<?xml version="1.0"?>
<rdf:RDF xmlns="http://purl.org/net/SMARTprotocol#"
xml:base="http://purl.org/net/SMARTprotocol"
xmlns:p1="http://purl.obolibrary.org/obo/iao/dev/ontology-metadata.owl#"
xmlns:p2="http://www.semanticweb.org/ontologies/2013/3/Ontology1365706525737.owl#"
xmlns:Ontology1184060740="http://www.owl-ontologies.com/Ontology1184060740.owl#"
xmlns:owl="http://www.w3.org/2002/07/owl#"
xmlns:owl2xml="http://www.w3.org/2006/12/owl2-xml#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#"
xmlns:swrlb="http://www.w3.org/2003/11/swrlb#"
xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#"
xmlns:xsp="http://www.owl-ontologies.com/2005/08/07/xsp.owl#"
xmlns:Thesaurus="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#"
xmlns:terms="http://purl.org/dc/terms/"
xmlns:xml="http://www.w3.org/XML/1998/namespace"
xmlns:iao="http://purl.obolibrary.org/obo/iao.owl#"
xmlns:vann="http://purl.org/vocab/vann/"
xmlns:SO="http://purl.org/obo/owl/SO#"
xmlns:sp="http://purl.org/net/SMARTprotocol#"
xmlns:MSH="http://purl.bioontology.org/ontology/MSH/"
xmlns:pav="http://purl.org/pav/"
xmlns:protege="http://protege.stanford.edu/plugins/owl/protege#"
xmlns:MGEDOntology="http://mged.sourceforge.net/ontologies/MGEDOntology.owl#"
xmlns:swrl="http://www.w3.org/2003/11/swrl#"
xmlns:bfo="http://www.ifomis.org/bfo/1.1#"
xmlns:miProtocol="http://www.semanticweb.org/ontologies/2013/3/miProtocol.owl#"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:ro="http://www.obofoundry.org/ro/ro.owl#"
xmlns:obo="http://purl.obolibrary.org/obo/"
xmlns:span="http://www.ifomis.org/bfo/1.1/span#"
xmlns:snap="http://www.ifomis.org/bfo/1.1/snap#">
<owl:Ontology rdf:about="http://purl.org/net/SMARTprotocol#">
<terms:contributor rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">http://www.alexandergarcia.name/</terms:contributor>
<vann:preferredNamespacePrefix rdf:datatype="http://www.w3.org/2001/XMLSchema#string">sp</vann:preferredNamespacePrefix>
<terms:creator rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">http://oxgiraldo.wordpress.com/</terms:creator>
<vann:preferredNamespaceUri rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://purl.org/net/SMARTprotocol#</vann:preferredNamespaceUri>
<rdfs:label xml:lang="en">SMART Protocols Ontology: Document Module</rdfs:label>
<terms:created rdf:datatype="http://www.w3.org/2001/XMLSchema#date">2013-07-01</terms:created>
<rdfs:comment xml:lang="en">SMART Protocols Ontology: Document Module is an ontology designed to represent metadata used to report an experimental protocol.</rdfs:comment>
<owl:versionInfo xml:lang="en">4.0</owl:versionInfo>
<terms:title xml:lang="en">SMART Protocols Ontology: Document Module</terms:title>
<terms:license>http://creativecommons.org/licenses/by/4.0/</terms:license>
<terms:contributor rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">http://www.dia.fi.upm.es/index.php?page=oscar-corcho/</terms:contributor>
<terms:description xml:lang="en">SMART Protocols Ontology: Document Module is an ontology designed to represent metadata used to report an experimental protocol.</terms:description>
</owl:Ontology>
<!--
///////////////////////////////////////////////////////////////////////////////////////
//
// Annotation properties
//
///////////////////////////////////////////////////////////////////////////////////////
-->
<!-- http://purl.obolibrary.org/obo/IAO_0000112 -->
<owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000112">
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A phrase describing how a class name should be used. May also include other kinds of examples that facilitate immediate understanding of a class semantics, such as widely known prototypical subclasses or instances of the class. Although essential for high level terms, examples for low level terms (e.g., Affymetrix HU133 array) are not</obo:IAO_0000115>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">example of usage</rdfs:label>
</owl:AnnotationProperty>
<!-- http://purl.obolibrary.org/obo/IAO_0000115 -->
<owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000115">
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">The official definition, explaining the meaning of a class or property. Shall be Aristotelian, formalized and normalized. Can be augmented with colloquial definitions.</obo:IAO_0000115>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">definition</rdfs:label>
</owl:AnnotationProperty>
<!-- http://purl.obolibrary.org/obo/IAO_0000118 -->
<owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000118">
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An alternative name for a class or property which means the same thing as the preferred name (semantically equivalent)</obo:IAO_0000115>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">alternative term</rdfs:label>
</owl:AnnotationProperty>
<!-- http://purl.obolibrary.org/obo/IAO_0000119 -->
<owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000119">
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">formal citation, e.g. identifier in external database to indicate / attribute source(s) for the definition. Free text indicate / attribute source(s) for the definition. EXAMPLE: Author Name, URI, MeSH Term C04, PUBMED ID, Wiki uri on 31.01.2007</obo:IAO_0000115>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">definition source</rdfs:label>
</owl:AnnotationProperty>
<!-- http://purl.obolibrary.org/obo/IAO_0000412 -->
<owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000412">
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">For external terms/classes, the ontology from which the term was imported</obo:IAO_0000115>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">imported from</rdfs:label>
</owl:AnnotationProperty>
<!-- http://purl.org/dc/terms/bibliographicCitation -->
<owl:AnnotationProperty rdf:about="http://purl.org/dc/terms/bibliographicCitation"/>
<!-- http://purl.org/dc/terms/description -->
<owl:AnnotationProperty rdf:about="http://purl.org/dc/terms/description"/>
<!--
///////////////////////////////////////////////////////////////////////////////////////
//
// Object Properties
//
///////////////////////////////////////////////////////////////////////////////////////
-->
<!-- http://purl.obolibrary.org/obo/BCGO_0000183 -->
<owl:ObjectProperty rdf:about="http://purl.obolibrary.org/obo/BCGO_0000183">
<rdfs:domain rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830"/>
<rdfs:domain rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<rdfs:domain rdf:resource="http://semanticscience.org/resource/SIO_001042"/>
<rdfs:range rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C41185"/>
<obo:IAO_0000115 xml:lang="en">The property has concentration relates a molecular entity, compound, substance, reagent or solute present in a unit amount of mixture.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has concentration</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.obolibrary.org/obo/OBI_0000304 -->
<owl:ObjectProperty rdf:about="http://purl.obolibrary.org/obo/OBI_0000304">
<obo:IAO_0000112 xml:lang="en">http://www.affymetrix.com/products/arrays/specific/hgu133.affx is_manufactered_by http://www.affymetrix.com/ (if we decide to use these URIs for the actual entities)</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">c is_manufactured_by o means that there was a process p in which c was built in which a person, or set of people or machines did the work(bore the &quot;Manufacturer Role&quot;, and those people/and or machines were members or of directed by the organization to do this.</obo:IAO_0000115>
<obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">has_manufacturer</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">is manufactured by</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.obolibrary.org/obo/RO_0001000 -->
<owl:ObjectProperty rdf:about="http://purl.obolibrary.org/obo/RO_0001000">
<obo:IAO_0000112 xml:lang="en">this cell derives from this parent cell (cell division); this nucleus derives from this parent nucleus (nuclear division).</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">derives from is a relation between two distinct material entities, the new entity and the old entity, in which the new entity begins to exist when the old entity ceases to exist, and the new entity inherits the significant portion of the matter of the old entity.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">derives_from</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">ro.owl</obo:IAO_0000412>
<rdfs:label xml:lang="en">derives from</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/OCRe/OCRe.owl#OCRE900225 -->
<owl:ObjectProperty rdf:about="http://purl.org/net/OCRe/OCRe.owl#OCRE900225">
<rdfs:subPropertyOf rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<rdfs:domain rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<obo:IAO_0000115 xml:lang="en">First name of a person</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">OCRe</obo:IAO_0000412>
<rdfs:label xml:lang="en">has first name</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/OCRe/OCRe.owl#OCRE900226 -->
<owl:ObjectProperty rdf:about="http://purl.org/net/OCRe/OCRe.owl#OCRE900226">
<rdfs:subPropertyOf rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<rdfs:domain rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<obo:IAO_0000115 xml:lang="en">Last name of a person</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">OCRe</obo:IAO_0000412>
<rdfs:label xml:lang="en">has last name</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#acceptedBy -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#acceptedBy">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#Publication"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#Repository"/>
<obo:IAO_0000115 xml:lang="en">The property acceptedBy indicates the person or authoritative body who, after theprocess of validation of a protocol, accepts the modifications made to this document before to its massive implementation.</obo:IAO_0000115>
<rdfs:label xml:lang="en">accepted by</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#adaptedFrom -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#adaptedFrom">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol"/>
<obo:IAO_0000115 xml:lang="en">the property adaptedFrom is the retationship that binds a protocol to a provenance of the protocol</obo:IAO_0000115>
<rdfs:label xml:lang="en">adapted from</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasAdvantage -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasAdvantage">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#AdvantageOfTheProtocol"/>
<obo:IAO_0000115 xml:lang="en">hasAdvantage is a relation that binds an experimental protocol to an advantage of the protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has advantage</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasAlertMessage -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasAlertMessage">
<rdfs:range rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">"DNA Extraction Protocol" hasAlertMessage "Critical Step"</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">hasAlertMessage is a relation that binds a chemical substance or protocol to an alert message such as caution, critical step, timing, etc.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has alert message</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasAnnealingTemperature -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasAnnealingTemperature">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">The property hasAnnealingTemperature indicates the temperature allowing annealing of the primers to the single-stranded DNA template during a PCR. The temperature should be from 42-65°C.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">has melting temperature</obo:IAO_0000118>
<rdfs:label xml:lang="en">has annealing temperature</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasAnnealingTime -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasAnnealingTime">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">The property hasAnnealingTime indicates the time allowing annealing of the primers to the single-stranded DNA template during a PCR. Typical annealing times are 15-30 seconds.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">has melting time</obo:IAO_0000118>
<rdfs:label xml:lang="en">has annealing time</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasApplication -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasApplication">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#ApplicationOfTheProtocol"/>
<obo:IAO_0000115 xml:lang="en">hasApplication is a relation that binds a document such as experimental protocol to a application of the protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has application</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasAuthor -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasAuthor">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">"Laboratory protocol" hasAuthor "John Smith"</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">hasAuthor is a relation that binds a document such as experimental protocol to an author</obo:IAO_0000115>
<rdfs:label xml:lang="en">has author</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasAuthorIdentification -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasAuthorIdentification">
<rdfs:domain rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#AuthorIdentifier"/>
<obo:IAO_0000115 xml:lang="en">hasAuthorIdentification is a relation that binds an author to an author identifier.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has author identification</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasCatalogNumber -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasCatalogNumber">
<rdfs:domain rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#CatalogNumber"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">"Nalgene Cryogenic Tube" hasCatalogNumber "5000-0012"</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">hasCatalogNumber is a relation that binds a material entity to a numeral or string of numerals that is used for identification.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has catalog number</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasDevelopmentalStage -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasDevelopmentalStage">
<obo:IAO_0000115 xml:lang="en">hasDevelopmentalStage is a relation that binds a specimen to an developmental stage.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has developmental stage</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasExperimentalInputs -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasExperimentalInputs">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">hasExperimentalInput is a relation that binds a experimental protocol to a material entity participating in a protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has experimental input</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasFinalConcentration -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasFinalConcentration">
<rdfs:subPropertyOf rdf:resource="http://purl.obolibrary.org/obo/BCGO_0000183"/>
<rdfs:domain rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#FinalConcentration"/>
<obo:IAO_0000115 xml:lang="en">The property hasFinalConcentration relates the final measured concentration of a molecular entity, compound, substance, reagent or solute and the class final concentration.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has final concentration</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasGrowthConditions -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasGrowthConditions">
<obo:IAO_0000115 xml:lang="en">hasGrowthConditions is a relation that binds a specimen entry to an growth conditions entry</obo:IAO_0000115>
<rdfs:label xml:lang="en">has growth conditions</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasIdentifier -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasIdentifier">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolIdentifier"/>
<obo:IAO_0000115 xml:lang="en">hasIdentifier is a relation that binds a document such as an experimental protocol to an protocol identifier</obo:IAO_0000115>
<rdfs:label xml:lang="en">has identifier</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasInitialConcentration -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasInitialConcentration">
<rdfs:subPropertyOf rdf:resource="http://purl.obolibrary.org/obo/BCGO_0000183"/>
<rdfs:domain rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<rdfs:range rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C85644"/>
<obo:IAO_0000115 xml:lang="en">The property hasInitialConcentration relates the first measured concentration of a molecular entity, compound, substance, reagent or solute and the class initial concentration.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has initial concentration</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasInstruction -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasInstruction">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolInstruction"/>
<obo:IAO_0000115 xml:lang="en">hasInstruction is a relation that binds a experimental protocol to a protocol instruction.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has instruction</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasLimitation -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasLimitation">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#LimitationOfTheProtocol"/>
<obo:IAO_0000115 xml:lang="en">hasLimitation is a relation that binds a document such as experimental protocol to a limitation of protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has limitation</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasName -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasName">
<rdfs:domain rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<rdfs:range rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">"Buffer" hasName "1X Queen’s lysis buffer (1L)"</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">hasName binds to a person, animal, place or thing to a word or set of words by which someone or something is known, addressed, or referred to.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has name</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasOutcome -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasOutcome">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#OutcomeOfTheProtocol"/>
<obo:IAO_0000115 xml:lang="en">hasOutcome is a relation that binds a document such as experimental protocol to an outcome of the protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has outcome</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasPrimerLength -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasPrimerLength">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">The property primerLength indicates the length of PCR primers. This length is long enough for adequate specificity and short enough for primers to bind easily to the template at the annealing temperature. The typical length of PCR primers is 18-30bp.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has primer length</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasPurpose -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasPurpose">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#PurposeOfProtocol"/>
<obo:IAO_0000115 xml:lang="en">hasPurpose is a relation that binds a document such as experimental protocol to an purpose of the protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has purpose</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasQuantity -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasQuantity">
<obo:IAO_0000115 xml:lang="en">The property hasQuantity indicates how much there is of something that you can measure; the total amount or number.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has quantity</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasSequence -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasSequence">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#PrimerSequence"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">The primer CII-fw hasSequence 5′-GAATGATGTACCACCTTTG-3′</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">hasSequence is a relation that binds a oligonucleotide or nucleic acid to a sequence.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has sequence</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasSpecimenTreatment -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasSpecimenTreatment">
<obo:IAO_0000115 xml:lang="en">hasSpecimenTreatment is a relation that binds a specimen to a specimen treatment protocol</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">has sample treatment</obo:IAO_0000118>
<rdfs:label xml:lang="en">has specimen treatment</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasSubject -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasSubject">
<obo:IAO_0000115 xml:lang="en">The property hasSubject indicates the topic or topics of a resource, for example a protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has subject</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasTechnique -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasTechnique">
<rdfs:label xml:lang="en">has technique</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasTitle -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#hasTitle">
<owl:inverseOf rdf:resource="http://purl.org/net/SMARTprotocol#titleOf"/>
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">"Laboratory protocol" hasTitle "An Optimized Chloroplast DNA Extraction Protocol for Grasses (Poaceae) Proves Suitable for Whole Plastid Genome Sequencing and SNP Detection"</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">hasTitle is a relation that binds a document such as laboratory protocol to a title of protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">has title</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#locatedIn -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#locatedIn">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#Repository"/>
<obo:IAO_0000115 xml:lang="en">the property publishedIn is the retationship that binds a Protocol to a RepositoryName</obo:IAO_0000115>
<rdfs:label xml:lang="en">located in</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#modifiedBy -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#modifiedBy">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">The property modifiedBy indicates the person or authoritative body who changed an item, for example, a protocol.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">edited by</obo:IAO_0000118>
<rdfs:label xml:lang="en">modified by</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#publishedIn -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#publishedIn">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.org/net/SMARTprotocol#Publication"/>
<obo:IAO_0000115 xml:lang="en">the property publishedIn is the retationship that binds a protocol to a publicationName</obo:IAO_0000115>
<rdfs:label xml:lang="en">published in</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#titleOf -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#titleOf">
<rdf:type rdf:resource="http://www.w3.org/2002/07/owl#TransitiveProperty"/>
<rdfs:label xml:lang="en">title of</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#validatedBy -->
<owl:ObjectProperty rdf:about="http://purl.org/net/SMARTprotocol#validatedBy">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">The property validatedBy indicates the person who formally assesses or examines that the protocol can be understood and carried out correctly by users.</obo:IAO_0000115>
<rdfs:label xml:lang="en">validated by</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/pav/hasCurrentVersion -->
<owl:ObjectProperty rdf:about="http://purl.org/pav/hasCurrentVersion">
<rdfs:range rdf:resource="http://purl.obolibrary.org/obo/IAO_0000129"/>
<obo:IAO_0000412 xml:lang="en">PAV ontology</obo:IAO_0000412>
<rdfs:label xml:lang="en">has current version</rdfs:label>
</owl:ObjectProperty>
<!-- http://purl.org/pav/hasVersion -->
<owl:ObjectProperty rdf:about="http://purl.org/pav/hasVersion">
<rdfs:domain rdf:resource="http://purl.obolibrary.org/obo/ERO_0000071"/>
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.obolibrary.org/obo/IAO_0000129"/>
<obo:IAO_0000412 xml:lang="en">PAV ontology</obo:IAO_0000412>
<rdfs:label xml:lang="en">has version</rdfs:label>
</owl:ObjectProperty>
<!-- http://semanticscience.org/resource/SIO_000364 -->
<owl:ObjectProperty rdf:about="http://semanticscience.org/resource/SIO_000364">
<rdfs:domain rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<rdfs:range rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<obo:IAO_0000112 xml:lang="en">the experimental protocol A was created by B author.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">has creator is a relation between an entity, such as, an author and the protocol that this person have created.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">was created by
is created by</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">Semanticscience Integrated Ontology</obo:IAO_0000412>
<rdfs:label xml:lang="en">has creator</rdfs:label>
</owl:ObjectProperty>
<!-- http://www.obofoundry.org/ro/ro.owl#has_part -->
<owl:ObjectProperty rdf:about="http://www.obofoundry.org/ro/ro.owl#has_part">
<owl:inverseOf rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<rdf:type rdf:resource="http://www.w3.org/2002/07/owl#TransitiveProperty"/>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">has part</rdfs:label>
</owl:ObjectProperty>
<!-- http://www.obofoundry.org/ro/ro.owl#part_of -->
<owl:ObjectProperty rdf:about="http://www.obofoundry.org/ro/ro.owl#part_of">
<rdf:type rdf:resource="http://www.w3.org/2002/07/owl#TransitiveProperty"/>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Parthood as a relation between instances: The primitive instance-level relation p part_of p1 is illustrated in assertions such as: this instance of rhodopsin mediated phototransduction part_of this instance of visual perception. This relation satisfies at least the following standard axioms of mereology: reflexivity (for all p, p part_of p); anti-symmetry (for all p, p1, if p part_of p1 and p1 part_of p then p and p1 are identical); and transitivity (for all p, p1, p2, if p part_of p1 and p1 part_of p2, then p part_of p2). Analogous axioms hold also for parthood as a relation between spatial regions. For parthood as a relation between continuants, these axioms need to be modified to take account of the incorporation of a temporal argument. Thus for example the axiom of transitivity for continuants will assert that if c part_of c1 at t and c1 part_of c2 at t, then also c part_of c2 at t. Parthood as a relation between classes: To define part_of as a relation between classes we again need to distinguish the two cases of continuants and processes, even though the explicit reference to instants of time now falls away. For continuants, we have C part_of C1 if and only if any instance of C at any time is an instance-level part of some instance of C1 at that time, as for example in: cell nucleus part_ of cell.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBO Relation Ontology</obo:IAO_0000412>
<rdfs:label xml:lang="en">part of</rdfs:label>
</owl:ObjectProperty>
<!--
///////////////////////////////////////////////////////////////////////////////////////
//
// Classes
//
///////////////////////////////////////////////////////////////////////////////////////
-->
<!-- http://mged.sourceforge.net/ontologies/MGEDOntology.owl#Cultivar -->
<owl:Class rdf:about="http://mged.sourceforge.net/ontologies/MGEDOntology.owl#Cultivar">
<rdfs:subClassOf rdf:resource="http://mged.sourceforge.net/ontologies/MGEDOntology.owl#StrainOrLine"/>
<obo:IAO_0000115 xml:lang="en">a plant variety obtained in agriculture in horticulture.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Microarray and Gene Expression Data Ontology (MGEDO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">cultivar</rdfs:label>
</owl:Class>
<!-- http://mged.sourceforge.net/ontologies/MGEDOntology.owl#Ecotype -->
<owl:Class rdf:about="http://mged.sourceforge.net/ontologies/MGEDOntology.owl#Ecotype">
<rdfs:subClassOf rdf:resource="http://mged.sourceforge.net/ontologies/MGEDOntology.owl#StrainOrLine"/>
<obo:IAO_0000115 xml:lang="en">a biotype resulting from selection in a particular habitat, e.g. the A. thaliana Ecotype Ler</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Microarray and Gene Expression Data Ontology (MGEDO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">ecotype</rdfs:label>
</owl:Class>
<!-- http://mged.sourceforge.net/ontologies/MGEDOntology.owl#StrainOrLine -->
<owl:Class rdf:about="http://mged.sourceforge.net/ontologies/MGEDOntology.owl#StrainOrLine">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
<obo:IAO_0000115 xml:lang="en">A strain or line is an animal or plant offspring that has a single ancestral breeding pair or parent as a result of brother x sister or parent x offspring matings. This class is extended to include F1 offspring and established breeding lines. For microbes, these are isolates derived from nature or in the laboratory.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">organism strain
organism line</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Microarray and Gene Expression Data Ontology (MGEDO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">strain or line</rdfs:label>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C12801 -->
<owl:Class rdf:about="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C12801">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0000671"/>
<obo:IAO_0000115 xml:lang="en">An anatomical structure consisting of similarly specialized cells and intercellular matrix, aggregated according to genetically determined spatial relationships, performing a specific function.</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CDISC</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">NCI thesaurus</obo:IAO_0000412>
<rdfs:label xml:lang="en">tissue</rdfs:label>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C41185 -->
<owl:Class rdf:about="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C41185">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#Quality"/>
<obo:IAO_0000115 xml:lang="en">The quantity of a substance per unit volume or weight; a measure of the amount of substance present in a unit amount of mixture, particularly, the amount of solute dissolved in a solvent. The amounts can be expressed as moles, masses, volumes, or parts.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">NCI thesaurus</obo:IAO_0000412>
<rdfs:label xml:lang="en">concentration</rdfs:label>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614 -->
<owl:Class rdf:about="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ApplicationOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#CatalogNumber"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#LimitationOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PrimerSequence"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PurposeOfProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<owl:disjointWith rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">The words or language units by which a thing is known.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">NCI thesaurus</obo:IAO_0000412>
<rdfs:label xml:lang="en">name</rdfs:label>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42688 -->
<owl:Class rdf:about="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42688">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<obo:IAO_0000115 xml:lang="en">A message describing how something is to be done.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">step</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">NCIT</obo:IAO_0000412>
<rdfs:label xml:lang="en">Instruction</rdfs:label>
<rdfs:label xml:lang="en">instruction</rdfs:label>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218 -->
<owl:Class rdf:about="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#EthanolSolution"/>
<obo:IAO_0000115 xml:lang="en">An aqueous dilution of ethanol containing 70% ethanol by volume.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">70% Ethyl Alcohol
Ethyl Alcohol, 70%
Alcohol, Ethyl 70%
70% (v/v) ethanol
70 per cent alcohol
70% RNase-free ethanol
70% RNase free ethanol
70% EtOH</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">NCIThesaurus</obo:IAO_0000412>
<rdfs:label xml:lang="en">70% Ethanol</rdfs:label>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830 -->
<owl:Class rdf:about="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.obolibrary.org/obo/BCGO_0000183"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#FinalConcentration"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAlertMessage"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#StorageCondition"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAlertMessage"/>
<owl:someValuesFrom rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SolutionName"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://semanticscience.org/resource/SIO_000070"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33695"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/OBI_0000747"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#Manufacturer"/>
<obo:IAO_0000115 xml:lang="en">A homogeneous mixture of two or more substances; frequently (but not necessarily) a liquid solution.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">NCI thesaurus</obo:IAO_0000412>
<rdfs:label xml:lang="en">solution</rdfs:label>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C85644 -->
<owl:Class rdf:about="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C85644">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C41185"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#FinalConcentration"/>
<obo:IAO_0000115 xml:lang="en">The first measured concentration of a compound in a substance. In pharmacology, the initial concentration is given only for bolus IV models.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">NCI thesaurus</obo:IAO_0000412>
<rdfs:label xml:lang="en">initial concentration</rdfs:label>
</owl:Class>
<!-- http://prismstandard.org/namespaces/basic/2.0/prism:coverDate -->
<owl:Class rdf:about="http://prismstandard.org/namespaces/basic/2.0/prism:coverDate">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<obo:IAO_0000115 xml:lang="en">Date on the cover of a magazine issue, suitable for storing in a database field with a 'date' data type.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">PRISM</obo:IAO_0000412>
<rdfs:comment xml:lang="en">The cover date on a magazine indicates the last date the printed issue should be displayed for sale, NOT the publication date as commonly believed.
