normal Human Foreskin Fibroblasts; Hs68 人男性正常龟头细胞；Hs68 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 The cells were established by Owens RB in 1969. 该细胞1969年由Owens RB建立。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C6 C6 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Hs68 Hs68 cell Hs68 细胞 NICR917 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR917 cell NICR917 细胞 http://www.cellresource.cn/fdetail.aspx?id=917 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin：X，Y；CSF1PO：9，13；D13S317：8，12；D16S539：9，10；D18S51：12，14；D19S433：14；D21S11：27，33.2；D2S1338：18，20；D3S1358：14，16；D5S818：11，12；D7S820：11；D8S1179：12，13；FGA：21.2，22；TH01：6，8；TPOX：8，11；vWA：18，20； Amelogenin：X，Y；CSF1PO：9，13；D13S317：8，12；D16S539：9，10；D18S51：12，14；D19S433：14；D21S11：27，33.2；D2S1338：18，20；D3S1358：14，16；D5S818：11，12；D7S820：11；D8S1179：12，13；FGA：21.2，22；TH01：6，8；TPOX：8，11；vWA：18，20； 3111C0001CCC000345 Human Glioma Cells; HS 683 人脑胶质瘤细胞；HS 683 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 The cells are derived from left temporal lobe gliomas in 76-year-old white men, with microvilli and no desmosomes. 该细胞源自76岁白人男性的左颞叶侧胶质瘤组织，有微绒毛，无桥粒。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P30 P30 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 74～87 74～87 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4传代，每周换液2次 Passage at 1: 4, and change fluid for 2 times a week HS 683 HS 683 cell HS 683 细胞 NICR1534 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1534 cell NICR1534 细胞 http://www.cellresource.cn/fdetail.aspx?id=1534 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：12；D13S317：11；D16S539：9，12；D18S51：13，19；D19S433：13，14；D21S11：27，32.2；D2S1338：16，17；D3S1358：17，18；D5S818：11；D7S820：8，10；D8S1179：12，13；FGA：21，24；TH01：6，7；TPOX：8，11；vWA：14，17； Amelogenin：X；CSF1PO：12；D13S317：11；D16S539：9，12；D18S51：13，19；D19S433：13，14；D21S11：27，32.2；D2S1338：16，17；D3S1358：17，18；D5S818：11；D7S820：8，10；D8S1179：12，13；FGA：21，24；TH01：6，7；TPOX：8，11；vWA：14，17； 3111C0001CCC000049 Human Brain Tumor Cells; SF126 人脑瘤细胞；SF126 fibroblast-like 成纤维样 adherent growth 贴壁生长 This cell is derived from astrocytoma; test for glial fibrillary acidic protein (GFAP) is negative; the cells can specifically bind β-endorphin. 该细胞来源于星形胶质细胞瘤；胶质纤维酸性蛋白（GFAP）阴性；可以特异地结合β-内啡肽。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 43~47 43~47 A类 Class A SF126-Cell Morphology-PUMC SF126-Species Identification-PUMC SF126-cell morphology-PUMC SF126-物种鉴定-PUMC SF126-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3传代；3~4天1次。　 Passage at 1: 3; and passage once in 3 to 4 days NICR49 SF126 SF126 cell SF126 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR49 cell NICR49 细胞 http://www.cellresource.cn/fdetail.aspx?id=49 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf SF126-Cell Morphology-PUMC SF126-Species Identification-PUMC SF126-cell morphology-PUMC SF126-物种鉴定-PUMC SF126-细胞形态-PUMC SF126-细胞形态-PUMC Amelogenin：X，Y；CSF1PO：9，12；D13S317：8，10；D16S539：11；D18S51：16，18；D19S433：13，14；D21S11：28，30.2；D2S1338：17，23；D3S1358：15；D5S818：11，OL；D7S820：10；D8S1179：12，13；FGA：20，23；TH01：7；TPOX：8，11；vWA：16，18； Amelogenin：X，Y；CSF1PO：9，12；D13S317：8，10；D16S539：11；D18S51：16，18；D19S433：13，14；D21S11：28，30.2；D2S1338：17，23；D3S1358：15；D5S818：11，OL；D7S820：10；D8S1179：12，13；FGA：20，23；TH01：7；TPOX：8，11；vWA：16，18； 3111C0001CCC000341 Human Medulloblastoma Cells; D283 人脑髓母细胞瘤细胞；D283 epithelial-like 上皮细胞样 suspension growth, in clusters, A few adherent 悬浮生长，聚团，少数贴壁 The cells were established in 1985 by Friedman et al., and were derived from ascites cells from a 6-year-old white male child with neurocytoma and abdominal metastasis. 该细胞1985年由Friedman等建系，源自一名6岁患有神经管细胞瘤有腹腔转移的白人男性小孩的腹水细胞。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C2 C2 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 45，XY 45，XY A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell density in the 4 � 105 ~ 1 � 106cells/ml, changing fluid for 2 to 3 times a week 保持细胞密度在4×105～1×106cells/ml之间，每周换液2～3次 D283 D283 cell D283 细胞 NICR1530 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1530 cell NICR1530 细胞 http://www.cellresource.cn/fdetail.aspx?id=1530 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001001200022 Human Brain Astrocytoma; U-118 MG 人脑星形胶质母细胞瘤；U-118 MG mixed 混合形 adherent growth 贴壁生长 Note: It is reported that glioblastoma cell lines U-118 MG (HTB-15) and U-138 MG (HTB-16) from different individuals have consistent VNTR and similar STR patterns. U-118 MG and U-138 MG are cytogenetically similar and have at least six derived marker chromosomes. This was one of the cell lines constructed by J. Ponten and colleagues from malignant gliomas (others including ATCC HTB-14, ATCC HTB-16 and ATCC HTB-17) during 1966 ~ 1969. Mycoplasma contamination was removed by BM-Cyline culture for 6 weeks in 1987. 注意： 据报道来自不同个体的胶质母细胞瘤细胞株U-118 MG (HTB-15) 和 U-138 MG (HTB-16)有着一致的VNTR和相近的STR模式。 U-118 MG 和 U-138 MG细胞遗传学上很相似并有至少六个衍生标记染色体。 这是1966年至1969年间J. Ponten和同事从恶性神经胶质瘤中构建的细胞株中的一株(其它包括ATCC HTB-14和ATCC HTB-16 and ATCC HTB-17)。 1987年用BM-Cycline培养6周去除了支原体污染。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B U-118MG-cell morphology-SHCRC U-118MG-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen Unit: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences Zip code: 200032 Address: No. 320, Yueyang Road, Shanghai Tel: 021-54920404，021-54920405 Fax: 021-54920406 Email: shchen@sibs.ac.cn 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920406 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1603 U-118 MG U-118 MG cell U-118 MG 细胞 Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744 Fogh J, et al. Absence of HeLa cell contamination in Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744 Fogh J, et al. Absence of HeLa cell contamination in The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1603 cell NICR1603 细胞 http://www.cellresource.cn/fdetail.aspx?id=1603 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf U-118MG-cell morphology-SHCRC U-118MG-细胞形态-SHCRC 3131C0001000700216 Human Brain Astrocytoma; U-118 MG 人脑星形胶质母细胞瘤；U-118 MG mixed 混合形 adherent growth 贴壁生长 Note: It is reported that glioblastoma cell lines U-118 MG (HTB-15) and U-138 MG (HTB-16) from different individuals have consistent VNTR and similar STR patterns. U-118 MG and U-138 MG are cytogenetically similar and have at least six derived marker chromosomes. This was one of the cell lines constructed by J. Ponten and colleagues from malignant gliomas (others including ATCC HTB-14, ATCC HTB-16 and ATCC HTB-17) during 1966 ~ 1969. Mycoplasma contamination was removed by BM-Cyline culture for 6 weeks in 1987. 注意： 据报道来自不同个体的胶质母细胞瘤细胞株U-118 MG (HTB-15) 和 U-138 MG (HTB-16)有着一致的VNTR和相近的STR模式。 U-118 MG 和 U-138 MG细胞遗传学上很相似并有至少六个衍生标记染色体。 这是1966年至1969年间J. Ponten和同事从恶性神经胶质瘤中构建的细胞株中的一株(其它包括ATCC HTB-14和ATCC HTB-16 and ATCC HTB-17)。 1987年用BM-Cycline培养6周去除了支原体污染。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P(446+5) P(446+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B U-118MG-cell morphology-SHCRC U-118MG-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen Unit: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences Zip code: 200032 Address: No. 320, Yueyang Road, Shanghai Tel: 021-54920404，021-54920405 Fax: 021-54920406 Email: shchen@sibs.ac.cn 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920406 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR2099 U-118 MG U-118 MG cell U-118 MG 细胞 Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744 Fogh J, et al. Absence of HeLa cell contamination in Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744 Fogh J, et al. Absence of HeLa cell contamination in The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2099 cell NICR2099 细胞 http://www.cellresource.cn/fdetail.aspx?id=2099 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf U-118MG-cell morphology-SHCRC U-118MG-细胞形态-SHCRC Amelogenin：X；CSF1PO：10，11；D13S317：8，11；D16S539：12；D18S51：13，14；D19S433：15，15.2；D21S11：28，32.2；D2S1338：20，23；D3S1358：16，17；D5S818：11，12；D7S820：8，9；D8S1179：10，11；FGA：18，24；TH01：9.3；TPOX：8；vWA：15，17； Amelogenin：X；CSF1PO：10，11；D13S317：8，11；D16S539：12；D18S51：13，14；D19S433：15，15.2；D21S11：28，32.2；D2S1338：20，23；D3S1358：16，17；D5S818：11，12；D7S820：8，9；D8S1179：10，11；FGA：18，24；TH01：9.3；TPOX：8；vWA：15，17； 3111C0001CCC000208 Human Brain Astrocytoma; U-87 MG [U87MG; U87 MG] 人脑星形胶质母细胞瘤；U-87 MG [U87MG；U87 MG] epithelioid 上皮样 adherent growth 贴壁生长 This cell line was established by Ponten J et al and originated from malignant glioma. Tumor can be formed by subcutaneous inoculation in nude mice. 该细胞系由Ponten J等建立，源于恶性神经胶质瘤。裸鼠皮下接种可成瘤。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1% NEAA MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1% NEAA http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P135 P135 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A U87MG-Cell Morphology-PUMC U87MG-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:5传代；2~3天换液1次。 Passage at 1: 2 ~ 1: 5; and change fluid once in 2~3 days. NICR208 U-87 MG U-87 MG cell U-87 MG 细胞 U87 MG U87 MG cell U87 MG 细胞 U87MG U87MG cell U87MG 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR208 cell NICR208 细胞 http://www.cellresource.cn/fdetail.aspx?id=208 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1% NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1% NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf U87MG-Cell Morphology-PUMC U87MG-细胞形态-PUMC Amelogenin:X；CSF1PO:10,11；D13S317:8,11；D16S539:12；D18S51:13,14；D19S433:15,15.2；D21S11:28,32.2；D2S1338:20,23；D3S1358:16,17；D5S818:11,12；D7S820:8,9；D8S1179:10,11；FGA:18,24；TH01:9.3；TPOX:8；vWA:15,17 Amelogenin:X；CSF1PO:10,11；D13S317:8,11；D16S539:12；D18S51:13,14；D19S433:15,15.2；D21S11:28,32.2；D2S1338:20,23；D3S1358:16,17；D5S818:11,12；D7S820:8,9；D8S1179:10,11；FGA:18,24；TH01:9.3；TPOX:8；vWA:15,17 3131C0001000700138 Human Brain Astrocytoma; U-87 MG [U87MG; U87 MG] 人脑星形胶质母细胞瘤；U-87 MG [U87MG；U87 MG] epithelial cell 上皮细胞 adherent growth 贴壁生长 This was one of the cell lines constructed by J. Ponten and colleagues from malignant gliomas (others including ATCC HTB-15, ATCC HTB-16 and ATCC HTB-17) during 1966 ~ 1969. Mycoplasma contamination was eliminated in September 1975. 这是1966年至1969年间J. Ponten和同事从恶性神经胶质瘤中构建的细胞株中的一株(其它包括ATCC HTB-15, ATCC HTB-16 and ATCC HTB-17)。 1975九月消除了支原体污染。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A U87MG-cell morphology-SHCRC U87MG-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR706 U-87 MG U-87 MG cell U-87 MG 细胞 U87 MG U87 MG cell U87 MG 细胞 U87MG U87MG cell U87MG 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR706 cell NICR706 细胞 http://www.cellresource.cn/fdetail.aspx?id=706 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf U87MG-cell morphology-SHCRC U87MG-细胞形态-SHCRC Amelogenin：X，Y；CSF1PO：11；D13S317：11；D16S539：9；D18S51：16，18；D19S433：13，15；D21S11：36；D2S1338：25；D3S1358：15，17；D5S818：11，12；D7S820：10，11；D8S1179：10，16；FGA：22；TH01：7，9；TPOX：8；vWA：18； Amelogenin：X，Y；CSF1PO：11；D13S317：11；D16S539：9；D18S51：16，18；D19S433：13，15；D21S11：36；D2S1338：25；D3S1358：15，17；D5S818：11，12；D7S820：10，11；D8S1179：10，16；FGA：22；TH01：7，9；TPOX：8；vWA：18； 3111C0001CCC000242 Human Bladder Cancer Cells; 5637（HTB-9） 人膀胱癌细胞；5637（HTB-9） epithelioid 上皮样 adherent growth 贴壁生长 The cells are derived from a 68-year-old white male with bladder cancer. It is reported that the cells can produce SCF, IL-1, IL-3, IL-6, G-CSF, GM-CSF and so on. 该细胞源于一位68岁的患有膀胱癌的白人男性患者。据报道，该细胞能产生 SCF、IL-1、IL-3、IL-6、G-CSF、GM-CSF等。 RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mM Hepes＋1mM sodium pyruvate RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mM Hepes＋1mM sodium pyruvate http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 49~64 49~64 A类 Class A 5637-cell morphology-PUMC 5637-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:8传代；每周换液2~3次。 Passage at 1: 4 ~ 1: 8; and change fluid for 2~3 times a week. 5637（HTB-9） 5637（HTB-9） cell 5637（HTB-9） 细胞 NICR242 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR242 cell NICR242 细胞 http://www.cellresource.cn/fdetail.aspx?id=242 RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mM Hepes＋1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mM Hepes＋1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108101040.pdf 5637-cell morphology-PUMC 5637-细胞形态-PUMC Amelogenin:X；CSF1PO:11；D13S317:11；D16S539:9；D18S51:16,18；D19S433:13,15；D21S11:36；D2S1338:25；D3S1358:15,17；D5S818:11,12；D7S820:10,11；D8S1179:10,16；FGA:22TH01:7,9；TPOX:8；vWA:18 Amelogenin:X；CSF1PO:11；D13S317:11；D16S539:9；D18S51:16,18；D19S433:13,15；D21S11:36；D2S1338:25；D3S1358:15,17；D5S818:11,12；D7S820:10,11；D8S1179:10,16；FGA:22TH01:7,9；TPOX:8；vWA:18 3131C0001000700001 Human Bladder Cancer Cells; 5637（HTB-9） 人膀胱癌细胞；5637（HTB-9） epithelial cell 上皮细胞 adherent growth 贴壁生长 It is reported that the cells can produce SCF, IL-1, IL-3, IL-6, G-CSF, GM-CSF and so on. 据报道，该细胞能生成 SCF, IL-1,IL-3,IL-6,G-CSF,GM-CSF等。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A 5637-cell morpholgoy-SHCRC 5637-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion 5-10minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化5-10min。1:2。3天内可长满。 5637（HTB-9） 5637（HTB-9） cell 5637（HTB-9） 细胞 NICR572 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR572 cell NICR572 细胞 http://www.cellresource.cn/fdetail.aspx?id=572 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 5637-cell morpholgoy-SHCRC 5637-细胞形态-SHCRC Amelogenin：X，Y；CSF1PO：10，11；D13S317：10，12；D16S539：11，12；D18S51：10，12；D19S433：12，13；D21S11：30；D2S1338：19；D3S1358：16，18；D5S818：12，13；D7S820：9，11；D8S1179：8，13；FGA：20，24；TH01：9.3；TPOX：11，12；vWA：17，18； Amelogenin：X，Y；CSF1PO：10，11；D13S317：10，12；D16S539：11，12；D18S51：10，12；D19S433：12，13；D21S11：30；D2S1338：19；D3S1358：16，18；D5S818：12，13；D7S820：9，11；D8S1179：8，13；FGA：20，24；TH01：9.3；TPOX：11，12；vWA：17，18； 3111C0001CCC000346 Human Bladder Cancer Cells; J82 人膀胱癌细胞；J82 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from a 58-year-old white male with transitional cell carcinoma of the bladder. There are no desmosome in the cell, with plenty of rough endoplasmic reticulum and prominent villi, and ras gene is expressed. 该细胞源自一名58岁患有膀胱移行细胞癌的白人男性。胞内无桥粒，有大量粗面内质网及突出绒毛，表达ras基因。