identifier: traitmech:000470
label: DS-30 system
definition: A phage defense system in which an organism possesses the four-gene DefensePredictor-discovered
  system 30 locus cataloged as working transcriptional unit ABC3 and whose plasmid
  expression in E. coli MG1655 reduced bacteriophage plaquing.
definition_source: DOI:10.1126/science.adv7924
trait_category: GENOMICS
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- traitmech:000232
synonyms:
- synonym_text: DS-30
  synonym_type: EXACT_SYNONYM
  source: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
- synonym_text: ABC3
  synonym_type: RELATED_SYNONYM
  source: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
- synonym_text: Lamassu-Fam__Lmu_DS-30A
  synonym_type: RELATED_SYNONYM
  source: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
- synonym_text: Lamassu-Fam__LmuA_DS-30B
  synonym_type: RELATED_SYNONYM
  source: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
- synonym_text: Lamassu-Fam__LmuB_DS-30D
  synonym_type: RELATED_SYNONYM
  source: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
- synonym_text: Lamassu-Fam__LmuC_DS-30C
  synonym_type: RELATED_SYNONYM
  source: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
evidence:
- reference: DOI:10.1126/science.adv7924
  snippet: To test for anti-phage defense, we placed each TU with its predicted native
    promoter region on a low-copy number plasmid in E. coli MG1655 and challenged
    these strains with a panel of 24 diverse E. coli phages (Fig. 3; fig. S2). In
    total, 42 (45% of 94) of the cloned TUs produced smaller plaque sizes or reduced
    the efficiency of plating (EOP) at least ten-fold relative to an empty vector
    control strain
  notes: DeWeirdt et al. experimentally validated 42 predicted transcriptional units
    as phage-defense systems in E. coli.
- reference: DOI:10.1126/science.adv7924
  snippet: We refer to these validated TUs as DefensePredictor discovered systems
    (DSs), with genes in multi-gene TUs denoted by an alphabetical suffix, e.g., DS-8A
    is the first gene of DS-8.
  notes: DeWeirdt et al. name validated transcriptional units as DefensePredictor
    discovered systems.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
  snippet: "ABC3\tNZ_QOYR01000032.1\tGCF_003333645.1\t+\tTrue\tFalse\tTrue\tTrue\t\
    DefensePredictor hits\t1906\t4621\thypothetical protein, hypothetical protein,\
    \ hypothetical protein, DUF2326 domain-containing protein\tWP_001553315.1, WP_000534626.1,\
    \ WP_000628292.1, WP_087889773.1\t8.95063706554687\t6.906754778648663\tTrue\t\
    True\tPredicted novel defense gene\tDS-30"
  notes: The final Science supplementary Table S6 maps working_id ABC3 to DS_name
    DS-30, marks the cloned transcriptional unit as defensive, and records four product
    accessions in NZ_QOYR01000032.1 positions 1906-4621.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
  snippet: "SECphi27\t5.1\tpLAND\t24-05-31_EV_CRDO_BRNT_ABC3.png\t100000000\tABC3\t\
    24-05-31\t24-05-31_EV_CRDO_BRNT_ABC3.png\t5\t1\tY\t100000\t3\t\tTrue\t\tM9\t30"
  notes: The final Science supplementary Table S7 Systems sheet reports an ABC3 assay
    row with a SECphi27 phage readout, a smaller-plaque-size call, and a -log(EOP)
    value of 3.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
  snippet: "ABC3\tDS-30\tTrue"
  notes: The final Science supplementary Table S8 maps ABC3 to replicated display
    name DS-30.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
  snippet: "ABC3\t2.0\t182.0\tWP_000534626.1\tABC-3C C-term\tPF20275.3\tCTD10 ; C-terminal\
    \ domain 10 of the ABC-three component (ABC-3C) systems\thhpred_7102862.hhr\t\
    26.0\t176.0\t1.0\t2024-07-30 00:00:00"
  notes: The final Science supplementary Table S8 reports a high-probability CTD10
    HHpred hit for ABC3 protein 2, WP_000534626.1.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
  snippet: "ABC3\t3.0\t77.0\tWP_000628292.1\tABC-3C Mid\tPF20293.3\tMC6 ; ABC-three\