Not applicable to non-print platforms.</rdfs:comment>
<rdfs:label xml:lang="en">cover date</rdfs:label>
</owl:Class>
<!-- http://prismstandard.org/namespaces/basic/2.0/prism:keyword -->
<owl:Class rdf:about="http://prismstandard.org/namespaces/basic/2.0/prism:keyword">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<obo:IAO_0000115 xml:lang="en">An element used to tag keywords that are likely to be used in search queries.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">PRISM</obo:IAO_0000412>
<rdfs:comment xml:lang="en">Note that this differs from a subject or elements such as dc:subject, prism:person, prism:event, or prism:organization that are the subject of the article. Best practice is to use values from a keyword controlled vocabulary for this element</rdfs:comment>
<rdfs:label xml:lang="en">keyword</rdfs:label>
</owl:Class>
<!-- http://prismstandard.org/namespaces/basic/2.0/prism:publicationName -->
<owl:Class rdf:about="http://prismstandard.org/namespaces/basic/2.0/prism:publicationName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<obo:IAO_0000115 xml:lang="en">Title of the magazine, or other publication, in which a resource was/will be published.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">repository name</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">PRISM</obo:IAO_0000412>
<rdfs:label xml:lang="en">publication name</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/CSP/4000-0119 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/CSP/4000-0119">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C12801"/>
<obo:IAO_0000115 xml:lang="en">tissue and cells derived from animals for use in research.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">nonhuman tissue</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CRISP</obo:IAO_0000412>
<rdfs:label xml:lang="en">animal tissue</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/CSP/4001-0092 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/CSP/4001-0092">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/BTO_0004725"/>
<obo:IAO_0000115>a ubiquitous tissue culture model for nontransformed animal cells; derived from nonneoplastic immortalized fibroblasts.</obo:IAO_0000115>
<obo:IAO_0000412>CRISP</obo:IAO_0000412>
<rdfs:label>3T3 cell</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/MSH/D004864 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/MSH/D004864">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/OBI_0000747"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">Expendable and nonexpendable equipment, supplies, apparatus, and instruments that are used in diagnostic, surgical, therapeutic, scientific, and experimental procedures.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Device
Apparatus and Instruments
Device, Medical
Devices, Medical
Instruments and Apparatus
Inventory
Medical Device
Supplies and Equipment
MED DEVICE
EQUIPMENT SUPPLIES
MED DEVICES
APPARATUS INSTRUM
DEVICES MED
DEVICE MED
Devices
Equipment
Inventories
Medical Devices
Supplies</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Medical Subject Headings (MeSH)</obo:IAO_0000412>
<rdfs:label xml:lang="en">equipment and supplies</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/NCBITAXON/39700 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/NCBITAXON/39700">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/NCBITAXON/5690"/>
<obo:IAO_0000412 xml:lang="en">NCBITaxon</obo:IAO_0000412>
<rdfs:label xml:lang="en">Trypanozoon</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/NCBITAXON/47569 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/NCBITAXON/47569">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/NCBITAXON/5690"/>
<obo:IAO_0000412 xml:lang="en">NCBITaxon</obo:IAO_0000412>
<rdfs:label xml:lang="en">Nannomonas</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/NCBITAXON/5690 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/NCBITAXON/5690">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
<obo:IAO_0000115 xml:lang="en">Trypanosoma is a genus of kinetoplastids (class Kinetoplastida), a monophyletic group of unicellular parasitic flagellate protozoa.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Trypanosoma</obo:IAO_0000119>
<obo:IAO_0000412 xml:lang="en">NCBITaxon</obo:IAO_0000412>
<rdfs:label xml:lang="en">Trypanosoma</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/NCBITAXON/5691 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/NCBITAXON/5691">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/NCBITAXON/39700"/>
<obo:IAO_0000118 xml:lang="en">Trypanosoma (Trypanozoon) brucei
Trypanosoma brucei subgroup</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">NCBITaxon</obo:IAO_0000412>
<rdfs:label xml:lang="en">Trypanosoma brucei</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/NCBITAXON/5692 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/NCBITAXON/5692">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/NCBITAXON/47569"/>
<obo:IAO_0000118 xml:lang="en">T. congolense</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">NCBITaxon</obo:IAO_0000412>
<rdfs:label xml:lang="en">Trypanosoma congolense</rdfs:label>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/SNOMEDCT/285297004 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/SNOMEDCT/285297004">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000000"/>
<obo:IAO_0000412 xml:lang="en">SNOMEDCT</obo:IAO_0000412>
<rdfs:label xml:lang="en">bacterial cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/BFO_0000023 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/BFO_0000023">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#SpecificallyDependentContinuant"/>
<obo:IAO_0000115 xml:lang="en">A realizable entity the manifestation of which brings about some result or end that is not essential to a continuant in virtue of the kind of thing that it is but that can be served or participated in by that kind of continuant in some kinds of natural, social or institutional contexts.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">BFO</obo:IAO_0000412>
<rdfs:label xml:lang="en">role</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/BTO_0000007 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/BTO_0000007">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/BTO_0002733"/>
<obo:IAO_0000115 xml:lang="en">Established from a human primary embryonal kidney transformed by adenovirus type 5.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">A293 cell
HEK cell
293 cell
A-293 cell
HEK293 cell
human embryonic kidney cell
human embryonal kidney cell</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">HEK-293 cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/BTO_0000669 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/BTO_0000669">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000548"/>
<obo:IAO_0000412 xml:lang="en">Brenda Tissue Ontology</obo:IAO_0000412>
<rdfs:label xml:lang="en">embryonic cell line</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/BTO_0000944 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/BTO_0000944">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4001-0092"/>
<obo:IAO_0000115>Very widely used mouse fibroblast cell line; 3T3 cells have been derived from different mouse strains and it is therefore important to define the particular cell line. NIH strain were from the National Institute of Health in the USA; Mouse embryonic fibroblast cell line, established from disaggregated Swiss albino mouse embryos in 1962.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">3T3-Swiss albino cell
NIH 3T3 cell
3T3 cell
NIH3T3 cell
3T3 fibroblast
NIH/3T3 cell</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">Brenda Tissue Ontology</obo:IAO_0000412>
<rdfs:label>NIH-3T3 cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/BTO_0002733 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/BTO_0002733">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/BTO_0000669"/>
<obo:IAO_0000412 xml:lang="en">Brenda Tissue Ontology</obo:IAO_0000412>
<rdfs:label xml:lang="en">embryonic kidney cell line</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/BTO_0004725 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/BTO_0004725">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000057"/>
<obo:IAO_0000412 xml:lang="en">Brenda Tissue Ontology</obo:IAO_0000412>
<rdfs:label xml:lang="en">embryonic fibroblast</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_1418 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_1418">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#AlkanesulfonateReagent"/>
<obo:IAO_0000115 xml:lang="en">an alkanesulfonate that has formula C32H58N2O7S.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">C32H58N2O7S
3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">CHAPS</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_15693 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_15693">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_35381"/>
<obo:IAO_0000115 xml:lang="en">Aldehydic parent sugars (polyhydroxy aldehydes H[CH(OH)]nC(=O)H, n >= 2) and their intramolecular hemiacetals.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">C2H4O2(CH2O)n
an aldose
aldoses
Aldose</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">aldose</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_15734 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_15734">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_30879"/>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">primary alcohol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_15882 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_15882">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33853"/>
<obo:IAO_0000115 xml:lang="en">An organic hydroxy compound that consists of benzene bearing a single hydroxy substituent. The parent of the class of phenols.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Hydroxybenzene
carbolic acid
acide phenique
C6H6O
Phenic acid
Phenylic acid
acide carbolique
Benzenol
Phenylic alcohol
PhOH
Carbolsaeure
Oxybenzene
Karbolsaeure</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">phenol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_16236 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_16236">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_15734"/>
<obo:IAO_0000115 xml:lang="en">A primary alcohol that is ethane in which one of the hydrogens is substituted by a hydroxy group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">OXYDIMETHYLENE GROUP
Aethylalkohol
Ethyl alcohol
Aethanol
spiritus vini
hydroxyethane
EtOH
1-hydroxyethane
etanol
Methylcarbinol
alcool ethylique
alcohol etilico
alcohol
Dehydrated ethanol
C2H6O
[OEtH]
[CH2Me(OH)]
CCO
C2H5OH
Alkohol</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">ethanol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_16325 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_16325">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#BileAcidReagent"/>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A monohydroxy-5beta-cholanic acid with a alpha-hydroxy substituent at position 3. It is a bile acid obtained from chenodeoxycholic acid by bacterial action.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">3c-5beta-cholan-24-oic acid
Lithocholic acid
C24H40O3
3alpha-Hydroxy-5beta-cholanic acid
(3alpha,5beta)-3-hydroxycholan-24-oic acid
3alpha-hydroxy-5beta-cholanoic acid
3alpha-hydroxy-5beta-cholanic acid
3alpha-Hydroxy-5beta-cholanate
5beta-cholanic acid-3alpha-ol</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">lithocholic acid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_16359 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_16359">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#BileAcidReagent"/>
<obo:IAO_0000112 xml:lang="en">Non-denaturing ionic detergent used for extraction of membrane proteins.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A bile acid that is 5beta-cholan-24-oic acid bearing three alpha-hydroxy substituents at position 3, 7 and 12.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">CHOLIC ACID
Cholic acid
3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid
3alpha,7alpha,12alpha-Trihydroxy-5beta-cholanic acid
Cholsaeure
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanic acid
(3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxycholan-24-oic acid
C24H40O5
3alpha,7alpha,12alpha-Trihydroxy-5beta-cholanate</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">cholic acid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_16526 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_16526">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_64708"/>
<obo:IAO_0000115 xml:lang="en">A one-carbon compound with formula CO2 in which the carbon is attached to each oxygen atom by a double bond. A colourless, odourless gas under normal conditions, it is produced during respiration by all animals, fungi and microorganisms that depend directly or indirectly on living or decaying plants for food.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">CARBON DIOXIDE
dioxidocarbon
carbon dioxide
Carbon dioxide
CO2
E-290
E290
E 290
dioxomethane
carbonic anhydride
O=C=O
R-744</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">carbon dioxide</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_16646 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_16646">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">Any member of the class of organooxygen compounds that is a polyhydroxy-aldehyde or -ketone or a lactol resulting from their intramolecular condensation (monosaccharides); substances derived from these by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom; and polymeric products arising by intermolecular acetal formation between two or more such molecules (disaccharides, polysaccharides and oligosaccharides). Carbohydrates contain only carbon, hydrogen and oxygen atoms; prior to any oxidation or reduction, most have the empirical formula Cm(H2O)n. Compounds obtained from carbohydrates by substitution, etc., are known as carbohydrate derivatives and may contain other elements. Cyclitols are generally not regarded as carbohydrates.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Kohlenhydrat
glucido
hydrates de carbone
glucides
carbohydrates
Kohlenhydrate
saccharidum
glucidos
carbohidratos
saccharide
carbohidrato
saccharides
glucide
carbohydrate</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">carbohydrate</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_16755 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_16755">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#BileAcidReagent"/>
<obo:IAO_0000112 xml:lang="en">chenodeoxycholic acid acts as an anionic detergents that often completely disrupt cell membranes and fully denature proteins.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A dihydroxy-5beta-cholanic acid that is (5beta)-cholan-24-oic acid substituted by hydroxy groups at positions 3 and 7 respectively.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid
Chenodeoxycholic acid
Chenodiol
gallodesoxycholic acid
anthropodeoxycholic acid
CDCA
chenic acid
3alpha,7alpha-Dihydroxy-5beta-cholanic acid
Chenix
C24H40O4
anthropodesoxycholic acid
7alpha-hydroxylithocholic acid</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">chenodeoxycholic acid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_16991 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_16991">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33696"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">High molecular weight, linear polymers, composed of nucleotides containing deoxyribose and linked by phosphodiester bonds; DNA contain the genetic information of organisms.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">ChEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">deoxyribonucleic acid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_17234 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_17234">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33917"/>
<obo:IAO_0000115 xml:lang="en">An aldohexose used as a source of energy and metabolic intermediate.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">glucose
C6H12O6
Glc
Glukose
Glucose
gluco-hexose</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">glucose</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_17790 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_17790">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_15734"/>
<obo:IAO_0000115>The primary alcohol that is the simplest aliphatic alcohol, comprising a methyl and an alcohol group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Methylalkohol
wood alcohol
CH3OH
Methyl alcohol
wood naphtha
carbinol
MeOH
wood spirit
OXYMETHYLENE BRIDGING GROUP
CH4O
CO</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label>methanol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_17824 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_17824">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_35681"/>
<obo:IAO_0000115 xml:lang="en">A secondary alcohol that is propane in which one of the hydrogens attached to the central carbon is substituted by a hydroxy group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">i-propanol
C3H8O
Isopropanol
1-methylethyl alcohol
IPA
sec-propanol
i-Propylalkohol
Isopropyl alcohol
2-Propanol
isopropyl alcohol
CC(C)O
Isopropylalkohol
2-hydroxypropane
ISOPROPYL ALCOHOL
1-methylethanol</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">propan-2-ol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_17843 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_17843">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">A single-stranded RNA molecule containing about 70-90 nucleotides, folded by intrastrand base pairing into a characteristic secondary ('cloverleaf') structure that carries a specific amino acid and matches it to its corresponding codon on an mRNA during protein synthesis.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">tRNA;
RNA, transfer</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">transfer RNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_17992 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_17992">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#DisaccharideReagent"/>
<obo:IAO_0000115 xml:lang="en">Sucrose is a disaccharide formed by glucose and fructose units joined by an acetal oxygen bridge from hemiacetal of glucose to the hemiketal of the fructose.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Sucrose
beta-D-fructofuranosyl alpha-D-glucopyranoside
SUCROSE
sacarosa
beta-D-Fruf-(21)-alpha-D-Glcp
Saccharose
table sugar
1-alpha-D-Glucopyranosyl-2-beta-D-fructofuranoside
Sacharose
Cane sugar
White sugar</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">sucrose</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_18111 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_18111">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">RNA molecules which are essential structural and functional components of ribosomes, the subcellular units responsible for protein synthesis.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">rRNA</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">ribosomal RNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_24663 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_24663">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_36278"/>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">hydroxy-5beta-cholanic acid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_24840 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_24840">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SulfateSaltReagent"/>
<obo:IAO_0000118 xml:lang="en">inorganic sulfate salts
inorganic sulfates</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">inorganic sulfate salt</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_2651 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_2651">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">Any ribonucleic acid that is transfer RNA in which one or more of the free OH groups carries an alpha-aminoacyl group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Aminoacyl-tRNA</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">alpha-aminoacyl-tRNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_26710 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_26710">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_36093"/>
<obo:IAO_0000115 xml:lang="en">An inorganic chloride salt having sodium(1+) as the counterion.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Natriumchlorid
halite
[Na+].[Cl-]
common salt
Kochsalz
NaCl
ClNa
salt
cloruro sodico
rock salt
chlorure de sodium
natrii chloridum
table salt</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">sodium chloride</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_27729 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_27729">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SaponinReagent"/>
<obo:IAO_0000112 xml:lang="en">digitonin acts as a nonionic detergent used to solubilize receptors and permeabilize cellular and nuclear membranes.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A spirostanyl glycoside that has formula C56H92O29.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Digitin
digitin
Digitonin</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">digitonin</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_28834 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_28834">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#BileAcidReagent"/>
<obo:IAO_0000115 xml:lang="en">A dihydroxy-5beta-cholanic acid that has formula C24H40O4.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Deoxycholic acid
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid (3alpha,5alpha,8alpha,12alpha,14beta,17alpha)-3,12-dihydroxycholan-24-oic acid desoxycholic acid
Desoxycholsaeure
C24H40O4
7alpha-deoxycholic acid
(3alpha,5beta,12alpha)-3,12-dihydroxycholan-24-oic acid (3ALPHA,5ALPHA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC ACID 3alpha,12alpha-Dihydroxy-5beta-cholanic acid</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">deoxycholic acid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_30879 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_30879">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">A compound in which a hydroxy group, -OH, is attached to a saturated carbon atom.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">O[*]
an alcohol
HOR</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">alcohol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_32029 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_32029">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PotassiumSaltReagent"/>
<obo:IAO_0000112 xml:lang="en">In molecular biology, potassium acetate is used to precipitate dodecyl sulfate (DS) and DS-bound proteins, allowing the removal of proteins from DNA. It is also used as a salt for the ethanol precipitation of DNA.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A potassium salt that has formula C2H3O2.K.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Potassium acetate
Potassium Acetate</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">potassium acetate</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_32588 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_32588">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PotassiumSaltReagent"/>
<obo:IAO_0000115 xml:lang="en">potassium chloride (KCl) is a inorganic chloride composed of potassium and chloride.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">ClK
potassium chloride
sylvite
KCl
Kaon-Cl 10
Kaliumchlorid
muriate of potash
[KCl]
Klotrix
Klor-con
Monopotassium chloride</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Potassium_chloride</obo:IAO_0000119>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">potassium chloride</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_32954 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_32954">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_38700"/>
<obo:IAO_0000115 xml:lang="en">An organic sodium salt that has formula C2H3NaO2.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sodium acetate anhydrous
C2H3NaO2
anhydrous sodium acetate
acetic acid, sodium salt
[Na+].CC([O-])=O
Natriumazetat</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">sodium acetate</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33659 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_33659">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000118 xml:lang="en">organic aromatic compounds</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">organic aromatic compound</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33695 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_33695">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0000671"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">ChEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">information biomacromolecule</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33696 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_33696">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33695"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/CHEBI_36080"/>
<obo:IAO_0000115 xml:lang="en">A macromolecule made up of nucleotide units and hydrolysable into certain pyrimidine or purine bases (usually adenine, cytosine, guanine, thymine, uracil), D-ribose or 2-deoxy-D-ribose and phosphoric acid.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">ChEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">nucleic acid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33697 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_33697">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33696"/>
<obo:IAO_0000115 xml:lang="en">High molecular weight, linear polymers, composed of nucleotides containing ribose and linked by phosphodiester bonds; RNA is central to the synthesis of proteins.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">ChEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">ribonucleic acid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33699 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_33699">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">An RNA molecule that transfers the coding information for protein synthesis from the chromosomes to the ribosomes mRNA is formed from a DNA template by transcription. It may be a copy of a single gene or of several adjacent genes (polycistronic mRNA). On the ribosome, the sequence is converted into the programmed amino acid sequence through translation.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">template RNA;
mRNA</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">messenger RNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33853 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_33853">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33659"/>
<obo:IAO_0000115 xml:lang="en">Organic aromatic compounds having one or more hydroxy groups attached to a benzene or other arene ring.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">aryl alcohol
arenols
Aryl alcohol</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">phenols</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33893 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_33893">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.obolibrary.org/obo/OBI_0000304"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ManufacturerName"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAlertMessage"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#StorageCondition"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAlertMessage"/>
<owl:someValuesFrom rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasCatalogNumber"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#CatalogNumber"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ReagentName"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://semanticscience.org/resource/SIO_000070"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://semanticscience.org/resource/SIO_001042"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/ERO_0000071"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/OBI_0000747"/>
<owl:disjointWith rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#Kit"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#Manufacturer"/>
<obo:IAO_0000115 xml:lang="en">A substance used in a chemical reaction to detect, measure, examine, or produce other substances.</obo:IAO_0000115>
<obo:IAO_0000118>chemical compound</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">ChEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">reagent</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33917 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_33917">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_15693"/>
<obo:IAO_0000115 xml:lang="en">A hexose with a (potential) aldehyde group at one end.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">aldohexoses
Aldohexose
OCC(O)C(O)C(O)C(O)C=O
aldohexose
C6H12O6</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">aldohexose</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_35255 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_35255">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_64708"/>
<obo:IAO_0000115 xml:lang="en">A one-carbon compound that is methane in which three of the hydrogens are replaced by chlorines.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">trichloromethane
CHCl3
Trichlormethan
chloroforme
[H]C(Cl)(Cl)Cl
1,1,1-trichloromethane
chloroformium pro narcosi</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">chloroform</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_35381 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_35381">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_16646"/>
<obo:IAO_0000115 xml:lang="en">Parent monosaccharides are polyhydroxy aldehydes H[CH(OH)]nC(=O)H or polyhydroxy ketones H-[CHOH]n-C(=O)[CHOH]m-H with three or more carbon atoms. The generic term 'monosaccharide' (as opposed to oligosaccharide or polysaccharide) denotes a single unit, without glycosidic connection to other such units. It includes aldoses, dialdoses, aldoketoses, ketoses and diketoses, as well as deoxy sugars, provided that the parent compound has a (potential) carbonyl group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Monosaccharid
Monosacharid
monosaccharides
monosacarido
monosacaridos
Monosaccharide
monosaccharide</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">monosaccharide</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_3567 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_3567">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#QuaternaryAmmoniumSaltReagent"/>
<obo:IAO_0000112 xml:lang="en">cetyltrimethylammonium bromide acts a cationic detergent and is suitable for use in DNA precipitation. CTAB has been used for the isolation of plant high molecular weight DNA as well as plant DNA for use in PCR analysis. It has been utilized to precipitate nucleic acids.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">The organic bromide salt that is the bromide salt of cetyltrimethylammonium; one of the components of the topical antiseptic cetrimide.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Cetyltrimethylammonium bromide
N,N,N-trimethylhexadecan-1-aminium bromide
N-Hexadecyl-N,N,N-trimethylammonium bromide
Cetyl trimethyl ammonium bromide
Bromuro de cetrimonio
n-Hexadecyltrimethylammonium bromide
N,N,N-Trimethylcetylammonium bromide
Cetrimide
Trimethylhexadecylammonium bromide
N-Hexadecyltrimethylammonium bromide
Bromat
Cee dee
Hexadecyl-trimethyl-ammonium bromide
N-Cetyltrimethylammonium bromide
Cetrimonium bromide
Ctmab
hexadecyltrimethylammonium bromide
N,N,N-Trimethyl-1-hexadecanaminium bromide
C19H42BrN
CTAB
Palmityltrimethyl ammonium bromide
C19H42N.Br
Cetrimonii bromidum
Bromure de cetrimonium
cetrimide</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">cetyltrimethylammonium bromide</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_35681 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_35681">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_30879"/>
<obo:IAO_0000115 xml:lang="en">A secondary alcohol is a compound in which a hydroxy group, -OH, is attached to a saturated carbon atom which has two other carbon atoms attached to it.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">secondary alcohols
CH2OR2
a secondary alcohol</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">secondary alcohol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_36078 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_36078">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SteroidReagent"/>
<obo:IAO_0000118 xml:lang="en">cholanoids
bile acids and derivatives</obo:IAO_0000118>
<rdfs:label xml:lang="en">cholanoid</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_36080 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_36080">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33695"/>
<obo:IAO_0000115 xml:lang="en">A biological macromolecule minimally consisting of one polypeptide chain synthesized at the ribosome.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">ChEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">protein</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_36093 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_36093">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ChlorideSaltReagent"/>
<obo:IAO_0000118 xml:lang="en">inorganic chloride salts
inorganic chlorides
inorganic chloride salt</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">inorganic chloride</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_36278 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_36278">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SteroidAcidReagent"/>
<obo:IAO_0000118 xml:lang="en">cholan-24-oic acids</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">cholanic acids</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_38700 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_38700">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SodiumSaltReagent"/>
<obo:IAO_0000118 xml:lang="en">organic sodium salts</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">organic sodium salt</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_41218 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_41218">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_15734"/>
<obo:IAO_0000112 xml:lang="en">2-Mercaptoethanol is used for reducing some proteins. 2-Mercaptoethanol is used in some RNA isolation procedures to eliminate ribonuclease released during cell lysis.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Mercaptoethanol is an alkanethiol that has formula C2H6OS.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Mercaptoethanol
2-sulfanylethanol
Thioglycol
beta-mercaptoethanol
BETA-MERCAPTOETHANOL
2-Mercaptoethanol
2-mercaptoethanol
2-mercapto-ethanol
2-Mercapto-ethanol
2-Mercapto-Ethanol
OCCS
β-mercaptoethanol
BME
2BME
2-ME
β-met
b-mercaptoethanol
b-Mercaptoethanol</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">mercaptoethanol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_4705 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_4705">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_16991"/>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">double-stranded DNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_47704 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_47704">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000118 xml:lang="en">ammonium salts
Ammoniumsalze
Ammoniumsalz</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">ammonium salt</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_48607 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_48607">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_36093"/>
<obo:IAO_0000115 xml:lang="en">A metal chloride salt with a Li(+) counterion.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">LiCl
[Li+].[Cl-]
ClLi
lithii chloridum
cloruro de litio
Lithiumchlorid
chlorure de lithium</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">lithium chloride</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_50699 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_50699">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_16646"/>
<obo:IAO_0000115 xml:lang="en">A compound in which monosaccharide units are joined by glycosidic linkages. The term is commonly used to refer to a defined structure as opposed to a polymer of unspecified length or a homologous mixture. When the linkages are of other types the compounds are regarded as oligosaccharide analogues.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">oligosacarido
an oligosaccharide
O-glycosylglycosides
O-glycosylglycoside
Oligosaccharide
oligosaccharides
oligosacaridos</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">oligosaccharide</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_53422 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_53422">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PolyEthyleneGlycolReagent"/>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A polymer composed of PEG-ylated sorbitan [2-(1,2-dihydroxyethyl)tetrahydrofuran-3,4-diol] normally containing a total of 20 oxyethylene groups, with one or more of the terminal hydroxy groups esterified with a fatty acyl group. They are used as emulsifiers and dispersing agents in some pharmaceuticals products and as defoamers and emulsifiers in some foods.</obo:IAO_0000115>
<obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">polysorbates</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">polysorbate</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_53423 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_53423">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_53422"/>
<obo:IAO_0000112 xml:lang="en">Tween 40 acts as a non-ionic detergent used for cell lysis, nuclei isolation and cell fractionation.</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A polymer composed of PEG-ylated sorbitan, where the total number of poly(ethylene glycol) units is 20 (w + x + y + z = 20) and a single terminal is capped by a palmitoyl group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">polisorbato
Tween 40
C62H122O26
Polysorbate 40
Polyoxyethylene (20) sorbitan monopalmitate
polysorbate
polysorbatum</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">polysorbate 40</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_53424 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_53424">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_53422"/>
<obo:IAO_0000112 xml:lang="en">TWEEN 20 acts as a non-ionic detergent. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A polymer composed of PEG-ylated sorbitan, where the total number of poly(ethylene glycol) units is 20 (w + x + y + z = 20) and a single terminal is capped by a dodecanoyl group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">polysorbate
Polysorbate 20
polysorbatum
Polysorbate
polisorbato
C58H114O26
Tween 20
Polyoxyethylene sorbitan monolaurate
Polyoxyethylene (20) sorbitan monolaurate</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:comment xml:lang="en">example of usage source: http://www.sigmaaldrich.com/catalog/product/sial/p1379?lang=en&region=CO&gclid=CjwKEAjw-vewBRDH1-b52Lig1hkSJACTPfVFKmIFgDeASxRzyjDXAt8-HmEYpgjOYCfQWDgPWdodbhoCRV3w_wcB</rdfs:comment>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">polysorbate 20</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_53425 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_53425">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_53422"/>
<obo:IAO_0000112 xml:lang="en">Polyethylene glycol sorbitan monostearate, or TWEEN 60, has been used in a study to assess encapsulation of functional lipophilic components in surfactant-based colloidal delivery systems. It has also been used in a study to investigate doxorubicin loaded magneto-niosomes for targeted drug delivery.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A polymer composed of PEG-ylated sorbitan, where the total number of poly(ethylene glycol) units is 20 (w + x + y + z = 20) and a single terminal is capped by a stearate group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Tween 60
polyoxyethylene (20) sorbitan monostearate
Polyoxyethylene sorbitan monostearate
Polysorbate 60
Polysorbate
PEG-60 Sorbitan stearate
C64H124O26
polysorbate
polisorbato
polysorbatum</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:comment xml:lang="en">example of usage source: http://www.sigmaaldrich.com/catalog/product/sigma/p1629?lang=en&region=CO</rdfs:comment>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">polysorbate 60</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_53426 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_53426">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_53422"/>
<obo:IAO_0000112 xml:lang="en">Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A polymer composed of PEG-ylated sorbitan, where the total number of poly(ethylene glycol) units is 20 (w + x + y + z = 20) and a single terminal is capped by an oleoyl group.</obo:IAO_0000115>
<obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Polysorbate 80
Tween 80
polysorbate
Polyoxyethylene (20) sorbitan monooleate
polisorbato
Glycol (polysorbate 80)
polysorbatum
Polyethylene oxide sorbitan mono-oleate
Polyoxyethylene sorbitan oleate
C64H124O26
Polysorbate 80
Polyoxyethylene sorbitan monooleate</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">polysorbate 80</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_53514 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_53514">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#CopolymerMacromoleculeReagent"/>
<obo:IAO_0000112 xml:lang="en">Pluronic acts as a non-ionic detergent, protects cells from hydrodynamic damage.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A triblock copolymer composed of a central hydrophobic chain of poly(propylene oxide) flanked by two hydrophilic chains of poly(ethylene oxide).</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">poloxamers
poloxamer
pluronics
PEO-block-PPO-block-PEO
PEO-b-PPO-b-PEO</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">pluronic</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_53515 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_53515">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_53514"/>
<obo:IAO_0000115 xml:lang="en">A triblock copolymer composed of a central hydrophobic chain of poly(propylene oxide) (70 units) flanked by two hydrophilic chains of poly(ethylene oxide) (20 units each). A polymer with the formula HO[CH2CH2O]2O[CH2CH(CH3)O]70[CH2CH2O]2OH.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Polyoxypropylene-polyoxyethylene block copolymer
Poloxamer
Block polyethylene-polypropylene glycol
Block polyoxyethylene-polyoxypropylene
Pluronic P123
Propylene oxide ethylene oxide block polymer
Ethylene glycol-propylene glycol block copolymer</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">pluronic P-123</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_53774 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_53774">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PolyEthyleneGlycolReagent"/>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A polymer consisting of nonylbenzene with a poly(ethylene glycol) moiety attached at position 4.</obo:IAO_0000115>
<obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">alpha-(4-nonylphenyl)-omega-hydroxypoly(oxyethylene)</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">tergitol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_62946 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_62946">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_24840"/>
<obo:IAO_0000115 xml:lang="en">An inorganic sulfate salt obtained by reaction of sulfuric acid with two equivalents of ammonia. A high-melting (decomposes above 280degreeC) white solid which is very soluble in water (70.6 g/100 g water at 0degreeC; 103.8 g/100 g water at 100degreeC), it is widely used as a fertilizer for alkaline soils.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">ammonium sulphate
sulfuric acid, diammonium salt
ammonium sulfate (2:1)
[NH4+].[NH4+].[O-]S([O-])(=O)=O
sulfuric acid ammonium salt (1:2)
mascagnite
(NH4)2SO4
H8N2O4S
diammonium sulfate</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">ammonium sulfate</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_62947 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_62947">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_47704"/>
<obo:IAO_0000118 xml:lang="en">[NH4+].CC([O-])=O
E264
acetic acid, ammonium salt
INS No. 264
C2H7NO2
CH3CO2NH4
ammonium ethanoate
azanium acetate
NH4OAc
CH3COONH4
AcONH4
E-264
E 264</obo:IAO_0000118>
<rdfs:label xml:lang="en">ammonium acetate</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_63016 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_63016">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#AromaticEtherReagent"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Tergitol type NP-40 acts as a non-ionic detergent used for solubilizing proteins while maintaining native subunit structure, enzymatic activity, or other structural functions.</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Poly(ether) macoromolecule comprising a branched nonyl group bonded to one of the ring positions in 2-[phenoxypoly(ethoxy)]ethanol. A commericially available detergent, it is used to help crystallise proteins and extract the cytoplasmic contents of cellular culture.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">C9H13O2
Polyethylene glycol mono(branched p-nonylphenyl) ether
branched alpha-(4-nonylphenyl)-omega-hydroxypoly(oxy-1,2-ethanediyl)
Tergitol-type NP-40
Nonylphenol Ethoxylate
NP40
Tergitol NP40</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">nonyl phenoxypolyethoxylethanol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_64708 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_64708">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">An organic molecular entity containing a single carbon atom (C1).</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">one-carbon compounds</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">one-carbon compound</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_67097 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_67097">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#DisaccharideDerivativeReagent"/>
<obo:IAO_0000112 xml:lang="en">decyl beta-D-maltopyranoside acts as a non-ionic detergent used for solubilizing proteins while maintaining native subunit structure, enzymatic activity, or other structural functions.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A glycoside resulting from attachment of a decyl group to the reducing-end anomeric centre of a beta-maltose molecule.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Decyl β-D-maltopyranoside
Decyl-β-D-maltoside</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">decyl beta-D-maltopyranoside</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_67208 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_67208">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">A ribonucleic acid with two complementary strands, similar to the DNA found in all cells.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">double stranded ribonucleic acid;
dsRNA</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">double-stranded RNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_74035 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_74035">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">A class of small RNA molecules which are comprised of approximately 150 nucleotides and which are found within the nucleus of eukaryotic cells.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">snRNAs;
U-RNA;
small nuclear ribonucleic acids;
U-RNAs;
small nuclear RNAs;
snRNA;
small nuclear ribonucleic acid</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">small nuclear RNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_78708 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_78708">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#AromaticEtherReagent"/>
<obo:IAO_0000112 xml:lang="en">Nonidet P-40 acts as a non-ionic detergent used for solubilizing proteins while maintaining native subunit structure, enzymatic activity, or other structural functions.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A poly(ether) macromolecule that is polyethylene glycol in which one of the terminal hydroxy groups has been converted to the corresponding p-octylphenyl ether. A nonionic, non-denaturing detergent, it is used for solubilising membrane proteins during isolation of membrane-protein complexes.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">(C2H4O)nC14H22O
Nonidet P40
polyethylene glycol mono(4-octylphenyl) ether
octylphenolpoly(ethyleneglycolether)
polyethylene glycol mono(octylphenyl) ether
polyethylene glycol mono(p-octylphenyl) ether
Nonylphenyl-polyethylene glycol</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Nonidet P-40</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_86026 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_86026">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">A threonylcarbamoyladenosine that is incorporated into a tRNA molecule.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en"> tRNA threonylcarbamoyladenosine</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_8984 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_8984">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#AlkylSulfateReagent"/>
<obo:IAO_0000112 xml:lang="en">Sodium dodecyl sulfate acts as a anionic detergent that often completely disrupt cell membranes and fully denature proteins.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">An alkyl sulfate that has formula C12H25NaO4S.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sodium dodecylsulfate
