identifier: traitmech:000731 label: eukaryotic flagellar autotomy definition: A physiological phenotype in which a microbial eukaryote sheds its cilia or flagella by regulated severing of their axonemes near the base. definition_source: DOI:10.1186/1471-2121-4-11 trait_category: PHYSIOLOGY term_kind: CLASS mapping_status: PROPOSED parent_traits: - METPO:1000059 evidence: - reference: DOI:10.1186/1471-2121-4-11 snippet: fla10 cells lose flagella by deflagellation at 33 degrees C. notes: Parker and Quarmby 2003, PMID:12930563, PMCID:PMC194436, scientific abstract Results, second sentence. Direct MED text match; native VERIFIED applies to that text, not the PDF degree-symbol typography. Background distinguishes basal axonemal severing from resorption. The quoted response concerns source-named Chlamydomonas fla10 in calcium-containing medium, not reference strain WTB214. Released flagella were counted per 100 cells, with the initial count subtracted; lengths are a separate endpoint. Low calcium is not calcium-free, and buffer exchange is a confound. All twelve main PDF pages and eight figures were inspected. No universal IFT or calcium requirement follows. - reference: DOI:10.1534/g3.116.034264 snippet: Wild-type cells deflagellate completely in response to pH shock or dibucaine, regardless of the temperature notes: Hilton et al. 2016, PMID:27520959, PMCID:PMC5068960.1, page 3416, Results and Discussion subsection ADF2 and ADF4 remain unassigned, first paragraph and Figure 4A. Contiguous JATS and typeset match; native NOT_IN_ABSTRACT retained. The comparison is 21 versus 33 degrees C after six hours, not every temperature. The assay scores the control-normalized decrease in biflagellate cells per 100 cells, not a direct count of released flagella or proof that every responding cell loses both. Figure 4A reports three independent experiments with SD. All ten main pages, four figures and three tables were inspected; supplements remain unread. Phototaxis screening alone is not the shedding endpoint. discussions: - discussion_id: eukaryotic-flagellar-autotomy-scope prompt: Review the basal axonemal shedding endpoint and its phenotype parent. kind: CURATION_TODO status: OPEN rationale: This organism-level phenotype concerns regulated release of a eukaryotic cilium or flagellum, not possession, arrangement, assembly failure or nonmotility. Resorption, retained-organelle disassembly, passive breakage, distal vesicle release and premitotic basal-body release alone do not establish the endpoint. Bacterial flagellar ejection or resorption involves a different apparatus and is outside this qualified class. The phenotype parent METPO:1000059 is a definition-based inference; motility is not a required parent. Palmelloid formation traitmech:000654 does not require flagellar loss. No exact synonyms, organism-level disjointness or universal stimulus threshold are asserted. posed_by: codex posed_date: '2026-10-15' - discussion_id: eukaryotic-flagellar-autotomy-metpo prompt: Review current ontology scope without reviving obsolete morphology identifiers. kind: CURATION_TODO status: OPEN rationale: The inspected pinned and current METPO release/editor/template scope has no active exact shedding class. Historical METPO:0000089 and METPO:000090 are deprecated with OMO:0001000; METPO:1000381 is deprecated with IAO:0000226. All point to METPO:1000704, which denotes locomotory flagellar possession, not autotomy. Deprecated ciliated METPO:0000093 and METPO:000094 carry OMO:0001000, while METPO:1000385 carries IAO:0000226; no replacement or consider annotation was observed for these or deprecated flagellum METPO:1000123, whose recorded reason is OMO:0001000. Those historical morphology terms are not exact xrefs and are not reactivated. METPO issue https://github.com/berkeleybop/metpo/issues/521 records unresolved reason-code interpretation. Current upstream describes a bacterial/archaeal focus, so adoption of this microbial-eukaryote proposal requires maintainer review. The ontology pin stays unchanged. Proposal v607 reserves METPO:1068400 within 1068400-1068499; no upstream release or human sign-off is implied. posed_by: codex posed_date: '2026-10-15' - discussion_id: eukaryotic-flagellar-autotomy-mechanism prompt: Keep the shedding phenotype separate from universal katanin or sequence-based mechanism claims. kind: KNOWLEDGE_GAP status: OPEN rationale: A protein-resolved graph requires taxon-paired authority-checked accessions and edge-specific experiments. Domain, motif, gene or mutant names alone do not establish the organismal phenotype. The p60 study constrains a proposed universal severing mechanism; it does not establish dispensability of every katanin function. No universal FA1, FA2, TRP15, IFT or calcium requirement is asserted. Mechanisms are deferred, not claimed absent. evidence: - reference: DOI:10.1242/jcs.096941 reference_title: PF19 encodes the p60 catalytic subunit of katanin and is required for assembly of the flagellar central apparatus in Chlamydomonas supports: SUPPORT evidence_source: full_text snippet: microtubule severing activity of p60 is not required for deflagellation explanation: Supports the bounded mechanistic limitation, not an additional definition citation or a claim that every katanin function is dispensable. notes: Dymek and Smith 2012, PMID:22467860, PMCID:PMC3516377, Discussion subsection Is katanin required for flagellar excision in Chlamydomonas? Direct HTML match; native NOT_IN_ABSTRACT retained. Main text and all six figures were inspected; supplements were not. Figure 5A assays cellular shedding; Figure 2 assays purified protein. Transcript knockdown is not verified protein depletion. The reference A54-e18 carries listed mutations, and the tested mutants are not nulls. posed_by: codex posed_date: '2026-10-15' - discussion_id: eukaryotic-flagellar-autotomy-provenance prompt: Resolve strain provenance and preserve source-access limits before adding canonical taxa. kind: KNOWLEDGE_GAP status: OPEN rationale: Study organism and strain names remain qualified observations, not canonical taxon assertions. Distinct papers from the Quarmby group are not independent-laboratory replication. MED/Crossref fields and available PubMed/publisher records were checked for notices; this is not comprehensive correction/retraction clearance. Publisher DOI requests failed in the follow-up, some PMC requests returned challenge pages, and the 2003 PubMed page yielded no usable text. Those failures do not negate earlier directly retrieved primary materials, but are not successful source checks. The 2016 supplementary index digest mismatch remains recorded; nine substantive assets matched supplied digests. No paid research, human sign-off or raw-data reanalysis is implied. posed_by: codex posed_date: '2026-10-15' curation_history: - timestamp: '2026-10-15T07:50:51Z' curator: codex action: MINTED_TRAITMECH_ID changes: Added the microbial eukaryotic basal-axoneme shedding phenotype with two primary DOI sources and directly checked 10/15-word snippets. Retained native VERIFIED and NOT_IN_ABSTRACT outcomes respectively, plus separate 10-word full-text mechanism-limitation evidence with native NOT_IN_ABSTRACT. Reserved 000731, v607 and 1068400-1068499 after ignored/hidden current-file, retained-history, full-range and open/draft PR checks. No obsolete identifier, canonical taxon, exact mapping or universal protein requirement is inferred. The timestamp records observed acceptance, not a reconstructed acquisition time. llm_assisted: true