METADATA last updated: 2026-03-06 by BA file_name: XPrize FloodLAMP Submission - Part 8 Results.md file_date: 2020-09-08 title: FloodLAMP XPRIZE Submission - Part 8 Results category: various subcategory: xprize tags: source_file_type: gsheet xfile_type: xlsx gfile_url: https://docs.google.com/spreadsheets/d/1A7XmTQysodHBUUbLf-cBhAZOQLpkIIGV/ xfile_github_download_url: https://raw.githubusercontent.com/FocusOnFoundationsNonprofit/floodlamp-archive-wip/main/various/xprize/XPrize%20FloodLAMP%20Submission%20-%20Part%208%20Results.xlsx pdf_gdrive_url: NA pdf_github_url: NA conversion_input_file_type: xlsx conversion: msmid license: CC BY 4.0 - https://creativecommons.org/licenses/by/4.0/ tokens: 2146 words: 1287 notes: summary_short: FloodLAMP's XPRIZE submission Part 8 Results spreadsheet provides test data from multiple experimental runs including blinded real saliva samples spiked with ZeptoMetrix inactivated virions, Twist RNA limit-of-detection runs at 2mL volume, and real nasal/saliva sample screening, all using As1e and N2 combined primer sets with visual binary readout. CONTENT ## FloodLAMP Results ### Results | | | | | | | | | | | |---------------------------------------------------------------------------------------------------------------------------------------------------------------|-------------|---------------------------|---------------|-------------|--------------|--------------|---------------|-----------------------------------------------|------------------------------| | Sample # | Known Value | Sample Type | Sample Source | Known Value | Known Units | Result Value | Result Units | Result Interpretation | Notes | | Numeric | Text | Text | Text | Numeric | Text | Numeric | Text | Text | Text | || Blinded real saliva samples, spiked with zeptometrix inactivated virions into raw sample before any processing | | | | | | | | | | | |---------------------------------------------------------------------------------------------------------------------------------------------------------------|-------------|---------------------------|---------------|-------------|--------------|--------------|---------------|-----------------------------------------------|------------------------------| | Run#39-1 | Negative | Real Unspiked | Saliva | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-2 | Negative | Real Unspiked | Saliva | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-3 | Positive | Real, Raw Zepto Spiked | Saliva | 5E+03 | virions/ml | Positive | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-4 | Negative | Real Unspiked | Saliva | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-5 | Positive | Real, Raw Zepto Spiked | Saliva | 2E+04 | virions/ml | Positive | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-6 | Negative | Real Unspiked | Saliva | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-7 | Negative | Real Unspiked | Saliva | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-8 | Positive | Real, Raw Zepto Spiked | Saliva | 1E+04 | virions/ml | Positive | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-9 | Negative | Real Unspiked | Saliva | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-10 | Negative | Real Unspiked | Saliva | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-NTC | Negative | Control | PBS | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#39-TWC | Positive | Contrived | Saliva | 2,500 | copies (est) | Positive | Visual binary | Correct | As1e and N2 Primer Set Combo | || Twist LOD run on 2ml volume of inactivated sample, Twist positive control RNA spiked after sample inactivation before purification, same results in duplicate | | | | | | | | | | | |---------------------------------------------------------------------------------------------------------------------------------------------------------------|-------------|---------------------------|---------------|-------------|--------------|--------------|---------------|-----------------------------------------------|------------------------------| | Run#37-8K | Positive | Contrived | Saliva | 4,000 | copies (est) | Positive | Visual binary | At or above LOD | As1e and N2 Primer Set Combo | | Run#37-4K | Positive | Contrived | Saliva | 2,000 | copies (est) | Positive | Visual binary | At or above LOD | As1e and N2 Primer Set Combo | | Run#37-2K | Positive | Contrived | Saliva | 1,000 | copies (est) | Positive | Visual binary | At or above LOD | As1e and N2 Primer Set Combo | | Run#37-1K | Positive | Contrived | Saliva | 500 | copies (est) | Negative | Visual binary | Below LOD | As1e and N2 Primer Set Combo | | Run#37-0 | Negative | Control | Saliva | 0 | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#37-0 | Positive | Control (Internal RNAseP) | Saliva | HIGH | | Positive | Visual binary | Correct | RNAseP Primers | | Run#37-NTC | Negative | Control | PBS | 0 | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#37-NTC | Negative | Control (Internal RNAseP) | PBS | 0 | | Negative | Visual binary | Correct | RNAseP Primers | || Real Sample run on individual for a screen, 2 nasal samples split and 25K zeptos spiked into 2.5ml | | | | | | | | | | | |---------------------------------------------------------------------------------------------------------------------------------------------------------------|-------------|---------------------------|---------------|-------------|--------------|--------------|---------------|-----------------------------------------------|------------------------------| | Run#86-1A | ? | Real | Nasal | | | Negative | Visual binary | No Finding of Potential Clinical Significance | As1e and N2 Primer Set Combo | | Run#86-1B | Positive | Real, Raw Zepto Spiked | Nasal | 1E+04 | virions/ml | Positive | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#86-2A | Negative | Real | Nasal | | | Negative | Visual binary | No Finding of Potential Clinical Significance | As1e and N2 Primer Set Combo | | Run#86-2B | Positive | Real, Raw Zepto Spiked | Nasal | 1E+04 | virions/ml | Positive | Visual binary | Correct | As1e and N2 Primer Set Combo | | Run#86-3 | Negative | Real | Nasal | | | Negative | Visual binary | No Finding of Potential Clinical Significance | As1e and N2 Primer Set Combo | | Run#86-4 | Negative | Real | Nasal | | | Negative | Visual binary | No Finding of Potential Clinical Significance | As1e and N2 Primer Set Combo | | Run#86-5 | ? | Real | Saliva | | | Negative | Visual binary | No Finding of Potential Clinical Significance | As1e and N2 Primer Set Combo | | Run#86-NTC | Negative | Control | PBS | | | Negative | Visual binary | Correct | As1e and N2 Primer Set Combo | || | Column | Definition | Possible Entries | | --- | --- | --- | | Sample # | Unique number for sample | Numeric (1-number of samples tested) | | Known Value | SAR-CoV-2 sample status | Positive, Negative | | Known Value | If Clinical sample what is the result from the clinical test; If contrived what amount of virus is used in units of copies per uL | Numeric | | Known Units | If Clinical sample what is the units from the clinical test; If contrived this must be copies/uL | Text (Ct, copies (of virus in reaction)) | | Result Value | Sample up to 1 decimal point | Numeric | | Result Units | Units used for reporting | Text (Ct, fluorescence) | | Result Interpretation | Based off of this result would you declare this positive or negative | Positive, Negative | || These are a few example runs, we have done over 100 runs in the last 2 months, 1000+ LAMP reactions. Many have been characterization and troubleshooting runs, as we've moved labs 3 times. We now have great exclusive use lab space at MBC Biolabs with: - A dedicated room with 2 biosafety cabinets where we will run the silica purification on inactivated samples - 4 lab benches where we'll install 2 PCR hood for setting up LAMP reactions - 1 5' chemical fume hood for preparing stock solutions involving acids and bases (Inactivation Solution, Binding Solution and Glass Milk) We have access to a GloMAX plate reader and ABI QuantStudio7 qPCR machine which we intend to use for comparison and characterization, and perhaps as part of our screening service offering. We have done many successful runs spiking Zeptometrix virions into raw samples at 1e4/ml level. The challenge is not getting good results on one run, but setting up a system to run continuously under control, especially eliminating failures due to contamination. We've moved to single use RNA controls, and aliquoting of many components in specific volumes in preparation for pilot runs.