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last updated: 2025-12-14 RT after BA fixed inconsistencies
file_name: SDS - EDTA.md
file_date: 2018-01-18
title: Safety Data Sheet - EDTA
category: guides
subcategory: sds
tags: 
source_file_type: pdf
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pdf_gdrive_url: https://drive.google.com/file/d/1sUCW4idcd041WWuOUOgRoQvt1X5mqoNY
pdf_github_url: https://github.com/FocusOnFoundationsNonprofit/floodlamp-archive/blob/main/guides/sds/SDS%20-%20EDTA.pdf
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license: public domain
tokens: 3537
words: 2062
notes: 
summary_short: The Safety Data Sheet for EDTA (ethylenediaminetetraacetic acid, CAS 60-00-4) summarizes hazards, safe handling/storage, exposure controls, and emergency measures for laboratory use. It identifies inhalation (dust/mist) and eye irritation risks with potential respiratory effects from repeated exposure, and provides spill cleanup, fire response, and disposal guidance. The sheet also notes aquatic toxicity, transport as not regulated, and key regulatory/inventory listings and reportable quantity thresholds.


CONTENT

***INTERNAL TITLE:*** ThermoFisher Scientific - Safety Data Sheet

**Creation Date** 02-Jan-2015 
**Revision Date** 18-Jan-2018 
**Revision Number** 6

## 1. Identification
**Product Name:** Ethylenediamine Tetraacetic Acid

**Cat No.:** BP118-500; E478-1; E478-10; E478-500; NC1065691; XXBP118-10KG; NC1163901; XXE478-12KG; NC1253743

**CAS-No:** 60-00-4

**Synonyms:** 
- 3,6-Diazaoctanedioic acid, 3,6-bis(carboxymethy)
- Acetic acid, (Ethylenedinitrilo)tetraacetic acid
- EDTA
- Edetic acid
- Diaminoethanetetraacetic acid

**Recommended Use:** Laboratory chemicals

**Uses advised against:** Not for food, drug, pesticide or biocidal product use

**Details of the supplier of the safety data sheet:**

**Company:**
Fisher Scientific
One Reagent Lane
Fair Lawn, NJ 07410
Tel: (201) 796-7100

**Emergency Telephone Number:**
CHEMTRECÒ, Inside the USA: 800-424-9300
CHEMTRECÒ, Outside the USA: 001-703-527-3887

## 2. Hazard(s) identification
### Classification
This chemical is considered hazardous by the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200)
|  |  |
|--------|----------|
| Acute Inhalation Toxicity - Dusts and Mists | Category 4 |
| Serious Eye Damage/Eye Irritation | Category 2 |
| Specific target organ toxicity - (repeated exposure) | Category 2 |
| Target Organs - Respiratory system. | - |
||

### Label Elements
**Signal Word:** Warning

**Hazard Statements:**
- Causes serious eye irritation
- Harmful if inhaled
- May cause damage to organs through prolonged or repeated exposure

**Precautionary Statements:**
*Prevention:*
- Use only outdoors or in a well-ventilated area
- Wash face, hands and any exposed skin thoroughly after handling
- Wear eye/face protection
- Do not breathe dust/fume/gas/mist/vapors/spray

*Response:*
- Get medical attention/advice if you feel unwell

*Inhalation:*
- IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
- Call a POISON CENTER or doctor/physician if you feel unwell

*Eyes:*
- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- If eye irritation persists: Get medical advice/attention

*Disposal:*
- Dispose of contents/container to an approved waste disposal plant

**Hazards not otherwise classified (HNOC):** None identified

## 3. Composition/Information on Ingredients
| Component | CAS-No | Weight % |
|-----------|--------|----------|
| Ethylenediamine tetraacetic acid (EDTA) | 60-00-4 | > 99 |
||

## 4. First-aid measures
**General Advice:** If symptoms persist, call a physician.

**Eye Contact:** Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention if irritation persists.

**Skin Contact:** Wash off immediately with plenty of water for at least 15 minutes. If skin irritation persists, call a physician.

**Inhalation:** Move to fresh air. If not breathing, give artificial respiration. Get medical attention if symptoms occur.

**Ingestion:** Clean mouth with water and drink afterwards plenty of water. Get medical attention if symptoms occur.

**Most important symptoms and effects:** None reasonably foreseeable.

**Notes to Physician:** Treat symptomatically

## 5. Fire-fighting measures
**Suitable Extinguishing Media:** Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.

**Unsuitable Extinguishing Media:** No information available

**Flash Point:** Not applicable

**Method:** No information available

**Autoignition Temperature:** Not applicable 200 °C / 392 °F

**Explosion Limits:**
- Upper: No data available
- Lower: No data available
- Sensitivity to Mechanical Impact: No information available
- Sensitivity to Static Discharge: No information available

**Specific Hazards Arising from the Chemical:** Thermal decomposition can lead to release of irritating gases and vapors. Keep product and empty container away from heat and sources of ignition.

