identifier: traitmech:000724
label: nematode egg-hatching stimulatory activity
definition: A physiological capability of a microorganism to increase nematode egg
  hatching over a defined observation interval relative to comparable controls without
  that microbial influence.
definition_source: DOI:10.1163/156854104323072883
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
evidence:
- reference: DOI:10.1163/156854104323072883
  snippet: Fifteen isolates (5.9%) produced compounds that strongly stimulated SCN
    egg hatch compared to PDB controls (Table 1).
  notes: Meyer et al. 2004, Results, printed page 28/PDF page 6, lower left. SCN denotes
    Heterodera glycines; PDB is potato dextrose broth. Native UNRESOLVED; Crossref
    and the USDA-hosted article verify identity. The typeset span was visually checked;
    PDFKit splits controls across lines, so plain whitespace normalization does not
    match. Hatch counts include motile and immotile juveniles at 14 days, normalized
    to initial eggs and then uninoculated PDB hatch. Three or seven days denotes culture
    age, not egg exposure. The source-specific strong-effect threshold combines effect
    size and statistical significance; it is not a universal trait cutoff. Forty isolates
    were retested, not all 253. Nutrient depletion versus added stimulants remains
    unresolved despite the quote's compound wording. pH was measured, not neutralized.
    Host, isolate and culture age can change effect direction.
- reference: DOI:10.1186/s13071-015-0986-z
  snippet: When incubated with Gram-positive bacteria, only T. muris eggs showed noticeable
    hatching after 20 h, although with high variability.
  notes: Vejzagic et al. 2015, PMID:26174801, PMCID:PMC4501204, scientific Abstract
    Results; exact normalized publisher/XML match and native VERIFIED. This is its
    own bacterial experiment, not a citation of Meyer. Full Results and actual Figure
    2 show Enterococcus caccae and Lactobacillus reuteri above bacteria-free controls
    after 20 h without antimicrobials, with substantial within- and between-run variability.
    Hatch is released larvae divided by initial embryonated eggs. T. muris and T.
    suis are Trichuris muris and Trichuris suis; little T. suis hatch in these conditions
    is not absolute inability. Antimicrobials and egg storage history affect interpretation.
    The E. coli strain/concentration ANOVA excludes near-zero controls; that analysis
    is not a statistical contrast against no bacteria. No direct-contact mechanism
    is inferred from background citations.
discussions:
- discussion_id: nematode-egg-hatching-stimulation-scope-and-hierarchy
  prompt: Refine the hierarchy without changing the microbial actor or positive-hatch
    endpoint.
  kind: CURATION_TODO
  status: OPEN
  rationale: 'This is a microbial capability to increase hatching, not merely lack
    of inhibition, the microorganism''s own development or the nematode''s susceptibility.
    Embryogenesis without emergence, attraction, trapping, juvenile paralysis or killing
    alone is insufficient. Feeding, infection and benefit to the microbe are not required.
    No organism-level disjointness from hatch inhibition is asserted: host and culture
    conditions can reverse responses. Phenotype METPO:1000059 is the conservative
    parent. Pinned/current METPO screening found only unrelated fried-egg-shaped colony,
    not an exact active or deprecated counterpart in inspected owners; v600 proposes
    the class. No exact synonym or xref is asserted. Neither host-generalism nor a
    secretion/contact mechanism is implied.'
  posed_by: codex
  posed_date: '2026-10-14'
- discussion_id: nematode-egg-hatching-stimulation-source-and-mechanism-limits
  prompt: Resolve host range, strain provenance and mechanism before adding canonical
    examples or causal edges.
  kind: KNOWLEDGE_GAP
  status: OPEN
  rationale: Meyer explicitly leaves nutrient depletion versus added compounds unresolved.
    Some fungal filtrates stimulate one host while inhibiting another, and culture
    age can reverse activity. The bacterial study reports variable hatch and contrasts
    two Trichuris hosts; neither study defines universal potency or host range. No
    canonical taxon, shared active molecule, protein or causal graph is inferred.
    No field efficacy, environmental safety, infectivity benefit or therapeutic use
    is asserted. Methods, own results and selected figures were inspected, not the
    full experimental raw data. Direct snippets and bounded notice checks are retained
    in the verification sidecar. Crossmark responses were script shells, not current-status
    verdicts; Meyer publisher access returned 403. No comprehensive correction/retraction
    clearance is claimed.
  posed_by: codex
  posed_date: '2026-10-14'
curation_history:
- timestamp: '2026-10-14T06:04:12Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Added microbial nematode egg-hatching stimulatory activity with two DOI-backed
    primary studies and directly checked 17-word and 19-word excerpts. Preserved native
    UNRESOLVED/VERIFIED outcomes, source-specific comparators, host dependence and
    mechanism uncertainty. Positive hatch enhancement is not merely lack of inhibition.
    Reserved 000724, v600 and 1067700-1067799 after ignored/hidden current-copy, retained-history
    and open/draft PR checks; decimal measurements, embedding coordinates and broad
    editor ranges are not competing reservations. No exact active METPO counterpart
    identified in inspected owners. Other records unchanged. Timestamp records observed
    acceptance, not later writing.
  llm_assisted: true