identifier: traitmech:000139 label: lipase activity definition: A physiological enzyme-activity phenotype in which a cell produces active lipases that hydrolyze triglycerides at lipid-water interfaces. definition_source: DOI:10.22207/JPAM.16.4.27 trait_category: PHYSIOLOGY term_kind: CLASS mapping_status: PROPOSED parent_traits: - METPO:1000059 synonyms: - synonym_text: lipase production synonym_type: RELATED_SYNONYM source: DOI:10.22207/JPAM.16.4.27 - synonym_text: lipolytic activity synonym_type: RELATED_SYNONYM source: DOI:10.1016/j.mex.2018.01.004 evidence: - reference: DOI:10.22207/JPAM.16.4.27 snippet: Lipase-producing bacteria were isolated using tributyrin agar as a selective medium notes: Savalia and Dungrechiya used tributyrin agar to isolate lipase-producing bacteria from waste samples. - reference: DOI:10.1016/j.mex.2018.01.004 snippet: to identify lipase producers. The addition of calcium and magnesium ions can provide an easier screening procedure for selection of lipolytic bacterial strains notes: Carrazco-Palafox et al. optimized modified tributyrin agar as a microbial lipolytic-activity screen. canonical_examples: - taxon_id: NCBITaxon:1408 taxon_label: Bacillus pumilus note: Bacillus pumilus S113 was isolated as the strongest lipase producer in a tributyrin-agar screen. reference: DOI:10.22207/JPAM.16.4.27 discussions: - discussion_id: lipase-activity-xref-gap prompt: Resolve an exact external ontology class for lipase activity before adding a TraitRecord xref. kind: CURATION_TODO status: OPEN rationale: GO:0016298 denotes the lipase molecular function rather than the organism-level lipase production phenotype, so it is too scope-shifted for an equivalent TraitRecord xref. posed_by: codex posed_date: '2026-09-11' curation_history: - timestamp: '2026-09-11T00:00:00Z' curator: codex action: MINTED_TRAITMECH_ID changes: Minted lipase activity as a DOI-backed TraitRecord after a repository-wide duplicate review covering ignored and hidden files; METPO has no exact lipase activity class yet. llm_assisted: true