identifier: METPO:1000454 label: temperature range high definition: A temperature range phenotype in which the growth-supporting ambient temperature range extends above approximately 40 °C, characteristic of thermophilic physiology. definition_source: DOI:10.1016/s0300-9629(97)00003-0 trait_category: ENVIRONMENT term_kind: CLASS mapping_status: REVIEWED parent_traits: - METPO:1000306 synonyms: - synonym_text: Thermophile synonym_type: EXACT_SYNONYM source: metpo.owl - synonym_text: TR_>40 synonym_type: RELATED_SYNONYM source: metpo.owl evidence: - reference: DOI:10.1016/s0300-9629(97)00003-0 snippet: adapted to environments of high temperature notes: Thermophile-adaptation review supports growth ranges extending above 40 °C as the thermophile category. - reference: DOI:10.1128/MMBR.65.1.1-43.2001 snippet: resistant to irreversible inactivation at high temperatures notes: Thermostable-protein review supports thermostability as the mechanism extending growth into thermophilic temperatures. causal_graphs: - graph_id: temperature_range_high_thermophile title: Temperature-range-high thermophile range description: DOI-backed graph linking thermophile thermostability to a temperature growth range extending above 40 °C. nodes: - node_id: temperature_range_high_trait label: temperature range high node_type: TRAIT grounding: METPO:1000454 description: Temperature growth-supporting range extending above ~40 °C. - node_id: temperature_range label: temperature range node_type: TRAIT grounding: METPO:1000306 description: Span of ambient temperatures supporting growth. - node_id: thermophile_thermostability label: thermophile thermostability node_type: BIOLOGICAL_PROCESS description: Heat-stable membrane lipids and proteins that retain function at elevated temperatures. - node_id: reverse_gyrase label: reverse gyrase (TopR/rgy) node_type: GENE_OR_PROTEIN description: Topoisomerase that introduces positive supercoils into DNA in thermophiles. - node_id: positive_dna_supercoiling label: positive DNA supercoiling node_type: QUALITY description: Positively supercoiled DNA topology generated by reverse gyrase. - node_id: dna_melting label: DNA melting node_type: BIOLOGICAL_PROCESS description: Thermal denaturation/strand separation of DNA at elevated temperature. - node_id: dnak_dnaj label: DnaK-DnaJ chaperone node_type: GENE_OR_PROTEIN description: Hsp70 chaperone system assisting ATP-dependent protein folding. grounding: UniProtKB:A0A0C1PL72 - node_id: protein_folding label: protein folding node_type: BIOLOGICAL_PROCESS description: Folding of nascent and unfolded polypeptides into native conformation. grounding: GO:0006457 - node_id: groel_groes_htpg label: GroEL-GroES / HtpG chaperone node_type: GENE_OR_PROTEIN description: Chaperonin and Hsp90 systems assisting protein folding under heat stress. - node_id: proteostasis_high_temp label: proteostasis at high temperature node_type: BIOLOGICAL_PROCESS description: Maintenance of protein homeostasis (folding/refolding/degradation) at elevated temperature. edges: - subject: thermophile_thermostability predicate: enables object: temperature_range_high_trait description: Thermophile thermostability enables growth at > 40 °C. evidence: - reference: DOI:10.1128/MMBR.65.1.1-43.2001 snippet: resistant to irreversible inactivation at high temperatures notes: Supports thermostability as the range mechanism for thermophile physiology. predicate_id: RO:0002327 - subject: temperature_range_high_trait predicate: is a object: temperature_range description: Temperature range high is a quantitative bin of the temperature-range phenotype. evidence: - reference: DOI:10.1016/s0300-9629(97)00003-0 snippet: adapted to environments of high temperature notes: Supports the >40 °C range as a value within the temperature-range distribution. predicate_id: rdfs:subClassOf - subject: reverse_gyrase predicate: introduces object: positive_dna_supercoiling description: Reverse gyrase introduces positive supercoils into DNA. evidence: - reference: DOI:10.1264/jsme2.me23087 notes: reverse gyrase, a unique topoisomerase that introduces positive supercoils into DNA; broadly applicable to thermophiles. - subject: positive_dna_supercoiling predicate: limits object: dna_melting description: Positive DNA supercoiling limits thermal melting of DNA. evidence: - reference: DOI:10.1264/jsme2.me23087 notes: maintain the genome integrity of thermophiles by limiting DNA melting; general for reverse-gyrase-bearing thermophiles. predicate_id: RO:0002212 - subject: reverse_gyrase predicate: enables object: temperature_range_high_trait description: Reverse gyrase activity supports growth at high temperature via genome stabilization. evidence: - reference: DOI:10.1128/mbio.02174-23 notes: Reverse gyrase reported as essential for growth at very high temperature, maintaining DNA stability. predicate_id: RO:0002327 - subject: dnak_dnaj predicate: assists object: protein_folding description: DnaK-DnaJ assists ATP-dependent folding of nascent and unfolded proteins. evidence: - reference: DOI:10.1007/s12275-023-00031-x notes: DnaK-DnaJ assist ATP-dependent folding of nascent and unfolded peptides; bacterial high-temperature response. - subject: groel_groes_htpg predicate: assists object: protein_folding description: GroEL-GroES and HtpG assist protein folding under heat stress. evidence: - reference: DOI:10.1007/s12275-023-00031-x notes: GroEL-GroES, HtpG assist protein folding under heat stress; broadly relevant heat response. - subject: protein_folding predicate: maintains object: proteostasis_high_temp description: Chaperone-assisted folding maintains proteostasis at high temperature. evidence: - reference: DOI:10.1128/mbio.02174-23 notes: refolding/degradation systems counter protein denaturation, aggregation and loss of function during heat shock. - subject: proteostasis_high_temp predicate: enables object: temperature_range_high_trait description: Proteostasis at high temperature supports growth above 40 C. evidence: - reference: DOI:10.1128/mbio.02174-23 notes: proteostasis machinery supports thermophile survival/growth at high temperature. predicate_id: RO:0002327 curation_history: - timestamp: '2026-05-05T01:35:46.825852+00:00' curator: seed_from_metpo action: SEEDED_FROM_METPO changes: imported from data/raw/metpo.owl (CLASS) llm_assisted: false - timestamp: '2026-05-13T11:00:00-07:00' curator: claude action: CURATED_CAUSAL_GRAPH changes: Added DOI-backed definition and causal graph linking thermophile thermostability to the temperature-range-high bin. llm_assisted: true - timestamp: '2026-05-20T03:35:30Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 2 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002327×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-06-23T00:00:00Z' curator: claude action: ENRICH_CAUSAL_GRAPH changes: Added 7 evidence-backed generic edges (7 new nodes) from the deep-research report. llm_assisted: true - timestamp: '2026-06-24T17:21:11Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 3 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002327×2, RO:0002212×1). llm_assisted: true - timestamp: '2026-06-24T17:23:32Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 1 causal-node grounding field(s) via mappings/node_grounding.tsv (GO:0006457×1). llm_assisted: true - timestamp: '2026-06-26T07:15:18Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 1 causal-node grounding field(s) via mappings/node_grounding.tsv (UniProtKB:A0A0C1PL72×1). llm_assisted: true