identifier: METPO:2000040
label: does not use as nitrogen source
definition: An OBJECT_PROPERTY asserting that an organism does NOT use a given chemical
  as a source of nitrogen for biosynthesis. Negation companion of 'uses as nitrogen
  source'.
definition_source: PMID:21413255
trait_category: METABOLISM
term_kind: OBJECT_PROPERTY
mapping_status: REVIEWED
parent_traits:
- METPO:2000001
synonyms:
- synonym_text: nitrogen source
  synonym_type: RELATED_SYNONYM
  source: metpo.owl
domain: https://w3id.org/metpo/1000525
range_: http://purl.obolibrary.org/obo/CHEBI_51143
evidence:
- reference: PMID:21413255
  snippet: nitrogen source
  notes: Microbial-physiology reference supports 'does not use as nitrogen source'
    as a canonical predicate composed with a CHEBI-class chemical object at assertion
    time.
curation_history:
- timestamp: '2026-05-05T01:35:46.969885+00:00'
  curator: seed_from_metpo
  action: SEEDED_FROM_METPO
  changes: imported from data/raw/metpo.owl (OBJECT_PROPERTY)
  llm_assisted: false
- timestamp: '2026-05-10T00:00:00-07:00'
  curator: codex
  action: DEPRECATED_PROPERTY_RECORD
  changes: Deprecated generic chemical-use relation carrier; later replacement should
    use more specific trait records that combine the relation with the chemical, metabolic
    role, growth condition, and positive or negative usage context.
  llm_assisted: true
- timestamp: '2026-05-16T08:50:00-07:00'
  curator: claude
  action: UNDEPRECATED_AS_COMPOSITION_PRIMITIVE
  changes: 'Restored from DEPRECATED to REVIEWED under the predicate+class composition
    pattern (intended use: <organism> + this predicate + CHEBI chemical class). Added
    DOI/PMID-backed definition, evidence, and explicit domain/range slots.'
  llm_assisted: true
- timestamp: '2026-05-16T09:35:00-07:00'
  curator: claude
  action: TIGHTENED_RANGE_TO_CHEBI_SUBCLASS
  changes: 'Narrowed range_ from http://purl.obolibrary.org/obo/CHEBI_24431 to http://purl.obolibrary.org/obo/CHEBI_51143
    (''nitrogen molecular entity''). The earlier CHEBI:24431 root was used as a safe
    default during bulk un-deprecation (PR #54); the more specific CHEBI subclass
    better reflects the predicate''s intended substrates.'
  llm_assisted: true