identifier: traitmech:000164
label: arginine dihydrolase activity
definition: A physiological pathway-activity phenotype in which a cell converts L-arginine
  through the arginine deiminase pathway to generate ATP.
definition_source: DOI:10.1128/aem.07724-11
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
synonyms:
- synonym_text: arginine deiminase pathway activity
  synonym_type: EXACT_SYNONYM
  source: DOI:10.1016/j.resmic.2012.11.004
- synonym_text: ADI pathway activity
  synonym_type: EXACT_SYNONYM
  source: DOI:10.1016/j.resmic.2012.11.004
- synonym_text: arginine dihydrolase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC3/5/3/6.html
- synonym_text: arginine deiminase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC3/5/3/6.html
evidence:
- reference: https://iubmb.qmul.ac.uk/enzyme/EC3/5/3/6.html
  snippet: <b>Reaction:</b> <small>L</small>-arginine + H<small><sub>2</sub></small>O
    = <small>L</small>-citrulline + NH<small><sub>3</sub></small>
  notes: The NC-IUBMB EC 3.5.3.6 entry grounds the first arginine-deiminase step of
    the arginine deiminase pathway and records arginine dihydrolase as another name
    for that molecular function.
- reference: DOI:10.1016/j.fm.2010.11.016
  snippet: Arginine conversion through the ADI pathway, which was activated from the
    stationary growth phase on, resulted in the production of both citrulline and
    ornithine for all pH conditions tested.
  notes: Rimaux et al. directly assayed pH-dependent L-arginine conversion through
    the ADI pathway in Lactobacillus sakei CTC 494.
- reference: DOI:10.1128/aem.07724-11
  snippet: For instance, the ability to utilize arginine through the arginine deiminase
    (ADI) pathway, resulting in additional ATP, represents a competitive benefit.
  notes: Rimaux et al. frame arginine utilization through the ADI pathway as an ATP-yielding
    metabolic capability and show that arc gene expression is strain- and pH-dependent
    in Lactobacillus sakei.
- reference: DOI:10.1016/j.resmic.2012.11.004
  snippet: Arginine conversion through the arginine deiminase (ADI) pathway is a common
    metabolic trait of Lactobacillus sakei which is ascribed to an arc operon and
    which inquisitively involves citrulline excretion and re-uptake.
  notes: Rimaux et al. use the pathway-activity phrasing while studying the transporter
    coupled to citrulline and ornithine exchange during L. sakei ADI pathway activity.
canonical_examples:
- taxon_id: NCBITaxon:1599
  taxon_label: Latilactobacillus sakei
  note: Rimaux et al. assayed arginine conversion through the ADI pathway in Lactobacillus
    sakei CTC 494 and observed citrulline and ornithine production across the tested
    pH series.
  reference: DOI:10.1016/j.fm.2010.11.016
discussions:
- discussion_id: arginine-dihydrolase-activity-xref-gap
  prompt: Resolve exact external ontology mappings for arginine dihydrolase activity
    before adding TraitRecord xrefs.
  kind: CURATION_TODO
  status: OPEN
  rationale: GO:0016990 arginine deiminase activity and EC 3.5.3.6 denote the first-step
    molecular function in the arginine deiminase pathway rather than the full organism-level
    pathway-activity phenotype, so GO:0016990 is a close mechanism grounding for the
    source node and not an equivalent TraitRecord xref.
  posed_by: codex
  posed_date: '2026-09-12'
curation_history:
- timestamp: '2026-09-12T09:44:56Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted arginine dihydrolase activity as a URL/DOI-backed TraitRecord after
    a repository-wide duplicate review covering ignored and hidden files; METPO has
    no exact arginine deiminase pathway activity class yet.
  llm_assisted: true