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P62 P62 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） Heteroploid 异倍体，XY A类 Class A J82-Cell Morphology-PUMC J82-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:10传代，每周换液2～3次 Passage at 1: 2 ~ 1:10, and change fluid for 2 to 3 times a week J82 J82 cell J82 细胞 NICR1535 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1535 cell NICR1535 细胞 http://www.cellresource.cn/fdetail.aspx?id=1535 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf J82-Cell Morphology-PUMC J82-细胞形态-PUMC 3111C0001CCC000261 Human Bladder Squamous Carcinoma Cells; SCaBER 人膀胱鳞癌细胞；SCaBER epithelioid 上皮样 adherent growth 贴壁生长 It is derived from a 58-year-old white male with squamous cell carcinoma of the bladder, containing uncommon isozymes of G6PD type A. 源于一位58岁患有膀胱鳞癌的白人男性患者，含有不常见的G6PD A型同工酶。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA,1mM sodium pyruvate MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA,1mM sodium pyruvate http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 54~58 54~58 A类 Class A SCaBER-cell morphology-PUMC SCaBER-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；2~3天换液1次。 Passage at 1: 3 ~ 1: 6; and change fluid once in 2~3 days. NICR261 SCaBER SCaBER cell SCaBER 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR261 cell NICR261 细胞 http://www.cellresource.cn/fdetail.aspx?id=261 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA,1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA,1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108100951.pdf SCaBER-cell morphology-PUMC SCaBER-细胞形态-PUMC 3131C0001000700076 Human Bladder Squamous Carcinoma Cells; SCaBER 人膀胱鳞癌细胞；SCaBER epithelial cell 上皮细胞 adherent growth 贴壁生长 It contains uncommon G6PD type A isozymes. 含有不常见的G6PD A型同工酶。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A LDH检测报告SCaBER-Isoenzyme-SHCRC LDH检测报告SCaBER-同工酶-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR644 SCaBER SCaBER cell SCaBER 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR644 cell NICR644 细胞 http://www.cellresource.cn/fdetail.aspx?id=644 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf LDH检测报告SCaBER-Isoenzyme-SHCRC LDH检测报告SCaBER-同工酶-SHCRC 3131C0001000700169 Human Bladder Epithelial Immortalized Cells; SV-HUC-1 人膀胱上皮永生化细胞；SV-HUC-1 epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell line was constructed from normal bladder tissue transfected with SV40 virus. The production of infectious SV40 was tested repeatedly with plate test of African green monkey kidney cell, and the results were all negative. The virus may be activated under stress (e.g., exposure to chemical reagents). ATCC also has a chemically transformed tumorigenic cell line, MC-SV-HUC T2, with the catalogue number of CRL-9519. 这株细胞是正常膀胱组织用SV40病毒转染建株的。 反复用非洲绿猴肾细胞平板测试检测传染性SV40的生成，结果都呈阴性。 胁迫（如曝露于化学试剂）下可能激活病毒。 ATCC还有一株化学转化的成瘤细胞株，MC-SV-HUC T2，目录号为CRL-9519。 F12K qualified fetal bovine serum，10% F12K 优质胎牛血清，10% F12K 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf P(30+5) P(30+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A SV-HUC-1-cell morphology-SHCRC SV-HUC-1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1681 SV-HUC-1 SV-HUC-1 cell SV-HUC-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1681 cell NICR1681 细胞 http://www.cellresource.cn/fdetail.aspx?id=1681 F12K 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf SV-HUC-1-cell morphology-SHCRC SV-HUC-1-细胞形态-SHCRC 3131C0001001200024 Human Bladder Transitional Cell Carcinoma; J82 人膀胱移行细胞癌；J82 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from a 58-year-old white male with transitional cell carcinoma of the bladder. There are no desmosome in the cell, with plenty of rough endoplasmic reticulum and prominent villi, and ras gene is expressed. 该细胞源自一名58岁患有膀胱移行细胞癌的白人男性。胞内无桥粒，有大量粗面内质网及突出绒毛，表达ras基因。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B J82-cell morphology-SHCRC J82-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2～1:10传代，每周换液2～3次 Passage at 1: 2 ~ 1:10, and change fluid for 2 to 3 times a week J82 J82 cell J82 细胞 NICR1605 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1605 cell NICR1605 细胞 http://www.cellresource.cn/fdetail.aspx?id=1605 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf J82-cell morphology-SHCRC J82-细胞形态-SHCRC 3131C0001000700218 Human Bladder Transitional Cell Carcinoma; J82 人膀胱移行细胞癌；J82 epithelial cell 上皮细胞 adherent growth 贴壁生长 No desmosomes were observed under the electron microscope but different numbers of rough endoplasmic reticulum and protruding microfilaments were observed. Ras (H-ras) Oncogene was included. 电子显微镜下未观察到桥粒但观察到数目不同的粗面内质网和突出微丝。 含ras (H-ras)癌基因。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(54+5) P(54+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A J82-cell morphology-SHCRC J82-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 J82 J82 cell J82 细胞 NICR1719 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1719 cell NICR1719 细胞 http://www.cellresource.cn/fdetail.aspx?id=1719 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf J82-cell morphology-SHCRC J82-细胞形态-SHCRC 3131C0001001200025 Human Bladder Transitional Cell Carcinoma; SW 780 [SW-780, SW780] 人膀胱移行细胞癌；SW 780 [SW-780, SW780] epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 1974年A. Leibovitz从第一期移行细胞瘤中建立了SW细胞株。 患者接受过术前化疗(Thiotepa)。 报道称此细胞的植板率为41%。 In 1974, A. Leibovitz established the SW cell line from the first phase of transitional cell carcinoma. The patients underwent preoperative chemotherapy (Terotepa). It was reported that the cell plate formation rate is 41%. L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B SW780-cell morphology-SHCRC SW780-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1606 SW 780 SW 780 cell SW 780 细胞 SW-780 SW-780 cell SW-780 细胞 SW780 SW780 cell SW780 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1606 cell NICR1606 细胞 http://www.cellresource.cn/fdetail.aspx?id=1606 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf SW780-cell morphology-SHCRC SW780-细胞形态-SHCRC 3131C0001000700219 Human Bladder Transitional Cell Carcinoma; SW 780 [SW-780, SW780] 人膀胱移行细胞癌；SW 780 [SW-780, SW780] epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 1974年A. Leibovitz从第一期移行细胞瘤中建立了SW细胞株。 患者接受过术前化疗(Thiotepa)。 报道称此细胞的植板率为41%。 In 1974, A. Leibovitz established the SW cell line from the first phase of transitional cell carcinoma. The patients underwent preoperative chemotherapy (Terotepa). It was reported that the cell plate formation rate is 41%. L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P(162+5) P(162+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B SW780-cell morphology-SHCRC SW780-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR2101 SW 780 SW 780 cell SW 780 细胞 SW-780 SW-780 cell SW-780 细胞 SW780 SW780 cell SW780 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2101 cell NICR2101 细胞 http://www.cellresource.cn/fdetail.aspx?id=2101 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf SW780-cell morphology-SHCRC SW780-细胞形态-SHCRC 3131C0001001200023 Human Bladder Transitional Cell Carcinoma; UM-UC-3 人膀胱移行细胞癌；UM-UC-3 epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B UM-UC-3-cell morphology-SHCRC UM-UC-3-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1604 UM-UC-3 UM-UC-3 cell UM-UC-3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1604 cell NICR1604 细胞 http://www.cellresource.cn/fdetail.aspx?id=1604 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf UM-UC-3-cell morphology-SHCRC UM-UC-3-细胞形态-SHCRC 3131C0001000700217 Human Bladder Transitional Cell Carcinoma; UM-UC-3 人膀胱移行细胞癌；UM-UC-3 epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(127+5) P(127+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B UM-UC-3-cell morphology-SHCRC UM-UC-3-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR2100 UM-UC-3 UM-UC-3 cell UM-UC-3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2100 cell NICR2100 细胞 http://www.cellresource.cn/fdetail.aspx?id=2100 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf UM-UC-3-cell morphology-SHCRC UM-UC-3-细胞形态-SHCRC Amelogenin：X；CSF1PO：10，12；D13S317：12；D16S539：9；D18S51：16，18；D19S433：13，14；D21S11：29；D2S1338：20，23；D3S1358：16；D5S818：10，12；D7S820：10，11；D8S1179：14；FGA：17，22；TH01：6；TPOX：8，11；vWA：17； Amelogenin：X；CSF1PO：10，12；D13S317：12；D16S539：9；D18S51：16，18；D19S433：13，14；D21S11：29；D2S1338：20，23；D3S1358：16；D5S818：10，12；D7S820：10，11；D8S1179：14；FGA：17，22；TH01：6；TPOX：8，11；vWA：17； 3111C0001CCC000295 Human Bladder Transitional Cell Carcinoma Cells; T24 人膀胱移行细胞癌细胞；T24 epithelioid 上皮样 adherent growth 贴壁生长 The cells were derived from the bladder transitional cell carcinoma tissue of a 81-year-old white female patient; leukemia and plasma derived from the transitional cell carcinoma patient were cytotoxic to T24 and related cell lines; the doubling time is 19 hours; Ras (H-ras) oncogene is contained and tumor-specific antigen is expressed. 该细胞源自一位81岁白人女性患者的膀胱移行细胞癌组织；来源于移行细胞癌病人的白血病和血浆对T24和相关细胞株有细胞毒性；倍增时间为19小时；含ras (H-ras)癌基因,表达肿瘤特有抗原。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A T24-Species Identification-PUMC T24-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:8传代，每周换液2~3次。 Passage at 1: 3 ~ 1: 8, and change fluid for 2~3 times a week. NICR295 T24 T24 cell T24 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR295 cell NICR295 细胞 http://www.cellresource.cn/fdetail.aspx?id=295 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf T24-Species Identification-PUMC T24-物种鉴定-PUMC Amelogenin:X；CSF1PO:10,12；D13S317:12；D16S539:9；D18S51:16,18；D19S433:14；D21S11:29；D2S1338:20,23；D3S1358:16；D5S818:10,12；D7S820:10,11；D8S1179:14；FGA:17,22；TH01:6；TPOX:8,11；vWA:17 Amelogenin:X；CSF1PO:10,12；D13S317:12；D16S539:9；D18S51:16,18；D19S433:14；D21S11:29；D2S1338:20,23；D3S1358:16；D5S818:10,12；D7S820:10,11；D8S1179:14；FGA:17,22；TH01:6；TPOX:8,11；vWA:17 3131C0001000700055 Human Bladder Transitional Cell Carcinoma Cells; T24 人膀胱移行细胞癌细胞；T24 epithelial cell 上皮细胞 adherent growth 贴壁生长 It is derived from metastatic adenocarcinoma of patients, and is cytotoxic to T24 and related cell lines. The generation time is 19 hours. It contains ras (H-ras) Oncogene. 源自病人的转移性细胞腺癌，对T24和相关细胞株有细胞毒性。 代时为19小时。 含ras (H-ras)癌基因。 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A LDH检测报告T24-Isoenzyme-SHCRC LDH检测报告T24-同工酶-SHCRC LDH检测报告T24=17-Isoenzyme-SHCRC LDH检测报告T24=17-同工酶-SHCRC T24-cell morphology-SHCRC T24-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR624 T24 T24 cell T24 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR624 cell NICR624 细胞 http://www.cellresource.cn/fdetail.aspx?id=624 RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf LDH检测报告T24-Isoenzyme-SHCRC LDH检测报告T24-同工酶-SHCRC LDH检测报告T24=17-Isoenzyme-SHCRC LDH检测报告T24=17-同工酶-SHCRC T24-cell morphology-SHCRC T24-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000076 Human Bladder Transitional Cell Carcinoma Cells; T24 人膀胱移行细胞癌细胞；T24 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were derived from the bladder transitional cell carcinoma tissue of a 81-year-old white female patient; leukemia and plasma derived from the transitional cell carcinoma patient were cytotoxic to T24 and related cell lines; the doubling time is 19 hours; Ras (H-ras) oncogene is contained and tumor-specific antigen is expressed. 该细胞源自一位81岁白人女性患者的膀胱移行细胞癌组织；来源于移行细胞癌病人的白血病和血浆对T24和相关细胞株有细胞毒性；倍增时间为19小时；含ras (H-ras)癌基因,表达肿瘤特有抗原。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C62 C62 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR844 T24 T24 cell T24 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR844 cell NICR844 细胞 http://www.cellresource.cn/fdetail.aspx?id=844 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001001200015 Human Bladder Transitional Cell Papilloma Cells; RT4 人膀胱移行细胞乳头瘤细胞；RT4 polygonal 多角 adherent growth 贴壁生长 The cells are derived from a 63-year-old white male transitional cell papilloma and can be used as a transfected host. 该细胞来源于一名63岁白人男性的移行细胞乳头状瘤，可作为转染宿主。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B RT4-cell morphology-SHCRC RT4-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1：2~1:4传代，每周2~3次。 Passage at 1: 2 ~ 1: 4, and passage for 2 to 3 times per week. NICR1596 RT4 RT4 cell RT4 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1596 cell NICR1596 细胞 http://www.cellresource.cn/fdetail.aspx?id=1596 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf RT4-cell morphology-SHCRC RT4-细胞形态-SHCRC 3131C0001000700225 Human Bladder Transitional Cell Papilloma Cells; RT4 人膀胱移行细胞乳头瘤细胞；RT4 polygonal 多角 adherent growth 贴壁生长 The cells are derived from a 63-year-old white male transitional cell papilloma and can be used as a transfected host. 该细胞来源于一名63岁白人男性的移行细胞乳头状瘤，可作为转染宿主。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf P(175+5) P(175+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B RT4-cell morphology-SHCRC RT4-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1：2~1:4传代，每周2~3次。 Passage at 1: 2 ~ 1: 4, and passage for 2 to 3 times per week. NICR2104 RT4 RT4 cell RT4 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2104 cell NICR2104 细胞 http://www.cellresource.cn/fdetail.aspx?id=2104 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf RT4-cell morphology-SHCRC RT4-细胞形态-SHCRC 3131C0001000200032 Human Embryonic Lung Fibroblast; IMR-90 人胚肺成纤维细胞；IMR-90 fibroblasts 成纤维细胞 adherent growth 贴壁生长 W.W. Nichols and his colleagues established a human diploid fibroblast cell line IMR-90 from the lungs of a 16-week-old girl. [22381] The mitotic potential, viral susceptibility and other properties have been well studied so that this cell line can be used as a replacement for WI-38 or other standard human lung cell lines. It is reported that the cell can be doubled for 58 times before aging. [22381] W.W. Nichols及其同事从一位16周女婴的肺中取材，建立了人二倍体成纤维细胞株IMR-90。 [22381] 分裂潜能，病毒感受性和其它性质都得到了充分研究，因而这株细胞可以作为WI-38或其它标准人肺细胞株的替代株。 有报道称这株细胞在表现出衰老前可倍增58次。 [22381] MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(11+5) P(11+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A IMR-90-cell morphology-SHCRC IMR-90-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 IMR-90 IMR-90 cell IMR-90 细胞 NICR1657 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1657 cell NICR1657 细胞 http://www.cellresource.cn/fdetail.aspx?id=1657 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf IMR-90-cell morphology-SHCRC IMR-90-细胞形态-SHCRC Amelogenin：X，Y；CSF1PO：11，12；D13S317：11，14；D16S539：9，11；D18S51：15，21；D19S433：14，15；D21S11：31.2；D2S1338：20；D3S1358：15，17；D5S818：11，12；D7S820：10，11；D8S1179：13；FGA：21，23；TH01：8；TPOX：8；vWA：15； Amelogenin：X，Y；CSF1PO：11，12；D13S317：11，14；D16S539：9，11；D18S51：15，21；D19S433：14，15；D21S11：31.2；D2S1338：20；D3S1358：15，17；D5S818：11，12；D7S820：10，11；D8S1179：13；FGA：21，23；TH01：8；TPOX：8；vWA：15； 3111C0001CCC000044 Human Embryonic Lung Fibroblast; MRC-5 人胚肺成纤维细胞；MRC-5 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 MRC-5细胞系来自14周龄男性胎儿的正常肺组织，该细胞老化前能传代42~46个倍增时间。 The MRC-5 cell line is derived from normal lung tissue of a 14-week-old male fetus and can be passaged for 42-46 doubling times before aging. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P20 P20 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 46，XY，partial cells are hypodiploid 46，XY，部分细胞为亚二倍体 A类 Class A MRC-5-Cell Morphology-PUMC MRC-5-Species Identification-PUMC MRC-5-cell morphology-PUMC MRC-5-物种鉴定-PUMC MRC-5-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:5传代；每周1~2次。　 Passage at 1: 2 ~ 1: 5; and passage for 1 ~ 2 times a week. MRC-5 MRC-5 cell MRC-5 细胞 NICR44 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR44 cell NICR44 细胞 http://www.cellresource.cn/fdetail.aspx?id=44 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MRC-5-Cell Morphology-PUMC MRC-5-Species Identification-PUMC MRC-5-cell morphology-PUMC MRC-5-物种鉴定-PUMC MRC-5-细胞形态-PUMC MRC-5-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:11,12；D13S317:11,14；D16S539:9,11；D18S51:15,21；D21S11:31.2；D3S1358:15,17；D5S818:11,12；D7S820:10,11；D8S1179:13；FGA:21,23；PentaD:12.；PentaE:12,16；TH01:8；TPOX:8；vWA:15 Amelogenin:X,Y；CSF1PO:11,12；D13S317:11,14；D16S539:9,11；D18S51:15,21；D21S11:31.2；D3S1358:15,17；D5S818:11,12；D7S820:10,11；D8S1179:13；FGA:21,23；PentaD:12.；PentaE:12,16；TH01:8；TPOX:8；vWA:15 3111C0002000000002 Human Embryonic Lung Fibroblast; MRC-5 人胚肺成纤维细胞；MRC-5 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 MRC-5细胞系来自14周龄男性胎儿的正常肺组织，该细胞老化前能传代42~46个倍增时间。 The MRC-5 cell line is derived from normal lung tissue of a 14-week-old male fetus and can be passaged for 42-46 doubling times before aging. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) new-born calf serum，10%；lactoalbumin hydrolysate，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10%；水解乳蛋白，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10%；水解乳蛋白，10% http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P33 P33 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） Human B,NP,G6PG,MD,AST 人B,NP,G6PG,MD,AST A类 Class A MRC-5-cell morphology-NIFDC MRC-5-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1：2-1：4 Passage at 1: 2-1: 4 MRC-5 MRC-5 cell MRC-5 细胞 NICR374 NICR374 cell NICR374 细胞 http://www.cellresource.cn/fdetail.aspx?id=374 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10%；水解乳蛋白，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10%；水解乳蛋白，10% URI: http://www.cellresource.cn/pdf/20080108100951.pdf MRC-5-cell morphology-NIFDC MRC-5-细胞形态-NIFDC 3131C0001000200012 Human Embryonic Lung Fibroblast; MRC-5 人胚肺成纤维细胞；MRC-5 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 MRC-5细胞系来自14周龄男性胎儿的正常肺组织，该细胞老化前能传代42到46次群体倍增。 它是正常二倍体细胞系，46，XY核型。 模式染色体数为46，概率为70%。此细胞系为2003年6月引进 The MRC-5 cell line is derived from normal lung tissue of a 14-week-old male fetus and can be passaged between 42 and 46 population doubling before aging. It is a normal diploid cell line, 46, XY karyotype. The typical chromosome number is 46 and the probability is 70%. This cell line was introduced in June 2003. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P（16+5） P（16+5） Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 MRC-5 MRC-5 cell MRC-5 细胞 NICR471 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR471 cell NICR471 细胞 http://www.cellresource.cn/fdetail.aspx?id=471 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3142C0001000000032 Human Embryonic Lung Fibroblast; MRC-5 人胚肺成纤维细胞；MRC-5 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 MRC-5细胞系来自14周龄男性胎儿的正常肺组织，该细胞老化前能传代42~46个倍增时间。 The MRC-5 cell line is derived from normal lung tissue of a 14-week-old male fetus and can be passaged for 42-46 doubling times before aging. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C27 C27 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. MRC-5 MRC-5 cell MRC-5 细胞 NICR800 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR800 cell NICR800 细胞 http://www.cellresource.cn/fdetail.aspx?id=800 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin：XY；D8S1179：13；D21S11：31.2；D7S820：10,11；CSF1PO：11,12；D3S1358：15,17；TH01：8；D13S317：11, 14；D16S539：9, 11；D2S1338：20；D19S433：14,15；vWA：15；THOX：8；D18S51：15,21；D5S818：11, 12；FGA：21,23。 Amelogenin：XY；D8S1179：13；D21S11：31.2；D7S820：10,11；CSF1PO：11,12；D3S1358：15,17；TH01：8；D13S317：11, 14；D16S539：9, 11；D2S1338：20；D19S433：14,15；vWA：15；THOX：8；D18S51：15,21；D5S818：11, 12；FGA：21,23。 3153C0001000000002 Human Embryonic Lung Fibroblast; MRC-5 人胚肺成纤维细胞；MRC-5 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 This cell line is from normal lung tissue of a 14-week-old male fetus. It was established by Jacobs JP in 1966. 该细胞系来自14周龄男性胎儿的正常肺组织。由 Jacobs JP于1966年建立。 M-199: with Earle＇s Balanced Salts Solution (EBSS) 10%FBS M-199: with Earle＇s Balanced Salts Solution (EBSS) 10%FBS http://www.cellresource.cn/pdf/20080108100853.pdf http://www.cellresource.cn/pdf/20080108100853.pdf PN15 PN15 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） A类 Class A MRC-5-cell morphology-KCB MRC-5-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1:2传代；3-4天一次 Passage at 1: 2; and passage once in 3-4 days MRC-5 MRC-5 cell MRC-5 细胞 NICR1276 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1276 cell NICR1276 细胞 http://www.cellresource.cn/fdetail.aspx?id=1276 M-199: with Earle＇s Balanced Salts Solution (EBSS) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100853.pdf M-199: with Earle＇s Balanced Salts Solution (EBSS) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100853.pdf MRC-5-cell morphology-KCB MRC-5-细胞形态-KCB 3131C0001000200041 Human Embryonic Lung Fibroblast; MRC-5 人胚肺成纤维细胞；MRC-5 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 MRC-5细胞系来自14周龄男性胎儿的正常肺组织，该细胞老化前能传代42到46次群体倍增。 它是正常二倍体细胞系，46，XY核型。 模式染色体数为46，概率为70%。此细胞系为2010年1月引进 The MRC-5 cell line is derived from normal lung tissue of a 14-week-old male fetus and can be passaged for 42 and 46 population doubling before aging. It is a normal diploid cell line, 46, XY karyotype. The typical chromosome number is 46 and the probability is 70%. This cell line was introduced in January 2010. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(15+5) P(15+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 MRC-5 MRC-5 cell MRC-5 细胞 NICR2089 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2089 cell NICR2089 细胞 http://www.cellresource.cn/fdetail.aspx?id=2089 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin：X；CSF1PO：10，12；D13S317：11；D16S539：11，12；D18S51：16，18；D19S433：13，16.2；D21S11：30，30.2；D2S1338：19，25；D3S1358：16，17；D5S818：10；D7S820：9，11；D8S1179：14；FGA：22，24；TH01：8，9.3；TPOX：8；vWA：19，20； Amelogenin：X；CSF1PO：10，12；D13S317：11；D16S539：11，12；D18S51：16，18；D19S433：13，16.2；D21S11：30，30.2；D2S1338：19，25；D3S1358：16，17；D5S818：10；D7S820：9，11；D8S1179：14；FGA：22，24；TH01：8，9.3；TPOX：8；vWA：19，20； 3111C0001CCC000210 Human Embryonic Lung Fibroblast; WI-38 [WI38] 人胚肺成纤维细胞；WI-38 [WI38] fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 Leonard Hayflick constructed the cell line; the cell line is limited passage cell line; life span is 50 ± 10 passages (doubling time is 24 h); normal embryonic lung tissue from gestation of 3 months. This cell line is the first human diploid cell to be used for human vaccine preparation; the addition of TNFα in the medium can accelerate cell growth. Leonard Hayflick建系；有限传代细胞系；寿命为50±10代（倍增时间24h）；来自妊娠3个月的正常胚胎肺组织。该细胞系是第一个用于人疫苗制备的人二倍体细胞；培养基中添加TNFα可以加快细胞生长。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P19 P19 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A WI-38-Species Identification-PUMC WI-38-物种鉴定-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；2~3天换液1次。 Passage at 1: 2 ~ 1: 4; and change fluid once in 2~3 days. NICR210 WI-38 WI-38 cell WI-38 细胞 WI38 WI38 cell WI38 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR210 cell NICR210 细胞 http://www.cellresource.cn/fdetail.aspx?id=210 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA URI: http://www.cellresource.cn/pdf/20080108100951.pdf WI-38-Species Identification-PUMC WI-38-物种鉴定-PUMC 3111C0002000000067 Human Embryonic Lung Fibroblast; WI-38 [WI38] 人胚肺成纤维细胞；WI-38 [WI38] fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 Leonard Hayflick constructed the cell line; the cell line is limited passage cell line; life span is 50 ± 10 passages (doubling time is 24 h); normal embryonic lung tissue from gestation of 3 months. This cell line is the first human diploid cell to be used for human vaccine preparation; the addition of TNFα in the medium can accelerate cell growth. Leonard Hayflick建系；有限传代细胞系；寿命为50±10代（倍增时间24h）；来自妊娠3个月的正常胚胎肺组织。该细胞系是第一个用于人疫苗制备的人二倍体细胞；培养基中添加TNFα可以加快细胞生长。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf Unknown 不详 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A WI-38-cell morphology-NIFDC WI-38-细胞形态-NIFDC National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR439 WI-38 WI-38 cell WI-38 细胞 WI38 WI38 cell WI38 细胞 NICR439 cell NICR439 细胞 http://www.cellresource.cn/fdetail.aspx?id=439 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf WI-38-cell morphology-NIFDC WI-38-细胞形态-NIFDC 3131C0001000200008 Human Embryonic Lung Fibroblast; WI-38 [WI38] 人胚肺成纤维细胞；WI-38 [WI38] fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 Leonard Hayflick constructed the cell line; the cell line is limited passage cell line; life span is 50 ± 10 passages (doubling time is 24 h); normal embryonic lung tissue from gestation of 3 months. This cell line is the first human diploid cell to be used for human vaccine preparation; the addition of TNFα in the medium can accelerate cell growth. Leonard Hayflick建系；有限传代细胞系；寿命为50±10代（倍增时间24h）；来自妊娠3个月的正常胚胎肺组织。该细胞系是第一个用于人疫苗制备的人二倍体细胞；培养基中添加TNFα可以加快细胞生长。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P14 P14 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR467 WI-38 WI-38 cell WI-38 细胞 WI38 WI38 cell WI38 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR467 cell NICR467 细胞 http://www.cellresource.cn/fdetail.aspx?id=467 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3142C0001000000016 Human Embryonic Lung Fibroblast; WI-38 [WI38] 人胚肺成纤维细胞；WI-38 [WI38] fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 Leonard Hayflick constructed the cell line; the cell line is limited passage cell line; life span is 50 ± 10 passages (doubling time is 24 h); normal embryonic lung tissue from gestation of 3 months. This cell line is the first human diploid cell to be used for human vaccine preparation; the addition of TNFα in the medium can accelerate cell growth. Leonard Hayflick建系；有限传代细胞系；寿命为50±10代（倍增时间24h）；来自妊娠3个月的正常胚胎肺组织。该细胞系是第一个用于人疫苗制备的人二倍体细胞；培养基中添加TNFα可以加快细胞生长。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C25 C25 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR784 WI-38 WI-38 cell WI-38 细胞 WI38 WI38 cell WI38 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR784 cell NICR784 细胞 http://www.cellresource.cn/fdetail.aspx?id=784 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf STR Identification STR鉴定 3142C0001000000095 Human Embryonic Lung Cells VA13 Cells; WI-38 人胚肺细胞VA13细胞；WI-38 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 The cell line is derived from WI-38 cells. WI-38 cells were infected with low titer SV40 virus. After continuous passage and clonal selection, several sublines were obtained, in which 2RA sublines contained SV40 neo (T) and transfer antigen, to change the characteristics of their finite cell lines, and can be continuously passaged under conventional conditions. Experimental manipulation of this cell line is very rare under biosafety level P2 conditions. 该细胞系衍生自WI-38细胞。WI-38细胞感染低滴度的SV40病毒，经过连续传代、克隆选择获得多株亚系，其中2RA亚系含有SV40 neo (T)和转移抗原，从而改变其有限细胞系的特性，可在常规条件下连续传代。该细胞系的实验操作甚少在生物安全P2级条件下进行。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C265 C265 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR863 WI-38 WI-38 cell WI-38 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR863 cell NICR863 细胞 http://www.cellresource.cn/fdetail.aspx?id=863 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin:X；CSF1PO:11,12；D13S317:12,14；D16S539:9,13；D18S51:17,18；D21S11:28,30.2；D3S1358:15,16,17；D5S818:8,9；D7S820:11；D8S1179:12,14；FGA:23；PentaD:9,10；PentaE:7,15；TH01:7,9.3；TPOX:11；VWA:16,19 Amelogenin:X；CSF1PO:11,12；D13S317:12,14；D16S539:9,13；D18S51:17,18；D21S11:28,30.2；D3S1358:15,16,17；D5S818:8,9；D7S820:11；D8S1179:12,14；FGA:23；PentaD:9,10；PentaE:7,15；TH01:7,9.3；TPOX:11；VWA:16,19 3111C0002000000112 Human Embryonic Kidney Cells; 293T 人胚肾细胞；293T epithelioid 上皮样 adherent 贴壁 This cell is derived from 293 tsA1609neo and contains SV40 large T antigen for retroviral production, gene expression and protein production. 该细胞由293tsA1609neo衍生而来，含有SV40大T抗原，用于逆转录病毒生产、基因表达和蛋白生产。 10%FBS，2mM L-glutamine,1% Penicillin/Streptomycin. 10%FBS，2mM L-glutamine,1% Penicillin/Streptomycin. 90%FBS+10%DMSO 90%FBS+10%DMSO Culture Method（-）Indicator cell Method（-） 培养法（-）指示细胞法（-） A类 Class A 293T-cell morphology-NIFDC 293T-细胞形态-NIFDC National Institute for Food and Drug Control 中国药品生物制品检定所 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 1:3-1:8，每周换液2-3次 Passage at 1: 3-1: 8, and change fluid for 2-3 times a week 293T 293T cell 293T 细胞 NICR2182 NICR2182 cell NICR2182 细胞 http://www.cellresource.cn/fdetail.aspx?id=2182 293T-cell morphology-NIFDC 293T-细胞形态-NIFDC 3131C0001000200026 Human Embryonic Kidney Cells; 2V6.11 人胚肾细胞；2V6.11 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line was derived from human embryonic kidney cell line 293 in 2001. 