C12H25O4S.Na
C12H25NaO4S
SDS
sodium lauryl sulphate
sodium dodecyl sulphate
SLS
Sodium lauryl sulfate</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">sodium dodecyl sulfate</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_9160 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_9160">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_16991"/>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">single-stranded DNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_9177 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_9177">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#BileAcidSaltReagent"/>
<obo:IAO_0000112 xml:lang="en">Sodium deoxycholate acts as a anionic detergent; useful for extraction of membrane receptors and other plasma membrane proteins and for nuclei isolation.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A bile acid salt that has formula C24H39NaO4.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sodium 7-deoxycholate
C24H39NaO4
desoxycholate sodium
sodium desoxycholate
deoxycholic acid sodium salt
deoxycholate sodium
Sodium deoxycholate
C24H39O4.Na
sodium deoxycholate
sodium 3alpha,12alpha-dihydroxy-5beta-cholan-24-oate</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:comment xml:lang="en">example of usage source: http://www.sigmaaldrich.com/catalog/product/sial/d6750?lang=en&region=CO</rdfs:comment>
<rdfs:label xml:lang="en">sodium deoxycholate</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_9750 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CHEBI_9750">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PolyEthyleneGlycolReagent"/>
<obo:IAO_0000112 xml:lang="en">Triton X-100 acts as a nonionic surfactant used for solubilizing proteins while maintaining native subunit structure, enzymatic activity, or other structural functions.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Triton X-100 is a A poly ethylene glycol terminated with a 4-(2,4,4-trimethylpentan-3-yl) phenyl group at one end.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Poly(oxyethylene)-p-tert-octylphenyl ether
Polyethylene glycol mono(p-(1,1,3,3-tetramethylbutyl)phenyl) ether
octoxinolum
Peg (p-(1,1,3,3-tetramethylbutyl)phenyl) ether
Peg 4-isooctylphenyl ether
Polyoxyethylene mono(octylphenyl) ether alpha-(4-(1,1,3,3-Tetramethylbutyl)phenyl)-omega-hydroxypoly(oxy-1,2-ethanediyl)
(C2H4O)nC14H22O
4-tert-Octylphenyl peg ether
Polyethylene glycol mono(p-tert-octylphenyl) ether
Peg 4-tert-octylphenyl ether
Polyoxyethylene 4-(1,1,3,3-tetramethylbutyl)phenyl ether
4-(1,1,3,3-Tetramethylbutyl)phenyl hydroxypoly(oxyethylene)
octoxinol
Octoxynol 9
Triton X - 100
triton X-100
triton X - 100
triton x-100
triton x - 100
Triton-X 100
triton-X 100</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">Triton X-100</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000020 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000020">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000128"/>
<obo:IAO_0000115 xml:lang="en">a immortal cell line that is dervied from human tissue.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">immortal human cell line</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal human cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000076 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000076">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000128"/>
<obo:IAO_0000115 xml:lang="en">a immortal contractile cell line cell that is derived from muscle cell</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal muscle cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000081 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000081">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000156"/>
<obo:IAO_0000115 xml:lang="en">a immortal electrically responsive cell line cell that is derived from neuron.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal neuron cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000084 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000084">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000081"/>
<obo:IAO_0000115 xml:lang="en">a immortal neuron cell line cell that is derived from peripheral neuron</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal peripheral neuron cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000128 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000128">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000548"/>
<obo:IAO_0000118 xml:lang="en">immortal animal cell line</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal animal cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000129 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000129">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000128"/>
<obo:IAO_0000115 xml:lang="en">a immortal animal cell line that is derived from epithelial cell</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal epithelial cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000156 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000156">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000128"/>
<obo:IAO_0000115 xml:lang="en">a immortal animal cell line that is derived from neural cell</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">immortal neural cell line</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal neural cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000168 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000168">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000636"/>
<obo:IAO_0000115 xml:lang="en">a immortal epithelial cell line cell that is derived from HeLa cell</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">HeLa cell derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000228 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000228">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000128"/>
<obo:IAO_0000115 xml:lang="en">An immortal cell line cell that derives from prostate gland.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal prostate gland-derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000235 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000235">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000128"/>
<obo:IAO_0000115 xml:lang="en">An immortal cell line cell that derives from liver.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal liver-derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000236 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000236">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000128"/>
<obo:IAO_0000115 xml:lang="en">An immortal cell line cell that derives from colon.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal colon-derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000277 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000277">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000128"/>
<obo:IAO_0000115 xml:lang="en">An immortal cell line cell that derives from neural nucleus.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal neural nucleus-derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000283 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000283">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000129"/>
<obo:IAO_0000115 xml:lang="en">An immortal epithelial cell line cell that derives from uterine cervix.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal uterine cervix-derived epithelial cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000513 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000513">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000236"/>
<obo:IAO_0000115 xml:lang="en">An immortal colon-derived cell line cell that derives from human.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal human colon-derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000523 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000523">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000084"/>
<obo:IAO_0000115 xml:lang="en">An immortal peripheral neuron cell line cell that derives from human.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal human peripheral neuron cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000565 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000565">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000235"/>
<obo:IAO_0000115 xml:lang="en">An immortal liver-derived cell line cell that derives from human.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal human liver-derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000623 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000623">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000277"/>
<obo:IAO_0000115 xml:lang="en">An immortal neural nucleus-derived cell line cell that derives from rat.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal rat neural nucleus-derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000636 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000636">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000283"/>
<obo:IAO_0000115 xml:lang="en">An immortal uterine cervix-derived epithelial cell line cell that derives from human.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal human uterine cervix-derived epithelial cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0000748 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000748">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000228"/>
<obo:IAO_0000115 xml:lang="en">An immortal prostate gland-derived cell line cell that derives from human.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">immortal human prostate gland-derived cell line cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0001591 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0001591">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000020"/>
<obo:IAO_0000118 xml:lang="en">A431</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">A431 cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0002140 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0002140">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000623"/>
<obo:IAO_0000118 xml:lang="en">C6</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">disease: glial tumor</rdfs:comment>
<rdfs:label xml:lang="en">C6 cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0003665 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0003665">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000513"/>
<obo:IAO_0000118 xml:lang="en">HCT 116
HCT116
HCT-116</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">HCT 116 cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0003684 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0003684">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000168"/>
<obo:IAO_0000118 xml:lang="en">HeLa</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">HeLa cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0003704 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0003704">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000565"/>
<obo:IAO_0000118 xml:lang="en">Hep G2
Hep-G2
HEPG2
HEP-G2</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">disease: hepatocellular carcinoma</rdfs:comment>
<rdfs:label xml:lang="en">Hep G2 cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0007043 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0007043">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000523"/>
<obo:IAO_0000118 xml:lang="en">JURKAT
jurkat</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">disease: leukemia, T cell</rdfs:comment>
<rdfs:label xml:lang="en">JURKAT
jurkat</rdfs:label>
<rdfs:label xml:lang="en">JURKAT cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0007365 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0007365">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000748"/>
<obo:IAO_0000118 xml:lang="en">LNCaP
LNCAP</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">LNCAP
LNCaP</rdfs:label>
<rdfs:label xml:lang="en">LNCAP cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0050025 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0050025">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000020"/>
<obo:IAO_0000115 xml:lang="en">A immortal human cell line cell that has the characteristics: Lung carcinoma, refer to ATCC CCL185</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">RCB0098
A549</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">RCB0098 cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CLO_0050871 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CLO_0050871">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000076"/>
<obo:IAO_0000115 xml:lang="en">A immortal mouse muscle cell line cell that has the characteristics: Said differentiate to myotubes, but need confirmation.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">C2C12
RCB0987
c2C12</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell line ontology (CLO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">RCB0987 cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CL_0000000 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CL_0000000">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0000671"/>
<obo:IAO_0000115 xml:lang="en">A material entity of anatomical origin (part of or deriving from an organism) that has as its parts a maximally connected cell compartment surrounded by a plasma membrane.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell type ontology (CL)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">The definition of cell is intended to represent all cells, and thus a cell is defined as a material entity and not an anatomical structure, which implies that it is part of an organism (or the entirety of one).</rdfs:comment>
<rdfs:label xml:lang="en">cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CL_0000057 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CL_0000057">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000548"/>
<obo:IAO_0000115 xml:lang="en">A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell type ontology (CL)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.</rdfs:comment>
<rdfs:label xml:lang="en">fibroblast</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CL_0000521 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CL_0000521">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000000"/>
<obo:IAO_0000412 xml:lang="en">cell type ontology (CL)</obo:IAO_0000412>
<rdfs:label xml:lang="en">fungal cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CL_0000530 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CL_0000530">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000540"/>
<obo:IAO_0000412 xml:lang="en">cell type ontology (CL)</obo:IAO_0000412>
<rdfs:label xml:lang="en">primary neuron</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CL_0000540 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CL_0000540">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0002319"/>
<obo:IAO_0000115 xml:lang="en">The basic cellular unit of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the nervous system.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">nerve cell</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">cell type ontology (CL)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">These cells are also reportedly CD4-negative and CD200-positive. They are also capable of producing CD40L and IFN-gamma.</rdfs:comment>
<rdfs:label xml:lang="en">neuron</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CL_0000548 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CL_0000548">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000000"/>
<obo:IAO_0000412 xml:lang="en">cell type ontology (CL)</obo:IAO_0000412>
<rdfs:label xml:lang="en">animal cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CL_0002319 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/CL_0002319">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000548"/>
<obo:IAO_0000115 xml:lang="en">A cell that is part of the nervous system.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">cell type ontology (CL)</obo:IAO_0000412>
<rdfs:label xml:lang="en">neural cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/ERO_0000071 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/ERO_0000071">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<obo:IAO_0000112 xml:lang="en">Microsoft Word is commonly used word processing software.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Software is a general term primarily used for digitally stored data such as computer programs and other kinds of information read and written by computers.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://en.wikipedia.org/wiki/Computer_software</obo:IAO_0000119>
<obo:IAO_0000412 xml:lang="en">ERO</obo:IAO_0000412>
<rdfs:label xml:lang="en">software</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/ERO_0000630 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/ERO_0000630">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000112 xml:lang="en">A primer</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Reagent that is a polymer comprised of nucleotides, each of which consists of three components: a nitrogenous heterocyclic base, which is either a purine or a pyrimidine; a pentose sugar; and a phosphate group.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://en.wikipedia.org/wiki/Nucleic_acid</obo:IAO_0000119>
<obo:IAO_0000412 xml:lang="en">ERO</obo:IAO_0000412>
<rdfs:label xml:lang="en">nucleic acid reagent</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/ERO_0001201 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/ERO_0001201">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/ERO_0000630"/>
<obo:IAO_0000112 xml:lang="en">A primer.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A reagent that is a polymer comprised of DNA nucleotides.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">PERSON: Matthew Brush</obo:IAO_0000119>
<obo:IAO_0000412 xml:lang="en">ERO</obo:IAO_0000412>
<rdfs:label xml:lang="en">DNA reagent</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/ERO_0001203 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/ERO_0001203">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/ERO_0001201"/>
<obo:IAO_0000112 xml:lang="en">A primer.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A DNA reagent consisting of a short, linear DNA polymer, typically with 100 or fewer bases.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">DNA oligo</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">ERO</obo:IAO_0000412>
<rdfs:label xml:lang="en">DNA oligonucleotide</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000028 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/IAO_0000028">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<obo:IAO_0000115 xml:lang="en">An information content entity that is a mark or character used as a conventional representation of another entity.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">IAO</obo:IAO_0000412>
<rdfs:label xml:lang="en">symbol</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000030 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/IAO_0000030">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#GenericallyDependentContinuant"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Examples of information content entites include journal articles, data, graphical layouts, and graphs.</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">an information content entity is an entity that is generically dependent on some artifact and stands in relation of aboutness to some entity</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI_0000142</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">information content entity</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000100 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/IAO_0000100">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000030"/>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Intensity values in a CEL file or from multiple CEL files comprise a data set (as opposed to the CEL files themselves).</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A data item that is an aggregate of other data items of the same type that have something in common. Averages and distributions can be determined for data sets.</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">group:OBI
OBI_0000042</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">data set</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000129 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/IAO_0000129">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000028"/>
<obo:IAO_0000115 xml:lang="en">A version number is an information content entity which is a sequence of characters borne by part of each of a class of manufactured products or its packaging and indicates its order within a set of other products having the same name.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">IAO</obo:IAO_0000412>
<rdfs:label xml:lang="en">version number</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000300 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/IAO_0000300">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000030"/>
<obo:IAO_0000112 xml:lang="en">Words, sentences, paragraphs, and the written (non-figure) parts of publications are all textual entities</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A textual entity is a part of a manifestation (FRBR sense), a generically dependent continuant whose concretizations are patterns of glyphs intended to be interpreted as words, formulas, etc.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">text</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">textual entity</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000310 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/IAO_0000310">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000030"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/IAO_0000314"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000112 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A journal article, patent application, laboratory notebook, or a book</obo:IAO_0000112>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A collection of information content entities intended to be understood together as a whole</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">document</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000314 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/IAO_0000314">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000030"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/IAO_0000310"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000112 xml:lang="en">An abstract, introduction, method or results section.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">an information content entity that is part of a document</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">document part</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000321 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/IAO_0000321">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000314"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000112 xml:lang="en">Lawrence Hunter and Kevin Brettonel Cohen</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">part of a document that enumerates the authors of the document</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">IAO</obo:IAO_0000412>
<rdfs:label xml:lang="en">author list</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/NCBITaxon_10088 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/NCBITaxon_10088">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
<obo:IAO_0000115 xml:lang="en">Mus (commonly mice) is a small mammal belonging to the order of rodents.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">mice
mouse</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">NCBITaxon</obo:IAO_0000412>
<rdfs:label></rdfs:label>
<rdfs:label xml:lang="en">Mus</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/NCBITaxon_10090 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/NCBITaxon_10090">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/NCBITaxon_10088"/>
<obo:IAO_0000118 xml:lang="en">mouse
Mus muscaris
house mouse
mice C57BL/6xCBA/CaJ hybrid</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">NCBITaxon</obo:IAO_0000412>
<rdfs:label xml:lang="en">Mus musculus</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/NCBITaxon_5702 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/NCBITaxon_5702">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/NCBITAXON/39700"/>
<obo:IAO_0000118 xml:lang="en">Trypanosoma (Trypanozoon) brucei brucei
T. b. brucei</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">NCBITaxon</obo:IAO_0000412>
<rdfs:label xml:lang="en">Trypanosoma brucei brucei</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0000136 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0000136">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000112 xml:lang="en">Incubators are used in microbiology for culturing (growing) bacteria and other microorganisms. Incubators in tissue culture rooms are used for culturing stem cells, lymphocytes, skin fibroblasts and other types of cells</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">a device in which environmental conditions (light, photoperiod, temperature, humidity, etc.) can be controlled</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://www.medterms.com/script/main/art.asp?articlekey=18426</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">incubator</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0000671 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0000671">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0000747"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.obolibrary.org/obo/RO_0001000"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SpecimenList"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">a material obtained from an organism in order to be a representative of the whole</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sample
specimen
biospecimen</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">sample from organism</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0000747 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0000747">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000112 xml:lang="en">blood drawn from patient to measure his systemic glucose level. A population of humans with HIV enrolled in a study taken to represent patients with HIV in general.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A material entity that has the material sample role</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sample population</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">material sample</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0000809 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0000809">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProcessedDNA"/>
<obo:IAO_0000112 xml:lang="en">Amplied DNA created by PCR</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">DNA that has been produced in an enzymatic amplification process</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Alan Ruttenberg</obo:IAO_0000119>
<obo:IAO_0000412 xml:lang="en">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">amplified DNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0001051 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0001051">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProcessedDNA"/>
<obo:IAO_0000115 xml:lang="en">The output of an extraction process in which DNA molecules are purified in order to exclude DNA from organellas.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">Group: UPenn group</obo:IAO_0000119>
<obo:IAO_0000412 xml:lang="en">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">DNA extract</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0001087 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0001087">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PhysicalDisruptionEquipmentAndSupplies"/>
<obo:IAO_0000115 xml:lang="en">A device that is used to freeze dry material.</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://en.wikipedia.org/wiki/Freeze_drying</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">lyophilizer</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0001100 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0001100">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0400106"/>
<obo:IAO_0000115 xml:lang="en">A type of centrifuge that is designed for small tubes (0.2 ml to 2.0 ml), has a compact design, and has a small footprint.</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://en.wikipedia.org/wiki/Laboratory_centrifuge</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">microcentrifuge</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0001128 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0001128">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000115 xml:lang="en">A microinjection device that is used to measure very small volumes of liquids.</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://www.answers.com/topic/micropipette</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">micropipette</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0001616 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0001616">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000028"/>
<obo:IAO_0000115 xml:lang="en">A symbol denotes a specimen and used to distinguish one specimen from another in an investigation.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sample identifier</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">specimen identifier</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0100026 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0100026">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SpecimenList"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000112 xml:lang="en">fungus, animal, virus, plant</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A material entity that is an individual living system, such as animal, plant, bacteria or virus, that is capable of replicating or reproducing, growth and maintenance in the right environment. An organism may be unicellular or made up, like humans, of many billions of cells divided into specialized tissues and organs.</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://en.wikipedia.org/wiki/Organism</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">organism</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0400106 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0400106">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000115 xml:lang="en">A device with a rapidly rotating container that applies centrifugal force to its contents</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://en.wikipedia.org/wiki/Centrifuge</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">centrifuge</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0400114 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0400114">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PhysicalDisruptionEquipmentAndSupplies"/>
<obo:IAO_0000112 xml:lang="en">Sonicator 3000</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A device that converts a variable electrical current to mechanical vibration of a metallic probe. The device is used for the lysis of cells, the mixing of compounds or solutions, to framgent molecules of DNA, or to create emulsions.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">sonicator</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0400116 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0400116">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000112 xml:lang="en">Piko(tm) 96-well Thermal Cycler</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">An instrument that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">DNA_amplifier
PCR_machine
Polymerase_Chain_Reaction_ machine
thermocycler</obo:IAO_0000118>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">MO</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">thermal cycler</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0400120 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0400120">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000112 xml:lang="en">VWR Signature Deep-Chamber Heated Water Bath. A water bath is used for temperatures up to 100 degrees C. An oil bath is employed for temperatures over 100 degrees C.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A temperature_control_bath is a device that has the function to regulate the temperature of a material, the function to contain fluid and the function to vary and maintain the temperature of the contained fluid. Heat exchange (energy transfer) between the material and the heating element is facilitated via the contained fluid. A temperature_control_bath is composed of a container, a heating element and/or a cooling element and a means to adjust the needed temperature. In most cases also a timer and a means to stir the fluid is provided as well.</obo:IAO_0000115>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI Instrument branch</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">temperature control bath</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0400155 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0400155">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0400120"/>
<obo:IAO_0000112 xml:lang="en">A water bath was used to allow for cell incubation at 38 degree centigrade for 8 hours.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A water bath is a temperature control bath in which a water acts as contact medium enabling temperature transfer from the heating element or cooling element to the sample. The temperature can be controlled in the 0 to 100 degree centigrade range (under normal pressure).</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">water bath</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0000005 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0000005">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0009002"/>
<obo:IAO_0000115 xml:lang="en">A plant cell that is grown or maintained in vitro.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">cell suspension
cultured cell
plant tissue culture</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">Includes isolated plant cells and small plant cell aggregates that proliferate while suspended in sterile liquid medium or spread on a solid agar medium.</rdfs:comment>
<rdfs:label xml:lang="en">cultured plant cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0000006 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0000006">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0000005"/>
<obo:IAO_0000115 xml:lang="en">A cultured plant cell from which the entire plant cell wall has been removed.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">protoplast</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">The cell wall is generally removed using polysaccharide-degrading enzymes such as cellulase, pectinase and /or xylanase. The cultured plant cell may be in suspension culture or from callus culture on solid media.</rdfs:comment>
<rdfs:label xml:lang="en">plant protoplast</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0000007 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0000007">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0000005"/>
<obo:IAO_0000115 xml:lang="en">A cultured plant cell that was derived from leaf tissue.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">May be derived from cultured plant callus or plant protoplast that was induced from a segment of leaf. In the case of the plant protoplast, after the re-establishment cell walls.</rdfs:comment>
<rdfs:label xml:lang="en">leaf-derived cultured plant cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0000008 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0000008">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0000005"/>
<obo:IAO_0000115 xml:lang="en">A cultured plant cell that was derived from root tissue.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">May be derived from cultured plant callus or plant protoplasts induced from a segment of root. In the case of the plant protoplast, after the re-establishment plant cell walls.</rdfs:comment>
<rdfs:label xml:lang="en">root-derived cultured plant cel</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009002 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0009002">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000000"/>
<obo:IAO_0000115 xml:lang="en">A cell which is a plant structure (PO:0009011).</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">Applies to cells that are living or dead at maturity (e.g., fiber cell or tracheid) and includes any external encapsulating structures (if present) such as the plasma membrane and the plant-type cell wall. Definition of cell GO:0005623: "The basic structural and functional unit of all organisms. Includes the plasma membrane and any external encapsulating structures such as the cell wall and cell envelope". GO:0009505. Definition of plant-type cell wall (GO:0009505): A more or less rigid structure lying outside the cell membrane of a cell and composed of cellulose and pectin and other organic and inorganic substances, synonym; exact: cellulose and pectin-containing cell wall.</rdfs:comment>
<rdfs:label xml:lang="en">plant cell</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009007 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0009007">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C12801"/>
<obo:IAO_0000112 xml:lang="en">roots, leaves, flowers, stems.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A plant structure (PO:0009011) that consists predominantly of similarly specialized plant cells (PO:0009002) of one or more types. </obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">portion of plant tissue
portion of tissue</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">plant tissue</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009010 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0009010">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A multi-tissue plant structure (PO:0025496) that develops from a plant ovule (PO:0020003) and has as parts a plant embryo (PO:0009009) enclosed in a seed coat (PO:0009088).</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">pyrene
diaspore
semilla
seed tissue</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">A seed generally develops from an ovule (PO:0020003) after fertilization, but may develop without fertilization in the case of apogamy (e.g., adventitious embryos or somatic embryos). A seed is a reproductive unit of seed plants (gymnosperms, angiosperms, and fossil pteridosperms).</rdfs:comment>
<rdfs:label xml:lang="en">seed</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009046 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0009046">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A determinate reproductive shoot system that has as part at least one carpel or at least one stamen and does not contain any other determinate shoot system as a part.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">diclinous flower
pistillate flower
staminate flower
basal flower
double flower
female flower
hermaphrodite flower
male flower
perfect flower
imperfect flower
monoclinous flower
flor</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">flower</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009047 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0009047">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A shoot axis (PO:0025029) that is the primary axis of a plant.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">stalk
trunk
cane
caudices
primocane
scape
caudex
bole
primary stem
tallo
tronco
culm;
eje primario
primary axis</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:comment xml:lang="en">Often bears leaves and buds. It is usually above ground, more or less negatively geotropic, and exogenous in origin.</rdfs:comment>
<rdfs:label xml:lang="en">stem</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0025025 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0025025">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A collective plant organ structure (PO:0025007) that produces root apical meristems (PO:0020147) and the plant structures that arise from them.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">root tissue</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">root</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0025034 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0025034">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A phyllome (PO:0006001) that is not associated with a reproductive structure. </obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">hoja
leaf sample
leaves
leaf tissue
leaf material
fresh-leaf tissue
fresh leaf tissue
dried leaf tissue
dried leaf sample
dried leaf
Leaf blade</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">leaf</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0025059 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/PO_0025059">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">Any portion of plant tissue (PO:0009007) other than epidermis (PO:0005679) or vascular tissue (PO:0009015).</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">portion of ground tissue</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<rdfs:label xml:lang="en">ground tissue</rdfs:label>
<rdfs:label xml:lang="en">portion of ground tissue</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/REO_0000045 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/REO_0000045">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_16991"/>
<obo:IAO_0000115 xml:lang="en">a DNA moelcule representing sequence reverse transcribed from a messenger RNA template, in a reaction catalyzed by the enzyme reverse transcriptase and the enzyme DNA polymerase.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">cDNA</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">REO</obo:IAO_0000412>
<rdfs:label xml:lang="en">complementary DNA</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/REO_0000421 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/REO_0000421">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/REO_0000458"/>
<obo:IAO_0000115 xml:lang="en">a nucleoside triphosphate reagent containing three phosphate groups bound to a 2'-deoxyribo- sugar ring</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">dNTP reagent
dNTPs
dNTP mix
Deoxynucleoside triphosphates
deoxynucleoside triphosphates
Deoxynucleotide triphosphates
deoxynucleotide triphosphates
Deoxynucleotide (dNTP) Solution Set
Deoxynucleotide (dNTP) Solution Mix</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">REO</obo:IAO_0000412>
<rdfs:label xml:lang="en">deoxyribonucleoside triphosphate reagent</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/REO_0000458 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/REO_0000458">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">A nucleoside triphosphate reagent is a reagent that is a nucleoside containing three phosphate groups bound to its sugar ring.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">NTP reagent</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">REO</obo:IAO_0000412>
<rdfs:label xml:lang="en">nucleoside triphosphate reagent</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0000948 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/UBERON_0000948">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">A myogenic muscular circulatory organ found in the vertebrate cardiovascular system composed of chambers of cardiac muscle. It is the primary circulatory organ.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">chambered heart
vertebrate heart
Herz
branchial heart
Entire heart</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<rdfs:label xml:lang="en">heart</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0000955 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/UBERON_0000955">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">The brain is the center of the nervous system in all vertebrate, and most invertebrate, animals. Some primitive animals such as jellyfish and starfish have a decentralized nervous system without a brain, while sponges lack any nervous system at all. In vertebrates, the brain is located in the head, protected by the skull and close to the primary sensory apparatus of vision, hearing, balance, taste, and smell[WP].</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<rdfs:label xml:lang="en">brain</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0002048 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/UBERON_0002048">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">Respiration organ that develops as an oupocketing of the esophagus.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">pulmo</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<rdfs:comment xml:lang="en">Snakes and limbless lizards typically possess only the right lung as a major respiratory organ; the left lung is greatly reduced, or even absent. Amphisbaenians, however, have the opposite arrangement, with a major left lung, and a reduced or absent right lung [WP]</rdfs:comment>
<rdfs:label xml:lang="en">lung</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0002106 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/UBERON_0002106">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">the organ that functions to filter blood and to store red corpuscles and platelets</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">lien</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<rdfs:label xml:lang="en">spleen</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0002107 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/UBERON_0002107">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">An exocrine gland which secretes bile and functions in metabolism of protein and carbohydrate and fat, synthesizes substances involved in the clotting of the blood, synthesizes vitamin A, detoxifies poisonous substances, stores glycogen, and breaks down worn-out erythrocytes[GO].</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">jecur
iecur</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<rdfs:label xml:lang="en">liver</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0002113 -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/UBERON_0002113">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">A paired organ of the urinary tract which has the production of urine as its primary function.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">reniculate kidney</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<rdfs:label xml:lang="en">kidney</rdfs:label>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OGI.owl#Author -->
<owl:Class rdf:about="http://purl.obolibrary.org/obo/OGI.owl#Author">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">a human being that bears an author role.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">Ontology for genetic interval</obo:IAO_0000412>
<rdfs:label xml:lang="en">author</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AMVReverseTranscriptase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AMVReverseTranscriptase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ReverseTranscriptase"/>
<obo:IAO_0000115 xml:lang="en">AMV reverse transcriptase is an enzyme from the avian myeloblastosis virus like HIV has two subunits, a 63kDa subunit and a 95kDa subunit.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Avian Myeloblastosis Virus (AMV) Reverse Transcriptase
Avian Myeloblastosis Virus Reverse Transcriptase
Reverse Transcriptase AMV
AMV RT</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://link.springer.com/article/10.1007%2Fs10529-012-0904-9</obo:IAO_0000119>
<rdfs:comment xml:lang="en">AMV reverse transcriptase is an enzyme from the avian myeloblastosis virus like HIV has two subunits, a 63kDa subunit and a 95kDa subunit.
Definition source: http://link.springer.com/article/10.1007%2Fs10529-012-0904-9</rdfs:comment>
<rdfs:label xml:lang="en">AMV reverse transcriptase</rdfs:label>
<rdfs:seeAlso xml:lang="en">Avian Myeloblastosis Virus (AMV) Reverse Transcriptase
Avian Myeloblastosis Virus Reverse Transcriptase
Reverse Transcriptase AMV
AMV RT</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Advantage2PCRBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Advantage2PCRBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PCRBuffer"/>
<obo:IAO_0000115 xml:lang="en">Advantage 2 PCR buffer is a buffer optimized for amplifying genomic DNA, and in particular with products less than 1-2 kb.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Advantage 2 PCR Buffer
10X Advantage 2 SA PCR Buffer
Advantage 2 SA PCR Buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">file:///Users/olgaxgiraldop/Downloads/PT3281-1_PR9Z3414%20(1).pdf</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Advantage 2 PCR buffer is a buffer optimized for amplifying genomic DNA, and in particular with products less than 1-2 kb.
Definition source: file:///Users/olgaxgiraldop/Downloads/PT3281-1_PR9Z3414%20(1).pdf</rdfs:comment>
<rdfs:label xml:lang="en">Advantage 2 PCR buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">Advantage 2 PCR Buffer
10X Advantage 2 SA PCR Buffer
Advantage 2 SA PCR Buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AdvantageOfTheProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AdvantageOfTheProtocol">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PrimerSequence"/>
<obo:IAO_0000115 xml:lang="en">Advantage of the protocol is a subclass of textual entity. It should be made clear in which situations the Protocol has been successfully employed.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://www.nature.com/nprot/info/gta.html</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Advantage of the protocol is a subclass of textual entity. It should be made clear in which situations the Protocol has been successfully employed.
