identifier: traitmech:000711 label: unequal protein aggregate inheritance at cell division definition: A physiological phenotype in which microbial cell division recurrently yields sibling cells with unequal numbers or amounts of inherited protein aggregates. definition_source: DOI:10.1371/journal.pbio.1001886 trait_category: PHYSIOLOGY term_kind: CLASS mapping_status: PROPOSED parent_traits: - METPO:1000059 evidence: - reference: DOI:10.1073/pnas.0708931105 snippet: their position in the mother cell determines which offspring inherits the inclusion body and where it will be located within it notes: Lindner et al. 2008, PMID:18287048, PMC2268587; Results, Inclusion Bodies Are Formed in Discrete Cellular Positions and Accumulate in Old Poles Under Nonstressing Conditions. Primary PMC Results and Methods directly read. The quoted position is that of an IbpA-YFP focus. MGAY is an engineered MG1655 reporter strain, grown on LB-agarose at 37 C without imposed stress. Figure 2 is the inspected localization measurement; Figure 3 is an inspected lineage schematic, not another experiment. Pole identity changes across divisions. These observations describe reporter-focus inheritance and eventual older-pole accumulation. Govers et al. 2018 (DOI:10.1371/journal.pbio.2003853) subsequently demonstrated label-induced clustering with IbpA-YFP. The older readout is not independent proof of endogenous unstressed aggregation; naming the engineered strain alone does not resolve this assay bias. Neither active transport, universal aggregate toxicity nor a natural canonical exemplar is established. Supplements were not inspected. The full-text span retains the native abstract resolver verdict NOT_IN_ABSTRACT. - reference: DOI:10.1371/journal.pbio.1001886 snippet: We experimentally confirmed that fusion increases segregation asymmetry, for a range of stresses, and identified Hsp16 as a fusion factor. notes: 'Coelho et al. 2014, PMID:24936793, PMC4061010; scientific abstract, not Author Summary. Primary XML and actual Figures 3, 4 and S4 were inspected. Hsp104-GFP fission-yeast imaging supports stress-associated unequal inheritance without consistent old/new-pole bias. Figure 3C measures an absolute sibling difference in aggregate-associated fluorescence, not concentration; S4I measures count asymmetry. Figure 4B is fusion and 4C is nucleation: the caption reverses them. A Results pointer to 4C for stress-dependent asymmetry disagrees with the plotted endpoints. Figure 3C describes continuous 40 C, whereas Methods describes 40 C for one hour followed by 37 C; this remains unresolved. Text S1 experimental methods and strain table were text-extracted, not visually rendered. No universal transport, death threshold or rejuvenation mechanism is inferred. The scientific-abstract span matches the native resolver text (VERIFIED).' - reference: DOI:10.1111/mmi.14228 snippet: Noticeably, stalked cells always inherited more aggregates (75%) than swarmer cells (25%). notes: Schramm et al. 2019, PMID:30779464, PMC6850343; Results, The aggregate load does not sort to swarmer or stalked daughter cells in C. crescentus. Primary XML Results, Discussion and relevant Methods were read; actual Figure 6 was inspected. DnaK-mVenus, delta-clpB cells recovered at 30 C after 10 minutes at 40 C. Figure 6G reports aggregate allocation across daughter cell types over successive divisions, not the fraction of all sibling pairs with unequal counts. Figure 6F measures projected cell area, not volume. Unequal shared inheritance is compatible with the broad phenotype; it contradicts neither sharing nor a stable allocation ratio. The paper limits universal exclusive old-pole retention, not every form of quantitative inequality. Supplements and independent strain provenance were not inspected; no natural canonical example is asserted. The full-text span retains the native abstract resolver verdict NOT_IN_ABSTRACT. - reference: DOI:10.1371/journal.pbio.2003853 snippet: the polarly deposited PA was subjected to limited disaggregation and therefore became asymmetrically inherited for a large number of generations notes: Govers et al. 2018, PMID:30153247, PMC6112618; scientific abstract, directly matched in primary XML and source-qualified MED. Results on reporter validation and post-stress inheritance, relevant Methods and actual Figures 1 and 3 were inspected. Figure 1 shows reporter-dependent clustering; Figure 3 separately follows engineered MG1655 IbpA-msfGFP lineages after 47 C for 15 minutes, with microscopy at 37 C. Focus inheritance is distinct from the fluorescence concentration and growth readouts. This does not validate unstressed IbpA-YFP foci as endogenous aggregates or imply universal harm, benefit, aging