METADATA
last updated: 2026-03-06 by BA
file_name: XPrize FloodLAMP Submission - Part 7 Targets.md
file_date: 2020-09-08
title: FloodLAMP XPRIZE Submission - Part 7 Targets
category: various
subcategory: xprize
tags:
source_file_type: gsheet
xfile_type: xlsx
gfile_url: https://docs.google.com/spreadsheets/d/1K6wkOvyBhdVZBEKIYphTfM18wlJ0xKoG/
xfile_github_download_url: https://raw.githubusercontent.com/FocusOnFoundationsNonprofit/floodlamp-archive-wip/main/various/xprize/XPrize%20FloodLAMP%20Submission%20-%20Part%207%20Targets.xlsx
pdf_gdrive_url: NA
pdf_github_url: NA
conversion_input_file_type: xlsx
conversion: msmid
license: CC BY 4.0 - https://creativecommons.org/licenses/by/4.0/
tokens: 584
words: 215
notes:
summary_short: FloodLAMP's XPRIZE submission Part 7 Targets spreadsheet lists the 18 LAMP primer sequences targeting ORF1 (As1e set) and N gene (N2 set), plus 6 RNaseP internal control primers used in the FloodLAMP SARS-CoV-2 assay.


CONTENT

## FloodLAMP Targets
### Primer Sequences OR Antigen target/antibody
| Target Number | Target Gene | Sequence/Antibody |
| --- | --- | --- |
| Numeric | Text | Text |
| 1 | ORF1 As1e_FIP | TCAGCACACAAAGCCAAAAATTTATTTTTCTGTGCAAAGGAAATTAAGGAG |
| 2 | ORF1 As1e_BIP | TATTGGTGGAGCTAAACTTAAAGCCTTTTCTGTACAATCCCTTTGAGTG |
| 3 | ORF1 As1_F3 | CGGTGGACAAATTGTCAC |
| 4 | ORF1 As1_B3 | CTTCTCTGGATTTAACACACTT |
| 5 | ORF1 As1_LF | TTACAAGCTTAAAGAATGTCTGAACACT |
| 6 | ORF1 As1_LB | TTGAATTTAGGTGAAACATTTGTCACG |
| 7 | N N2-FIP | TTCCGAAGAACGCTGAAGCGGAACTGATTACAAACATTGGCC |
| 8 | N N2-BIP | CGCATTGGCATGGAAGTCACAATTTGATGGCACCTGTGTA |
| 9 | N N2-F3 | ACCAGGAACTAATCAGACAAG |
| 10 | N N2-B3 | GACTTGATCTTTGAAATTTGGATCT |
| 11 | N N2-LF | GGGGGCAAATTGTGCAATTTG |
| 12 | N N2-LB | CTTCGGGAACGTGGTTGACC |
| 13 | RNaseP-F3 | TTGATGAGCTGGAGCCA |
| 14 | RNaseP-B3 | CACCCTCAATGCAGAGTC |
| 15 | RNaseP-FIP | GTGTGACCCTGAAGACTCGGTTTTAGCCACTGACTCGGATC |
| 16 | RNaseP-BIP | CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACATGGCTCTGGTC |
| 17 | RNaseP-LF | ATGTGGATGGCTGAGTTGTT |
| 18 | RNaseP-LB | CATGCTGAGTACTGGACCTC |
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We are currently using the As1e and N2 combined for maximum sensitivity.

We may move to running them in 2 separate reactions to have independent results for confidence in positives.

RNAseP is standard but we plan to move to an RNA only internal control.