    \ component (ABC-3C) system Middle Component 6\thhpred_3406401.hhr\t1.0\t75.0\t\
    1.0\t2024-07-30 00:00:00"
  notes: The final Science supplementary Table S8 reports a high-probability MC6 HHpred
    hit for ABC3 protein 3, WP_000628292.1.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
  snippet: "ABC3\t4.0\t565.0\tWP_087889773.1\tABC ATPase\t5XEI_A\tChromosome partition\
    \ protein Smc; Condensin, Smc, head domain, ABC-ATPase,\thhpred_6417130.hhr\t\
    1.0\t540.0\t1.0\t2024-07-30 00:00:00"
  notes: The final Science supplementary Table S8 reports a high-probability ABC ATPase
    HHpred hit for ABC3 protein 4, WP_087889773.1.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
  snippet: "ABC3\t1.0\t86.0\tWP_001553315.1\tFtsL\t8HHF_L\tCell division protein FtsL;\
    \ Bacterial cell division, divisome, FtsB, FtsL, FtsQ, FtsBLQ, membrane protein\
    \ complex, heterotrimer, MEMBRANE PROTEIN\thhpred_2515914.hhr\t2.0\t53.0\t0.942\t\
    2024-07-30 00:00:00"
  notes: The final Science supplementary Table S8 reports an FtsL HHpred hit for ABC3
    protein 1, WP_001553315.1.
- reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
  snippet: '| Lamassu-Fam__Lmu_DS-30A                          |                                                  |
    Lamassu-Fam            | Custom                  | 50     |'
  notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__Lmu_DS-30A as
    a custom Lamassu-Fam profile.
- reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
  snippet: '| Lamassu-Fam__LmuA_DS-30B                         |                                                  |
    Lamassu-Fam            | Custom                  | 60     |'
  notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuA_DS-30B as
    a custom Lamassu-Fam profile.
- reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
  snippet: '| Lamassu-Fam__LmuB_DS-30D                         |                                                  |
    Lamassu-Fam            | Custom                  | 150    |'
  notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuB_DS-30D as
    a custom Lamassu-Fam profile.
- reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
  snippet: '| Lamassu-Fam__LmuC_DS-30C                         |                                                  |
    Lamassu-Fam            | Custom                  | 20     |'
  notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuC_DS-30C as
    a custom Lamassu-Fam profile.
causal_graphs:
- graph_id: ds_30_locus_reduces_phage_plaquing
  title: DS-30 locus reduces bacteriophage plaquing
  description: Conservative system-level sketch linking the four-gene DS-30 locus
    to reduced bacteriophage plaquing without resolving DS-30 component functions
    or molecular output.
  scope_status: NONMECHANISTIC
  scope_notes: The graph captures DS-30 as the validated ABC3 transcriptional unit
    with four product accessions, ABC-3C, SMC-family, and FtsL HHpred-domain rows,
    and four Lamassu-Fam custom HMM-profile rows. It does not assert native host breadth,
    exact profile-to-protein correspondence, DS-30/Lamassu-Fam component activity,
    Lamassu subtype chemistry, LmuC requirement, complete phage breadth, or DS-30-specific
    DefenseFinder rule-level detection criteria.
  nodes:
  - node_id: ds_30_locus
    label: DS-30 locus
    node_type: GENETIC_ELEMENT
    description: A four-gene DefensePredictor-discovered system 30 locus cataloged
      as ABC3 and represented in the pinned DefenseFinder HMM inventory by four Lamassu-Fam
      DS-30 custom profiles.
  - node_id: reduced_phage_plaquing
    label: reduced bacteriophage plaquing
    node_type: BIOLOGICAL_PROCESS
    description: Reduced bacteriophage plaquing in cells carrying cloned ABC3.
  - node_id: ds_30_system_trait
    label: DS-30 system
    node_type: TRAIT
    grounding: traitmech:000470
    description: Possession of a genome-encoded DS-30 Lamassu-family phage-defense
      system.
