identifier: METPO:1000485 label: temperature delta mid1 definition: A temperature delta phenotype with a growth-supporting temperature breadth of approximately 10–20 °C, characteristic of organisms with moderate thermal-tolerance breadth. definition_source: DOI:10.1146/annurev-micro-091313-103612 trait_category: ENVIRONMENT term_kind: CLASS mapping_status: REVIEWED parent_traits: - METPO:1000303 synonyms: - synonym_text: Td_10_20 synonym_type: RELATED_SYNONYM source: metpo.owl evidence: - reference: DOI:10.1146/annurev-micro-091313-103612 snippet: more unsaturated fatty acids notes: Membrane-adaptation review supports moderate homoviscous remodeling capacity as common among generalist mesophiles. causal_graphs: - graph_id: temperature_delta_mid1_moderate_breadth title: Temperature-delta-mid1 moderate-breadth thermal adaptation description: DOI-backed graph linking moderate thermal-adaptation flexibility to a 10–20 °C temperature growth breadth. nodes: - node_id: temperature_delta_mid1_trait label: temperature delta mid1 node_type: TRAIT grounding: METPO:1000485 description: Temperature growth-supporting breadth approximately 10–20 °C. - node_id: temperature_delta label: temperature delta node_type: TRAIT grounding: METPO:1000303 description: Breadth of the growth-supporting ambient-temperature range. - node_id: moderate_thermal_adaptation label: moderate thermal-adaptation flexibility node_type: BIOLOGICAL_PROCESS description: Mid-range capacity to remodel membrane lipids and enzymes across temperature shifts of ~10–20 °C. - node_id: temperature_decrease label: decrease in ambient temperature node_type: ENVIRONMENTAL_FACTOR description: A drop in ambient growth temperature, the proximal cue for cold membrane adaptation. - node_id: membrane_unsaturated_fatty_acids label: membrane unsaturated fatty acids node_type: CHEMICAL description: Cis-unsaturated acyl chains in membrane phospholipids whose abundance sets membrane fluidity. - node_id: membrane_fluidity label: membrane fluidity node_type: QUALITY description: Physical fluid state of the lipid bilayer that must be maintained across temperature shifts. grounding: METPO:1007505 - node_id: ufa_biosynthesis label: cis-unsaturated fatty acid biosynthesis node_type: BIOLOGICAL_PROCESS description: Synthesis of cis-unsaturated fatty acids that remodel membrane lipids during cooling. - node_id: membrane_physical_state_change label: membrane physical-state change node_type: BIOLOGICAL_PROCESS description: Cold-induced change in the liquid-crystalline state of the membrane acting as a sensor event. - node_id: two_component_cold_signaling label: two-component cold signaling node_type: BIOLOGICAL_PROCESS description: Two-component signal transduction triggering the cold-adaptation transcriptional response. - node_id: thermal_niche_breadth label: thermal niche / temperature breadth node_type: QUALITY description: Thermal tolerance niche operationally bounded by growth-supporting Tmin and Tmax. - node_id: tmin_tmax label: Tmin and Tmax growth boundaries node_type: QUALITY description: Minimum and maximum growth-supporting temperatures that flank the thermal niche. edges: - subject: moderate_thermal_adaptation predicate: produces object: temperature_delta_mid1_trait description: Moderate thermal-adaptation flexibility yields a 10–20 °C temperature-delta breadth. evidence: - reference: DOI:10.1146/annurev-micro-091313-103612 snippet: more unsaturated fatty acids notes: Supports moderate remodeling flexibility as the basis of an intermediate breadth. predicate_id: METPO:2000202 - subject: temperature_delta_mid1_trait predicate: is a object: temperature_delta description: Temperature delta mid1 is a quantitative bin of the temperature-delta phenotype. evidence: - reference: DOI:10.1016/s0300-9629(97)00003-0 snippet: adapted to environments of high temperature notes: Supports the 10–20 °C breadth as a value within the temperature-delta distribution. predicate_id: rdfs:subClassOf - subject: