identifier: traitmech:000714 label: behavioral habituation definition: A physiological phenotype in which repeated stimulation produces an experience-dependent decrease in a microbial organism's behavioral response that is not attributable solely to sensory adaptation or motor fatigue. definition_source: DOI:10.1098/rspb.2016.0446 trait_category: PHYSIOLOGY term_kind: CLASS mapping_status: PROPOSED parent_traits: - METPO:1000059 evidence: - reference: DOI:10.1098/rspb.2016.0446 snippet: The Q and CAF slime moulds crossing a bridge containing quinine or caffeine for the sixth time showed reduced aversive behaviour. notes: 'Boisseau et al. 2016, PMID:27122563, PMC4855389; page 4, Results (b), Test phase 1. Q and CAF denote cultured Physarum polycephalum plasmodia repeatedly exposed to quinine or caffeine bridges. Five daily exposures precede same/alternate-repellent tests; two plain-agar days precede retesting. Contact latency, crossing time and shape are different readouts. Caffeine does not show every quinine effect: initial crossing time was not slower than control, and recovery was incomplete. Residual aversiveness remains. Alternate-repellent responses support stimulus specificity, not universal exclusion of every confound. Main Methods, Results, Discussion and all five figures were inspected in a public publisher-formatted PDF bearing a 2016 download stamp, not verified as the current corrected VoR. Supplementary statistics and raw data were not assessed. The contiguous quote crosses PDF columns; direct text and visual checks agree. Native abstract lookup: NOT_IN_ABSTRACT.' - reference: DOI:10.1016/j.cub.2025.05.071 snippet: the response to the low force stimuli shows extensive habituation even though the response to the alternating high force stimuli does not. notes: 'Rajan and Marshall 2025, PMID:40562036, PMC13499368; Figure 4F caption, concluding result clause in retained PMC author-manuscript HTML. Figure 4F is an experimental contraction response in cultured Stentor coeruleus, not the adjacent model panel. Preserved stronger-stimulus responses argue against general motor exhaustion. The caption describes identical control timing, while Methods describes one low-force control tap per minute versus alternating forces at one total tap per minute; exact cadence equivalence remains unresolved. Figure 4I uses intervening low-force stimulation, not a stimulus-free rest; Figure 5 separately tests recovery after training. Anchored healthy nondividing cells were selected from Ward''s cultures; field-of-view denominators vary. Experimental Results, relevant Methods and actual Figures 4-7 were inspected. Receptor internalization is modeled, not an identified molecular mechanism. Supplements and simulations were not reanalyzed. Native abstract lookup: NOT_IN_ABSTRACT; the exact full-text caption match is a separate check.' - reference: DOI:10.1016/j.cub.2026.03.080 snippet: For cells treated with either cycloheximide or puromycin, the primary effect on learning was accelerating habituation (Figure 1C, 1E). notes: 'Rajan et al. 2026, PMID:42025171, PMC13192338; Results S3, retained PMC author manuscript. Stentor contraction assays compare drug-treated and vehicle cultures under repeated mechanical stimulation. Figure 1C uses puromycin with two-hour pretreatment; Figure 1E describes 12-hour cycloheximide pretreatment, unlike general short-term Methods prose. Dose, stimulus strength and cadence differ; conditions are not interchangeable. Drug effects do not prove universally protein-synthesis-independent learning. Relevant Results and Methods and actual Figures 1, 3, 4 and 6 were inspected, not all omics analyses or supplements. RNAi and pharmacology retain off-target, anchoring, feeding and subculture limitations. SteCoe_6763 recovery differences were not significant. Figure 6 follows division during continuing stimuli, not retention after withdrawal. Results and Methods point to different supplementary electrical-control panels. This follow-up shares a laboratory with the 2025 study, not an independent replication. Native abstract lookup: NOT_IN_ABSTRACT; directly matched Results quote.' - reference: DOI:10.1016/j.nlm.2008.09.012 snippet: Habituation is defined as a behavioral response decrement that results from repeated stimulation and that does not involve sensory adaptation/sensory fatigue or motor fatigue. notes: Rankin et al. 2009, PMID:18854219, PMC2754195; section 2, page 136 (PDF page 2), complete opening definition. This consensus review supports operational terminology, not a microbial experiment or independent replication. Its nervous-system framing is not imposed on non-neural microbes. The public