identifier: METPO:1005010
label: indole test
definition: An assay that tests the ability of an organism to produce indole from
  tryptophan.
trait_category: OTHER
term_kind: CLASS
mapping_status: SEEDED
parent_traits:
- METPO:1000059
curation_history:
- timestamp: '2026-09-12T21:35:36Z'
  curator: seed_from_metpo
  action: SEEDED_FROM_METPO
  changes: imported from data/raw/metpo.owl (CLASS)
- timestamp: '2026-09-12T21:38:12Z'
  curator: codex
  action: ENRICHED_FROM_LITERATURE
  changes: Added the METPO indole-test assay parent with stable-URL tryptophan-to-indole
    assay evidence after a repository-wide duplicate review covering ignored and hidden
    files.
  llm_assisted: true
- timestamp: '2026-09-12T21:50:37Z'
  curator: codex
  action: CURATION_REVIEW_REVISION
  changes: 'Preserved the ASM source-local tryptophan citation marker after Claude
    Code Review issue #874 so the second evidence snippet is visibly a complete sentence.'
  llm_assisted: true
definition_source: https://asm.org/protocols/indole-test-protocol
evidence:
- reference: https://asm.org/protocols/indole-test-protocol
  snippet: The indole test screens for the ability of an organism to degrade the amino
    acid tryptophan and produce indole.
  notes: The ASM protocol defines the indole test as an assay for organism-level tryptophan
    degradation to indole.
- reference: https://asm.org/protocols/indole-test-protocol
  snippet: The chief requirement for culturing an organism prior to performing the
    indole test is that the medium contains a sufficient quantity of tryptophan (5).
  notes: The protocol explains that the indole-test medium needs tryptophan so production
    of indole can report tryptophan degradation.