identifier: METPO:1005011
label: indole test positive
definition: A phenotype in which an organism tests positive in the indole test, indicating
  it produces indole from tryptophan.
trait_category: OTHER
term_kind: CLASS
mapping_status: SEEDED
parent_traits:
- METPO:1005010
synonyms:
- synonym_text: indole test
  synonym_type: RELATED_SYNONYM
  source: metpo.owl
curation_history:
- timestamp: '2026-09-11T06:46:27Z'
  curator: seed_from_metpo
  action: SEEDED_FROM_METPO
  changes: imported from data/raw/metpo.owl (CLASS)
- timestamp: '2026-09-11T07:00:00Z'
  curator: codex
  action: ENRICHED_FROM_LITERATURE
  changes: Enriched the active METPO indole-test-positive phenotype with DOI-backed
    indole production evidence after a repository-wide duplicate review covering ignored
    and hidden files.
  llm_assisted: true
- timestamp: '2026-09-12T21:38:12Z'
  curator: codex
  action: RESOLVED_PARENT_GAP
  changes: Reparented indole test positive below the newly curated METPO:1005010 indole
    test assay parent and marked the temporary assay-parent discussion resolved.
  llm_assisted: true
definition_source: DOI:10.1128/AEM.02787-15
evidence:
- reference: DOI:10.1128/AEM.02787-15
  snippet: Indole, a bacterial product of tryptophan degradation
  notes: Darkoh et al. support microbial indole production as a tryptophan-degradation
    phenotype and evaluate indole detection in bacterial cultures.
- reference: DOI:10.1128/AEM.02787-15
  snippet: enterotoxigenic Escherichia coli strain H10407 produces 3.3 ± 0.22 mM indole
    during a 24-h period in the presence of 5 mM tryptophan
  notes: Darkoh et al. measured indole production by the enterotoxigenic E. coli H10407
    strain under tryptophan-supplemented culture conditions.
canonical_examples:
- taxon_id: NCBITaxon:316401
  taxon_label: Escherichia coli ETEC H10407
  note: Enterotoxigenic E. coli H10407 produced millimolar indole after 24 h of growth
    with tryptophan supplementation.
  reference: DOI:10.1128/AEM.02787-15
discussions:
- discussion_id: indole-test-positive-assay-parent-gap
  prompt: Resolve a non-assay parent for positive indole-test phenotypes before narrowing
    parent_traits below phenotype.
  kind: CURATION_TODO
  status: RESOLVED
  rationale: METPO:1005010 is the seeded superclass of METPO:1005011, but METPO:1005010
    defines an assay rather than a broader microbial trait class, so this record is
    temporarily parented directly to METPO:1000059 phenotype.
  posed_by: codex
  posed_date: '2026-09-11'
  resolved_date: '2026-09-12'
  resolution_note: METPO:1005010 is now represented as the seeded indole-test assay
    parent, so this assay-outcome phenotype can use its exact source superclass instead
    of a temporary direct phenotype parent; this resolves the exact METPO assay-parent
    gap, not the broader non-assay parent question for assay-outcome phenotypes.