identifier: traitmech:000459
label: DS-37 system
definition: A phage defense system in which an organism possesses the single-gene
  DefensePredictor-discovered system 37 locus cataloged as working transcriptional
  unit SC24 and whose plasmid expression in E. coli MG1655 reduced bacteriophage plaquing.
definition_source: DOI:10.1126/science.adv7924
trait_category: GENOMICS
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- traitmech:000209
synonyms:
- synonym_text: DS-37
  synonym_type: EXACT_SYNONYM
  source: DOI:10.1126/science.adv7924
- synonym_text: SC24
  synonym_type: RELATED_SYNONYM
  source: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
- synonym_text: DS-37__DS-37
  synonym_type: RELATED_SYNONYM
  source: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
evidence:
- reference: DOI:10.1126/science.adv7924
  snippet: To test for anti-phage defense, we placed each TU with its predicted native
    promoter region on a low-copy number plasmid in E. coli MG1655 and challenged
    these strains with a panel of 24 diverse E. coli phages (Fig. 3; fig. S2). In
    total, 42 (45% of 94) of the cloned TUs produced smaller plaque sizes or reduced
    the efficiency of plating (EOP) at least ten-fold relative to an empty vector
    control strain
  notes: DeWeirdt et al. experimentally validated 42 predicted transcriptional units
    as phage-defense systems in E. coli.
- reference: DOI:10.1126/science.adv7924
  snippet: We refer to these validated TUs as DefensePredictor discovered systems
    (DSs), with genes in multi-gene TUs denoted by an alphabetical suffix, e.g., DS-8A
    is the first gene of DS-8.
  notes: DeWeirdt et al. name validated transcriptional units as DefensePredictor
    discovered systems.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
  snippet: "SC24\tNZ_QOXM01000003.1\tGCF_003334665.1\t+\tTrue\tFalse\tFalse\tTrue\t\
    DefensePredictor hits\t26984\t28375\thypothetical protein\tWP_000982358.1\t6.427335289528256\t\
    -2.24248116892434\tTrue\tFalse\tPredicted novel defense gene\tDS-37"
  notes: The final Science supplementary Table S6 maps working_id SC24 to DS_name
    DS-37, marks the cloned transcriptional unit as defensive, and records NZ_QOXM01000003.1
    positions 26984-28375 with product accession WP_000982358.1.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
  snippet: "Bas1\t6.1\tpLAND\t24-07-17_MVB1_VPUS_VAME_EV.png\t3000000\tSC24\t24-07-24\t\
    24-07-24_TRNA_MF4L_SC24_EV.png\t0\t1\t\t1\t6.477121254719663\t\t\tTrue\tLB\t37"
  notes: The final Science supplementary Table S7 reports an SC24 assay row with a
    Bas1 phage readout and a -log(EOP) value of 6.477.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
  snippet: "Bas9\t6.1\tpLAND\t24-07-17_MVB1_VPUS_VAME_EV.png\t600000000\tSC24\t24-07-24\t\
    24-07-24_TRNA_MF4L_SC24_EV.png\t3\t10\t\t10000\t4.778151250383644\t\t\tTrue\t\
    LB\t37"
  notes: The final Science supplementary Table S7 reports an SC24 assay row with a
    Bas9 phage readout and a -log(EOP) value of 4.778.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
  snippet: "SC24\tDS-37\tTrue"
  notes: The final Science supplementary Table S8 maps SC24 to replicated display
    name DS-37.
- reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
  snippet: "SC24\t1.0\t463.0\tWP_000982358.1\tPDDEXK\tPF08378.16\tNERD ; Nuclease-related\
    \ domain\thhpred_2278954.hhr\t154.0\t204.0\t0.47\t2024-07-29 00:00:00\t210-212\t\
    199.0"
  notes: The final Science supplementary Table S8 reports a low-probability PDDEXK
    HHpred lead for WP_000982358.1 in SC24; this first-pass record records it as source
    context without resolving DS-37 molecular activity.
- reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
  snippet: '| DS-37 | 10\.1101/2025\.01\.08\.631726 | DefensePredictor: A machine
    learning model to discover novel prokaryotic immune systems | '
  notes: The pinned DefenseFinder article registry maps the DS-37 source key to the
    preprint DOI for the DeWeirdt et al. DefensePredictor study, which has since been
    published in Science.
- reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
  snippet: '| DS-37__DS-37                                     |                                                  |
    DS-37                  | Custom                  | 300    |'
  notes: The pinned DefenseFinder HMM inventory records DS-37__DS-37 as a custom DS-37
    profile.
causal_graphs:
- graph_id: ds_37_locus_reduces_phage_plaquing
  title: DS-37 locus reduces bacteriophage plaquing
  description: Conservative system-level sketch linking the single-gene DS-37 locus
    to reduced bacteriophage plaquing without resolving DS-37 component function or
    molecular output.
  scope_status: NONMECHANISTIC
  scope_notes: The graph captures DS-37 as the validated SC24 transcriptional unit
    with one product accession, one low-probability PDDEXK HHpred row for WP_000982358.1
    in Table S8, and one DefenseFinder DS-37 profile row. It does not assert exact
    profile-to-protein correspondence, PDDEXK domain interpretation, nuclease chemistry,
    native host breadth, DS-37 molecular output, complete phage breadth, or DefenseFinder
    rule-level detection criteria.
  nodes:
  - node_id: ds_37_locus
    label: DS-37 locus
    node_type: GENETIC_ELEMENT
    description: A single-gene DefensePredictor-discovered system 37 locus represented
      in the pinned DefenseFinder HMM inventory by one DS-37 custom profile.
  - node_id: reduced_phage_plaquing
    label: reduced bacteriophage plaquing
    node_type: BIOLOGICAL_PROCESS
    description: Reduced bacteriophage plaquing in cells carrying cloned SC24.
  - node_id: ds_37_system_trait
    label: DS-37 system
    node_type: TRAIT
    grounding: traitmech:000459
    description: Possession of a genome-encoded DS-37 phage-defense system.
  - node_id: phage_defense_system
    label: phage defense system
    node_type: TRAIT
    grounding: traitmech:000209
    description: Possession of one or more genome-encoded immune systems that inhibit
      bacteriophage infection.
  edges:
  - subject: ds_37_locus
    predicate: contributes to
    predicate_id: RO:0002326
    object: reduced_phage_plaquing
    description: The DS-37/SC24 locus contributes to reduced bacteriophage plaquing
      in heterologous E. coli MG1655 plasmid-expression assays.
    evidence:
    - reference: DOI:10.1126/science.adv7924
      snippet: To test for anti-phage defense, we placed each TU with its predicted
        native promoter region on a low-copy number plasmid in E. coli MG1655 and
        challenged these strains with a panel of 24 diverse E. coli phages (Fig. 3;
        fig. S2). In total, 42 (45% of 94) of the cloned TUs produced smaller plaque
        sizes or reduced the efficiency of plating (EOP) at least ten-fold relative
        to an empty vector control strain
      notes: DeWeirdt et al. describe the plasmid-based phage challenge used to validate
        predicted transcriptional units.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
      snippet: "SC24\tNZ_QOXM01000003.1\tGCF_003334665.1\t+\tTrue\tFalse\tFalse\t\
        True\tDefensePredictor hits\t26984\t28375\thypothetical protein\tWP_000982358.1\t\
        6.427335289528256\t-2.24248116892434\tTrue\tFalse\tPredicted novel defense\
        \ gene\tDS-37"
      notes: The final Science supplementary Table S6 maps working_id SC24 to DS_name
        DS-37, marks the cloned transcriptional unit as defensive, and records NZ_QOXM01000003.1
        positions 26984-28375 with product accession WP_000982358.1.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
      snippet: "Bas1\t6.1\tpLAND\t24-07-17_MVB1_VPUS_VAME_EV.png\t3000000\tSC24\t\
        24-07-24\t24-07-24_TRNA_MF4L_SC24_EV.png\t0\t1\t\t1\t6.477121254719663\t\t\
        \tTrue\tLB\t37"
      notes: The final Science supplementary Table S7 reports an SC24 assay row with
        a Bas1 phage readout and a -log(EOP) value of 6.477.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
      snippet: "Bas9\t6.1\tpLAND\t24-07-17_MVB1_VPUS_VAME_EV.png\t600000000\tSC24\t\
        24-07-24\t24-07-24_TRNA_MF4L_SC24_EV.png\t3\t10\t\t10000\t4.778151250383644\t\
        \t\tTrue\tLB\t37"
      notes: The final Science supplementary Table S7 reports an SC24 assay row with
        a Bas9 phage readout and a -log(EOP) value of 4.778.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
      snippet: "SC24\tDS-37\tTrue"
      notes: The final Science supplementary Table S8 maps SC24 to replicated display
        name DS-37.
    - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
      snippet: '| DS-37__DS-37                                     |                                                  |
        DS-37                  | Custom                  | 300    |'
      notes: The pinned DefenseFinder HMM inventory records DS-37__DS-37 as a custom
        DS-37 profile.
  - subject: reduced_phage_plaquing
    predicate: confers
    predicate_id: METPO:2007700
    object: ds_37_system_trait
    description: DS-37-mediated phage plaquing reduction realizes the DS-37 system
      trait.
    evidence:
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
      snippet: "SC24\tNZ_QOXM01000003.1\tGCF_003334665.1\t+\tTrue\tFalse\tFalse\t\
        True\tDefensePredictor hits\t26984\t28375\thypothetical protein\tWP_000982358.1\t\
        6.427335289528256\t-2.24248116892434\tTrue\tFalse\tPredicted novel defense\
        \ gene\tDS-37"
      notes: The final Science supplementary Table S6 maps working_id SC24 to DS_name
        DS-37, marks the cloned transcriptional unit as defensive, and records NZ_QOXM01000003.1
        positions 26984-28375 with product accession WP_000982358.1.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
      snippet: "Bas1\t6.1\tpLAND\t24-07-17_MVB1_VPUS_VAME_EV.png\t3000000\tSC24\t\
        24-07-24\t24-07-24_TRNA_MF4L_SC24_EV.png\t0\t1\t\t1\t6.477121254719663\t\t\
        \tTrue\tLB\t37"
      notes: The final Science supplementary Table S7 reports an SC24 assay row with
        a Bas1 phage readout and a -log(EOP) value of 6.477.
    - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
      snippet: "Bas9\t6.1\tpLAND\t24-07-17_MVB1_VPUS_VAME_EV.png\t600000000\tSC24\t\
        24-07-24\t24-07-24_TRNA_MF4L_SC24_EV.png\t3\t10\t\t10000\t4.778151250383644\t\
        \t\tTrue\tLB\t37"
      notes: The final Science supplementary Table S7 reports an SC24 assay row with
        a Bas9 phage readout and a -log(EOP) value of 4.778.
    - reference: DOI:10.1126/science.adv7924
      snippet: We refer to these validated TUs as DefensePredictor discovered systems
        (DSs), with genes in multi-gene TUs denoted by an alphabetical suffix, e.g.,
        DS-8A is the first gene of DS-8.
      notes: The DS nomenclature is used for DefensePredictor discovered systems.
  - subject: ds_37_system_trait
    predicate: is a
    predicate_id: rdfs:subClassOf
    object: phage_defense_system
    description: DS-37 system possession is a phage-defense-system trait.
    evidence:
    - reference: DOI:10.1126/science.adv7924
      snippet: We refer to these validated TUs as DefensePredictor discovered systems
        (DSs), with genes in multi-gene TUs denoted by an alphabetical suffix, e.g.,
        DS-8A is the first gene of DS-8.
      notes: DeWeirdt et al. name validated TUs as DefensePredictor discovered systems.
    - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
      snippet: '| DS-37 | 10\.1101/2025\.01\.08\.631726 | DefensePredictor: A machine
        learning model to discover novel prokaryotic immune systems | '
      notes: The pinned DefenseFinder article registry maps the DS-37 source key to
        the preprint DOI for the DeWeirdt et al. DefensePredictor study, which has
        since been published in Science.
discussions:
- discussion_id: ds-37-defensefinder-model-gap
  prompt: Resolve DS-37 native host breadth, exact component activity, profile-to-protein
    mapping, low-probability PDDEXK HHpred-domain interpretation, nuclease chemistry,
    complete phage breadth, molecular output, and rule-level DefenseFinder criteria
    before minting narrower DS-37 mechanism children.
  kind: KNOWLEDGE_GAP
  status: OPEN
  rationale: DeWeirdt et al. support DS-37 as the defensive SC24 transcriptional unit
    and final Science Tables S6/S7/S8 map it to one product accession, Bas1 and Bas9
    phage readouts, display name DS-37, and one low-probability PDDEXK HHpred row.
