identifier: traitmech:000166
label: prolyl aminopeptidase activity
definition: A physiological enzyme-activity phenotype in which a cell produces active
  prolyl aminopeptidases that release N-terminal proline residues from peptides.
definition_source: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/5.html
trait_category: PHYSIOLOGY
term_kind: CLASS
mapping_status: PROPOSED
parent_traits:
- METPO:1000059
synonyms:
- synonym_text: proline aminopeptidase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/5.html
- synonym_text: Pro-X aminopeptidase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/5.html
- synonym_text: proline iminopeptidase
  synonym_type: RELATED_SYNONYM
  source: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/5.html
- synonym_text: proline arylamidase
  synonym_type: RELATED_SYNONYM
  source: DOI:10.1099/ijsem.0.006017
evidence:
- reference: https://iubmb.qmul.ac.uk/enzyme/EC3/4/11/5.html
  snippet: <b>Reaction:</b> Release of N-terminal proline from a peptide
  notes: The NC-IUBMB EC 3.4.11.5 entry accepts prolyl aminopeptidase, gives the N-terminal-proline
    release reaction, and records proline aminopeptidase, Pro-X aminopeptidase, and
    proline iminopeptidase as other names.
- reference: DOI:10.1111/j.1365-2958.1993.tb01249.x
  snippet: Proline iminopeptidase (Pip) is a hydrolase elaborated by virtually all
    strains of Neisseria gonorrhoeae that selectively removes N-terminal proline residues
    from peptides.
  notes: Albertson and Koomey cloned the gonococcal pip gene and confirmed that the
    encoded enzyme can release biologically active proline from peptides.
- reference: DOI:10.1099/ijsem.0.006017
  snippet: 'enzymatic activities are present for: α-galactosidase, N-acetyl-β-glucosaminidase,
    arginine arylamidase, proline arylamidase, leucyl glycine arylamidase, phenylalanine
    arylamidase, leucine arylamidase, tyrosine arylamidase, alanine arylamidase, glycine
    arylamidase, histidine arylamidase, glutamyl glutamic acid arylamidase, serine
    arylamidase, valine arylamidase, cysteine arylamidase, esterase, esterase lipase,
    alpha-chymotrypsin'
  notes: Srinivasan et al. listed proline arylamidase among enzymatic activities present
    in the Amygdalobacter indicium species description, supporting the API proline
    arylamidase row as a directly assayed bacterial enzyme-activity phenotype.
canonical_examples:
- taxon_id: NCBITaxon:485
  taxon_label: Neisseria gonorrhoeae
  note: Albertson and Koomey cloned the Neisseria gonorrhoeae proline iminopeptidase
    gene and confirmed that the encoded Pip enzyme releases proline from peptides.
  reference: DOI:10.1111/j.1365-2958.1993.tb01249.x
discussions:
- discussion_id: prolyl-aminopeptidase-activity-xref-gap
  prompt: Resolve an exact external ontology class for prolyl aminopeptidase activity
    before adding a TraitRecord xref.
  kind: CURATION_TODO
  status: OPEN
  rationale: GO:0004177 denotes broad aminopeptidase molecular function and EC 3.4.11.5
    denotes a prolyl aminopeptidase molecular function; both are scope-shifted relative
    to the organism-level prolyl aminopeptidase production phenotype, so they remain
    causal-node grounding leads rather than equivalent TraitRecord xrefs.
  posed_by: codex
  posed_date: '2026-09-12'
curation_history:
- timestamp: '2026-09-12T11:07:00Z'
  curator: codex
  action: MINTED_TRAITMECH_ID
  changes: Minted prolyl aminopeptidase activity as a URL/DOI-backed TraitRecord after
    a repository-wide duplicate review covering ignored and hidden files; METPO has
    no exact prolyl aminopeptidase activity class yet.
  llm_assisted: true