Definition source: http://www.nature.com/nprot/info/gta.html</rdfs:comment>
<rdfs:label xml:lang="en">advantage of the Protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AgaroseGelElectrophoresisProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AgaroseGelElectrophoresisProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ElectrophoresisProtocol"/>
<obo:IAO_0000115 xml:lang="en">Agarose Gel Electrophoresis Protocol is a subclass of laboratory protocol. Agarose Gel Electrophoresis Protocol is a step by step description of a procedure used to separate DNA or RNA molecules by size, achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Agarose Gel Electrophoresis Protocol is a subclass of laboratory protocol. Agarose Gel Electrophoresis Protocol is a step by step description of a procedure used to separate DNA or RNA molecules by size, achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field.</rdfs:comment>
<rdfs:label xml:lang="en">agarose gel electrophoresis protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AlkaliMetalSaltReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AlkaliMetalSaltReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<rdfs:comment xml:lang="en">alkali metal salts usually are very water soluble. Some alkali metal salt are: potasium salt, sodium salt.</rdfs:comment>
<rdfs:label xml:lang="en">alkali metal salt reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AlkanesulfonateReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AlkanesulfonateReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000118 xml:lang="en">an alkanesulfonate
O3RS
alkanesulfonates</obo:IAO_0000118>
<rdfs:label xml:lang="en">alkanesulfonate reagent</rdfs:label>
<rdfs:seeAlso xml:lang="en">an alkanesulfonate
O3RS
alkanesulfonates</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AlkylSulfateReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AlkylSulfateReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000112></obo:IAO_0000112>
<obo:IAO_0000112 xml:lang="en">A sodium dodecyl sulfate.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Alkyl sulfate reagent is a reagent commonly used as an anionic surfactant in liquid soaps, detergents to clean wool, surface cleaners, as active ingredients in laundry detergents, shampoos and conditioners.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">HO4SR
alkyl sulfates</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Organosulfate</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Alkyl sulfate reagent is a reagent commonly used as an anionic surfactant in liquid soaps, detergents to clean wool, surface cleaners, as active ingredients in laundry detergents, shampoos and conditioners.
Definition source: https://en.wikipedia.org/wiki/Organosulfate
Example of usage: A sodium dodecyl sulfate.</rdfs:comment>
<rdfs:label xml:lang="en">alkyl sulfate reagent</rdfs:label>
<rdfs:seeAlso xml:lang="en">HO4SR
alkyl sulfates</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AmpliTaqDNAPolymerase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AmpliTaqDNAPolymerase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#TaqPolymerase"/>
<obo:IAO_0000115 xml:lang="en">AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">https://www.thermofisher.com/order/catalog/product/N8080155</obo:IAO_0000119>
<rdfs:comment xml:lang="en">AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
Definition source: https://www.thermofisher.com/order/catalog/product/N8080155</rdfs:comment>
<rdfs:label xml:lang="en">AmpliTaq DNA Polymerase</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AmpliTaqGold -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AmpliTaqGold">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#TaqPolymerase"/>
<obo:IAO_0000115 xml:lang="en">AmpliTaq Gold is a thermostable DNA polymerase designed to make advanced polymerase Chain Reaction techniques. The key component in AmpliTaq Gold is AmpliTaq® DNA Polymerase (Deoxynucleoside triphosphate: DNA Deoxynucleotidyltransferase, E.C.2.7.7.7).</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">AmpliTaq Gold DNA Polymerase</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://mvz.berkeley.edu/egl/inserts/AmpliTaq%20Gold_041554.pdf</obo:IAO_0000119>
<rdfs:comment xml:lang="en">AmpliTaq Gold is a thermostable DNA polymerase designed to make advanced polymerase Chain Reaction techniques. The key component in AmpliTaq Gold is AmpliTaq® DNA Polymerase (Deoxynucleoside triphosphate: DNA Deoxynucleotidyltransferase, E.C.2.7.7.7).
Definition source: http://mvz.berkeley.edu/egl/inserts/AmpliTaq%20Gold_041554.pdf</rdfs:comment>
<rdfs:label xml:lang="en">AmpliTaq Gold</rdfs:label>
<rdfs:seeAlso xml:lang="en">AmpliTaq Gold DNA Polymerase</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AncientDNA -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AncientDNA">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_16991"/>
<obo:IAO_0000115 xml:lang="en">DNA recovered from ancient biological material (e.g., archaeological and historical skeletal material, mummified tissues, preserved plant remains, ice and permafrost cores).</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en"> NCI Thesaurus</obo:IAO_0000119>
<rdfs:label xml:lang="en">Ancient DNA</rdfs:label>
<rdfs:label xml:lang="en">ancient DNA</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AnimalEuthanizationProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AnimalEuthanizationProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">A animal euthanization protocol is a protocol used in a parasitology experiments and defines instructions on how end the life of animals.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">A animal euthanization protocol is a protocol used in a parasitology experiments and defines instructions on how end the life of animals.</rdfs:comment>
<rdfs:label xml:lang="en">animal euthanization protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ApexTaqDNAPolymerase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ApexTaqDNAPolymerase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#TaqPolymerase"/>
<obo:IAO_0000115 xml:lang="en">Apex Taq DNA Polymerase is a thermostable enzyme. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">APEX Taq polymerase
APEX taq DNA polymerase
Apex taq DNA polymerase</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://corebio.ucsd.edu/4DCGI/Catalog/ProductDetail.html?prod_id=7924</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Apex Taq DNA Polymerase is a thermostable enzyme. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
Definition source: https://corebio.ucsd.edu/4DCGI/Catalog/ProductDetail.html?prod_id=7924</rdfs:comment>
<rdfs:label xml:lang="en">Apex Taq DNA Polymerase</rdfs:label>
<rdfs:seeAlso xml:lang="en">APEX Taq polymerase
APEX taq DNA polymerase
Apex taq DNA polymerase</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ApplicationOfTheProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ApplicationOfTheProtocol">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#CatalogNumber"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#LimitationOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PrimerSequence"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PurposeOfProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<owl:disjointWith rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">The protocol works in northern blot assays using cactus, agave, banana, tomato, Arabidopsis and tobacco. Allwood, J.W., et al., Inter-laboratory reproducibility of fast gas chromatography-electron impact-time of flight mass spectrometry (GC-EI-TOF/MS) based plant metabolomics. Metabolomics, 2009. 5(4): p. 479-496.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Application of the protocol is a subclass of textual entity. Applications of the protocol list the full diversity of the applications of the method and support if is possible to extend the range of applications of the protocol.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Application of the protocol is a subclass of textual entity. Applications of the protocol list the full diversity of the applications of the method and support if is possible to extend the range of applications of the protocol.
Example of usage: The protocol works in northern blot assays using cactus, agave, banana, tomato, Arabidopsis and tobacco. Allwood, J.W., et al., Inter-laboratory reproducibility of fast gas chromatography-electron impact-time of flight mass spectrometry (GC-EI-TOF/MS) based plant metabolomics. Metabolomics, 2009. 5(4): p. 479-496.</rdfs:comment>
<rdfs:label xml:lang="en">application of the protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AromaticEtherReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AromaticEtherReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">Any ether in which the oxygen is attached to at least one aryl substituent.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">aromatic ethers</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Any ether in which the oxygen is attached to at least one aryl substituent.
Definition source: CHEBI</rdfs:comment>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">aromatic ether reagent</rdfs:label>
<rdfs:seeAlso xml:lang="en">aromatic ethers</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AuthorIdentifier -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AuthorIdentifier">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000028"/>
<obo:IAO_0000112 xml:lang="en">orcid ID</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Author identifier is a unique number to all authors as a form of authority control.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Author identifier is a unique number to all authors as a form of authority control.
Example of usage: orcid ID</rdfs:comment>
<rdfs:label xml:lang="en">author identifier</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#AuthorName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#AuthorName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/IAO_0000321"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000112 xml:lang="en">Olga Giraldo</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Author name is a textual entity. Author name is the name of a person or institution. If is applicable, the author name should include the first name and last name.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Author name is a textual entity. Author name is the name of a person or institution. If is applicable, the author name should include the first name and last name.
Example of usage: Olga Giraldo</rdfs:comment>
<rdfs:label xml:lang="en">author name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Bag -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Bag">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000112 xml:lang="en">Laboratory bags: autoclavable biohazard bags, specimen/document zipper bags</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A bag is a supply. A bag is a simple tool in the form of a non-rigid container.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">A bag is a supply. A bag is a simple tool in the form of a non-rigid container.
Example of usage:
Laboratory bags: autoclavable biohazard bags, specimen/document zipper bags</rdfs:comment>
<rdfs:label xml:lang="en">bag</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BeadBeater -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BeadBeater">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">Disruption caused by collision with agitated beads in liquid suspension.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Disruption caused by collision with agitated beads in liquid suspension.</rdfs:comment>
<rdfs:label xml:lang="en">bead beater</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BileAcidReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BileAcidReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_24663"/>
<obo:IAO_0000115 xml:lang="en">Any member of a group of steroid carboxylic acids occuring in bile, where they are present as the sodium salts of their amides with glycine or taurine.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Bile acid
Bile salt
Gallensaeure
bile acids
Gallensaeuren</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Any member of a group of steroid carboxylic acids occuring in bile, where they are present as the sodium salts of their amides with glycine or taurine.</rdfs:comment>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">bile acid reagent</rdfs:label>
<rdfs:seeAlso xml:lang="en">Bile acid
Bile salt
Gallensaeure
bile acids
Gallensaeuren</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BileAcidSaltReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BileAcidSaltReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_36078"/>
<obo:IAO_0000115 xml:lang="en">A salt of a bile acid.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">bile acid salt reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Blender -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Blender">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">blender is an instrument that ensure a homogenized blending of the diluted sample.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">blender is an instrument that ensure a homogenized blending of the diluted sample.</rdfs:comment>
<rdfs:label xml:lang="en">blender</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferAL -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferAL">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000112 xml:lang="en">DNA purification</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Buffer AL is a lysis buffer used during DNA isolation using QIAamp and DNeasy kits.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Buffer AL
AL buffer
AL Buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">file:///Users/olgaxgiraldop/Downloads/HB12500021076579%20PS%20DNY%20Buffer%20AL%200513%20WW.pdf</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Buffer AL is a lysis buffer used during DNA isolation using QIAamp and DNeasy kits.
Definition source:
file:///Users/olgaxgiraldop/Downloads/HB12500021076579%20PS%20DNY%20Buffer%20AL%200513%20WW.pdf
Example of usage:
DNA purification</rdfs:comment>
<rdfs:label xml:lang="en">buffer AL</rdfs:label>
<rdfs:seeAlso xml:lang="en">Buffer AL
AL buffer
AL Buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferAP1 -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferAP1">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000112 xml:lang="en">DNA purification</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Buffer AP1 is a lysis buffer used during isolation of DNA from plant tissue with the DNeasy Plant Mini Kit.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Buffer AP1
AP1 buffer
AP1 Buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://www.calpoly.edu/~bio/ubl/protocols_files/qiaplant.pdf</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Buffer AP1 is a lysis buffer used during isolation of DNA from plant tissue with the DNeasy Plant Mini Kit.
Definition source: http://www.calpoly.edu/~bio/ubl/protocols_files/qiaplant.pdf
Example of usage: DNA purification</rdfs:comment>
<rdfs:label xml:lang="en">buffer AP1</rdfs:label>
<rdfs:seeAlso xml:lang="en">Buffer AP1
AP1 buffer
AP1 Buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferAP2 -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferAP2">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProteinPrecipitationBuffer"/>
<rdfs:label xml:lang="en">buffer AP2</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferATL -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferATL">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000115 xml:lang="en">Buffer ATL is a lysis buffer for use in purification of nucleic acids.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">ATL buffer
ATL Buffer
Buffer ATL</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://www.qiagen.com/es/shop/lab-essentials-and-accessories/buffer-atl/</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Buffer ATL is a lysis buffer for use in purification of nucleic acids.
Definition source:
https://www.qiagen.com/es/shop/lab-essentials-and-accessories/buffer-atl/</rdfs:comment>
<rdfs:label xml:lang="en">buffer ATL</rdfs:label>
<rdfs:seeAlso xml:lang="en">ATL buffer
ATL Buffer
Buffer ATL</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferB1 -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferB1">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000115 xml:lang="en">Buffer B1 (Bacterial Lysis Buffer 1) consists of 50 mM Tris•Cl pH 8.0; 50 mM EDTA pH 8.0; 0.5% Tween 20; 0.5% Triton-X100. Buffer B1 is used in combination with lysozyme or lysostaphin and proteinase K for the efficient lysis of bacteria prior to DNA purification using QIAGEN Genomic-tips.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Bacterial Lysis Buffer 1</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://www.qiagen.com/es/resources/faq?id=569abfa9-c05c-4a20-ae6b-793630da3d55&lang=en</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Buffer B1 (Bacterial Lysis Buffer 1) consists of 50 mM Tris•Cl pH 8.0; 50 mM EDTA pH 8.0; 0.5% Tween 20; 0.5% Triton-X100. Buffer B1 is used in combination with lysozyme or lysostaphin and proteinase K for the efficient lysis of bacteria prior to DNA purification using QIAGEN Genomic-tips.
Definition source:
https://www.qiagen.com/es/resources/faq?id=569abfa9-c05c-4a20-ae6b-793630da3d55&lang=en</rdfs:comment>
<rdfs:label xml:lang="en">buffer B1</rdfs:label>
<rdfs:seeAlso xml:lang="en">Bacterial Lysis Buffer 1</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferB2 -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferB2">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000115 xml:lang="en">Buffer B2 (Bacterial Lysis Buffer 2) consists of 3 M guanidine hydrochloride, 20% Tween 20. Buffer B2 is used in combination with Buffer B1, lysozyme or lysostaphin and Proteinase K for efficient lysis of bacteria prior to DNA purification using QIAGEN Genomic-tips.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Bacterial Lysis Buffer 2</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://www.qiagen.com/es/resources/faq?id=92b08ae8-b395-4961-b71b-b1e784c7c8ea&lang=en</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Buffer B2 (Bacterial Lysis Buffer 2) consists of 3 M guanidine hydrochloride, 20% Tween 20. Buffer B2 is used in combination with Buffer B1, lysozyme or lysostaphin and Proteinase K for efficient lysis of bacteria prior to DNA purification using QIAGEN Genomic-tips.
Definition source:
https://www.qiagen.com/es/resources/faq?id=92b08ae8-b395-4961-b71b-b1e784c7c8ea&lang=en</rdfs:comment>
<rdfs:label xml:lang="en">buffer B2</rdfs:label>
<rdfs:seeAlso xml:lang="en">Bacterial Lysis Buffer 2</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferC1 -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferC1">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000115 xml:lang="en">cell lysis buffer</obo:IAO_0000115>
<rdfs:comment xml:lang="en">cell lysis buffer</rdfs:comment>
<rdfs:label xml:lang="en">buffer C1</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PrimerName"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#SoftwareName"/>
<obo:IAO_0000112 xml:lang="en">1X Queen’s lysis buffer (1L),
AB lysis buffer (500ml).</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Buffer name is a textual entity. Buffer name is the name of the buffer produced by or used in a reaction involving changes in atoms or molecules. If is applicable, the name of the buffer should be including the follow information: buffer concentration, and final volume.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Buffer name is a textual entity. Buffer name is the name of the buffer produced by or used in a reaction involving changes in atoms or molecules. If is applicable, the name of the buffer should be including the follow information: buffer concentration, and final volume.
Example of usage:
1X Queen’s lysis buffer (1L),
AB lysis buffer (500ml).</rdfs:comment>
<rdfs:label xml:lang="en">buffer name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#BufferY1 -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#BufferY1">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000115 xml:lang="en">yeast lysis buffer</obo:IAO_0000115>
<rdfs:comment xml:lang="en">yeast lysis buffer</rdfs:comment>
<rdfs:label xml:lang="en">buffer Y1</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CTABBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CTABBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000112 xml:lang="en">DNA purification</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">CTAB buffer is a buffer used during the extraction/isolation of highly polymerized DNA from plant material. The main chemical compound, CTAB, simultaneously solubilizes the plant cell wall and lipid membranes of internal organelles and denatures proteins (enzymes).</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">CTAB extraction buffer
CTAB DNA extraction buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">PERSON:Olga Giraldo</obo:IAO_0000119>
<rdfs:comment xml:lang="en">CTAB buffer is a buffer used during the extraction/isolation of highly polymerized DNA from plant material. The main chemical compound, CTAB, simultaneously solubilizes the plant cell wall and lipid membranes of internal organelles and denatures proteins (enzymes).
Example of usage: DNA purification</rdfs:comment>
<rdfs:label xml:lang="en">CTAB buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">CTAB extraction buffer
CTAB DNA extraction buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CatalogNumber -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CatalogNumber">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000028"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#LimitationOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PrimerSequence"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PurposeOfProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<owl:disjointWith rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">5000-0012</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Catalog number is a subclass of textual entity. Catalog number is a numeral or string of numerals that is used for identification.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Catalog number is a subclass of textual entity. Catalog number is a numeral or string of numerals that is used for identification.
Example of usage: 5000-0012</rdfs:comment>
<rdfs:label xml:lang="en">catalog number</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CellDisruption -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CellDisruption">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolInstruction"/>
<obo:IAO_0000115 xml:lang="en">Breaking the cells open, is the first basic step in a DNA extraction. This is commonly achieved by chemical and physical methods-blending, grinding or sonicating the sample.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Cell lysis</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Breaking the cells open, is the first basic step in a DNA extraction. This is commonly achieved by chemical and physical methods-blending, grinding or sonicating the sample.</rdfs:comment>
<rdfs:label xml:lang="en">cell disruption</rdfs:label>
<rdfs:seeAlso xml:lang="en">Cell lysis</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CentrifugeTube -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CentrifugeTube">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000112 xml:lang="en">Weigh 300-400 mg of ground, lyophilized tissue, into a 15 ml polypropylene centrifuge tube.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Centrifuge tube is a supply. centrifuge tubes are tapered tubes of various sizes made of glass or plastic. They may vary in capacity from tens of millilitres.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">centrifuge tips</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://en.wikipedia.org/wiki/Laboratory_centrifuge#Centrifuge_tubes</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Centrifuge tube is a supply. centrifuge tubes are tapered tubes of various sizes made of glass or plastic. They may vary in capacity from tens of millilitres.
Definition source:
http://en.wikipedia.org/wiki/Laboratory_centrifuge#Centrifuge_tubes
Example of usage:
Weigh 300-400 mg of ground, lyophilized tissue, into a 15 ml polypropylene centrifuge tube</rdfs:comment>
<rdfs:label xml:lang="en">centrifuge tube</rdfs:label>
<rdfs:seeAlso xml:lang="en">centrifuge tips</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ChlorideSaltReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ChlorideSaltReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000118 xml:lang="en">chloride salts
chlorides</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Chloride salts such as sodium chloride are often very soluble in water. It is an essential electrolyte located in all body fluids responsible for maintaining acid/base balance, transmitting nerve impulses and regulating fluid in and out of cells.</rdfs:comment>
<rdfs:label xml:lang="en">chloride salt reagent</rdfs:label>
<rdfs:seeAlso xml:lang="en">chloride salts
chlorides</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ChloroformIsoamylAlcoholMixture -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ChloroformIsoamylAlcoholMixture">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830"/>
<obo:IAO_0000115 xml:lang="en">mixture suitable for nucleic acid purification in plants.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">mixture suitable for nucleic acid purification in plants.</rdfs:comment>
<rdfs:label xml:lang="en">Chloroform:Isoamyl alcohol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CoffeMill -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CoffeMill">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">Coffee mill is an instrument used (during the first stage of nucleic acids purification), to crush leaf tissue to make leaf powder.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">coffee grinder</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Coffee mill is an instrument used (during the first stage of nucleic acids purification), to crush leaf tissue to make leaf powder.</rdfs:comment>
<rdfs:label xml:lang="en">coffee mill</rdfs:label>
<rdfs:seeAlso xml:lang="en">coffee grinder</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CopolymerMacromoleculeReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CopolymerMacromoleculeReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">A macromolecule derived from two or more species of monomer.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">copolymers
co-polymers
copolymer
co-polymer
heteropolymers
heteropolymer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">copolymer macromolecule reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CriticalStep -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CriticalStep">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">Remove the combs and flush out the wells using a syringe. This is a CRITICAL STEP, especially for polymorphic bands that are close to each other. Otherwise, unpolymerized acrylamide solution will be polymerized at the bottom of the wells and will affect the migration of the fragments. (Laboratory Protocols CIMMYT, 2005)
CRITICAL STEP: The supernatant in each step should be carefully transferred without disturbing the other layers. (Sudha, 2012)</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A critical step is a message given to a user to perform a protocol instruction in a very precise manner e.g., where the time and temperature of a step is crucial or the use of RNase free solutions is required.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">A critical step is a message given to a user to perform a protocol instruction in a very precise manner e.g., where the time and temperature of a step is crucial or the use of RNase free solutions is required.
Example of usage: Remove the combs and flush out the wells using a syringe. This is a CRITICAL STEP, especially for polymorphic bands that are close to each other. Otherwise, unpolymerized acrylamide solution will be polymerized at the bottom of the wells and will affect the migration of the fragments. (Laboratory Protocols CIMMYT, 2005)
CRITICAL STEP: The supernatant in each step should be carefully transferred without disturbing the other layers. (Sudha, 2012)</rdfs:comment>
<rdfs:label xml:lang="en">critical step</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CulturedAnimalCell -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CulturedAnimalCell">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000548"/>
<obo:IAO_0000115 xml:lang="en">An animal cell that is grown or maintained in vitro.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">cell suspension
cultured cell
animal tissue culture</obo:IAO_0000118>
<rdfs:comment xml:lang="en">An animal cell that is grown or maintained in vitro.</rdfs:comment>
<rdfs:label xml:lang="en">cultured animal cell</rdfs:label>
<rdfs:seeAlso xml:lang="en">cell suspension
cultured cell
animal tissue culture</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CulturedMammalianCell -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CulturedMammalianCell">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#CulturedAnimalCell"/>
<obo:IAO_0000118 xml:lang="en">cultured mammalian cells</obo:IAO_0000118>
<rdfs:label xml:lang="en">cultured mammalian cell</rdfs:label>
<rdfs:seeAlso xml:lang="en">cultured mammalian cells</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CytoplasmicExtractionBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#CytoplasmicExtractionBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:allValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SubcellularProteinFractionationKitforTissues"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">The cytoplasmic extraction buffer is part of the Subcellular Protein Fractionation Kit for Tissues. This buffer causes selective membrane permeabilization, releasing soluble cytoplasmic contents.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">CEB
CEB buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://www.thermofisher.com/es/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/subcellular-protein-fractionation.html</obo:IAO_0000119>
<rdfs:comment xml:lang="en">The cytoplasmic extraction buffer is part of the Subcellular Protein Fractionation Kit for Tissues. This buffer causes selective membrane permeabilization, releasing soluble cytoplasmic contents.
Definition source:
https://www.thermofisher.com/es/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/subcellular-protein-fractionation.html</rdfs:comment>
<rdfs:label xml:lang="en">cytoplasmic extraction buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">CEB
CEB buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DNAExtractionProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#DNAExtractionProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#NucleicAcidExtractionProtocol"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#CellDisruption"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#DenaturationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#PrecipitationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#WashingNucleicAcids"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#RNAExtractionProtocol"/>
<obo:IAO_0000115 xml:lang="en">DNA extraction protocol is a subclass of nucleic acid extraction protocol. DNA extraction protocol, is a step by step description of a procedure in which is recovered a fraction of DNA from a material entity.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">DNA isolation protocol</obo:IAO_0000118>
<rdfs:comment xml:lang="en">DNA extraction protocol is a subclass of nucleic acid extraction protocol. DNA extraction protocol, is a step by step description of a procedure in which is recovered a fraction of DNA from a material entity.</rdfs:comment>
<rdfs:label xml:lang="en">DNA extraction protocol</rdfs:label>
<rdfs:seeAlso xml:lang="en">DNA isolation protocol</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DNAPellet -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#DNAPellet">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProcessedDNA"/>
<obo:IAO_0000115 xml:lang="en">A material entity which results from the aggregation of DNA produced by the application of centrifugal force to a liquid containing DNA in suspension.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">Adapted from OBI</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A material entity which results from the aggregation of DNA produced by the application of centrifugal force to a liquid containing DNA in suspension.
Definition source: Adapted from OBI</rdfs:comment>
<rdfs:label xml:lang="en">DNA pellet</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DNASeparatedByMolecularSize -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#DNASeparatedByMolecularSize">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProcessedDNA"/>
<obo:IAO_0000115 xml:lang="en">DNA separated by molecular size is obtained after the aplication of electrical potential to move material through a defined matrix.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">DNA separated by molecular size is obtained after the aplication of electrical potential to move material through a defined matrix.</rdfs:comment>
<rdfs:label xml:lang="en">DNA separated by molecular size</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DenaturationReaction -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#DenaturationReaction">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolInstruction"/>
<obo:IAO_0000115 xml:lang="en">The biological activity of a biopolymer is closely linked to its native structure which is retained only if critical factors such as temperature, pH, solvent and solute are kept within narrow limits. Any alteration of the native structure causing a loss of biological activity is called denaturation.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://www.sigmaaldrich.com/life-science/metabolomics/bioultra-reagents/denaturation.html#sthash.5rKMTmls.dpuf</obo:IAO_0000119>
<rdfs:comment xml:lang="en">The biological activity of a biopolymer is closely linked to its native structure which is retained only if critical factors such as temperature, pH, solvent and solute are kept within narrow limits. Any alteration of the native structure causing a loss of biological activity is called denaturation.
Definition source: http://www.sigmaaldrich.com/life-science/metabolomics/bioultra-reagents/denaturation.html#sthash.5rKMTmls.dpuf</rdfs:comment>
<rdfs:label xml:lang="en">denaturation reaction</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DisaccharideDerivativeReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#DisaccharideDerivativeReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">A carbohydrate derivative that is formally obtained from a disaccharide.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">disaccharide derivatives</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">disaccharide derivative reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DisaccharideReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#DisaccharideReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_50699"/>
<obo:IAO_0000115 xml:lang="en">A compound in which two monosaccharides are joined by a glycosidic bond.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Disaccharide
disaccharides
Disaccharid
disacarido
disacaridos
Disacharid</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">disaccharide reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Dithiothreitol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Dithiothreitol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#GlycolReagent"/>
<obo:IAO_0000112 xml:lang="en">DTT is used as a reducing or "deprotecting" agent for thiolated DNA. DTT is frequently used to reduce the disulfide bonds of proteins. DTT can also be used as an oxidizing agent.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Dithiothreitol (DTT) is the common name for a small-molecule redox reagent also known as Cleland's reagent. DTT's formula is C4H10O2S2. Its name derives from the four-carbon sugar, threose. DTT has an epimeric ('sister') compound, dithioerythritol (DTE).</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">rel-(2R,3R)-1,4-disulfanylbutane-2,3-diol
1,4-Dithiothreitol
1,4-dithiothreitol
Cleland's reagent
rac-Dithiothreitol
threo-1,4-Dimercapto-2,3-butanediol
DTT
(R*,R*)-1,4-dimercapto-2,3-butanediol
Dithiothreitol
Dithiotreitol
C4H10O2S2
DL-threo-1,4-Dimercapto-2,3-butanediol
DTL</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Dithiothreitol</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Dithiothreitol (DTT) is the common name for a small-molecule redox reagent also known as Cleland's reagent. DTT's formula is C4H10O2S2. Its name derives from the four-carbon sugar, threose. DTT has an epimeric ('sister') compound, dithioerythritol (DTE).
Definition source: https://en.wikipedia.org/wiki/Dithiothreitol
Example of usage:
DTT is used as a reducing or "deprotecting" agent for thiolated DNA. DTT is frequently used to reduce the disulfide bonds of proteins. DTT can also be used as an oxidizing agent.</rdfs:comment>
<rdfs:label xml:lang="en">dithiothreitol</rdfs:label>
<rdfs:seeAlso xml:lang="en">rel-(2R,3R)-1,4-disulfanylbutane-2,3-diol
1,4-Dithiothreitol
1,4-dithiothreitol
Cleland's reagent
rac-Dithiothreitol
threo-1,4-Dimercapto-2,3-butanediol
DTT
(R*,R*)-1,4-dimercapto-2,3-butanediol
Dithiothreitol
Dithiotreitol
C4H10O2S2
DL-threo-1,4-Dimercapto-2,3-butanediol
DTL</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DounceHomogenizer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#DounceHomogenizer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Homogenizer"/>
<obo:IAO_0000115 xml:lang="en">Shearing caused by a round glass pestle that is manually driven into a glass tube</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">tissue grinder</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Shearing caused by a round glass pestle that is manually driven into a glass tube</rdfs:comment>
<rdfs:label xml:lang="en">dounce homogenizer</rdfs:label>
<rdfs:seeAlso xml:lang="en">tissue grinder</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Drill -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Drill">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">a tool or machine with a rotating cutting tip or reciprocating hammer or chisel, used for making holes.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">drilling tool,
boring tool,
rotary tool,
auger,
gimlet,
awl,
bradawl,
hydraulic drill</obo:IAO_0000118>
<rdfs:comment xml:lang="en">a tool or machine with a rotating cutting tip or reciprocating hammer or chisel, used for making holes.</rdfs:comment>
<rdfs:label xml:lang="en">drill</rdfs:label>
<rdfs:seeAlso xml:lang="en">drilling tool,
boring tool,
rotary tool,
auger,
gimlet,
awl,
bradawl,
hydraulic drill</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ElectrophoresisProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ElectrophoresisProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">Electrophoresis protocol is a subclass of laboratory protocol. Electrophoresis protocol is a step by step description of an electrophoresis procedure in which are recovered fragments of biomolecules separated by charge.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Electrophoresis protocol is a subclass of laboratory protocol. Electrophoresis protocol is a step by step description of an electrophoresis procedure in which are recovered fragments of biomolecules separated by charge.</rdfs:comment>
<rdfs:label xml:lang="en">electrophoresis protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#EndopeptidaseK -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#EndopeptidaseK">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#EnzymeReagent"/>
<obo:IAO_0000112 xml:lang="en">Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">An enzyme that catalyzes the hydrolysis of keratin, and of other proteins with subtilisin-like specificity. It hydrolyses peptide amides. Endopeptidase K is from the mold Tritirachium album Limber.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Proteinase K
Endopeptidase K
Protease K
Tritirachium Alkaline Proteinase
Tritirachium album serine proteinase
Tritirachium album proteinase K</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">National Drug File-Reference Terminology</obo:IAO_0000119>
<rdfs:comment xml:lang="en">An enzyme that catalyzes the hydrolysis of keratin, and of other proteins with subtilisin-like specificity. It hydrolyses peptide amides. Endopeptidase K is from the mold Tritirachium album Limber.