293 cells were transfected with pVgRXR plasmid carrying the Zeocin-resistance marker to obtain 293pVgRXR cells. Subsequently, plasmid pEKORF6 containing the Ad5 E4 ORF6 gene encoding E4 24K was transfected. PEKORF6 is derived from pIndHydro plasmid containing the hygromycin resistance gene. The vector comprises an SV40 viral sequence. The 2V6.11 cell line was initially isolated from the hygromycin-containing culture medium using a clonal ring. Human adenovirus E4 34KDa protein induced by 2V6.11 cells can be detected by immunoblotting. This cell line can be used as a tool for studying adenovirus E4 oncogene 这株细胞是2001年从人胚肾细胞株293衍化而来。293细胞用携带Zeocin-抗性标记的质粒pVgRXR转染得到293pVgRXR细胞。 随后, 用包含编码E4 24K的Ad5 E4 ORF6基因的质粒pEKORF6转染。pEKORF6从包含潮霉素抗性基因的质粒pIndHydro衍生而来。 载体包含SV40病毒序列。 2V6.11细胞株最初从含潮霉素的培养液中用克隆环分离单克隆得到。 2V6.11细胞诱导表达的人腺病毒E4 34KDa蛋白可通过免疫印迹法检测。 这株细胞可以用作研究腺病毒E4癌基因的工具 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A 2V6.11-DNA-Fingerprinting-SHCRC 2V6.11-DNA-指纹-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 2V6.11 2V6.11 cell 2V6.11 细胞 NICR483 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR483 cell NICR483 细胞 http://www.cellresource.cn/fdetail.aspx?id=483 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 2V6.11-DNA-Fingerprinting-SHCRC 2V6.11-DNA-指纹-SHCRC 3131C0001000200044 Human Embryonic Kidney Cells (SV40T Gene Modification); 293T/17 人胚肾细胞（SV40T基因修饰）；293T/17 epithelial cell 上皮细胞 adherent growth 贴壁生长 293细胞株插入SV40 T-抗原的温度敏感基因后产生的高转染效率的衍生株称为293T。 A derived cell line of the high transfection efficiency produced after insertion of the temperature sensitive gene of SV40 T antigen in 293 cell lines is called 293T. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P(17+5) P(17+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A 293T-17-cell morphology-SHCRC 293T-17-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 5-15 minutes. Passage at 1: 4-1:8. The cells can be overgrowth within 3 days. 消化5-15分钟。1:4-1:8。3天内可长满。 293T/17 293T/17 cell 293T/17 细胞 NICR2091 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2091 cell NICR2091 细胞 http://www.cellresource.cn/fdetail.aspx?id=2091 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf 293T-17-cell morphology-SHCRC 293T-17-细胞形态-SHCRC 3131C0001000200033 Human Embryo, Skin, Muscle; M-7 人胚胎，皮肤，肌肉；M-7 fibroblast-like 成纤维细胞样 adherent growth 贴壁生长 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 M-7 M-7 cell M-7 细胞 NICR486 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR486 cell NICR486 细胞 http://www.cellresource.cn/fdetail.aspx?id=486 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3131C0001000200042 Human Embryonic Lung Fibroblast; WI-38 人胚胎肺成纤维细胞；WI-38 fibroblasts 成纤维细胞 adherent growth 贴壁生长 WI-38 human diploid cell line is derived from normal embryonic lung tissue from gestation of 3 months. This cell line is the first human diploid cell line to be used for human vaccine preparation. Addition of TNF alpha to culture medium can promote cell growth. WI-38人二倍体细胞系来自妊娠3个月的正常胚胎肺组织。 该细胞系是首株用于人类疫苗制备的人二倍体细胞系。 培液中添加TNF alpha可以促进细胞生长。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(15+5) P(15+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1662 WI-38 WI-38 cell WI-38 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1662 cell NICR1662 细胞 http://www.cellresource.cn/fdetail.aspx?id=1662 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3142C0001000000333 Human Cutaneous T-cell Lymphoma cells; Hut 78 人皮肤T淋巴瘤细胞；Hut 78 lymphoblast-like 淋巴母细胞样 suspension growth 悬浮生长 HUT 78 was isolated and established in 1981 from the peripheral blood of a 53-year-old white male with Cedar's syndrome by A.F. Gazdar. This cell line has the induction-assisting characteristics of mature T cells. The surface of the cells can be washed with biologically active IL-2. HUT 78由A.F. Gazdar于1981年从一名53岁患有塞扎莱综合症的白人男性外周血中分离并建立。该细胞具有成熟T细胞的诱导－辅助特性。该细胞的表面能洗提出具有生物活性的IL-2。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C9 C9 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Hut 78 Hut 78 cell Hut 78 细胞 NICR1984 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1984 cell NICR1984 细胞 http://www.cellresource.cn/fdetail.aspx?id=1984 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin：X，Y；CSF1PO：12，13；D13S317：11；D16S539：11，12；D18S51：13，16；D19S433：15，16.2；D21S11：29，32.2；D2S1338：19，23；D3S1358：14，16；D5S818：12，13；D7S820：12；D8S1179：16；FGA：18，20；TH01：6；TPOX：11；vWA：16，17； Amelogenin：X，Y；CSF1PO：12，13；D13S317：11；D16S539：11，12；D18S51：13，16；D19S433：15，16.2；D21S11：29，32.2；D2S1338：19，23；D3S1358：14，16；D5S818：12，13；D7S820：12；D8S1179：16；FGA：18，20；TH01：6；TPOX：11；vWA：16，17； 3111C0001CCC000052 Human Skin Melanoma Cells; SK-MEL-1 人皮肤黑色素瘤细胞；SK-MEL-1 spherical 球形 suspension growth 悬浮生长 The cells were isolated and established by Oettgen F and his colleagues from the thoracic duct of a 29-year-old white male patient with extensive, fast-advancing malignant melanoma. The cells produced melanin, and the pigment particles in the cells were found to be related to their own synthesis and phagocytosis. Antibodies against this cell line were found in 63% of patients with malignant melanoma and 10% of patients with other diseases. 该细胞由Oettgen F及其同事从一名29岁的患有广泛、快速进展性恶性黑色素瘤的白人男性患者的胸导管中分离建立的。该细胞可产生黑色素，电镜检测发现细胞中色素颗粒与自身合成和吞噬作用相关。在63%的恶性黑色素瘤患者和10%其他疾病患者体内发现了针对该细胞系的抗体。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 53~73，XY 53~73，XY A类 Class A SK-MEL-1-Cell Morphology-PUMC SK-MEL-1-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com Maintain the cell concentration between 1 � 105 ~ 2 � 105, fluid replacement 2 to 3 times a week. 维持细胞浓度在1×105~2×105,每周补液2~3次。 NICR52 SK-MEL-1 SK-MEL-1 cell SK-MEL-1 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR52 cell NICR52 细胞 http://www.cellresource.cn/fdetail.aspx?id=52 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf SK-MEL-1-Cell Morphology-PUMC SK-MEL-1-细胞形态-PUMC 3111C0002000000024 Human Umbilical Vein Endothelial Cells; HUV-EC 人脐静脉内皮细胞；HUV-EC epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells can be used for the study of vascular endothelial repair injury. 该细胞可用于血管内皮修复损伤的研究。 Medium 200 + LSGS Medium 200 + LSGS P3 P3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） B类 Class B National Institute for Food and Drug Control 中国食品药品检定研究院 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net HUV-EC HUV-EC cell HUV-EC 细胞 NICR396 NICR396 cell NICR396 细胞 http://www.cellresource.cn/fdetail.aspx?id=396 3131C0001000200023 Human Umbilical Vein Endothelial Cells; HUV-EC-C 人脐静脉内皮细胞；HUV-EC-C epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells can be used for the study of vascular endothelial repair injury. 该细胞可用于血管内皮修复损伤的研究。 Medium 200 + LSGS Medium 200 + LSGS P14 P14 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn HUV-EC-C HUV-EC-C cell HUV-EC-C 细胞 NICR481 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR481 cell NICR481 细胞 http://www.cellresource.cn/fdetail.aspx?id=481 STR Identification STR鉴定 3142C0001000000138 Human Umbilical Vein Endothelial Cells; HUV-EC-C 人脐静脉内皮细胞；HUV-EC-C endothelial-like 內皮细胞样 adherent growth 贴壁生长 The cells can be used for the study of vascular endothelial repair injury. 该细胞可用于血管内皮修复损伤的研究。 F12K 10%FBS F12K 10%FBS http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf C17 C17 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HUV-EC-C HUV-EC-C cell HUV-EC-C 细胞 NICR906 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR906 cell NICR906 细胞 http://www.cellresource.cn/fdetail.aspx?id=906 F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf 3131C0001000700100 Human Prostate Cancer Cells; 22Rv1 人前列腺癌细胞；22Rv1 epithelial cell 上皮细胞 adherent growth 贴壁生长 22RV1是来自异种移植(在阉割引起前列腺癌衰退又在其父亲的雄性激素信赖型CWR22嫁接后复发的小鼠中连续传代)的人前列腺癌上皮细胞系。 此细胞系表达前列腺特异抗原。 二羟基睾丸脂酮轻微刺激细胞生长，经western blot检测溶解产物与抗雄性激素受体抗体起免疫反应。 EGF刺激细胞生长，但TGFβ-1不能抑制细胞生长。该细胞在裸鼠中成瘤。 The 22RV1 is a human prostate cancer epithelial cell line from xenografts (the xenografts was continuous passage in mice, in which the prostate cancer relapsed after castration and then recurred after transplanted with paternal androgen-dependent CWR22 cells). This cell line expressed prostate specific antigen. Dihydroxytestosterone can stimulate cell growth, and the lysate reacted with anti-androgen receptor antibody by western blot. EGF can stimulated cell growth, but TGFβ-1 did not inhibit cell growth. The cells formed tumors in nude mice. RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A 22RV1-cell morphology-SHCRC 22RV1-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 22Rv1 22Rv1 cell 22Rv1 细胞 NICR668 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR668 cell NICR668 细胞 http://www.cellresource.cn/fdetail.aspx?id=668 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf 22RV1-cell morphology-SHCRC 22RV1-cell morphology-SHCRC 22RV1-细胞形态-SHCRC 22RV1-细胞形态-SHCRC 3142C0001000000357 Human Prostate Cancer Cells; 2B4 人前列腺癌细胞；2B4 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell line is a human prostate cancer cell line and can be used for fundamental and clinical studies of human prostate cancer. 该细胞是一株人前列腺癌细胞系，可用于人前列腺癌细的基础和临床研究。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C6 C6 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. 2B4 2B4 cell 2B4 细胞 NICR2008 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2008 cell NICR2008 细胞 http://www.cellresource.cn/fdetail.aspx?id=2008 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X，Y；CSF1PO：10，11；D13S317：12，13，14；D16S539：11，13；D18S51：12；D19S433：13；D21S11：30，33；D2S1338：16；D3S1358：16；D5S818：10，13；D7S820：7，10，11；D8S1179：13，14；FGA：21，22；TH01：7；TPOX：11；vWA：17，18，19； Amelogenin：X，Y；CSF1PO：10，11；D13S317：12，13，14；D16S539：11，13；D18S51：12；D19S433：13；D21S11：30，33；D2S1338：16；D3S1358：16；D5S818：10，13；D7S820：7，10，11；D8S1179：13，14；FGA：21，22；TH01：7；TPOX：11；vWA：17，18，19； 3111C0001CCC000006 Human Prostate Cancer Cells; DU 145 [DU145; DU-145] 人前列腺癌细胞；DU 145 [DU145；DU-145] epithelioid 上皮样 adherent growth 贴壁生长 DU 145 was established from a brain metastasis of a prostate cancer patient with a 3-year history of lymphoblastic leukemia. This cell line is hormone sensitivity in dedection, and test for acid phosphatase is positive, and individual cells can form colonies in soft agar. Microvilli, microfilaments, desmosomes, mitochondria, developed Golgi apparatus and heterolysosomes can be seen in the submicroscopic structure analysis of these cells and primitive tumors. This cell does not express prostate antigen. DU 145 是从一位有3年淋巴细胞白血病史的前列腺癌患者的脑部转移灶中建立的。该细胞系未检测到激素敏感性，酸性磷酸酶阳性，单个的细胞可在软琼脂中形成集落。对此细胞和原始肿瘤的亚显微结构分析可见微绒毛、微丝、细胞桥粒、线粒体、发达的高尔基体和异质溶酶体。该细胞不表达前列腺抗原。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 59~65 59~65 A类 Class A DU145-cell morphology-PUMC DU145-细胞形态-PUMC Du145-Cell Morphology-PUMC Du145-Species Identification-PUMC Du145-物种鉴定-PUMC Du145-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:6传代；每周2~3次。 Passage at 1: 4 ~ 1: 6; and passage for 2 to 3 times per week. DU 145 DU 145 cell DU 145 细胞 DU-145 DU-145 cell DU-145 细胞 DU145 DU145 cell DU145 细胞 NICR6 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR6 cell NICR6 细胞 http://www.cellresource.cn/fdetail.aspx?id=6 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf DU145-cell morphology-PUMC DU145-细胞形态-PUMC Du145-Cell Morphology-PUMC Du145-Species Identification-PUMC Du145-物种鉴定-PUMC Du145-细胞形态-PUMC Amelogenin:X,Y；CSF1PO:10,11；D13S317:12,13,14；D16S539:11,13；D18S51:12；D19S433:13；D21S11:30,33；D2S1338:16；D3S1358:16；D5S818:10,13；D7S820:7,10,11；D8S1179:13,14；FGA:22；TH01:7；TPOX:11；vWA:17,18,19 Amelogenin:X,Y；CSF1PO:10,11；D13S317:12,13,14；D16S539:11,13；D18S51:12；D19S433:13；D21S11:30,33；D2S1338:16；D3S1358:16；D5S818:10,13；D7S820:7,10,11；D8S1179:13,14；FGA:22；TH01:7；TPOX:11；vWA:17,18,19 3131C0001000700080 Human Prostate Cancer Cells; DU 145 [DU145; DU-145] 人前列腺癌细胞；DU 145 [DU145；DU-145] epithelial cell 上皮细胞 adherent growth 贴壁生长 DU 145 was established from a brain metastasis of a prostate cancer patient with a 3-year history of lymphoblastic leukemia. This cell line and the cells isolated from soft agar did not have detectable hormone sensitivity, and test for acid phosphatase is weakly positive. Microvilli, microfilaments, desmosomes, mitochondria, developed Golgi apparatus and heterolysosomes can be seen in the submicroscopic structure analysis of these cells and primitive tumors. This cell line does not express prostate antibody. DU 145 是从一位有三年淋巴细胞白血病史的前列腺癌患者的脑部转移病灶损害中建株的。 此细胞株及从软琼脂中分离到的细胞不具有可检测的激素敏感性，酸性磷酸酶呈弱阳性。 对此株及原始肿瘤细胞的亚显微结构分析可见微绒毛，微丝及细胞桥粒。 有许多线粒体，发育良好高尔基体和异质溶酶体。 该细胞不表达前列腺抗体。 F12K fetal bovine serum，10% F12K 胎牛血清，10% F12K 胎牛血清，10% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A DU145-DNA-Fingerprinting-SHCRC DU145-DNA-指纹-SHCRC Du145-cell morphology-SHCRC Du145-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 DU 145 DU 145 cell DU 145 细胞 DU-145 DU-145 cell DU-145 细胞 DU145 DU145 cell DU145 细胞 NICR648 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR648 cell NICR648 细胞 http://www.cellresource.cn/fdetail.aspx?id=648 F12K 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf DU145-DNA-Fingerprinting-SHCRC DU145-DNA-指纹-SHCRC Du145-cell morphology-SHCRC Du145-细胞形态-SHCRC Amelogenin:X,Y；CSF1PO:10,11；D13S317:12,13,14；D16S539:11,13；D18S51:12；D19S433:13；D21S11:30,33；D2S1338:16；D3S1358:16；D5S818:10,13；D7S820:7,10,11；D8S1179:13,14；FGA:22；TH01:7；TPOX:11；vWA:17,18,19 Amelogenin:X,Y；CSF1PO:10,11；D13S317:12,13,14；D16S539:11,13；D18S51:12；D19S433:13；D21S11:30,33；D2S1338:16；D3S1358:16；D5S818:10,13；D7S820:7,10,11；D8S1179:13,14；FGA:22；TH01:7；TPOX:11；vWA:17,18,19 3131C0001000700222 Human Prostate Cancer Cells; DU 145 [DU145; DU-145] 人前列腺癌细胞；DU 145 [DU145；DU-145] epithelial cell 上皮细胞 adherent growth 贴壁生长 DU 145 was established from a brain metastasis of a prostate cancer patient with a 3-year history of lymphoblastic leukemia. This cell line and the cells isolated from soft agar did not have detectable hormone sensitivity, and test for acid phosphatase is weakly positive. Microvilli, microfilaments, desmosomes, mitochondria, developed Golgi apparatus and heterolysosomes can be seen in the submicroscopic structure analysis of these cells and primitive tumors. This cell line does not express prostate antibody. DU 145 是从一位有三年淋巴细胞白血病史的前列腺癌患者的脑部转移病灶损害中建株的。 此细胞株及从软琼脂中分离到的细胞不具有可检测的激素敏感性，酸性磷酸酶呈弱阳性。 对此株及原始肿瘤细胞的亚显微结构分析可见微绒毛，微丝及细胞桥粒。 有许多线粒体，发育良好高尔基体和异质溶酶体。 该细胞不表达前列腺抗体。 