identifier: METPO:1005017
label: Voges-Proskauer test positive
definition: A phenotype in which an organism tests positive in the Voges-Proskauer
  test, indicating acetoin production.
trait_category: OTHER
term_kind: CLASS
mapping_status: SEEDED
parent_traits:
- METPO:1005016
synonyms:
- synonym_text: voges-proskauer test
  synonym_type: RELATED_SYNONYM
  source: metpo.owl
curation_history:
- timestamp: '2026-09-12T19:49:19Z'
  curator: seed_from_metpo
  action: SEEDED_FROM_METPO
  changes: imported from data/raw/metpo.owl (CLASS)
- timestamp: '2026-09-12T19:50:21Z'
  curator: codex
  action: ENRICHED_FROM_LITERATURE
  changes: Added the METPO Voges-Proskauer-test-positive phenotype with stable-URL
    and DOI-backed acetoin-production evidence after a repository-wide duplicate review
    covering ignored and hidden files.
  llm_assisted: true
- timestamp: '2026-09-12T21:15:44Z'
  curator: codex
  action: RESOLVED_PARENT_GAP
  changes: Reparented Voges-Proskauer test positive below the newly curated METPO:1005016
    Voges-Proskauer test assay parent and marked the temporary assay-parent discussion
    resolved.
  llm_assisted: true
definition_source: https://asm.org/getmedia/40946f85-9357-4563-aa8a-994427efa825/Methyl-Red-and-Voges-Proskauer-Test-Protocols.pdf
evidence:
- reference: https://asm.org/getmedia/40946f85-9357-4563-aa8a-994427efa825/Methyl-Red-and-Voges-Proskauer-Test-Protocols.pdf
  snippet: In the presence of KOH the intermediate acetoin is oxidized to diacetyl,
    a reaction which is catalyzed by a-naphthol (2). Diacetyl reacts with the guanidine
    group associated with molecules contributed by peptone in the medium, to form
    a pinkish-red-colored product (Fig. 2A).
  notes: The ASM protocol PDF explains that the Voges-Proskauer reaction detects acetoin
    through Barritt chemistry that forms a pinkish-red product.
- reference: DOI:10.1128/JCM.42.8.3665-3669.2004
  snippet: 'In the ASM Manual of Clinical Microbiology, only 6 of the 18 biochemical
    tests used in the present investigation are listed for Klebsiella species: indole,
    MR, VP, LDC, ODC, and malonate'
  notes: Hansen et al. evaluated Voges-Proskauer as a conventional Klebsiella biochemical
    test listed in the ASM Manual of Clinical Microbiology.
canonical_examples:
- taxon_id: NCBITaxon:571
  taxon_label: Klebsiella oxytoca
  note: Hansen et al. Table 2 reported 100.0% Voges-Proskauer-positive results for
    K. oxytoca across the CPHL, NRL, and SSI reference laboratories.
  reference: DOI:10.1128/JCM.42.8.3665-3669.2004
discussions:
- discussion_id: voges-proskauer-test-positive-assay-parent-gap
  prompt: Resolve a non-assay parent for positive Voges-Proskauer-test phenotypes
    before narrowing parent_traits below phenotype.
  kind: CURATION_TODO
  status: RESOLVED
  rationale: METPO:1005016 is the seeded superclass of METPO:1005017, but METPO:1005016
    defines an assay rather than a broader microbial trait class, so this record is
    temporarily parented directly to METPO:1000059 phenotype.
  posed_by: codex
  posed_date: '2026-09-12'
  resolved_date: '2026-09-12'
  resolution_note: METPO:1005016 is now represented as the seeded Voges-Proskauer-test
    assay parent, so this positive assay-outcome phenotype can use its exact source
    superclass instead of a temporary direct phenotype parent; this resolves the exact
    METPO assay-parent gap, not the broader non-assay parent question for assay-outcome
    phenotypes.