Definition source: National Drug File-Reference Terminology
Example of usage:
Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid.</rdfs:comment>
<rdfs:label xml:lang="en">endopeptidase K</rdfs:label>
<rdfs:seeAlso xml:lang="en">Proteinase K
Endopeptidase K
Protease K
Tritirachium Alkaline Proteinase
Tritirachium album serine proteinase
Tritirachium album proteinase K</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#EnzimaticAmplificationProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#EnzimaticAmplificationProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">Enzimatic amplification protocol is a subclass of laboratory protocol. Enzimatic amplification protocol, is a step by step description of a procedure about the use of enzymes to increase the number of molecules from a material entity.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Enzimatic amplification protocol is a subclass of laboratory protocol. Enzimatic amplification protocol, is a step by step description of a procedure about the use of enzymes to increase the number of molecules from a material entity.</rdfs:comment>
<rdfs:label xml:lang="en">enzimatic amplification protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#EnzymeReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#EnzymeReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">Enzymes are biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Biocatalysts
Enzymes</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">MESH</obo:IAO_0000119>
<rdfs:label xml:lang="en">enzyme reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#EquipmentAndSuppliesList -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#EquipmentAndSuppliesList">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000100"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ReagentList"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#SoftwareList"/>
<obo:IAO_0000115 xml:lang="en">Equipment and supplies list is a data set of the names or identifiers of the equipments and supplies that are part of materials section of a protocol.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Equipment and supplies list is a data set of the names or identifiers of the equipments and supplies that are part of materials section of a protocol.</rdfs:comment>
<rdfs:label xml:lang="en">equipment and supplies list</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentAndSuppliesList"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PrimerName"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ReagentName"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#SoftwareName"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#SolutionName"/>
<obo:IAO_0000112 xml:lang="en">Nalgene Cryogenic Tube, sterile, 1.2 ml.
S1000 Thermal Cycler with 96-Deep Well Reaction Module.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Equipment or supply name is a textual entity. Equipment or supply name is the name of equipment or supply.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Equipment or supply name is a textual entity. Equipment or supply name is the name of equipment or supply.
Example of usage:
Nalgene Cryogenic Tube, sterile, 1.2 ml.
S1000 Thermal Cycler with 96-Deep Well Reaction Module.</rdfs:comment>
<rdfs:label xml:lang="en">equipment or supplies name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#EthanolSolution -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#EthanolSolution">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830"/>
<obo:IAO_0000112 xml:lang="en">70% ethanol</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A homogeneous mixture of ethanol and water.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://cameochemicals.noaa.gov/chemical/19240</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A homogeneous mixture of ethanol and water.
Definition source: http://cameochemicals.noaa.gov/chemical/19240
Example of usage: 70% ethanol</rdfs:comment>
<rdfs:label xml:lang="en">ethanol solution</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ExperimentalProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ExperimentalProtocol">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000310"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAdvantage"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#AdvantageOfTheProtocol"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAlertMessage"/>
<owl:someValuesFrom rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasApplication"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ApplicationOfTheProtocol"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasExperimentalInputs"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentAndSuppliesList"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasExperimentalInputs"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ReagentList"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasExperimentalInputs"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#RecipeList"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasExperimentalInputs"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SoftwareList"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasExperimentalInputs"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SpecimenList"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasIdentifier"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolIdentifier"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolInstruction"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasLimitation"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#LimitationOfTheProtocol"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasOutcome"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#OutcomeOfTheProtocol"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasPurpose"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#PurposeOfProtocol"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasTitle"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#locatedIn"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#RepositoryName"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#publishedIn"/>
<owl:someValuesFrom rdf:resource="http://prismstandard.org/namespaces/basic/2.0/prism:publicationName"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/pav/hasVersion"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/IAO_0000129"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://prismstandard.org/namespaces/basic/2.0/prism:coverDate"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://prismstandard.org/namespaces/basic/2.0/prism:keyword"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/IAO_0000321"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ReferenceList"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">An experimental protocol is a sequence of tasks and operations executed to perform experimental research. The protocols often include equipment, reagents, critical steps, troubleshooting, tips and all the information that facilitates reusability.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">An experimental protocol is a sequence of tasks and operations executed to perform experimental research. The protocols often include equipment, reagents, critical steps, troubleshooting, tips and all the information that facilitates reusability.</rdfs:comment>
<rdfs:label xml:lang="en">experimental protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#FinalConcentration -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#FinalConcentration">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C41185"/>
<obo:IAO_0000115 xml:lang="en">The final measured concentration of a compound in a substance.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Last concentration</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">Adopted from NCI thesaurus</obo:IAO_0000119>
<rdfs:comment xml:lang="en">The final measured concentration of a compound in a substance.
Definition source: Adopted from NCI thesaurus</rdfs:comment>
<rdfs:label xml:lang="en">final concentration</rdfs:label>
<rdfs:seeAlso xml:lang="en">Last concentration</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Flammable -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Flammable">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122"/>
<obo:IAO_0000112 xml:lang="en">Keep away from sources of ignition. Reference: Nature Protocols</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Easily ignited.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Inflammable</obo:IAO_0000118>
<obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://wordnetweb.princeton.edu/perl/webwn?s=flammable</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Easily ignited.
Definition source: http://wordnetweb.princeton.edu/perl/webwn?s=flammable
Example of usage: Keep away from sources of ignition.</rdfs:comment>
<rdfs:label xml:lang="en">flammable</rdfs:label>
<rdfs:seeAlso xml:lang="en">Inflammable</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#FreezerMilling -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#FreezerMilling">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">Freezer milling is a type of cryogenic milling that uses a solenoid to mill samples. The solenoid moves the grinding media back and forth inside the vial, grinding the sample down to analytical fineness.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Cryogenic grinding,
freezer grinding,
cryomilling,
freezer mill</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Freezer milling is a type of cryogenic milling that uses a solenoid to mill samples. The solenoid moves the grinding media back and forth inside the vial, grinding the sample down to analytical fineness.</rdfs:comment>
<rdfs:label xml:lang="en">freezer milling</rdfs:label>
<rdfs:seeAlso xml:lang="en">Cryogenic grinding,
freezer grinding,
cryomilling,
freezer mill</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#FrenchPressureCellPress -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#FrenchPressureCellPress">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">Shearing is caused by high pressure when sample is forced through a small hole.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">french press</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Shearing is caused by high pressure when sample is forced through a small hole.</rdfs:comment>
<rdfs:label xml:lang="en">french pressure cell press</rdfs:label>
<rdfs:seeAlso xml:lang="en">french press</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#GeneAmpPCRBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#GeneAmpPCRBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PCRBuffer"/>
<obo:IAO_0000115 xml:lang="en">GeneAmp PCR Buffer is optimized for use with the enzyme AmpliTaq DNA Polymerase.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">GeneAmp 10X PCR Buffer I
GeneAmp 10X PCR Buffer II
GeneAmp10X PCR Gold Buffer
GeneAmp PCR Buffer I
GeneAmp PCR Buffer II
GeneAmp PCR Gold Buffer</obo:IAO_0000118>
<rdfs:comment xml:lang="en">GeneAmp PCR Buffer is optimized for use with the enzyme AmpliTaq DNA Polymerase.</rdfs:comment>
<rdfs:label xml:lang="en">GeneAmp PCR Buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">GeneAmp 10X PCR Buffer I
GeneAmp 10X PCR Buffer II
GeneAmp10X PCR Gold Buffer
GeneAmp PCR Buffer I
GeneAmp PCR Buffer II
GeneAmp PCR Gold Buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#GeneSpecificPrimer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#GeneSpecificPrimer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">A gene-specific primer is a short strand of nucleic acid that hybridizes only to sequences exactly complementary to the primer sequence.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">gene-specific primers
Gene-Specific Primer
Gene-specific primers
Gene-specific primer
gene specific primers
gene specific primer
Gene specific primers
Gene specific primer
Gene Specific Primer
GSP
Sequence Specific Primers
Sequence-Specific Primers
SSPs</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">PERSON: Olga Giraldo</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A gene-specific primer is a short strand of nucleic acid that hybridizes only to sequences exactly complementary to the primer sequence.</rdfs:comment>
<rdfs:label xml:lang="en">gene-specific primer</rdfs:label>
<rdfs:seeAlso xml:lang="en">gene-specific primers
Gene-Specific Primer
Gene-specific primers
Gene-specific primer
gene specific primers
gene specific primer
Gene specific primers
Gene specific primer
Gene Specific Primer
GSP
Sequence Specific Primers
Sequence-Specific Primers
SSPs</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#GenoGrinder -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#GenoGrinder">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">Geno grinder is a plant and animal tissue homogenizer used for high-throughput applications involving sample preparation for DNA, RNA, and protein extractions, pesticide residue analysis, and more.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Geno/Grinder
GenoGrinder
GenoGrinder machine
genogrinder
genogrinder machine</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Geno grinder is a plant and animal tissue homogenizer used for high-throughput applications involving sample preparation for DNA, RNA, and protein extractions, pesticide residue analysis, and more.</rdfs:comment>
<rdfs:label xml:lang="en">geno grinder</rdfs:label>
<rdfs:seeAlso xml:lang="en">Geno/Grinder
GenoGrinder
GenoGrinder machine
genogrinder
genogrinder machine</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#GlycolReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#GlycolReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">A diol in which the two hydroxy groups are on different carbon atoms, usually but not necessarily adjacent.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Glykol
glykol
glycols</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">glycol reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#GrindingMachine -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#GrindingMachine">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000112 xml:lang="en">Put the number of seed indicated by the kit protocol in the grinding machine and grind the seed to a powder.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Grinding machine is a equipment. Grinding machine is a machine used to grind grains and leaves.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Grinding device</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Grinding machine is a equipment. Grinding machine is a machine used to grind grains and leaves.
Example of usage:
Put the number of seed indicated by the kit protocol in the grinding machine and grind the seed to a powder.</rdfs:comment>
<rdfs:label xml:lang="en">grinding machine</rdfs:label>
<rdfs:seeAlso xml:lang="en">Grinding device</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#GuanidiniumThiocyanatePhenol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#GuanidiniumThiocyanatePhenol">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830"/>
<obo:IAO_0000115 xml:lang="en">guanidinium thiocyanate-phenol is a mixture used in RNA/DNA/protein extraction.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">guanidine thiocyanate-phenol
guanidine thiocyanate-phenol solution
guanidinium thiocyanate-phenol solution
TRIzol
TRI reagent
Trisure
STAT-60</obo:IAO_0000118>
<rdfs:comment xml:lang="en">guanidinium thiocyanate-phenol is a mixture used in RNA/DNA/protein extraction.</rdfs:comment>
<rdfs:label xml:lang="en">guanidinium thiocyanate-phenol</rdfs:label>
<rdfs:seeAlso xml:lang="en">guanidine thiocyanate-phenol
guanidine thiocyanate-phenol solution
guanidinium thiocyanate-phenol solution
TRIzol
TRI reagent
Trisure
STAT-60</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Hint -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Hint">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">HINT: Mix well by vortex before to use.
HINT: ADD NaOH to WATER to avoid splashing.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A hint is a message given to a user of a protocol that will help to perform the experiment.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">A hint is a message given to a user of a protocol that will help to perform the experiment.
Example of usage:
HINT: Mix well by vortex before to use.
HINT: ADD NaOH to WATER to avoid splashing.</rdfs:comment>
<rdfs:label xml:lang="en">hint</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#HomogenizedTissue -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#HomogenizedTissue">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C12801"/>
<obo:IAO_0000115 xml:lang="en">homogenized tissue is a tissue that is brought to a state such that all fractions of the tissue are equal in composition.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">homogenized tissue is a tissue that is brought to a state such that all fractions of the tissue are equal in composition.</rdfs:comment>
<rdfs:label xml:lang="en">homogenized tissue</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Homogenizer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Homogenizer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">A homogenizer is a equipment of laboratory used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others. Many different models have been developed using various physical technologies for disruption.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Homogenizer</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A homogenizer is a equipment of laboratory used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others. Many different models have been developed using various physical technologies for disruption.
Definition source: https://en.wikipedia.org/wiki/Homogenizer</rdfs:comment>
<rdfs:label xml:lang="en">homogenizer</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#HotStartTaqDNAPolymerase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#HotStartTaqDNAPolymerase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#TaqPolymerase"/>
<obo:IAO_0000115 xml:lang="en">Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">HotStarTaq DNA Polymerase
HotStarTaq
Hot Start DNA Polymerase
Hot Start Taq
Taq Hot Start DNA Polymerase
Taq-star enzyme
Taq-star DNA polymerase
TaqStar
Hot-Start Taq DNA polymerase</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://www.neb.com/products/m0495-hot-start-taq-dna-polymerase</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor.
Definition source: https://www.neb.com/products/m0495-hot-start-taq-dna-polymerase</rdfs:comment>
<rdfs:label xml:lang="en">Hot Start Taq DNA Polymerase</rdfs:label>
<rdfs:seeAlso xml:lang="en">HotStarTaq DNA Polymerase
HotStarTaq
Hot Start DNA Polymerase
Hot Start Taq
Taq Hot Start DNA Polymerase
Taq-star enzyme
Taq-star DNA polymerase
TaqStar
Hot-Start Taq DNA polymerase</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#HousingConditionProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#HousingConditionProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">Housing condition protocol is a protocol that defines instructions about the environmental conditions and handling of experimental organims.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Housing condition protocol is a protocol that defines instructions about the environmental conditions and handling of experimental organims.</rdfs:comment>
<rdfs:label xml:lang="en">housing condition protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#InfectionProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#InfectionProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">Infection protocol is a protocol used in parasitology experiments and defines instructions on how carry out the infection of a organism that acts as host.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Infection protocol is a protocol used in parasitology experiments and defines instructions on how carry out the infection of a organism that acts as host.</rdfs:comment>
<rdfs:label xml:lang="en">infection protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#IntroductionSection -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#IntroductionSection">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000314"/>
<obo:IAO_0000115 xml:lang="en">Introduction section is part of a document. In the context of a protocol, this section show to reader about essential components of the protocol. So, the scientific community could make a decision as to the suitability of the protocol to their experimental problem.This section must include: applications of the protocol, comparison with other protocols, development of the protocol, limitations and purpose.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Introduction section is part of a document. In the context of a protocol, this section show to reader about essential components of the protocol. So, the scientific community could make a decision as to the suitability of the protocol to their experimental problem.This section must include: applications of the protocol, comparison with other protocols, development of the protocol, limitations and purpose.</rdfs:comment>
<rdfs:label xml:lang="en">introduction section</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Kit -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Kit">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<obo:IAO_0000112 xml:lang="en">Universal Linker Kit, laboratory kit that allows customization of protein microarrays.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A set of chemical substances, articles or equipment needed for a specific purpose.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">A set of chemical substances, articles or equipment needed for a specific purpose.
Example of usage:
Universal Linker Kit, laboratory kit that allows customization of protein microarrays.</rdfs:comment>
<rdfs:label xml:lang="en">kit</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#KitName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#KitName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<obo:IAO_0000112 xml:lang="en">Spectrum Plant Total RNA Kit, sufficient for 50 purifications.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Kit name is a textual entity. Kit name is the name of a kit.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Kit name is a textual entity. Kit name is the name of a kit.
Example of usage: Spectrum Plant Total RNA Kit, sufficient for 50 purifications.</rdfs:comment>
<rdfs:label xml:lang="en">kit name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#KlenTaqDNAPolymerase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#KlenTaqDNAPolymerase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#TaqPolymerase"/>
<obo:IAO_0000115 xml:lang="en">KlenTaq DNA Polymerase is missing the N-terminal portion of the gene, encoding Thermus aquaticus (Taq) DNA polymerase, leaving a highly active and even more thermal stable DNA polymerase activity. The missing N-terminal portion is homologous to the 5’→3’ exonuclease region of E. coli. DNA polymerase I.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Cesium Klentaq
Klentaq LA
Klentaq
Omni Klentaq
Omniklentaq
Omni Klentaq LA
Omniklentaq-LA</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://www.klentaq.com/products/klentaq</obo:IAO_0000119>
<rdfs:comment xml:lang="en">KlenTaq DNA Polymerase is missing the N-terminal portion of the gene, encoding Thermus aquaticus (Taq) DNA polymerase, leaving a highly active and even more thermal stable DNA polymerase activity. The missing N-terminal portion is homologous to the 5’→3’ exonuclease region of E. coli. DNA polymerase I.
Definition source: http://www.klentaq.com/products/klentaq</rdfs:comment>
<rdfs:label xml:lang="en">KlenTaq DNA Polymerase</rdfs:label>
<rdfs:seeAlso xml:lang="en">Cesium Klentaq
Klentaq LA
Klentaq
Omni Klentaq
Omniklentaq
Omni Klentaq LA
Omniklentaq-LA</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LimitationOfTheProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#LimitationOfTheProtocol">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PrimerSequence"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PurposeOfProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<owl:disjointWith rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">A major problem faced both in this and other safflower transformation studies is the hyperhydration of transgenic shoots which result in the loss of a large proportion of transgenic shoots. Belide Srinivas., 2011</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Limitation of the protocol is a subclass of textual entity where the possible limitations of the protocol should be discussed. It should be made clear in which situations the Protocol would be unreliable or otherwise unsuccessful.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://www.nature.com/nprot/info/gta.html</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Limitation of the protocol is a subclass of textual entity where the possible limitations of the protocol should be discussed. It should be made clear in which situations the Protocol would be unreliable or otherwise unsuccessful.
Definition source: http://www.nature.com/nprot/info/gta.html
Example of usage: A major problem faced both in this and other safflower transformation studies is the hyperhydration of transgenic shoots which result in the loss of a large proportion of transgenic shoots. Belide Srinivas., 2011</rdfs:comment>
<rdfs:label xml:lang="en">limitation of the protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedGroundTissue -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#LyophilizedGroundTissue">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LyophilizedTissue"/>
<obo:IAO_0000118 xml:lang="en">freeze-dried ground tissue
freeze dried ground tissue</obo:IAO_0000118>
<rdfs:label xml:lang="en">lyophilized ground tissue</rdfs:label>
<rdfs:seeAlso xml:lang="en">freeze-dried ground tissue
freeze dried ground tissue</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedLeaf -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#LyophilizedLeaf">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LyophilizedTissue"/>
<obo:IAO_0000118 xml:lang="en">freeze-dried leaf
freeze dried leaf
lyophilized leaf tissue</obo:IAO_0000118>
<rdfs:label xml:lang="en">lyophilized leaf</rdfs:label>
<rdfs:seeAlso xml:lang="en">freeze-dried leaf
freeze dried leaf
lyophilized leaf tissue</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedRoot -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#LyophilizedRoot">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LyophilizedTissue"/>
<obo:IAO_0000118 xml:lang="en">freeze-dried root
freeze dried root
lyophilized root tissue</obo:IAO_0000118>
<rdfs:label xml:lang="en">lyophilized root</rdfs:label>
<rdfs:seeAlso xml:lang="en">freeze-dried root
freeze dried root
lyophilized root tissue</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedSeed -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#LyophilizedSeed">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LyophilizedTissue"/>
<obo:IAO_0000118 xml:lang="en">freeze-dried seed
freeze dried seed
lyophilized seed tissue</obo:IAO_0000118>
<rdfs:label xml:lang="en">lyophilized seed</rdfs:label>
<rdfs:seeAlso xml:lang="en">freeze-dried seed
freeze dried seed
lyophilized seed tissue</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedTissue -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#LyophilizedTissue">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C12801"/>
<obo:IAO_0000112 xml:lang="en">lyophilized leaf, lyophilized root, lyophilized seed</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">lyophilized tissue is a tissue dried by freezing in a high vacuum</obo:IAO_0000115>
<rdfs:comment xml:lang="en">lyophilized tissue is a tissue dried by freezing in a high vacuum
Example of usage: lyophilized leaf, lyophilized root, lyophilized seed</rdfs:comment>
<rdfs:label xml:lang="en">lyophilized tissue</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LysisBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#LysisBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#buffer"/>
<obo:IAO_0000115 xml:lang="en">A lysis buffer is a buffer solution used for the purpose of lysing cells for use in molecular biology experiments that analyze the compounds of the cells (e.g. western blot). Most lysis buffers contain salts (e.g. Tris-HCl or EDTA) to regulate the acidity and osmolarity of the lysate, while detergents (such as Triton X-100 or SDS) are added to break up membrane structures. In studies like DNA fingerprinting the lysis buffer is used for DNA isolation. Dish soap can be used in a pinch to break down the cell and nuclear membranes, allowing the DNA to be released.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">extraction buffer
isolation buffer
lysis solution
lysate solution
digestion buffer
homogenization buffer
extraction solution
solubilizing buffer
Digestion buffer
Extraction buffer
Extraction solution
Homogenization buffer
Isolation buffer
Lysate solution
Lysis buffer
Lysis solution
tissue lysis buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Lysis_buffer</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A lysis buffer is a buffer solution used for the purpose of lysing cells for use in molecular biology experiments that analyze the compounds of the cells (e.g. western blot). Most lysis buffers contain salts (e.g. Tris-HCl or EDTA) to regulate the acidity and osmolarity of the lysate, while detergents (such as Triton X-100 or SDS) are added to break up membrane structures. In studies like DNA fingerprinting the lysis buffer is used for DNA isolation. Dish soap can be used in a pinch to break down the cell and nuclear membranes, allowing the DNA to be released.
Definition source: https://en.wikipedia.org/wiki/Lysis_buffer</rdfs:comment>
<rdfs:label xml:lang="en">lysis buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">extraction buffer
isolation buffer
lysis solution
lysate solution
digestion buffer
homogenization buffer
extraction solution
solubilizing buffer
Digestion buffer
Extraction buffer
Extraction solution
Homogenization buffer
Isolation buffer
Lysate solution
Lysis buffer
Lysis solution
tissue lysis buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#M-MLVReverseTranscriptase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#M-MLVReverseTranscriptase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ReverseTranscriptase"/>
<obo:IAO_0000115 xml:lang="en">M-MLV reverse transcriptaseis an enzyme from the Moloney murine leukemia virus is a single 75 kDa monomer.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase
Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase
Moloney Murine Leukemia Virus Reverse Transcriptase
M-MuLV Reverse Transcriptase
MMuLV Reverse Transcriptase
MMLV Reverse Transcriptase
MMLV RT
M-MLV RT
MMuLV
M-MuLV RT</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://link.springer.com/article/10.1007%2Fs10529-012-0904-9</obo:IAO_0000119>
<rdfs:comment xml:lang="en">M-MLV reverse transcriptaseis an enzyme from the Moloney murine leukemia virus is a single 75 kDa monomer.
Definition source: http://link.springer.com/article/10.1007%2Fs10529-012-0904-9</rdfs:comment>
<rdfs:label xml:lang="en">M-MLV reverse transcriptase</rdfs:label>
<rdfs:seeAlso xml:lang="en">Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase
Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase
Moloney Murine Leukemia Virus Reverse Transcriptase
M-MuLV Reverse Transcriptase
MMuLV Reverse Transcriptase
MMLV Reverse Transcriptase
MMLV RT
M-MLV RT
MMuLV
M-MuLV RT</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#MPDBBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#MPDBBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000112 xml:lang="en">DNA purification</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">MPDB buffer is a lysis buffer used during isolation of DNA.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">MPDB Buffer
2-methyl-2,4-pentanediol buffer
MPDB
MPDB-buffer</obo:IAO_0000118>
<rdfs:comment xml:lang="en">MPDB buffer is a lysis buffer used during isolation of DNA.
Example of usage: DNA purification</rdfs:comment>
<rdfs:label xml:lang="en">MPDB buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">MPDB Buffer
2-methyl-2,4-pentanediol buffer
MPDB
MPDB-buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Manufacturer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Manufacturer">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ManufacturerName"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000112 xml:lang="en">Bio-Rad, Nalgene, Thermoscientific, Eppendorf, Falcon.</obo:IAO_0000112>
<rdfs:comment xml:lang="en">Manufacturer is a subclass of material entity. Manufacturer is a person, enterprise, or entity that produces equipments, reagents, primers or other laboratory supplies.
Example of usage:
Bio-Rad, Nalgene, Thermoscientific, Eppendorf, Falcon.</rdfs:comment>
<rdfs:label xml:lang="en">manufacturer</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ManufacturerName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ManufacturerName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<obo:IAO_0000115 xml:lang="en">Manufacturer name is a subclass of textual entity. Manufacturer name is the name of a person, enterprise, or entity that produces equipments, reagents, primers or other laboratory supplies.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Manufacturer name is a subclass of textual entity. Manufacturer name is the name of a person, enterprise, or entity that produces equipments, reagents, primers or other laboratory supplies.</rdfs:comment>
<rdfs:label xml:lang="en">manufacturer name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#MaterialsSection -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#MaterialsSection">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000314"/>
<obo:IAO_0000115 xml:lang="en">Materials section is part of a document. In the context of a protocol, this section should list the sample, buffers, reagents, consumables, equipments, kits, software etc., necessary for carrying out your technique.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Materials section is part of a document. In the context of a protocol, this section should list the sample, buffers, reagents, consumables, equipments, kits, software etc., necessary for carrying out your technique.</rdfs:comment>
<rdfs:label xml:lang="en">materials section</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#MechanicalMilling -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#MechanicalMilling">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">mechanical milling is a plant and animal tissue homogenizer used for applications involving sample preparation for DNA, RNA, and protein extractions.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">mechanical mill</obo:IAO_0000118>
<rdfs:comment xml:lang="en">mechanical milling is a plant and animal tissue homogenizer used for applications involving sample preparation for DNA, RNA, and protein extractions.</rdfs:comment>
<rdfs:label xml:lang="en">mechanical milling</rdfs:label>
<rdfs:seeAlso xml:lang="en">mechanical mill</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#MembraneExtractionBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#MembraneExtractionBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:allValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SubcellularProteinFractionationKitforTissues"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000112 xml:lang="en">Protein purification</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">The membrane extraction buffer is part of the Subcellular Protein Fractionation Kit for Tissues. Membrane extraction buffer is a buffer used for membrane protein solubilization.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">MEB
MEB buffer
Membrane Extraction Buffer
MEB extraction buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://www.embl.de/pepcore/pepcore_services/protein_purification/extraction_clarification/solubilisation_membrane_proteins/</obo:IAO_0000119>
<rdfs:comment xml:lang="en">The membrane extraction buffer is part of the Subcellular Protein Fractionation Kit for Tissues. Membrane extraction buffer is a buffer used for membrane protein solubilization.
Definition source:
https://www.embl.de/pepcore/pepcore_services/protein_purification/extraction_clarification/solubilisation_membrane_proteins/
Example of usage: Protein purification</rdfs:comment>
<rdfs:label xml:lang="en">membrane extraction buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">MEB
MEB buffer
Membrane Extraction Buffer
MEB extraction buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#MethodsSection -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#MethodsSection">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000314"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">Methods section is part of a document. This is the section where the protocol is presented.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Methods section is part of a document. This is the section where the protocol is presented.</rdfs:comment>
<rdfs:label xml:lang="en">methods section</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#MicrocentrifugeTube -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#MicrocentrifugeTube">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000112 xml:lang="en">Pour off supernatant into 1.5 ml microfuge tube.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Microcentrifuge tube is a supply. Microcentrifuge tubes are plastic tubes with capacities from 250 ul to 2.0 ml.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Microcentrifuge tips
microfuge tube</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://en.wikipedia.org/wiki/Laboratory_centrifuge#Centrifuge_tubes</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Microcentrifuge tube is a supply. Microcentrifuge tubes are plastic tubes with capacities from 250 ul to 2.0 ml.
Definition source: http://en.wikipedia.org/wiki/Laboratory_centrifuge#Centrifuge_tubes
Example of usage: Pour off supernatant into 1.5 ml microfuge tube.</rdfs:comment>
<rdfs:label xml:lang="en">microcentrifuge tube</rdfs:label>
<rdfs:seeAlso xml:lang="en">Microcentrifuge tips
microfuge tube</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Micropestle -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Micropestle">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">micropestle is a very small pestle used for tissue homogenization. Some micropestles have an end which fits 1.5mL microcentrifuge tube; others micropestles have an end which fits 2ml microcentrifuge tube.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">micropestle is a very small pestle used for tissue homogenization. Some micropestles have an end which fits 1.5mL microcentrifuge tube; others micropestles have an end which fits 2ml microcentrifuge tube.</rdfs:comment>
<rdfs:label xml:lang="en">micropestle</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#MixerMill -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#MixerMill">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">Mixer mill is a machine used for grinding dry, brittle samples.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Mixer/Mill
MixerMill
shaker mill
high-energy ball mill</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Mixer mill is a machine used for grinding dry, brittle samples.</rdfs:comment>
<rdfs:label xml:lang="en">mixer mill</rdfs:label>
<rdfs:seeAlso xml:lang="en">Mixer/Mill
MixerMill
shaker mill
high-energy ball mill</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#MortarAndPestle -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#MortarAndPestle">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">A mortar and pestle is a device used to crushing and grinding tissue into a fine powder.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">mortar grinding machine
mortar grinder
pestle and mortar</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Mortar_and_pestle</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A mortar and pestle is a device used to crushing and grinding tissue into a fine powder.
