METADATA last updated: 2025-12-14 RT after BA fixed inconsistencies file_name: SOP-105-B_1 QuickColor Test Signage.md file_date: 2022-08-26 title: FloodLAMP QMS SOP-105-B_1 QuickColor Test Signage category: guides subcategory: qms-sops tags: source_file_type: gdoc xfile_type: docx gfile_url: https://docs.google.com/document/d/1IO3MjHxGCzMMlYJsnHvZyzYv-ohpRolbGJksY8S08-E xfile_github_download_url: https://raw.githubusercontent.com/FocusOnFoundationsNonprofit/floodlamp-archive/main/guides/qms-sops/SOP-105-B_1%20QuickColor%20Test%20Signage.docx pdf_gdrive_url: https://drive.google.com/file/d/1RR_V3e6fn0g7xCESHnX3t-6VYQi3r1NU pdf_github_url: https://github.com/FocusOnFoundationsNonprofit/floodlamp-archive/blob/main/guides/qms-sops/SOP-105-B_1%20QuickColor%20Test%20Signage.pdf conversion_input_file_type: docx conversion: pandoc license: CC BY 4.0 - https://creativecommons.org/licenses/by/4.0/ tokens: 679 words: 309 notes: summary_short: The "SOP-105-B_1 QuickColor Test Signage" document is a print-ready, step-by-step bench guide for running the QuickColor assay from sample intake through reporting results. It is 8 pages that are meant to be placed on the walls or tables at the corresponding reaction stations, with large font and simple instructions. It summarizes key workflow actions and parameters, including 1XISS dilution ratios, inactivation heating/cooling times for water bath or dry heat block, reaction mix component volumes for common reaction counts, and 65 °C amplification timing. The signage also highlights contamination-control reminders (cleaning tubes, one tube open at a time) and imaging/logging instructions for reporting. CONTENT FloodLAMP Biotechnologies **Document Number:** SOP-105-B_1 **Effective Date:** 08/26/2022 **Version:** 1 ***INTERNAL TITLE:*** QuickColor Test Signage Print Format 1\) Intake Sample Tubes --- - **Clean tubes - spray wipe with ethanol/alcohol and wipe tubes** - **Intake with App (can also do during Amp heating)** 2\) Make 1X Inactivation Saline Solution --- - **Add 100X Inactivation Solution to Saline** | | | | |:----------------------:|:-----------------------:|:-----------------------:| | **Saline**
**5 mL** | **Saline**
**15 mL** | **Saline**
**50 mL** | | **100X IS**
**50 μL** | **100X IS**
**150 μL** | **100X IS**
**500 μL** | || 3\) Add 1X Inactivation Saline Solution to Sample Tubes --- - **Add 1mL of inactivation saline solution (1XISS) to each sample tube** - **One sample tube open at a time** 4\) Heat to Inactivate Samples --- - **Water Bath (set at 99°C)** **Heat 8 mintues** **Cool 10 minutes** - **Dry Heat Block for 1.5mL sample tubes (set at 95°C)** **Heat 5 mintues** **Cool 5 minutes** 5\) Make Reaction Mix --- - **Add CLAMP MM and Primer Soln (PGS) to 1.5mL tube** - **Aliquot into Strip8 tubes or 96 well PCR Plate** | | | | | |:--------:|:--------:|:--------:|:--------:| | **8 Rxns** | **16 Rxns** | **24 Rxns** | **48 Rxns** | | **PGS**
**92 μL** | **PGS**
**183 μL** | **PGS**
**275 μL** | **PGS**
**550 μL** | | **CLAMP MM**
**109 μL** | **CLAMP MM**
**218 μL** | **CLAMP MM**
**328 μL** | **CLAMP MM**
**655 μL** | || 6\) Add Sample to Reaction --- - **Add 2μL from each sample tube to corresponding well** - **Pipet up and down 5 times, blowout in liquid, tip touch** 7\) Heat Amp Reactions --- - **Heat at 65ºC for 25 min** - **Set personal timer (on phone) for 24 min** 8\) Log and Report Results --- - **Let samples cool for at least 1 minute before taking photo** - **Crop and apply vivid filter** - **Log Amp Run Form with QR code link**