institutional PDF was directly read and pages 2-4 visually inspected. PDF ligatures are expanded and whitespace folded for matching; no paraphrase is substituted. Partial recovery is allowed. Responding to a novel stimulus is not itself dishabituation, which restores the original response. The behavioral criteria are not a demonstrated common molecular mechanism. Source-qualified MED identity was checked; its absent commentCorrectionList is not exhaustive notice clearance. discussions: - discussion_id: habituation-scope-and-hierarchy prompt: Retain a behavioral endpoint and distinguish neighboring uses of adaptation. kind: CURATION_TODO status: OPEN rationale: 'Repeated-stimulus decrement alone is insufficient: evaluate sensory adaptation, motor fatigue, injury/toxicity, selection and depletion alternatives in the actual assay. Specificity and recovery provide operational support without identifying all underlying mechanisms. Rankin 2009 frames habituation in neural behavioral terms; Rajan 2025 distinguishes that usage from cellular adaptation while modeling related receptor dynamics. This record concerns microbial behavior, not a categorical ban on shared molecular machinery. No fixed time, curve, complete recovery, dishabituation or all ten hallmarks are required in every experiment. Response to a stronger stimulus is not itself dishabituation. Carbon-source memory (traitmech:000706), acquired stress-resistance memory (traitmech:000712), enhanced transcriptional reactivation memory (traitmech:000713), anticipation, bet hedging and nutrient adaptation have distinct endpoints. Chemotaxis is not a parent of every behavioral assay. METPO:1000059 phenotype is the conservative parent; generic learning, memory and adaptation are not asserted as exact synonyms.' posed_by: codex posed_date: '2026-10-13' - discussion_id: habituation-evidence-and-mechanism-limits prompt: Resolve source discrepancies and culture provenance before adding taxa or mechanisms. kind: KNOWLEDGE_GAP status: OPEN rationale: Different taxa and assays support the phenotype, not independent replication of each experiment. Laboratory culture provenance has not been independently resolved for natural canonical taxa; no species-wide assignment follows. Unread supplements, raw data and statistical reanalysis remain gaps. The 2025 control cadence, 2026 cycloheximide timing and electrical-control locators remain unresolved source discrepancies. Retained public PDFs and author manuscripts are not verified equivalent to current corrected publisher versions. Bounded MED/Crossref checks are not proof of notice absence. Modeled receptor internalization, exploratory omics and drug/RNAi effects do not justify a universal protein-resolved mechanism. No causal graph, protein examples, canonical taxa or exact external mappings are asserted. Access failures, source hashes and actual snippet-verifier results are in the sidecar. posed_by: codex posed_date: '2026-10-13' - discussion_id: habituation-historical-identity prompt: Reconcile deprecated adaptation neighbors without reviving their identifiers. kind: CURATION_TODO status: OPEN rationale: Pinned and inspected current METPO graphs mark METPO:1000004 adaptation, METPO:1000005 adaptive trait, METPO:0000248 and METPO:000249 Microbial Adaptation deprecated below ObsoleteClass, with OMO:0001000 and no inspected replacement or consider annotations. METPO issue 521 disputes the mechanically assigned reasons. Original historical scopes remain unresolved; labels do not prove equivalence or nonequivalence. This operational behavioral phenotype is accepted independently from primary evidence, not by reviving an obsolete term or inferring biological invalidity. Available ignored/hidden files, retained tracked history, pending changes, ontology graphs and live corpus semantics revealed no exact active record in the inspected scope. No historical exact mapping is asserted. The pinned ontology and existing records remain unchanged; discovery is not exhausted. posed_by: codex posed_date: '2026-10-13' curation_history: - timestamp: '2026-10-13T04:41:26Z' curator: codex action: MINTED_TRAITMECH_ID changes: Added behavioral habituation with three primary DOI sources and one terminology review, each with a contiguous snippet. Qualified assay, version, mechanism and historical-identity limitations. Reserved 000714, v590 and 1066700-1066799 after scoped ignored/hidden, retained-history, independent-copy and pending-PR checks, with numeric false positives explicitly adjudicated. Existing records unchanged; no canonical taxa or causal graph. Timestamp is the observed UTC post-audit confirmation, not an estimated write time. llm_assisted: true