temperature_decrease predicate: increases object: ufa_biosynthesis description: Colder temperature shifts fatty-acid synthesis toward cis-unsaturated products to preserve membrane function. evidence: - reference: DOI:10.1007/s42770-023-01057-4 notes: 'Ramon 2023 review: increased unsaturation (especially cis-MUFA) on temperature decrease; broad review support.' predicate_id: RO:0002213 - subject: ufa_biosynthesis predicate: increases object: membrane_unsaturated_fatty_acids description: Cis-unsaturated fatty acid biosynthesis raises the unsaturated acyl-chain content of membrane lipids. evidence: - reference: DOI:10.1007/s42770-023-01057-4 notes: 'Ramon 2023: microbes increase cis-unsaturation to remodel membranes, a general cold-adaptation output.' predicate_id: RO:0002213 - subject: membrane_unsaturated_fatty_acids predicate: increases object: membrane_fluidity description: More unsaturated acyl chains counteract cold-induced rigidification and restore membrane fluidity. evidence: - reference: DOI:10.1128/spectrum.03925-23 notes: 'Sidarta 2024: desaturation fluidizes membranes; central homeoviscous-adaptation edge, generalizable.' predicate_id: RO:0002213 - subject: membrane_physical_state_change predicate: activates object: two_component_cold_signaling description: Cold-induced changes in the membrane liquid-crystalline state act as the upstream sensor activating two-component cold signaling. evidence: - reference: DOI:10.1007/s42770-023-01057-4 notes: 'Ramon 2023: cold sensing via changes in liquid-crystalline state of membranes that activate two-component signalling.' predicate_id: RO:0002213 - subject: membrane_fluidity predicate: enables object: moderate_thermal_adaptation description: Maintained membrane fluidity across temperature shifts underlies the moderate thermal-adaptation flexibility that supports the breadth. evidence: - reference: DOI:10.1128/spectrum.03925-23 notes: 'Sidarta 2024: homeoviscous adaptation restores fluidity, the membrane-target of thermal adaptation.' predicate_id: RO:0002327 - subject: tmin_tmax predicate: defines object: thermal_niche_breadth description: Growth-supporting minimum and maximum temperatures flank the thermal function and mark the thermal niche/breadth. evidence: - reference: DOI:10.3390/pr8010121 notes: 'Noll 2020: Tmin and Tmax for growth flank the function and mark the thermal tolerance/niche of an organism.' predicate_id: METPO:2007500 - subject: thermal_niche_breadth predicate: is a object: temperature_delta description: The Tmin/Tmax-bounded thermal niche is the breadth quantity captured by the temperature-delta phenotype. evidence: - reference: DOI:10.3390/pr8010121 notes: 'Noll 2020: thermal breadth treated as a cardinal-temperature bounded niche property.' predicate_id: rdfs:subClassOf curation_history: - timestamp: '2026-05-05T01:35:46.844372+00:00' curator: seed_from_metpo action: SEEDED_FROM_METPO changes: imported from data/raw/metpo.owl (CLASS) llm_assisted: false - timestamp: '2026-05-13T11:00:00-07:00' curator: claude action: CURATED_CAUSAL_GRAPH changes: Added DOI-backed definition and causal graph linking moderate thermal-adaptation flexibility to the temperature-delta-mid1 bin. llm_assisted: true - timestamp: '2026-05-20T03:35:30Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 2 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (METPO:2000202×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-06-23T00:00:00Z' curator: claude action: ENRICH_CAUSAL_GRAPH changes: Added 7 evidence-backed generic edges (8 new nodes) from the deep-research report. llm_assisted: true - timestamp: '2026-06-24T17:21:11Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 7 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002213×4, RO:0002327×1, METPO:2007500×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-06-24T17:21:17Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 1 causal-node grounding field(s) via mappings/node_grounding.tsv (METPO:1007505×1). llm_assisted: true