METADATA last updated: 2025-12-14 RT after BA fixed inconsistencies file_name: SOP-105-B_1 QuickColor Test Signage.md file_date: 2022-08-26 title: FloodLAMP QMS SOP-105-B_1 QuickColor Test Signage category: guides subcategory: qms-sops tags: source_file_type: gdoc xfile_type: docx gfile_url: https://docs.google.com/document/d/1IO3MjHxGCzMMlYJsnHvZyzYv-ohpRolbGJksY8S08-E xfile_github_download_url: https://raw.githubusercontent.com/FocusOnFoundationsNonprofit/floodlamp-archive/main/guides/qms-sops/SOP-105-B_1%20QuickColor%20Test%20Signage.docx pdf_gdrive_url: https://drive.google.com/file/d/1RR_V3e6fn0g7xCESHnX3t-6VYQi3r1NU pdf_github_url: https://github.com/FocusOnFoundationsNonprofit/floodlamp-archive/blob/main/guides/qms-sops/SOP-105-B_1%20QuickColor%20Test%20Signage.pdf conversion_input_file_type: docx conversion: pandoc license: CC BY 4.0 - https://creativecommons.org/licenses/by/4.0/ tokens: 679 words: 309 notes: summary_short: The FloodLAMP QMS SOP-105-B_1 QuickColor Test Signage is a print-ready, step-by-step bench guide for running the QuickColor assay from sample intake through reporting results. It summarizes key workflow actions and parameters, including 1XISS dilution ratios, inactivation heating/cooling times for water bath or dry heat block, reaction mix component volumes for common reaction counts, and 65 °C amplification timing. The signage also highlights contamination-control reminders (cleaning tubes, one tube open at a time) and imaging/logging instructions for reporting. CONTENT FloodLAMP Biotechnologies **Document Number:** SOP-105-B_1 **Effective Date:** 08/26/2022 **Version:** 1 ***INTERNAL TITLE:*** QuickColor Test Signage Print Format 1\) Intake Sample Tubes --- - **Clean tubes - spray wipe with ethanol/alcohol and wipe tubes** - **Intake with App (can also do during Amp heating)** 2\) Make 1X Inactivation Saline Solution --- - **Add 100X Inactivation Solution to Saline** | | | | |:----------------------:|:-----------------------:|:-----------------------:| | **Saline**
**5 mL** | **Saline**
**15 mL** | **Saline**
**50 mL** | | **100X IS**
**50 μL** | **100X IS**
**150 μL** | **100X IS**
**500 μL** | || 3\) Add 1X Inactivation Saline Solution to Sample Tubes --- - **Add 1mL of inactivation saline solution (1XISS) to each sample tube** - **One sample tube open at a time** 4\) Heat to Inactivate Samples --- - **Water Bath (set at 99°C)** **Heat 8 mintues** **Cool 10 minutes** - **Dry Heat Block for 1.5mL sample tubes (set at 95°C)** **Heat 5 mintues** **Cool 5 minutes** 5\) Make Reaction Mix --- - **Add CLAMP MM and Primer Soln (PGS) to 1.5mL tube** - **Aliquot into Strip8 tubes or 96 well PCR Plate** | | | | | |:--------:|:--------:|:--------:|:--------:| | **8 Rxns** | **16 Rxns** | **24 Rxns** | **48 Rxns** | | **PGS**
**92 μL** | **PGS**
**183 μL** | **PGS**
**275 μL** | **PGS**
**550 μL** | | **CLAMP MM**
**109 μL** | **CLAMP MM**
**218 μL** | **CLAMP MM**
**328 μL** | **CLAMP MM**
**655 μL** | || 6\) Add Sample to Reaction --- - **Add 2μL from each sample tube to corresponding well** - **Pipet up and down 5 times, blowout in liquid, tip touch** 7\) Heat Amp Reactions --- - **Heat at 65ºC for 25 min** - **Set personal timer (on phone) for 24 min** 8\) Log and Report Results --- - **Let samples cool for at least 1 minute before taking photo** - **Crop and apply vivid filter** - **Log Amp Run Form with QR code link**