**Hazardous Combustion Products:** Nitrogen oxides (NOx), Carbon monoxide (CO), Carbon dioxide (CO2)

**Protective Equipment and Precautions for Firefighters:** As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full protective gear.

**NFPA:**
- Health: 2
- Flammability: 1
- Instability: 0
- Physical hazards: N/A

## 6. Accidental release measures
**Personal Precautions:** Use personal protective equipment. Ensure adequate ventilation. Avoid dust formation.

**Environmental Precautions:** Do not flush into surface water or sanitary sewer system.

**Methods for Containment and Clean Up:** Sweep up or vacuum up spillage and collect in suitable container for disposal. Keep in suitable, closed containers for disposal.

## 7. Handling and storage
**Handling:** Wear personal protective equipment. Ensure adequate ventilation. Avoid dust formation. Do not get in eyes, on skin, or on clothing. Avoid ingestion and inhalation.

**Storage:** Keep in a dry, cool and well-ventilated place. Keep container tightly closed.

## 8. Exposure controls / personal protection
**Exposure Guidelines:** This product does not contain any hazardous materials with occupational exposure limits established by the region-specific regulatory bodies.

**Engineering Measures:** Ensure adequate ventilation, especially in confined areas. Ensure that eyewash stations and safety showers are close to the workstation location.

**Personal Protective Equipment:**
*Eye/face Protection:* Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.

*Skin and body protection:* Long sleeved clothing.

*Respiratory Protection:* Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

*Hygiene Measures:* Handle in accordance with good industrial hygiene and safety practice.

## 9. Physical and chemical properties
|  |  |
|----------|-------|
| Physical State | Powder Solid |
| Appearance | White |
| Odor | Odorless |
| Odor Threshold | No information available |
| pH | 2.5 10 g/L (23°C) |
| Melting Point/Range | 220 °C / 428 °F |
| Boiling Point/Range | No information available |
| Flash Point | Not applicable |
| Evaporation Rate | Not applicable |
| Flammability (solid,gas) | No information available |
| Flammability or explosive limits | Upper: No data available<br>Lower: No data available |
| Vapor Pressure | 0.013 hPa @ 20 °C |
| Vapor Density | Not applicable |
| Specific Gravity | 0.86 @ 20°C |
| Solubility | Slightly soluble in water |
| Partition coefficient; n-octanol/water | No data available |
| Autoignition Temperature | Not applicable 200 °C / 392 °F |
| Decomposition Temperature | > 150°C |
| Viscosity | Not applicable |
| Molecular Formula | C10 H16 N2 O8 |
| Molecular Weight | 292.23 |
||

## 10. Stability and reactivity
**Reactive Hazard:** None known, based on information available

**Stability:** Stable under normal conditions.

**Conditions to Avoid:** Avoid dust formation. Incompatible products. Excess heat.

**Incompatible Materials:** Strong oxidizing agents, Strong bases, Metals, copper

**Hazardous Decomposition Products:** Nitrogen oxides (NOx), Carbon monoxide (CO), Carbon dioxide (CO2)

**Hazardous Polymerization:** Hazardous polymerization does not occur.

**Hazardous Reactions:** None under normal processing.

## 11. Toxicological information
### Acute Toxicity
**Product Information**
**Component Information**
| Component | LD50 Oral | LD50 Dermal | LC50 Inhalation |
|-----------|-----------|-------------|------------------|
| Ethylenediamine tetraacetic acid (EDTA) | 4500 mg/kg ( Rat )<br>>2000 mg/kg ( Rat ) | Not listed | 1 mg/l (rat) |
||

**Toxicologically Synergistic Products:** No information available

### Delayed and immediate effects as well as chronic effects from short and long-term exposure
**Irritation:** Irritating to eyes

**Sensitization:** No information available

**Carcinogenicity:** The table below indicates whether each agency has listed any ingredient as a carcinogen.
| Component | CAS-No | IARC | NTP | ACGIH | OSHA | Mexico |
|-----------|--------|------|-----|-------|------|--------|
| Ethylenediamine tetraacetic acid (EDTA) | 60-00-4 | Not listed | Not listed | Not listed | Not listed | Not listed |
||

