identifier: METPO:1000470 label: NaCl range mid1 definition: A NaCl range phenotype in which the growth-supporting NaCl range spans approximately 1–3% (w/v), characteristic of slight-halophilic or halotolerant organisms. definition_source: DOI:10.1093/femsre/fuy009 trait_category: ENVIRONMENT term_kind: CLASS mapping_status: REVIEWED parent_traits: - METPO:1000334 synonyms: - synonym_text: Halotolerant synonym_type: EXACT_SYNONYM source: metpo.owl - synonym_text: Slight halophile synonym_type: EXACT_SYNONYM source: metpo.owl - synonym_text: NaR_1_to_3 synonym_type: RELATED_SYNONYM source: metpo.owl evidence: - reference: DOI:10.1093/femsre/fuy009 snippet: slight halophile notes: Osmoadaptation review supports the 1–3% NaCl growth range as the slight-halophile/halotolerant category. causal_graphs: - graph_id: nacl_range_mid1_slight_halophile title: NaCl-range-mid1 slight-halophile range description: DOI-backed graph linking modest osmoadaptive tolerance to a 1–3% (w/v) NaCl growth range. nodes: - node_id: nacl_range_mid1_trait label: NaCl range mid1 node_type: TRAIT grounding: METPO:1000470 description: NaCl growth-supporting range approximately 1–3% w/v. - node_id: nacl_range label: NaCl range node_type: TRAIT grounding: METPO:1000334 description: Span of NaCl concentrations supporting growth. - node_id: modest_osmoadaptive_tolerance label: modest osmoadaptive tolerance node_type: BIOLOGICAL_PROCESS description: Osmoadaptive capacity supporting growth across seawater-range NaCl. - node_id: slight_halophile label: slight halophile node_type: TRAIT description: Organisms that grow best in media with 1-3% NaCl. - node_id: moderate_halophile label: moderate halophile node_type: TRAIT description: Organisms growing optimally in 3-15% NaCl (0.5-2.5 M salt). - node_id: optimal_growth_0_5_to_2_5_M_salt label: optimal growth between 0.5 and 2.5 M salt node_type: QUALITY description: Salt optimum defining moderate halophiles. - node_id: potassium_accumulation label: K+ accumulation node_type: BIOLOGICAL_PROCESS description: Intracellular accumulation of potassium ions for osmotic balancing. - node_id: osmotic_balance label: osmotic balance / salt adaptation node_type: BIOLOGICAL_PROCESS description: Maintenance of cellular osmotic homeostasis under salt stress. - node_id: compatible_solute_strategy label: compatible solute strategy node_type: BIOLOGICAL_PROCESS description: Osmoadaptation via ion exclusion and production of compatible solutes such as glycine betaine. - node_id: halotolerant_and_moderate_halophiles label: halotolerant and moderate halophiles node_type: TRAIT description: Organisms tolerating or requiring moderate salinity. edges: - subject: modest_osmoadaptive_tolerance predicate: produces object: nacl_range_mid1_trait description: Modest osmoadaptive tolerance yields a 1–3% NaCl growth range. evidence: - reference: DOI:10.1093/femsre/fuy009 snippet: slight halophile notes: Supports the 1–3% range as the slight-halophile outcome. predicate_id: METPO:2000202 - subject: nacl_range_mid1_trait predicate: is a object: nacl_range description: NaCl range mid1 is a quantitative bin of the NaCl-range phenotype. evidence: - reference: DOI:10.1093/femsre/fuy009 snippet: salinity range notes: Supports the 1–3% range as a value within the NaCl-range distribution. predicate_id: rdfs:subClassOf - subject: nacl_range_mid1_trait predicate: corresponds to object: slight_halophile description: The 1-3% w/v NaCl range corresponds to the slight-halophile phenotype. evidence: - reference: DOI:10.1007/978-3-540-74231-9 notes: slight halophiles grow best in media with 1 to 3% NaCl - subject: slight_halophile predicate: distinct from object: moderate_halophile description: Slight halophiles are distinct from moderate halophiles, which grow optimally at higher NaCl. evidence: - reference: DOI:10.1007/978-3-540-74231-9 notes: moderate halophiles grow optimally in media with 3 to 15% NaCl - subject: moderate_halophile predicate: defined as object: optimal_growth_0_5_to_2_5_M_salt description: Moderate halophiles are defined as organisms growing optimally between 0.5 and 2.5 M salt. evidence: - reference: DOI:10.1128/MMBR.62.2.504-544.1998 notes: moderate halophiles as organisms growing optimally between 0.5 and 2.5 M salt - subject: potassium_accumulation predicate: contributes to object: osmotic_balance description: K+ accumulation contributes to osmotic balance and salt adaptation. evidence: - reference: DOI:10.1128/AEM.00145-24 notes: simultaneously accumulating compatible solutes and K+; general osmoadaptation edge predicate_id: RO:0002326 - subject: compatible_solute_strategy predicate: characterizes object: halotolerant_and_moderate_halophiles description: The compatible-solute strategy characterizes halotolerant and moderate halophiles. evidence: - reference: DOI:10.1093/FEMSRE/FUY026 notes: halotolerant and moderate halophiles rely on excluding ions and producing compatible solutes such as glycine betaine curation_history: - timestamp: '2026-05-05T01:35:46.836372+00:00' curator: seed_from_metpo action: SEEDED_FROM_METPO changes: imported from data/raw/metpo.owl (CLASS) llm_assisted: false - timestamp: '2026-05-12T10:00:00-07:00' curator: claude action: CURATED_CAUSAL_GRAPH changes: Added DOI-backed definition and causal graph linking modest osmoadaptive tolerance to the slight-halophile NaCl-range bin. llm_assisted: true - timestamp: '2026-05-20T03:35:30Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 2 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (METPO:2000202×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-06-23T00:00:00Z' curator: claude action: ENRICH_CAUSAL_GRAPH changes: Added 5 evidence-backed generic edges (7 new nodes) from the deep-research report. llm_assisted: true - timestamp: '2026-06-24T17:21:10Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 1 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002326×1). llm_assisted: true