identifier: traitmech:000178
label: leucyl glycine arylamidase activity
definition: A physiological enzyme-activity phenotype in which a cell produces active
  leucyl-glycine arylamidase enzymes that hydrolyze leucyl-glycine arylamide substrates.
definition_source: DOI:10.1099/ijsem.0.006017
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
synonyms:
- synonym_text: leucyl glycine arylamidase
  synonym_type: RELATED_SYNONYM
  source: DOI:10.1099/ijsem.0.006017
- synonym_text: leucylglycine arylamidase
  synonym_type: RELATED_SYNONYM
  source: DOI:10.1128/JCM.40.9.3427-3431.2002
evidence:
- reference: DOI:10.1099/ijsem.0.006017
  snippet: 'enzymatic activities are present for: α-galactosidase, N-acetyl-β-glucosaminidase,
    arginine arylamidase, proline arylamidase, leucyl glycine arylamidase, phenylalanine
    arylamidase, leucine arylamidase, tyrosine arylamidase, alanine arylamidase, glycine
    arylamidase, histidine arylamidase, glutamyl glutamic acid arylamidase, serine
    arylamidase, valine arylamidase, cysteine arylamidase, esterase, esterase lipase,
    alpha-chymotrypsin'
  notes: Srinivasan et al. listed leucyl glycine arylamidase among enzymatic activities
    present in the Amygdalobacter indicium species description, supporting the API
    arylamidase row as a directly assayed bacterial enzyme-activity phenotype.
- reference: DOI:10.1128/JCM.40.9.3427-3431.2002
  snippet: When the API rapid ID32A system was used, all eight strains displayed activity
    for alanine arylamidase, arginine arylamidase, α-glucosidase, glycine arylamidase,
    histidine arylamidase, leucine arylamidase, leucylglycine arylamidase, proline
    arylamidase, phenylalanine arylamidase, serine arylamidase, and tyrosine arylamidase
  notes: Hall et al. reported leucylglycine arylamidase activity in all eight Actinomyces
    cardiffensis clinical isolates tested with the API rapid ID32A system.
canonical_examples:
- taxon_id: NCBITaxon:3029272
  taxon_label: Amygdalobacter indicium
  note: Srinivasan et al. reported Amygdalobacter indicium as positive for leucyl
    glycine arylamidase activity in an enzyme activity panel.
  reference: DOI:10.1099/ijsem.0.006017
discussions:
- discussion_id: leucyl-glycine-arylamidase-activity-xref-gap
  prompt: Resolve an exact external ontology class for leucyl glycine arylamidase
    activity before adding a TraitRecord xref.
  kind: CURATION_TODO
  status: OPEN
  rationale: GO:0016805 covers broad dipeptidase activity at molecular-function scope,
    and no live GO class provides an exact leucyl-glycine-specific arylamidase production
    phenotype at organism-level scope.
  posed_by: codex
  posed_date: '2026-09-14'
curation_history:
- timestamp: '2026-09-14T00:53:00Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted leucyl glycine arylamidase activity as a DOI-backed TraitRecord
    after a repository-wide duplicate review covering ignored and hidden files; METPO
    has no exact leucyl glycine arylamidase activity class yet and the placeholder
    is reserved in proposals/metpo_traitmech_v55.
  llm_assisted: true
- timestamp: '2026-09-14T01:12:00Z'
  curator: codex
  action: UPDATED_DEFINITION
  changes: Corrected the PR 882 review wording issue tracked in issue 883 by defining
    the trait around leucyl-glycine arylamide substrates rather than free leucyl-glycine
    dipeptide substrates.
  llm_assisted: true