METADATA last updated: 2025-12-14 RT after BA fixed inconsistencies file_name: SOP-101-A_2 1X Inactivation Saline Solution Prep - Table Form.md file_date: 2022-08-24 title: FloodLAMP QMS SOP-101-A_2 1X Inactivation Saline Solution Prep - Table Form category: guides subcategory: qms-sops tags: source_file_type: gdoc xfile_type: docx gfile_url: https://docs.google.com/document/d/1YiosM1yHUjlkwg_aE_j-xyOyT_9hJk_tyT2OU4qDjW8 xfile_github_download_url: https://raw.githubusercontent.com/FocusOnFoundationsNonprofit/floodlamp-archive/main/guides/qms-sops/SOP-101-A_2%201X%20Inactivation%20Saline%20Solution%20Prep%20-%20Table%20Form.docx pdf_gdrive_url: https://drive.google.com/file/d/1P9W2Zu3esKwstzSynPQJKBlZu_PPjR0S pdf_github_url: https://github.com/FocusOnFoundationsNonprofit/floodlamp-archive/blob/main/guides/qms-sops/SOP-101-A_2%201X%20Inactivation%20Saline%20Solution%20Prep%20-%20Table%20Form.pdf conversion_input_file_type: docx conversion: pandoc license: CC BY 4.0 - https://creativecommons.org/licenses/by/4.0/ tokens: 457 words: 266 notes: summary_short: The FloodLAMP QMS SOP-101-A_2 provides a table-form protocol and log for preparing 1X Inactivation Saline Solution by diluting 100X Inactivation Solution 1:100 into saline with specified vortexing/mixing steps. It includes fields for lot numbers, freeze/thaw and expiration tracking, “put in use” documentation, and a preparation log table capturing volumes and IDs (1X_MMDD-1). The SOP sets the 1X solution expiration at 2 weeks after preparation. CONTENT FloodLAMP Biotechnologies **Document Number:** SOP-101-A_2 **Effective Date:** 08/24/2022 **Version:** 2 ***INTERNAL TITLE:*** 1X Inactivation Saline Solution Prep - Table Form ## 100X Inactivation Solution Lot (-MMDD): Freeze EXP (MM-YY): Thaw EXP (+3 months): ## Put New in Use: Location: Name: Date and Time: Log Link: SOP-101-B_1 {SITE} Name: Date and Time: ## 1X Inactivation Saline Solution Prep Protocol: 1\) Vortex 100X Inactivation Solution for 10 sec. 2\) Add 1/100 volume of 100X Inactivation Solution to Saline. 3\) Vortex for 10 sec, Invert 3 times, and Vortex for 10 more sec. \* If making Inactivation Solution in a bottle, shake instead of vortexing 4\) Label tube with 1XISS ID (1X\_MMDD-1). 5\) EXPIRATION of 1X Inactivation Saline Solution: 2 weeks after prep. | **Name** | **Date + Time** | **1XISS ID**
**(1X_MMDD-1)** | **Saline**
**Lot + Exp** | **Saline**
**mL** | **100XISS**
**µL** | |----------|----------------|-------------------|-------------------|----------------|-----------------| | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | ||