METADATA last updated: 2025-12-14 RT updated metadata file_name: Abrome Site Checklists INTERNAL COPY BEFORE SHARING.md file_date: 2022-09-04 title: Testing Site Checklists category: guides subcategory: test-site tags: source_file_type: gdoc xfile_type: docx gfile_url: https://docs.google.com/document/d/1Plnksj-OwJzUV9f0WIvhQncd_pBCS50HJuw-lSi7IqM xfile_github_download_url: https://raw.githubusercontent.com/FocusOnFoundationsNonprofit/floodlamp-archive/main/guides/test-site/Abrome%20Site%20Checklists%20INTERNAL%20COPY%20BEFORE%20SHARING.docx pdf_gdrive_url: https://drive.google.com/file/d/1jy34vLE5VOkjL2RXIq_ATPv92UBJhfgP pdf_github_url: https://github.com/FocusOnFoundationsNonprofit/floodlamp-archive/blob/main/guides/test-site/Abrome%20Site%20Checklists%20INTERNAL%20COPY%20BEFORE%20SHARING.pdf conversion_input_file_type: docx conversion: pandoc license: CC BY 4.0 - https://creativecommons.org/licenses/by/4.0/ tokens: 1272 words: 704 notes: summary_short: Provides the complete operational checklists for running FloodLAMP surveillance tests at testing sites, covering daily test procedures (equipment warm-up, reagent verification, sample processing, result communication), weekly maintenance, and ongoing lab readiness standards. It standardizes the testing workflow to ensure accurate results, proper reagent handling, and timely follow-up on positive/inconclusive pools. This was introduced at the pilot site Abrome. CONTENT ## Testing Checklist - Abrome v1.1 Last updated: 9-4-2022 by Randy True Direct any questions to support@floodlamp.bio ### Upon Arrival - Water bath & amp heater turned on ***at least one hour*** before starting to process samples - Can add boiling water from kettle to speed up the process - Change water bath water weekly ### During Water Bath Heating - Review freezer sensor data to ensure no thawing occurred. - Prepare fresh 10% Bleach Solution, preferably daily, but at least every 3 days. - Clean Inactivation Table thoroughly - first with 10% bleach, then with 70% ethanol or IPA. - Set up Inactivation Table. - Verify sufficient 1XISS is prepared (minimum 1 mL per sample, plus extra 5mL) **AND < 2 weeks old** - Otherwise prepare 1XISS: [SOP-101-A\_2 1X Inactivation Saline Solution Prep - Table Form - Abrome.pdf](https://drive.google.com/file/d/1OLXQShwg3ecKP3Os5c2JDuG3UCbrbuiQ/view?usp=sharing) - Verify sufficient aliquots of RM (one per sample + two for controls, plus extra) **AND not expired** (< 4 weeks old, no visible discoloration) - Otherwise prepare RM: [SOP-102-A\_2 Reaction Mix Prep - Table Form - Abrome.pdf](https://drive.google.com/file/d/1Qv8Bk1rReWMlm9Z3X3sK9cdq9YcxL8Vt/view?usp=sharing) - RM in strips-of-8 tubes to be used today can be kept in refrigerator until use - Any additional RM in strips-of-8 tubes should be placed immediately in freezer - Verify that caps are tightly sealed! - Verify sufficient test consumables for usage (1 mL tips, 10 uL tips, gloves, wipes, aluminum foil) - Intake collection tubes, preferably prior to the cutoff time, or during Amp - Ideal would be to intake tubes as people return them, so as to address the uncollected tubes (tubes returned without the collection step completed) - Ideally verify receipt of all expected collection tubes from students and staff - Note any anomalies (e.g., uncollected tubes) - Double check water bath is up to temperature (at least 95°C) before starting to process samples ### Running Test - Turn off air purifiers before beginning the test procedure. Keep the door closed. - Tie back hair (if applicable). - Run test: - Longer Training Amp Run Form: [SOP-104-A\_1 Test Training - Run Form - Abrome.pdf](https://drive.google.com/file/d/1KoCXZzsHXKmg85mVdKIEbzHbFr20deDd/view?usp=sharing) - Standard Amp Run Form: [SOP-103-A\_1 Amplification - Run Form - Abrome.pdf](https://drive.google.com/file/d/1REe5TKDHvmMoiBQUJHJOri5CR1VhO0qk/view?usp=sharing) - Rerun test on any positive and inconclusive sample(s) in triplicate using the same inactivated sample(s) kept in the refrigerator. - After running the test and any reruns, if any pools have returned positive or inconclusive results: - Communicate ***referrals*** to participants in those pools: - [Surveillance Program Results Communications Guidelines.pdf](https://drive.google.com/file/d/1p7oEPNAbIhwoYzjoO3FkHD6Oiw-Z1sxI/view?usp=sharing) - Enact your internal protocols accordingly. ### Cleanup - Return cold blocks to the freezer. - Return pipettes, tip boxes, racks. - Check that all thawed inactivation solution bottles & vials are covered in foil. - Discard sample tubes from refrigerator (neg. in trash, pos. in bag in bottom freezer). - Turn off heaters. - Empty tip buckets and clean them with bleach and ethanol. ## Weekly Checklist - Abrome - v1.1 Last updated: 9-2-2022 by Gary Withey Direct any questions to support@floodlamp.bio - Change water in water bath weekly. - Preferable to use distilled water. - Update complete Inventory according to schedule as agreed with FloodLAMP staff. - Check Expiration dates of reagents. - Empty trash bins. - OPTIONALLY: Prepare reaction tubes for the week, and freeze. ## State Of Readiness Checklist - Abrome - v1.1 Last updated: 9-2-2022 by Gary Withey Direct any questions to support@floodlamp.bio The following should be in place at all times to ensure smooth operation of the lab: - Glove boxes BOTH in assay prep area AND amplification area (keep separate glove supplies). - Wipes present (one active, plus extra on supply shelf). - Empty Tip box filled with strip tubes (fill tip box + half source box). - Falcon Tubes Filled with strip tube caps (two full falcon tubes, + half source box). - 1.5mL tubes closed and in cleaned, labeled cryobox (1 active box, Next box fully ready). - At least 5 blank Amp run forms, and at least 1 spare of both table forms (1X ISS & RM). - Pens and markers clean and in proper place. - Pens tested and functional. - Spare tube of inactivation solution in freezer. - Freezer thermometer tested and functional. - Old non-significant samples clear from fridge. - Tip buckets empty.