--- name: genomic-coordinates description: Convert genomic intervals between coordinate conventions, normalise and compare variant representations, and detect assembly or contig-naming mismatches before they corrupt an analysis. Use whenever coordinates cross a format, tool, or assembly boundary - converting between BED, GFF/GTF, VCF, SAM/BAM, WIG, PSL, genePred, Picard interval_list, or region strings; reconciling 0-based half-open with 1-based inclusive; left-aligning or trimming indels; checking whether two variant records describe the same change; mapping genomic to transcript, CDS, or protein positions; auditing a BED/GTF/VCF for convention violations; or diagnosing GRCh37 vs hg19 vs GRCh38 vs T2T, chr-prefix, and liftover problems. Triggers include "off by one", "0-based", "1-based", "half-open", "coordinate system", "left-align", "normalize variant", "bcftools norm", "chr prefix", "wrong genome build", "liftover", "REF mismatch", and "HGVS". license: MIT compatibility: Requires Python 3.11+. Scripts use only the standard library - no third-party packages and no network access. Variant normalisation needs a reference FASTA, and uses its .fai index when one is present. allowed-tools: Read Write Edit Bash metadata: version: "1.0" skill-author: K-Dense Inc. --- # Genomic Coordinates ## When to use Any time a coordinate crosses a boundary: between two file formats, between two tools, between two assemblies, or between the genome and a transcript. ## The rule **A coordinate is three facts, not one: the number, the convention it is written in, and the assembly it was measured against.** Carry all three or the number is not interpretable. Coordinate errors are the quietest class of bug in genomics. An off-by-one BED file parses, sorts, and intersects without complaint. A GRCh37 VCF joined against a GRCh38 annotation returns rows. A right-shifted indel simply fails to match its entry in ClinVar, and the result is a variant reported as novel. Nothing raises an error; the answer is just wrong, and it is wrong in a direction that looks plausible. So: convert with the table, not from memory, and verify against the reference whenever a reference is available. ## The two conversions ``` 1-based inclusive -> 0-based half-open : start - 1, end 0-based half-open -> 1-based inclusive : start + 1, end ``` The end coordinate never moves. If a conversion changed both numbers, it is wrong. ## Which format is which | 0-based, half-open | 1-based, inclusive | | --- | --- | | BED, bedGraph, bigWig, narrowPeak | GFF3, GTF, VCF | | BAM/CRAM (binary POS) | SAM (text POS) | | PSL, genePred, refFlat | WIG, Picard interval_list | | MAF (UCSC multiple alignment) | MAF (TCGA mutation annotation) | | PyRanges, pybedtools | GRanges/IRanges, samtools & UCSC & Ensembl region strings | Both "MAF" formats exist, they mean different things, and they disagree. UCSC serves 0-based files through a 1-based browser box. `references/format-conventions.md` has the full table with per-format detail. ```bash cd skills/genomic-coordinates/scripts python3 convert_coords.py --list # the table python3 convert_coords.py --from bed --to gff chr1 999 1000 python3 convert_coords.py --from ucsc --to bed "chr7:5,530,601-5,530,625" python3 convert_coords.py --from granges --to pyranges --input regions.tsv ``` ``` contig input output length status detail chr7 chr7:5530601-5530625 5530600-5530625 25 ok ``` Zero-length BED features (`chromStart == chromEnd`, a legal insertion point) are reported as `unrepresentable` rather than converted to `end = start - 1`. Exit code is 1 when any interval is degenerate or invalid. ## Variants are not intervals A VCF `POS` for an indel is the **anchor base** — the base *before* the event, itself unchanged. And the same change can be written many ways: `chr1:7:CAC:C`, `chr1:3:CAC:C` and `chr1:2:GCA:G` are one deletion. Joining, deduplicating, or looking up variants before normalising loses real matches silently, and it loses them preferentially in repeats, where indels concentrate. Normalise — trim to parsimony, then left-align against the reference — before any comparison: ```bash python3 normalize_variant.py --fasta ref.fa chr1 7 CAC C python3 normalize_variant.py --fasta ref.fa --split --input cohort.vcf python3 normalize_variant.py --fasta ref.fa --compare chr1:7:CAC:C chr1:2:GCA:G ``` ``` input normalized type pos_shift ref_check