    The pinned DefenseFinder HMM inventory records one DS-37 custom profile row. The
    pinned rules table has no DS-37 row, and the first-pass record does not resolve
    native host breadth, complete phage breadth, direct profile-to-protein correspondence,
    PDDEXK domain interpretation, nuclease chemistry, molecular output, or endogenous
    DS-37 activity.
  evidence:
  - reference: DOI:10.1126/science.adv7924
    snippet: We refer to these validated TUs as DefensePredictor discovered systems
      (DSs), with genes in multi-gene TUs denoted by an alphabetical suffix, e.g.,
      DS-8A is the first gene of DS-8.
    notes: DeWeirdt et al. name validated TUs as DSs.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S6.xlsx
    snippet: "SC24\tNZ_QOXM01000003.1\tGCF_003334665.1\t+\tTrue\tFalse\tFalse\tTrue\t\
      DefensePredictor hits\t26984\t28375\thypothetical protein\tWP_000982358.1\t\
      6.427335289528256\t-2.24248116892434\tTrue\tFalse\tPredicted novel defense gene\t\
      DS-37"
    notes: The final Science supplementary Table S6 maps working_id SC24 to DS_name
      DS-37, marks the cloned transcriptional unit as defensive, and records NZ_QOXM01000003.1
      positions 26984-28375 with product accession WP_000982358.1.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
    snippet: "Bas1\t6.1\tpLAND\t24-07-17_MVB1_VPUS_VAME_EV.png\t3000000\tSC24\t24-07-24\t\
      24-07-24_TRNA_MF4L_SC24_EV.png\t0\t1\t\t1\t6.477121254719663\t\t\tTrue\tLB\t\
      37"
    notes: The final Science supplementary Table S7 reports an SC24 assay row with
      a Bas1 phage readout and a -log(EOP) value of 6.477.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S7.xlsx
    snippet: "Bas9\t6.1\tpLAND\t24-07-17_MVB1_VPUS_VAME_EV.png\t600000000\tSC24\t\
      24-07-24\t24-07-24_TRNA_MF4L_SC24_EV.png\t3\t10\t\t10000\t4.778151250383644\t\
      \t\tTrue\tLB\t37"
    notes: The final Science supplementary Table S7 reports an SC24 assay row with
      a Bas9 phage readout and a -log(EOP) value of 4.778.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
    snippet: "SC24\tDS-37\tTrue"
    notes: The final Science supplementary Table S8 maps SC24 to replicated display
      name DS-37.
  - reference: https://pmc-oa-opendata.s3.amazonaws.com/PMC13092281.1/NIHMS2163519-supplement-Table_S8.xlsx
    snippet: "SC24\t1.0\t463.0\tWP_000982358.1\tPDDEXK\tPF08378.16\tNERD ; Nuclease-related\
      \ domain\thhpred_2278954.hhr\t154.0\t204.0\t0.47\t2024-07-29 00:00:00\t210-212\t\
      199.0"
    notes: The final Science supplementary Table S8 reports a low-probability PDDEXK
      HHpred lead for WP_000982358.1 in SC24; this first-pass record records it as
      source context without resolving DS-37 molecular activity.
  - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
    snippet: '| DS-37 | 10\.1101/2025\.01\.08\.631726 | DefensePredictor: A machine
      learning model to discover novel prokaryotic immune systems | '
    notes: The pinned DefenseFinder article registry maps the DS-37 source key to
      the preprint DOI for the DeWeirdt et al. DefensePredictor study, which has since
      been published in Science.
  - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
    snippet: '| DS-37__DS-37                                     |                                                  |
      DS-37                  | Custom                  | 300    |'
    notes: The pinned DefenseFinder HMM inventory records DS-37__DS-37 as a custom
      DS-37 profile.
  - reference: https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv
    notes: The pinned DefenseFinder rules table does not list DS-37, leaving rule-level
      detection criteria unresolved.
  attaches_to:
  - causal_graphs#ds_37_locus_reduces_phage_plaquing
  posed_by: codex
  posed_date: '2026-09-29'
curation_history:
- timestamp: '2026-09-29T10:28:09Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted DS-37 system as a DOI-backed GENOMICS TraitRecord under phage defense
    system after an ignored-and-hidden duplicate review found no exact live TraitMech,
    METPO, history, or prior proposal record; kept the graph at SC24 transcriptional-unit
    level because the low-probability PDDEXK row and rule rows remain unresolved,
    and proposals/metpo_traitmech_v336 reserves the replacement placeholder.
  llm_assisted: true
- timestamp: '2026-09-29T10:28:10Z'
  curator: codex
  action: REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP
  changes: Reviewed DS-37 system canonical_examples and left them empty because DeWeirdt
    et al. directly support cloned SC24 assays in E. coli MG1655 and a DefenseFinder
    DS-37 model, but not a direct named native microbial isolate exemplar with experimentally
    verified endogenous DS-37 activity. No paid research was used.
  llm_assisted: true