identifier: traitmech:000700
label: cytoplasmic bacterial predation
definition: A predatory bacterial phenotype in which a bacterium enters the cytoplasm
  of a bacterial prey cell and grows and reproduces there by consuming and degrading
  the prey cytoplasm.
definition_source: DOI:10.1073/pnas.83.7.2138
trait_category: ECOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- traitmech:000054
evidence:
- reference: DOI:10.1073/pnas.83.7.2138
  snippet: Daptobacter penetrates both membranes of the prey cell walls and reproduces
    in their degrading cytoplasm.
  notes: 'Guerrero et al. 1986, PMID:11542073, PMC323246, Figure 2E caption continued
    on p. 2141. Full five-page primary paper read from the Europe PMC PDF, including
    Methods, Results and Discussion, Table 2 and the note added in proof; actual Figure
    2E/F micrographs and continued caption visually inspected. Enrichment-culture
    thin sections support prey-cytoplasmic invasion, degradation and division; these
    are not live-cell time courses or nutrient-tracing experiments. Figure 3 is a
    schematic, not additional experimental imaging. The scientific abstract was independently
    retrieved with EXT_ID:11542073 AND SRC:MED and title/DOI checked. MED and Crossref
    exposed no linked correction/retraction; publisher notice access returned HTTP
    403, so that status is unverified. Source: https://europepmc.org/articles/PMC323246?pdf=render.'
- reference: https://lpsn.dsmz.de/genus/daptobacter
  snippet: invade their host cells and replicate in the cytoplasm, consuming their
    prey from the inside out
  notes: LPSN genus Daptobacter, Notes, directly retrieved 2026-10-11. LPSN quotes
    this description from Madigan et al. 2019; that book was not independently read.
    Supports established terminology, not independent experimental replication of
    Guerrero et al. LPSN marks the genus name not validly published and preferred
    rather than correct. Its permanent record link is https://doi.org/10.83108/rn.684.
    The nomenclatural record is not evidence for a sequence-defined lineage or an
    NCBITaxon mapping.
discussions:
- discussion_id: cytoplasmic-predation-boundaries-and-mappings
  prompt: Keep prey-cytoplasmic feeding distinct from generic intracellular residence.
  kind: CURATION_TODO
  status: OPEN
  rationale: Predatory bacterium traitmech:000054 supplies the broader trophic phenotype.
    Periplasmic bacterial predation traitmech:000699 has a different replication compartment;
    epibiotic bacterial predation traitmech:000697 keeps the predator cell body external.
    Generic endobiotic or intracellular predation, endoparasitism and endosymbiosis
    are not exact synonyms. Guerrero et al. discuss predator/parasite and primary-consumer
    terminology on pp. 2141-2142; the operational feeding/location definition here
    does not settle that broader ecological vocabulary. Entry alone or intracellular
    growth without destructive prey consumption is insufficient. No organism-level
    disjointness, fixed prey range or obligate prey dependence is asserted. Exact
    synonyms and authority-backed xrefs remain unassigned pending scope reconciliation.
  posed_by: codex
  posed_date: '2026-10-11'
- discussion_id: cytoplasmic-predation-taxon-and-mechanism
  prompt: Resolve the historical isolate and its mechanism before grounding examples.
  kind: KNOWLEDGE_GAP
  status: OPEN
  rationale: The primary study describes Daptobacter in lake-derived enrichment cultures
    and reports axenic growth; it does not make cytoplasmic predation an obligate
    lifestyle. No exact NCBITaxon identity for the observed material has been established
    in this curation, so canonical_examples is intentionally omitted. LPSN nomenclature
    does not supply that missing mapping. The second evidence item is terminology
    support, not another experiment. Protein-resolved mechanism, modern replication
    and lineage breadth remain open; do not infer causal machinery from a taxon name,
    sequence prediction or the different Bdellovibrio model. No causal graph or protein
    examples are proposed.
  posed_by: codex
  posed_date: '2026-10-11'
curation_history:
- timestamp: '2026-10-11T15:30:45Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Added cytoplasmic bacterial predation beneath predatory bacterium with
    primary microscopy and explicitly secondary LPSN terminology evidence, both carrying
    checked snippets. Ignored/hidden main/worktree and pending-PR checks support 000700
    and v576 block 1065300-1065399. Existing records unchanged; taxon identity, mappings
    and mechanism remain open. Timestamp records the observed UTC curation decision.
  llm_assisted: true