changed chr1:7:CAC:C chr1:2:GCA:G deletion 5 ok yes ``` Every record's `REF` is checked against the FASTA first. A `MISMATCH` means the variants and the reference are different assemblies — stop and run `check_contigs.py` rather than adjusting coordinates. Multi-allelic records must be split with `--split` **before** normalising, never after. HGVS shifts indels the opposite way, 3'-most along the transcript. For a minus-strand gene that is the opposite genomic direction from VCF's left-alignment. Details and the full procedure: `references/variant-representation.md`. ## Check the assembly before trusting a join ```bash python3 check_contigs.py --identify unknown.fa.fai python3 check_contigs.py variants.vcf annotation.gtf --genome GRCh38.fa.fai ``` ``` file kind contigs naming assembly detail ref.fa.fai sizes 25 plain GRCh37 24/24 primary chromosome lengths match; chrM is 16569 bp, i.e. GRCh37/38 (rCRS MT) ``` The script reads `.fai`, `.chrom.sizes`, VCF headers, SAM headers, FASTA, BED, and GTF/GFF, identifies the assembly from primary-chromosome lengths, and reports every reason a join between two files would go wrong: naming mismatch, length conflict, coordinates past a contig end, contigs present in one file only. Exit code 1 on any incompatibility. **GRCh37 and hg19 differ only in the mitochondrion** — 16,569 bp (rCRS) versus 16,571 bp. Nuclear coordinates are identical, so a mixed pipeline runs fine and only the mtDNA results are wrong. `check_contigs.py` reports which one it found. Builds, naming schemes, ALT contigs, and liftover pitfalls: `references/reference-builds.md`. ## Audit a file against its own format ```bash python3 audit_intervals.py peaks.bed python3 audit_intervals.py gencode.gtf --genome hg38.chrom.sizes python3 audit_intervals.py cohort.vcf --genome GRCh38.fa.fai ``` Looks for the evidence that a coordinate mistake leaves behind: | Finding | What it proves | | --- | --- | | `start_below_one` in GFF/GTF | 0-based data in a 1-based file; everything is one base left | | `many_zero_length` in BED | 1-based single-base features written into a 0-based file | | `past_contig_end` | wrong assembly, or an off-by-one at the contig edge | | `mixed_contig_naming` | any join will silently match one subset | | `first_block_offset` | BED12 `blockStarts` written as absolute coordinates | | `not_parsimonious` | untrimmed alleles; normalise before joining | | `bad_alt_allele` | Ensembl/VEP `-` notation in a VCF, which has no anchor base | Exit code 1 on any fatal finding, so it works as a CI gate on a data directory. ## Transcript, CDS, and protein positions `c.742` and `chr17:7,674,220` are both "position", and neither converts to the other by arithmetic. Transcript coordinates count spliced bases in transcription order — decreasing genomic coordinate on the minus strand — and `c.1` is the `A` of the initiator `ATG`, not the start of the transcript. The rules that get mis-remembered: there is no `c.0`; 5' UTR positions are negative and 3' UTR positions take a `*`; GFF phase is the bases to *remove* to reach the next codon, not `start % 3`; and a `c.` description is meaningless without a versioned transcript accession, because the same variant numbers differently in each transcript. `references/transcript-coordinates.md` has the conversion procedure and the boundary cases. Do the conversion with a tool that holds the transcript model — VEP, `bcftools csq`, Mutalyzer, the `hgvs` package — not by hand. ## Reporting results State the assembly next to the coordinates, every time. `chr7:5,530,601-5,530,625` is not a location; `chr7:5,530,601-5,530,625 (GRCh38)` is. Say which convention a coordinate column is in, in the column header or the file's documentation. When a conversion produced a result, say which direction it went. ## References - `references/format-conventions.md` — every format's convention, with per-format detail, BED12 block rules, region-string syntax, and tool behaviour. - `references/variant-representation.md` — VCF allele conventions, the normalisation algorithm, equivalence checking, multi-allelic splitting, and how HGVS disagrees with VCF. - `references/reference-builds.md` — build signatures, GRCh37 vs hg19, ALT contigs, naming schemes, and liftover failure modes. - `references/transcript-coordinates.md` — genomic ↔ transcript ↔ CDS ↔ protein, HGVS numbering, phase, and transcript choice.