  - node_id: lamassu_system
    label: Lamassu system
    node_type: TRAIT
    grounding: traitmech:000232
    description: Possession of a genome-encoded Lamassu phage-defense system.
  edges:
  - subject: ds_30_locus
    predicate: contributes to
    predicate_id: RO:0002326
    object: reduced_phage_plaquing
    description: The DS-30/ABC3 locus contributes to reduced bacteriophage plaquing
      in heterologous E. coli MG1655 plasmid-expression assays.
    evidence:
    - reference: DOI:10.1126/science.adv7924
      snippet: To test for anti-phage defense, we placed each TU with its predicted
        native promoter region on a low-copy number plasmid in E. coli MG1655 and
        challenged these strains with a panel of 24 diverse E. coli phages (Fig. 3;
        fig. S2). In total, 42 (45% of 94) of the cloned TUs produced smaller plaque
        sizes or reduced the efficiency of plating (EOP) at least ten-fold relative
        to an empty vector control strain
      notes: DeWeirdt et al. describe the plasmid-based phage challenge used to validate
        predicted transcriptional units.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
      snippet: "ABC3\tNZ_QOYR01000032.1\tGCF_003333645.1\t+\tTrue\tFalse\tTrue\tTrue\t\
        DefensePredictor hits\t1906\t4621\thypothetical protein, hypothetical protein,\
        \ hypothetical protein, DUF2326 domain-containing protein\tWP_001553315.1,\
        \ WP_000534626.1, WP_000628292.1, WP_087889773.1\t8.95063706554687\t6.906754778648663\t\
        True\tTrue\tPredicted novel defense gene\tDS-30"
      notes: The final Science supplementary Table S6 maps working_id ABC3 to DS_name
        DS-30, marks the cloned transcriptional unit as defensive, and records four
        product accessions in NZ_QOYR01000032.1 positions 1906-4621.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
      snippet: "SECphi27\t5.1\tpLAND\t24-05-31_EV_CRDO_BRNT_ABC3.png\t100000000\t\
        ABC3\t24-05-31\t24-05-31_EV_CRDO_BRNT_ABC3.png\t5\t1\tY\t100000\t3\t\tTrue\t\
        \tM9\t30"
      notes: The final Science supplementary Table S7 Systems sheet reports an ABC3
        assay row with a SECphi27 phage readout, a smaller-plaque-size call, and a
        -log(EOP) value of 3.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
      snippet: "ABC3\tDS-30\tTrue"
      notes: The final Science supplementary Table S8 maps ABC3 to replicated display
        name DS-30.
  - subject: reduced_phage_plaquing
    predicate: confers
    predicate_id: METPO:2007700
    object: ds_30_system_trait
    description: DS-30-mediated phage plaquing reduction realizes the DS-30 system
      trait.
    evidence:
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
      snippet: "ABC3\tNZ_QOYR01000032.1\tGCF_003333645.1\t+\tTrue\tFalse\tTrue\tTrue\t\
        DefensePredictor hits\t1906\t4621\thypothetical protein, hypothetical protein,\
        \ hypothetical protein, DUF2326 domain-containing protein\tWP_001553315.1,\
        \ WP_000534626.1, WP_000628292.1, WP_087889773.1\t8.95063706554687\t6.906754778648663\t\
        True\tTrue\tPredicted novel defense gene\tDS-30"
      notes: The final Science supplementary Table S6 maps working_id ABC3 to DS_name
        DS-30, marks the cloned transcriptional unit as defensive, and records four
        product accessions in NZ_QOYR01000032.1 positions 1906-4621.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
      snippet: "SECphi27\t5.1\tpLAND\t24-05-31_EV_CRDO_BRNT_ABC3.png\t100000000\t\
        ABC3\t24-05-31\t24-05-31_EV_CRDO_BRNT_ABC3.png\t5\t1\tY\t100000\t3\t\tTrue\t\
        \tM9\t30"
      notes: The final Science supplementary Table S7 Systems sheet reports an ABC3
        assay row with a SECphi27 phage readout, a smaller-plaque-size call, and a
        -log(EOP) value of 3.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
      snippet: "ABC3\tDS-30\tTrue"
      notes: The final Science supplementary Table S8 maps ABC3 to replicated display
        name DS-30.
    - reference: DOI:10.1126/science.adv7924
      snippet: We refer to these validated TUs as DefensePredictor discovered systems
        (DSs), with genes in multi-gene TUs denoted by an alphabetical suffix, e.g.,
        DS-8A is the first gene of DS-8.
      notes: The DS nomenclature is used for DefensePredictor discovered systems.
