identifier: METPO:1000889 label: cell width medium definition: A cell-width phenotype in which the shorter cell dimension lies approximately between 0.65 and 0.9 micrometers. definition_source: DOI:10.1146/annurev-cellbio-101011-155745 trait_category: MORPHOLOGY term_kind: CLASS mapping_status: REVIEWED parent_traits: - METPO:1000882 synonyms: - synonym_text: W_0.65_0.9 synonym_type: RELATED_SYNONYM source: metpo.owl evidence: - reference: DOI:10.1146/annurev-cellbio-101011-155745 snippet: MreB-directed peptidoglycan synthesis notes: Supports the MreB/Rod-complex set-point producing typical rod widths in the 0.65–0.9 μm range. causal_graphs: - graph_id: cell_width_medium_typical_rod title: Medium cell-width typical rod description: DOI-backed graph linking MreB-directed lateral wall synthesis under standard growth conditions to typical rod radii (0.65–0.9 μm). nodes: - node_id: cell_width_medium_trait label: cell width medium node_type: TRAIT grounding: METPO:1000889 description: Cell width approximately 0.65–0.9 μm. - node_id: cell_width_trait label: cell width node_type: TRAIT grounding: METPO:1000882 description: Regulated shorter cellular dimension. - node_id: rod_complex_pg label: Rod-complex peptidoglycan synthesis node_type: BIOLOGICAL_PROCESS description: MreB-organized lateral peptidoglycan assembly. - node_id: standard_growth_conditions label: standard growth conditions node_type: ENVIRONMENTAL_FACTOR description: Routine laboratory growth conditions producing typical bacterial rod widths. - node_id: roda_pbp2_complex label: RodA-PBP2 complex node_type: GENE_OR_PROTEIN description: Elongasome SEDS-bPBP synthase pair (RodA with PBP2) driving lateral peptidoglycan synthesis. - node_id: pg_polymerization_crosslinking label: peptidoglycan polymerization and crosslinking node_type: BIOLOGICAL_PROCESS description: Glycan strand polymerization and peptide crosslinking during cell-wall elongation. - node_id: mrec_protein label: MreC node_type: GENE_OR_PROTEIN description: Membrane-associated Rod-complex protein that activates PBP2. grounding: UniProtKB:A0A1L9R356 - node_id: pbp2_protein label: PBP2 node_type: GENE_OR_PROTEIN description: Bifunctional class B penicillin-binding protein (transpeptidase) of the elongasome. grounding: UniProtKB:A0A2D2D7X6 - node_id: mred_protein label: MreD node_type: GENE_OR_PROTEIN description: Rod-complex membrane protein that modulates PBP2 activity in balance with MreC. grounding: UniProtKB:A0A0H2VI68 - node_id: apbp_proteins label: class A penicillin-binding proteins (aPBPs) node_type: GENE_OR_PROTEIN description: Bifunctional glycosyltransferase/transpeptidase peptidoglycan synthases that influence cell diameter. edges: - subject: standard_growth_conditions predicate: enables object: rod_complex_pg description: Standard growth regimes support typical Rod-complex activity. evidence: - reference: DOI:10.1146/annurev-cellbio-101011-155745 snippet: MreB-directed peptidoglycan synthesis notes: Supports MreB-organized PG synthesis under standard conditions. predicate_id: RO:0002327 - subject: rod_complex_pg predicate: produces object: cell_width_medium_trait description: Rod-complex PG synthesis sustains a typical rod radius. evidence: - reference: DOI:10.1038/nrmicro3088 snippet: rod-shape is maintained notes: Supports lateral PG assembly as the producer of typical rod radius. predicate_id: METPO:2000202 - subject: cell_width_medium_trait predicate: is a object: cell_width_trait description: Medium cell width is a quantitative bin of the cell-width phenotype. evidence: - reference: DOI:10.1146/annurev-cellbio-101011-155745 snippet: MreB-directed peptidoglycan synthesis notes: Supports medium width as a value within the regulated cell-width distribution. predicate_id: rdfs:subClassOf - subject: roda_pbp2_complex predicate: positively regulates object: pg_polymerization_crosslinking description: Structural opening of the RodA-PBP2 complex couples and activates PG polymerization and crosslinking during elongation. evidence: - reference: DOI:10.1038/s41467-023-39037-9 notes: RodA-PBP2 undergoes dynamic exchange between closed and open states; structural opening couples activation of polymerization and crosslinking and is essential in vivo. - subject: pg_polymerization_crosslinking predicate: produces object: rod_complex_pg description: Elongasome polymerization/crosslinking constitutes the Rod-complex lateral peptidoglycan synthesis that sets rod radius. evidence: - reference: DOI:10.1038/s41467-023-39037-9 notes: Activation of polymerization and crosslinking is the elongation activity underlying Rod-complex lateral wall synthesis. predicate_id: METPO:2000202 - subject: mrec_protein predicate: activates object: pbp2_protein description: MreC interacts with PBP2 and stimulates its peptidoglycan polymerization and crosslinking activity. evidence: - reference: DOI:10.1002/mbo3.1385 notes: MreC interacts with PBP2 and is thought to cause a structural change in PBP2 and stimulate peptidoglycan polymerization and crosslinking. predicate_id: RO:0002213 - subject: mred_protein predicate: modulates activity of object: pbp2_protein description: MreD modulates PBP2 activity; the balance between MreC and MreD determines PBP2 activity. evidence: - reference: DOI:10.1002/mbo3.1385 notes: The balance between MreC and MreD determines the activity of PBP2 (curated as modulatory). - subject: pbp2_protein predicate: enables object: rod_complex_pg description: Activated PBP2 transpeptidation supports Rod-complex lateral peptidoglycan synthesis. evidence: - reference: DOI:10.1002/mbo3.1385 notes: PBP2 activity drives peptidoglycan polymerization and crosslinking of the Rod complex. predicate_id: RO:0002327 - subject: apbp_proteins predicate: regulates object: cell_width_trait description: Class A PBPs regulate cell diameter; loss of aPBPs yields shorter, wider cells. evidence: - reference: DOI:10.1038/s41467-023-41082-3 notes: aPBPs regulate cell diameter; strains lacking aPBPs were moderately but significantly shorter and wider. predicate_id: RO:0002211 curation_history: - timestamp: '2026-05-05T01:35:46.922158+00:00' curator: seed_from_metpo action: SEEDED_FROM_METPO changes: imported from data/raw/metpo.owl (CLASS) llm_assisted: false - timestamp: '2026-05-11T22:30:00-07:00' curator: claude action: CURATED_CAUSAL_GRAPH changes: Added DOI-backed causal graph linking MreB-directed Rod-complex peptidoglycan synthesis to medium cell width (0.65–0.9 μm). llm_assisted: true - timestamp: '2026-05-20T03:35:31Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 2 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (METPO:2000202×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-05-24T04:13:19Z' curator: claude action: RENAME_PREDICATE_LABELS changes: 'Renamed 1 causal-edge predicate label(s) to align with existing groundings: supports → enables ×1.' llm_assisted: true - timestamp: '2026-05-24T04:13:21Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 1 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002327×1). llm_assisted: true - timestamp: '2026-06-23T00:00:00Z' curator: claude action: ENRICH_CAUSAL_GRAPH changes: Added 6 evidence-backed generic edges (6 new nodes) from the deep-research report. llm_assisted: true - timestamp: '2026-06-24T17:21:13Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 4 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (METPO:2000202×1, RO:0002213×1, RO:0002327×1, RO:0002211×1). llm_assisted: true - timestamp: '2026-06-26T07:15:19Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 3 causal-node grounding field(s) via mappings/node_grounding.tsv (UniProtKB:A0A1L9R356×1, UniProtKB:A0A2D2D7X6×1, UniProtKB:A0A0H2VI68×1). llm_assisted: true