identifier: METPO:1000471 label: NaCl range mid2 definition: A NaCl range phenotype in which the growth-supporting NaCl range spans approximately 3–8% (w/v), characteristic of moderate-halophile organisms. definition_source: DOI:10.1093/femsre/fuy009 trait_category: ENVIRONMENT term_kind: CLASS mapping_status: REVIEWED parent_traits: - METPO:1000334 synonyms: - synonym_text: Halotolerant synonym_type: EXACT_SYNONYM source: metpo.owl - synonym_text: Moderate halophile synonym_type: EXACT_SYNONYM source: metpo.owl - synonym_text: NaR_3_to_8 synonym_type: RELATED_SYNONYM source: metpo.owl evidence: - reference: DOI:10.1093/femsre/fuy009 snippet: moderate halophile notes: Osmoadaptation review supports the 3–8% NaCl growth range as the moderate-halophile category. causal_graphs: - graph_id: nacl_range_mid2_moderate_halophile title: NaCl-range-mid2 moderate-halophile range description: DOI-backed graph linking robust osmoadaptive tolerance to a 3–8% (w/v) NaCl growth range. nodes: - node_id: nacl_range_mid2_trait label: NaCl range mid2 node_type: TRAIT grounding: METPO:1000471 description: NaCl growth-supporting range approximately 3–8% w/v. - node_id: nacl_range label: NaCl range node_type: TRAIT grounding: METPO:1000334 description: Span of NaCl concentrations supporting growth. - node_id: robust_osmoadaptive_tolerance label: robust osmoadaptive tolerance node_type: BIOLOGICAL_PROCESS description: Strong compatible-solute and ion-balance capacity sustaining growth at saline-lake / salt-marsh NaCl levels. - node_id: ectABC_operon label: ectABC operon node_type: GENE_OR_PROTEIN description: Gene cluster (ectA, ectB, ectC) encoding the ectoine biosynthesis pathway. - node_id: ectoine label: ectoine node_type: CHEMICAL description: Compatible solute / osmoprotectant accumulated under salt stress. grounding: CHEBI:27592 - node_id: na_k_ion_uptake label: Na+ and K+ ion uptake node_type: BIOLOGICAL_PROCESS description: Rapid uptake of sodium and potassium ions following NaCl upshift. - node_id: osmotic_pressure_balance label: osmotic pressure balance node_type: BIOLOGICAL_PROCESS description: Balancing of intracellular osmotic pressure after a NaCl upshift. - node_id: glutamate_glutamine_pools label: intracellular glutamate/glutamine pools node_type: CHEMICAL description: Elevated glutamate and glutamine amino-acid pools providing early osmotic buffering. - node_id: betAB_pathway label: BetA/BetB pathway node_type: GENE_OR_PROTEIN description: Choline dehydrogenase (BetA) and betaine aldehyde dehydrogenase (BetB) converting choline to glycine betaine. - node_id: glycine_betaine label: glycine betaine node_type: CHEMICAL description: Compatible solute synthesized from choline; osmoprotectant. grounding: CHEBI:17750 edges: - subject: robust_osmoadaptive_tolerance predicate: produces object: nacl_range_mid2_trait description: Robust osmoadaptive tolerance yields a 3–8% NaCl growth range. evidence: - reference: DOI:10.1093/femsre/fuy009 snippet: moderate halophile notes: Supports the 3–8% range as the moderate-halophile outcome. predicate_id: METPO:2000202 - subject: nacl_range_mid2_trait predicate: is a object: nacl_range description: NaCl range mid2 is a quantitative bin of the NaCl-range phenotype. evidence: - reference: DOI:10.1093/femsre/fuy009 snippet: salinity range notes: Supports the 3–8% range as a value within the NaCl-range distribution. predicate_id: rdfs:subClassOf - subject: ectABC_operon predicate: enables biosynthesis of object: ectoine description: The ectABC operon encodes the conserved ectoine biosynthesis pathway. evidence: - reference: DOI:10.3389/fmicb.2023.1192059 notes: ectoine biosynthesis relies on ectABC; repeatedly supported across taxa. - subject: ectoine predicate: positively contributes to object: nacl_range_mid2_trait description: Ectoine accumulation supports sustained growth/tolerance at 6-8% NaCl. evidence: - reference: DOI:10.1186/s12934-024-02358-5 notes: Within tolerable range (1-8% NaCl shock) ectoine rapidly becomes the dominant osmoprotectant. - subject: na_k_ion_uptake predicate: rapidly balances object: osmotic_pressure_balance description: Uptake of Na+ and K+ ions rapidly balances osmotic pressure after NaCl upshift. evidence: - reference: DOI:10.1186/s12934-024-02358-5 notes: Cells urgently balanced surging osmotic pressure by uptaking sodium and potassium ions. - subject: osmotic_pressure_balance predicate: contributes to object: robust_osmoadaptive_tolerance description: Balancing osmotic pressure underpins robust osmoadaptive tolerance. evidence: - reference: DOI:10.1186/s12934-024-02358-5 notes: Rapid ion-flux osmotic balancing is part of the osmoadaptive response sustaining growth. predicate_id: RO:0002326 - subject: glutamate_glutamine_pools predicate: provides early osmotic buffering for object: robust_osmoadaptive_tolerance description: Increased glutamate/glutamine pools provide early osmotic buffering during NaCl shock. evidence: - reference: DOI:10.1186/s12934-024-02358-5 notes: Augmenting intracellular amino acid pools, particularly glutamate and glutamine, buffers early osmotic stress. - subject: betAB_pathway predicate: enables biosynthesis of object: glycine_betaine description: BetA and BetB convert choline to glycine betaine, a compatible solute. evidence: - reference: DOI:10.3389/fmicb.2023.1192059 notes: Choline to glycine betaine via BetA and BetB; conserved across moderate halophiles. - subject: glycine_betaine predicate: positively contributes to object: robust_osmoadaptive_tolerance description: Glycine betaine acts as a compatible solute supporting osmoadaptive tolerance. evidence: - reference: DOI:10.3389/fmicb.2023.1192059 notes: Glycine betaine biosynthesis/uptake supports compatible-solute-based osmoadaptation. curation_history: - timestamp: '2026-05-05T01:35:46.836940+00:00' curator: seed_from_metpo action: SEEDED_FROM_METPO changes: imported from data/raw/metpo.owl (CLASS) llm_assisted: false - timestamp: '2026-05-12T10:00:00-07:00' curator: claude action: CURATED_CAUSAL_GRAPH changes: Added DOI-backed definition and causal graph linking robust osmoadaptive tolerance to the moderate-halophile NaCl-range bin. llm_assisted: true - timestamp: '2026-05-20T03:35:30Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 2 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (METPO:2000202×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-06-23T00:00:00Z' curator: claude action: ENRICH_CAUSAL_GRAPH changes: Added 7 evidence-backed generic edges (7 new nodes) from the deep-research report. llm_assisted: true - timestamp: '2026-06-24T17:21:10Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 1 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002326×1). llm_assisted: true - timestamp: '2026-06-24T17:23:31Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 2 causal-node grounding field(s) via mappings/node_grounding.tsv (CHEBI:27592×1, CHEBI:17750×1). llm_assisted: true