**Mutagenic Effects:** No information available

**Reproductive Effects:** No information available.

**Developmental Effects:** No information available.

**Teratogenicity:** No information available.

**STOT - single exposure:** None known

**STOT - repeated exposure:** Respiratory system

**Aspiration hazard:** No information available

**Symptoms / effects, both acute and delayed:** No information available

**Endocrine Disruptor Information:** No information available

**Other Adverse Effects:** The toxicological properties have not been fully investigated.

## 12. Ecological information
### Ecotoxicity
Contains a substance which is: The product contains following substances which are hazardous for the environment. Toxic to aquatic organisms.
| Component | Freshwater Algae | Freshwater Fish | Microtox | Water Flea |
|-----------|------------------|-----------------|----------|------------|
| Ethylenediamine tetraacetic acid (EDTA) | EC50: = 1.01 mg/L, 72h (Desmodesmus subspicatus) | LC50: 44.2 - 76.5 mg/L, 96h static (Pimephales promelas)<br>LC50: 34 - 62 mg/L, 96h static (Lepomis macrochirus) | Not listed | EC50: = 113 mg/L, 48h Static (Daphnia magna) |
||

**Persistence and Degradability:** Soluble in water Persistence is unlikely based on information available.

**Bioaccumulation/ Accumulation:** No information available.

**Mobility:** Will likely be mobile in the environment due to its water solubility.

## 13. Disposal considerations
**Waste Disposal Methods:** Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. Chemical waste generators must also consult local, regional, and national hazardous waste regulations to ensure complete and accurate classification.

## 14. Transport information
**DOT:** Not regulated

**TDG:** Not regulated

**IATA:** Not regulated

**IMDG/IMO:** Not regulated

## 15. Regulatory information
**International Inventories:**
| Component | TSCA | DSL | NDSL | EINECS | ELINCS | NLP | PICCS | ENCS | AICS | IECSC | KECL |
|-----------|------|-----|------|--------|--------|-----|-------|------|------|-------|------|
| Ethylenediamine tetraacetic acid (EDTA) | X | X | - | 200-449-4 | - |  | X | X | X | X | X |
||

**Legend:**
X - Listed
E - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.
F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA.
N - Indicates a polymeric substance containing no free-radical initiator in its inventory name but is considered to cover the designated polymer made with any free-radical initiator regardless of the amount used.
P - Indicates a commenced PMN substance
R - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.
S - Indicates a substance that is identified in a proposed or final Significant New Use Rule
T - Indicates a substance that is the subject of a Section 4 test rule under TSCA.
XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data Base Production and Site Reports (40 CFR 710(B).
Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater.
Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specified list of low concern reactants that comprises one of the eligibility criteria for the exemption rule.

### U.S. Federal Regulations
**TSCA 12(b):** Not applicable

**SARA 313:** Not applicable

**SARA 311/312 Hazard Categories:** See section 2 for more information

**CWA (Clean Water Act):**
| Component | CWA - Hazardous Substances | CWA - Reportable Quantities | CWA - Toxic Pollutants | CWA - Priority Pollutants |
|-----------|----------------------------|-----------------------------|-----------------------|---------------------------|
| Ethylenediamine tetraacetic acid (EDTA) | X | 5000 lb | - | - |
||

**Clean Air Act:** Not applicable

**OSHA Occupational Safety and Health Administration:** Not applicable

**CERCLA:**
| Component | Hazardous Substances RQs | CERCLA EHS RQs |
|-----------|--------------------------|-----------------|
| Ethylenediamine tetraacetic acid (EDTA) | 5000 lb | - |
||

**California Proposition 65:** This product does not contain any Proposition 65 chemicals

**U.S. State Right-to-Know Regulations:**
| Component | Massachusetts | New Jersey | Pennsylvania | Illinois | Rhode Island |
|-----------|---------------|------------|--------------|----------|---------------|
| Ethylenediamine tetraacetic acid (EDTA) | X | X | X | - | - |
||

**U.S. Department of Transportation:**
- Reportable Quantity (RQ): N
- DOT Marine Pollutant: N
- DOT Severe Marine Pollutant: N

**U.S. Department of Homeland Security:** This product does not contain any DHS chemicals.

### Other International Regulations
**Mexico - Grade:** No information available

## 16. Other information
**Prepared By:** 
Regulatory Affairs
Thermo Fisher Scientific
Email: EMSDS.RA@thermofisher.com

**Creation Date:** 02-Jan-2015
**Revision Date:** 18-Jan-2018
**Print Date:** 18-Jan-2018
**Revision Summary:** This document has been updated to comply with the US OSHA HazCom 2012 Standard replacing the current legislation under 29 CFR 1910.1200 to align with the Globally Harmonized System of Classification and Labeling of Chemicals (GHS).

**Disclaimer:**
The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. The information
relates only to the specific material designated and may not be valid for such material used in combination with any other
materials or in any process, unless specified in the text

End of SDS