identifier: traitmech:000151
label: beta-glucuronidase activity
definition: A physiological enzyme-activity phenotype in which a cell produces active
  beta-glucuronidase enzymes that hydrolyze beta-D-glucuronosides to D-glucuronate
  and an alcohol.
definition_source: https://iubmb.qmul.ac.uk/enzyme/EC3/2/1/31.html
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
evidence:
- reference: https://iubmb.qmul.ac.uk/enzyme/EC3/2/1/31.html
  snippet: 'Reaction: a β-D-glucuronoside + H2O = D-glucuronate + an alcohol'
  notes: The NC-IUBMB EC 3.2.1.31 entry defines beta-glucuronidase by its accepted
    name and hydrolytic reaction, grounding the enzyme activity named by the organism-level
    phenotype.
- reference: DOI:10.1128/aem.50.6.1383-1387.1985
  snippet: The beta-glucuronidase produced by E. coli cleaves the MUG substrate to
    yield a fluorescent end product.
  notes: Moberg used beta-glucuronidase cleavage of MUG to detect Escherichia coli
    in food samples.
canonical_examples:
- taxon_id: NCBITaxon:562
  taxon_label: Escherichia coli
  note: Moberg reported beta-glucuronidase-mediated cleavage of MUG by Escherichia
    coli in a fluorogenic detection assay.
  reference: DOI:10.1128/aem.50.6.1383-1387.1985
discussions:
- discussion_id: beta-glucuronidase-activity-xref-gap
  prompt: Resolve an exact external ontology class for beta-glucuronidase activity
    before adding a TraitRecord xref.
  kind: CURATION_TODO
  status: OPEN
  rationale: GO:0004566 carries the same beta-glucuronidase activity label but denotes
    the enzyme molecular function rather than the organism-level beta-glucuronidase
    production phenotype, so it is appropriate as a causal-node grounding rather than
    an equivalent TraitRecord xref.
  posed_by: codex
  posed_date: '2026-09-12'
curation_history:
- timestamp: '2026-09-12T00:37:27Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted beta-glucuronidase activity as a URL/DOI-backed TraitRecord after
    a repository-wide duplicate review covering ignored and hidden files; METPO has
    no exact beta-glucuronidase activity class yet.
  llm_assisted: true
- timestamp: '2026-09-12T00:55:15Z'
  curator: codex
  action: FIX_EVIDENCE_SNIPPET
  changes: Corrected the IUBMB EC 3.2.1.31 water token from HTML-derived H_{2}O to
    source-visible H2O after Claude Code Review flagged the snippet as non-verbatim
    in https://github.com/CultureBotAI/TraitMech/issues/807.
  llm_assisted: true