F12K fetal bovine serum，10% F12K 胎牛血清，10% F12K 胎牛血清，10% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A DU145-DNA-Fingerprinting-SHCRC DU145-DNA-指纹-SHCRC Du145-cell morphology-SHCRC Du145-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 DU 145 DU 145 cell DU 145 细胞 DU-145 DU-145 cell DU-145 细胞 DU145 DU145 cell DU145 细胞 NICR2103 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2103 cell NICR2103 细胞 http://www.cellresource.cn/fdetail.aspx?id=2103 F12K 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf DU145-DNA-Fingerprinting-SHCRC DU145-DNA-指纹-SHCRC Du145-cell morphology-SHCRC Du145-细胞形态-SHCRC Amelogenin：X，Y；CSF1PO：10，11；D13S317：10，12；D16S539：11；D18S51：9，11，12；D19S433：13.2，15；D21S11：29，32.2；D2S1338：16，17；D3S1358：16；D5S818：11，12；D7S820：8.1，9.1，10.3；D8S1179：12，14；FGA：19，20；TH01：9；TPOX：8，9；vWA：16，18； Amelogenin：X，Y；CSF1PO：10，11；D13S317：10，12；D16S539：11；D18S51：9，11，12；D19S433：13.2，15；D21S11：29，32.2；D2S1338：16，17；D3S1358：16；D5S818：11，12；D7S820：8.1，9.1，10.3；D8S1179：12，14；FGA：19，20；TH01：9；TPOX：8，9；vWA：16，18； 3111C0001CCC000040 Human Prostate Cancer Cells; LNCaP 人前列腺癌细胞；LNCaP epithelium-like; spindle 上皮样；梭形 adherent growth 贴壁生长 The cells were isolated by Horoszewicz JS in 1977 from the living tissue of a 50-year-old white male patient specifically diagnosed with metastatic prostate cancer by fine needle aspiration of the left supraclavicular lymph node. The growth of the cells and the production of acid phosphatase was influenced by 5-α-dihydrotestosterone. The cells do not form uniform monolayers but grow in clusters, so they are repeatedly blown to form individual cells during passage; the cells is not firmly adherent and do not adhere to the confluent state, which would make the culture medium acid quickly; Keep the flask stationary for 48 hours after passage. If the flask is moved, most of the cells would fall off the bottle bottom. If this occurs, it is necessary to reincubate the cells for 24-48 hrs to reattach them to the wall, and then replace the medium after the cells are adherent to the wall. 该细胞由Horoszewicz JS于1977年从一名50岁的明确诊断为转移性前列腺癌的白人男性患者的左锁骨上淋巴结细针穿刺的活体组织中分离建立的。5-α-双氢睾酮可影响该细胞的生长和酸性磷酸酶的产生。该细胞不形成均一的单层而是成簇生长，所以传代的时候要反复吹打形成单个细胞；该细胞贴壁不牢，达不到汇合状态，而且会使培养基迅速变酸；传代后48h内一定要保持培养瓶静止.如果此时挪动培养瓶，会使大部分细胞从瓶底脱落。如果出现此种情况，需要重新孵育24~48h使细胞重新贴壁，细胞贴壁后可更换培养基。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P50 P50 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 72~88，XY 72~88，XY A类 Class A LNCaP-Species Identification-PUMC LNCaP-cell morphology-PUMC LNCaP-物种鉴定-PUMC LNCaP-细胞形态-PUMC LNCap-Cell Morphology-PUMC LNCap-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；5~7天1次。 Passage at 1: 3 ~ 1: 6; and passage once 5 ~ 7 days. LNCaP LNCaP cell LNCaP 细胞 NICR40 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR40 cell NICR40 细胞 http://www.cellresource.cn/fdetail.aspx?id=40 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf LNCaP-Species Identification-PUMC LNCaP-cell morphology-PUMC LNCaP-物种鉴定-PUMC LNCaP-细胞形态-PUMC LNCap-Cell Morphology-PUMC LNCap-细胞形态-PUMC Amelogenin：X；CSF1PO：11；D13S317：11；D16S539：11；D18S51：14，15；D19S433：14；D21S11：29，31.2；D2S1338：18，20；D3S1358：16；D5S818：13；D7S820：8，11；D8S1179：13；FGA：24；TH01：6，7；TPOX：8，9；vWA：17； Amelogenin：X；CSF1PO：11；D13S317：11；D16S539：11；D18S51：14，15；D19S433：14；D21S11：29，31.2；D2S1338：18，20；D3S1358：16；D5S818：13；D7S820：8，11；D8S1179：13；FGA：24；TH01：6，7；TPOX：8，9；vWA：17； 3111C0001CCC000115 Human Prostate Cancer Cells 人前列腺癌细胞?籔C-3 [PC3] epithelioid 上皮样 adherent growth, growing in clusters in soft agar, and suspension growth 贴壁生长,在软琼脂中成簇生长，并可悬浮生长 PC-3 originated from bone metastases in a 62-year-old white male patient with grade IV prostate adenocarcinoma with low levels of acid phosphatase activity and 5-α-testosterone reductase activity. PC-3源于一位62岁白人男性IV级前列腺腺癌患者的骨转移灶；有低水平的酸性磷酸酶活性和5-α-睾酮还原酶活性。 F12K 10%FBS F12K 10%FBS http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 47~68 47~68 A类 Class A PC-3-Species Identification-PUMC PC-3-cell morphology-PUMC PC-3-物种鉴定-PUMC PC-3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:3~1:6传代；每周2~3次换液。 Passage at 1: 3 ~ 1: 6; and change fluid for 2 to 3 times per week. NICR115 PC-3 PC-3 cell PC-3 细胞 PC3 PC3 cell PC3 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR115 cell NICR115 细胞 http://www.cellresource.cn/fdetail.aspx?id=115 F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf PC-3-Species Identification-PUMC PC-3-cell morphology-PUMC PC-3-物种鉴定-PUMC PC-3-细胞形态-PUMC 3131C0001000700041 Human Prostate Cancer Cells; PC-3 [PC3] 人前列腺癌细胞；PC-3 [PC3] epithelioid 上皮样 adherent growth, growing in clusters in soft agar, and suspension growth 贴壁生长,在软琼脂中成簇生长，并可悬浮生长 PC-3 originated from bone metastases in a 62-year-old white male patient with grade IV prostate adenocarcinoma with low levels of acid phosphatase activity and 5-α-testosterone reductase activity. PC-3源于一位62岁白人男性IV级前列腺腺癌患者的骨转移灶；有低水平的酸性磷酸酶活性和5-α-睾酮还原酶活性。 F12K 10%FBS F12K 10%FBS http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf PN PN Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B PC-3-DNA-Fingerprinting-SHCRC PC-3-DNA-指纹-SHCRC PC-3-cell morphology-SHCRC PC-3-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:3~1:6传代；每周2~3次换液。 Passage at 1: 3 ~ 1: 6; and change fluid for 2 to 3 times per week. NICR611 PC-3 PC-3 cell PC-3 细胞 PC3 PC3 cell PC3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR611 cell NICR611 细胞 http://www.cellresource.cn/fdetail.aspx?id=611 F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf PC-3-DNA-Fingerprinting-SHCRC PC-3-DNA-指纹-SHCRC PC-3-cell morphology-SHCRC PC-3-细胞形态-SHCRC 3131C0001000700158 Human Prostate Cancer Cells; PC-3 [PC3] 人前列腺癌细胞；PC-3 [PC3] epithelial cell 上皮细胞 adherent growth 贴壁生长 PC-3 originated from bone metastases in a 62-year-old white male patient with grade IV prostate adenocarcinoma. Both the acid phosphatase activity and the 5-α-testosterone reductase activity of the cells are low. PC-3源于一位62岁白人男性IV级前列腺腺癌患者的骨头转移灶。 细胞的酸性磷酸酶活性和5-α-睾丸激素还原酶活性都低。 F12K qualified fetal bovine serum，10% F12K 优质胎牛血清，10% F12K 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf P(16+5) P(16+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A PC-3-DNA-Fingerprinting-SHCRC PC-3-DNA-指纹-SHCRC PC-3-cell morphology-SHCRC PC-3-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR724 PC-3 PC-3 cell PC-3 细胞 PC3 PC3 cell PC3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR724 cell NICR724 细胞 http://www.cellresource.cn/fdetail.aspx?id=724 F12K 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108102725.pdf PC-3-DNA-Fingerprinting-SHCRC PC-3-DNA-指纹-SHCRC PC-3-cell morphology-SHCRC PC-3-细胞形态-SHCRC 3142C0001000000113 Human Prostate Cancer Cells; PC-3 [PC3] 人前列腺癌细胞；PC-3 [PC3] epithelial-like 上皮细胞样 adherent growth, growing in clusters in soft agar, and suspension growth 贴壁生长,在软琼脂中成簇生长，并可悬浮生长 PC-3 originated from bone metastases in a 62-year-old white male patient with grade IV prostate adenocarcinoma with low levels of acid phosphatase activity and 5-α-testosterone reductase activity. PC-3源于一位62岁白人男性IV级前列腺腺癌患者的骨转移灶；有低水平的酸性磷酸酶活性和5-α-睾酮还原酶活性。 F12K 10%FBS F12K 10%FBS http://www.cellresource.cn/pdf/20080108102725.pdf http://www.cellresource.cn/pdf/20080108102725.pdf C23 C23 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR881 PC-3 PC-3 cell PC-3 细胞 PC3 PC3 cell PC3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR881 cell NICR881 细胞 http://www.cellresource.cn/fdetail.aspx?id=881 F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf F12K 10%FBS URI: http://www.cellresource.cn/pdf/20080108102725.pdf Amelogenin:X,Y；CSF1PO:10,12；D13S317:11,12；D16S539:9；D18S51:13；D19S433:13；D21S11:31；D2S1338:17,25；D3S1358:14,15；D5S818:12；D7S820:9,12；D8S1179:12,13；FGA:21,26；TH01:9.3；TPOX:8,11；vWA:18,19 Amelogenin:X,Y；CSF1PO:10,12；D13S317:11,12；D16S539:9；D18S51:13；D19S433:13；D21S11:31；D2S1338:17,25；D3S1358:14,15；D5S818:12；D7S820:9,12；D8S1179:12,13；FGA:21,26；TH01:9.3；TPOX:8,11；vWA:18,19 3131C0001000700220 Human Prostate Cancer Cells; VCaP 人前列腺癌细胞；VCaP epithelial cell 上皮细胞 adherent growth 贴壁生长 1997从一位不受激素影响的前列腺癌患者脊椎转移灶中建立了这株细胞。先在小鼠中进行异种移植传代，随后进行体外培养。体内及体外都对雄性激素敏感。 This cell was established in 1997 from a spinal metastasis in a prostate cancer patient without hormonal influence. The xenografts were first carried out in mice and subsequently cultured in vitro. The cells are sensitive to androgen both in vivo and in vitro. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P(39+5) P(39+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A VCaP-cell morphology-SHCRC VCaP-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR1720 VCaP VCaP cell VCaP 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1720 cell NICR1720 细胞 http://www.cellresource.cn/fdetail.aspx?id=1720 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100710.pdf VCaP-cell morphology-SHCRC VCaP-细胞形态-SHCRC 3142C0001000000144 Human Prostate Epithelial Cells; RWPE-1 人前列腺上皮细胞；RWPE-1 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were established by Webber MM in 1996. 该细胞1996年由Webber MM建立。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR912 RWPE-1 RWPE-1 cell RWPE-1 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR912 cell NICR912 细胞 http://www.cellresource.cn/fdetail.aspx?id=912 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf 3142C0001000000145 Human Prostate Normal Cells; RWPE-2 人前列腺正常细胞；RWPE-2 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were established by Webber MM in 1996. 该细胞1996年由Webber MM建立。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C7 C7 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification Chromosome 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR913 RWPE-2 RWPE-2 cell RWPE-2 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR913 cell NICR913 细胞 http://www.cellresource.cn/fdetail.aspx?id=913 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X，Y；CSF1PO：11，12；D13S317：9，11；D16S539：13，14；D18S51：14；D19S433：13，15；D21S11：30；D2S1338：24；D3S1358：15，16；D5S818：10，11；D7S820：10，12；D8S1179：12；FGA：23，24；TH01：9，9.3；TPOX：8；vWA：16； Amelogenin：X，Y；CSF1PO：11，12；D13S317：9，11；D16S539：13，14；D18S51：14；D19S433：13，15；D21S11：30；D2S1338：24；D3S1358：15，16；D5S818：10，11；D7S820：10，12；D8S1179：12；FGA：23，24；TH01：9，9.3；TPOX：8；vWA：16； 3111C0001CCC000071 Human Choriocarcinoma Cells; JEG-3 人绒癌细胞；JEG-3 epithelioid 上皮样 adherent growth 贴壁生长 This cell is one of six clones established by Kohler et al from the Woods line of Erwin-Turner tumors and can produce hCG, placental prolactin, progesterone; this cell converts steroid precursors to estrone and estradiol. 该细胞是由Kohler等从Erwin-Turner肿瘤的 Woods系分离建立的6个克隆中一个，可产生hCG、胎盘催乳素、孕激素；该细胞可将类固醇前体转化为雌酮和雌二醇。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 62~71 62~71 A类 Class A JEG-3-Species Identification-PUMC JEG-3-cell morphology-PUMC JEG-3-物种鉴定-PUMC JEG-3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:4~1:6传代；每周换液2~3次。 Passage at 1: 4 ~ 1: 6; and change fluid for 2~3 times a week. JEG-3 JEG-3 cell JEG-3 细胞 NICR71 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR71 cell NICR71 细胞 http://www.cellresource.cn/fdetail.aspx?id=71 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf JEG-3-Species Identification-PUMC JEG-3-cell morphology-PUMC JEG-3-物种鉴定-PUMC JEG-3-细胞形态-PUMC 3131C0001000700195 Human Choriocarcinoma Cells; JEG-3 人绒毛膜癌细胞；JEG-3 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This is a hypertriploid human cell line. The typical chromosome number is 71, with the incidence of 34% and the polyploid rate of 2.6%. T (4; 11) (p15; q13), i (13q), t (10p15q), del (18) (q21), and 6 other markers are common in most cells and two other markers are visible in some cells. Large satellites are visible in one N14 and two N22. N2, N5, and N9 have four copies, while N7, N13, N18, N21, and X are single copies. A single Y chromosome can be detected by Q-band detection. 这是一株超三倍体人类细胞株。 其典型染色体数为71，发生率为34%，多倍体率为2.6%。 t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21),和6个其他标记在大多数细胞中常见，另有两个标记在一些细胞中可见。 在一个N14和两个N22中可见大卫星。 N2, N5, 和 N9 有4个拷贝，而N7, N13, N18, N21 和 X为单拷贝。 Q-带检测法可以检测到单个Y染色体。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf P(127+5) P(127+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 JEG-3 JEG-3 cell JEG-3 细胞 NICR1703 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1703 cell NICR1703 细胞 http://www.cellresource.cn/fdetail.aspx?id=1703 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 URI: http://www.cellresource.cn/pdf/20080108100951.pdf 3142C0001000000346 Human Choriocarcinoma Cells; JEG-3 人绒毛膜癌细胞；JEG-3 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell is one of six clones established by Kohler et al from the Woods line of Erwin-Turner tumors and can produce hCG, placental prolactin, progesterone; this cell converts steroid precursors to estrone and estradiol. 该细胞是由Kohler等从Erwin-Turner肿瘤的 Woods系分离建立的6个克隆中一个，可产生hCG、胎盘催乳素、孕激素；该细胞可将类固醇前体转化为雌酮和雌二醇。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C8 C8 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 Isoenzyme Identification 同工酶鉴定 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. JEG-3 JEG-3 cell JEG-3 细胞 NICR1997 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1997 cell NICR1997 细胞 http://www.cellresource.cn/fdetail.aspx?id=1997 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf STR Identification STR鉴定 3142C0001000000121 Human Papillary Ovarian Adenocarcinoma Cells; Caov-3 人乳头状卵巢腺癌细胞；Caov-3 epithelial-like 上皮细胞样 adherent growth 贴壁生长 Caov-3 cell line was produced in 1976 by J. Fogh in Memorial Sloan Kettering Cancer Center. The cells were derived from ovarian tumor tissue of a 54-year-old white female. Caov-3 cell lines and their products cannot be transferred to any third parties and their commercial benefits are belong to Memorial Sloan-Kettering Cancer Center. Caov-3细胞系1976年由纪念Sloan-Kettering癌症中心的J. Fogh建立。该细胞源自一名54岁的白人女性卵巢肿瘤组织。Caov-3细胞系及其产品不能转给第三方，其商业收益归纪念Sloan-Kettering癌症中心所有。