identifier: traitmech:000147
label: cystine arylamidase activity
definition: A physiological enzyme-activity phenotype in which a cell produces active
  cystine arylamidase enzymes that hydrolyze cystine arylamide substrates.
definition_source: DOI:10.14202/vetworld.2024.143-149
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
evidence:
- reference: DOI:10.14202/vetworld.2024.143-149
  snippet: Cystine arylamidase 7.5 L-cystyl-2-naphthylamide
  notes: Ludfiani et al. list L-cystyl-2-naphthylamide as the API ZYM cystine arylamidase
    substrate, grounding the organism-level phenotype as an arylamide-hydrolyzing
    enzyme activity.
- reference: DOI:10.3389/fmicb.2022.1034816
  snippet: Positive alkaline phosphatase, cystine arylamidase, esterase(C4), esterase
    lipase(C8), leucine arylamidase, naphthol-AS-B1-phosphohydrolase, valine arylamidase
  notes: Jiang et al. detected cystine arylamidase in the Geminicoccus harenae type
    strain with the API ZYM enzyme activity panel.
- reference: DOI:10.2323/jgam.60.59
  snippet: In API ZYM, activities are positive for alkaline phosphatase, esterase
    (C4), leucine arylamidase, valine arylamidase, cystine arylamidase
  notes: An et al. reported cystine arylamidase among positive API ZYM activities
    for the Flavobacterium panaciterrae DCY69T type strain.
canonical_examples:
- taxon_id: NCBITaxon:2498453
  taxon_label: Geminicoccus harenae
  note: Jiang et al. reported Geminicoccus harenae as positive for cystine arylamidase
    in the API ZYM enzyme activity panel.
  reference: DOI:10.3389/fmicb.2022.1034816
discussions:
- discussion_id: cystine-arylamidase-activity-xref-gap
  prompt: Resolve an exact external ontology class for cystine arylamidase activity
    before adding a TraitRecord xref.
  kind: CURATION_TODO
  status: OPEN
  rationale: GO:0004177 covers aminopeptidase activity at molecular-function scope
    and no live GO class provides an exact residue-specific cystine arylamidase production
    phenotype at organism-level scope.
  posed_by: codex
  posed_date: '2026-09-11'
curation_history:
- timestamp: '2026-09-11T19:19:57Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted cystine arylamidase activity as a DOI-backed TraitRecord after a
    repository-wide duplicate review covering ignored and hidden files; METPO has
    no exact cystine arylamidase activity class yet.
  llm_assisted: true