Definition source: https://en.wikipedia.org/wiki/Mortar_and_pestle</rdfs:comment>
<rdfs:label xml:lang="en">mortar and pestle</rdfs:label>
<rdfs:seeAlso xml:lang="en">mortar grinding machine
mortar grinder
pestle and mortar</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#N-PhenacylthiazoliumBromide -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#N-PhenacylthiazoliumBromide">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PhenacylBromideReagent"/>
<obo:IAO_0000112 xml:lang="en">N-phenacylthiazolium bromide (PTB) is used during DNA extraction. This chemical compound has demonstrated to cleave Maillard products, thus freeing up DNA for PCR amplification.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">N-phenacylthiazolium bromide is a compound that breaks cross-links in Advanced glycation end products.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">N-phenacyl thiazolium bromide
N-Phenacylthiazolium Bromide
N-Phenacylthiazolium bromide
PTB</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Phenacyl</obo:IAO_0000119>
<rdfs:comment xml:lang="en">N-phenacylthiazolium bromide is a compound that breaks cross-links in Advanced glycation end products.
Definition source: https://en.wikipedia.org/wiki/Phenacyl
Example of usage:
N-phenacylthiazolium bromide (PTB) is used during DNA extraction. This chemical compound has demonstrated to cleave Maillard products, thus freeing up DNA for PCR amplification.</rdfs:comment>
<rdfs:label xml:lang="en">N-phenacylthiazolium bromide</rdfs:label>
<rdfs:seeAlso xml:lang="en">N-phenacyl thiazolium bromide
N-Phenacylthiazolium Bromide
N-Phenacylthiazolium bromide
PTB</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#NEBPlusMNase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#NEBPlusMNase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:allValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SubcellularProteinFractionationKitforTissues"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">The nuclear extraction buffer plus MNase is part of the Subcellular Protein Fractionation Kit for Tissues. Nuclear extraction buffer with micrococcal nuclease releases chromatin-bound nuclear proteins.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">nuclear extraction buffer with micrococcal nuclease
NEB + MNase
nuclear extraction buffer plus micrococcal nuclease (chromatin-bound fraction)</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://www.thermofisher.com/es/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/subcellular-protein-fractionation.html</obo:IAO_0000119>
<rdfs:comment xml:lang="en">The nuclear extraction buffer plus MNase is part of the Subcellular Protein Fractionation Kit for Tissues. Nuclear extraction buffer with micrococcal nuclease releases chromatin-bound nuclear proteins.
Definition source: https://www.thermofisher.com/es/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/subcellular-protein-fractionation.html</rdfs:comment>
<rdfs:label xml:lang="en">nuclear extraction buffer plus MNase</rdfs:label>
<rdfs:seeAlso xml:lang="en">nuclear extraction buffer with micrococcal nuclease
NEB + MNase
nuclear extraction buffer plus micrococcal nuclease (chromatin-bound fraction)</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#NH4Buffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#NH4Buffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PCRBuffer"/>
<obo:IAO_0000115 xml:lang="en">NH4 Buffer is an ready-to-use buffer for PCR using Taq DNA Polymerases (both recombinant and native).</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Taq Buffer with (NH4)2SO4
NH4 Buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">PERSON:Olga Giraldo</obo:IAO_0000119>
<rdfs:comment xml:lang="en">NH4 Buffer is an ready-to-use buffer for PCR using Taq DNA Polymerases (both recombinant and native).</rdfs:comment>
<rdfs:label xml:lang="en">NH4 buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">Taq Buffer with (NH4)2SO4
NH4 Buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Neurotoxic -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Neurotoxic">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122"/>
<obo:IAO_0000112 xml:lang="en">avoid contact with skin, eyes or mucous membranes; keep away from sources of ignition. When handling , wear gloves. Reference: Nature Protocols</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Poisonous to nerves or nerve cells.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://wordnetweb.princeton.edu/perl/webwn?s=neurotoxic</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Poisonous to nerves or nerve cells.
Definition source: http://wordnetweb.princeton.edu/perl/webwn?s=neurotoxic
Example of usage: avoid contact with skin, eyes or mucous membranes; keep away from sources of ignition. When handling , wear gloves.</rdfs:comment>
<rdfs:label xml:lang="en">neurotoxic</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#NuclearExtractionBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#NuclearExtractionBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:allValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SubcellularProteinFractionationKitforTissues"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">The nuclear extraction buffer is part of the Subcellular Protein Fractionation Kit for Tissues.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">The nuclear extraction buffer is part of the Subcellular Protein Fractionation Kit for Tissues.</rdfs:comment>
<rdfs:label xml:lang="en">nuclear extraction buffer</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#NucleicAcidExtractionProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#NucleicAcidExtractionProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ProteinExtractionProtocol"/>
<obo:IAO_0000115 xml:lang="en">Nucleic acid extraction protocol is a subclass of laboratory protocol. A nucleic acid extraction protocol is a step by step description of a procedure used to recover the nucleic acid fraction of an input material entity.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Nucleic acid extraction protocol is a subclass of laboratory protocol. A nucleic acid extraction protocol is a step by step description of a procedure used to recover the nucleic acid fraction of an input material entity.</rdfs:comment>
<rdfs:label xml:lang="en">nucleic acid extraction protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#OligodTprimer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#OligodTprimer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">oligo (dT) primer is a short sequence of deoxy-thymine nucleotides. Oligo (dT) is tagged as a complementary primer which binds to the poly-A tail providing a free 3'-OH end that can be extended by reverse transcriptase to create the complementary DNA strand.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Oligo dT primer
Oligo dT primers
oligo dT primer
oligo(dT) primer
oligo(dT) primers
Oligo(dT) primer
oligo (dT) primers</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/CDNA_library</obo:IAO_0000119>
<rdfs:comment xml:lang="en">oligo (dT) primer is a short sequence of deoxy-thymine nucleotides. Oligo (dT) is tagged as a complementary primer which binds to the poly-A tail providing a free 3'-OH end that can be extended by reverse transcriptase to create the complementary DNA strand.
Definition source: https://en.wikipedia.org/wiki/CDNA_library</rdfs:comment>
<rdfs:label xml:lang="en">oligo (dT) primer</rdfs:label>
<rdfs:seeAlso xml:lang="en">Oligo dT primer
Oligo dT primers
oligo dT primer
oligo(dT) primer
oligo(dT) primers
Oligo(dT) primer
oligo (dT) primers</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#OutcomeOfTheProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#OutcomeOfTheProtocol">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<obo:IAO_0000115 xml:lang="en">protocol outcome is a textual entity that is an outcome or expected result by a protocol execution.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">protocol outcome is a textual entity that is an outcome or expected result by a protocol execution.</rdfs:comment>
<rdfs:label xml:lang="en">outcome of the protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PCRAmplification -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PCRAmplification">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolInstruction"/>
<rdfs:comment xml:lang="en">PCR amplification is used in molecular biology to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It is a way to amplify a focused segment of DNA, useful for such purposes as the diagnosis and monitoring of genetic diseases, identification of criminals (in the field of forensics), and studying the function of a targeted segment of DNA.
Definition source: http://learn.genetics.utah.edu/content/labs/pcr/</rdfs:comment>
<rdfs:label xml:lang="en">PCR amplification</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PCRBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PCRBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#buffer"/>
<obo:IAO_0000112 xml:lang="en">Taqman buffer, PCR Buffer with (or without) MgCl2</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">PCR buffer is a solution that provide a suitable chemical environment for optimum activity and stability of the DNA polymerase</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">PCR Buffer
PCR enzyme buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Polymerase_chain_reaction</obo:IAO_0000119>
<rdfs:comment xml:lang="en">PCR buffer is a solution that provide a suitable chemical environment for optimum activity and stability of the DNA polymerase.
Definition source: https://en.wikipedia.org/wiki/Polymerase_chain_reaction
Example of usage: Taqman buffer, PCR Buffer with (or without) MgCl2</rdfs:comment>
<rdfs:label xml:lang="en">PCR buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">PCR Buffer
PCR enzyme buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PausePoint -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PausePoint">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">PAUSE POINT: Can be left overnight at 48 C or frozen for up to a month at -20 C.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Pause point is the point were the protocol naturally breaks into separate stages.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Pause point is the point were the protocol naturally breaks into separate stages.
Example of usage:
PAUSE POINT: Can be left overnight at 48 C or frozen for up to a month at -20 C.</rdfs:comment>
<rdfs:label xml:lang="en">pause point</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PelletExtractionBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PelletExtractionBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:allValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#SubcellularProteinFractionationKitforTissues"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">The pellet extraction buffer is part of the Subcellular Protein Fractionation Kit for Tissues. Pellet extraction buffer is used to isolate cytoskeletal proteins.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">PEB
PEB buffer</obo:IAO_0000118>
<rdfs:comment xml:lang="en">The pellet extraction buffer is part of the Subcellular Protein Fractionation Kit for Tissues. Pellet extraction buffer is used to isolate cytoskeletal proteins.</rdfs:comment>
<rdfs:label xml:lang="en">pellet extraction buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">PEB
PEB buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PhenacylBromideReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PhenacylBromideReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">Phenacyl bromide reagent is a reagent that has formula C8H7BrO.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">2-bromo-1-phenylethanone
omega-Bromoacetophenone
alpha-Bromoacetophenone
Bromomethyl phenyl ketone
2-Bromoacetophenone</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">phenacyl bromide reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PhenolChloroformIsoamylAlcohol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PhenolChloroformIsoamylAlcohol">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830"/>
<obo:IAO_0000115 xml:lang="en">A mixture consisting of equal parts of equilibrated phenol and chloroform:isoamyl alcohol (24:1) is frequently used to remove proteins from preparations of nucleic acids. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8282.full?text_only=true</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A mixture consisting of equal parts of equilibrated phenol and chloroform:isoamyl alcohol (24:1) is frequently used to remove proteins from preparations of nucleic acids. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
Definition source: http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8282.full?text_only=true</rdfs:comment>
<rdfs:label xml:lang="en">Phenol:Chloroform:Isoamyl alcohol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PhosphateBufferedSaline -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PhosphateBufferedSaline">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#buffer"/>
<obo:IAO_0000112 xml:lang="en">PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. It is used to rinse containers containing cells. PBS can be used as a diluent in methods to dry biomolecules, as water molecules within it will be structured around the substance (protein, for example) to be ‘dried’ and immobilized to a solid surface. The thin film of water that binds to the substance prevents denaturation or other conformational changes. Carbonate buffers may be used for the same purpose but with less effectiveness. PBS can be used to take a reference spectrum when measuring the protein adsorption in ellipsometry (http://protocolsonline.com/recipes/phosphate-buffered-saline-pbs/)</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Phosphate buffered saline (PBS) is a non toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis. PBS can be used for the extraction of spermatozoa and cells from swabs and PBS does not affect subsequent DNA profiling.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">PBS
Phosphate Buffered Saline
phosphate-buffered saline</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://www.maths.lancs.ac.uk/~lucy/publications/Martin-Pirie-Ford-Callaghan-McTurk-Lucy-Scrimger-2006.pdf</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Phosphate buffered saline (PBS) is a non toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis. PBS can be used for the extraction of spermatozoa and cells from swabs and PBS does not affect subsequent DNA profiling.
Definition source:
http://www.maths.lancs.ac.uk/~lucy/publications/Martin-Pirie-Ford-Callaghan-McTurk-Lucy-Scrimger-2006.pdf</rdfs:comment>
<rdfs:label xml:lang="en">phosphate buffered saline</rdfs:label>
<rdfs:seeAlso xml:lang="en">Example of usage:
PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. It is used to rinse containers containing cells. PBS can be used as a diluent in methods to dry biomolecules, as water molecules within it will be structured around the substance (protein, for example) to be ‘dried’ and immobilized to a solid surface. The thin film of water that binds to the substance prevents denaturation or other conformational changes. Carbonate buffers may be used for the same purpose but with less effectiveness. PBS can be used to take a reference spectrum when measuring the protein adsorption in ellipsometry (http://protocolsonline.com/recipes/phosphate-buffered-saline-pbs/)</rdfs:seeAlso>
<rdfs:seeAlso xml:lang="en">PBS
Phosphate Buffered Saline
phosphate-buffered saline</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PhysicalDisruptionEquipmentAndSupplies -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PhysicalDisruptionEquipmentAndSupplies">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<rdfs:comment xml:lang="en">Equipment used for cell disruption methods.
Some examples: mortar and pestle; handheld device (for example, Dounce and Potter-Elvehjem homogenizers), motorized devices (for example, blenders).</rdfs:comment>
<rdfs:label xml:lang="en">physical disruption equipment and supplies</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PluronicF68 -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PluronicF68">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_53514"/>
<obo:IAO_0000118 xml:lang="en">Pluronic F-68
Pluronic F68</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Pluronic F68 is used in cell culture as a stabilizer of cell membranes protecting from membrane shearing and additionally acts as an anti-foaming agent.
Definition source:
http://bit.do/thermofisher-PluronicF68</rdfs:comment>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">pluronic F-68</rdfs:label>
<rdfs:seeAlso xml:lang="en">Pluronic F-68
Pluronic F68</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PolimeraseChainReactionProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PolimeraseChainReactionProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#EnzimaticAmplificationProtocol"/>
<obo:IAO_0000115 xml:lang="en">PCR protocol is a step by step description of the procedure to amplify a particular DNA sequence.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">PCR protocol is a step by step description of the procedure to amplify a particular DNA sequence.</rdfs:comment>
<rdfs:label xml:lang="en">polimerase chain reaction protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PolyEthyleneGlycolReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PolyEthyleneGlycolReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000112 xml:lang="en">precipitation of DNA</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">poly ethylene glycol is a polymer composed of repeating ethyleneoxy units.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">polyethylene oxide
PEO
alpha,omega-hydroxypoly(ethylene oxide)
Macrogol
alpha-hydro-omega-hydroxypoly(oxy-1,2-ethanediyl)
Polyethylene glycol
1,2-ethanediol homopolymer
Polyaethylenglykole
Glycols, polyethylene
polyoxyethylene
polyethylene glycols
ethylene glycol homopolymer
(C2H4O)n
poly(ethyleneoxide)
polyethylene glycol
alpha-hydro-omega-hydroxypoly(oxyethylene)
Polyaethylenglykol
poly(oxyethylene)
PEG
POE
Poly(ethylene glycol)</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:comment xml:lang="en">example of usage source: chrome-extension://padcapdkhelngdelppbbjmkmkfceoikg/content/pdf/viewer/viewer.html?file=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC333871%2Fpdf%2Fnar00242-0172.pdf</rdfs:comment>
<rdfs:label xml:lang="en">poly ethylene glycol reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PolytronHomogenizer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PolytronHomogenizer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Homogenizer"/>
<obo:IAO_0000115 xml:lang="en">Polytron homogenizer is a instrument used for pulverizing plant and animal tissue, grain and seed, soil extraction, DNA/RNA extraction.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">polytron
poly-tron mixer
polytron mixer</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Polytron homogenizer is a instrument used for pulverizing plant and animal tissue, grain and seed, soil extraction, DNA/RNA extraction.</rdfs:comment>
<rdfs:label xml:lang="en">polytron homogenizer</rdfs:label>
<rdfs:seeAlso xml:lang="en">polytron
poly-tron mixer
polytron mixer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Polyvinylpyrrolidone -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Polyvinylpyrrolidone">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#VinylPolymerMacromoleculeReagent"/>
<obo:IAO_0000112 xml:lang="en">PVP (polyvinylpyrrolidone) is added to remove phenolic compounds from plant DNA extracts. PVP precipitates polyphenolics.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A vinyl polymer composed of repeating -CH2-CR- units where R is a 2-oxopyrrolidin-1-yl group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">poly(vinylpyrrolidone)
poly[1-(2-oxopyrrolidin-1-yl)ethylene]
poly(vinylpyrrolidone)
N-Vinylbutyrolactam polymer
Vinylpyrrolidone polymer
Poly(N-vinyl-2-pyrrolidone) N-Vinylpyrrolidinone polymer Poly(1-vinyl-2-pyrrolidinone)
Polividone
polyvidone
Poly(N-vinyl-2-pyrrolidinone)
1-Vinyl-2-pyrrolidone polymer
1-Ethenyl-2-pyrrolidinone homopolymer
Poly-N-vinyl pyrrolidone; Poly(1-ethenyl-2-pyrrolidinone) Poly(1-vinylpyrrolidinone); Poly(N-vinylbutyrolactam) Poly(1-vinyl-2-pyrrolidinone) homopolymer
PVPON
PVP
PVP40
PVP-40
PVP 40
PVP10
PVP-10
PVP 10
poly(N-vinyl pyrrolidone)
Crospovidonum
Polyvinylpyrrolidone
Povidone
Polyvidonum
Poly(N-vinylpyrrolidinone)
1-Vinyl-2-pyrrolidinone homopolymer
Poly(1-vinyl-2-pyrrolidone)
Polividona
polyvinylpyrrolidone
N-Vinyl-2-pyrrolidone polymer
N-Vinylpyrrolidone polymer
Vinylpyrrolidinone polymer
poly(N-vinylpyrrolidone)
1-Ethenyl-2-pyrrolidinone polymers
1-Vinyl-2-pyrrolidinone polymer
(C6H9NO)n
Poly(1-(2-oxo-1-pyrrolidinyl)ethylene)</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A vinyl polymer composed of repeating -CH2-CR- units where R is a 2-oxopyrrolidin-1-yl group.
Definition source: CHEBI
Example of usage:
PVP (polyvinylpyrrolidone) is added to remove phenolic compounds from plant DNA extracts. PVP precipitates polyphenolics.</rdfs:comment>
<rdfs:label xml:lang="en">polyvinylpyrrolidone</rdfs:label>
<rdfs:seeAlso xml:lang="en">poly(vinylpyrrolidone)
poly[1-(2-oxopyrrolidin-1-yl)ethylene]
poly(vinylpyrrolidone)
N-Vinylbutyrolactam polymer
Vinylpyrrolidone polymer
Poly(N-vinyl-2-pyrrolidone) N-Vinylpyrrolidinone polymer Poly(1-vinyl-2-pyrrolidinone)
Polividone
polyvidone
Poly(N-vinyl-2-pyrrolidinone)
1-Vinyl-2-pyrrolidone polymer
1-Ethenyl-2-pyrrolidinone homopolymer
Poly-N-vinyl pyrrolidone; Poly(1-ethenyl-2-pyrrolidinone) Poly(1-vinylpyrrolidinone); Poly(N-vinylbutyrolactam) Poly(1-vinyl-2-pyrrolidinone) homopolymer
PVPON
PVP
PVP40
PVP-40
PVP 40
PVP10
PVP-10
PVP 10
poly(N-vinyl pyrrolidone)
Crospovidonum
Polyvinylpyrrolidone
Povidone
Polyvidonum
Poly(N-vinylpyrrolidinone)
1-Vinyl-2-pyrrolidinone homopolymer
Poly(1-vinyl-2-pyrrolidone)
Polividona
polyvinylpyrrolidone
N-Vinyl-2-pyrrolidone polymer
N-Vinylpyrrolidone polymer
Vinylpyrrolidinone polymer
poly(N-vinylpyrrolidone)
1-Ethenyl-2-pyrrolidinone polymers
1-Vinyl-2-pyrrolidinone polymer
(C6H9NO)n
Poly(1-(2-oxo-1-pyrrolidinyl)ethylene)</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PortableIceChest -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PortableIceChest">
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<obo:IAO_0000112 xml:lang="en">Place bags in a portable ice chest or other container with ice to keep samples cool.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A portable ice chest is a supply. A cooler, cool box, portable ice chest, chilly bin (in New Zealand), or esky (Australia) most commonly is an insulated box used to keep the things cool.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">cooler
cool box
chilly bin
esky</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://en.wikipedia.org/wiki/Cooler</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A portable ice chest is a supply. A cooler, cool box, portable ice chest, chilly bin (in New Zealand), or esky (Australia) most commonly is an insulated box used to keep the things cool.
Definition source: http://en.wikipedia.org/wiki/Cooler
Example of usage:
Place bags in a portable ice chest or other container with ice to keep samples cool.</rdfs:comment>
<rdfs:label xml:lang="en">portable ice chest</rdfs:label>
<rdfs:seeAlso xml:lang="en">cooler
cool box
chilly bin
esky</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PotassiumSaltReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PotassiumSaltReagent">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#AlkaliMetalSaltReagent"/>
<obo:IAO_0000115 xml:lang="en">Any alkali metal salt having potassium(1+) as the cation.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">potassium salts
Kaliumsalze
Kaliumsalz</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">potassium salt reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PotterElvehjemHomogenizer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PotterElvehjemHomogenizer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Homogenizer"/>
<obo:IAO_0000115 xml:lang="en">Shearing is caused by a PTFE- coated pestle that is manually or mechanically driven into a conical vessel</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Shearing is caused by a PTFE- coated pestle that is manually or mechanically driven into a conical vessel</rdfs:comment>
<rdfs:label xml:lang="en">potter-elvehjem homogenizer</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PowderedLeaf -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PowderedLeaf">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PowderedTissue"/>
<obo:IAO_0000118 xml:lang="en">pulverized leaf
pulverized leaf tissue
powdered leaf tissue</obo:IAO_0000118>
<rdfs:label xml:lang="en">powdered leaf</rdfs:label>
<rdfs:seeAlso xml:lang="en">pulverized leaf
pulverized leaf tissue
powdered leaf tissue</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PowderedRoot -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PowderedRoot">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PowderedTissue"/>
<obo:IAO_0000118 xml:lang="en">pulverized root
pulverized root tissue
powdered root tissue</obo:IAO_0000118>
<rdfs:label xml:lang="en">powdered root</rdfs:label>
<rdfs:seeAlso xml:lang="en">pulverized root
pulverized root tissue
powdered root tissue</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PowderedSeed -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PowderedSeed">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PowderedTissue"/>
<obo:IAO_0000118 xml:lang="en">pulverized seed
pulverized seed tissue
powdered seed tissue</obo:IAO_0000118>
<rdfs:label xml:lang="en">powdered seed</rdfs:label>
<rdfs:seeAlso xml:lang="en">pulverized seed
pulverized seed tissue
powdered seed tissue</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PowderedTissue -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PowderedTissue">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C12801"/>
<obo:IAO_0000112 xml:lang="en">Grind the seed to a powder. Transfer the powdered seed to a container and weigh the specified amount.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Powdered tissue is a material entity. Powdered tissue is a tissue that has been pulverirized.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">pulverized tissue</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Powdered tissue is a material entity. Powdered tissue is a tissue that has been pulverirized.
Example of usage:
Grind the seed to a powder. Transfer the powdered seed to a container and weigh the specified amount.</rdfs:comment>
<rdfs:label xml:lang="en">powdered tissue</rdfs:label>
<rdfs:seeAlso xml:lang="en">pulverized tissue</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PrecipitationReaction -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PrecipitationReaction">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolInstruction"/>
<obo:IAO_0000115 xml:lang="en">precipitation reaction, is the third basic step in a DNA extraction. This is commonly achieved by ice-cold ethanol, or isopropanol, or phenol-chloroform, or by using minicolumn.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">precipitation reaction, is the third basic step in a DNA extraction. This is commonly achieved by ice-cold ethanol, or isopropanol, or phenol-chloroform, or by using minicolumn.</rdfs:comment>
<rdfs:label xml:lang="en">precipitation reaction</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Primer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Primer">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/ERO_0001203"/>
<obo:IAO_0000112 xml:lang="en">Oligos used in PCR.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A primer is a short strand of nucleic acid that serves as a starting point for DNA or RNA synthesis.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Primer
primers
Primers</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://en.wikipedia.org/wiki/Primer_%28molecular_biology%29</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A primer is a short strand of nucleic acid that serves as a starting point for DNA or RNA synthesis.
Definition source: http://en.wikipedia.org/wiki/Primer_%28molecular_biology%29
Example of usage: Oligos used in PCR.</rdfs:comment>
<rdfs:label xml:lang="en">primer</rdfs:label>
<rdfs:seeAlso xml:lang="en">Primer
primers
Primers</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PrimerName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PrimerName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#SoftwareName"/>
<obo:IAO_0000112 xml:lang="en">CII-fw, CII-rv.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Primer name is a textual entity. Primer name is the name of a primer.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Primer name is a textual entity. Primer name is the name of a primer.
Example of usage: CII-fw, CII-rv.</rdfs:comment>
<rdfs:label xml:lang="en">primer name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PrimerSequence -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PrimerSequence">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PurposeOfProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<owl:disjointWith rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">5′-GAATGATGTACCACCTTTG-3′</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Primer sequence is a textual entity. Primer sequence is a serie ordered of bases coded as letters (A,C,G,T) without spaces, that identify to a particular primer.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Primer sequence is a textual entity. Primer sequence is a serie ordered of bases coded as letters (A,C,G,T) without spaces, that identify to a particular primer.
Example of usage: 5′-GAATGATGTACCACCTTTG-3′</rdfs:comment>
<rdfs:label xml:lang="en">primer sequence</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ProcessedDNA -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ProcessedDNA">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_16991"/>
<obo:IAO_0000115 xml:lang="en">Is a DNA that suffers changes during a laboratory procedure.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Is a DNA that suffers changes during a laboratory procedure.</rdfs:comment>
<rdfs:label xml:lang="en">processed DNA</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ProcessedRNA -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ProcessedRNA">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">Is a RNA that suffers changes during a laboratory procedure.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Is a RNA that suffers changes during a laboratory procedure.</rdfs:comment>
<rdfs:label xml:lang="en">processed RNA</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ProteinExtractionProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ProteinExtractionProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">Protein extraction protocol is a subclass of laboratory protocol. Protein extraction is an extraction where the desired output material is protein.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Protein isolation protocol</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Protein extraction protocol is a subclass of laboratory protocol. Protein extraction is an extraction where the desired output material is protein.</rdfs:comment>
<rdfs:label xml:lang="en">protein extraction protocol</rdfs:label>
<rdfs:seeAlso xml:lang="en">Protein isolation protocol</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ProteinPrecipitationBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ProteinPrecipitationBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#buffer"/>
<rdfs:label xml:lang="en">protein precipitation buffer</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ProtocolIdentifier -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ProtocolIdentifier">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000028"/>
<rdfs:comment xml:lang="en">Any number that identifies a specific experimental protocol</rdfs:comment>
<rdfs:label xml:lang="en">protocol identifier</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ProtocolInstruction -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ProtocolInstruction">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42688"/>
<obo:IAO_0000115 xml:lang="en">A protocol instruction should have sufficient level of detail and quantitative information to communicate it between domain experts, so that different domain experts will reliably be able to independently reproduce the procedure.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">EFO</obo:IAO_0000119>
<rdfs:label xml:lang="en">protocol instruction</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#PurposeOfProtocol"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<owl:disjointWith rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">The extraction procedure precisely followed that of Lisec et al. (2006), which was developed from the protocol of Fiehn et al. (2000a). Allwood J. W., 2009</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Provenance of the protocol is a subclass of textual entity. Provenance of the protocol include references to key papers where the protocol has been used previously. Indicate if your protocol is the result of modify a previous one or if it is the result of reuse steps of several (more than 1) protocols.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Provenance of the protocol is a subclass of textual entity. Provenance of the protocol include references to key papers where the protocol has been used previously. Indicate if your protocol is the result of modify a previous one or if it is the result of reuse steps of several (more than 1) protocols.
Example of usage: The extraction procedure precisely followed that of Lisec et al. (2006), which was developed from the protocol of Fiehn et al. (2000a). Allwood J. W., 2009</rdfs:comment>
<rdfs:label xml:lang="en">provenance of the protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Publication -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Publication">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000030"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<owl:someValuesFrom rdf:resource="http://prismstandard.org/namespaces/basic/2.0/prism:publicationName"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">publication is a magazine, or other publication, in which a resource was/will be published.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">publication is a magazine, or other publication, in which a resource was/will be published.</rdfs:comment>
<rdfs:label xml:lang="en">publication</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PurposeOfProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#PurposeOfProtocol">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<owl:disjointWith rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">Development of a method to isolate small RNAs from different plant species (...) that no need of first total RNA extraction and is not based on the commercially available TRIzol; Reagent or columns. Rosas-Cardenas, F.d.F., et al., A simple and efficient method for isolating small RNAs from different plant species. Plant Methods, 2011. 7(4): p. 7.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">The purpose of a protocol is a subclass of textual entity. Its role, is to enable readers to make a decision as to the suitability of the protocol to their experimental problem.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">The purpose of a protocol is a subclass of textual entity. Its role, is to enable readers to make a decision as to the suitability of the protocol to their experimental problem.
Example of usage:
Development of a method to isolate small RNAs from different plant species (...) that no need of first total RNA extraction and is not based on the commercially available TRIzol; Reagent or columns. Rosas-Cardenas, F.d.F., et al., A simple and efficient method for isolating small RNAs from different plant species. Plant Methods, 2011. 7(4): p. 7.</rdfs:comment>
<rdfs:label xml:lang="en">purpose of the protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Pyrophoric -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Pyrophoric">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122"/>
<obo:IAO_0000112 xml:lang="en">Iron sulfide and many reactive metals including uranium, when powdered or thinly sliced. Pyrophoric materials are often water-reactive as well and will ignite when they contact water or humid air. They can be handled safely in atmospheres of argon or (with a few exceptions) nitrogen. Most pyrophoric fires should be extinguished with a Class D fire extinguisher for burning metals.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A chemical substance that will ignite spontaneously in air at or below a temperature of 130 F (54.4 C).</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://www.utexas.edu/safety/ehs/msds/glossary/?page=p</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A chemical substance that will ignite spontaneously in air at or below a temperature of 130 F (54.4 C).
