identifier: traitmech:000526 label: MksBEFG system definition: A Wadjet system in which an organism possesses an MksBEFG derivative SMC locus encoding an MksBEF ATPase core and an MksG nuclease that degrades plasmid DNA. definition_source: DOI:10.1093/nar/gkad130 trait_category: GENOMICS term_kind: CLASS mapping_status: PROPOSED parent_traits: - traitmech:000218 synonyms: - synonym_text: MksBEFG synonym_type: RELATED_SYNONYM source: DOI:10.1093/nar/gkad130 evidence: - reference: DOI:10.1093/nar/gkad130 snippet: The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG notes: Weiss et al. describe MksBEFG as a bacterial plasmid-defense system and identify MksG as its executing nuclease component. - reference: DOI:10.1093/nar/gkad130 snippet: MksG is a nuclease that degrades plasmid DNA notes: Weiss et al. support plasmid-DNA degradation by the MksG nuclease of the MksBEFG system. - reference: DOI:10.1093/nar/gkad130 snippet: Introduction of plasmids results in an increase in DNA bound MksG, indicating an activation of the system in vivo notes: Weiss et al. support plasmid-responsive activation of the Corynebacterium glutamicum MksBEFG system in vivo. - reference: DOI:10.1093/nar/gkad130 snippet: The MksBEF subunits exhibit an ATPase cycle in vitro notes: Weiss et al. support ATPase cycling by the C. glutamicum MksBEF core. - reference: DOI:10.1016/j.molcel.2022.11.015 snippet: Wadjet systems (JetABCD/MksBEFG/EptABCD) are derivative SMC complexes with roles in bacterial immunity against selfish DNA notes: Liu et al. support MksBEFG as a Wadjet-family derivative SMC complex related to JetABCD and EptABCD. canonical_examples: - taxon_id: NCBITaxon:1718 taxon_label: Corynebacterium glutamicum note: Weiss et al. investigated the Corynebacterium glutamicum MksBEFG complex and showed that plasmid introduction increased DNA-bound MksG in vivo. reference: DOI:10.1093/nar/gkad130 causal_graphs: - graph_id: mksbefg_degrades_plasmid_dna title: MksBEFG systems degrade plasmid DNA description: Conservative system-level sketch linking MksBEFG loci to MksG-dependent plasmid DNA degradation and Wadjet system possession. scope_status: NONMECHANISTIC scope_notes: The graph captures the MksBEFG subfamily at locus and plasmid-degradation-output level without asserting the exact MksBEF ATPase-to-MksG coupling step, plasmid substrate breadth, cellular MksG loading path, or accession-level protein examples. nodes: - node_id: mksbefg_locus label: MksBEFG locus node_type: GENETIC_ELEMENT description: A Wadjet-family plasmid-defense locus encoding MksB, MksE, MksF, and MksG components. - node_id: mksbef_atpase_cycle label: MksBEF ATPase cycle node_type: BIOLOGICAL_PROCESS description: ATPase cycling by the MksB, MksE, and MksF subunits of the MksBEFG complex. - node_id: mksg_plasmid_dna_degradation label: MksG plasmid DNA degradation node_type: BIOLOGICAL_PROCESS description: Degradation of plasmid DNA by the MksG nuclease component of the MksBEFG system. - node_id: plasmid_transformation label: plasmid transformation node_type: BIOLOGICAL_PROCESS description: Introduction and establishment of exogenous plasmid DNA in a bacterial cell. - node_id: mksbefg_system_trait label: MksBEFG system node_type: TRAIT grounding: traitmech:000526 description: Possession of a genome-encoded MksBEFG anti-plasmid defense system. - node_id: wadjet_system_trait label: Wadjet system node_type: TRAIT grounding: traitmech:000218 description: Possession of a genome-encoded Wadjet anti-plasmid defense locus. edges: - subject: mksbefg_locus predicate: contributes to predicate_id: RO:0002326 object: mksbef_atpase_cycle description: MksBEFG loci encode the MksBEF ATPase core. evidence: - reference: DOI:10.1093/nar/gkad130 snippet: The MksBEF subunits exhibit an ATPase cycle in vitro notes: Weiss et al. support ATPase cycling by the C. glutamicum MksBEF core. - subject: mksbefg_locus predicate: contributes to predicate_id: RO:0002326 object: mksg_plasmid_dna_degradation description: MksBEFG loci encode the MksG nuclease that degrades plasmid DNA. evidence: - reference: DOI:10.1093/nar/gkad130 snippet: The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG notes: Weiss et al. describe MksBEFG as a bacterial