identifier: METPO:1000452 label: temperature range mid3 definition: A temperature range phenotype in which the growth-supporting ambient temperature range spans approximately 30–34 °C, characteristic of mesophilic physiology. definition_source: DOI:10.1146/annurev-micro-091313-103612 trait_category: ENVIRONMENT term_kind: CLASS mapping_status: REVIEWED parent_traits: - METPO:1000306 synonyms: - synonym_text: Mesophilie synonym_type: EXACT_SYNONYM source: metpo.owl - synonym_text: TR_30_to_34 synonym_type: RELATED_SYNONYM source: metpo.owl evidence: - reference: DOI:10.1146/annurev-micro-091313-103612 snippet: more unsaturated fatty acids notes: Membrane-adaptation review supports the 30–34 °C range as the upper-mesophile growth range. causal_graphs: - graph_id: temperature_range_mid3_upper_mesophile title: Temperature-range-mid3 upper-mesophile range description: DOI-backed graph linking upper-mesophile adaptation to a temperature growth range of approximately 30–34 °C. nodes: - node_id: temperature_range_mid3_trait label: temperature range mid3 node_type: TRAIT grounding: METPO:1000452 description: Temperature growth-supporting range approximately 30–34 °C. - node_id: temperature_range label: temperature range node_type: TRAIT grounding: METPO:1000306 description: Span of ambient temperatures supporting growth. - node_id: upper_mesophile_adaptation label: upper-mesophile adaptation node_type: BIOLOGICAL_PROCESS description: Mesophile membrane and enzyme tuning for ambient temperatures near common animal host body temperatures. - node_id: membrane_order label: membrane order / fluidity state node_type: QUALITY description: Physical order/fluidity state of the membrane bilayer; the proximate signal for thermosensing. - node_id: desk_kinase_state label: DesK kinase-dominant state node_type: STATE description: Membrane thickness sensor DesK in its autophosphorylating kinase-dominant conformation. - node_id: desk label: DesK node_type: GENE_OR_PROTEIN description: Membrane-bound histidine kinase that senses membrane order/thickness. grounding: UniProtKB:A0A067Z5N7 - node_id: desr label: DesR node_type: GENE_OR_PROTEIN description: Response regulator activated by DesK to control des transcription. grounding: UniProtKB:A0A067Z2X2 - node_id: phospho_desr label: phosphorylated DesR node_type: STATE description: Phosphorylated DesR that tetramerizes and binds the des promoter. - node_id: des_gene label: des (acyl-lipid desaturase) node_type: GENE_OR_PROTEIN description: Fatty-acid desaturase that introduces double bonds to fluidize the membrane. - node_id: membrane_fatty_acyl_chains label: membrane fatty acyl chains node_type: CHEMICAL description: Fatty acyl chains of membrane phospholipids whose unsaturation modulates fluidity. - node_id: membrane_rigidification label: membrane rigidification and increased thickness node_type: BIOLOGICAL_PROCESS description: Cooling-induced membrane rigidification and bilayer thickening, the general homeoviscous sensing signal. - node_id: temperature_decrease label: temperature decrease node_type: ENVIRONMENTAL_FACTOR description: Drop in ambient temperature within/near the mesophilic range. - node_id: homeoviscous_adaptation label: homeoviscous adaptation node_type: BIOLOGICAL_PROCESS description: Adjustment of membrane lipid composition to maintain constant fluidity across temperatures. - node_id: liquid_crystalline_membrane label: liquid-crystalline membrane state node_type: STATE description: Functional liquid-crystalline phase of the membrane required for growth. edges: - subject: upper_mesophile_adaptation predicate: enables object: temperature_range_mid3_trait description: Upper-mesophile adaptation enables growth across 30–34 °C. evidence: - reference: DOI:10.1146/annurev-micro-091313-103612 snippet: more unsaturated fatty acids notes: Supports upper-mesophile homeoviscous adaptation as the range mechanism. predicate_id: RO:0002327 - subject: temperature_range_mid3_trait predicate: is a object: temperature_range description: Temperature range mid3 is a quantitative bin of the temperature-range phenotype. evidence: - reference: DOI:10.1016/s0300-9629(97)00003-0 snippet: adapted to environments of high temperature notes: Supports the 30–34 °C range as a value within the temperature-range distribution. predicate_id: rdfs:subClassOf - subject: membrane_order predicate: drives object: desk_kinase_state description: Increased membrane order (reduced fluidity) drives DesK to a kinase-dominant state; membrane physical state is the proximate signal. evidence: - reference: DOI:10.1128/mmbr.00069-23 notes: increased membrane order (reduced fluidity) drives DesK to a kinase-dominant state - subject: desk predicate: phosphorylates object: desr description: Core two-component signaling step linking membrane physical state to transcriptional response. evidence: - reference: DOI:10.1128/spectrum.03925-23 notes: DesK autophosphorylates (His188) and phosphorylates DesR - subject: phospho_desr predicate: activates transcription of object: des_gene description: DesR-P tetramerizes, binds the des promoter and induces lipid desaturation to restore membrane fluidity. evidence: - reference: DOI:10.1128/spectrum.03925-23 notes: P-DesR tetramerizes, binds Pdes, and activates des expression - subject: des_gene predicate: introduces double bonds into object: membrane_fatty_acyl_chains description: Des-mediated unsaturation fluidizes the membrane and reduces bilayer thickness. evidence: - reference: DOI:10.1128/spectrum.03925-23 notes: Des introduces double bonds into fatty acyl chains, fluidizing the membrane and reducing thickness - subject: temperature_decrease predicate: causes object: membrane_rigidification description: General physical trigger underlying homeoviscous adaptation across microbes. evidence: - reference: DOI:10.1007/s42770-023-01057-4 notes: membrane rigidification and increased thickness are proposed as sensing signals that trigger adaptive responses predicate_id: biolink:causes - subject: homeoviscous_adaptation predicate: maintains object: liquid_crystalline_membrane description: 'Broad mechanism relevant to upper-mesophile growth: cells preserve membrane function across temperature fluctuations.' evidence: - reference: DOI:10.1007/s42770-023-01057-4 notes: maintain the liquid-crystalline phase at low temperature curation_history: - timestamp: '2026-05-05T01:35:46.824789+00:00' curator: seed_from_metpo action: SEEDED_FROM_METPO changes: imported from data/raw/metpo.owl (CLASS) llm_assisted: false - timestamp: '2026-05-13T11:00:00-07:00' curator: claude action: CURATED_CAUSAL_GRAPH changes: Added DOI-backed definition and causal graph linking upper-mesophile adaptation to the temperature-range-mid3 bin. llm_assisted: true - timestamp: '2026-05-20T03:35:30Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 2 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002327×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-06-23T00:00:00Z' curator: claude action: ENRICH_CAUSAL_GRAPH changes: Added 6 evidence-backed generic edges (11 new nodes) from the deep-research report. llm_assisted: true - timestamp: '2026-06-24T17:21:11Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 1 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (biolink:causes×1). llm_assisted: true - timestamp: '2026-06-26T07:15:18Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 2 causal-node grounding field(s) via mappings/node_grounding.tsv (UniProtKB:A0A067Z5N7×1, UniProtKB:A0A067Z2X2×1). llm_assisted: true