Definition source: http://www.utexas.edu/safety/ehs/msds/glossary/?page=p
Example of usage: Iron sulfide and many reactive metals including uranium, when powdered or thinly sliced. Pyrophoric materials are often water-reactive as well and will ignite when they contact water or humid air. They can be handled safely in atmospheres of argon or (with a few exceptions) nitrogen. Most pyrophoric fires should be extinguished with a Class D fire extinguisher for burning metals.</rdfs:comment>
<rdfs:label xml:lang="en">pyrophoric</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#QuaternaryAmmoniumSaltReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#QuaternaryAmmoniumSaltReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">Derivatives of ammonium compounds, (NH4(+))Y(-), in which all four of the hydrogens bonded to nitrogen have been replaced with univalent (usually hydrocarbyl) groups.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">quaternary ammonium compounds
quaternary ammonium salt
quaternary ammonium salts
quaternary ammonium compound</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">quaternary ammonium salt reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#RNAExtractionProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#RNAExtractionProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#NucleicAcidExtractionProtocol"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#CellDisruption"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#DenaturationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#PrecipitationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasInstruction"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#WashingNucleicAcids"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">RNA extraction protocol is a subclass of nucleic acid extraction protocol. RNA extraction protocol, is a step by step description of a procedure in which is recovered a fraction of RNA from a material entity.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">RNA isolation protocol</obo:IAO_0000118>
<rdfs:comment xml:lang="en">RNA extraction protocol is a subclass of nucleic acid extraction protocol. RNA extraction protocol, is a step by step description of a procedure in which is recovered a fraction of RNA from a material entity.</rdfs:comment>
<rdfs:label xml:lang="en">RNA extraction protocol</rdfs:label>
<rdfs:seeAlso xml:lang="en">RNA isolation protocol</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#RNASeparatedByMolecularSize -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#RNASeparatedByMolecularSize">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProcessedRNA"/>
<obo:IAO_0000115 xml:lang="en">RNA separated by molecular size is obtained after the aplication of electrical potential to move material through a defined matrix.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">RNA separated by molecular size is obtained after the aplication of electrical potential to move material through a defined matrix.</rdfs:comment>
<rdfs:label xml:lang="en">RNA separated by molecular size</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#RandomPrimer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#RandomPrimer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">Random primers are used for synthesizing large pools of cDNA. They are also ideal for non-polyadenylated RNA, such as bacterial RNA, because they anneal throughout the target molecule. Degraded transcripts such as FFPE samples and secondary structure within the RNA do not pose as big a problem with random primers as they do with gene-specific primers and oligo(dT) primers.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">https://www.thermofisher.com/es/en/home/life-science/pcr/reverse-transcription/rna-priming-strategies.html</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Random primers are used for synthesizing large pools of cDNA. They are also ideal for non-polyadenylated RNA, such as bacterial RNA, because they anneal throughout the target molecule. Degraded transcripts such as FFPE samples and secondary structure within the RNA do not pose as big a problem with random primers as they do with gene-specific primers and oligo(dT) primers.
Definition source: https://www.thermofisher.com/es/en/home/life-science/pcr/reverse-transcription/rna-priming-strategies.html</rdfs:comment>
<rdfs:label xml:lang="en">random primer</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ReagentList -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ReagentList">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000100"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#SoftwareList"/>
<obo:IAO_0000115 xml:lang="en">Reagent list is a data set of the names or identifiers of the reagents (purchased ready-to-use), that are part of materials section of a protocol.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Reagent list is a data set of the names or identifiers of the reagents (purchased ready-to-use), that are part of materials section of a protocol.</rdfs:comment>
<rdfs:label xml:lang="en">reagent list</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ReagentName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ReagentName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#ReagentList"/>
</owl:Restriction>
</rdfs:subClassOf>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#SoftwareName"/>
<obo:IAO_0000112 xml:lang="en">Taq DNA Polymerase from Thermus aquaticus with 10X reaction buffer without MgCl2.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Reagent name is a textual entity. Reagent name is the name of a reagent.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Reagent name is a textual entity. Reagent name is the name of a reagent.
Example of usage: Taq DNA Polymerase from Thermus aquaticus with 10X reaction buffer without MgCl2.</rdfs:comment>
<rdfs:label xml:lang="en">reagent name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#RecipeList -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#RecipeList">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000100"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">recipe list is a data set of the names or identifiers of the recipes, that are part of materials section of a protocol.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">recipe list is a data set of the names or identifiers of the recipes, that are part of materials section of a protocol.</rdfs:comment>
<rdfs:label xml:lang="en">recipe list</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#RecipeName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#RecipeName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<obo:IAO_0000115 xml:lang="en">Recipe name is a textual entity. Recipe name is the name of a solution, buffer, media, etc that is prepared in the laboratory.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Recipe name is a textual entity. Recipe name is the name of a solution, buffer, media, etc that is prepared in the laboratory.</rdfs:comment>
<rdfs:label>recipe name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ReferenceList -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ReferenceList">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000314"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.org/spar/biro/BibliographicReference"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">Reference lists contain a complete list of all the sources (books, journal articles, websites, etc.) that you have cited directly in a document. That means that if there are in-text citations for a source there is a reference list entry, and vice versa.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">list of works cited</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Reference lists contain a complete list of all the sources (books, journal articles, websites, etc.) that you have cited directly in a document. That means that if there are in-text citations for a source there is a reference list entry, and vice versa.</rdfs:comment>
<rdfs:label xml:lang="en">reference list</rdfs:label>
<rdfs:seeAlso xml:lang="en">list of works cited</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Repository -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Repository">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000030"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#RepositoryName"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">a central location in which experimental protocols are stored and managed.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">a central location in which experimental protocols are stored and managed.</rdfs:comment>
<rdfs:label xml:lang="en">repository</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#RepositoryName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#RepositoryName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<obo:IAO_0000115 xml:lang="en">Title of the repository in which a resource was/will be stored and managed.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Title of the repository in which a resource was/will be stored and managed.</rdfs:comment>
<rdfs:label xml:lang="en">repository name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ResultsSection -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ResultsSection">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000314"/>
<obo:IAO_0000115 xml:lang="en">Results section is part of a document. In the context of a protocol, this section should show the output of a protocol.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Results section is part of a document. In the context of a protocol, this section should show the output of a protocol.</rdfs:comment>
<rdfs:label xml:lang="en">results section</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ReverseTranscriptase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ReverseTranscriptase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#EnzymeReagent"/>
<obo:IAO_0000112 xml:lang="en">HIV-1 reverse transcriptase
M-MLV reverse transcriptase
AMV reverse transcriptase</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">RNA-directed DNA-polymerase
RNA-directed DNA polymerase
RNA-dependent DNA-polymerase
RNA-dependent DNA polymerase</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Reverse_transcriptase</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.
Definition source: https://en.wikipedia.org/wiki/Reverse_transcriptase
Example of usage:
HIV-1 reverse transcriptase
M-MLV reverse transcriptase
AMV reverse transcriptase</rdfs:comment>
<rdfs:label xml:lang="en">reverse transcriptase</rdfs:label>
<rdfs:seeAlso xml:lang="en">RNA-directed DNA-polymerase
RNA-directed DNA polymerase
RNA-dependent DNA-polymerase
RNA-dependent DNA polymerase</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ReverseTranscription -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ReverseTranscription">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolInstruction"/>
<obo:IAO_0000115 xml:lang="en">In this step, total RNA or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">In this step, total RNA or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase.</rdfs:comment>
<rdfs:label xml:lang="en">reverse transcription</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ReverseTranscriptionPCRProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ReverseTranscriptionPCRProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#EnzimaticAmplificationProtocol"/>
<obo:IAO_0000115 xml:lang="en">reverse transcription PCR protocol is a step by step description of the procedure to reverse-transcribe and amplify RNA to cDNA.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">reverse transcription PCR protocol is a step by step description of the procedure to reverse-transcribe and amplify RNA to cDNA.</rdfs:comment>
<rdfs:label xml:lang="en">reverse transcription PCR protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ReviewerRole -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ReviewerRole">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/BFO_0000023"/>
<obo:IAO_0000115 xml:lang="en">a person who formally assesses or examines a document, for example, a protocol.</obo:IAO_0000115>
<rdfs:label xml:lang="en">reviewer role</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SampleMill -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SampleMill">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PhysicalDisruptionEquipmentAndSupplies"/>
<obo:IAO_0000115 xml:lang="en">sample mills cover a broad range of sample preparation equipment and supplies. The range includes homogenizers, blenders and mills for the preparation of high-fat, high-moisture, dry, frozen and fibrous samples.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">sample mills cover a broad range of sample preparation equipment and supplies. The range includes homogenizers, blenders and mills for the preparation of high-fat, high-moisture, dry, frozen and fibrous samples.</rdfs:comment>
<rdfs:label xml:lang="en">sample mill</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SaponinReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SaponinReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000112 xml:lang="en">surfactant that enhances penetration of proteins and other macromolecules through cell membranes; it also has been used as an adjuvant for vaccines.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Saponin is a compound containing one or more hydrophilic glycoside moieties combined with a lipophilic triterpenoid or steroid derivative. Found in particular abundance in plant species.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sapogenin glycosides
sapogenin glycoside
saponins</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Example of usage:
surfactant that enhances penetration of proteins and other macromolecules through cell membranes; it also has been used as an adjuvant for vaccines.
example usage source: http://www.sigmaaldrich.com/catalog/product/sigma/s4521?lang=en&region=CO</rdfs:comment>
<rdfs:comment xml:lang="en">Saponin is a compound containing one or more hydrophilic glycoside moieties combined with a lipophilic triterpenoid or steroid derivative. Found in particular abundance in plant species.
Definition source: CHEBI</rdfs:comment>
<rdfs:label xml:lang="en">saponin reagent</rdfs:label>
<rdfs:seeAlso xml:lang="en">sapogenin glycosides
sapogenin glycoside
saponins</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SarcosineReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SarcosineReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">A N-alkylglycine that is the N-methyl derivative of glycine. It is an intermediate in the metabolic pathway of glycine.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">SARCOSINE
Sarcosine
MeGly
(methylamino)acetic acid
sarcosinic acid
Sar
N-Methylglycine
(methylamino)ethanoic acid
methylaminoacetic acid
N-methylaminoacetic acid
C3H7NO2</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A N-alkylglycine that is the N-methyl derivative of glycine. It is an intermediate in the metabolic pathway of glycine.
Definition source: CHEBI</rdfs:comment>
<rdfs:label xml:lang="en">sarcosine reagent</rdfs:label>
<rdfs:seeAlso xml:lang="en">SARCOSINE
Sarcosine
MeGly
(methylamino)acetic acid
sarcosinic acid
Sar
N-Methylglycine
(methylamino)ethanoic acid
methylaminoacetic acid
N-methylaminoacetic acid
C3H7NO2</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SeventyFivePercentEthanol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SeventyFivePercentEthanol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#EthanolSolution"/>
<obo:IAO_0000115 xml:lang="en">An aqueous dilution of ethanol containing 75% ethanol by volume.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">75% Ethyl Alcohol
Ethyl Alcohol, 75%
Alcohol, Ethyl 75%
75% (v/v) ethanol
75 per cent alcohol
75% RNase-free ethanol
75% RNase free ethanol
75% EtOH</obo:IAO_0000118>
<rdfs:comment xml:lang="en">An aqueous dilution of ethanol containing 75% ethanol by volume.</rdfs:comment>
<rdfs:label xml:lang="en">75% ethanol</rdfs:label>
<rdfs:seeAlso xml:lang="en">75% Ethyl Alcohol
Ethyl Alcohol, 75%
Alcohol, Ethyl 75%
75% (v/v) ethanol
75 per cent alcohol
75% RNase-free ethanol
75% RNase free ethanol
75% EtOH</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SodiumLauroylSarcosinate -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SodiumLauroylSarcosinate">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SarcosineReagent"/>
<obo:IAO_0000112 xml:lang="en">Sarkosyl is effective in solubilizing functionally active membrane proteins, the major use of Sarkosyl has been to isolate DNA and RNA. The principal reason for this is that Sarkosyl is highly efficient in dissociating nucleosomes and ribosomes.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Sodium lauroyl sarcosinate also known as sarkosyl, is an ionic surfactant derived from sarcosine. In molecular biology experiments, sarkosyl is used to inhibit the initiation of DNA transcription.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sarkosyl
Sarkosyl
sarcosyl
Sarcosyl
N-Lauroylsarcosine
N-lauryl-sarcosine
N-Dodecanoyl-N-methylglycine</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Sodium_lauroyl_sarcosinate</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Sodium lauroyl sarcosinate also known as sarkosyl, is an ionic surfactant derived from sarcosine. In molecular biology experiments, sarkosyl is used to inhibit the initiation of DNA transcription.
Definition source: https://en.wikipedia.org/wiki/Sodium_lauroyl_sarcosinate
Example of usage:
Sarkosyl is effective in solubilizing functionally active membrane proteins, the major use of Sarkosyl has been to isolate DNA and RNA. The principal reason for this is that Sarkosyl is highly efficient in dissociating nucleosomes and ribosomes.</rdfs:comment>
<rdfs:label xml:lang="en">Sodium lauroyl sarcosinate</rdfs:label>
<rdfs:seeAlso xml:lang="en">sarkosyl
Sarkosyl
sarcosyl
Sarcosyl
N-Lauroylsarcosine
N-lauryl-sarcosine
N-Dodecanoyl-N-methylglycine</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SodiumSaltReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SodiumSaltReagent">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#AlkaliMetalSaltReagent"/>
<obo:IAO_0000115 xml:lang="en">Any alkali metal salt having sodium(1+) as the cation.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sodium salts
Natriumsalze
Natriumsalz</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">sodium salt reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SoftwareList -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SoftwareList">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000100"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/ERO_0000071"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">Software list is a data set of the names of softwares that are part of materials section of a protocol.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Software list is a data set of the names of softwares that are part of materials section of a protocol.</rdfs:comment>
<rdfs:label xml:lang="en">software list</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SoftwareName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SoftwareName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<owl:disjointWith rdf:resource="http://purl.org/net/SMARTprotocol#SolutionName"/>
<obo:IAO_0000112 xml:lang="en">LightCycler 480 Software.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Software name is a textual entity. Software name is the name of a software.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Software name is a textual entity. Software name is the name of a software.
Example of usage: LightCycler 480 Software.</rdfs:comment>
<rdfs:label xml:lang="en">software name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SolutionName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SolutionName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<obo:IAO_0000112 xml:lang="en">3M NaOH (1L)</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Solution name is a textual entity. Solution name is the name of a liquid preparation that contains at least 2 chemical substances, one playing the role of solvent and the other one playing the role of solute. If is applicable, the name of the solution should including the follow information: solution concentration, and final volume.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Solution name is a textual entity. Solution name is the name of a liquid preparation that contains at least 2 chemical substances, one playing the role of solvent and the other one playing the role of solute. If is applicable, the name of the solution should including the follow information: solution concentration, and final volume.
Example of usage: 3M NaOH (1L)</rdfs:comment>
<rdfs:label xml:lang="en">solution name</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SpecimenList -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SpecimenList">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000100"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/OBI_0000671"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/OBI_0100026"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">specimen list is a data set of the names or identifiers of the specimens that are part of materials section of a protocol.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sample list</obo:IAO_0000118>
<rdfs:comment xml:lang="en">specimen list is a data set of the names or identifiers of the specimens that are part of materials section of a protocol.</rdfs:comment>
<rdfs:label xml:lang="en">specimen list</rdfs:label>
<rdfs:seeAlso xml:lang="en">sample list</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SpecimenName -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SpecimenName">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42614"/>
<obo:IAO_0000115 xml:lang="en">Specimen name is a textual entity. Specimen name is the name of the organism, strain, line, ecotype, cultivar, etc. used in a protocol.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sample name</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Specimen name is a textual entity. Specimen name is the name of the organism, strain, line, ecotype, cultivar, etc. used in a protocol.</rdfs:comment>
<rdfs:label xml:lang="en">specimen name</rdfs:label>
<rdfs:seeAlso xml:lang="en">sample name</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SpecimenPreparationProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SpecimenPreparationProtocol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ExperimentalProtocol"/>
<obo:IAO_0000115 xml:lang="en">A specimen preparation protocol is a protocol used in a biological experiment and defines instructions on how a specimen or sample should be treated in a given experimental scenario.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sample preparation protocol</obo:IAO_0000118>
<rdfs:comment xml:lang="en">A specimen preparation protocol is a protocol used in a biological experiment and defines instructions on how a specimen or sample should be treated in a given experimental scenario.</rdfs:comment>
<rdfs:label xml:lang="en">specimen preparation protocol</rdfs:label>
<rdfs:seeAlso xml:lang="en">sample preparation protocol</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#StainExtractionBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#StainExtractionBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#LysisBuffer"/>
<obo:IAO_0000112 xml:lang="en">DNA purification</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Stain extraction buffer is a buffer used to digest and extract DNA.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">SEB
SEB buffer
Stain Extraction Buffer
SEB extraction buffer</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Stain extraction buffer is a buffer used to digest and extract DNA.
Example of usage: DNA purification</rdfs:comment>
<rdfs:label xml:lang="en">stain extraction buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">SEB
SEB buffer
Stain Extraction Buffer
SEB extraction buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#StainedDNA -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#StainedDNA">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProcessedDNA"/>
<obo:IAO_0000115 xml:lang="en">Stained DNA is a material entity. After the electrophoresis is complete, the molecules in the gel can be stained to make them visible. DNA may be visualized using ethidium bromide which, when intercalated into DNA, fluoresce under ultraviolet light. EtBr is a known mutagen, and safer alternatives are available, such as GelRed, which binds to the minor groove. SYBER Green I is another dsDNA stain. It is 25 times more sensitive, and possibly safer than EtBr, though there is no data addressing its mutagenicity or toxicity in humans. SYBER Safe is a variant of SYBR Green that has been shown to have low enough levels of mutagenicity and toxicity to be deemed nonhazardous waste under U.S. Federal regulations. If the molecules to be separated contain radioactivity, for example in DNA sequencing gel, an autoradiogram can be recorded of the gel. If the molecules to be separated not contain radioactivity in a DNA sequencing gel, it may be visualized using silver nitrate.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://en.wikipedia.org/wiki/Gel_electrophoresis</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Stained DNA is a material entity. After the electrophoresis is complete, the molecules in the gel can be stained to make them visible. DNA may be visualized using ethidium bromide which, when intercalated into DNA, fluoresce under ultraviolet light. EtBr is a known mutagen, and safer alternatives are available, such as GelRed, which binds to the minor groove. SYBER Green I is another dsDNA stain. It is 25 times more sensitive, and possibly safer than EtBr, though there is no data addressing its mutagenicity or toxicity in humans. SYBER Safe is a variant of SYBR Green that has been shown to have low enough levels of mutagenicity and toxicity to be deemed nonhazardous waste under U.S. Federal regulations. If the molecules to be separated contain radioactivity, for example in DNA sequencing gel, an autoradiogram can be recorded of the gel. If the molecules to be separated not contain radioactivity in a DNA sequencing gel, it may be visualized using silver nitrate.
Definition source: http://en.wikipedia.org/wiki/Gel_electrophoresis</rdfs:comment>
<rdfs:label xml:lang="en">stained DNA</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SteroidAcidReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SteroidAcidReagent">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SteroidReagent"/>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Any steroid substituted by at least one carboxy group.Annotations</obo:IAO_0000115>
<obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">steroid acids</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CHEBI</obo:IAO_0000412>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">steroid acid reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SteroidReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SteroidReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">Any of naturally occurring compounds and synthetic analogues, based on the cyclopenta[a]phenanthrene carbon skeleton, partially or completely hydrogenated; there are usually methyl groups at C-10 and C-13, and often an alkyl group at C-17. By extension, one or more bond scissions, ring expansions and/or ring contractions of the skeleton may have occurred. Natural steroids are derived biogenetically from triterpenoids.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<rdfs:label xml:lang="en">steroid reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#StorageCondition -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#StorageCondition">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">Store the buffer in foil covered dark coloured bottle at room temperature. Light sensitive. Can be used up to 2 months.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Storage condition is a message given to a user of a protocol about the shelf life (maximum storage time) and temperature of storage of a buffer, reagent or solution.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Storage condition is a message given to a user of a protocol about the shelf life (maximum storage time) and temperature of storage of a buffer, reagent or solution.
Example of usage: Store the buffer in foil covered dark coloured bottle at room temperature. Light sensitive. Can be used up to 2 months.</rdfs:comment>
<rdfs:label xml:lang="en">storage condition</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SubcellularProteinFractionationKitforTissues -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SubcellularProteinFractionationKitforTissues">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Kit"/>
<obo:IAO_0000115 xml:lang="en">subcellular protein fractionation kit for tissues includes five cellular components to obtain proteins.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">https://www.thermofisher.com/es/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/subcellular-protein-fractionation.html</obo:IAO_0000119>
<rdfs:comment xml:lang="en">subcellular protein fractionation kit for tissues includes five cellular components to obtain proteins.
Definition source:
https://www.thermofisher.com/es/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/subcellular-protein-fractionation.html</rdfs:comment>
<rdfs:label xml:lang="en">subcellular protein fractionation kit for tissues</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SulfateSaltReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SulfateSaltReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000118 xml:lang="en">sulphate salts
sulfate salts
sulphates
sulfates</obo:IAO_0000118>
<rdfs:label xml:lang="en">sulfate salt reagent</rdfs:label>
<rdfs:seeAlso xml:lang="en">sulphate salts
sulfate salts
sulphates
sulfates</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#SuperScriptReverseTranscriptase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#SuperScriptReverseTranscriptase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ReverseTranscriptase"/>
<obo:IAO_0000115 xml:lang="en">SuperScript reverse transcriptase (RT) is a family of enzymes widely used for cDNA synthesis.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Superscript II
SuperScript II
SuperScript III
Superscript III
SuperScript IV
Superscript IV
SuperScript II Reverse Transcriptase
SuperScript III Reverse Transcriptase
SuperScript IV Reverse Transcriptase
Superscript II RT
SuperScript II RT
Superscript III RT
SuperScript III RT
SuperScript II Reverse Transcriptase (RT)
SuperScript III Reverse Transcriptase (RT)
SuperScript IV Reverse Transcriptase (RT)</obo:IAO_0000118>
<rdfs:comment xml:lang="en">SuperScript reverse transcriptase (RT) is a family of enzymes widely used for cDNA synthesis.</rdfs:comment>
<rdfs:label xml:lang="en">SuperScript reverse transcriptase</rdfs:label>
<rdfs:seeAlso xml:lang="en">Superscript II
SuperScript II
SuperScript III
Superscript III
SuperScript IV
Superscript IV
SuperScript II Reverse Transcriptase
SuperScript III Reverse Transcriptase
SuperScript IV Reverse Transcriptase
Superscript II RT
SuperScript II RT
Superscript III RT
SuperScript III RT
SuperScript II Reverse Transcriptase (RT)
SuperScript III Reverse Transcriptase (RT)
SuperScript IV Reverse Transcriptase (RT)</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#TaqManBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#TaqManBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PCRBuffer"/>
<obo:IAO_0000115 xml:lang="en">TaqMan buffer is PCR buffer with ROX added for florescence.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">TaqMan Buffer
TaqMan master mix
TaqMan Master Mix
TaqMan Universal PCR Master Mix
TaqMan universal PCR master mix</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://cancer.ucsf.edu/_docs/cores/genome/protocols/TaqManBuffer.pdf</obo:IAO_0000119>
<rdfs:comment xml:lang="en">TaqMan buffer is PCR buffer with ROX added for florescence.
Definition source: http://cancer.ucsf.edu/_docs/cores/genome/protocols/TaqManBuffer.pdf</rdfs:comment>
<rdfs:label xml:lang="en">TaqMan buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">TaqMan Buffer
TaqMan master mix
TaqMan Master Mix
TaqMan Universal PCR Master Mix
TaqMan universal PCR master mix</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#TaqPolymerase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#TaqPolymerase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#EnzymeReagent"/>
<obo:IAO_0000115 xml:lang="en">Taq polymerase is a heat stable DNA-direct DNA polymerase from the bacteria Thermus aquaticus. It is widely used for the amplification of genes through the process of Polymerase Chain Reaction.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Taq Polymerase
Thermus aquaticus Polymerase
Taq DNA Polymerase
Taq1 Polymerase
Taq pol
Taq Enzyme
Taq enzyme
Taq DNA polymerase
Thermostable DNA polymerase
thermostable DNA polymerase
thermophilic bacterium Thermus aquaticus</obo:IAO_0000118>
<rdfs:comment xml:lang="en">Taq polymerase is a heat stable DNA-direct DNA polymerase from the bacteria Thermus aquaticus. It is widely used for the amplification of genes through the process of Polymerase Chain Reaction.</rdfs:comment>
<rdfs:label xml:lang="en">taq polymerase</rdfs:label>
<rdfs:seeAlso xml:lang="en">Taq Polymerase
Thermus aquaticus Polymerase
Taq DNA Polymerase
Taq1 Polymerase
Taq pol
Taq Enzyme
Taq enzyme
Taq DNA polymerase
Thermostable DNA polymerase
thermostable DNA polymerase
thermophilic bacterium Thermus aquaticus</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#TaqPolymeraseBuffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#TaqPolymeraseBuffer">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#PCRBuffer"/>
<obo:IAO_0000115 xml:lang="en">Taq polymerase buffer is a solution formulated to provide efficient activation of the enzyme Taq DNA polymerase, resulting in PCR amplification.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Taq DNA polymerase buffer
Taq buffer
Taq Buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">PERSON:Olga Giraldo</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Taq polymerase buffer is a solution formulated to provide efficient activation of the enzyme Taq DNA polymerase, resulting in PCR amplification.</rdfs:comment>
<rdfs:label xml:lang="en">Taq polymerase buffer</rdfs:label>
<rdfs:seeAlso xml:lang="en">Taq DNA polymerase buffer
Taq buffer
Taq Buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#TenPercentEthanol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#TenPercentEthanol">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#EthanolSolution"/>
<obo:IAO_0000115 xml:lang="en">An aqueous dilution of ethanol containing 10% ethanol by volume.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">10% Ethyl Alcohol
Ethyl Alcohol, 10%
Alcohol, Ethyl 10%
10% (v/v) ethanol
10 per cent alcohol
10% RNase-free ethanol
10% RNase free ethanol
10% EtOH</obo:IAO_0000118>
<rdfs:comment xml:lang="en">An aqueous dilution of ethanol containing 10% ethanol by volume.</rdfs:comment>
<rdfs:label xml:lang="en">10% ethanol</rdfs:label>
<rdfs:seeAlso xml:lang="en">10% Ethyl Alcohol
Ethyl Alcohol, 10%
Alcohol, Ethyl 10%
10% (v/v) ethanol
10 per cent alcohol
10% RNase-free ethanol
10% RNase free ethanol
10% EtOH</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Timing -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Timing">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">Steps 1-3, 30 min.; Steps 6-7, 2 h</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Timing is a message used to indicate the approximate time that a step, or set of steps, could take.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Timing is a message used to indicate the approximate time that a step, or set of steps, could take.
Example of usage: Steps 1-3, 30 min.; Steps 6-7, 2 h</rdfs:comment>
<rdfs:label xml:lang="en">timing</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#TitaniumTaqDNAPolymerase -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#TitaniumTaqDNAPolymerase">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#TaqPolymerase"/>
<obo:IAO_0000115 xml:lang="en">Titanium Taq is a mixture consisting of a 5’ exonuclease-deficient Taq polymerase, and TaqStart Antibody, a monoclonal antibody which inhibits Taq at ambient temperatures. TaqStart Antibody provides automatic hot start PCR.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">TITANIUM Taq DNA Polymerase</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://www.clontech.com/RO/Products/cDNA_Synthesis_and_Library_Construction/cDNA_and_Genomic_DNA/RACE-Ready_cDNA/ibcGetAttachment.jsp?cItemId=31113&fileId=5994516&sitex=10020:22372:US</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Titanium Taq is a mixture consisting of a 5’ exonuclease-deficient Taq polymerase, and TaqStart Antibody, a monoclonal antibody which inhibits Taq at ambient temperatures. TaqStart Antibody provides automatic hot start PCR.
Definition source: http://www.clontech.com/RO/Products/cDNA_Synthesis_and_Library_Construction/cDNA_and_Genomic_DNA/RACE-Ready_cDNA/ibcGetAttachment.jsp?cItemId=31113&fileId=5994516&sitex=10020:22372:US</rdfs:comment>
<rdfs:label xml:lang="en">Titanium Taq DNA Polymerase</rdfs:label>
<rdfs:seeAlso xml:lang="en">TITANIUM Taq DNA Polymerase</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#TitleOfProtocol -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#TitleOfProtocol">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<owl:disjointWith rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000115 xml:lang="en">Title of protocol is a subclass of textual entity. Title of protocol is the name of a protocol.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Title of protocol is a subclass of textual entity. Title of protocol is the name of a protocol.</rdfs:comment>
<rdfs:label xml:lang="en">title of the protocol</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Toxic -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Toxic">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122"/>
<obo:IAO_0000112 xml:lang="en">Avoid inhalation, ingestion or contact with skin, eyes or mucous membranes. Reference: Nature Protocols</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Of or relating to or caused by a toxin or poison 'suffering from exposure to toxic substances'</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://wordnetweb.princeton.edu/perl/webwn?s=toxic</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Of or relating to or caused by a toxin or poison 'suffering from exposure to toxic substances'
Definition source: http://wordnetweb.princeton.edu/perl/webwn?s=toxic
Example of usage: Avoid inhalation, ingestion or contact with skin, eyes or mucous membranes.</rdfs:comment>
<rdfs:label xml:lang="en">toxic</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Troubleshooting -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Troubleshooting">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000112 xml:lang="en">Problem: Low detection level for tested a miRNA (CT value>35) (Step 9 - 10) Possible reason: low abundant amiRNA, or low efficiency of low of polyA tailing and reverse transcription, or amplification condition is too stringent, or amiRNA is not correctly expressed or processed. Possible solutions: increase amount of cDNA template in PCR; use new polyA tailing and reverse transcription reagent, (...) or using low stringent PCR program; check PCR product sequence.</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A troubleshooting is a message given to a user of a protocol where common problems, possible causes, and solutions/methods of correction should be listed.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">A troubleshooting is a message given to a user of a protocol where common problems, possible causes, and solutions/methods of correction should be listed.
