identifier: METPO:1007086
label: oxidase negative
definition: Test-outcome phenotype where the oxidase test yields a negative result.
  The underlying enzyme-organism relation should additionally be asserted via <organism>
  METPO:2000303 'does not show activity of' GO:0004129 'cytochrome-c oxidase activity'.
trait_category: OTHER
term_kind: CLASS
mapping_status: SEEDED
parent_traits:
- METPO:1007081
synonyms:
- synonym_text: oxidase -
  synonym_type: EXACT_SYNONYM
  source: metpo.owl
- synonym_text: oxidase test negative
  synonym_type: EXACT_SYNONYM
  source: metpo.owl
created_by: Marcin Joachimiak
curation_history:
- timestamp: '2026-09-11T15:15:36Z'
  curator: seed_from_metpo
  action: SEEDED_FROM_METPO
  changes: imported from data/raw/metpo.owl (CLASS)
- timestamp: '2026-09-11T15:16:21Z'
  curator: codex
  action: ENRICHED_FROM_LITERATURE
  changes: Enriched the seeded METPO oxidase-negative test outcome with OMP definition
    provenance plus stable-URL and DOI-backed assay evidence after a repository-wide
    duplicate review covering ignored and hidden files.
  llm_assisted: true
definition_source: http://purl.obolibrary.org/obo/OMP_0006084
evidence:
- reference: https://asm.org/protocols/oxidase-test-protocol
  snippet: The test can be used to distinguish Neisseria gonorrhoeae (oxidase positive)
    from Staphylococcus spp. and Streptococcus spp. (oxidase negative)
  notes: The American Society for Microbiology protocol gives oxidase-negative Staphylococcus
    and Streptococcus spp. as contrasts to an oxidase-positive Neisseria gonorrhoeae
    result.
- reference: DOI:10.5812/chbs-160199
  snippet: A positive result from the dry filter paper method is indicated by the
    appearance of a dark purple color within 5 to 10 seconds. A 'delayed positive'
    result is recognized when a color change occurs between 10 and 60 seconds, whereas
    a negative result is indicated by the paper remaining colorless or changing color
    after more than 60 seconds
  notes: Hafezi and Khamar review the oxidase-test polarity and interpret a colorless
    dry filter paper, or a color change only after the cutoff, as a negative result.
- reference: DOI:10.5812/chbs-160199
  snippet: Bacteria that are classified as oxidase-negative can be anaerobic, aerobic,
    or facultative. A negative oxidase result indicates merely the absence of cytochrome
    c oxidase, which is responsible for oxidizing the reactant, while these organisms
    may utilize alternative oxidases for electron transfer during respiration
  notes: The review constrains interpretation of the negative assay outcome to cytochrome
    c oxidase under the oxidase-test readout, rather than absence of every terminal
    oxidase.