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C30 C30 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. Caov-3 Caov-3 cell Caov-3 细胞 NICR889 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR889 cell NICR889 细胞 http://www.cellresource.cn/fdetail.aspx?id=889 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：12；D13S317：11；D16S539：11，14；D18S51：17；D19S433：15；D21S11：28，29；D2S1338：19；D3S1358：17；D5S818：12；D7S820：10；D8S1179：12；FGA：22，24；TH01：7，9.3；TPOX：11；vWA：16，17； Amelogenin：X；CSF1PO：12；D13S317：11；D16S539：11，14；D18S51：17；D19S433：15；D21S11：28，29；D2S1338：19；D3S1358：17；D5S818：12；D7S820：10；D8S1179：12；FGA：22，24；TH01：7，9.3；TPOX：11；vWA：16，17； 3111C0001CCC000335 Human Breast Cancer Cells; BT-20 人乳腺癌细胞；BT-20 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were established in 1958 by E.Y. Lasfargues and L. Ozzello, from the breast cancer tissue of a 74-year-old white woman. The cells express WNT3 and WNT78. TNF alpha inhibits the cell growth. This cell is negative for estrogen receptor dedection, but express estrogen mRNA with 5 'exons deletion. 该细胞1958年由E.Y. Lasfargues 和 L. Ozzello 建系，源自一位74岁白人女性的乳腺癌组织。该细胞表达WNT3和WNT78。TNF alpha抑制该细胞生长。该细胞雌激素受体阴性，但表达5＇外显子缺失的雌激素mRNA。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf http://www.cellresource.cn/pdf/20080108100951.pdf C3 C3 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） N3, N4, N9, N13, N14,X chromosome may be lost N3, N4, N9, N13, N14,X染色体可能丢失 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:4传代，2～3天换液一次 Passage at 1: 2 ~ 1: 4, and change fluid once in 2~3 days BT-20 BT-20 cell BT-20 细胞 NICR1524 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1524 cell NICR1524 细胞 http://www.cellresource.cn/fdetail.aspx?id=1524 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100951.pdf STR Identification STR鉴定 3142C0001000000314 Human Breast Cancer Cells; BT474 人乳腺癌细胞；BT474 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were deposited by Professor Su Li of Life Science College of Huazhong University of Science and Technology and can be used for basic research. 该细胞由华中科技大学生命科学学院苏莉教授提交保藏，可用于相关的基础研究。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C84 C84 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. BT474 BT474 cell BT474 细胞 NICR1965 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1965 cell NICR1965 细胞 http://www.cellresource.cn/fdetail.aspx?id=1965 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:10,12；D13S317:11；D16S539:8；D18S51:15；D19S433:15.2；D21S11:32.2；D2S1338:17；D3S1358:18；D5S818:11；D7S820:9,10；D8S1179:16；FGA:19；TH01:9.3；TPOX:8；vWA:15,1 Amelogenin:X；CSF1PO:10,12；D13S317:11；D16S539:8；D18S51:15；D19S433:15.2；D21S11:32.2；D2S1338:17；D3S1358:18；D5S818:11；D7S820:9,10；D8S1179:16；FGA:19；TH01:9.3；TPOX:8；vWA:15,1 3131C0001000700093 Human Breast Cancer Cells; BT-549 人乳腺癌细胞；BT-549 epithelial cell 上皮细胞 adherent growth 贴壁生长 The origin of the tissue is a papillary invasive ductal tumor, and 3 of the 7 local lymph nodes have been metastasized. The established cells are polymorphic, including epithelial cell-like components and polynuclear giant cells. Myxoid-like substances are secreted into the medium. 来源组织是一个乳头状侵入性导管瘤，7个局部淋巴结中3个已有转移。 建立的细胞是多态性的，包括上皮细胞样组分和多核巨细胞。 向培养基中分泌粘液素样物质。 RPMI 1640 (w/o Hepes) fetal bovine serum，10%；0.023IU/ml insulin RPMI 1640 (w/o Hepes) 胎牛血清，10%；0.023IU/ml胰岛素 RPMI 1640 (w/o Hepes) 胎牛血清，10%；0.023IU/ml胰岛素 http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A BT-549-DNA-Fingerprinting-SHCRC BT-549-DNA-指纹-SHCRC BT-549-cell morphology-SHCRC BT-549-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 BT-549 BT-549 cell BT-549 细胞 NICR661 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR661 cell NICR661 细胞 http://www.cellresource.cn/fdetail.aspx?id=661 RPMI 1640 (w/o Hepes) 胎牛血清，10%；0.023IU/ml胰岛素 URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 胎牛血清，10%；0.023IU/ml胰岛素 URI: http://www.cellresource.cn/pdf/20080108101040.pdf BT-549-DNA-Fingerprinting-SHCRC BT-549-DNA-指纹-SHCRC BT-549-cell morphology-SHCRC BT-549-细胞形态-SHCRC 3131C0001000700148 Human Breast Cancer Cells; HCC1937 人乳腺癌细胞；HCC1937 epithelial-like 上皮细胞样 adherent growth 贴壁生长 This cell was originally derived from primary ductal carcinoma on October 13, 1995, and was established for 11.5 months. The tumor classification was TNM IIB stage, grade III. BRCA1 analysis showed that this cell line was homozygous for the BRCA1 5382C mutation, while lymphoblast-like cell lines derived from the same patient were heterozygous at this mutation site. Two other family members also had the mutation; an identical twin also had breast cancer. This cell has an acquired TP53 mutation and its wild-type allele is lost; an acquired PTEN gene is homozygously lost; and multiple sites associated with breast cancer pathogenesis occur heterozygous mutations. Both Her2-neu and p53 expression were negative. 这株细胞1995年10月13日最初来源于原发性导管癌, 用了11.5个月建株。肿瘤分类为TNM IIB期, 3级。BRCA1分析表明这株细胞是BRCA1 5382C突变纯合的, 而来源于同一病人的类淋巴母细胞细胞株在这个突变位点上是杂合的。 另两个家庭成员也有这个突变; 一个同卵双生姐妹也患有乳腺癌。这株细胞有一个后天的TP53突变, 而其野生型等位基因丢失; 一个PTEN基因的后天的纯合缺失, 以及多个与乳腺癌发病机理相关的位点上发生的杂合突变。这株细胞Her2-neu和p53表达都呈阴性。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P(15+5) P(15+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A HCC1937-cell morphology-SHCRC HCC1937-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion 5-10 minutes. Passage at 1: 2. The cells can be overgrowth within 4-5 days. 消化5-10分钟。1:2。4-5天长满。 HCC1937 HCC1937 cell HCC1937 细胞 NICR714 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR714 cell NICR714 细胞 http://www.cellresource.cn/fdetail.aspx?id=714 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf HCC1937-cell morphology-SHCRC HCC1937-细胞形态-SHCRC Amelogenin：X,X；CSF1PO：12,12；D12S391：21,21；D13S317：13,13；D16S539：13,14；D18S51：12,12；D19S433：14,15；D21S11：28,28；D2S1338：25,25；D3S1358：18,18；D5S818：12,12；D6S1043：11,11；D7S820：9,10；D8S1179：12,13；FGA：20,22；Penta E：13,13；TH01：6,6；TPOX：11,11；vWA：16,17； Amelogenin：X,X；CSF1PO：12,12；D12S391：21,21；D13S317：13,13；D16S539：13,14；D18S51：12,12；D19S433：14,15；D21S11：28,28；D2S1338：25,25；D3S1358：18,18；D5S818：12,12；D6S1043：11,11；D7S820：9,10；D8S1179：12,13；FGA：20,22；Penta E：13,13；TH01：6,6；TPOX：11,11；vWA：16,17； 3111C0001CCC000471 Human Breast Cancer Cells; HCC1937 人乳腺癌细胞；HCC1937 epithelium-like; polygonal shape 上皮样；多角形 adherent growth 贴壁生长 This cell was originally derived from primary ductal carcinoma on October 13, 1995, and was established for 11.5 months. The tumor classification was TNM IIB stage, grade III. BRCA1 analysis showed that this cell line was homozygous for the BRCA1 5382C mutation, while lymphoblast-like cell lines derived from the same patient were heterozygous at this mutation site. Two other family members also had the mutation; an identical twin also had breast cancer. This cell has an acquired TP53 mutation and its wild-type allele is lost; an acquired PTEN gene is homozygously lost; and multiple sites associated with breast cancer pathogenesis occur heterozygous mutations. Both Her2-neu and p53 expression were negative. 这株细胞1995年10月13日最初来源于原发性导管癌, 用了11.5个月建株。肿瘤分类为TNM IIB期, 3级。BRCA1分析表明这株细胞是BRCA1 5382C突变纯合的, 而来源于同一病人的类淋巴母细胞细胞株在这个突变位点上是杂合的。 另两个家庭成员也有这个突变; 一个同卵双生姐妹也患有乳腺癌。这株细胞有一个后天的TP53突变, 而其野生型等位基因丢失; 一个PTEN基因的后天的纯合缺失, 以及多个与乳腺癌发病机理相关的位点上发生的杂合突变。这株细胞Her2-neu和p53表达都呈阴性。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代；4-5天传代一次。 Passage at 1: 2; and passage once in 4-5 days. HCC1937 HCC1937 cell HCC1937 细胞 NICR2144 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR2144 cell NICR2144 细胞 http://www.cellresource.cn/fdetail.aspx?id=2144 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:11,13；D13S317:11,13；D16S539:9,12；D18S51:16；D19S433:14,15；D21S11:29,32.2；D2S1338:17,26；D3S1358:16,17；D5S818:11,13；D7S820:10；D8S1179:13；FGA:23,24；TH01:9,9.3；TPOX:8；vWA:17 Amelogenin:X；CSF1PO:11,13；D13S317:11,13；D16S539:9,12；D18S51:16；D19S433:14,15；D21S11:29,32.2；D2S1338:17,26；D3S1358:16,17；D5S818:11,13；D7S820:10；D8S1179:13；FGA:23,24；TH01:9,9.3；TPOX:8；vWA:17 3131C0001000700127 Human Breast Cancer Cells; Hs 578T 人乳腺癌细胞；Hs 578T epithelial cell 上皮细胞 adherent growth 贴壁生长 Hs 578T cell line originated from breast cancer. It originated from the same patient as the normal fibroblast-like cell line Hs 578Bst. The Hs 578T cell line was polygonal initially, but the star-shaped cell morphology was selected and cloned during passage. The aggregation of casein granules, desmosomes, tight junctions, liposomes and smooth endoplasmic reticulum vesicles are observed under electron microscope. As with Hs 578Bst, estrogen receptor and endophytic virus are not detected. Hs 578T细胞株源自乳房癌。 它与正常成纤维细胞样细胞株Hs 578Bst起源于同一位患者。 Hs 578T细胞株最初是多角形的，但在传代过程中选择并克隆了星形的细胞形态。 电镜下观察到酪蛋白颗粒聚集，桥粒，紧密连接，脂质体和光滑内质网小泡。 与Hs 578Bst一样，未检测到雌激素受体和内生病毒。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10%；0.01mg/ml bovine insulin DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%；0.01mg/ml牛胰岛素 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%；0.01mg/ml牛胰岛素 http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf P(49+5) P(49+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 Hs 578T Hs 578T cell Hs 578T 细胞 NICR695 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR695 cell NICR695 细胞 http://www.cellresource.cn/fdetail.aspx?id=695 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%；0.01mg/ml牛胰岛素 URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%；0.01mg/ml牛胰岛素 URI: http://www.cellresource.cn/pdf/20080108100710.pdf AMEL:X,X；CSF1PO:13,13；D12S391:19,19；D13S317:11,11；D16S539:9,12；D18S51:16,16；D19S433:14,15；D21S11:29,32.2；D2S1338:17,26；D2S441:14,14；D3S1358:16,17；D5S818:11,11；D6S1043:12,12；D7S820:10,10；D8S1179:13,13；FGA:23,24；Penta D:8,13；Penta E:13,14；TH01:9,9.3；TPOX:8, AMEL:X,X；CSF1PO:13,13；D12S391:19,19；D13S317:11,11；D16S539:9,12；D18S51:16,16；D19S433:14,15；D21S11:29,32.2；D2S1338:17,26；D2S441:14,14；D3S1358:16,17；D5S818:11,11；D6S1043:12,12；D7S820:10,10；D8S1179:13,13；FGA:23,24；Penta D:8,13；Penta E:13,14；TH01:9,9.3；TPOX:8, 3142C0001000000961 Human Breast Cancer Cells; HS578T 人乳腺癌细胞；HS578T epithelial-like 上皮细胞样 adherent growth 贴壁生长 Hs 578T is of mixed polygonal morphology in which stellate cell types are selected for use in channels and clones. Hs 578T是混合多边形形态，其中星状细胞类型被选择用于在通道和克隆。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. HS578T HS578T cell HS578T 细胞 NICR2198 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2198 cell NICR2198 细胞 http://www.cellresource.cn/fdetail.aspx?id=2198 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：10；D13S317：11；D16S539：11；D18S51：16；D19S433：14.2；D21S11：31，31.2；D2S1338：25；D3S1358：16；D5S818：12；D7S820：10，11；D8S1179：12，14；FGA：23；TH01：7，8；TPOX：11；vWA：15； Amelogenin：X；CSF1PO：10；D13S317：11；D16S539：11；D18S51：16；D19S433：14.2；D21S11：31，31.2；D2S1338：25；D3S1358：16；D5S818：12；D7S820：10，11；D8S1179：12，14；FGA：23；TH01：7，8；TPOX：11；vWA：15； 3111C0001CCC000077 Human Breast Cancer Cells; MDA-MB-157 人乳腺癌细胞；MDA-MB-157 epithelioid 上皮样 adherent growth 贴壁生长 The cells are derived from a 44-year-old black woman with myeloid breast cancer and express the WNT7B oncogene, with cell desmosomes, microvilli, and tension filaments at the cell-to-cell boundaries. 该细胞源自一位患有乳腺髓样癌的44岁黑人女性，表达WNT7B癌基因，细胞与细胞边界处有细胞桥粒、微绒毛、张力细丝。 L15: Leibovitz Medium 10%FBS L15: Leibovitz Medium 10%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A MDA-MB-157-Cell Morphology-PUMC MDA-MB-157-cell morphology-PUMC MDA-MB-157-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:3传代；每周换液2~3次。 Passage at 1: 2 ~ 1: 3; and change fluid for 2~3 times a week. MDA-MB-157 MDA-MB-157 cell MDA-MB-157 细胞 NICR77 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR77 cell NICR77 细胞 http://www.cellresource.cn/fdetail.aspx?id=77 L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-157-Cell Morphology-PUMC MDA-MB-157-cell morphology-PUMC MDA-MB-157-细胞形态-PUMC MDA-MB-157-细胞形态-PUMC Amelogenin：X；CSF1PO：12，13；D13S317：13；D16S539：12；D18S51：11，16；D19S433：11，14；D21S11：30，33.2；D2S1338：20，21；D3S1358：16；D5S818：12；D7S820：8，9；D8S1179：13；FGA：22，23；TH01：7，9.3；TPOX：8，9；vWA：15，18； Amelogenin：X；CSF1PO：12，13；D13S317：13；D16S539：12；D18S51：11，16；D19S433：11，14；D21S11：30，33.2；D2S1338：20，21；D3S1358：16；D5S818：12；D7S820：8，9；D8S1179：13；FGA：22，23；TH01：7，9.3；TPOX：8，9；vWA：15，18； 3111C0001CCC000014 Human Breast Cancer Cells; MDA-MB-231 人乳腺癌细胞；MDA-MB-231 epithelioid 上皮样 adherent growth 贴壁生长 MDA-MB-231 was isolated and established from the hydrothorax of a 51-year-old white female breast cancer patient. The cells express epidermal growth factor EGF receptor, TGF-α receptor and WNT7B oncogene. MDA-MB-231是从一名51岁的白人女性乳腺癌患者的胸水中分离建立的。该细胞表达表皮生长因子EGF受体、TGF-α受体和WNT7B癌基因。 L15: Leibovitz Medium 10%FBS L15: Leibovitz Medium 10%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 58~61，there is megakaryocyte 58~61，有巨核 A类 Class A MDA-MB-231-Cell Morphology-PUMC MDA-MB-231-Species Identification-PUMC MDA-MB-231-cell morphology-PUMC MDA-MB-231-物种鉴定-PUMC MDA-MB-231-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；每周2~3次。 Passage at 1: 2 ~ 1: 4; and passage for 2 to 3 times per week. MDA-MB-231 MDA-MB-231 cell MDA-MB-231 细胞 NICR14 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR14 cell NICR14 细胞 http://www.cellresource.cn/fdetail.aspx?id=14 L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-231-Cell Morphology-PUMC MDA-MB-231-Species Identification-PUMC MDA-MB-231-cell morphology-PUMC MDA-MB-231-物种鉴定-PUMC MDA-MB-231-细胞形态-PUMC MDA-MB-231-细胞形态-PUMC Amelogenin:X；CSF1PO:12,13；D13S317:13；D16S539:12；D18S51:11,16；D19S433:11,14；D21S11:33.2；D2S1338:20,21；D3S1358:16；D5S818:12；D7S820:8,9；D8S1179:13；FGA:22,23；TH01:7,9.3；TPOX:8,9；vWA:15,18 Amelogenin:X；CSF1PO:12,13；D13S317:13；D16S539:12；D18S51:11,16；D19S433:11,14；D21S11:33.2；D2S1338:20,21；D3S1358:16；D5S818:12；D7S820:8,9；D8S1179:13；FGA:22,23；TH01:7,9.3；TPOX:8,9；vWA:15,18 3131C0001000700104 Human Breast Cancer Cells; MDA-MB-231 人乳腺癌细胞；MDA-MB-231 epithelial cell 上皮细胞 adherent growth 贴壁生长 MDA-MB-231 is derived from the hydrothorax of a 51-year-old female with metastatic breast adenocarcinoma. In nude mice and ALS treated BALB/c mice, it can form low differentiation adenocarcinoma (grade III). MDA-MB-231来自患有转移乳腺腺癌的51岁女病人的胸水。 在裸鼠和ALS处理的BALB/c小鼠中，它能形成低分化腺癌（III级）。 L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P(28+5) P(28+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A MDA-MB-231-cell morphology-SHCRC MDA-MB-231-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 MDA-MB-231 MDA-MB-231 cell MDA-MB-231 细胞 NICR672 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR672 cell NICR672 细胞 http://www.cellresource.cn/fdetail.aspx?id=672 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-231-cell morphology-SHCRC MDA-MB-231-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000323 Human Breast Cancer Cells; MDA-MB-231 人乳腺癌细胞；MDA-MB-231 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were exchanged and preserved by Professor Gong Feili, Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology. MDA-MB-231 Cells can be used for the basis and clinical study of pathogenesis of human breast cancer. 