identifier: traitmech:000710 label: functional cellular rejuvenation definition: A physiological phenotype in which age-associated loss of cellular performance or replicative potential is reversed in a microbial cell or reset in its descendants. definition_source: DOI:10.1242/jcs.86.1.263 trait_category: PHYSIOLOGY term_kind: CLASS mapping_status: PROPOSED parent_traits: - METPO:1000059 evidence: - reference: DOI:10.1242/jcs.86.1.263 snippet: This is the first description of the restoration of a senescent defect by microinjection of young cytoplasmic components into Paramecium. notes: Haga and Karino 1986, PMID:3654779; scientific abstract, directly read in the MED record. Young-cell cytoplasm restored sexual activity in senescent cells (about 700 fissions); recovery persisted for up to 20 fissions. Division rate and fertility after self-conjugation did not improve. This supports endpoint-specific recovery in treated old cells, not whole-cell or permanent rejuvenation. Full methods and figures were unavailable. - reference: DOI:10.1038/s41467-018-06154-9 snippet: As this convergence was now downwards, doubling times decreased with each generation. notes: 'Proenca et al. 2018, PMID:30213942, PMC6137065; Results, Stable equilibria are connected by aging and rejuvenation (Sec6), directly checked in primary XML. Actual Figure 4d was visually inspected: new-daughter lineages from the old-cell equilibrium converge toward faster growth over successive generations. These doubling times are derived from elongation rates, not direct interdivision intervals. Recovery is in descendants, not the same old mother. The tested MG1655 conditions do not establish all-environment recovery or a universal damage mechanism.' - reference: DOI:10.1126/science.1204349 snippet: Gametes (spores) generated by aged cells show the same replicative potential as gametes generated by young cells. notes: Unal et al. 2011, PMID:21700873, PMC3923466; scientific abstract, directly checked in MED and an MIT-hosted author manuscript. Main text and Figure 1 support gamete replicative-potential reset. Figure 4 concerns the separate vegetative lifespan-extension experiment. Transient NDT80-dependent vegetative lifespan extension is a separate result and does not establish reversal of every aged-cell defect. Supplements and strain tables were not assessed. The retrieved PDF identifies the article but differs in size and checksum from MIT bitstream metadata; original deposited-byte identity remains unresolved. - reference: DOI:10.3390/microorganisms11010082 snippet: The cloned immaturin gene induces sexual rejuvenation in both mature and senescent cells by microinjection. notes: 'Haga 2023, PMID:36677375, PMC9865852; scientific abstract, directly checked in primary XML and PMC text. Results 3.1 uses rejuvenation for suppression of mating in mature cells: that maturation-state reset alone is outside this functional trait. Results 3.2 reports recovery of old-cell mating activity after transformation. Figure 6C measures total cell counts over four days, not direct division times, and lacks a young-cell growth comparator. Engineered treatment is not a natural canonical taxon example; inferred chemistry is not a proven mechanism.' - reference: DOI:10.1016/j.isci.2022.103765 snippet: Some cells rejuvenated immediately, even after extended starvation, but others only restarted growth after a delay or not at all. notes: 'Cesar et al. 2022, PMID:35243217, PMC8858994; scientific abstract, directly checked against MED and primary XML. Boundary evidence: culture duration during stationary phase is not individual replicative age. Immediate regrowth can reflect preserved function, not recovery from a demonstrated deficit. Regrowth classes use area-derived growth rates; nongrowth during the observation window is not proof of irreversible death. Results 2.1 and 2.3, Discussion, limitations and classification methods were read; figures and raw data were not independently assessed.' discussions: - discussion_id: cellular-rejuvenation-operational-scope prompt: Assess the functional qualifier and distinguish same-cell recovery from descendant reset. kind: CURATION_TODO status: OPEN rationale: Require an age-linked deficit and a measured recovery or replicative-potential reset, retaining the endpoint, age axis, comparison group, treated unit, conditions and persistence. Recovery of one function need not restore all functions, be permanent or extend lifespan. Descendant recovery must not be reported as repair of the old mother. Marker changes, lifespan extension alone, prevention without prior loss and sexual immaturity alone are insufficient. Bare cellular rejuvenation has broader source usages and is not an exact synonym. Autogamy, gametogenesis and dormancy exit are not themselves this phenotype; neither is replicative aging with functional decline. Culture age is not interchangeable with lineage age. METPO:1000059 phenotype is a conservative parent, with no reparenting of existing records or asserted external equivalence. posed_by: codex posed_date: '2026-10-12' - discussion_id: cellular-rejuvenation-mechanism-and-example-limits prompt: Resolve strain provenance and endpoint-specific mechanisms before adding examples or causal edges. kind: KNOWLEDGE_GAP status: OPEN rationale: This is a reusable physiological outcome, not a gene, protein, sequence feature or intervention. No canonical example or protein-resolved graph is asserted pending strain provenance and mechanistic review. Bacterial model-derived damage and fluorescent aggregates are not direct proof of a universal rejuvenation chemistry. Studies need not share a mechanism. The 2011 PDF/metadata byte mismatch is retained, not explained away by its added cover. MED/Crossref notice fields and accessible primary pages were checked on 2026-10-12; incomplete publisher access prevents an exhaustive notice-absence claim. Ignored/hidden local and pending-source searches plus pinned and current released METPO found no exact term in the inspected scope. No ontology upgrade or discovery-exhaustion claim follows. posed_by: codex posed_date: '2026-10-12' curation_history: - timestamp: '2026-10-12T12:13:34Z' curator: codex action: MINTED_TRAITMECH_ID changes: Added functional cellular rejuvenation with five primary DOI references and directly checked snippets; retained same-cell, descendant, maturation and dormancy boundaries and access limits. Reserved 000710, v586 and 1066300-1066399 after ignored/hidden, registered historical-tree and pending-PR checks. Examples and mechanism graph deferred. Existing records unchanged; timestamp is the observed UTC curation decision. llm_assisted: true - timestamp: '2026-10-12T12:40:17Z' curator: codex action: CORRECTED_FIGURE_ATTRIBUTION changes: 'Corrected issue #1893: Figure 1 supports gamete replicative-potential reset; Figure 4 tests vegetative lifespan extension. Preserved definition, DOI, snippet, status, checksum limitation and original event. The source sidecar retains its historical assessment with an explicit superseding correction. Timestamp is the observed UTC correction decision.' llm_assisted: true