identifier: METPO:1000888 label: cell width small definition: A cell-width phenotype in which the shorter cell dimension lies approximately between 0.5 and 0.65 micrometers. definition_source: DOI:10.1146/annurev-cellbio-101011-155745 trait_category: MORPHOLOGY term_kind: CLASS mapping_status: REVIEWED parent_traits: - METPO:1000882 synonyms: - synonym_text: W_0.5_0.65 synonym_type: RELATED_SYNONYM source: metpo.owl evidence: - reference: DOI:10.1146/annurev-cellbio-101011-155745 snippet: MreB-directed peptidoglycan synthesis notes: Supports the MreB/Rod-complex set-point producing narrow rod widths in the 0.5–0.65 μm range. causal_graphs: - graph_id: cell_width_small_mreb_setpoint title: Small cell-width MreB-Rod set-point description: DOI-backed graph linking MreB-organized lateral wall synthesis to a narrow rod radius set-point (0.5–0.65 μm). nodes: - node_id: cell_width_small_trait label: cell width small node_type: TRAIT grounding: METPO:1000888 description: Cell width approximately 0.5–0.65 μm. - node_id: cell_width_trait label: cell width node_type: TRAIT grounding: METPO:1000882 description: Regulated shorter cellular dimension. - node_id: mreb_cytoskeleton label: MreB cytoskeleton node_type: GENE_OR_PROTEIN description: Actin-like MreB filaments organizing lateral cell-wall synthesis. - node_id: rod_complex_pg label: Rod-complex peptidoglycan synthesis node_type: BIOLOGICAL_PROCESS description: MreB-organized lateral peptidoglycan assembly. - node_id: mrec_protein label: MreC node_type: GENE_OR_PROTEIN description: Periplasmic Rod-complex protein that favors the open, active PBP2 state. grounding: UniProtKB:A0A1L9R356 - node_id: roda_pbp2_open label: RodA-PBP2 open conformation node_type: STATE description: Activated open conformation of the SEDS-bPBP RodA-PBP2 synthase complex. - node_id: pg_polymerization_crosslinking label: peptidoglycan polymerization and crosslinking node_type: BIOLOGICAL_PROCESS description: Glycan strand polymerization and peptide crosslinking output of the Rod complex. - node_id: rod_diameter_control label: rod width/diameter control node_type: CAPACITY description: Maintenance of a stable steady-state rod diameter (width set-point). - node_id: pg_hydrolase_activity label: peptidoglycan hydrolase activity node_type: MOLECULAR_FUNCTION description: Cell-wall hydrolase/autolysin activity that cleaves peptidoglycan. - node_id: magnesium_ion label: Mg2+ node_type: CHEMICAL description: Magnesium ion, an environmental modifier of cell-wall hydrolase activity. grounding: CHEBI:18420 edges: - subject: mreb_cytoskeleton predicate: enables object: rod_complex_pg description: MreB organizes lateral PG synthesis along the cylinder. evidence: - reference: DOI:10.1146/annurev-cellbio-101011-155745 snippet: MreB-directed peptidoglycan synthesis notes: Supports MreB as the lateral PG organizer. predicate_id: RO:0002327 - subject: rod_complex_pg predicate: produces object: cell_width_small_trait description: Rod-complex PG synthesis maintains a narrow cylinder radius. evidence: - reference: DOI:10.1038/nrmicro3088 snippet: rod-shape is maintained notes: Supports lateral PG assembly as the producer of the narrow rod radius. predicate_id: METPO:2000202 - subject: cell_width_small_trait predicate: is a object: cell_width_trait description: Small cell width is a quantitative bin of the cell-width phenotype. evidence: - reference: DOI:10.1146/annurev-cellbio-101011-155745 snippet: MreB-directed peptidoglycan synthesis notes: Supports small width as a value within the regulated cell-width distribution. predicate_id: rdfs:subClassOf - subject: mrec_protein predicate: activates object: roda_pbp2_open description: MreC favors the open, active conformation of the RodA-PBP2 synthase. evidence: - reference: DOI:10.1038/s41467-023-39037-9 notes: MreC favors the open, active PBP2 state; PBP2 