identifier: traitmech:000174
label: naphthol-AS-BI-phosphohydrolase activity
definition: A physiological enzyme-activity phenotype in which a cell produces active
  phosphohydrolases that hydrolyze naphthol-AS-BI-phosphate substrates.
definition_source: DOI:10.14202/vetworld.2024.143-149
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
synonyms:
- synonym_text: naphthol-AS-BI-phosphohydrolase
  synonym_type: RELATED_SYNONYM
  source: DOI:10.14202/vetworld.2024.143-149
evidence:
- reference: DOI:10.14202/vetworld.2024.143-149
  snippet: Naphthol-AS-BI-phosphohydrolase 5.4 Naphthol-AS-BI-phosphate
  notes: Ludfiani et al. list Naphthol-AS-BI-phosphate as the API ZYM naphthol-AS-BI-phosphohydrolase
    substrate, grounding the organism-level phenotype as hydrolysis of the naphthol-AS-BI
    phosphate chromogenic substrate.
- reference: DOI:10.1128/JCM.43.8.3713-3717.2005
  snippet: Alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase,
    acid phosphatase, and naphthol-AS-BI-phosphohydrolase were clearly positive
  notes: Otsuka et al. detected clear naphthol-AS-BI-phosphohydrolase API ZYM reactions
    in the five Corynebacterium resistens isolates they characterized.
canonical_examples:
- taxon_id: NCBITaxon:258224
  taxon_label: Corynebacterium resistens
  note: Otsuka et al. detected clear naphthol-AS-BI-phosphohydrolase API ZYM reactions
    in Corynebacterium resistens isolates.
  reference: DOI:10.1128/JCM.43.8.3713-3717.2005
discussions:
- discussion_id: naphthol-as-bi-phosphohydrolase-activity-xref-gap
  prompt: Resolve an exact external ontology class for naphthol-AS-BI-phosphohydrolase
    activity before adding a TraitRecord xref.
  kind: CURATION_TODO
  status: OPEN
  rationale: GO:0016791 covers broad phosphatase activity at molecular-function scope,
    and no live GO class provides an exact naphthol-AS-BI-phosphate-specific phosphohydrolase
    production phenotype at organism-level scope.
  posed_by: codex
  posed_date: '2026-09-12'
curation_history:
- timestamp: '2026-09-12T15:09:52Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted naphthol-AS-BI-phosphohydrolase activity as a DOI-backed TraitRecord
    after a repository-wide duplicate review covering ignored and hidden files; METPO
    has no exact naphthol-AS-BI-phosphohydrolase activity class yet and the placeholder
    is reserved in proposals/metpo_traitmech_v51.
  llm_assisted: true