identifier: traitmech:000693 label: diatom perizonium production definition: A biomineralization phenotype in which a diatom produces the siliceous band system called the perizonium in an auxospore wall. definition_source: DOI:10.1371/journal.pone.0272778 trait_category: PHYSIOLOGY term_kind: CLASS mapping_status: PROPOSED parent_traits: - traitmech:000690 evidence: - reference: DOI:10.1371/journal.pone.0272778 snippet: The deposition of the primary TP band became evident when the auxospore center attained a slim and rigid appearance. notes: 'Kaczmarska et al. 2022, PMID:36067191, PMC9447881, Results: Epizonium and perizonium. TP means transverse perizonium. Scientific Abstract, Introduction, Methods, early Results, this subsection and Discussion: Auxospore structure and development read in official Europe PMC XML on 2026-10-10; actual Figure 11 inspected. Other actual figures and S1 Appendix unread. EXT_ID:36067191 AND SRC:MED confirmed ID, title, DOI and scientific abstract. Clone HK328 (= ECT3902) originated from Florida Bay material in 2011; atypical morphology and prolonged cultivation limit canonical assignment. PDMPO labels silica deposited after addition. SEM resolves inner TP/LP bands apart from initial valves; fixed images do not establish live kinetics. Authors leave some epizonium/perizonium layer boundaries uncertain. Scaly-band architecture and proposed shape control are not universal or demonstrated necessities.' - reference: DOI:10.1371/journal.pone.0181413 snippet: transverse perizonial bands produced all together or in quick succession rather than being added to the auxospore apex one at a time notes: 'Kaczmarska et al. 2017, PMID:28813426, PMC5558960, scientific Abstract; EXT_ID:28813426 AND SRC:MED confirmed identity. Abstract, Methods prose, Results: Auxospore wall fine structure, related Discussion paragraphs and actual Figure 5 read on 2026-10-10. Table 1/2 headers, footnotes and StA:7/8 and Van5:5/6 rows inspected; other rows not fully interpreted. Other actual figures and supplements unread. Fixed stages support the authors'' inferred collapsible-cup sequence, not a live secretion time course. No longitudinal perizonia found under their structural criterion; initial epivalves must be distinguished. Discussion retains the Tabularia fasciculata study (reference 100); reinterpretation targets other papers. Aged small cultures, original isolates and progeny are distinct; culture abnormalities do not define canonical morphology.' - reference: DOI:10.1016/j.ejop.2013.06.002 snippet: Silica precipitation began throughout the auxospore at or near maximal length, but initially was detectable in isolated regions throughout the structure. notes: Mather et al., online 2013-08-22, 2014 issue, PMID:23972513. Complete scientific Abstract directly read in official Europe PMC CORE on 2026-10-10 using EXT_ID:23972513 AND SRC:MED; one exact MED result with matching ID, title and DOI. Tabularia fasciculata under the source name lacked transverse perizonium; longitudinal deposition preceded initial valves near maximal auxospore length. Undetectable silicon by EDS is not absolute absence. Publisher full text unavailable; Methods, actual figures and supplements unread. These three primary studies have overlapping authors, not independent-laboratory replication. discussions: - discussion_id: diatom-perizonium-production-scope-and-mappings prompt: Resolve exact mappings without conflating auxospore wall components. kind: CURATION_TODO status: OPEN rationale: Biomineralization traitmech:000690 is strictly broader. This is production, not a material band, inherited band possession, silica uptake, or auxosporulation alone. Incunabular scales, epizonium, initial valve/girdle frustules and setae are not automatically this endpoint. Scaly bands in mediophytes do not justify universal parenting to siliceous scale production. Existing frustule/seta boundary notes are not unresolved exact perizonium groundings. No requirement for both transverse and longitudinal bands, a single geometry, one-at-a-time secretion, deposition during expansion, or sexual origin is imposed. No organism-level disjointness or fitness effect is asserted. Exact synonyms, properizonium terminology and external phenotype equivalences remain unasserted pending authority review; structure terms are not automatically phenotype xrefs. posed_by: codex posed_date: '2026-10-10' - discussion_id: diatom-perizonium-production-exemplars-and-mechanisms prompt: Resolve canonical study material and formation-specific causal evidence. kind: KNOWLEDGE_GAP status: OPEN rationale: 'No canonical examples are assigned: source clone origins were read, but current taxonomy and figure-to-mating-pair attribution need reconciliation, including atypical or aged cultures. Keep mineral deposition, band patterning and shape control separate. Static microscopy, PDMPO/EDS observations and sequence features alone do not establish a conserved causal protein mechanism or necessity for shape control. Protein edges require functional evidence and taxon-paired accessions. Mechanism is deferred, not claimed absent.' posed_by: codex posed_date: '2026-10-10' curation_history: - timestamp: '2026-10-10T23:15:32Z' curator: codex action: MINTED_TRAITMECH_ID changes: Added diatom perizonium production beneath biomineralization with three primary DOI citations and directly checked short snippets. Ignored-and-hidden main/worktree/open-PR reservation checks support 000693 and v569 block 1064600-1064699. Existing trait records unchanged; mappings, canonical taxa and mechanisms remain open. Timestamp records the observed UTC curation decision. llm_assisted: true