suppressor alleles bypass defective MreC and increase polymerization activity (conserved Rod complex). predicate_id: RO:0002213 - subject: roda_pbp2_open predicate: increases object: pg_polymerization_crosslinking description: The open RodA-PBP2 state couples activation of polymerization and crosslinking. evidence: - reference: DOI:10.1038/s41467-023-39037-9 notes: Structural opening couples activation of polymerization and crosslinking; open-state mutants show stronger polymerization (conserved SEDS-bPBP systems). predicate_id: RO:0002213 - subject: pg_polymerization_crosslinking predicate: contributes to object: rod_complex_pg description: PG polymerization and crosslinking constitute the Rod-complex synthetic output. evidence: - reference: DOI:10.1038/s41467-023-39037-9 notes: Open-state activation links RodA-PBP2 conformation to peptidoglycan synthetic output of the Rod complex. predicate_id: RO:0002326 - subject: mreb_cytoskeleton predicate: required for object: rod_diameter_control description: MreB is a key determinant of rod shape and steady-state diameter/width control. evidence: - reference: DOI:10.1016/j.cell.2018.02.050 notes: MreB is a key determinant of rod shape and diameter; perturbing MreB changes steady-state diameter and width control (broad, foundational). - subject: rod_diameter_control predicate: produces object: cell_width_small_trait description: Stable diameter control maintains the narrow rod radius set-point. evidence: - reference: DOI:10.1016/j.cell.2018.02.050 notes: Width/diameter control governs the steady-state rod radius producing the narrow width phenotype. predicate_id: METPO:2000202 - subject: magnesium_ion predicate: inhibits object: pg_hydrolase_activity description: Exogenous Mg2+ inhibits autolysins/cell-wall hydrolases. evidence: - reference: DOI:10.1038/s41598-021-04294-5 notes: Exogenous Mg2+ inhibits autolysins/CW hydrolases and compensates increased DL-endopeptidase activity (clearest environmental modifier edge). predicate_id: RO:0002212 curation_history: - timestamp: '2026-05-05T01:35:46.921682+00:00' curator: seed_from_metpo action: SEEDED_FROM_METPO changes: imported from data/raw/metpo.owl (CLASS) llm_assisted: false - timestamp: '2026-05-11T22:30:00-07:00' curator: claude action: CURATED_CAUSAL_GRAPH changes: Added DOI-backed causal graph linking MreB-directed Rod-complex peptidoglycan synthesis to small cell width (0.5–0.65 μm). llm_assisted: true - timestamp: '2026-05-20T03:35:31Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 2 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (METPO:2000202×1, rdfs:subClassOf×1). llm_assisted: true - timestamp: '2026-05-26T05:00:48Z' curator: claude action: RENAME_PREDICATE_LABELS changes: 'Renamed 1 causal-edge predicate label(s) to align with existing groundings: organizes → enables ×1.' llm_assisted: true - timestamp: '2026-05-26T05:00:50Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 1 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002327×1). llm_assisted: true - timestamp: '2026-06-23T00:00:00Z' curator: claude action: ENRICH_CAUSAL_GRAPH changes: Added 6 evidence-backed generic edges (6 new nodes) from the deep-research report. llm_assisted: true - timestamp: '2026-06-24T17:21:13Z' curator: claude action: GROUND_CAUSAL_PREDICATES changes: Grounded 5 causal-edge predicate_id field(s) via mappings/predicate_grounding.tsv (RO:0002213×2, RO:0002326×1, METPO:2000202×1, RO:0002212×1). llm_assisted: true - timestamp: '2026-06-24T17:23:33Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 1 causal-node grounding field(s) via mappings/node_grounding.tsv (CHEBI:18420×1). llm_assisted: true - timestamp: '2026-06-26T07:15:19Z' curator: claude action: GROUND_CAUSAL_NODES changes: Grounded 1 causal-node grounding field(s) via mappings/node_grounding.tsv (UniProtKB:A0A1L9R356×1). llm_assisted: true