  - subject: ds_30_system_trait
    predicate: is a
    predicate_id: rdfs:subClassOf
    object: lamassu_system
    description: DS-30 system possession is represented in DefenseFinder by DS-30
      custom profiles under Lamassu-Fam.
    evidence:
    - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
      snippet: '| Lamassu-Fam__Lmu_DS-30A                          |                                                  |
        Lamassu-Fam            | Custom                  | 50     |'
      notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__Lmu_DS-30A
        as a custom Lamassu-Fam profile.
    - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
      snippet: '| Lamassu-Fam__LmuA_DS-30B                         |                                                  |
        Lamassu-Fam            | Custom                  | 60     |'
      notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuA_DS-30B
        as a custom Lamassu-Fam profile.
    - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
      snippet: '| Lamassu-Fam__LmuB_DS-30D                         |                                                  |
        Lamassu-Fam            | Custom                  | 150    |'
      notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuB_DS-30D
        as a custom Lamassu-Fam profile.
    - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
      snippet: '| Lamassu-Fam__LmuC_DS-30C                         |                                                  |
        Lamassu-Fam            | Custom                  | 20     |'
      notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuC_DS-30C
        as a custom Lamassu-Fam profile.
discussions:
- discussion_id: ds-30-lamassu-family-model-gap
  prompt: Resolve DS-30 native host breadth, exact profile-to-protein correspondence,
    DS-30/Lamassu-Fam component activities, Lamassu subtype and effector chemistry,
    LmuC requirement, complete phage breadth, and DS-30-specific DefenseFinder rule-level
    criteria before minting narrower DS-30 mechanism children.
  kind: KNOWLEDGE_GAP
  status: OPEN
  rationale: DeWeirdt et al. support DS-30 as the defensive ABC3 transcriptional unit
    and final Science Tables S6/S7/S8 map it to four product accessions, a SECphi27
    phage readout, four HHpred-domain rows, and display name DS-30. The pinned DefenseFinder
    HMM inventory records four Lamassu-Fam DS-30 custom profile rows. The pinned DefenseFinder
    rules table only lists generic Lamassu-Fam effector-subtype rows, and the first-pass
    record does not resolve native host breadth, complete phage breadth, direct profile-to-protein
    correspondence, LmuC requirement, molecular output, subtype-specific Lamassu chemistry,
    or endogenous DS-30 activity.
  evidence:
  - reference: DOI:10.1126/science.adv7924
    snippet: We refer to these validated TUs as DefensePredictor discovered systems
      (DSs), with genes in multi-gene TUs denoted by an alphabetical suffix, e.g.,
      DS-8A is the first gene of DS-8.
    notes: DeWeirdt et al. name validated TUs as DSs.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
    snippet: "ABC3\tNZ_QOYR01000032.1\tGCF_003333645.1\t+\tTrue\tFalse\tTrue\tTrue\t\
      DefensePredictor hits\t1906\t4621\thypothetical protein, hypothetical protein,\
      \ hypothetical protein, DUF2326 domain-containing protein\tWP_001553315.1, WP_000534626.1,\
      \ WP_000628292.1, WP_087889773.1\t8.95063706554687\t6.906754778648663\tTrue\t\
      True\tPredicted novel defense gene\tDS-30"
    notes: The final Science supplementary Table S6 maps working_id ABC3 to DS_name
      DS-30, marks the cloned transcriptional unit as defensive, and records four
      product accessions in NZ_QOYR01000032.1 positions 1906-4621.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
    snippet: "SECphi27\t5.1\tpLAND\t24-05-31_EV_CRDO_BRNT_ABC3.png\t100000000\tABC3\t\
      24-05-31\t24-05-31_EV_CRDO_BRNT_ABC3.png\t5\t1\tY\t100000\t3\t\tTrue\t\tM9\t\
      30"
    notes: The final Science supplementary Table S7 Systems sheet reports an ABC3
      assay row with a SECphi27 phage readout, a smaller-plaque-size call, and a -log(EOP)
      value of 3.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
    snippet: "ABC3\tDS-30\tTrue"
    notes: The final Science supplementary Table S8 maps ABC3 to replicated display
      name DS-30.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