plasmid-defense system and identify MksG as its executing nuclease component. - reference: DOI:10.1093/nar/gkad130 snippet: MksG is a nuclease that degrades plasmid DNA notes: Weiss et al. support plasmid-DNA degradation by the MksG nuclease of the MksBEFG system. - subject: mksg_plasmid_dna_degradation predicate: mitigates predicate_id: METPO:2007407 object: plasmid_transformation description: MksG-dependent plasmid DNA degradation restricts incoming plasmid DNA. evidence: - reference: DOI:10.1093/nar/gkad130 snippet: MksG is a nuclease that degrades plasmid DNA notes: Weiss et al. support plasmid-DNA degradation by the MksG nuclease of the MksBEFG system. - reference: DOI:10.1093/nar/gkad130 snippet: Introduction of plasmids results in an increase in DNA bound MksG, indicating an activation of the system in vivo notes: Weiss et al. support plasmid-responsive activation of the Corynebacterium glutamicum MksBEFG system in vivo. - subject: mksg_plasmid_dna_degradation predicate: confers predicate_id: METPO:2007700 object: mksbefg_system_trait description: Plasmid DNA degradation realizes the MksBEFG anti-plasmid defense trait. evidence: - reference: DOI:10.1093/nar/gkad130 snippet: The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG notes: Weiss et al. describe MksBEFG as a bacterial plasmid-defense system and identify MksG as its executing nuclease component. - subject: mksbefg_system_trait predicate: is a predicate_id: rdfs:subClassOf object: wadjet_system_trait description: MksBEFG system possession is a Wadjet system trait. evidence: - reference: DOI:10.1016/j.molcel.2022.11.015 snippet: Wadjet systems (JetABCD/MksBEFG/EptABCD) are derivative SMC complexes with roles in bacterial immunity against selfish DNA notes: Liu et al. support MksBEFG as a Wadjet-family derivative SMC complex related to JetABCD and EptABCD. discussions: - discussion_id: mksbefg-activation-and-substrate-gap prompt: Resolve MksBEFG plasmid substrate breadth, MksBEF ATPase-to-MksG coupling, MksG activation state, and accession-level protein examples before minting narrower MksBEFG mechanism or component traits. kind: KNOWLEDGE_GAP status: OPEN rationale: Weiss et al. support MksBEFG as a bacterial plasmid-defense system with an MksBEF ATPase core and MksG plasmid-DNA-degradation nuclease, and Liu et al. place MksBEFG in the broader Wadjet derivative-SMC family. This first-pass record leaves exact plasmid substrate breadth, MksBEF-to-MksG coupling, cellular MksG loading, and accession-level component examples unresolved. evidence: - reference: DOI:10.1093/nar/gkad130 snippet: The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG notes: Weiss et al. describe MksBEFG as a bacterial plasmid-defense system and identify MksG as its executing nuclease component. - reference: DOI:10.1093/nar/gkad130 snippet: MksG is a nuclease that degrades plasmid DNA notes: Weiss et al. support plasmid-DNA degradation by the MksG nuclease of the MksBEFG system. - reference: DOI:10.1093/nar/gkad130 snippet: Introduction of plasmids results in an increase in DNA bound MksG, indicating an activation of the system in vivo notes: Weiss et al. support plasmid-responsive activation of the Corynebacterium glutamicum MksBEFG system in vivo. - reference: DOI:10.1093/nar/gkad130 snippet: The MksBEF subunits exhibit an ATPase cycle in vitro notes: Weiss et al. support ATPase cycling by the C. glutamicum MksBEF core. - reference: DOI:10.1016/j.molcel.2022.11.015 snippet: Wadjet systems (JetABCD/MksBEFG/EptABCD) are derivative SMC complexes with roles in bacterial immunity against selfish DNA notes: Liu et al. support MksBEFG as a Wadjet-family derivative SMC complex related to JetABCD and EptABCD. attaches_to: - causal_graphs#mksbefg_degrades_plasmid_dna posed_by: codex posed_date: '2026-10-01' curation_history: - timestamp: '2026-10-01T21:15:00Z' curator: codex action: MINTED_TRAITMECH_ID changes: Minted MksBEFG system as a DOI-backed GENOMICS TraitRecord under the Wadjet system parent after an ignored-and-hidden duplicate review found no exact same-scope live TraitMech, METPO, history, or prior proposal record; the replacement placeholder is reserved in proposals/metpo_traitmech_v403. llm_assisted: true