identifier: METPO:1007084
label: catalase negative
definition: Test-outcome phenotype where the catalase test yields a negative result
  (no bubbling on H2O2). The underlying enzyme-organism relation should additionally
  be asserted via <organism> METPO:2000303 'does not show activity of' GO:0004096
  'catalase activity'.
trait_category: OTHER
term_kind: CLASS
mapping_status: SEEDED
parent_traits:
- METPO:1007080
synonyms:
- synonym_text: catalase -
  synonym_type: EXACT_SYNONYM
  source: metpo.owl
- synonym_text: catalase test negative
  synonym_type: EXACT_SYNONYM
  source: metpo.owl
created_by: Marcin Joachimiak
curation_history:
- timestamp: '2026-09-11T10:12:15Z'
  curator: seed_from_metpo
  action: SEEDED_FROM_METPO
  changes: imported from data/raw/metpo.owl (CLASS)
- timestamp: '2026-09-11T10:12:30Z'
  curator: codex
  action: ENRICHED_FROM_LITERATURE
  changes: Enriched the seeded METPO catalase negative test-outcome phenotype with
    DOI-backed assay and Enterococcus faecium evidence after a repository-wide duplicate
    review covering ignored and hidden files.
  llm_assisted: true
- timestamp: '2026-09-11T14:03:00Z'
  curator: codex
  action: RESOLVED_PARENT_GAP
  changes: Reparented catalase negative below the newly curated METPO:1007080 catalase
    test assay parent and marked the temporary assay-parent discussion resolved.
  llm_assisted: true
definition_source: DOI:10.1038/srep03081
evidence:
- reference: DOI:10.1038/srep03081
  snippet: The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized
    as foam
  notes: Iwase et al. tie the catalase assay signal to oxygen bubbles from hydrogen-peroxide
    decomposition, supporting no visible bubbling as a negative test outcome.
- reference: DOI:10.1128/iai.72.8.4512-4520.2004
  snippet: E. faecium is catalase negative, and the major mechanism to scavenge hydrogen
    peroxide is NADH peroxidase
  notes: Moy et al. identify E. faecium as catalase-negative and contrast its NADH-peroxidase
    scavenging with catalase-bearing E. faecalis under heme-supplemented conditions.
canonical_examples:
- taxon_id: NCBITaxon:1352
  taxon_label: Enterococcus faecium
  note: Moy et al. describe E. faecium as catalase-negative in the context of hydrogen-peroxide
    accumulation and scavenging.
  reference: DOI:10.1128/iai.72.8.4512-4520.2004
discussions:
- discussion_id: catalase-negative-assay-parent-gap
  prompt: Resolve a non-assay parent for negative catalase-test phenotypes before
    narrowing parent_traits below phenotype.
  kind: CURATION_TODO
  status: RESOLVED
  rationale: METPO:1007080 is now represented as the seeded catalase-test assay parent,
    so this negative assay-outcome phenotype can use its source superclass rather
    than a temporary direct phenotype parent.
  posed_by: codex
  posed_date: '2026-09-11'