TI Immobilization of Laccase by Alkali-Etched Bimetallic CoCu-MOF To Enhance Enzyme Loading and Congo Red Degradation EA JUN 2023
AB In this work, the strategy of immobilizing enzymes inbimetallic-organicframeworks was adopted to overcome the disadvantages of free laccases.The surface amino-silanizing of bimetallic CoCu-MOF-H hydrothermallysynthesized was performed by (3-Aminopropyl)triethoxysilane (APTES).Then, glutaraldehyde was used as the cross-linking agent, laccasewas covalently grafted onto CoCu-MOF-H-APTES to prepare Lac-CoCu-MOF-H-APTE.In addition, CoCu-MOF-OH also was synthesized by alkali etching ofCoCu-MOF-H, and Lac-CoCu-MOF-OH-APTES composites were obtained bya similar strategy. The result showed that the relative enzyme activityof Lac-CoCu-MOF-OH-APTES exhibited 264.02% (1.8 times than that ofLac-CoCu-MOF-H-APTES) after six cycles of stability tests, while thefree enzyme was almost inactivated. Moreover, the Congo red (CR) removalrate of Lac-CoCu-MOF-OH-APTES exceeded 95% within 1 h and exceeded89.18% after six cycles at pH 3.5 and 50 degrees C. This work has thepotential to provide a broader application prospect for CR degradationby laccase in the future.
