identifier: traitmech:000165
label: NAD-dependent alcohol dehydrogenase activity
definition: A physiological enzyme-activity phenotype in which a cell produces active
  NAD-dependent alcohol dehydrogenases that interconvert primary or secondary alcohols
  with aldehydes or ketones.
definition_source: https://iubmb.qmul.ac.uk/enzyme/EC1/1/1/1.html
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
synonyms:
- synonym_text: alcohol dehydrogenase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC1/1/1/1.html
- synonym_text: ethanol dehydrogenase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC1/1/1/1.html
- synonym_text: NAD-dependent alcohol dehydrogenase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC1/1/1/1.html
evidence:
- reference: https://iubmb.qmul.ac.uk/enzyme/EC1/1/1/1.html
  snippet: '<b>Reaction:</b> (1) a primary alcohol + NAD<small><sup>+</sup></small>
    = an aldehyde + NADH + H<small><sup>+</sup></small><br>


    (2) a secondary alcohol + NAD<small><sup>+</sup></small> = a ketone + NADH + H<small><sup>+</sup></small>'
  notes: The NC-IUBMB EC 1.1.1.1 entry names alcohol dehydrogenase as alcohol:NAD+
    oxidoreductase and defines the NAD-dependent primary-alcohol-to-aldehyde and secondary-alcohol-to-ketone
    reactions.
- reference: DOI:10.1111/j.1432-1033.1986.tb09366.x
  snippet: The two alcohol dehydrogenases found in Zymomonas mobilis have each been
    purified using dye-ligand chromatography and affinity elution with nucleotides.
  notes: Neale et al. purified the ZADH-1 and ZADH-2 isoenzymes from Zymomonas mobilis
    and characterized their ethanol-oxidizing and acetaldehyde-reducing activities.
- reference: DOI:10.1128/jb.169.6.2591-2597.1987
  snippet: The gene which encodes alcohol dehydrogenase II (adhB) from Zymomonas mobilis
    was cloned in Escherichia coli
  notes: Conway et al. cloned the Z. mobilis adhB gene, connected it to the purified
    alcohol dehydrogenase II protein, and used an indicator plate that detected recombinant
    ADH activity.
canonical_examples:
- taxon_id: NCBITaxon:542
  taxon_label: Zymomonas mobilis
  note: Neale et al. purified and characterized the two native alcohol dehydrogenase
    isoenzymes from Zymomonas mobilis.
  reference: DOI:10.1111/j.1432-1033.1986.tb09366.x
discussions:
- discussion_id: nad-dependent-alcohol-dehydrogenase-activity-xref-gap
  prompt: Resolve an exact external ontology class for NAD-dependent alcohol dehydrogenase
    activity before adding a TraitRecord xref.
  kind: CURATION_TODO
  status: OPEN
  rationale: GO:0004022 and EC 1.1.1.1 denote the NAD-dependent molecular function
    rather than the organism-level NAD-dependent alcohol dehydrogenase production
    phenotype; the GO term remains an appropriate causal-node grounding lead but not
    an equivalent TraitRecord xref.
  posed_by: codex
  posed_date: '2026-09-12'
curation_history:
- timestamp: '2026-09-12T10:11:31Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted alcohol dehydrogenase activity as a URL/DOI-backed TraitRecord after
    a repository-wide duplicate review covering ignored and hidden files; METPO has
    no exact alcohol dehydrogenase activity class yet.
  llm_assisted: true
- timestamp: '2026-09-12T10:38:00Z'
  curator: codex
  action: REVIEW_FIX
  changes: 'Narrowed the label and definition to the NAD-dependent EC 1.1.1.1 phenotype
    after PR #833 review found the cofactor-agnostic alcohol dehydrogenase activity
    label overbroad for the evidence.'
  llm_assisted: true