identifier: traitmech:000723
label: nematode egg-hatching inhibitory activity
definition: A physiological capability of a microorganism to reduce nematode egg hatching
  over a defined observation interval relative to comparable unexposed controls.
definition_source: DOI:10.1163/156854104323072883
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
evidence:
- reference: DOI:10.1163/156854104323072883
  snippet: The least hatches were recorded in 7-day-old C. globosum (L250) filtrate,
    with 85 and 98% hatch reductions for SCN and RKN, respectively.
  notes: 'Meyer et al. 2004, Results, printed page 28/PDF page 6; all ten pages and
    actual Figure 1/Table 1 inspected. C. globosum is Chaetomium globosum; SCN and
    RKN are Heterodera glycines and Meloidogyne incognita. Native UNRESOLVED; Crossref
    and USDA verify identity. Typeset span matches; PDF extraction loses the fi ligature.
    Seven days is culture age, not egg exposure: hatch was scored after 14 days, including
    motile and immotile juveniles. Percent hatch uses initial eggs, then uninoculated
    PDB hatch as comparator; quoted reductions are relative to that control, not egg
    mortality. Biomass-free filtrate concentrations differ between hosts (75/90%);
    approximately 200 eggs per well, five wells per treatment, water viability control.
    Forty isolates were retested, not all 253. pH was measured, not neutralized; no
    identified active molecule or universal potency threshold is inferred.'
- reference: DOI:10.1038/s41598-023-33669-z
  snippet: T. asperellum FbMi6 inhibited egg hatching in all concentrations as compared
    to control.
  notes: Saharan et al. 2023, PMID:37088805, Results; direct publisher HTML match,
    native NOT_IN_ABSTRACT. This is its own experiment, not its background account
    of Meyer. Five surface-sterilized egg masses received spore-checked culture filtrates
    at 20/40/60/80%, with water and PDB controls; alternate-day counts through ten
    days, two repeats at 25 +/- 2 degrees C. Actual Figure 3 inspected. Results associates
    maximum inhibition with 120 h, whereas its legend specifies 2/4/6/8/10 days; no
    exact maximum/time pairing is adopted. Egg deformation does not prove death. Juvenile
    water-recovery findings are not transferred to eggs.
discussions:
- discussion_id: nematode-egg-hatching-inhibition-scope-and-hierarchy
  prompt: Refine the hierarchy while preserving the microbial actor and hatching endpoint.
  kind: CURATION_TODO
  status: OPEN
  rationale: The microorganism has the inhibitory capability; this is not its own
    development or the nematode's susceptibility. Reduced hatching over an observed
    interval does not establish egg death, irreversible arrest or subsequent recovery.
    Juvenile paralysis or killing, fewer root egg masses, population suppression,
    trapping, attraction and sequence annotations alone are insufficient. Feeding
    and infection are not required. Related lethal, paralyzing, trapping, attracting
    and fungal-nematophagy records are not necessary parents; no organism-level disjointness
    is asserted. Phenotype METPO:1000059 is the conservative parent. Pinned/current
    METPO screening found only the unrelated fried-egg-shaped colony, not an exact
    active or deprecated counterpart in inspected owners; v599 proposes the class.
    Ovicidal terminology and nematicide chemical roles are not exact synonyms or xrefs.
    Neither a universal host/stage range nor a particular secretion mechanism is implied.
  posed_by: codex
  posed_date: '2026-10-14'
- discussion_id: nematode-egg-hatching-inhibition-source-and-mechanism-limits
  prompt: Resolve strain provenance and assay discrepancies before adding taxa or
    mechanisms.
  kind: KNOWLEDGE_GAP
  status: OPEN
  rationale: Meyer's USDA technical abstract reports six motility-inhibitory isolates,
    versus four in the published paper; that separate endpoint is not silently reconciled.
    Saharan's claimed FbMi6 accession resolves to clone SF_570, while its supplement
    has SF_561/SF_570 and FbMi-4 inconsistencies documented in the lethal-nematicidal
    source assessment. Do not infer a corrected strain or canonical taxon. No shared
    active chemical, microbial protein, causal graph, field efficacy or environmental
    safety is asserted. Culture-filtrate effects do not define the entire class as
    secretion. Source hashes, direct quote checks and bounded notice checks are retained
    in the verification sidecar. Meyer has no Crossmark data available; no comprehensive
    notice clearance or full raw-data/statistical reanalysis is claimed.
  posed_by: codex
  posed_date: '2026-10-14'
curation_history:
- timestamp: '2026-10-14T03:09:57Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Added microbial nematode egg-hatching inhibitory activity with two DOI-backed
    primary studies and directly checked 22-word and 13-word result excerpts. Retained
    assay denominators, exposure intervals, source discrepancies and native UNRESOLVED/NOT_IN_ABSTRACT
    outcomes. Distinguished hatch reduction from egg death, paralysis and reduced
    reproduction. Reserved 000723, v599 and 1067600-1067699 after ignored/hidden current-copy,
    retained-history and open/draft PR checks; broad editor ranges are not competing
    reservations. No exact active METPO counterpart identified in inspected owners.
    Other records unchanged. Timestamp records observed acceptance, not the later
    file write.
  llm_assisted: true