identifier: traitmech:000161
label: alanine arylamidase activity
definition: A physiological enzyme-activity phenotype in which a cell exhibits alanine
  arylamidase/alanyl aminopeptidase activity, releasing N-terminal residues from peptide,
  amide, or arylamide substrates with preference for alanine.
definition_source: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/2.html
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
synonyms:
- synonym_text: alanyl aminopeptidase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/2.html
- synonym_text: alanine aminopeptidase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/2.html
- synonym_text: L-alanine aminopeptidase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/2.html
evidence:
- reference: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/2.html
  snippet: <b>Reaction:</b> Release of an N-terminal amino acid, Xaa<img src="../../../EZgif/FISS.GIF"
    align=top>Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but
    may be most amino acids including Pro (slow action).
  notes: The NC-IUBMB EC 3.4.11.2 entry accepts membrane alanyl aminopeptidase and
    places the scissile-bond FISS.GIF image between Xaa and Yaa in a reaction line
    that includes arylamide substrates and preferential N-terminal alanine.
- reference: DOI:10.1099/ijsem.0.006017
  snippet: 'enzymatic activities are present for: α-galactosidase, N-acetyl-β-glucosaminidase,
    arginine arylamidase, proline arylamidase, leucyl glycine arylamidase, phenylalanine
    arylamidase, leucine arylamidase, tyrosine arylamidase, alanine arylamidase, glycine
    arylamidase, histidine arylamidase, glutamyl glutamic acid arylamidase, serine
    arylamidase, valine arylamidase, cysteine arylamidase, esterase, esterase lipase,
    alpha-chymotrypsin'
  notes: Srinivasan et al. listed alanine arylamidase among enzymatic activities present
    in the Amygdalobacter indicium species description, supporting the API arylamidase
    row as a bacterial enzyme-activity phenotype.
canonical_examples:
- taxon_id: NCBITaxon:3029272
  taxon_label: Amygdalobacter indicium
  note: Srinivasan et al. reported Amygdalobacter indicium as positive for alanine
    arylamidase activity in an enzyme activity panel.
  reference: DOI:10.1099/ijsem.0.006017
discussions:
- discussion_id: alanine-arylamidase-activity-xref-gap
  prompt: Resolve an exact external ontology class for alanine arylamidase activity
    before adding a TraitRecord xref.
  kind: CURATION_TODO
  status: OPEN
  rationale: GO:0016285 alanyl aminopeptidase activity denotes the catalytic molecular
    function rather than the organism-level alanine arylamidase/alanyl aminopeptidase
    phenotype, so it is appropriate as a causal-node grounding for that enzyme activity
    and not as an equivalent TraitRecord xref.
  posed_by: codex
  posed_date: '2026-09-12'
curation_history:
- timestamp: '2026-09-12T07:32:57Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted alanine arylamidase activity as a URL/DOI-backed TraitRecord after
    a repository-wide duplicate review covering ignored and hidden files; METPO has
    no exact alanine arylamidase activity class yet.
  llm_assisted: true