该细胞由华中科技大学同济医学院免疫系龚非力教授交换保藏。MDA-MB-231细胞可用于人乳腺癌发病机理的基础和临床研究。 L15: Leibovitz Medium 10%FBS L15: Leibovitz Medium 10%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf C27 C27 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. MDA-MB-231 MDA-MB-231 cell MDA-MB-231 细胞 NICR1974 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1974 cell NICR1974 细胞 http://www.cellresource.cn/fdetail.aspx?id=1974 L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf Amelogenin：X；CSF1PO:12,13；D13S317:13；D16S539:12；D18S51:11,16；D19S433:11,14；D21S11:33.2；D2S1338:20,21；D3S1358:15,16；D5S818:12；D7S820:8,9；D8S1179:13；FGA:22,23；TH01:7,9.3；THOX:8,9；vWA:15,18； Amelogenin：X；CSF1PO:12,13；D13S317:13；D16S539:12；D18S51:11,16；D19S433:11,14；D21S11:33.2；D2S1338:20,21；D3S1358:15,16；D5S818:12；D7S820:8,9；D8S1179:13；FGA:22,23；TH01:7,9.3；THOX:8,9；vWA:15,18； 3153C0001000000222 Human Breast Cancer Cells; MDA-MB-231 人乳腺癌细胞；MDA-MB-231 epithelioid cells 上皮样细胞 adherent growth 贴壁生长 The cell line is derived from hydrothorax from a 51-year-old female patient with metastatic breast adenocarcinoma. In nude mice and ALS treated BALB/c mice, it can form low differentiation adenocarcinoma (grade III). 该细胞系来源于患有转移乳腺腺癌的51岁女病人的胸水。 在裸鼠和ALS处理的BALB/c小鼠中，它能形成低分化腺癌（III级）。 10%FBS 10%FBS PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Fluorescence Method（-） 荧光法（-） None 无 52-68 52-68 A类 Class A MDA-MB-231-cell morphology-KCB MDA-MB-231-细胞形态-KCB Kunming Cell Bank, Chinese Academy of Sciences 中国科学院昆明细胞库 Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 1:3传代；3-4天一次 Passage at 1: 3; and passage once in 3-4 days MDA-MB-231 MDA-MB-231 cell MDA-MB-231 细胞 NICR2173 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR2173 cell NICR2173 细胞 http://www.cellresource.cn/fdetail.aspx?id=2173 MDA-MB-231-cell morphology-KCB MDA-MB-231-细胞形态-KCB Amelogenin：X；CSF1PO：12；D13S317：11；D16S539：11，12；D18S51：12，15；D19S433：12.2，14；D21S11：30，32.2；D2S1338：19，20；D3S1358：16；D5S818：10，11；D7S820：9，12；D8S1179：15；FGA：20，24；TH01：9.3；TPOX：8，11；vWA：17； Amelogenin：X；CSF1PO：12；D13S317：11；D16S539：11，12；D18S51：12，15；D19S433：12.2，14；D21S11：30，32.2；D2S1338：19，20；D3S1358：16；D5S818：10，11；D7S820：9，12；D8S1179：15；FGA：20，24；TH01：9.3；TPOX：8，11；vWA：17； 3111C0001CCC000381 Human Breast Cancer Cells; MDA-MB-361 人乳腺癌细胞；MDA-MB-361 epithelial-like 上皮细胞样 loosely adherent growth 松散贴壁生长 The cells are derived from brain metastases of 40-year-old women with breast cancer. 该细胞源自40岁女性乳腺癌的脑转移组织。 L15: Leibovitz Medium 20%FBS L15: Leibovitz Medium 20%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P56 P56 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 54～61 54～61 A类 Class A MDA-MB-361-Cell Morphology-PUMC MDA-MB-361-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:6传代，每周换液2～3次 Passage at 1: 2 ~ 1: 6, and change fluid for 2 to 3 times a week MDA-MB-361 MDA-MB-361 cell MDA-MB-361 细胞 NICR1570 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1570 cell NICR1570 细胞 http://www.cellresource.cn/fdetail.aspx?id=1570 L15: Leibovitz Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-361-Cell Morphology-PUMC MDA-MB-361-细胞形态-PUMC Amelogenin：X；CSF1PO：10，12；D13S317：11，13；D16S539：13；D18S51：13；D19S433：13，13.2；D21S11：27，28；D2S1338：19，24；D3S1358：17；D5S818：11，13；D7S820：9，10；D8S1179：8，12；FGA：22；TH01：7，9.3；TPOX：8；vWA：17； Amelogenin：X；CSF1PO：10，12；D13S317：11，13；D16S539：13；D18S51：13；D19S433：13，13.2；D21S11：27，28；D2S1338：19，24；D3S1358：17；D5S818：11，13；D7S820：9，10；D8S1179：8，12；FGA：22；TH01：7，9.3；TPOX：8；vWA：17； 3111C0001CCC000350 Human Breast Cancer Cells; MDA-MB-415 人乳腺癌细胞；MDA-MB-415 epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells are derived from a 38-year-old woman with breast adenocarcinoma and express WNT7B oncogene. 该细胞源于一名38岁患有乳腺腺癌女性的胸腔积液，细胞表达WNT7B癌基因。 L15: Leibovitz Medium 15%FBS；10mcg/ml insulin +10 mcg/ml glutathione L15: Leibovitz Medium 15%FBS；10mcg/ml insulin +10 mcg/ml glutathione http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P38 P38 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 65～76 65～76 A类 Class A MDA-MB-415-Cell Morphology-PUMC MDA-MB-415-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代，每周换液2～3次 Passage at 1: 2, and change fluid for 2 to 3 times a week MDA-MB-415 MDA-MB-415 cell MDA-MB-415 细胞 NICR1539 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1539 cell NICR1539 细胞 http://www.cellresource.cn/fdetail.aspx?id=1539 L15: Leibovitz Medium 15%FBS；10mcg/ml insulin +10 mcg/ml glutathione URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 15%FBS；10mcg/ml insulin +10 mcg/ml glutathione URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-415-Cell Morphology-PUMC MDA-MB-415-细胞形态-PUMC 3131C0001000700036 Human Breast Cancer Cells; MDA-MB-435S 人乳腺癌细胞；MDA-MB-435S spindle 纺锤形 adherent growth 贴壁生长 MDA-MB-435S is a spindle-shaped cell selected from its parent (435) in 1976. The 435 was isolated from the hydrothorax of a 31-year-old female patient with metastatic breast ductal adenocarcinoma. When tubulin is stained with a fluorescent dye, the parent cells show interspersed characteristic (Type II). Recent cDNA array studies have shown that the parent (MDA-MB-435) can be included in the origin of melanoma. MDA-MB-435S是一种纺锤形的细胞，1976年由其亲本(435)中筛选得到。435是从31岁的转移性乳腺导管腺癌女性患者胸水中分离得到。 当用荧光染料对微管蛋白进行染色时亲本细胞显现散布特征(II型)。 最近通过cDNA阵列研究表明，亲本(MDA-MB-435)可归入黑素瘤起源。 L15: Leibovitz Medium qualified fetal bovine serum，10%；0.01mg/ml insulin L15: Leibovitz Medium 优质胎牛血清，10%；0.01mg/ml胰岛素 L15: Leibovitz Medium 优质胎牛血清，10%；0.01mg/ml胰岛素 http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P(239+5) P(239+5) Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen Unit: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences Zip code: 200032 Address: No. 320, Yueyang Road, Shanghai Tel: 021-54920404，021-54920405 Fax: 021-54920583 Email: shchen@sibs.ac.cn 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920583 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 MDA-MB-435S MDA-MB-435S cell MDA-MB-435S 细胞 NICR606 1. Cailleau R, Oliv� M, Cruciger QV. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro, 1978, 14(11): 911-915. 2. Rae JM, Ramus SJ, Waltham M, et al. Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clin Exp Metastasis, 2004, 21(6): 543-552. 3. Kuo YC, Su CH, Liu CY, et al. Transforming growth factor-beta induces CD44 cleavage that promotes migration of MDA-MB-435s cells through the up-regulation of membrane type 1-matrix metalloproteinase. Int J Cancer, 2009, 124(11): 2568-2576. 1. Cailleau R, Oliv� M, Cruciger QV. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro, 1978, 14(11): 911-915. 2. Rae JM, Ramus SJ, Waltham M, et al. Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clin Exp Metastasis, 2004, 21(6): 543-552. 3. Kuo YC, Su CH, Liu CY, et al. Transforming growth factor-beta induces CD44 cleavage that promotes migration of MDA-MB-435s cells through the up-regulation of membrane type 1-matrix metalloproteinase. Int J Cancer, 2009, 124(11): 2568-2576. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR606 cell NICR606 细胞 http://www.cellresource.cn/fdetail.aspx?id=606 L15: Leibovitz Medium 优质胎牛血清，10%；0.01mg/ml胰岛素 URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10%；0.01mg/ml胰岛素 URI: http://www.cellresource.cn/pdf/20080108100839.pdf STR Identification STR鉴定 3142C0001000000052 Human Breast Cancer Cells; MDA-MB-435S 人乳腺癌细胞；MDA-MB-435S epithelial-like 上皮细胞样 adherent growth 贴壁生长 MDA-MB-435S is a spindle-shaped cell selected from its parent (MDA-MB-435) in 1976. The 435 was isolated from the hydrothorax of a 31-year-old female patient with metastatic breast ductal adenocarcinoma. But recently it has been demonstrated that MDA-MB-435 cells are contaminated with M14 melanoma cells. MDA-MB-435S是一种纺锤形的细胞，1976年由其亲本(MDA-MB-435)中筛选得到。MDA-MB-435是从一名31岁的转移性乳腺导管腺癌女性患者胸水中分离得到的。但近来证明MDA-MB-435细胞被M14黑色素瘤细胞污染。 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf http://www.cellresource.cn/pdf/20080108100710.pdf C246 C246 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. MDA-MB-435S MDA-MB-435S cell MDA-MB-435S 细胞 NICR820 1. Cailleau R, Oliv� M, Cruciger QV. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro, 1978, 14(11): 911-915. 2. Rae JM, Ramus SJ, Waltham M, et al. Common origins of MDA-MB-435 cells from various so 1. Cailleau R, Oliv� M, Cruciger QV. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro, 1978, 14(11): 911-915. 2. Rae JM, Ramus SJ, Waltham M, et al. Common origins of MDA-MB-435 cells from various so The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR820 cell NICR820 细胞 http://www.cellresource.cn/fdetail.aspx?id=820 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100710.pdf Amelogenin：X；CSF1PO：12；D13S317：10；D16S539：9，11；D18S51：12；D19S433：13；D21S11：30，31.2；D2S1338：23；D3S1358：18；D5S818：13；D7S820：10；D8S1179：10，14；FGA：21，24；TH01：9.3；TPOX：8；vWA：14，20； Amelogenin：X；CSF1PO：12；D13S317：10；D16S539：9，11；D18S51：12；D19S433：13；D21S11：30，31.2；D2S1338：23；D3S1358：18；D5S818：13；D7S820：10；D8S1179：10，14；FGA：21，24；TH01：9.3；TPOX：8；vWA：14，20； 3111C0001CCC000352 Human Breast Cancer Cells; MDA-MB-436 人乳腺癌细胞；MDA-MB-436 polygonal 多角形 adherent growth 贴壁生长 The cells were derived from pleural effusion of a 43-year-old woman with breast adenocarcinoma. 该细胞源于一名43岁患有乳腺腺癌女性的胸腔积液。 L15: Leibovitz Medium 10% FBS；10 mcg/ml insulin, 16 mcg/ml glutathione L15: Leibovitz Medium 10% FBS；10 mcg/ml insulin, 16 mcg/ml glutathione http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P23 P23 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A MDA-MB-436-Cell Morphology-PUMC MDA-MB-436-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代，每周换液2～3次 Passage at 1: 2, and change fluid for 2 to 3 times a week MDA-MB-436 MDA-MB-436 cell MDA-MB-436 细胞 NICR1541 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1541 cell NICR1541 细胞 http://www.cellresource.cn/fdetail.aspx?id=1541 L15: Leibovitz Medium 10% FBS；10 mcg/ml insulin, 16 mcg/ml glutathione URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10% FBS；10 mcg/ml insulin, 16 mcg/ml glutathione URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-436-Cell Morphology-PUMC MDA-MB-436-细胞形态-PUMC Amelogenin：X；CSF1PO：10，12；D13S317：12；D16S539：9；D18S51：15，20；D19S433：13，14；D21S11：29，31；D2S1338：23，24；D3S1358：15，OL；D5S818：11；D7S820：10；D8S1179：10，12；FGA：18，23；TH01：6；TPOX：10；vWA：17，18； Amelogenin：X；CSF1PO：10，12；D13S317：12；D16S539：9；D18S51：15，20；D19S433：13，14；D21S11：29，31；D2S1338：23，24；D3S1358：15，OL；D5S818：11；D7S820：10；D8S1179：10，12；FGA：18，23；TH01：6；TPOX：10；vWA：17，18； 3111C0001CCC000016 Human Breast Cancer Cells; MDA-MB-453 人乳腺癌细胞；MDA-MB-453 epithelium-like; polygonal shape 上皮样；多角形 adherent growth 贴壁生长 The cell line was isolated from the pericardial exudates of a 48-year-old white woman with metastatic breast cancer by Cailleau R in 1976. This cell expresses the receptor for FGF. 该细胞系由Cailleau R在1976年从一名48岁的患有转移性乳腺癌的白人女性的心包渗出液中分离建立的。该细胞表达FGF的受体。 L15: Leibovitz Medium 10%FBS L15: Leibovitz Medium 10%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 72~90 72~90 A类 Class A MDA-MB-453-Cell Morphology-PUMC MDA-MB-453-Species Identification-PUMC MDA-MB-453-cell morphology-PUMC MDA-MB-453-物种鉴定-PUMC MDA-MB-453-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:6传代；每周2~3次。 Passage at 1: 2 ~ 1: 6; and passage for 2 to 3 times per week. MDA-MB-453 MDA-MB-453 cell MDA-MB-453 细胞 NICR16 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR16 cell NICR16 细胞 http://www.cellresource.cn/fdetail.aspx?id=16 L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-453-Cell Morphology-PUMC MDA-MB-453-Species Identification-PUMC MDA-MB-453-cell morphology-PUMC MDA-MB-453-物种鉴定-PUMC MDA-MB-453-细胞形态-PUMC MDA-MB-453-细胞形态-PUMC Amelogenin:X；CSF1PO:10,12；D13S317:12；D16S539:9；D18S51:15,20；D19S433:13,14；D21S11:29,31；D2S1338:23,24；D3S1358:15；D5S818:11；D7S820:10；D8S1179:10,12；FGA:18,23；TH01:6；TPOX:10；vWA:17,18 Amelogenin:X；CSF1PO:10,12；D13S317:12；D16S539:9；D18S51:15,20；D19S433:13,14；D21S11:29,31；D2S1338:23,24；D3S1358:15；D5S818:11；D7S820:10；D8S1179:10,12；FGA:18,23；TH01:6；TPOX:10；vWA:17,18 3131C0001000700035 Human Breast Cancer Cells; MDA-MB-453 人乳腺癌细胞；MDA-MB-453 epithelial-like 上皮细胞样 semi-adherent growth 半贴壁生长 This cell is human breast cancer cell line established from pleural fluid. 从胸水中建立的人乳腺癌细胞株。 L15: Leibovitz Medium fetal bovine serum，10% L15: Leibovitz Medium 胎牛血清，10% L15: Leibovitz Medium 胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A MDA-MB-453-DNA-Fingerprinting-SHCRC MDA-MB-453-DNA-指纹-SHCRC MDA-MB-453-cell morphology-SHCRC MDA-MB-453-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 1:2。3天内可长满。 Passage at 1: 2. The cells can be overgrown within 3 days. MDA-MB-453 MDA-MB-453 cell MDA-MB-453 细胞 NICR605 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR605 cell NICR605 细胞 http://www.cellresource.cn/fdetail.aspx?id=605 L15: Leibovitz Medium 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-453-DNA-Fingerprinting-SHCRC MDA-MB-453-DNA-指纹-SHCRC MDA-MB-453-cell morphology-SHCRC MDA-MB-453-细胞形态-SHCRC Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：9；D18S51：17；D19S433：12，13；D21S11：27；D2S1338：17，25；D3S1358：15；D5S818：12；D7S820：8；D8S1179：13；FGA：23；TH01：7；TPOX：8，9；vWA：18； Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：9；D18S51：17；D19S433：12，13；D21S11：27；D2S1338：17，25；D3S1358：15；D5S818：12；D7S820：8；D8S1179：13；FGA：23；TH01：7；TPOX：8，9；vWA：18； 3111C0001CCC000249 Human Breast Cancer Cells; MDA-MB-468 人乳腺癌细胞；MDA-MB-468 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from the pleural effusion of a 51-year-old black woman with metastatic breast cancer by Cailleau R et al in 1977. Although the G6PD allele of donor tissue was heterozygous, the cell line always showed G6PD A phenotype. The G → A mutation in codon 273 of P53 gene resulted in Arg → His substitution. There were 1×106 EGF receptors on each cell. 该细胞是1977年由Cailleau R等从一位患有转移性乳腺癌的51岁黑人女性的胸腔积液中分离得到的。虽然供体组织的G6PD等位基因杂合，但此细胞株始终表现为G6PD A表型。