identifier: METPO:1005018
label: Voges-Proskauer test negative
definition: A phenotype in which an organism tests negative in the Voges-Proskauer
  test.
trait_category: OTHER
term_kind: CLASS
mapping_status: SEEDED
parent_traits:
- METPO:1005016
curation_history:
- timestamp: '2026-09-12T22:08:40Z'
  curator: seed_from_metpo
  action: SEEDED_FROM_METPO
  changes: imported from data/raw/metpo.owl (CLASS)
- timestamp: '2026-09-12T22:09:04Z'
  curator: codex
  action: ENRICHED_FROM_LITERATURE
  changes: Enriched the seeded METPO Voges-Proskauer-test-negative phenotype with
    stable-URL VP-negative interpretation evidence after a repository-wide duplicate
    review covering ignored and hidden files.
  llm_assisted: true
definition_source: https://asm.org/getmedia/40946f85-9357-4563-aa8a-994427efa825/Methyl-Red-and-Voges-Proskauer-Test-Protocols.pdf
evidence:
- reference: https://asm.org/getmedia/40946f85-9357-4563-aa8a-994427efa825/Methyl-Red-and-Voges-Proskauer-Test-Protocols.pdf
  snippet: VP-positive E. areogenes (A) shows red coloration on top of the culture,
    whereas VP-negative E. coli (B) has a yellowish color.
  notes: The ASM protocol figure caption identifies the yellow E. coli reaction after
    adding Barritt's reagents as the VP-negative comparison.
canonical_examples:
- taxon_id: NCBITaxon:562
  taxon_label: Escherichia coli
  note: ASM Figure 2 presents E. coli grown in MR-VP broth for 48 hours as the VP-negative
    control culture that remains yellowish after Barritt's reagents are added.
  reference: https://asm.org/getmedia/40946f85-9357-4563-aa8a-994427efa825/Methyl-Red-and-Voges-Proskauer-Test-Protocols.pdf