identifier: traitmech:000726 label: fungal nematode egg parasitism definition: An animal-pathogenic phenotype in which a fungus infects nematode eggs by penetrating their eggshells and colonizing their interiors. definition_source: DOI:10.3389/fmicb.2021.617710 trait_category: ECOLOGY term_kind: CLASS mapping_status: PROPOSED parent_traits: - METPO:1004002 evidence: - reference: DOI:10.3389/fmicb.2021.617710 snippet: The egg-pathogenic fungus Purpureocillium lilacinum parasitizes on nematode eggs notes: Xu et al. 2021, PMID:33897634, PMCID:PMC8058359, scientific Abstract; direct JATS match and native VERIFIED. Own JZD02 experiment, Methods and actual Figure 1 A-C support infection of Meloidogyne incognita eggs. Supplementary Table 1 defines infection by hyphal penetration through an individual egg, not mere surface contact. Its 30-hour printed mean disagrees with the replicate counts; no numerical infection rates are adopted. The 6-hour control and 42-hour infected transcriptomes differ in time and include mixed infection stages. Expression associations are not causal protein evidence. - reference: DOI:10.3389/fmicb.2016.01084 snippet: Infected eggs by P. lilacinum 36-1. notes: Xie et al. 2016, PMID:27486440, PMCID:PMC4949223, Figure 5 caption, panel B; actual panels A-C and Methods/Results inspected. P. lilacinum denotes Purpureocillium lilacinum. Direct JATS match; native NOT_IN_ABSTRACT is retained. This is its own 36-1 infection experiment, not a repetition of Xu's citation. Eggs were supplied as the only nourishment for the reported 24-hour comparison, but microscopy does not quantify nutrient assimilation. RNA-seq replicates were pooled; gene homology and expression do not demonstrate a required effector. - reference: DOI:10.3389/fmicb.2021.617710 snippet: Infection defined by Purpureocillium lilacinum mycelium penetrating through the whole individual Meloidogyne incognita egg notes: Xu et al. 2021, Supplementary Table 1 footnote, Table_1.DOCX from the PMC8058359 Europe PMC supplementaryFiles archive. Direct DOCX text match and complete rendered page inspected; native NOT_IN_ABSTRACT is retained. This operational scoring criterion supports individual-egg penetration, not another independent experiment or a direct nutrient-flux measurement. The two Xu excerpts total 23 words. The printed 30-hour mean discrepancy remains unresolved; no infection rate is imported. discussions: - discussion_id: fungal-nematode-egg-parasitism-scope-and-hierarchy prompt: Refine the egg-infection hierarchy without conflating infection, feeding and hatching endpoints. kind: CURATION_TODO status: OPEN rationale: 'The fungal bearer penetrates eggshells and colonizes egg interiors. Surface attachment, egg-hatching inhibition, killing, trapping or a gene annotation alone is insufficient. Animal pathogen METPO:1004002 is active below pathogenic to host METPO:1004000 and phenotype METPO:1000059. Its pinned organism-like wording is clarified as a phenotype in current upstream METPO; the local parent is not rewritten. Nematode egg infection does not entail mammalian pathogenicity. Xie describes this fungus as nematophagous, but the operational infection endpoint does not quantify feeding: fungal nematophagy traitmech:000719 requires consumption and also includes dead prey. It is related, not an asserted parent here. Local parasitism traitmech:000043 inherits persistent symbiosis, which is not required. No organism-level disjointness, obligacy, exclusive host stage or universal host range is asserted. Bare egg parasitism does not specify the fungal actor and is not an exact synonym.' posed_by: codex posed_date: '2026-10-14' - discussion_id: fungal-nematode-egg-parasitism-metpo-assessment prompt: Review the proposed class without reviving the obsolete parasitism identifier. kind: CURATION_TODO status: OPEN rationale: No exact active counterpart was identified in the inspected pinned/current releases, editor and template owners. Historical METPO:1000227 obsolete parasitism is broader, deprecated, under ObsoleteClass with OMO:0001000 reason and no replacement or consider annotation in those releases. METPO issue 521 disputes mechanically assigned reasons; the code neither invalidates the biology nor authorizes reuse. The historical ID is not revived or placed in xrefs. Fried-egg colony is unrelated. The ontology pin remains unchanged; v602 proposes this narrower infection phenotype with PROPOSED status and no equivalent external xref. posed_by: codex posed_date: '2026-10-14' - discussion_id: fungal-nematode-egg-parasitism-evidence-limits prompt: Resolve source inconsistencies and strain provenance before adding rates, examples or causal mechanisms. kind: KNOWLEDGE_GAP status: OPEN rationale: Xu Supplementary Table 1 reports a 30-hour mean of 38% for replicates 38%, 33% and 33%, whose arithmetic mean is about 34.7%. This discrepancy is preserved, not silently corrected. Its main Methods sample-level wording is less precise than the supplement's individual-egg penetration criterion. No rates or infection threshold are curated. Xie's conserved appressorium genes also occur in fungi lacking that structure; neither gene inventory nor differential expression establishes this trait or a protein-resolved mechanism. No causal graph, protein example or canonical taxon is asserted without further authority and strain-provenance checks. Microscopy and source-designated infection are not tracer measurements of nutrient flux. No field efficacy or safety claim follows. Source hashes, inspected figures/supplement, actual snippet verdicts and bounded notice checks are retained in the verification sidecar; no comprehensive correction/retraction clearance is claimed. Both counted primary experiments concern Purpureocillium lilacinum (JZD02 and 36-1). The fungal actor class is not evidence that this phenotype was experimentally demonstrated across fungal species; additional species require their own evidence. posed_by: codex posed_date: '2026-10-14' curation_history: - timestamp: '2026-10-14T11:46:22Z' curator: codex action: MINTED_TRAITMECH_ID changes: Added fungal nematode egg parasitism with two primary DOI sources and directly matched nine-word and six-word excerpts. Preserved native VERIFIED/NOT_IN_ABSTRACT outcomes and the supplement's inconsistent mean; no rates or sequence-derived mechanism imported. Reserved 000726, v602 and 1067900-1067999 after ignored/hidden current-copy, retained-history and open/draft PR checks, including full-content proposal ranges. Wheel sizes and broad editor envelopes are not conflicting reservations. Animal-pathogen parent scope checked against pinned/current ontology. Other records unchanged; timestamp records observed acceptance. llm_assisted: true - timestamp: '2026-10-14T12:50:22Z' curator: codex action: CLARIFY_EVIDENCE changes: 'Addressed issue #1931 after the approving PR review: added a directly matched 14-word supplementary penetration criterion and explicitly limited the two primary experiments to one fungal species. Xu quotations total 23 words; the extra excerpt is not a third independent experiment. Retained native NOT_IN_ABSTRACT, original minting history, definition, parent, source discrepancy and PROPOSED status.' llm_assisted: true