    snippet: "ABC3\t2.0\t182.0\tWP_000534626.1\tABC-3C C-term\tPF20275.3\tCTD10 ;\
      \ C-terminal domain 10 of the ABC-three component (ABC-3C) systems\thhpred_7102862.hhr\t\
      26.0\t176.0\t1.0\t2024-07-30 00:00:00"
    notes: The final Science supplementary Table S8 reports a high-probability CTD10
      HHpred hit for ABC3 protein 2, WP_000534626.1.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
    snippet: "ABC3\t3.0\t77.0\tWP_000628292.1\tABC-3C Mid\tPF20293.3\tMC6 ; ABC-three\
      \ component (ABC-3C) system Middle Component 6\thhpred_3406401.hhr\t1.0\t75.0\t\
      1.0\t2024-07-30 00:00:00"
    notes: The final Science supplementary Table S8 reports a high-probability MC6
      HHpred hit for ABC3 protein 3, WP_000628292.1.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
    snippet: "ABC3\t4.0\t565.0\tWP_087889773.1\tABC ATPase\t5XEI_A\tChromosome partition\
      \ protein Smc; Condensin, Smc, head domain, ABC-ATPase,\thhpred_6417130.hhr\t\
      1.0\t540.0\t1.0\t2024-07-30 00:00:00"
    notes: The final Science supplementary Table S8 reports a high-probability ABC
      ATPase HHpred hit for ABC3 protein 4, WP_087889773.1.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
    snippet: "ABC3\t1.0\t86.0\tWP_001553315.1\tFtsL\t8HHF_L\tCell division protein\
      \ FtsL; Bacterial cell division, divisome, FtsB, FtsL, FtsQ, FtsBLQ, membrane\
      \ protein complex, heterotrimer, MEMBRANE PROTEIN\thhpred_2515914.hhr\t2.0\t\
      53.0\t0.942\t2024-07-30 00:00:00"
    notes: The final Science supplementary Table S8 reports an FtsL HHpred hit for
      ABC3 protein 1, WP_001553315.1.
  - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
    snippet: '| Lamassu-Fam__Lmu_DS-30A                          |                                                  |
      Lamassu-Fam            | Custom                  | 50     |'
    notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__Lmu_DS-30A
      as a custom Lamassu-Fam profile.
  - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
    snippet: '| Lamassu-Fam__LmuA_DS-30B                         |                                                  |
      Lamassu-Fam            | Custom                  | 60     |'
    notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuA_DS-30B
      as a custom Lamassu-Fam profile.
  - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
    snippet: '| Lamassu-Fam__LmuB_DS-30D                         |                                                  |
      Lamassu-Fam            | Custom                  | 150    |'
    notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuB_DS-30D
      as a custom Lamassu-Fam profile.
  - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
    snippet: '| Lamassu-Fam__LmuC_DS-30C                         |                                                  |
      Lamassu-Fam            | Custom                  | 20     |'
    notes: The pinned DefenseFinder HMM inventory records Lamassu-Fam__LmuC_DS-30C
      as a custom Lamassu-Fam profile.
  - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv
    notes: The pinned DefenseFinder rules table contains generic Lamassu-Fam subtype
      rows, but no DS-30-specific model row or DS-30 custom-profile criteria.
  attaches_to:
  - causal_graphs#ds_30_locus_reduces_phage_plaquing
  posed_by: codex
  posed_date: '2026-09-29'
curation_history:
- timestamp: '2026-09-29T17:57:26Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted DS-30 system as a DOI-backed GENOMICS TraitRecord under Lamassu
    system after an ignored-and-hidden duplicate review found no exact live TraitMech,
    METPO, history, or prior proposal record; kept the graph at ABC3 transcriptional-unit
    level because native host breadth, exact DS-30 profile-to-component mapping, Lamassu
    subtype chemistry, LmuC requirement, complete phage breadth, and DefenseFinder
    rule-level criteria remain unresolved, and proposals/metpo_traitmech_v347 reserves
    the replacement placeholder.
  llm_assisted: true
- timestamp: '2026-09-29T17:57:27Z'
  curator: codex
  action: REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP
  changes: Reviewed DS-30 system canonical_examples and left them empty because DeWeirdt
    et al. directly support cloned ABC3 assays in E. coli MG1655 and Lamassu-Fam DS-30
    profile rows, but not a direct named native microbial isolate exemplar with experimentally
    verified endogenous DS-30 activity. No paid research was used.
  llm_assisted: true