or rejuvenation. Supplementary controls were read only where described in Results; their images, raw data and independent strain provenance were not assessed. discussions: - discussion_id: unequal-aggregate-inheritance-operational-scope prompt: Assess the quantity-qualified definition and the level of inheritance evidence. kind: CURATION_TODO status: OPEN rationale: Require repeated lineage or population observations under stated conditions, not a single unequal sibling pair. Retain aggregate count versus fluorescence-derived amount, the time of measurement, reporter, strain and detection limits. Amount is not concentration; cell area is not volume. Within-pair inequality, cell-type allocation bias and complete aggregate exclusion are distinct readouts. Sharing between daughters need not be equal, and unequal inheritance need not favor a fixed pole or cell type. No universal threshold or programmed asymmetry is specified. Bare asymmetric segregation has narrower source usages and is not asserted as an exact synonym. Aggregate presence, aggrephagy, heat-shock response, morphological division asymmetry, replicative aging and functional rejuvenation are not equivalent traits or demonstrated narrower parents. Use METPO:1000059 phenotype without reparenting existing records. posed_by: codex posed_date: '2026-10-12' - discussion_id: unequal-aggregate-inheritance-mechanism-and-source-limits prompt: Resolve assay discrepancies, strain provenance and mechanisms before extending this first-pass record. kind: KNOWLEDGE_GAP status: OPEN rationale: 'This phenotype does not imply active transport, toxic damage, functional decline or rejuvenation. An aggregate-free descendant alone establishes none of those mechanisms or outcomes. The engineered reporter and deletion contexts remain qualified evidence, not natural canonical examples. No shared protein mechanism, graph or external equivalence is asserted. Coelho Figure 4 panel-label and Figure 3C versus Methods temperature discrepancies remain open; actual plotted endpoints are distinguished from text pointers. Primary source/metadata notice checks on 2026-10-12 were bounded by access; they do not prove exhaustive notice absence. Reporter-focus inheritance must be separated from endogenous aggregation: the later Govers study identifies IbpA-YFP label-induced clustering, while its separate post-heat-shock IbpA-msfGFP lineages support inheritance. Monomeric labels also require validation of expression level and assay conditions; no reporter is declared universally artifact-free.' posed_by: codex posed_date: '2026-10-12' - discussion_id: unequal-aggregate-inheritance-historical-metpo-match prompt: Retain the deprecated aggregate lexical hit without asserting identity or reusing its ID. kind: CURATION_TODO status: OPEN rationale: Pinned and current released METPO contain METPO:1000011, obsolete aggregate, marked deprecated and below ObsoleteClass. It has an IAO:0000231 annotation to OMO:0001000, but no definition, replacement or consider annotation in the inspected releases. Its original scope and reason-code interpretation remain unresolved. A lexical aggregate hit does not establish equivalence to unequal protein aggregate inheritance; this literature-defined phenotype was independently accepted. Do not reactivate 1000011 or include it as an exact xref. Ignored/hidden available-file and registered historical-tree searches, pending PRs and released RDF found no exact trait in the inspected scope; this is not discovery exhaustion. posed_by: codex posed_date: '2026-10-12' curation_history: - timestamp: '2026-10-12T14:38:12Z' curator: codex action: MINTED_TRAITMECH_ID changes: Added unequal protein aggregate inheritance at cell division with three primary DOI references and checked snippets. Retained quantity, population, assay and historical-METPO scope limits. Reserved 000711, v587 and 1066400-1066499 after ignored/hidden, registered historical-tree and pending-PR checks, distinguishing an editor namespace envelope from narrower reservations. Examples and mechanism graph deferred. Existing records unchanged; timestamp is the observed UTC curation decision. llm_assisted: true - timestamp: '2026-10-12T18:24:02Z' curator: codex action: QUALIFIED_REPORTER_EVIDENCE changes: 'Addressed issue #1896 by qualifying the Lindner reporter-focus readout against later primary evidence of IbpA-YFP label-induced clustering. Added Govers 2018 with a checked abstract snippet and separate post-stress inheritance context. Preserved identity, definition, parent, PROPOSED status, original event and source assessment; appended the correction and updated the proposal sources. Timestamp is the observed UTC correction decision.' llm_assisted: true