identifier: traitmech:000666 label: fungal peloton formation definition: A morphological phenotype in which a fungus forms intracellular coiled hyphal masses, called pelotons, in association with a plant host. definition_source: DOI:10.1111/nph.14279 trait_category: MORPHOLOGY term_kind: CLASS mapping_status: PROPOSED parent_traits: - METPO:1000059 evidence: - reference: DOI:10.1111/nph.14279 snippet: In the colonised protocorm cells, ORM fungi form coiled hyphae, known as pelotons notes: 'Fochi et al. (2017), author final manuscript, Discussion, Nitrogen transfer inside the mycorrhizal orchid protocorm, printed p. 15 / physical p. 17, line 465: https://iris.unito.it/retrieve/e27ce429-fbc3-2581-e053-d805fe0acbaa/N_metabolism_ORM_rev10_4aperto.pdf Contiguous clause matched after removing standalone line numbers and joining PDF line wraps, and visually checked. The maintained resolver returned NOT_IN_ABSTRACT; this is a manual full-text check, not a resolver VERIFIED result. Main text through Conclusions and actual Figure 5 were inspected. Figure 5 shows intact and collapsed pelotons in Serapias vomeracea 30 days after sowing with Tulasnella calospora. The manuscript''s title wording and GEO(XXXX) placeholder differ from final metadata; no dataset accession is inferred. Other figure images and supplementary contents were not inspected. Transcript patterns and heterologous yeast assays are not causal tests of native peloton formation.' - reference: DOI:10.1111/1365-2745.70188 snippet: Ericoid hyphal coils (also called pelotons) are specific structures formed by ericoid fungi in root epidermal cells notes: Meyers et al. (2025), Table 1 continued, CCI row, Relevance column, p. 3681 / physical p. 4. The complete morphology clause was exact-matched and visually checked in the public publisher PDF deposited at https://freidok.uni-freiburg.de/data/273892; its SHA256 matches the repository checksum. This directly read usage supports ericoid scope, not an orchid-only definition. Methods 2.4 scores Vaccinium myrtillus root intersects, distinguishing coils from other intracellular hyphae; CCI is not a fungal cell fraction or measured nutrient flux. No fungal species was identified for a canonical example. Actual supplementary Figure S3 was not inspected. The resolver returned UNRESOLVED; an exact Europe PMC DOI query returned no hit and Crossref confirmed the exact DOI/title. Manual PDF verification does not change that resolver verdict. - reference: DOI:10.1111/nph.19338 snippet: tightly coiled intracellular fungal hyphae (i.e. pelotons) are a constant feature of OrM notes: 'Perotto and Balestrini, Viewpoint, online 2023, institutional early-view PDF: https://iris.unito.it/retrieve/b44ea427-adb1-4f5b-a5b6-cc876e060f2f/New%20Phytologist%20-%202023%20-%20Perotto%20-%20At%20the%20core%20of%20the%20endomycorrhizal%20symbioses%20intracellular%20fungal%20structures%20in%20orchid.pdf Printed/physical p. 2, The peloton: a constant feature in orchid mycorrhiza. Main text and actual Figures 1-3 were read; the contiguous clause was visually checked. This is review terminology, not independent experimental replication. The same page describes Paris-type AM coils as resembling orchid pelotons and discusses a continuum with arbusculate coils; resemblance is not exact equivalence. Figure 2 has inconsistent panel/scale labels, so no panel-specific dimensions are adopted. Its hypothesis of plant-driven development is not an established formation mechanism. The orchid discussion does not erase the separate ericoid usage in Meyers et al.' canonical_examples: - taxon_id: NCBITaxon:156515 taxon_label: Tulasnella calospora reference: DOI:10.1111/nph.14279 note: AL13/4D = MUT4182 in Serapias vomeracea protocorms, oat medium, 20 C in darkness; actual Figure 5 documents pelotons 30 days after sowing. Fochi Methods, printed p. 4 / physical p. 6, https://iris.unito.it/retrieve/e27ce429-fbc3-2581-e053-d805fe0acbaa/N_metabolism_ORM_rev10_4aperto.pdf reports isolation from Anacamptis laxiflora mycorrhizal roots in northern Italy and deposition as MUT4182 (citing Girlanda et al. 2011, DOI:10.3732/ajb.1000486; that original isolation paper was not read). NCBI EFetch confirms species 156515 and child strain 1051891, Tulasnella calospora MUT 4182, with equivalent name Tulasnella calospora AL13/4D. The paper's engineered yeast complementation assay is a separate experiment, not an engineered fungal exemplar. This culture-qualified example is not independent taxonomic reidentification or evidence for every strain and condition. discussions: - discussion_id: fungal-peloton-scope-and-hierarchy prompt: Resolve a closer parent without collapsing intracellular architectures. kind: CURATION_TODO status: OPEN rationale: Use phenotype METPO:1000059. Spiral shaped METPO:1000684, branched shaped METPO:1000687 and filament shaped METPO:1000674 concern cell shape. Mycelial growth traitmech:000074 is explicitly bacterial. Fungal arbuscule formation traitmech:000664 concerns branched intracellular structures; hyphal anastomosis traitmech:000605 is fusion, pseudohyphal growth traitmech:000653 budding-cell chains, and haustorium formation traitmech:000663 a specialized host interface. None is an exact duplicate or settled closer parent. Fochi's orchid usage and Meyers' ericoid usage both inform the unqualified label. The review's Paris-type AM comparison does not make every intracellular coil a peloton or establish disjoint organisms. No exact synonyms, structure xrefs or SSSOM equivalences are asserted. Liverwort terminology in DOI:10.1098/rstb.2000.0617 remains an abstract-only analogy lead; its full text and figures were not inspected. posed_by: codex posed_date: '2026-10-07' - discussion_id: fungal-peloton-formation-versus-function prompt: Separate formation from nutrient transfer and proposed regulation. kind: KNOWLEDGE_GAP status: OPEN rationale: 'The phenotype does not require a measured nutrient flux, exclusive mutualism, a universal lifespan, or a specific plant-driven mechanism. Transcript associations and isolated transporter complementation do not establish the native formation pathway. Nutrient transfer before or during coil degeneration is a separate question from morphogenesis. A protein-resolved causal graph is deferred pending direct formation perturbations and authority-verified protein anchors; a mechanism is not claimed biologically absent. Keep the example''s MUT4182 separate from Tulasnella sp. SV6 (MUT4178) studied in DOI:10.1186/s43008-024-00165-6: that paper uses AL13/4D as a reference genome, not as its culture.' posed_by: codex posed_date: '2026-10-07' curation_history: - timestamp: '2026-10-07T15:40:03Z' curator: codex action: MINTED_TRAITMECH_ID changes: 'Added fungal peloton formation with two primary DOI-backed sources, a separately identified review, three snippets and a culture-qualified Tulasnella calospora example. Ignored-and-hidden novelty and main/worktree/open-PR reservation checks support 000666 and v542 block 1061900-1061999, with BAS #1788 reserved separately. Kept morphology distinct from nutrient flux and unproven formation mechanisms.' llm_assisted: true