SERIES = 3; EXT = "czi"; folder = getDir("Select the input folder"); files = getFileList(folder); outFolder = folder + "channels" + File.separator; setBatchMode(true); if (!File.exists(outFolder)) File.makeDirectory(outFolder); for (i = 0; i < files.length; i++) { file = folder + files[i]; if (File.isDirectory(file)) continue; if (!endsWith(file, EXT)) continue; print(file); run("Bio-Formats", "open=["+file+"] color_mode=Default rois_import=[ROI manager] split_channels view=Hyperstack stack_order=XYCZT series_"+SERIES); imageTitles = getList("image.titles"); nrOfChannels = imageTitles.length; for (j = 0; j < nrOfChannels; j++) { name = getTitle(); save(outFolder + name + ".tif"); close(); } } setBatchMode("exit and display"); print("Conversion finished");