P53基因273位密码子存在G→A突变，从而导致Arg→His替代。每个细胞上存在1×106个EGF受体。 L15: Leibovitz Medium 10%FBS L15: Leibovitz Medium 10%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf C4 C4 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A MDA-MB-468-cell morphology-PUMC MDA-MB-468-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；每周换液2~3次。 Passage at 1: 2 ~ 1: 4; and change fluid for 2~3 times a week. MDA-MB-468 MDA-MB-468 cell MDA-MB-468 细胞 NICR249 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR249 cell NICR249 细胞 http://www.cellresource.cn/fdetail.aspx?id=249 L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 10%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-468-cell morphology-PUMC MDA-MB-468-细胞形态-PUMC 3131C0001000700120 Human Breast Cancer Cells; MDA-MB-468 人乳腺癌细胞；MDA-MB-468 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from the pleural effusion of a 51-year-old black woman with metastatic breast cancer by Cailleau R et al in 1977. Although the G6PD allele of donor tissue was heterozygous, the cell line always showed G6PD A phenotype. The G → A mutation in codon 273 of P53 gene resulted in Arg → His substitution. There were 1×106 EGF receptors on each cell. 该细胞是1977年由Cailleau R等从一位患有转移性乳腺癌的51岁黑人女性的胸腔积液中分离得到的。虽然供体组织的G6PD等位基因杂合，但此细胞株始终表现为G6PD A表型。P53基因273位密码子存在G→A突变，从而导致Arg→His替代。每个细胞上存在1×106个EGF受体。 L15: Leibovitz Medium qualified fetal bovine serum，10% L15: Leibovitz Medium 优质胎牛血清，10% L15: Leibovitz Medium 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P22 P22 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B MDA-MB-468-cell morphology-SHCRC MDA-MB-468-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 10 minutes. Passage at 1: 2. The cells can be overgrowth in 3-4 days 消化10分钟。1:2。3-4天长满。 MDA-MB-468 MDA-MB-468 cell MDA-MB-468 细胞 NICR688 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR688 cell NICR688 细胞 http://www.cellresource.cn/fdetail.aspx?id=688 L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108100839.pdf MDA-MB-468-cell morphology-SHCRC MDA-MB-468-细胞形态-SHCRC Amelogenin：X；CSF1PO：12；D13S317：11，12；D16S539：9；D18S51：10；D19S433：14；D21S11：30，30.2；D2S1338：20；D3S1358：17；D5S818：9，12；D7S820：9，12；D8S1179：12；FGA：20；TH01：8，9；TPOX：8，11；vWA：17； Amelogenin：X；CSF1PO：12；D13S317：11，12；D16S539：9；D18S51：10；D19S433：14；D21S11：30，30.2；D2S1338：20；D3S1358：17；D5S818：9，12；D7S820：9，12；D8S1179：12；FGA：20；TH01：8，9；TPOX：8，11；vWA：17； 3111C0001CCC000085 Human Breast Cancer Cells; SK-BR-3 人乳腺癌细胞；SK-BR-3 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from the pleural effusion of a 43-year-old white female breast cancer patient by Trempe G and Old LJ in 1970. The submicroscopic structural features of the cells include microfilaments and desmosomes, hepatic glycogen granules, macrolysosomes, bundle-forming cytoplasmic fibrils, and overexpression of HER2/ c-erb-2 gene product. 这株细胞是1970年由Trempe G和Old LJ从一位43岁的白人女性乳腺癌患者的胸腔积液中分离得到的。该细胞亚显微结构特征包括微丝和桥粒、肝糖原颗粒、大溶酶体、成束的细胞质纤丝；过表达HER2/c-erb-2基因产物。 RPMI 1640 (w/o Hepes) 10%FBS RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 69~80 69~80 A类 Class A SK-BR-3-Cell Morphology-PUMC SK-BR-3-cell morphology-PUMC SK-BR-3-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2传代；每周换液2~3次。 Passage at 1: 2; and change fluid for 2~3 times a week. NICR85 SK-BR-3 SK-BR-3 cell SK-BR-3 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR85 cell NICR85 细胞 http://www.cellresource.cn/fdetail.aspx?id=85 RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS URI: http://www.cellresource.cn/pdf/20080108101040.pdf SK-BR-3-Cell Morphology-PUMC SK-BR-3-cell morphology-PUMC SK-BR-3-细胞形态-PUMC SK-BR-3-细胞形态-PUMC Amelogenin:X；CSF1PO:12；D13S317:11,12；D16S539:9；D18S51:10,13；D19S433:14；D21S11:30,30.2；D2S1338:20,25；D3S1358:17；D5S818:9,12；D7S820:9,12；D8S1179:12；FGA:20；TH01:8,9；TPOX:8,11；vWA:17 Amelogenin:X；CSF1PO:12；D13S317:11,12；D16S539:9；D18S51:10,13；D19S433:14；D21S11:30,30.2；D2S1338:20,25；D3S1358:17；D5S818:9,12；D7S820:9,12；D8S1179:12；FGA:20；TH01:8,9；TPOX:8,11；vWA:17 3131C0001000700049 Human Breast Cancer Cells; SK-BR-3 人乳腺癌细胞；SK-BR-3 epithelial cell 上皮细胞 adherent growth 贴壁生长 This cell is derived from hydrothorax. There are no particles of virus. Submicroscopic structural features include microfilaments and desmosomes, hepatic glycogen granules, macrolysosomes, and bundle-forming cytoplasmic fibrils. SK-BR-3 cell line overexpresses HER2/c-erb-2 gene product. 这株细胞源自胸水。 没有病毒颗粒。 亚显微结构特征包括微丝和桥粒，肝糖原颗粒，大溶酶体，成束的细胞质纤丝。 SK-BR-3细胞株过表达HER2/c-erb-2基因产物。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf PN5 PN5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A SK-BR-3-cell morphology-SHCRC SK-BR-3-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. 消化3-5分钟。1:2。3天内可长满。 NICR618 SK-BR-3 SK-BR-3 cell SK-BR-3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR618 cell NICR618 细胞 http://www.cellresource.cn/fdetail.aspx?id=618 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf SK-BR-3-cell morphology-SHCRC SK-BR-3-细胞形态-SHCRC STR Identification STR鉴定 3142C0001000000313 Human Breast Cancer Cells; SK-BR-3 [SKBR-3; SKBR3] 人乳腺癌细胞；SK-BR-3 [SKBR-3；SKBR3] epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were deposited by Professor Su Li of Life Science College of Huazhong University of Science and Technology and can be used for basic research. 该细胞由华中科技大学生命科学学院苏莉教授提交保藏，可用于相关的基础研究。 McCoy＇s 5A Media (Modified with Tricine) 10%FBS McCoy＇s 5A Media (Modified with Tricine) 10%FBS http://www.cellresource.cn/pdf/20080108100925.pdf http://www.cellresource.cn/pdf/20080108100925.pdf C24 C24 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 A类 Class A Cell Library of China Center For Type Culture Collection 中国典型培养物保藏中心细胞库 Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 1:3传代,2-3天传一代 Passage at 1: 3, and passage once in 2-3 days. NICR1964 SK-BR-3 SK-BR-3 cell SK-BR-3 细胞 SKBR-3 SKBR-3 cell SKBR-3 细胞 SKBR3 SKBR3 cell SKBR3 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1964 cell NICR1964 细胞 http://www.cellresource.cn/fdetail.aspx?id=1964 McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf McCoy＇s 5A Media (Modified with Tricine) 10%FBS URI: http://www.cellresource.cn/pdf/20080108100925.pdf Amelogenin:X；CSF1PO:10,11；D13S317:10,11；D16S539:9；D18S51:17；D19S433:13.2,14.2；D21S11:31；D2S1338:17,22；D3S1358:15,18；D5S818:12,13；D7S820:11,12；D8S1179:13,14；FGA:22；TH01:8,9；TPOX:10,11；vWA:18,19 Amelogenin:X；CSF1PO:10,11；D13S317:10,11；D16S539:9；D18S51:17；D19S433:13.2,14.2；D21S11:31；D2S1338:17,22；D3S1358:15,18；D5S818:12,13；D7S820:11,12；D8S1179:13,14；FGA:22；TH01:8,9；TPOX:10,11；vWA:18,19 3131C0001001200003 Human Breast Cancer Cells; ZR-75-30 [ZR7530] 人乳腺癌细胞；ZR-75-30 [ZR7530] epithelium-like; polygon 上皮样；多角 adherent growth 贴壁生长 ZR-75-30 is derived from ascites from a 47-year-old black female patient with climacteric invasive ductal carcinoma. The cells were characterized as human, non-HeLa, initiation of malignant breast epithelial carcinoma. ZR-75-30源自一位47岁女性黑人更年期侵入性导管癌患者的腹水。 细胞定性为人，非-HeLa，恶性乳房上皮癌起始。 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Negative 阴性 B类 Class B ZR-75-30-cell morphology-SHCRC ZR-75-30-细胞形态-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 中国科学院上海生命科学研究院细胞资源中心 Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn Digestion for 10 minutes. Passage at 1: 2. The cells can be overgrowth in 5-6 days. 消化10分钟。1:2。5-6天长满。 NICR1584 ZR-75-30 ZR-75-30 cell ZR-75-30 细胞 ZR7530 ZR7530 cell ZR7530 细胞 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 NICR1584 cell NICR1584 细胞 http://www.cellresource.cn/fdetail.aspx?id=1584 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 优质胎牛血清，10% URI: http://www.cellresource.cn/pdf/20080108101040.pdf ZR-75-30-cell morphology-SHCRC ZR-75-30-细胞形态-SHCRC Amelogenin：X；CSF1PO： 10，12；D13S317：11；D16S539：8；D18S51：15；D19S433：15.2；D21S11：32.2；D2S1338：17；D3S1358：18；D5S818：11；D7S820：9，10；D8S1179：14，16；FGA：19；TH01：9.3；TPOX：8；vWA：15； Amelogenin：X；CSF1PO： 10，12；D13S317：11；D16S539：8；D18S51：15；D19S433：15.2；D21S11：32.2；D2S1338：17；D3S1358：18；D5S818：11；D7S820：9，10；D8S1179：14，16；FGA：19；TH01：9.3；TPOX：8；vWA：15； 3111C0001CCC000336 Human Mammary Ductal Carcinoma Cells; BT-549 [BT549] 人乳腺导管癌细胞；BT-549 [BT549] epithelial-like 上皮细胞样 adherent growth 贴壁生长 The cells were established by W.G. Coutinho and E.Y. Lasfargues, and originated from a 72-year-old white female with breast ductal carcinoma. The tissues of origin included nipple and invasive catheter. The cell morphology includes epithelial-like cells and polynuclear giant cells, and can secrete a viscous substance. 该细胞1978年由W.G. Coutinho 和 E.Y. Lasfargues建系，源自一位72岁患有乳腺导管癌的白人女性，来源组织包括乳头及浸润导管。该细胞形态包括上皮样细胞及多核巨细胞，可分泌一种粘性物质。 RPMI 1640 (w/o Hepes) 10%FBS；0.023IU/ml insulin RPMI 1640 (w/o Hepes) 10%FBS；0.023IU/ml insulin http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf P48 P48 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 73～80，XX 73～80，XX A类 Class A BT549-Cell Morphology-PUMC BT549-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:6传代，2～3天换液一次 Passage at 1: 2 ~ 1: 6, 2 to 3 days replacement fluid once BT-549 BT-549 cell BT-549 细胞 BT549 BT549 cell BT549 细胞 NICR1525 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1525 cell NICR1525 细胞 http://www.cellresource.cn/fdetail.aspx?id=1525 RPMI 1640 (w/o Hepes) 10%FBS；0.023IU/ml insulin URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；0.023IU/ml insulin URI: http://www.cellresource.cn/pdf/20080108101040.pdf BT549-Cell Morphology-PUMC BT549-细胞形态-PUMC Amelogenin：X；CSF1PO：12；D13S317：12，14；D16S539：10，14；D18S51：15；D19S433：13，15；D21S11：27，28；D2S1338：17，19；D3S1358：18；D5S818：9；D7S820：10；D8S1179：8，10；FGA：24；TH01：9.3；TPOX：9，12；vWA：16，17； Amelogenin：X；CSF1PO：12；D13S317：12，14；D16S539：10，14；D18S51：15；D19S433：13，15；D21S11：27，28；D2S1338：17，19；D3S1358：18；D5S818：9；D7S820：10；D8S1179：8，10；FGA：24；TH01：9.3；TPOX：9，12；vWA：16，17； 3111C0001CCC000220 Human Mammary Ductal Carcinoma Cells; HCC38 人乳腺导管癌细胞；HCC38 epithelioid 上皮样 adherent growth 贴壁生长 The cells were isolated from the breast ductal carcinoma of a 50-year-old white female in 1992. The patient had a history of leiomyosarcoma and her mother died of breast cancer; the cells contained oncogene her2/neu-, p53+; deections for Epithelial Cell Specific Markers EGP2 (Epithelial Glycoprotein 2) and CK19 were positive, while those for ER and PR were negative. 该细胞于1992年从一位50岁的白人女性乳腺导管癌组织中分离建立，该患者曾有平滑肌肉瘤的病史，其母亲死于乳腺癌；该细胞癌基因her2/neu-，p53+；上皮细胞特异性标志物EGP2（上皮糖蛋白2）和CK19阳性，ER和PR阴性。 RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋1mM sodium pyruvate RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋1mM sodium pyruvate http://www.cellresource.cn/pdf/20080108101040.pdf http://www.cellresource.cn/pdf/20080108101040.pdf C5 C5 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A HCC38-Species Identification-PUMC HCC38-cell morphology-PUMC HCC38-物种鉴定-PUMC HCC38-细胞形态-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2~1:4传代；3~5天换液1次。 Passage at 1: 2 ~ 1: 4; and change fluid once in 3~5 days. HCC38 HCC38 cell HCC38 细胞 NICR220 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR220 cell NICR220 细胞 http://www.cellresource.cn/fdetail.aspx?id=220 RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108101040.pdf RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋1mM sodium pyruvate URI: http://www.cellresource.cn/pdf/20080108101040.pdf HCC38-Species Identification-PUMC HCC38-cell morphology-PUMC HCC38-物种鉴定-PUMC HCC38-细胞形态-PUMC Amelogenin：X；CSF1PO：10，12；D13S317：8；D16S539：13；D18S51：10，16；D19S433：13，15；D21S11：26，28；D2S1338：17，18；D3S1358：15，17；D5S818：11，12；D7S820：10，11；D8S1179：14；FGA：21，22；TH01：6，9；TPOX：8，11；vWA：14，15； Amelogenin：X；CSF1PO：10，12；D13S317：8；D16S539：13；D18S51：10，16；D19S433：13，15；D21S11：26，28；D2S1338：17，18；D3S1358：15，17；D5S818：11，12；D7S820：10，11；D8S1179：14；FGA：21，22；TH01：6，9；TPOX：8，11；vWA：14，15； 3111C0001CCC000380 Human Mammary Ductal Carcinoma Cells; MDA-MB-134Ⅵ 人乳腺导管癌细胞；MDA-MB-134Ⅵ epithelial-like 上皮细胞样 loosely adherent growth 松散贴壁生长 The cells were established by R. Cailleau in 1973, derived from pleural effusion of a 74-year-old female breast ductal carcinoma patient; the cells grow slowly and loosely, falling off to the culture medium during growth, and do not converge and overexpress FGF receptors. 该细胞1973年由R. Cailleau建系，源自74岁乳腺导管癌女性患者的胸腔积液，细胞生长缓慢，松散贴壁，生长过程中会脱落到培养基，不会汇合，过表达FGF受体。 L15: Leibovitz Medium 20%FBS L15: Leibovitz Medium 20%FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf P37 P37 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:4传代，每周换液2～3次 Passage at 1: 2 ~ 1: 4, and change fluid for 2 to 3 times a week MDA-MB-134Ⅵ MDA-MB-134Ⅵ cell MDA-MB-134Ⅵ 细胞 NICR1569 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1569 cell NICR1569 细胞 http://www.cellresource.cn/fdetail.aspx?id=1569 L15: Leibovitz Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf L15: Leibovitz Medium 20%FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf Amelogenin：X，Y；CSF1PO：7，8；D13S317：11，12；D16S539：11，13；D18S51：15；D19S433：12.2，15；D21S11：29，30；D2S1338：OL，22；D3S1358：16，18；D5S818：11；D7S820：11，12；D8S1179：14，17；FGA：23，26；TH01：7；TPOX：6，8；vWA：15，16； Amelogenin：X，Y；CSF1PO：7，8；D13S317：11，12；D16S539：11，13；D18S51：15；D19S433：12.2，15；D21S11：29，30；D2S1338：OL，22；D3S1358：16，18；D5S818：11；D7S820：11，12；D8S1179：14，17；FGA：23，26；TH01：7；TPOX：6，8；vWA：15，16； 3111C0001CCC000349 Human Mammary Ductal Carcinoma Cells; MDA-MB-175Ⅶ 人乳腺导管癌细胞；MDA-MB-175Ⅶ epithelial-like 上皮细胞样 loosely adherent 松散贴壁 The cells are derived from the pleural effusion of a 54-year-old white woman with breast ductal carcinoma. 该细胞源自一位54岁患有乳腺导管癌白人女性的胸腔积液。 L15: Leibovitz Medium 10% FBS L15: Leibovitz Medium 10% FBS http://www.cellresource.cn/pdf/20080108100839.pdf http://www.cellresource.cn/pdf/20080108100839.pdf C2 C2 Base Medium+8%DMSO+20%FBS 基础培养基+8%DMSO+20%FBS Culture Method（-） 培养法（-） 82～89 82～89 A类 Class A Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences 中国医学科学院基础医学研究所基础医学细胞中心 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 1:2～1:6传代，每周换液2～3次 Passage at 1: 2 ~ 1: 6, and change fluid for 2 to 3 times a week MDA-MB-175Ⅶ MDA-MB-175Ⅶ cell MDA-MB-175Ⅶ 细胞 NICR1538 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 NICR1538 cell NICR1538 细胞 http://www.cellresource.cn/fdetail.aspx?id=1538 L15: Leibovitz Medium 10% FBS URI: http://www.cellresource.cn/pdf/20080108100839.pdf