identifier: METPO:1000449 label: temperature range low definition: A temperature range phenotype in which the growth-supporting ambient temperature range spans approximately 10–22 °C, characteristic of psychrophilic or psychrotolerant physiology. definition_source: DOI:10.1038/sj.embor.7400662 trait_category: ENVIRONMENT term_kind: CLASS mapping_status: REVIEWED parent_traits: - METPO:1000306 synonyms: - synonym_text: Psychrophile synonym_type: EXACT_SYNONYM source: metpo.owl - synonym_text: Psychrotolerant synonym_type: EXACT_SYNONYM source: metpo.owl - synonym_text: TR_10_to_22 synonym_type: RELATED_SYNONYM source: metpo.owl evidence: - reference: DOI:10.1038/sj.embor.7400662 snippet: decreased membrane fluidity notes: Psychrophile review supports cool-temperature membrane and enzyme adaptation as the basis of growth in the 10–22 °C range. causal_graphs: - graph_id: temperature_range_low_psychrotolerant title: Temperature-range-low psychrotolerant range description: DOI-backed graph linking psychrotolerant adaptation to a temperature growth range of approximately 10–22 °C. nodes: - node_id: temperature_range_low_trait label: temperature range low node_type: TRAIT grounding: METPO:1000449 description: Temperature growth-supporting range approximately 10–22 °C. - node_id: temperature_range label: temperature range node_type: TRAIT grounding: METPO:1000306 description: Span of ambient temperatures supporting growth. - node_id: psychrotolerant_adaptation label: psychrotolerant adaptation node_type: BIOLOGICAL_PROCESS description: Mild cold-adaptation of membrane and enzyme function sustaining growth at cool temperatures. - node_id: low_temperature label: low temperature node_type: ENVIRONMENTAL_FACTOR description: Cool ambient temperature in the approximately 10-22 C range. grounding: PATO:0001306 - node_id: membrane_fluidity label: membrane fluidity node_type: QUALITY description: Physical fluidity state of the cytoplasmic membrane lipid bilayer. grounding: METPO:1007505 - node_id: fatty_acid_desaturase_activity label: fatty acid desaturase activity node_type: MOLECULAR_FUNCTION description: Enzymatic introduction of double bonds into fatty acyl chains (fatty acid desaturases, EC 1.14.19.-). - node_id: unsaturated_fatty_acid_proportion label: unsaturated fatty acid proportion node_type: QUALITY description: Relative abundance of unsaturated fatty acids in membrane lipids. - node_id: cold_shock_proteins label: cold-shock proteins node_type: GENE_OR_PROTEIN description: CspA/CspC family cold-shock proteins acting as RNA chaperones. - node_id: rna_chaperone_activity label: RNA chaperone activity node_type: MOLECULAR_FUNCTION description: Resolution of inhibitory RNA secondary structures supporting translation at low temperature. - node_id: compatible_solutes label: compatible solutes node_type: CHEMICAL description: Glycine betaine, trehalose and glycerol accumulated as cryoprotectants. grounding: CHEBI:25728 - node_id: cryoprotection label: cryoprotection node_type: BIOLOGICAL_PROCESS description: Stabilization of proteins and membranes and protection against cold/freezing damage. - node_id: molecular_chaperones label: GroEL/DnaK molecular chaperones node_type: GENE_OR_PROTEIN description: GroEL and DnaK chaperones assisting protein folding under temperature stress. - node_id: protein_misfolding label: protein misfolding node_type: BIOLOGICAL_PROCESS description: Cold-denaturation and misfolding of proteins at low temperature. edges: - subject: psychrotolerant_adaptation predicate: enables object: temperature_range_low_trait description: Psychrotolerant adaptation enables growth across 10–22 °C. evidence: - reference: DOI:10.1038/sj.embor.7400662 snippet: Cold-shock proteins have also been described notes: Supports cold-shock and acclimation responses as the cool-range mechanism. predicate_id: RO:0002327 - subject: temperature_range_low_trait predicate: is a object: temperature_range description: Temperature range low is a quantitative bin of the temperature-range phenotype. evidence: - reference: DOI:10.1038/sj.embor.7400662 snippet: growing well at temperatures around the freezing point of water notes: Supports the 10–22 °C range as a value within the temperature-range distribution. predicate_id: rdfs:subClassOf - subject: low_temperature predicate: decreases object: membrane_fluidity description: Low temperature reduces membrane fluidity, triggering compensatory lipid remodeling. evidence: - reference: DOI:10.1007/s42770-023-01057-4 notes: Cold reduces membrane fluidity; changes in membrane physical state mediate cold sensing. predicate_id: RO:0002212 - subject: fatty_acid_desaturase_activity predicate: increases object: membrane_fluidity description: Fatty acid desaturase activity raises unsaturation to restore membrane fluidity at low temperature. evidence: - reference: DOI:10.37256/amtt.5220244537 notes: Genes for desaturation are upregulated; desaturases enhance membrane fluidity via unsaturated/PUFA synthesis. predicate_id: RO:0002213 - subject: low_temperature predicate: increases object: unsaturated_fatty_acid_proportion description: Growth at low temperature increases the proportion of unsaturated fatty acids in membrane lipids. evidence: - reference: DOI:10.1128/aem.01928-22 notes: Proportion of unsaturated fatty acids was higher in strains cultured at low temperatures while long-chain SFAs decreased. predicate_id: RO:0002213 - subject: cold_shock_proteins predicate: enables object: rna_chaperone_activity description: Cold-shock proteins act as RNA chaperones supporting translation at low temperature. evidence: - reference: DOI:10.3389/fmicb.2023.1197797 notes: Cold shock proteins (Csps) can function as RNA chaperones, supporting translation in the cold. predicate_id: RO:0002327 - subject: compatible_solutes predicate: provides object: cryoprotection description: Compatible solutes provide cryoprotection by stabilizing membranes and proteins and lowering freezing point. evidence: - reference: DOI:10.3389/fmicb.2023.1197797 notes: Compatible osmolytes prevent cell shrinkage, lower freezing point, scavenge radicals, and stabilize membranes/proteins. - subject: molecular_chaperones predicate: prevents object: protein_misfolding description: GroEL/DnaK chaperones prevent cold-denaturation and protein misfolding at low temperature. evidence: - reference: DOI:10.37256/amtt.5220244537 notes: Chaperones are upregulated at low temperatures to prevent cold-denaturation and misfolding. predicate_id: RO:0002212 curation_history: - timestamp: '2026-05-05T01:35:46.823166+00:00' curator: seed_from_metpo action: SEEDED_FROM_METPO changes: imported from data/raw/metpo.owl (CLASS) llm_assisted: false - timestamp: '2026-05-13T11:00:00-07:00' curator: claude action: CURATED_CAUSAL_GRAPH changes: Added DOI-backed definition and causal graph linking psychrotolerant adaptation to the temperature-range-low bin. llm_assisted: true - timestamp: '2026-05-20T03:35:30Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 2 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002327×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-06-23T00:00:00Z' curator: claude action: ENRICH_CAUSAL_GRAPH changes: Added 6 evidence-backed generic edges (10 new nodes) from the deep-research report. llm_assisted: true - timestamp: '2026-06-24T17:21:11Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 5 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002212×2, RO:0002213×2, RO:0002327×1). llm_assisted: true - timestamp: '2026-06-24T17:21:18Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 3 causal-node grounding field(s) via mappings/node_grounding.tsv (PATO:0001306×1, METPO:1007505×1, CHEBI:25728×1). llm_assisted: true