Example of usage:
Problem: Low detection level for tested a miRNA (CT value>35) (Step 9 - 10) Possible reason: low abundant amiRNA, or low efficiency of low of polyA tailing and reverse transcription, or amplification condition is too stringent, or amiRNA is not correctly expressed or processed. Possible solutions: increase amount of cDNA template in PCR; use new polyA tailing and reverse transcription reagent, (...) or using low stringent PCR program; check PCR product sequence.</rdfs:comment>
<rdfs:label xml:lang="en">troubleshooting</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ValidatorRole -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#ValidatorRole">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/BFO_0000023"/>
<obo:IAO_0000115 xml:lang="en">validator is a person who formally assesses or examines that the protocol can be understood and carried out correctly by users.</obo:IAO_0000115>
<rdfs:label xml:lang="en">validator role</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#VinylPolymerMacromoleculeReagent -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#VinylPolymerMacromoleculeReagent">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">A macromolecule composed of repeating -CHR-CH2- units.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">vinyl polymer
vinyl polymers</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">CHEBI</obo:IAO_0000119>
<rdfs:label xml:lang="en">vinyl polymer macromolecule reagent</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Volatile -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#Volatile">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122"/>
<obo:IAO_0000112 xml:lang="en">handle in well-ventilated charcoal-filtered hood. Reference: Nature Protocols</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">The property of a substance of changing readily from a solid or liquid to a vapor.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">The property of a substance of changing readily from a solid or liquid to a vapor.
Example of usage: handle in well-ventilated charcoal-filtered hood.</rdfs:comment>
<rdfs:label xml:lang="en">volatile</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#WaringBlenderPolytron -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#WaringBlenderPolytron">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#SampleMill"/>
<obo:IAO_0000115 xml:lang="en">Instrument used in mechanical lysis. Rotating blades grind and disperse cells and tissues</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">https://www.thermofisher.com/es/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/traditional-methods-cell-lysis.html</obo:IAO_0000119>
<rdfs:comment xml:lang="en">Instrument used in mechanical lysis. Rotating blades grind and disperse cells and tissues.
Definition source:
https://www.thermofisher.com/es/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/traditional-methods-cell-lysis.html</rdfs:comment>
<rdfs:label xml:lang="en">waring blender polytron</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#WashSolution -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#WashSolution">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830"/>
<rdfs:label xml:lang="en">wash solution</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#WashingNucleicAcids -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#WashingNucleicAcids">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolInstruction"/>
<obo:IAO_0000115 xml:lang="en">This step is to wash any residual salt away from the pelleted DNA/RNA.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://bitesizebio.com/253/the-basics-how-ethanol-precipitation-of-dna-and-rna-works/</obo:IAO_0000119>
<rdfs:comment xml:lang="en">This step is to wash any residual salt away from the pelleted DNA/RNA.
Definition source: http://bitesizebio.com/253/the-basics-how-ethanol-precipitation-of-dna-and-rna-works/</rdfs:comment>
<rdfs:label xml:lang="en">washing nucleic acids</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#YeastCell -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#YeastCell">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CL_0000521"/>
<rdfs:comment xml:lang="en">A general term denoting true fungi of the family Saccharomycetaceae that are widely distributed in substrates that contain sugars (such as fruits), and in soil, animal excreta, the vegetative parts of plants, etc.</rdfs:comment>
<rdfs:label xml:lang="en">yeast cell</rdfs:label>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#buffer -->
<owl:Class rdf:about="http://purl.org/net/SMARTprotocol#buffer">
<rdfs:subClassOf rdf:resource="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830"/>
<obo:IAO_0000112 xml:lang="en">The following could function as buffers when together in solution:
i) Acetic acid (weak organic acid w/ formula CH3COOH) and a salt containing its conjugate base, the acetate anion (CH3COO-), such as sodium acetate (CH3COONa).
ii) Pyridine (weak base w/ formula C5H5N) and a salt containing its conjugate acid, the pyridinium cation (C5H5NH+), such as Pyridinium Chloride.
iii) Ammonia (weak base w/ formula NH3) and a salt containing its conjugate acid, the ammonium cation, such as Ammonium Hydroxide (NH4OH).</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A buffer is a solution that can resist pH change upon the addition of an acidic or basic components. It is able to neutralize small amounts of added acid or base, thus maintaining the pH of the solution relatively stable. This is important for processes and/or reactions which require specific and stable pH ranges.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Buffer</obo:IAO_0000118>
<obo:IAO_0000119 xml:lang="en">http://chemwiki.ucdavis.edu/Physical_Chemistry/Acids_and_Bases/Buffers</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A buffer is a solution that can resist pH change upon the addition of an acidic or basic components. It is able to neutralize small amounts of added acid or base, thus maintaining the pH of the solution relatively stable. This is important for processes and/or reactions which require specific and stable pH ranges.
Definition source: http://chemwiki.ucdavis.edu/Physical_Chemistry/Acids_and_Bases/Buffers</rdfs:comment>
<rdfs:comment xml:lang="en">Example of usage:
The following could function as buffers when together in solution:
i) Acetic acid (weak organic acid w/ formula CH3COOH) and a salt containing its conjugate base, the acetate anion (CH3COO-), such as sodium acetate (CH3COONa).
ii) Pyridine (weak base w/ formula C5H5N) and a salt containing its conjugate acid, the pyridinium cation (C5H5NH+), such as Pyridinium Chloride.
iii) Ammonia (weak base w/ formula NH3) and a salt containing its conjugate acid, the ammonium cation, such as Ammonium Hydroxide (NH4OH).</rdfs:comment>
<rdfs:seeAlso xml:lang="en">Buffer</rdfs:seeAlso>
</owl:Class>
<!-- http://purl.org/obo/owl/SO#SO_0000121 -->
<owl:Class rdf:about="http://purl.org/obo/owl/SO#SO_0000121">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">A single stranded oligo used for polymerase chain reaction.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">forward DNA primer
DNA forward primer
forward primer oligonucleotide
forward primer oligo
forward primer polynucleotide
forward primer
Forward primer
forward primer sequence
Upper primer
upper primer
sense primer</obo:IAO_0000118>
<obo:IAO_0000118 xml:lang="en">forward DNA primer
DNA forward primer
forward primer oligonucleotide
forward primer oligo
forward primer polynucleotide
forward primer
forward primer sequence</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SNP Ontology</obo:IAO_0000412>
<rdfs:comment xml:lang="en">This term is mapped to MGED. Do not obsolete without consulting MGED ontology.</rdfs:comment>
<rdfs:label xml:lang="en">forward primer</rdfs:label>
</owl:Class>
<!-- http://purl.org/obo/owl/SO#SO_0000132 -->
<owl:Class rdf:about="http://purl.org/obo/owl/SO#SO_0000132">
<rdfs:subClassOf rdf:resource="http://purl.org/net/SMARTprotocol#Primer"/>
<obo:IAO_0000115 xml:lang="en">A single stranded oligo used for polymerase chain reaction.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">reverse primer oligonucleotide
reverse primer sequence
reverse primer
oligo DNA reverse primer
Reverse primer
reverse DNA primer
lower primer
Lower primer
antisense primer</obo:IAO_0000118>
<obo:IAO_0000118 xml:lang="en">reverse primer oligonucleotide
reverse primer sequence
reverse primer
oligo DNA reverse primer
reverse primer
reverse DNA primer</obo:IAO_0000118>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SNP Ontology</obo:IAO_0000412>
<rdfs:comment xml:lang="en">This term is mapped to MGED. Do not obsolete without consulting MGED ontology.</rdfs:comment>
<rdfs:label xml:lang="en">reverse primer</rdfs:label>
</owl:Class>
<!-- http://purl.org/spar/biro/BibliographicReference -->
<owl:Class rdf:about="http://purl.org/spar/biro/BibliographicReference">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000030"/>
<obo:IAO_0000115 xml:lang="en">An expression of a bibliographic record in a specific format, that references a particular textual or data publication (or a work in preparation for publication). Each bibliographic reference is a list of items containing entities that describe aspects of the referenced work - for example authors, title, journal, publication year - selected from the bibliographic record. Note that because different journal styles may require omission of elements of the bibliographic record in references included in a paper's reference list (e.g. the title, the identifier, the names of the eighth and subsequent authors), and because errors can be introduced when an author creates a bibliographic reference, the bibliographic reference to a published work may not be a complete or fully accurate expression of the information contained within the authoritative bibliographic record for that work.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">BiRO, the Bibliographic Reference Ontology</obo:IAO_0000412>
<terms:bibliographicCitation xml:lang="en"></terms:bibliographicCitation>
<rdfs:label xml:lang="en">bibliographic reference</rdfs:label>
</owl:Class>
<!-- http://semanticscience.org/resource/SIO_000052 -->
<owl:Class rdf:about="http://semanticscience.org/resource/SIO_000052">
<rdfs:subClassOf rdf:resource="http://semanticscience.org/resource/SIO_000070"/>
<obo:IAO_0000115 xml:lang="en">A quantity is an informational entity that gives the magnitude of a property.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">SIO</obo:IAO_0000412>
<rdfs:label xml:lang="en">quantity</rdfs:label>
</owl:Class>
<!-- http://semanticscience.org/resource/SIO_000070 -->
<owl:Class rdf:about="http://semanticscience.org/resource/SIO_000070">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000030"/>
<obo:IAO_0000115 xml:lang="en">A measurement value is a quantitative description that reflects the magnitude of some attribute.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">SIO</obo:IAO_0000412>
<rdfs:label xml:lang="en">measurement value</rdfs:label>
</owl:Class>
<!-- http://semanticscience.org/resource/SIO_001042 -->
<owl:Class rdf:about="http://semanticscience.org/resource/SIO_001042">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.obolibrary.org/obo/BCGO_0000183"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#FinalConcentration"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAlertMessage"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#Hint"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAlertMessage"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#StorageCondition"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasAlertMessage"/>
<owl:someValuesFrom rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://purl.org/net/SMARTprotocol#hasName"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#RecipeName"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#has_part"/>
<owl:someValuesFrom rdf:resource="http://semanticscience.org/resource/SIO_000070"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#RecipeList"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">A recipe is a set of instructions that describe how to prepare or make something.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">SIO</obo:IAO_0000412>
<rdfs:label xml:lang="en">recipe</rdfs:label>
</owl:Class>
<!-- http://www.ebi.ac.uk/efo/EFO_0000602 -->
<owl:Class rdf:about="http://www.ebi.ac.uk/efo/EFO_0000602">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/NCBITaxon_10090"/>
<obo:IAO_0000412 xml:lang="en">EFO</obo:IAO_0000412>
<rdfs:comment xml:lang="en">BALB/c is an albino, laboratory-bred strain of the house mouse from which a number of common substrains are derived. BALB/c mice are distributed globally, and are among the most widely used inbred strains used in animal experimentation.
Definition source: https://en.wikipedia.org/wiki/BALB/c</rdfs:comment>
<rdfs:label xml:lang="en">BALB/c</rdfs:label>
</owl:Class>
<!-- http://www.ebi.ac.uk/efo/EFO_0001327 -->
<owl:Class rdf:about="http://www.ebi.ac.uk/efo/EFO_0001327">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/NCBITaxon_10090"/>
<obo:IAO_0000115 xml:lang="en">A/J is a mouse strain as described in Jackson Laboratory</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://jaxmice.jax.org/strain/000646.html</obo:IAO_0000119>
<rdfs:comment xml:lang="en">A/J is a mouse strain as described in Jackson Laboratory.
Definition source: http://jaxmice.jax.org/strain/000646.html</rdfs:comment>
<rdfs:label xml:lang="en">A/J</rdfs:label>
</owl:Class>
<!-- http://www.ebi.ac.uk/efo/EFO_0004472 -->
<owl:Class rdf:about="http://www.ebi.ac.uk/efo/EFO_0004472">
<rdfs:subClassOf rdf:resource="http://www.ebi.ac.uk/efo/EFO_0005181"/>
<obo:IAO_0000115 xml:lang="en">C57BL/6 is a mouse strain as described in Jackson Laboratory strain index</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">C57/B6
C57BL6
B6
C57Black6
CB57
C57Black/6
C57Bl
C57Black
C57/BL6
C57Bl\6
C57
C57B/6
C57 Black/6</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">EFO</obo:IAO_0000412>
<rdfs:label xml:lang="en">C57BL/6</rdfs:label>
</owl:Class>
<!-- http://www.ebi.ac.uk/efo/EFO_0004964 -->
<owl:Class rdf:about="http://www.ebi.ac.uk/efo/EFO_0004964">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33697"/>
<obo:IAO_0000115 xml:lang="en">A RNA extract that is the output of an extraction process in which total RNA from either whole cells or from specific cellular compartment(s)/organelle(s) are isolated from a specimenA RNA extract that is the output of an extraction process in which total celluar and organelle RNA molecules are isolated from a specimen.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">total RNA extract
RNA extract</obo:IAO_0000118>
<rdfs:comment xml:lang="en">A RNA extract that is the output of an extraction process in which total RNA from either whole cells or from specific cellular compartment(s)/organelle(s) are isolated from a specimenA RNA extract that is the output of an extraction process in which total celluar and organelle RNA molecules are isolated from a specimen.</rdfs:comment>
<rdfs:label xml:lang="en">total RNA</rdfs:label>
<rdfs:seeAlso xml:lang="en">total RNA extract
RNA extract</rdfs:seeAlso>
</owl:Class>
<!-- http://www.ebi.ac.uk/efo/EFO_0005181 -->
<owl:Class rdf:about="http://www.ebi.ac.uk/efo/EFO_0005181">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/NCBITaxon_10090"/>
<obo:IAO_0000115 xml:lang="en">An inbred strain of mouse created in 1921 by C. C. Little at the Bussey Institute for Research in Applied Biology.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">EFO</obo:IAO_0000412>
<rdfs:label xml:lang="en">C57BL</rdfs:label>
</owl:Class>
<!-- http://www.ifomis.org/bfo/1.1#Entity -->
<owl:Class rdf:about="http://www.ifomis.org/bfo/1.1#Entity">
<rdfs:label xml:lang="en">entity</rdfs:label>
<rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">entity</rdfs:label>
</owl:Class>
<!-- http://www.ifomis.org/bfo/1.1/snap#Continuant -->
<owl:Class rdf:about="http://www.ifomis.org/bfo/1.1/snap#Continuant">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1#Entity"/>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Definition: An entity [bfo:Entity] that exists in full at any time in which it exists at all, persists through time while maintaining its identity and has no temporal parts.</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Examples: a heart, a person, the color of a tomato, the mass of a cloud, a symphony orchestra, the disposition of blood to coagulate, the lawn and atmosphere in front of our building</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Synonyms: endurant</rdfs:comment>
<rdfs:label xml:lang="en">continuant</rdfs:label>
</owl:Class>
<!-- http://www.ifomis.org/bfo/1.1/snap#DependentContinuant -->
<owl:Class rdf:about="http://www.ifomis.org/bfo/1.1/snap#DependentContinuant">
<owl:equivalentClass>
<owl:Class>
<owl:unionOf rdf:parseType="Collection">
<rdf:Description rdf:about="http://www.ifomis.org/bfo/1.1/snap#GenericallyDependentContinuant"/>
<rdf:Description rdf:about="http://www.ifomis.org/bfo/1.1/snap#SpecificallyDependentContinuant"/>
</owl:unionOf>
</owl:Class>
</owl:equivalentClass>
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#Continuant"/>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Definition: A continuant [snap:Continuant] that is either dependent on one or other independent continuant [snap:IndependentContinuant] bearers or inheres in or is borne by other entities.</rdfs:comment>
<rdfs:label xml:lang="en">dependent continuant</rdfs:label>
</owl:Class>
<!-- http://www.ifomis.org/bfo/1.1/snap#GenericallyDependentContinuant -->
<owl:Class rdf:about="http://www.ifomis.org/bfo/1.1/snap#GenericallyDependentContinuant">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#DependentContinuant"/>
<owl:disjointWith rdf:resource="http://www.ifomis.org/bfo/1.1/snap#SpecificallyDependentContinuant"/>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Definition: A continuant [snap:Continuant] that is dependent on one or other independent continuant [snap:IndependentContinuant] bearers. For every instance of A requires some instance of (an independent continuant [snap:IndependentContinuant] type) B but which instance of B serves can change from time to time.</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Examples: a certain PDF file that exists in different and in several hard drives</rdfs:comment>
<rdfs:label xml:lang="en">generically dependent continuant</rdfs:label>
</owl:Class>
<!-- http://www.ifomis.org/bfo/1.1/snap#IndependentContinuant -->
<owl:Class rdf:about="http://www.ifomis.org/bfo/1.1/snap#IndependentContinuant">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#Continuant"/>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Definition: A continuant [snap:Continuant] that is a bearer of quality [snap:Quality] and realizable entity [snap:RealizableEntity] entities, in which other entities inhere and which itself cannot inhere in anything.</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Examples: an organism, a heart, a leg, a person, a symphony orchestra, a chair, the bottom right portion of a human torso, the lawn and atmosphere in front of our building</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Synonyms: substantial entity</rdfs:comment>
<rdfs:label xml:lang="en">independent continuant</rdfs:label>
</owl:Class>
<!-- http://www.ifomis.org/bfo/1.1/snap#MaterialEntity -->
<owl:Class rdf:about="http://www.ifomis.org/bfo/1.1/snap#MaterialEntity">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#IndependentContinuant"/>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Definition: An independent continuant [snap:IndependentContinuant] that is spatially extended whose identity is independent of that of other entities and can be maintained through time. Note: Material entity [snap:MaterialEntity] subsumes object [snap:Object], fiat object part [snap:FiatObjectPart], and object aggregate [snap:ObjectAggregate], which assume a three level theory of granularity, which is inadequate for some domains, such as biology.</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Examples: collection of random bacteria, a chair, dorsal surface of the body</rdfs:comment>
<rdfs:label xml:lang="en">material entity</rdfs:label>
</owl:Class>
<!-- http://www.ifomis.org/bfo/1.1/snap#Quality -->
<owl:Class rdf:about="http://www.ifomis.org/bfo/1.1/snap#Quality">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#SpecificallyDependentContinuant"/>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Definition: A specifically dependent continuant [snap:SpecificallyDependentContinuant] that is exhibited if it inheres in an entity or entities at all (a categorical property).</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Examples: the color of a tomato, the ambient temperature of air, the circumference of a waist, the shape of a nose, the mass of a piece of gold, the weight of a chimpanzee</rdfs:comment>
<rdfs:label xml:lang="en">quality</rdfs:label>
</owl:Class>
<!-- http://www.ifomis.org/bfo/1.1/snap#SpecificallyDependentContinuant -->
<owl:Class rdf:about="http://www.ifomis.org/bfo/1.1/snap#SpecificallyDependentContinuant">
<rdfs:subClassOf rdf:resource="http://www.ifomis.org/bfo/1.1/snap#DependentContinuant"/>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Definition: A continuant [snap:Continuant] that inheres in or is borne by other entities. Every instance of A requires some specific instance of B which must always be the same.</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Examples: the mass of a cloud, the smell of mozzarella, the liquidity of blood, the color of a tomato, the disposition of fish to decay, the role of being a doctor, the function of the heart in the body: to pump blood, to receive de-oxygenated and oxygenated blood, etc.</rdfs:comment>
<rdfs:comment rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Synonyms: property, trope, mode</rdfs:comment>
<rdfs:label xml:lang="en">specifically dependent continuant</rdfs:label>
</owl:Class>
<!-- http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121 -->
<owl:Class rdf:about="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121">
<rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/IAO_0000300"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="http://www.obofoundry.org/ro/ro.owl#part_of"/>
<owl:someValuesFrom rdf:resource="http://purl.org/net/SMARTprotocol#MethodsSection"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An alert message is information, such as a warning or a caution that can be ignored by an automatic agent. The goal is to remind or alert the user.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">EXACT</obo:IAO_0000412>
<rdfs:label xml:lang="en">alert message</rdfs:label>
</owl:Class>
<!-- http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122 -->
<owl:Class rdf:about="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_122">
<rdfs:subClassOf rdf:resource="http://www.owl-ontologies.com/Ontology1184060740.owl#Class_121"/>
<obo:IAO_0000115 xml:lang="en">A caution is a message given to a user of a protocol about potentially dangerous properties of materials used in the experiment.</obo:IAO_0000115>
<obo:IAO_0000412 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">EXACT</obo:IAO_0000412>
<rdfs:label xml:lang="en">caution</rdfs:label>
</owl:Class>
<!--
///////////////////////////////////////////////////////////////////////////////////////
//
// Individuals
//
///////////////////////////////////////////////////////////////////////////////////////
-->
<!-- http://purl.org/net/SMARTprotocol#AnthonyJFreemont -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#AnthonyJFreemont">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#authorList1"/>
<rdfs:label xml:lang="en">Anthony J. Freemont</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#CrispinJMiller -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#CrispinJMiller">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#authorList1"/>
<rdfs:label xml:lang="en">Crispin J. Miller</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#EthylAlcohol -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#EthylAlcohol">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ReagentName"/>
<obo:OBI_0000304 rdf:resource="http://purl.org/net/SMARTprotocol#SigmaAldrich"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#reagentList1"/>
<rdfs:label xml:lang="en">Ethyl alcohol</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#Forceps -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#Forceps">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#equipmentList1"/>
<rdfs:label xml:lang="en">Forceps</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#HomogenizerBlades -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#HomogenizerBlades">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#equipmentList1"/>
<rdfs:label xml:lang="en">Homogenizer blades</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#Id1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#Id1">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ProtocolIdentifier"/>
<terms:description xml:lang="en">DOI: 10.2144/000113260</terms:description>
<rdfs:label xml:lang="en">protocol Id1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#Invitrogen -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#Invitrogen">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ManufacturerName"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#IsopropylAlcohol -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#IsopropylAlcohol">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ReagentName"/>
<obo:OBI_0000304 rdf:resource="http://purl.org/net/SMARTprotocol#SigmaAldrich"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#reagentList1"/>
<rdfs:label xml:lang="en">Isopropyl alcohol</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#JohnARadford -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#JohnARadford">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#authorList1"/>
<rdfs:label xml:lang="en">John A. Radford</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#KimMLinton -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#KimMLinton">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#authorList1"/>
<rdfs:label xml:lang="en">Kim M. Linton</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#RNAextraction01 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#RNAextraction01">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#RNAExtractionProtocol"/>
<adaptedFrom rdf:resource="http://purl.org/net/SMARTprotocol#provenance1"/>
<hasApplication rdf:resource="http://purl.org/net/SMARTprotocol#application1"/>
<hasAuthor rdf:resource="http://purl.org/net/SMARTprotocol#authorList1"/>
<hasExperimentalInputs rdf:resource="http://purl.org/net/SMARTprotocol#equipmentList1"/>
<hasExperimentalInputs rdf:resource="http://purl.org/net/SMARTprotocol#reagentList1"/>
<hasExperimentalInputs rdf:resource="http://purl.org/net/SMARTprotocol#tumorTissue"/>
<hasIdentifier rdf:resource="http://purl.org/net/SMARTprotocol#Id1"/>
<hasTitle rdf:resource="http://purl.org/net/SMARTprotocol#title1"/>
<terms:description xml:lang="en">protocol available here: http://www.biotechniques.com/protocols/Peer-Reviewed_Protocols/Extraction-of-total-RNA-from-freshfrozen-tissue-FT/biotechniques-181792.html</terms:description>
<rdfs:label xml:lang="en">RNA extraction 1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#Scalpel -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#Scalpel">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#equipmentList1"/>
<rdfs:label xml:lang="en">Scalpel</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#ScalpelHolder -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#ScalpelHolder">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#equipmentList1"/>
<rdfs:label xml:lang="en">Scalpel holder</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#SianDibben -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#SianDibben">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#authorList1"/>
<rdfs:label xml:lang="en">Sian Dibben</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#SigmaAldrich -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#SigmaAldrich">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ManufacturerName"/>
<rdfs:label xml:lang="en">Sigma-Aldrich</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#StuartDPepper -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#StuartDPepper">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#authorList1"/>
<rdfs:label xml:lang="en">Stuart D. Pepper</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#TRIZol -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#TRIZol">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ReagentName"/>
<obo:OBI_0000304 rdf:resource="http://purl.org/net/SMARTprotocol#Invitrogen"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#reagentList1"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#TissueStorageContainer -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#TissueStorageContainer">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentOrSuppliesName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#equipmentList1"/>
<rdfs:label xml:lang="en">Tissue storage container</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#YvonneHey -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#YvonneHey">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#AuthorName"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#authorList1"/>
<rdfs:label xml:lang="en">Yvonne Hey</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#application1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#application1">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ApplicationOfTheProtocol"/>
<terms:description rdf:datatype="http://www.w3.org/2000/01/rdf-schema#Literal">Methods comparison for high-resolution transcriptional analysis of archival material on Affymetrix Plus 2.0 and Exon 1.0 microarrays</terms:description>
<rdfs:label xml:lang="en">application 1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#author1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#author1">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#AnthonyJFreemont"/>
<rdfs:label>author1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#author2 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#author2">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#CrispinJMiller"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#author3 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#author3">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#JohnARadford"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#author4 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#author4">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#KimMLinton"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#author5 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#author5">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#SianDibben"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#author6 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#author6">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#StuartDPepper"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#author7 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#author7">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/OGI.owl#Author"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#YvonneHey"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#authorList1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#authorList1">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/IAO_0000321"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#AnthonyJFreemont"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#CrispinJMiller"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#JohnARadford"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#KimMLinton"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#SianDibben"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#StuartDPepper"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#YvonneHey"/>
<rdfs:label xml:lang="en">author list 1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#chloroform -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#chloroform">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ReagentName"/>
<obo:OBI_0000304 rdf:resource="http://purl.org/net/SMARTprotocol#SigmaAldrich"/>
<ro:part_of rdf:resource="http://purl.org/net/SMARTprotocol#reagentList1"/>
<rdfs:label xml:lang="en">Chloroform</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#equipmentList1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#equipmentList1">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#EquipmentAndSuppliesList"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#Forceps"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#HomogenizerBlades"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#Scalpel"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#ScalpelHolder"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#TissueStorageContainer"/>
<rdfs:label xml:lang="en">equipment list 1</rdfs:label>
<rdfs:label xml:lang="en">equipment-list1</rdfs:label>
<rdfs:label xml:lang="en">equipmentList1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#equipmentorsupplies1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#equipmentorsupplies1">
<rdf:type rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#HomogenizerBlades"/>
<rdfs:label xml:lang="en">equipment or supplies 1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#equipmentorsupplies2 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#equipmentorsupplies2">
<rdf:type rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#ScalpelHolder"/>
<rdfs:label xml:lang="en">equipment or supplies 2</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#equipmentorsupplies3 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#equipmentorsupplies3">
<rdf:type rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#TissueStorageContainer"/>
<rdfs:label xml:lang="en">equipment or supplies 3</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#equipmentorsupplies4 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#equipmentorsupplies4">
<rdf:type rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#Forceps"/>
<rdfs:label xml:lang="en">equipment or supplies 4</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#equipmentorsupplies5 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#equipmentorsupplies5">
<rdf:type rdf:resource="http://purl.bioontology.org/ontology/MSH/D004864"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#Scalpel"/>
<rdfs:label xml:lang="en">equipment or supplies 5</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#manufacturer1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#manufacturer1">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#Manufacturer"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#SigmaAldrich"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#manufacturer2 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#manufacturer2">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#Manufacturer"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#Invitrogen"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#provenance1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#provenance1">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ProvenanceOfTheProtocol"/>
<terms:description rdf:datatype="http://www.w3.org/2000/01/rdf-schema#Literal">The extraction method (steps 2–21) is taken from the method supplied with TRIzol reagent (Invitrogen, Paisley, UK).</terms:description>
<rdfs:label xml:lang="en">provenance 1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#reagent1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#reagent1">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#EthylAlcohol"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#reagent2 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#reagent2">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#IsopropylAlcohol"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#reagent3 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#reagent3">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#chloroform"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#reagent4 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#reagent4">
<rdf:type rdf:resource="http://purl.obolibrary.org/obo/CHEBI_33893"/>
<hasName rdf:resource="http://purl.org/net/SMARTprotocol#TRIZol"/>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#reagentList1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#reagentList1">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#ReagentList"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#EthylAlcohol"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#IsopropylAlcohol"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#TRIZol"/>
<ro:has_part rdf:resource="http://purl.org/net/SMARTprotocol#chloroform"/>
<rdfs:label xml:lang="en">reagent list 1</rdfs:label>
<rdfs:label xml:lang="en">reagentList1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#title1 -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#title1">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#TitleOfProtocol"/>
<terms:description xml:lang="en">Extraction of total RNA from fresh/frozen tissue (FT)</terms:description>
<rdfs:label xml:lang="en">title1</rdfs:label>
</owl:NamedIndividual>
<!-- http://purl.org/net/SMARTprotocol#tumorTissue -->
<owl:NamedIndividual rdf:about="http://purl.org/net/SMARTprotocol#tumorTissue">
<rdf:type rdf:resource="http://purl.org/net/SMARTprotocol#SpecimenName"/>
<rdfs:label xml:lang="en">tumor tissue</rdfs:label>
<rdfs:label xml:lang="en">tumor-tissue</rdfs:label>
</owl:NamedIndividual>
<!--
///////////////////////////////////////////////////////////////////////////////////////
//
// General axioms
//
///////////////////////////////////////////////////////////////////////////////////////
-->
<rdf:Description>
<rdf:type rdf:resource="http://www.w3.org/2002/07/owl#AllDisjointClasses"/>
<owl:members rdf:parseType="Collection">
<rdf:Description rdf:about="http://purl.obolibrary.org/obo/IAO_0000321"/>
<rdf:Description rdf:about="http://purl.org/net/SMARTprotocol#EquipmentAndSuppliesList"/>
<rdf:Description rdf:about="http://purl.org/net/SMARTprotocol#ReagentList"/>
<rdf:Description rdf:about="http://purl.org/net/SMARTprotocol#RecipeList"/>
<rdf:Description rdf:about="http://purl.org/net/SMARTprotocol#SoftwareList"/>
<rdf:Description rdf:about="http://purl.org/net/SMARTprotocol#SpecimenList"/>
</owl:members>
</rdf:Description>
</rdf:RDF>
<!-- Generated by the OWL API (version 4.2.6.20160910-2108) https://github.com/owlcs/owlapi -->